12 results on '"Paulson AL"'
Search Results
2. Machining Performance Optimization of FSW Using ANN-based PCA - A Hybrid approach for AA6061
- Author
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Reddy G. Sheelam Balavardhan, Kumaraswamy Singam, Paulson Aligi, Saipradeep P., Kumar Anshuman, and Subbiah Ram
- Subjects
fsw ,aa6061 ,uts ,pca ,ann ,Environmental sciences ,GE1-350 - Abstract
In this study, Friction stir welding (FSW) of AA6061 shows the importance of machining parameters such as tool rotational speed (TRS), feed rate (FR) and Tool Pressure angle (TPA). The machining performance has been measured through the ultimate tensile strength (UTS) and Vickers hardness (VH). The Taguchi’s philosophy has been considered in designing the experiment. The machining characteristics were analyzed using the main effect plot and analysis of variance (ANOVA). A principal component analysis (PCA) based composite principal component (CPC) has been used to optimise multi-response. The TRS has been found to be the most significant parameter for obtaining the optimum parameter setting. The performance has been enhanced using ANN technique of the process.
- Published
- 2024
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3. Body size interacts with the structure of the central nervous system: A multi-center in vivo neuroimaging study.
- Author
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Labounek R, Bondy MT, Paulson AL, Bédard S, Abramovic M, Alonso-Ortiz E, Atcheson NT, Barlow LR, Barry RL, Barth M, Battiston M, Büchel C, Budde MD, Callot V, Combes A, De Leener B, Descoteaux M, de Sousa PL, Dostál M, Doyon J, Dvorak AV, Eippert F, Epperson KR, Epperson KS, Freund P, Finsterbusch J, Foias A, Fratini M, Fukunaga I, Gandini Wheeler-Kingshott CAM, Germani G, Gilbert G, Giove F, Grussu F, Hagiwara A, Henry PG, Horák T, Hori M, Joers JM, Kamiya K, Karbasforoushan H, Keřkovský M, Khatibi A, Kim JW, Kinany N, Kitzler H, Kolind S, Kong Y, Kudlička P, Kuntke P, Kurniawan ND, Kusmia S, Laganà MM, Laule C, Law CSW, Leutritz T, Liu Y, Llufriu S, Mackey S, Martin AR, Martinez-Heras E, Mattera L, O'Grady KP, Papinutto N, Papp D, Pareto D, Parrish TB, Pichiecchio A, Prados F, Rovira À, Ruitenberg MJ, Samson RS, Savini G, Seif M, Seifert AC, Smith AK, Smith SA, Smith ZA, Solana E, Suzuki Y, Tackley GW, Tinnermann A, Valošek J, Van De Ville D, Yiannakas MC, Weber KA 2nd, Weiskopf N, Wise RG, Wyss PO, Xu J, Cohen-Adad J, Lenglet C, and Nestrašil I
- Abstract
Clinical research emphasizes the implementation of rigorous and reproducible study designs that rely on between-group matching or controlling for sources of biological variation such as subject's sex and age. However, corrections for body size (i.e. height and weight) are mostly lacking in clinical neuroimaging designs. This study investigates the importance of body size parameters in their relationship with spinal cord (SC) and brain magnetic resonance imaging (MRI) metrics. Data were derived from a cosmopolitan population of 267 healthy human adults (age 30.1±6.6 years old, 125 females). We show that body height correlated strongly or moderately with brain gray matter (GM) volume, cortical GM volume, total cerebellar volume, brainstem volume, and cross-sectional area (CSA) of cervical SC white matter (CSA-WM; 0.44≤r≤0.62). In comparison, age correlated weakly with cortical GM volume, precentral GM volume, and cortical thickness (-0.21≥r≥-0.27). Body weight correlated weakly with magnetization transfer ratio in the SC WM, dorsal columns, and lateral corticospinal tracts (-0.20≥r≥-0.23). Body weight further correlated weakly with the mean diffusivity derived from diffusion tensor imaging (DTI) in SC WM (r=-0.20) and dorsal columns (-0.21), but only in males. CSA-WM correlated strongly or moderately with brain volumes (0.39≤r≤0.64), and weakly with precentral gyrus thickness and DTI-based fractional anisotropy in SC dorsal columns and SC lateral corticospinal tracts (-0.22≥r≥-0.25). Linear mixture of sex and age explained 26±10% of data variance in brain volumetry and SC CSA. The amount of explained variance increased at 33±11% when body height was added into the mixture model. Age itself explained only 2±2% of such variance. In conclusion, body size is a significant biological variable. Along with sex and age, body size should therefore be included as a mandatory variable in the design of clinical neuroimaging studies examining SC and brain structure., Competing Interests: Declaration of interests Since June 2022, Dr. A.K. Smith has been employed by GE HealthCare. This article was co-authored by Dr. Smith in his personal capacity. The opinions expressed in the article are his in and do not necessarily reflect the views of GE HealthCare. Since August 2022, Dr. M. M. Laganà has been employed