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Molecular docking is a widely used technique in drug discovery to predict the binding mode of a given ligand to its target. However, the identification of the near-native binding pose in docking experiments still represents a challenging task as the scoring functions currently employed by docking programs are parametrized to predict the binding affinity, and, therefore, they often fail to correctly identify the ligand native binding conformation. Selecting the correct binding mode is crucial to obtaining meaningful results and to conveniently optimizing new hit compounds. Deep learning (DL) algorithms have been an area of a growing interest in this sense for their capability to extract the relevant information directly from the protein-ligand structure. Our review aims to present the recent advances regarding the development of DL-based pose selection approaches, discussing limitations and possible future directions. Moreover, a comparison between the performances of some classical scoring functions and DL-based methods concerning their ability to select the correct binding mode is reported. In this regard, two novel DL-based pose selectors developed by us are presented.

River contamination by microorganisms, or another chemical source, poses a serious threat to both the environment and public health. Taking immediate and appropriate actions is essential to mitigate the contamination and prevent further spread. As such, regular monitoring of these pollution agents is essential to act in time and control its minor extension. However, there is a lack of commitment to this emergent concern and respective actions around the world. This work aims to study the contamination of a Portuguese river (Tinto River) within Porto city (a highly populated urban area) regarding the total aerobic microorganisms, coliforms, and Enterococcus (as colony-forming units (CFUs) using specific solid culture media) and total organic matter (TOC). Different locations were considered along the Tinto River course (i.e., 14 locations within 11 km) and samples were collected on distinct days throughout September 2022. The overall results showed microbial contamination of aerobic microorganisms (up to 2 × 105 CFU/100 mL), total coliforms (up to 7 × 104 CFU/100 mL), Escherichia coli (up to 9 × 103 CFU/100 mL), and Enterococcus (up to 8 × 103 CFU/100 mL). The results also surpassed the maximum recommended values (MRVs) described in Portuguese decree-law no. 236/98 for irrigation waters. Moreover, TOC was found in a range of 4.54 mg/L to 57.2 mg/L. This work highlights the dangerous microbial contamination and higher amount of organic matter than would be expected for a surface water resource.

Machine learning-based systems have presented increasing learning performance, in a wide variety of tasks. However, the problem with some state-of-the-art models is their lack of transparency, trustworthiness, and explainability. To address this problem, eXplainable Artificial Intelligence (XAI) appeared. It is a research field that aims to make black-box models more understandable to humans. The research on this topic has increased in recent years, and many methods, such as LIME (Local Interpretable Model-Agnostic Explanations) and SHAP (SHapley Additive exPlanations) have been proposed. Machine learning-based Intrusion Detection Systems (IDS) are one of the many application domains of XAI. However, most of the works about model interpretation focus on other fields, like computer vision, natural language processing, biology, healthcare, etc. This poses a challenge for cybersecurity professionals tasked with analyzing IDS results, thereby impeding their capacity to make informed decisions. In an attempt to address this problem, we have selected two XAI methods, LIME, and SHAP. Using the methods, we have retrieved explanations for the results of a black-box model, part of an IDS solution that performs intrusion detection on IoT devices, increasing its interpretability. In order to validate the explanations, we carried out a perturbation analysis where we tried to obtain a different classification based on the features present in the explanations. With the explanations and the perturbation analysis we were able to draw conclusions about the negative impact of particular features on the model results when present in the input data, making it easier for cybersecurity experts when analyzing the model results and it serves as an aid to the continuous improvement the model. The perturbations also serve as a comparison of performance between LIME and SHAP. To evaluate the degree of interpretability increase, and the explanations provided by each XAI method of the model and directly compare the XAI methods, we have performed a survey analysis.

Women in menopause have more mood swings than before menopause. Several studies attempt to clarify the role of menopause in the development of depression, anxiety, and stress in midlife. However, there is controversy about the relationship of body composition with psychological distress in postmenopausal women. This study investigated the relationship between fat mass (FM) and visceral fat level (FVL) with anxiety, depression and stress in women with menopause for at least 1 year. The study included 50 women (57,49±5.62 years), 46% for more than 6 years in estrogen depletion. Women who documented the use of antidepressants or anxiolytics were excluded from the analysis. The short-form version of the Depression Anxiety and Stress Scale (DASS-21) was administered, and body composition (FM, VFL, SMM - skeletal muscle mass) was assessed with the bioimpedance InBody 120. High levels of total and central adiposity were considered for %FM≥35% (Lohman & Going, 1998) and VFL>9 (Biospace, 2021). Spearman's correlation coefficient was used to analyse the association of variables. Comparisons between groups were conducted using non-parametric tests, based on the normality test results, and a statistical significance was set at p< 0.05. The largest part of the sample documented a natural menopause (86%) and the absence of hormone therapy. The mean DASS-21 scale was 10.56 (±8.83), with higher levels for stress (4.72±3.77) and depression (3.56±3.81). All women exhibited normal muscle condition (SMM≥16 kg), but most of them had obesity (68%), especially central obesity (76%). A significant association (p=0.04) was identified between %FM and the DASS-21 scale (r=0.20, p=0.04). Obese women had lower stress (p=0.04) but a higher value on the DASS-21 scale (p=0.04). In parallel, the presence of greater levels of central adiposity determined higher values of these two measures of psychological distress (p=0.01). The results suggest that postmenopausal women with higher levels of fat mass or central adiposity tend to have a higher DASS-21 scale score. Central obesity produces in women symptoms such as difficulty in relaxing, irritability, and nervous excitement.

