Search

Your search keyword '"Paviolo, Natalia Soledad"' showing total 10 results
Start Over Author "Paviolo, Natalia Soledad"
10 results on '"Paviolo, Natalia Soledad"'

4. Correction: Inhibitors of Rho kinases (ROCK) induce multiple mitotic defects and synthetic lethality in BRCA2-deficient cells

Author

Martino, Julieta, primary, Siri, Sebastián Omar, additional, Calzetta, Nicolás Luis, additional, Paviolo, Natalia Soledad, additional, Garro, Cintia, additional, Pansa, Maria F, additional, Carbajosa, Sofía, additional, Brown, Aaron C, additional, Bocco, José Luis, additional, Gloger, Israel, additional, Drewes, Gerard, additional, Madauss, Kevin P, additional, Soria, Gastón, additional, and Gottifredi, Vanesa, additional

Published
2023
Full Text
View/download PDF

5. Inhibitors of Rho kinases (ROCK) induce multiple mitotic defects and synthetic lethality in BRCA2-deficient cells

Author

Martino, Julieta, primary, Siri, Sebastián Omar, primary, Calzetta, Nicolás Luis, additional, Paviolo, Natalia Soledad, additional, Garro, Cintia, additional, Pansa, Maria F, additional, Carbajosa, Sofía, additional, Brown, Aaron C, additional, Bocco, José Luis, additional, Gloger, Israel, additional, Drewes, Gerard, additional, Madauss, Kevin P, additional, Soria, Gastón, additional, and Gottifredi, Vanesa, additional

Published
2023
Full Text
View/download PDF

6. Author response: Inhibitors of Rho kinases (ROCK) induce multiple mitotic defects and synthetic lethality in BRCA2-deficient cells

Author

Martino, Julieta, primary, Siri, Sebastián Omar, primary, Calzetta, Nicolás Luis, additional, Paviolo, Natalia Soledad, additional, Garro, Cintia, additional, Pansa, Maria F, additional, Carbajosa, Sofía, additional, Brown, Aaron C, additional, Bocco, José Luis, additional, Gloger, Israel, additional, Drewes, Gerard, additional, Madauss, Kevin P, additional, Soria, Gastón, additional, and Gottifredi, Vanesa, additional

Published
2022
Full Text
View/download PDF

8. Interstitial Telomeric Sequences Are Not Preferentially Involved in the Chromosome Damage Induced by the Methylating Compound Streptozotocin in Chinese Hamster Cells

Author

Quiroga, Ivana Yoseli, Paviolo, Natalia Soledad, and Bolzan, Alejandro Daniel

Subjects
Ciencias Biológicas, AlLKYLATING COMPOUNDS, TELOMERE, TELOMERE PNA-FISH, CHO CELLS, Bioquímica y Biología Molecular, CHROMOSOMAL ABERRATIONS, CIENCIAS NATURALES Y EXACTAS
Abstract

The effect of the methylating compound streptozotocin (STZ) on interstitial telomeric sequences (ITSs) was investigated in Chinese hamster ovary (CHO) cells by using peptide nucleic acid-fluorescence in situ hybridization with a pantelomeric probe. Cells were exposed to increasing concentrations of STZ, and chromosomal aberrations were analyzed at the first mitosis after treatment. The frequency of chromosomal aberrations directly involving ITSs increased in STZ-treated cells by a factor of 2.6 (2 mM) and 3.6 (4 mM) when compared with the frequency of these aberrations in control cells (P < 0.05). However, no significant differences were found between control and exposed cells in the percentage of aberrations directly involving ITSs, demonstrating that these repeat regions were not preferentially involved in the chromosome damage induced by STZ. In addition, STZ did not alter telomerase activity, suggesting that this enzyme may not be involved in the induction of chromosomal aberrations by this compound. Environ. Mol. Mutagen. 54:147–152, 2013. Fil: Quiroga, Ivana Yoseli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina Fil: Paviolo, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina Fil: Bolzan, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina

Published
2013
Full Text
View/download PDF

10. Persistent double strand break accumulation does not precede cell death in an Olaparib-sensitive BRCA-deficient colorectal cancer cell model.

Author

Paviolo NS, Vega MB, Pansa MF, García IA, Calzetta NL, Soria G, and Gottifredi V

Abstract

The poly (adenosine diphosphate (ADP)-ribosyl) polymerase inhibitors (PARPi) selectively kill cancer cells with BRCA1 or BRCA2 (BRCA)-mutations. It has been proposed that cell death induction after PARPi depends on unrepaired double strand breaks (DSBs) that accumulate due to the homologous recombination deficiency of BRCA-mutated cells. Such accumulation of DSBs is inferred mainly from the high levels of DNA damage markers like phosphorylated histone H2AX. Herein, we developed a model of isogenic cell lines to show that depletion of BRCA causes PARPi-triggered cell death, replication stress (phosphorylated-H2AX and 53BP1 foci), and genomic instability. However, persistent DSBs accumulation was not detected under the same experimental conditions. Hence, at least in this cellular model, the trigger for cell death in PARPi-treated BRCA-depleted samples is not the accumulation of unrepaired DSBs. Instead, cell death better correlates with a rapid and aberrant resolution of DSBs by error-prone pathways that leads to severe chromosomic aberrations. Therefore, our results suggest that in PARPi-treated BRCA-deficient cells, chromosome aberrations may dually trigger both genomic instability and cell death.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results