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20 results on '"Paviati, Elisa"'

2. Preanalytical Impact of Incomplete K 2 EDTA Blood Tube Filling in Molecular Biology Testing.

Author

Benati, Marco, Pighi, Laura, Paviati, Elisa, Visconti, Sara, Lippi, Giuseppe, and Salvagno, Gian Luca

Subjects
MOLECULAR biology, NUCLEIC acids, GENE expression, TUBES, RNA
Abstract

Background and aims: The aim of this study was to investigate the possible preanalytical effect of incomplete filling of blood tubes on molecular biology assays. Materials and methods: The study population consisted of 13 healthy volunteers from whom 11 mL of whole blood was collected and then distributed in different volumes (1.5, 3.0, and 6.0 mL, respectively) into three 6.0 mL spray-dried and evacuated K2EDTA blood tubes. Automated RNA extraction was performed using the Maxwell® CSC RNA Blood Kit. DNA was extracted with a MagCorePlusII, with concomitant measurement of glyceralde-hyde-3-phosphate dehydrogenase (GAPDH) gene expression. The nucleic acid concentration was calculated using the NanoDrop 1000 spectrophotometer, and purity was assessed using A260/280 and A260/230 absorbance ratios. Results: The RNA concentration was higher in the tubes filled with 1.5 and 3.0 mL of blood than in the reference 6 mL filled tube. The RNA 260/280 and RNA 260/230 ratios did not differ significantly between the differently filled blood tubes. The DNA concentration remained constant in the differently filled tubes. Compared to the 6.0 mL reference filled tube, the 1.5 mL and 3.0 mL filled blood tubes displayed a lower DNA 260/280 nm ratio. The DNA 260/230 ratio did not differ significantly in any of the variously filled tubes. Compared to the 6.0 mL reference filled blood tube, the 1.5 mL and 3.0 mL filled blood tubes showed a significant increase in the GAPDHcycle threshold. Conclusions: Our results suggest that underfilling of K2EDTA blood tubes may be a modest but analytically significant source of bias in molecular biology testing. [ABSTRACT FROM AUTHOR]

Published
2024
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7. The impact of sensitive KIT D816V detection on recognition of Indolent Systemic Mastocytosis

Author

De Matteis, Giovanna, Zanotti, Roberta, Colarossi, Sabrina, De Benedittis, Caterina, Garcia-Montero, Andrès, Bonifacio, Massimiliano, Sartori, Marta, Aprili, Fiorenza, Caruso, Beatrice, Paviati, Elisa, Carli, Giuseppe, Perbellini, Omar, Zamò, Alberto, Bonadonna, Patrizia, Pizzolo, Giovanni, Guidi, Giancesare, Martinelli, Giovanni, and Soverini, Simona

Published
2015
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10. Telomere shortening and PCDH10 promoter methylation in colorectal cancer mucosae

Author

Benati, Marco, Danese, Elisa, Montagnana, Martina, Paviati, Elisa, Anna Maria Minicozzi, Milena, Gusella, Pasini, Felice, and Lippi, Giuseppe

Subjects
Telomere shortening, PCDH10, promoter, methylation, colorectal cancer, promoter, PCDH10, colorectal cancer, methylation, Telomere shortening
Published
2019

11. Influence of middle-distance running on muscular micro RNAs

Author

Danese, Elisa, primary, Benati, Marco, additional, Sanchis-Gomar, Fabian, additional, Tarperi, Cantor, additional, Salvagno, Gian Luca, additional, Paviati, Elisa, additional, Montagnana, Martina, additional, Schena, Federico, additional, and Lippi, Giuseppe, additional

Published
2018
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16. Comparison of Genetic and Epigenetic Alterations of Primary Tumors and Matched Plasma Samples in Patients with Colorectal Cancer

Author

Danese, Elisa, primary, Minicozzi, Anna Maria, additional, Benati, Marco, additional, Montagnana, Martina, additional, Paviati, Elisa, additional, Salvagno, Gian Luca, additional, Lima-Oliveira, Gabriel, additional, Gusella, Milena, additional, Pasini, Felice, additional, Lippi, Giuseppe, additional, and Guidi, Gian Cesare, additional

Published
2015
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17. Improved Detection of the KIT D816V Mutation Using a Real-Time PCR Assay Allows a Finer Recognition of Patients with Indolent Systemic Mastocytosis