Introdução: A infeção protésica é das complicações mais temíveis no decorrer de uma artroplastia e, dada a sua natureza multidisciplinar, a abordagem clínica permanece um desafio. Assim, é fundamental a cooperação entre especialidades médicas e cirúrgicas, destacando que um diagnóstico microbiológico o mais preciso possível é crucial para um tratamento efetivo (McNally M et al., 2021). Objetivos: Caracterizar as Infeções Osteoarticulares Associadas a Prótese (IOAP) em doentes do CHUdSA, designadamente analisar o perfil microbiológico, avaliar o perfil de suscetibilidade antimicrobiana dos microrganismos isolados e averiguar os locais anatómicos mais afetados nestas infeções. Material e Métodos: Realizou-se um estudo descritivo transversal recorrendo a uma amostra de registos de IOAP de doentes do CHUdSA, entre 2016 e 2022. Resultados: Foram incluídos 189 casos de IOAP (anca: n = 97; joelho: n = 85; ombro: n = 7). Os microrganismos mais frequentes foram Staphylococcus coagulase-negativo (SCoN) (n = 205, 46,91%), dos quais 56,59% (n = 116) correspondem a Staphylococcus epidermidis, seguido pelo Staphylococcus aureus (n = 44, 10,07%) e Streptococcus (n = 28, 6,41%). Bactérias Gram-negativo e fungos foram detetados em 21,28% (n = 93) e 1,83% (n = 8) de todos os casos, respetivamente. 41,49% dos Staphylococcus apresentaram resistência à oxacilina, dos quais 70,83% correspondem a S.epidermidis e 11,46% a S.aureus. Em relação à Pseudomonas, 52,94% apresentaram resistência à ciprofloxacina. Conclusões: A maioria dos agentes patogénicos isolados neste estudo corresponde a SCoN. Dos antibióticos testados, a oxacilina foi o que apresentou maior frequência de resistência, conferida, sobretudo, pelo S.epidermidis. O MRSE é, atualmente, uma das problemáticas centrais das IOAP, acarretando elevada incidência de infeções persistentes (Rosteius T et al., 2018). A resistência à ciprofloxacina é preocupante pelo impacto nevrálgico na indução de outras resistências, pelo que a sua redução é um dos objetivos do PPCIRA (DGS, 2021). O microambiente ósseo e a sua dinâmica fazem das IOAP um objeto de estudo complexo (McNally M et al., 2021). Futuramente, propõe-se a realização de estudos que avaliem outras variáveis como comorbilidades, material protésico e tipologia de infeção, a fim de se obter maior informação sobre o seu impacto na patogénese destas infeções.

Train stops on the Portuguese state network are a reference to the country's heritage. Their architecture is an invitation in many cities and towns not only to make a journey, but also to understand the territory and the cities where they are located. Many of these spaces can be the first stop of a tourist visit and a space of reflection to understand the evolution of the nearby geographical space and of the one further away. This methodological proposal offers a reading where the landscapes and cultural elements represented in their tile panels are identified, catalogued and interpreted from the perspective and understanding of railway architecture and the designs and forms of artistic expression used for the representation of Portuguese landscapes. For this research, the urban train network of the city of Porto (Portugal) was used due to its quantity and variety of elements for its study.

No Brasil, ainda há poucos estudos relacionados à utilização de softwares em Realidade Virtual (RV) como adjuvantes no tratamento de Transtornos Mentais. Além disso, o campo das Adições carece de novas técnicas que possam aumentar a efetividade das técnicas e abordagens terapêuticas, em função do baixo índice de adesão e altas taxas de recaída e abandono por parte dos pacientes. O presente estudo teve por objetivo adaptar a Terapia de Exposição a Pistas Ambientais em Realidade Virtual (TEPARV) para o contexto brasileiro através de uma revisão narrativa, desenvolvimento de um manual para a cultura brasileira e teste usando um software com uma amostra preliminar de 7 pacientes com Transtorno por Uso de Álcool (TUA), em regime de internação hospitalar. Para os testes com os pacientes foi utilizado o dispositivo Oculus Quest 1 e o software da empresa francesa C2 CARE específico para TUA. vsubmetidos ao teste do software com os pacientes com a técnica depressivos, ansiosos, senso de presença em ambiente virtual. Possíveis limitações associadas à sessão podem ter relação à amostra pequena, à versão com som mais básico desta versão do software, ao software não apresentar ambientes virtuais com pessoas ingerindo álcool para o Oculus Quest 1 usado no teste, ao número de sessões realizadas e ao conhecimento por parte da amostra sobre RV, sendo que a maioria dos estudos utilizados durante a pesquisa apresentaram a fissura como resultado da sessão.

It is known that inflammation worsen the course of schizophrenia and induce high clozapine serum levels. However, no study evaluated this change in function of clozapine daily dose in schizophrenia. We assessed the correlation between inflammation and severity symptoms in patients with schizophrenia that take and do not take clozapine. We also assessed the correlation between clozapine daily dose and inflammatory markers to patients who take this drug. Patients were recruited from Schizophrenia Ambulatory and Psychosocial Care Center of Clinical Hospital of Porto Alegre and from an association of relatives of patients with schizophrenia. Exam results, and other important clinical exam were assessed in patients record or patients were asked to show their exam in the case of outpatients. We included 104 patients, 90 clozapine users and 14 non-clozapine users. We calculate the systemic inflammatory markers [neutrophil-lymphocyte ratio (NLR), systemic immune inflammation index (SII), and the psychopathology severity by the Brief Psychiatric Rating Scaled anchored (BPRS-a)]. These variables were compared between clozapine users and non-clozapine users. It was used mean/median test according to data distributing, with study factor (SII, MLR, and PLR), the clinical outcome: severity of symptomatology (BPRS score), and clozapine daily dose as adjustment factor. Clozapine users exhibited a significantly higher neutrophil count (mean ± SD: 5.03 ± 2.07) compared to non-clozapine users (mean ± SD: 3.48 ± 1.27; p = 0.031). After controlling for comorbidity, other parameters also showed significant differences. These findings are consistent with previous studies that have demonstrated an inflammatory response following the administration of clozapine.

ABSTRACT Background: Here, Leishmania presence in sand flies from Três Lagoas, Mato Grosso do Sul, Brazil, after visceral leishmaniasis (VL) was investigated. Methods: In April 2022, two light traps were deployed within and around the residence for two days post-VL case report. Results: A total of 120 Lutzomyia longipalpis were collected. Suprapyloric flagellates were found in a female sand fly with eggs and residual blood during midgut dissection. Sequencing of ITS1 and cytb fragments confirmed Leishmania infantum DNA and identified Homo sapiens as the blood source, respectively. Conclusions: This study emphasizes the importance of monitoring sand flies in VL endemic areas.

No mês de março de 2020, a Organização Mundial da Saúde (OMS) declarou estado de pandemia, em decorrência do vírus SARS-CoV-2/COVID-19. No início de 2021 foram registrados 83.715.617 casos confirmados e 1.835.901 mortes por COVID-19. Desse modo, mundialmente há um esforço no sentido de desenvolver protocolos eficazes para o tratamento dessa doença. Uma das dificuldades encontradas é devida à heterogeneidade com o qual a COVID-19 afeta diferentes pacientes, devido a comorbidades. A não existência de medicamentos específicos no tratamento da COVID-19 é preocupante. Esse quadro se torna ainda mais grave quando consideramos possíveis interações entre esses medicamentos e os já utilizados previamente pelos pacientes, principalmente os de uso crônico. Nesse contexto, se enquadram os pacientes que possuam transtornos psiquiátricos, como transtorno depressivo maior, transtorno afetivo bipolar e esquizofrenia. Essa revisão teve como objetivo comparar diferentes protocolos farmacoterapêuticos no tratamento da COVID-19 em diferentes estágios quanto à sua segurança e eficácia em pacientes em tratamento para depressão, transtorno bipolar e esquizofrenia. Foi feita uma revisão em uma base de dados acerca de medicamentos mais citados para COVID-19 e pesquisadas possíveis interações entre os cinco mais citados no tratamento da COVID-19 e protocolos no tratamento de esquizofrenia, transtorno depressivo maior e transtorno afetivo bipolar. Foram excluídos ensaios não-clínicos, artigos que não apresentassem resultados (trials), artigos que apresentaram posterior retratação, artigos com idiomas estranhos aos autores e artigos relacionados a vacinas e terapias imunológicas que estão fora do escopo desse trabalho. Após as exclusões, foram considerados 117 estudos. Os medicamentos mais citados foram Hidroxicloroquina/Cloroquina (26 artigos), seguido por tocilizumab (10 artigos), ivermectina e rendesivir (oito artigos cada) e flavipinavir (sete artigos) A hidroxicloroquina e a cloroquina apresentaram vários resultados conflitantes e foram os medicamentos mais associados com interações medicamentosas em todas as classes de medicamentos estudadas.