by Canon Medical Systems srl, Rome, Italy. This article was co-authored by Dr. M. M. Laganà in her personal capacity. The opinions expressed in the article are her own and do not necessarily reflect the views of Canon Medical Systems. Since September 2023, Dr. Papp has been an employee of Siemens Healthcare AB, Sweden. This article was co-authored by Dr. Papp in his personal capacity. The views and opinions expressed in this article are his own and do not necessarily reflect the views of Siemens Healthcare AB, or Siemens Healthineers AG. Since January 2024, Dr. Barry has been employed by the National Institute of Biomedical Imaging and Bioengineering at the NIH. This article was co-authored by Robert Barry in his personal capacity. The opinions expressed in the article are his own and do not necessarily reflect the views of the NIH, the Department of Health and Human Services, or the United States government. Guillaume Gilbert is an employee of Philips Healthcare. S Llufriu received compensation for consulting services and speaker honoraria from Biogen Idec, Novartis, Bristol Myer Squibb Genzyme, Sanofi Jansen and Merck. The Max Planck Institute for Human Cognitive and Brain Sciences and Wellcome Centre for Human Neuroimaging have institutional research agreements with Siemens Healthcare. NW holds a patent on acquisition of MRI data during spoiler gradients (US 10,401,453 B2). NW was a speaker at an event organized by Siemens Healthcare and was reimbursed for the travel expenses. The other authors declare no competing interests.
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- 2024
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4. BrainWAVE: A Flexible Method for Noninvasive Stimulation of Brain Rhythms across Species.
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Attokaren MK, Jeong N, Blanpain L, Paulson AL, Garza KM, Borron B, Walelign M, Willie J, and Singer AC
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- Humans, Animals, Mice, Cognition, Neurons, Amyloid, Photic Stimulation methods, Brain physiology, Brain Waves physiology
- Abstract
Rhythmic neural activity, which coordinates brain regions and neurons to achieve multiple brain functions, is impaired in many diseases. Despite the therapeutic potential of driving brain rhythms, methods to noninvasively target deep brain regions are limited. Accordingly, we recently introduced a noninvasive stimulation approach using flickering lights and sounds ("flicker"). Flicker drives rhythmic activity in deep and superficial brain regions. Gamma flicker spurs immune function, clears pathogens, and rescues memory performance in mice with amyloid pathology. Here, we present substantial improvements to this approach that is flexible, user-friendly, and generalizable across multiple experimental settings and species. We present novel open-source methods for flicker stimulation across rodents and humans. We demonstrate rapid, cross-species induction of rhythmic activity without behavioral confounds in multiple settings from electrophysiology to neuroimaging. This flicker approach provides an exceptional opportunity to discover the therapeutic effects of brain rhythms across scales and species., Competing Interests: A.C.S. owns shares in Cognito Therapeutics. Her conflict is managed by Georgia Tech. All other authors declare no competing financial interests., (Copyright © 2023 Attokaren et al.)
- Published
- 2023
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5. Goal discrimination in hippocampal nonplace cells when place information is ambiguous.
- Author
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Zhang L, Prince SM, Paulson AL, and Singer AC
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- Action Potentials, Animals, Brain Waves, Cues, Environment, Mice, Pyramidal Cells physiology, Discrimination Learning, Hippocampus cytology, Hippocampus physiology, Spatial Navigation
- Abstract
SignificanceGoal-directed spatial navigation has been found to rely on hippocampal neurons that are spatially modulated. We show that "nonplace" cells without significant spatial modulation play a role in discriminating goals when environmental cues for goals are ambiguous. This nonplace cell activity is performance-dependent and is modulated by gamma oscillations. Finally, nonplace cell goal discrimination coding fails in a mouse model of Alzheimer's disease (AD). Together, these results show that nonplace cell firing can signal unique task-relevant information when spatial information is ambiguous; these signals depend on performance and are absent in a mouse model of AD.
- Published
- 2022
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6. Alzheimer's pathology causes impaired inhibitory connections and reactivation of spatial codes during spatial navigation.