Author

Bonifacio, Massimiliano, primary, De Matteis, Giovanna, additional, De Benedittis, Caterina, additional, Perbellini, Omar, additional, Zamò, Alberto, additional, Bonadonna, Patrizia, additional, Paviati, Elisa, additional, Artuso, Anna, additional, Soverini, Simona, additional, Pizzolo, Giovanni, additional, Martinelli, Giovanni, additional, and Zanotti, Roberta, additional

Published
2011
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19. The impact of sensitive KIT D816V detection on recognition of Indolent Systemic Mastocytosis

Author

Fiorenza Aprili, Elisa Paviati, Alberto Zamò, Giovanni Pizzolo, Giovanna De Matteis, Massimiliano Bonifacio, Giuseppe Carli, Marta Sartori, Andrés C. García-Montero, Gian Cesare Guidi, Giovanni Martinelli, Simona Soverini, Roberta Zanotti, Omar Perbellini, Caterina De Benedittis, Patrizia Bonadonna, Beatrice Caruso, Sabrina Colarossi, De Matteis, Giovanna, Zanotti, Roberta, Colarossi, Sabrina, De Benedittis, Caterina, Garcia-Montero, Andrè, Bonifacio, Massimiliano, Sartori, Marta, Aprili, Fiorenza, Caruso, Beatrice, Paviati, Elisa, Carli, Giuseppe, Perbellini, Omar, Zamò, Alberto, Bonadonna, Patrizia, Pizzolo, Giovanni, Guidi, Giancesare, Martinelli, Giovanni, and Soverini, Simona

Subjects
Male, Cancer Research, Pathology, D-HPLC, Bone Marrow, Cytology, Outpatient clinic, Sequencing, Systemic mastocytosis, Chromatography, High Pressure Liquid, Aged, 80 and over, Sanger sequencing, Reverse Transcriptase Polymerase Chain Reaction, Medicine (all), KIT, Hematology, Middle Aged, Prognosis, Proto-Oncogene Proteins c-kit, Real-time polymerase chain reaction, D816V, Oncology, Monoclonal, symbols, Female, Real time PCR, Restriction fragment length polymorphism, Polymorphism, Restriction Fragment Length, Human, Adult, medicine.medical_specialty, Prognosi, Real-Time Polymerase Chain Reaction, Follow-Up Studie, Young Adult, symbols.namesake, Mastocytosis, Systemic, Biomarkers, Tumor, medicine, Humans, Point Mutation, RNA, Messenger, Aged, Cutaneous Mastocytosis, business.industry, medicine.disease, Systemic mastocytosi, business, Follow-Up Studies
Abstract

Patients with Systemic Mastocytosis (SM) need a highly sensitive diagnostic test for D816V detection of the KIT receptor gene.Along with histology/cytology and flow cytometry evaluation, bone marrow (BM) from 110 consecutive adult patients referred with a suspicion of SM to Multidisciplinary Outpatient Clinic for Mastocytosis in Verona were tested both by Amplification Refractory Mutation System Reverse Transcriptase quantitative real time Polymerase Chain Reaction (ARMS-RT-qPCR) and RT-PCR. +. Restriction Fragment Length Polymorphism (RFLP) followed by Denaturing-High Performance Liquid Chromatography (D-HPLC) and Sanger sequencing.ARMS-RT-qPCR identified D816V mutation in 77 patients, corresponding to 100% of cases showing CD25+ mast cells (MCs) whereas RT-PCR+RFLP/D-HPLC+sequencing revealed D816V mutations in 47 patients.According to the 2008 WHO criteria 75 SM, 1 Cutaneous Mastocytosis (CM), 1 monoclonal MC activation syndrome (MMAS), and 1 SM Associated with Haematologic Non-Mast Cell Disorder (SM-AHNMD) were diagnosed. Seventeen out 75 SM patients (23%) would have not satisfied sufficient WHO criteria on the basis of the sole RT-PCR+RFLP: these patients had significantly lower serum tryptase levels and amount of CD25+ MCs.Therefore, ARMS-RT-qPCR might result particularly useful, in patients that do not fulfil major BM histological criterion, for the recognition of indolent SM with a very low MC burden.

Published
2015

20. Long-term persistence of molecular response after discontinuation of interferon-alpha in two patients with chronic myeloid leukaemia.

Author

Veneri D, Tecchio C, De Matteis G, Paviati E, Benati M, Franchini M, and Pizzolo G

Subjects
Adult, Fusion Proteins, bcr-abl genetics, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics, Male, Middle Aged, RNA, Messenger genetics, Remission Induction, Immunologic Factors therapeutic use, Interferon-alpha therapeutic use, Leukemia, Myelogenous, Chronic, BCR-ABL Positive drug therapy
Published
2012
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