Francisco Botelho de Morais e Vasconcelos (1670-1747) es una importante figura de las letras ibéricas de las últimas décadas del siglo XVII y primeras del XVIII. Escribió en español buena parte de sus obras literarias, incluyendo dos poemas épicos, El Nuevo Mundo y El Alphonso, o la fundación del reino de Portugal, asegurada y perfecta en la conquista de Lisboa. En este estudio vamos a analizar este último y su compleja relación con las epopeyas de la Edad Moderna, sin olvidar la definición y características del poema épico. Seguramente el apogeo de este género fue en la segunda mitad del XVI y primeras décadas del XVII, pero otros autores han ido creando nuevas formulaciones a partir de dinámicas de contaminación con distintos modelos literarios. Siendo producida en la época de entre siglos, esta obra cobra un significado especial para medir el cambio de mentalidad en la transición del Barroco a la Ilustración. En la edición de El Alphonso (Salamanca, 1731), el autor se sirve de la recuperación de las tierras peninsulares de manos de los invasores árabes como momento que daba paso a la edificación de un imperio cristiano universal, a la luz de una concepción mesiánica y providencialista de la Historia. Por otro lado, Botelho se manifiesta abierto al influjo de la cultura moderna y de las nuevas teorías científicas, pero sin cuestionar el respecto por los dogmas de la religión.

Este estudo trata da observação de um caso de aplicação dos princípios de educação inovadora no treinamento de Condução Veicular Policial (CVP), aplicado durante o Ciclo de Atualização Policial da Polícia Rodoviária Federal (PRF), evento de capacitação profissional realizado anualmente por Policiais Rodoviários Federais como forma de aprimoramento e atualização das técnicas necessárias para o exercício de suas funções. Por meio do emprego de técnicas pedagógicas, como a gamificação, buscou-se induzir nos instruendos o estado de flow na prática de exercícios que simulam situações que poderão ser encontradas nas rotinas operacionais diárias, tendo como objetivo o desenvolvimento de práticas de direção segura para a redução dos índices de vitimização policial por acidentes de trânsito. Os resultados observados apontam para um elevado nível de comprometimento dos instruendos e revelam a importância do treinamento de CVP no âmbito da educação corporativa da PRF.

Flue gas desulfurization (FGD) waste is an industrial by-product generated during the flue gas desulfurization process in coal-fired power plants. This by-product contain trace quantities of naturally occurring radionuclides and elements such as As, Ba, Co, Cr, Zn. The characteristics of FGD waste are important for its reuse and are mainly depend on the desulfurization process. In this work, two types of FGD materials collected from three coal-fired power plants using semi-dry and wet processes were characterized by X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), X-ray fluorescence (XFR) and particle size analysis. The radioactive content of 238U, 232Th, 228Th, 226Ra, 228Ra, 210Pb and 40K and trace elements were also determined using Neutron activation analysis and Gamma-ray spectrometry. The major constituents for all samples were Ca, Si, S, Al and Fe. Wet FGD by-product presented also high magnesium content. The wastes contain mainly semi-hydrate calcium sulfite and calcium sulfate. The particle size of FGD from semi-dry process was lower than that from the wet process. The average activity concentration of 238U, 232Th, 226Ra, 210Pb, 228Ra, 228Th and 40K varied between were 50-71, 33-42, 28-52, 113-150, 26-33, 33-39, 161-390 Bq kg-1, respectively. According to the results of leaching and solubilization tests, FGD samples were classified as non-hazardous and non-inert. The obtained data are useful for evaluation of possible applications of FGD by-products.

Abstract Background In the State of Mato Grosso do Sul, Brazil, sand flies and cases of visceral (VL) and cutaneous (CL) leishmaniases have been reported in almost all municipalities. The aim of this study was to analyze the geographic distribution of VL and CL in relation the sand fly species found in the municipalities of Mato Grosso do Sul. Methods We analyzed VL and CL cases from 2001 to 2018 using data from the Notifiable Diseases Information System (SINAN). Data collected since 2003 on the presence of sand fly vectors (proven or suspected) were provided by the State Health Secretariat. Results A total of 3566 and 3030 cases of VL and CL, respectively, were reported from 2001 to 2018. The municipalities with the most reported cases of VL were Campo Grande (2495), Três Lagoas (442), Corumbá (140) and Aquidauana (136); and those for CL were Campo Grande (635) and Bodoquena (197). The following sand fly species with vector potential were found in 59 municipalities (74.7%): Lutzomyia longipalpis, Lutzomyia cruzi, Nyssomyia whitmani, Migonemyia migonei, Nyssomyia neivai, Pintomyia pessoai, Bichromomyia flaviscutellata and Pintomyia fischeri. Sand flies were present in six municipalities where no cases of VL were reported and in two municipalities where no cases of CL were reported. Conclusions Our results indicate that the geographical distribution of VL and CL in Mato Grosso do Sul expanded during the study period, and highlight the presence of sand fly vectors in municipalities where these diseases are currently considered to be non-endemic. Graphical Abstract

Numa altura em que se celebram os 450 anos da publicação de Os Lusíadas, pretende-se revisitar a épica camoniana e a sua presença assídua em debates intelectuais dos séculos XVIII e XIX, para além do seu contributo para a afirmação da cultura de língua portuguesa. Para isso, analisaremos testemunhos em que a discussão em torno do(s) modelo(s) da épica se cruza com opiniões veiculadas por Voltaire ou com a apropriação de textos voltairianos por parte de comentadores. O Ensaio Sobre a Poesia Épica, que marca um período em que o autor começava a percorrer os domínios da filosofia e da história, mas sem abandonar a sua atuação como poeta, apresenta material riquíssimo. A primeira versão surgiu durante o exílio na Inglaterra, com o objetivo de preparar o caminho para a publicação de La Henriade. Seguem-se as polémicas, traduções não autorizadas e diferentes versões até que, em 1732, publica a sua própria versão (revista) em francês. Voltaire selecionou oito estudos de caso, enumerando e comparando virtudes e defeitos de cada um deles: Poemas Homéricos, Virgílio, Lucano, Tasso, Trissino, Ercilla, Camões e Milton. No caso de Camões, as críticas devem-se ao facto de misturar divindades clássicas com entidades religiosas e ao uso de ornato estilístico desnecessário. Quanto à Henriade, poema que contou com traduções para português logo a partir do séc. XVIII, sabe-se que influenciou a perceção sobre o que (e como) deveria ser a poesia épica, pelo que não causa estranheza a sua inclusão nestes debates. Do corpus fazem parte textos de F. Xavier de Meneses, J. Agostinho de Macedo, William Julius Mickle, Tomás José de Aquino, J. A. Bezerra de Lima, Morgado de Mateus, entre outros.