- Author
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Prince SM, Paulson AL, Jeong N, Zhang L, Amigues S, and Singer AC
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- Alzheimer Disease metabolism, Animals, Brain Waves physiology, CA1 Region, Hippocampal metabolism, Disease Models, Animal, Electrodes, Implanted, Humans, Interneurons pathology, Male, Memory physiology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Pyramidal Cells pathology, Synapses pathology, Synaptic Transmission physiology, Virtual Reality, Alzheimer Disease physiopathology, CA1 Region, Hippocampal physiopathology, Interneurons metabolism, Pyramidal Cells metabolism, Spatial Navigation physiology, Synapses metabolism
- Abstract
Synapse loss and altered synaptic strength are thought to underlie cognitive impairment in Alzheimer's disease (AD) by disrupting neural activity essential for memory. While synaptic dysfunction in AD has been well characterized in anesthetized animals and in vitro, it remains unknown how synaptic transmission is altered during behavior. By measuring synaptic efficacy as mice navigate in a virtual reality task, we find deficits in interneuron connection strength onto pyramidal cells in hippocampal CA1 in the 5XFAD mouse model of AD. These inhibitory synaptic deficits are most pronounced during sharp-wave ripples, network oscillations important for memory that require inhibition. Indeed, 5XFAD mice exhibit fewer and shorter sharp-wave ripples with impaired place cell reactivation. By showing inhibitory synaptic dysfunction in 5XFAD mice during spatial navigation behavior and suggesting a synaptic mechanism underlying deficits in network activity essential for memory, this work bridges the gap between synaptic and neural activity deficits in AD., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2021
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7. Multi-sensory Gamma Stimulation Ameliorates Alzheimer's-Associated Pathology and Improves Cognition.
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Martorell AJ, Paulson AL, Suk HJ, Abdurrob F, Drummond GT, Guan W, Young JZ, Kim DN, Kritskiy O, Barker SJ, Mangena V, Prince SM, Brown EN, Chung K, Boyden ES, Singer AC, and Tsai LH
- Subjects
- Alzheimer Disease pathology, Amyloid metabolism, Amyloid beta-Peptides metabolism, Animals, Auditory Perception physiology, Brain metabolism, Disease Models, Animal, Gamma Rhythm physiology, Hippocampus metabolism, Male, Mice, Mice, Inbred C57BL, Microglia metabolism, Plaque, Amyloid metabolism, Acoustic Stimulation methods, Alzheimer Disease therapy, Cognition physiology
- Abstract
We previously reported that inducing gamma oscillations with a non-invasive light flicker (gamma entrainment using sensory stimulus or GENUS) impacted pathology in the visual cortex of Alzheimer's disease mouse models. Here, we designed auditory tone stimulation that drove gamma frequency neural activity in auditory cortex (AC) and hippocampal CA1. Seven days of auditory GENUS improved spatial and recognition memory and reduced amyloid in AC and hippocampus of 5XFAD mice. Changes in activation responses were evident in microglia, astrocytes, and vasculature. Auditory GENUS also reduced phosphorylated tau in the P301S tauopathy model. Furthermore, combined auditory and visual GENUS, but not either alone, produced microglial-clustering responses, and decreased amyloid in medial prefrontal cortex. Whole brain analysis using SHIELD revealed widespread reduction of amyloid plaques throughout neocortex after multi-sensory GENUS. Thus, GENUS can be achieved through multiple sensory modalities with wide-ranging effects across multiple brain areas to improve cognitive function., (Copyright © 2019 Elsevier Inc. All rights reserved.)
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- 2019
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8. Determining iron oxide nanoparticle heating efficiency and elucidating local nanoparticle temperature for application in agarose gel-based tumor model.