O presente trabalho tem como objetivo analisar sobre a abordagem do pensamento algébrico nos conteúdos de Álgebra apresentados nos Currículos disponíveis ao longo do tempo, em especial, aqueles adotados pelo estado de São Paulo no segmento do Ensino Fundamental nos Anos Finais. Em seguida, apresentamos os estudos de alguns autores que discorrem sobre o pensamento algébrico que justificam a pertinência da pesquisa, como, Blanton e Kaput (2005), Ponte, Branco e Matos (2009), Lima e Bianchini (2017), Gomes e Noronha (2020), Lins e Gimenez (2001). Para isso foi desenvolvida uma pesquisa de caráter qualitativo com análise dos documentos curriculares disponíveis no Brasil ao longo do tempo, dentre eles, os Guias Curriculares (1975), a Proposta Curricular de Matemática (1992), os Parâmetros Curriculares Nacionais - PCN (1998), a Base Nacional Comum Curricular - BNCC (2017) e o Currículo Paulista (2019). Nos resultados esperados consideramos que cada vez mais se torna eficaz para o entendimento do processo ensino aprendizagem da Álgebra, os estudos e o acompanhamento da manifestação do pensamento algébrico dos estudantes em sala de aula, assim como, o acompanhamento de suas respectivas aprendizagens em prol de documentos curriculares coesos e que buscam entender o que os alunos aprenderam ao sair da escola.

O presente trabalho tem como objetivo analisar os principais impactos ocasionados pela implementação das normas internacionais (IFRS) para o cálculo da PECLD – Provisão para Perdas Estimadas com Crédito de Liquidação Duvidosa no percentual de provisão das operações de crédito das instituições financeiras que atuam no Brasil. O referencial teórico aborda os conceitos necessários para o entendimento acerca de crédito e provisão para crédito de liquidação duvidosa. Além disso, são expostos os requerimentos mínimos da Resolução CMN 2.682/99 bem como os requerimentos mínimos da IFRS 9. Para analisar o impacto mencionado, foram analisadas três instituições financeiras que atuam no Brasil, e para tal análise tabulou-se os dados da carteira de crédito (exposição) bem como da provisão nos sites de tais instituições. Por meio de uma metodologia descritiva, comparou-se o percentual de cobertura da provisão em relação à exposição da carteira de crédito dos dados publicados em IFRS e BRGAAP. Observou-se que todas as instituições financeiras analisadas apresentaram exposição e provisão de crédito de liquidação duvidosa na publicação IFRS superior a publicação segundo o BRGAAP (PECLD mínima), conforme expectativa.

Aedes (Ochlerotatus) lepidus (Cerqueira & Paraense, 1945) is reported from a cave in Templo dos Pilares Municipal Park, Mato Grosso do Sul state, Brazil. A single exploratory collection of culicids was carried out using CDC light traps on 16 November 2015. Four larvae and 11 adults (1♂, 10♀) were collected. The larvae were foundin a water-filled hole in a stone. This is the first record of the species in Mato Grosso do Sul, which increases to 63 the number of culicids species recorded in the state.

Private data is transmitted and stored online every second. Therefore, security and privacy assurances should be provided at all times. However, that is not always the case. Private information is often unwillingly collected, sold, or exposed, depriving data owners of their rightful privacy. In this article, various privacy threats, concepts, regulations, and personal data types are analyzed. An overview of Privacy Enhancing Technologies (PETs) and a survey of anonymization mechanisms, privacy tools, models, and metrics are presented together with an analysis of respective characteristics and capabilities. Moreover, this article analyses the applicability of the reviewed privacy mechanisms on today's Cloud Services and identifies the current research challenges to achieve higher privacy levels in the Cloud.

Machado-Joseph disease (MJD/SCA3) is a neurodegenerative polyglutamine disorder exhibiting a wide spectrum of phenotypes. The abnormal size of the (CAG)n at ATXN3 explains ~55% of the age at onset variance, suggesting the involvement of other factors, namely genetic modifiers, whose identification remains limited. Our aim was to find novel genetic modifiers, analyse their epistatic effects and identify disease-modifying pathways contributing to MJD variable expressivity. We performed whole-exome sequencing in a discovery sample of four age at onset concordant and four discordant first-degree relative pairs of Azorean patients, to identify candidate variants which genotypes differed for each discordant pair but were shared in each concordant pair. Variants identified by this approach were then tested in an independent multi-origin cohort of 282 MJD patients. Whole-exome sequencing identified 233 candidate variants, from which 82 variants in 53 genes were prioritized for downstream analysis. Eighteen disease-modifying pathways were identified; two of the most enriched pathways were relevant for the nervous system, namely the neuregulin signaling and the agrin interactions at neuromuscular junction. Variants at PARD3, NFKB1, CHD5, ACTG1, CFAP57, DLGAP2, ITGB1, DIDO1 and CERS4 modulate age at onset in MJD, with those identified in CFAP57, ACTG1 and DIDO1 showing consistent effects across cohorts of different geographical origins. Network analyses of the nine novel MJD modifiers highlighted several important molecular interactions, including genes/proteins previously related with MJD pathogenesis, namely between ACTG1/APOE and VCP/ITGB1. We describe novel pathways, modifiers, and their interaction partners, providing a broad molecular portrait of age at onset modulation to be further exploited as new disease-modifying targets for MJD and related diseases.

Introdução: Avaliar a associação entre níveis plasmáticos da quimiocina CCL11, coeficiente de inteligência e prática da amamentação em homens com esquizofrenia em condições psiquiátricas estáveis sob acompanhamento ambulatorial em um serviço de saúde pública. Métodos: Foi realizado estudo caso-controle com 60 indivíduos: 30 pacientes com esquizofrenia e 30 controles saudáveis, dos quais 15 de cada grupo foram expostos ao aleitamento materno e 15 não foram. Foi aplicado questionário abordando questões socioeconômicas, história ao nascer, dados clínicos e alimentação ao nascer. Foi dosada a quimiocina CCL11 e aplicados testes psicológicos para avaliar quociente de inteligência, funcionalidade, sintomas psiquiátricos, curso da doença e diagnóstico. Para os controles, foi utilizada uma escala para descartar doença psiquiátrica. Resultados: A quimiocina CCL11 apresentou valores significativamente mais altos (> 0,5) em pacientes com esquizofrenia quando comparados aos controles. No grupo de amamentados, os esquizofrênicos apresentaram valores significativamente mais altos a nível intermediário (entre 0.106 e 0.5). Não houve correlação da CCL11 com o número de hospitalizações, idade, tempo de diagnóstico e escolaridade. Não foi evidenciada correlação entre tempo de aleitamento materno em relação aos fatores do Brief Psychiatric Rating Scale. Houve uma tendência de correlação entre a idade de início da doença e o aleitamento materno. Foi encontrada correlação positiva do CCL11 com o tempo de aleitamento materno. Ao comparar os pacientes esquizofrênicos que foram aleitados com os que não foram, foi encontrada diferença estatisticamente significativa apenas para o quociente de inteligência. Conclusão: O aleitamento materno está associado a níveis mais baixos de CCL11, escores mais altos de quociente de inteligência e a esquizofrenia. A quimiocina CCL11 é mais alta em quem não amamentou, especialmente nos esquizofrênicos.

O fenómeno da pós-globalização, que se tem feito sentir a uma larga escala na sociedade, traz consigo consequências sociais, políticas, económicas e culturais e exige um grande esforço de toda a comunidade para uma adaptação às novas exigências que acarreta. É neste contexto que surge o projeto CLIL4U, tendo como principal objetivo potenciar este diálogo de culturas desde uma idade precoce através de um processo de ensino-aprendizagem de língua e conteúdos integrados. Neste artigo, apresentaremos o projeto e seus pressupostos, consecução e avaliação. Utilizando uma metodologia de índole etnográfica e maioritariamente qualitativa, focaremos a nossa atenção nas representações que alunos, professores e encarregados de educação têm da implementação do projeto e sua monitorização.Os resultados mostram que os projetos CLIL (Content and Language Integrated Learning) podem ser uma mais-valia para instituições do 1º Ciclo do Ensino Básico, no que concerne a construção de um ensino centrado na aprendizagem pró-ativa de conteúdos.

The heated 5G network deployment race has already begun with the rapid progress in standardization efforts, backed by the current market availability of 5G-enabled network equipment, ongoing 5G spectrum auctions, early launching of non-standalone 5G network services in a few countries, among others. In this paper, we study current and future wireless networks from the viewpoint of energy efficiency (EE) and sustainability to meet the planned network and service evolution toward, along, and beyond 5G, as also inspired by the findings of the EU Celtic-Plus SooGREEN Project. We highlight the opportunities seized by the project efforts to enable and enrich this green nature of the network as compared to existing technologies. In specific, we present innovative means proposed in SooGREEN to monitor and evaluate EE in 5G networks and beyond. Further solutions are presented to reduce energy consumption and carbon footprint in the different network segments. The latter spans proposed virtualized/cloud architectures, efficient polar coding for fronthauling, mobile network powering via renewable energy and smart grid integration, passive cooling, smart sleeping modes in indoor systems, among others. Finally, we shed light on the open opportunities yet to be investigated and leveraged in future developments.

Wi-Fi networks are becoming more and more ubiquitous and represent a substantial source of energy consumption around the globe, mainly when it comes to Access Points (APs). There has been some work done on the characterization of the power consumption of Wi-Fi APs and network interface cards (NICs), and the power usage of these devices under different configurations and standards but mostly using legacy standards. A detailed AP power consumption analysis, exploring the whole set of degrees of freedom and capabilities of these devices is lacking in the state of the art. In this paper, we present a thorough power consumption analysis, covering the configuration options available in enterprise Wi-Fi APs from the three major vendors on the market. The goal is to understand how the power consumption of an AP varies with the different configurations, and provide insights on the parameters that significantly affect the AP power consumption. The obtained experimental results confirm previous state-of-the-art conclusions but contradict some of the studies and results found in the literature, while updating results and conclusions taken in the past to the most recent standards, configurations, and data rates available today. The analysis provided herein is a valuable source of information for deriving new AP power consumption models and designing energy-efficient Wi-Fi networks.

The main goal of this work is to propose a generic model of an urban vertical garden that can be folded and that can adapt to different types of spaces, taking advantage of the natural resources to the practice of urban agriculture. The methodology of this research obeys five main ideas, the relation between wasted space, man, living space and sustainable design in the production of biological products. The main findings are, improving the quality of life by promoting the practice of urban agriculture; the integrated management of sustainability components in a global market. This case study is pioneer in the Portuguese researches about foldable urban vertical gardens. The authors believe that it can be useful in the creation of a Portuguese guideline for the integration of controlled plant foods production. It promises environmental benefits resulting from the design and development of a product that includes new technologies and the choice of reusable materials.

O objetivo do estudo foi desenvolver e validar equação preditiva de massa livre de gordura por bioimpedanciometria para homens da população brasileira. Foram avaliados 203 homens de 20 a 59 anos de idade, divididos aleatoriamente em dois grupos: desenvolvimento (102) e validação cruzada (101). A técnica padrão foi a Abortometria radiológica de dupla energia (DEXA). Teste t pareado, regressão múltipla e plotagem de Bland-Altman foram utilizados para testar a validade do modelo proposto. A equação derivada pelo estudo foi: MLG = 17,57 + 0,348 (Estatura2/Resistência) + 0,513 Massa Corporal – 0,283 Perímetro Abdominal + 0,079 Reatância – 0,038 idade; coeficiente de correlação de concordância – CCC = 0,937; r2= 0,89; erro padrão de estimativa – EPE = 2,55 kg; erro puro – EP = 2,51 kg. Com isso, a nova equação proposta proporciona estimativa válida da massa livre de gordura, sendo recomendada para homens com características semelhantes.