- Author
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Shah RR, Dombrowsky AR, Paulson AL, Johnson MP, Nikles DE, and Brazel CS
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- Animals, Humans, Polyethylene Glycols chemistry, Polyethylene Glycols pharmacology, Sepharose chemistry, Ferric Compounds chemistry, Ferric Compounds pharmacology, Hyperthermia, Induced methods, Magnetic Field Therapy methods, Models, Biological, Nanoparticles chemistry, Neoplasms therapy
- Abstract
Magnetic iron oxide nanoparticles (MNPs) have been developed for magnetic fluid hyperthermia (MFH) cancer therapy, where cancer cells are treated through the heat generated by application of a high frequency magnetic field. This heat has also been proposed as a mechanism to trigger release of chemotherapy agents. In each of these cases, MNPs with optimal heating performance can be used to maximize therapeutic effect while minimizing the required dosage of MNPs. In this study, the heating efficiencies (or specific absorption rate, SAR) of two types of MNPs were evaluated experimentally and then predicted from their magnetic properties. MNPs were also incorporated in the core of poly(ethylene glycol-b-caprolactone) micelles, co-localized with rhodamine B fluorescent dye attached to polycaprolactone to monitor local, nanoscale temperatures during magnetic heating. Despite a relatively high SAR produced by these MNPs, no significant temperature rise beyond that observed in the bulk solution was measured by fluorescence in the core of the magnetic micelles. MNPs were also incorporated into a macro-scale agarose gel system that mimicked a tumor targeted by MNPs and surrounded by healthy tissues. The agarose-based tumor models showed that targeted MNPs can reach hyperthermia temperatures inside a tumor with a sufficient MNP concentration, while causing minimal temperature rise in the healthy tissue surrounding the tumor., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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9. A Narrative Review of Pharmacologic and Non-pharmacologic Interventions for Disorders of Consciousness Following Brain Injury in the Pediatric Population.
- Author
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Evanson NK, Paulson AL, and Kurowski BG
- Abstract
Traumatic brain injury (TBI) is the most common cause of long-term disability in the United States. A significant proportion of children who experience a TBI will have moderate or severe injuries, which includes a period of decreased responsiveness. Both pharmacological and non-pharmacological modalities are used for treating disorders of consciousness after TBI in children. However, the evidence supporting the use of potential therapies is relatively scant, even in adults, and overall, there is a paucity of study in pediatrics. The goal of this review is to describe the state of the science for use of pharmacologic and non-pharmacologic interventions for disorders of consciousness in the pediatric population.
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- 2016
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10. Immunogenicity and reactivity of novel Mycobacterium avium subsp. paratuberculosis PPE MAP1152 and conserved MAP1156 proteins with sera from experimentally and naturally infected animals.
- Author
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Bannantine JP, Paulson AL, Chacon O, Fenton RJ, Zinniel DK, McVey DS, Smith DR, Czuprynski CJ, and Barletta RG
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- Acyltransferases genetics, Acyltransferases immunology, Acyltransferases metabolism, Animals, Antibodies, Bacterial blood, Antigens, Bacterial genetics, Antigens, Bacterial immunology, Bacterial Proteins genetics, Cattle, Cattle Diseases immunology, Cattle Diseases microbiology, Diglycerides metabolism, Mice, Mycobacterium avium subsp. paratuberculosis genetics, Paratuberculosis microbiology, Rabbits, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins immunology, Bacterial Proteins immunology, Immune Sera immunology, Mycobacterium avium subsp. paratuberculosis immunology, Paratuberculosis immunology
- Abstract
Mycobacterium avium subsp. paratuberculosis causes Johne's disease (JD) in ruminants. Development of genetic tools and completion of the M. avium subsp. paratuberculosis genome sequencing project have expanded the opportunities for antigen discovery. In this study, we determined the seroreactivities of two proteins encoded at the 5' and 3' regions of the MAP1152-MAP1156 gene cluster. MAP1152 encodes a PPE protein, and MAP1156 encodes a diacylglycerol acyltransferase involved in triglyceride metabolism and classified in the uncharacterized protein family UPF0089. Recombinant MAP proteins were overproduced and purified from Escherichia coli as maltose-binding protein (MBP) fusions. Immunoblotting analysis indicated that both MAP1152 and MAP1156 displayed reactivity against sera of mice and rabbits immunized with live M. avium subsp. paratuberculosis cells and against samples from naturally infected cattle. In immunoblot assays, MAP1156 yielded a stronger positive signal than MAP1152 against sera from cattle with JD. An enzyme-linked immunosorbent assay for the recombinant proteins was developed and used to test preclassified positive and negative serum samples from naturally infected and noninfected cattle. Samples, with one exception, displayed no seroreactivity against the MBP-LacZ fusion protein (P > 0.05), the negative-control antigen. MAP1152 displayed seroreactivity against all positive sera but no seroreactivity to the negative sera (P < 0.01). MAP1156 displayed stronger and more variable reactivity than MAP1152, but significant differences were observed between noninfected and infected cattle (P < 0.05). Otherwise, degrees of reactivity followed the same trend as the positive reference antigen. In conclusion, both proteins are immunogenic in mice and rabbits, and M. avium subsp. paratuberculosis-infected cattle mount a humoral response to both MAP1152 and MAP1156 cross-reactive epitopes. These findings have potential applications to diagnostics, vaccine production, and elucidation of the immunopathogenesis of JD.