A Ficção Interativa é uma forma de experiência digital que confere ao jogador/interator poder de agenciamento (agency) por meio das suas decisões, com consequências diretas sobre a configuração narrativa da obra. Esta forma de medialidade veio trazer novos desafios e subverter noções tradicionais de representação narrativa (moldadas sobretudo a partir do campo da ficção impressa), na medida em que a estrutura não-linear abre caminho a percursos diferentes de ação, dependendo do desempenho e das escolhas do interator. Neste contexto, compreende-se que a natureza confi gurável da personagem, quer como entidade ficcional com determinadas propriedades diegéticas, quer como peça fundamental da mecânica do jogo, constitua objeto de crescente atenção por parte da comunidade académica e dos agentes envolvidos no desenvolvimento de videojogos e de Ficção Interativa. O processo de construção da personagem, independentemente do seu estatuto específico (PC: personagem jogável; NPC: personagem não-jogável), assume aqui uma especificidade própria e levanta problemas que habitualmente não surgem com outros produtos literários e culturais, mesmo do contexto contemporâneo. Mais do que prolongar o aceso debate em torno do grau de adequação deste meio para narrar histórias que levou à formação de duas abordagens teóricas distintas – a ludológica e a narratológica –, importa desenvolver um método holístico capaz de congregar diversos ângulos de análise, reconhecendo que os videojogos tendem a desenvolver modelos narrativos complexos, mas que não podem subsistir sem uma estrutura consistente e um sistema de regras. De modo particular, os projetos recentes de Ficção Interativa com recurso a dispositivos de Inteligência Artificial que permitem selecionar, com base em procedimentos computacionais, eventos de modo coerente segundo o tipo de intervenção do interator e tirar o máximo partido da tensão narrativa levantam novas questões no que respeita à ontologia da personagem, ao seu potencial de manifestação de efeitos estéticos e à sua capacidade de gerar envolvimento emocional.

We consider the analytic continuation of Euclidean propagator data obtained from 4D simulations to Minkowski space. In order to perform this continuation, the common approach is to first extract the Källén-Lehmann spectral density of the field. Once this is known, it can be extended to Minkowski space to yield the Minkowski propagator. However, obtaining the Källén-Lehmann spectral density from propagator data is a well known ill-posed numerical problem. To regularise this problem we implement an appropriate version of Tikhonov regularisation supplemented with the Morozov discrepancy principle. We will then apply this to various toy model data to demonstrate the conditions of validity for this method, and finally to zero temperature gluon and ghost lattice QCD data. We carefully explain how to deal with the IR singularity of the massless ghost propagator. We also uncover the numerically different performance when using two—mathematically equivalent—versions of the Källén-Lehmann spectral integral.

It was with pleasure that I accepted the invitation to be guest editor of volume 9 of plaNext. This publishing initiative of AESOP Young Academics Network, almost reaching a dozen, has an already remarkable amount of knowledge produced and shared, fulfilling the aim based on the strategic vectors referred by Francesco Lo Piccolo in the editorial of the first volume of plaNext: to make Young Academics’ research products more visible; to bring together young academics from Europe and beyond, sharing new research perspectives, intersecting planning with other academic fields; and to combine open access environment with high quality materials (Lo Piccolo, 2015). This could not be truer when looking to contents of past and present volumes of plaNext, by giving visibility to more peripheral planning and research contexts, crossing perspectives from various disciplines while looking at European planning issues and not only.

VIII Congreso Iberoamericano de Nutrición. ¿Nutrición basada en la videncia o en la evidencia?

Se analizan las publicaciones sobre Ciencia Abierta de España y Portugal en la base de datos SCOPUS. A través de indicadores bibliométricos y altmétricos se estudia la repercusión de la producción en redes sociales. Entre 2000 y 2016 se detectaron 1273 documentos sobre el tema en ambos países, concentrados especialmente en el último quinquenio. Destacan las publicaciones sobre Open data y las temáticas de mayor producción han sido Computer Science y Social Science. Un tercio de las publicaciones con DOI ha tenido repercusión en las redes sociales siendo Twitter el medio que concentra mayor número de menciones. Si bien una tercera parte de los documentos se publicó en acceso abierto, no se detectó relación entre este indicador y la presencia en redes sociales.

Many studies have addressed the issue of migration and displacement, one of the main geopolitical challenges of our time, but far fewer have analysed it using an interdisciplinary framework and selecting literary and artistic practices from a transnational context. !e purpose of this paper is to describe the way in which the artists use the specificity of medium and mechanism in their projects to reflect on the current migratory crisis and forced migration, to critique the securitization or the politics of immigration in recent years and to explore a tactical use of tech-nologies in order to expand public consciousness and political debate. It is directly focused on the migratory processes involving Mexicans who go to the United States and its connection to the complex political imaginary of these regions, and on the relationship between identity and marginalization, particularly the influence of the conceptualization of migrants as the Other in contemporary European society. Based on the analysis of a series of case studies both from a theoretical and from an artistic point of view –, this research intends to understand how these projects explore forms of critical intervention, dissent or disruption of a dominant semiotic regime by stimulating critical thinking about consolidated narratives and sign systems of identity and difference.

Several reports have shown that stress during lactation causes long-term metabolic and hormonal disruptions. In this study, we designed experiments to evaluate the effects of stress during lactation on the abundance of Type 1 cannabinoid/endocannabinoid receptors (CB1R) in epididymal fat and liver and development of insulin resistance in adult mice. During the whole lactation, male mice pups were daily subcutaneously injected (days 1–21) with a saline solution to produce a soft nociceptive stress (NS). Mice body weight and food intake were periodically evaluated. Adult animals were subsequently subjected to an insulin tolerance test and some days later sacrificed to evaluate the amount of epididymal fat and abundance of CB1R and adipophilin in liver and epididymal adipose tissue. Lipoprotein lipase (LPL) activity and circulating levels of leptin, adiponectin, and corticosterone were also evaluated. In this model, NS during lactation significantly increased the amount of epididymal fat and induced insulin resistance in adult mice. In addition, a significantly increased abundance of CB1R and adipophilin in epididymal fat and liver was observed, together with elevated circulating levels of leptin and corticosterone. Adult NS animals also had low plasmatic adiponectin and, although nonsignificant, had a sustained trend to a greater LPL activity associated with epididymal fat. These results indicate that increased abundance of CB1R in liver and epididymal fat alters tissue functionality likely associated with development of systemic metabolic alterations such as insulin resistance in adult mice. All these pathophysiological facts are long-term consequences of nociceptive stress during lactation.

Skin biopsies were collected from three male patients (age 45, 44 and 44) with clinically diagnosed schizophrenia. The patients were diagnosed according to DSM-5 criteria by a trained psychiatrist. Dermal fibroblast cell lines were established and expanded for subsequent reprogramming procedures. Induced pluripotent stem (iPS) cells were derived using the integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, containing Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc.

O presente estudo versa sobre a questão, colocada em sede de História do Direito e na Sociologia, da origem etimológica da palavra “risco”, que acabaria por, entre outros significados, caracterizar as chamadas Sociedades do Risco. Nele tratamos da história e evolução (e aplicação) da palavra, aparentemente ligada aos seguros marítimos, do Tratado de Seguros de Pedro de Santarém e, fundamentalmente da história do LLoyd’s. A questão não possui resposta cabal a final, mas constitui, esperamos, um desafio para os eventuais interessados a investigarem sobre a matéria.

This study updates the geographic distributions of phlebotomine species in Central-West Brazil and analyses the climatic factors associated with their occurrence. The data were obtained from the entomology services of the state departments of health in Central-West Brazil, scientific collections and a literature review of articles from 1962-2014. Ecological niche models were produced for sandfly species with more than 20 occurrences using the Maxent algorithm and eight climate variables. In all, 2,803 phlebotomine records for 127 species were analysed. Nyssomyia whitmani,Evandromyia lenti and Lutzomyia longipalpiswere the species with the greatest number of records and were present in all the biomes in Central-West Brazil. The models, which were produced for 34 species, indicated that the Cerrado areas in the central and western regions of Central-West Brazil were climatically more suitable to sandflies. The variables with the greatest influence on the models were the temperature in the coldest months and the temperature seasonality. The results show that phlebotomine species in Central-West Brazil have different geographical distribution patterns and that climate conditions in essentially the entire region favour the occurrence of at least one Leishmania vector species, highlighting the need to maintain or intensify vector control and surveillance strategies.

Abstract Background The genus Evandromyia is widely found in Brazil, but occurs mainly in Brazilian savannah. To date 13 species have been described in the subgenus Aldamyia. Here we described a new species of Evandromyia (Aldamyia) collected in the State of Mato Grosso do Sul, Brazil. Methods Measurements were made using a micrometer eyepiece on an Olympus CH-2 binocular microscope and drawings were executed with the aid of a camera lucida. Results The new species, Evandromyia orcyi sp. nov., is closely related to Evandromyia lenti, Evandromyia carmelinoi and Evandromyia evandroi, however, characteristics of the male terminalia and female spermathecae distinguish it from other species of the genus Evandromyia. Conclusion With the description of Evandromyia orcyi sp. nov., six species of the subgenus Aldamyia have been reported from the State of Mato Grosso do Sul.

Objective: To report the results of a workshop regarding asthma management programs and centers (AMPCs) in Brazil, so that they can be used as a tool for the improvement and advancement of current and future AMPCs. Methods: The workshop consisted of five presentations and the corresponding group discussions. The working groups discussed the following themes: implementation of asthma management strategies; human resources needed for AMPCs; financial resources needed for AMPCs; and operational maintenance of AMPCs. Results: The workshop involved 39 participants, from all regions of the country, representing associations of asthma patients (n = 3), universities (n = 7), and AMPCs (n = 29). We found a direct relationship between a lack of planning and the failure of AMPCs. Based on the experiences reported during the workshop, the common assumptions about AMPCs in Brazil were the importance of raising awareness of managers; greater community participation; interdependence between primary care and specialized care; awareness of regionalization; and use of medications available in the public health system. Conclusions: Brazil already has a core of experience in the area of asthma management programs. The implementation of strategies for the management of chronic respiratory disease and their incorporation into health care system protocols would seem to be a natural progression. However, there is minimal experience in this area. Joint efforts by individuals with expertise in AMPCs could promote the implementation of asthma management strategies, thus speeding the creation of treatment networks, which might have a multiplier effect, precluding the need for isolated centers to start from zero.

This paper focuses on the role of infrastructures in place-making. We approach the concept of urban discontinuity, ways to produce and read landscapes and new paradigms in terms of urban form. The impact of infrastructures in landscape is based on Lisbon Metropolitan Area recent infrastructures. In order to discuss solutions for the impact of infrastructures in landscape, the relation between accessibilities, urban grid and urban tissue and the production of consolidated, non-consolidated and traced public spaces will be approached. The paper focuses on four nodes on the motorway A2, the main access to Lisbon by south. To conclude, a model to deal with urban discontinuities will be presented, integrating as a new element the concept of new urban structure.

A composição do desenho digital foi inspirada no progresso das aulas de Análise do Movimento Humano nas Atividades Cotidianas do curso de graduação em Terapia Ocupacional da Universidade Federal do Rio de Janeiro, UFRJ, lecionadas pela professora doutora Ana Paula Martins Cazeiro.A intenção foi expressar o processo de compreensão da sensibilidade nos estudos terapêuticos ocupacionais acerca da reabilitação física, na perspectiva de um aluno que não imaginava encontrar tamanho potencial de criação e produção de vida nas práticas até então ainda desconhecidas por este.

Todas as atividades culturais são um instrumento privilegiado na construção de representações que, pela geografia e pela história, sustentam a ideia de comunidade. O período filipino e a Restauração vieram exacerbar sentimentos de orgulho etnocêntrico e mostrar que a reivindicação de independência política tinha subjacente uma consciência identitária própria. Longe de ser uma especificidade das elites sociais e políticas, assume-se como dinâmica transversal que atravessa todo o espectro cultural que vai das tradições populares ao trabalho de raiz intelectual, demonstrando a existência de laços de solidariedade suficientemente fortes entre os membros desta comunidade que se via como portuguesa e, logo, diferente da castelhana. Este artigo procura abordar textos satíricos que exploram factos e circunstâncias dessa conjuntura histórica.

The aim was to report on a rare case of osteochondroma of the left ischium, which evolved with compression of the sciatic nerve, thus causing sciatic pain in the homolateral lower limb. The patient was female and presented sciatic pain that was treated clinically for one year. However, the pain evolved with increasing intensity and worsened with hip movement. This was associated with diminished motor force and paresthesia of the homolateral lower limb. Radiological investigation of the region showed a bone lesion in the external portion of the left ischium, in the path of the sciatic nerve. Tomographic reconstruction showed cortical continuity with the bone of origin, i.e., a pattern characteristic of osteochondroma. En-bloc resection of the lesion was performed using the Kocher-Langerbeck route, and the anatomopathological analysis proved that it was an osteochondroma. The patient's neurological symptoms improved and, after two months of follow-up, she remained asymptomatic and without any signs of recurrence. Since osteochondroma is the commonest benign bone tumor, it should be taken into consideration in the diagnostic investigation of compressive tumor lesions that could affect the sciatic nerve.

Descrever contribuições à técnica da cirurgia de artroplastia total em displasias do desenvolvimento do quadril grave, por meio da reconstrução acetabular com o uso de enxerto autólogo e encurtamento femoral feito com osteotomia subtrocantérica em V invertido. Paciente submetido a artroplastia total do quadril esquerdo em janeiro de 2003. Foi usada a classificação de Eftekhar e o paciente era do tipo D, luxação inveterada. Incorporação do enxerto no teto acetabular e osteotomia femoral. Acrescentamos a fixação do enxerto da cabeça femoral no acetábulo com placa do tipo Allis, que contribui para maior resistência do sistema, e a cerclagem com fio de aço no enxerto ósseo junto à osteotomia subtrocantérica, que diminui o risco de pseudoartrose. Essa técnica demonstrou eficácia e permitiu a resolução imediata do caso com melhoria da dor e da amplitude de movimento do quadril. Permitiu também a reconstrução do déficit ósseo acetabular, a recomposição do comprimento do membro (sem risco aumentado de lesão neurovascular) e a recuperação da biomecânica do quadril com a reparação do centro de rotação normal.