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- 2011
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11. Endopeptidase penicillin-binding proteins 4 and 7 play auxiliary roles in determining uniform morphology of Escherichia coli.
- Author
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Meberg BM, Paulson AL, Priyadarshini R, and Young KD
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- Endopeptidases genetics, Escherichia coli genetics, Escherichia coli metabolism, Escherichia coli Proteins genetics, Flow Cytometry, Gene Deletion, Penicillin-Binding Proteins genetics, Endopeptidases metabolism, Escherichia coli physiology, Escherichia coli Proteins metabolism, Gene Expression Regulation, Bacterial, Penicillin-Binding Proteins metabolism
- Abstract
The low-molecular-weight (LMW) penicillin-binding protein, PBP 5, plays a dominant role in determining the uniform cell shape of Escherichia coli. However, the physiological functions of six other LMW PBPs are unknown, even though the existence and enzymatic activities of four of these were established three decades ago. By applying fluorescence-activated cell sorting (FACS) to quantify the cellular dimensions of multiple PBP mutants, we found that the endopeptidases PBP 4 and PBP 7 also influence cell shape in concert with PBP 5. This is the first reported biological function for these two proteins. In addition, the combined loss of three DD-carboxypeptidases, PBPs 5 and 6 and DacD, also impaired cell shape. In contrast to previous reports based on visual inspection alone, FACS analysis revealed aberrant morphology in a mutant lacking only PBP 5, a phenotype not shared by any other strain lacking a single LMW PBP. PBP 5 removes the terminal D-alanine from pentapeptide side chains of muropeptide subunits, and pentapeptides act as donors for cross-linking adjacent side chains. As endopeptidases, PBPs 4 and 7 cleave cross-links in the cell wall. Therefore, overall cell shape may be determined by the existence or location of a specific type of peptide cross-link, with PBP 5 activity influencing how many cross-links are made and PBPs 4 and 7 acting as editing enzymes to remove inappropriate cross-links.
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- 2004
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12. Contribution of membrane-binding and enzymatic domains of penicillin binding protein 5 to maintenance of uniform cellular morphology of Escherichia coli.
- Author
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Nelson DE, Ghosh AS, Paulson AL, and Young KD
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- Amino Acid Sequence, Base Sequence, Binding Sites, Carrier Proteins genetics, Endopeptidases metabolism, Molecular Sequence Data, Muramoylpentapeptide Carboxypeptidase genetics, Mutagenesis, Site-Directed, Penicillin-Binding Proteins, Polymerase Chain Reaction methods, Protein Structure, Tertiary, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Sequence Homology, Amino Acid, Bacterial Proteins, Carrier Proteins metabolism, Cell Membrane metabolism, Dipeptidases, Escherichia coli physiology, Hexosyltransferases, Muramoylpentapeptide Carboxypeptidase metabolism, Peptidyl Transferases
- Abstract
Four low-molecular-weight penicillin binding proteins (LMW PBPs) of Escherichia coli are closely related and have similar DD-carboxypeptidase activities (PBPs 4, 5, and 6 and DacD). However, only one, PBP 5, has a demonstrated physiological function. In its absence, certain mutants of E. coli have altered diameters and lose their uniform outer contour, resulting in morphologically aberrant cells. To determine what differentiates the activities of these LMW PBPs, we constructed fusion proteins combining portions of PBP 5 with fragments of other DD-carboxypeptidases to see which hybrids restored normal morphology to a strain lacking PBP 5. Functional complementation occurred when truncated PBP 5 was combined with the terminal membrane anchor sequences of PBP 6 or DacD. However, complementation was not restored by the putative carboxy-terminal anchor of PBP 4 or by a transmembrane region of the osmosensor protein ProW, even though these hybrids were membrane bound. Site-directed mutagenesis of the carboxy terminus of PBP 5 indicated that complementation required a generalized amphipathic membrane anchor but that no specific residues in this region seemed to be required. A functional fusion protein was produced by combining the N-terminal enzymatic domain of PBP 5 with the C-terminal beta-sheet domain of PBP 6. In contrast, the opposite hybrid of PBP 6 to PBP 5 was not functional. The results suggest that the mode of PBP 5 membrane anchoring is important, that the mechanism entails more than a simple mechanical tethering of the enzyme to the outer face of the inner membrane, and that the physiological differences among the LMW PBPs arise from structural differences in the DD-carboxypeptidase enzymatic core.
- Published
- 2002
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