To understand the geographic distribution of visceral leishmaniasis (VL) in the state of Mato Grosso do Sul (MS), Brazil, both the climatic niches of Lutzomyia longipalpis and VL cases were analysed. Distributional data were obtained from 55 of the 79 counties of MS between 2003-2012. Ecological niche models (ENM) of Lu. longipalpis and VL cases were produced using the maximum entropy algorithm based on eight climatic variables. Lu. longipalpis showed a wide distribution in MS. The highest climatic suitability for Lu. longipalpis was observed in southern MS. Temperature seasonality and annual mean precipitation were the variables that most influenced these models. Two areas of high climatic suitability for the occurrence of VL cases were predicted: one near Aquidauana and another encompassing several municipalities in the southeast region of MS. As expected, a large overlap between the models for Lu. longipalpis and VL cases was detected. Northern and northwestern areas of MS were suitable for the occurrence of cases, but did not show high climatic suitability for Lu. longipalpis . ENM of vectors and human cases provided a greater understanding of the geographic distribution of VL in MS, which can be applied to the development of future surveillance strategies.

Introduction A sero-epidemiological survey was undertaken to detect the circulation of arboviruses in free-living non-human primates. Methods Blood samples were obtained from 16 non-human primates (13 Sapajus spp. and three Alouatta caraya) that were captured using terrestrial traps and anesthetic darts in woodland regions in the municipalities of Campo Grande, Aquidauana, Jardim, Miranda and Corumbá in the State of Mato Grosso do Sul, Brazil. The samples were sent to the Instituto Evandro Chagas (IEC) in Ananindeua, Pará, Brazil, to detect antibodies against 19 species of arboviruses using a hemagglutination inhibition test (HI). Results Of the 16 primates investigated in the present study, five (31.2%) were serologically positive for an arbovirus. Of these five, two (12.5%) exhibited antibodies to the Flavivirus genus, one (6.2%) exhibited a monotypic reaction to Cacipacoré virus, one (6.2%) was associated with Mayaro virus, and one (6.2%) was positive for Oropouche virus. Conclusions Based on the positive serology observed in the present study, it was possible to conclude that arboviruses circulate among free-living primates. The viruses in the areas studied might have been introduced by infected humans or by primates from endemic or enzootic areas. Studies of this nature, as well as efficient and continuous surveillance programs, are needed to monitor viral activities in endemic and enzootic regions.

Liriomyza spp. é considera praga em várias culturas no Brasil. As larvas confeccionam minas nas folhas, reduzem a área fotossintética, causam murcha e queda precoce de folhas. Objetivou-se neste trabalho reportar o primeiro ataque de Liriomyza spp., seu dano e a sobrevivência em cebola (Allium cepa L.) no Alto Paranaíba-MG. A sua ocorrência e o prejuízo foram detectados em cinco avaliações em 12 lavouras de cebola (50 plantas/ha). Já a sobrevivência foi determinada em condições de temperatura controlada. Observou-se a presença de Liriomyza spp. causando minas serpenteadas, com relação direta e negativa do número de minas com a produtividade, com prejuízo de 120 kg ha-1 e com taxa de sobrevivência de larva-pupa (57,89%) e pupa-adulto (63,63%). Este fitófago pode se tornar praga séria nesta cultura. First Occurrence of Leafminer Causing Damage in Onion at Alto Paranaíba Abstract. Liriomyza spp. is considered pests in various crops in Brazil. The larvae mine the leaves cook up, reduce the photosynthetic area, cause wilting and premature leaf drop. The objective of this study report the first attack of Liriomyza spp., its damage and survival in onion (Allium cepa L.) in Alto Parnaíba-MG. Their occurrence and injury ratings were detected in five of onion crops in 12 (50 plants/ha). Since survival was determined under conditions of controlled temperature. We observed the presence of Liriomyza spp. causing serpentine mines, with direct and negative relationship with the number of mines productivity, a loss of 120 kg ha-1 and survival rate of larva-pupa (57.89%) and pupa-adult (63.63%). Thus, this phytophagous insect can become a serious pest in this crop.

We estimated the geographic distributions of triatomine species in Central-West Region of Brazil (CW) and analysed the climatic factors influencing their occurrence. A total of 3,396 records of 27 triatomine species were analysed. Using the maximum entropy method, ecological niche models were produced for eight species occurring in at least 20 municipalities based on 13 climatic variables and elevation. Triatoma sordida and Rhodnius neglectus were the species with the broadest geographic distributions in CW Brazil. The Cerrado areas in the state of Goiás were found to be more suitable for the occurrence of synanthropic triatomines than the Amazon forest areas in the northern part of the state of Mato Grosso. The variable that best explains the evaluated models is temperature seasonality. The results indicate that almost the entire region presents climatic conditions that are appropriate for at least one triatomine species. Therefore, it is recommended that entomological surveillance be reinforced in CW Brazil.

Fauna of phlebotomine sand flies (Diptera, Psychodidae) in areas with endemic American cutaneous leishmaniasis in the State of Mato Grosso do Sul, Brazil. The aim of this study was to investigate the ecological aspects of the main vectors of American cutaneous leishmaniasis (ACL) in four monitoring stations situated in the municipalities of Naviraí, Nova Andradina, Novo Horizonte do Sul and Rio Verde de Mato Grosso. For each monitoring station, the captures of sand flies were undertaken each month from July 2008 to June 2010 using CDC and Shannon traps. The CDC traps were installed simultaneously for three consecutive nights in three collection sites: intradomicile, peridomicile and edge of the forest. A Shannon trap was installed from dusk to 10 pm, inside the forest, one night per month. A total of 7,651 sand flies belonging to nine genera and twenty-nine species were captured. Nyssomyia neivai (52.95%), Psathyromyia hermanlenti (10.91%), Psathyromyia runoides (9.16%), Nyssomyia whitmani (7.95%), Psathyromyia aragaoi (4. 89%), Nyssomyia antunesi (3.14%) and Evandromyia bourrouli (2.20%) were the most frequent species. Approximately 65% of the sand flies were collected in the forest environment. The municipalities presented significantly different indexes of species diversity. Naviraí presented the lowest species diversity index, however, it showed the highest abundance. Novo Horizonte do Sul had the highest species diversity index, but the lowest abundance (< 5%). It is noteworthy the occurrence of vector species of Leishmania in the areas studied, especially in Naviraí, where Ny. neivai presented high frequencies which may explain the increased number of ACL cases in this municipality.