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1. Synergism and antagonism between Bacillus thuringiensis Vip3A and Cry1 proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda.

Author

Ana Rita Nunes Lemes, Camila Chiaradia Davolos, Paula Cristina Brunini Crialesi Legori, Odair Aparecido Fernandes, Juan Ferré, Manoel Victor Franco Lemos, and Janete Apparecida Desiderio

Subjects
Medicine, Science
Abstract

Second generation Bt crops (insect resistant crops carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some of the new combinations involve co-expression of cry and vip genes. Because Cry and Vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two classes of toxins. Three members of the Cry1 class of proteins and three from the Vip3A class were tested against Heliothis virescens for possible interactions. At the level of LC50, Cry1Ac was the most active protein, whereas the rest of proteins tested were similarly active. However, at the level of LC90, Cry1Aa and Cry1Ca were the least active proteins, and Cry1Ac and Vip3A proteins were not significantly different. Under the experimental conditions used in this study, we found an antagonistic effect of Cry1Ca with the three Vip3A proteins. The interaction between Cry1Ca and Vip3Aa was also tested on two other species of Lepidoptera. Whereas antagonism was observed in Spodoptera frugiperda, synergism was found in Diatraea saccharalis. In all cases, the interaction between Vip3A and Cry1 proteins was more evident at the LC90 level than at the LC50 level. The fact that the same combination of proteins may result in a synergistic or an antagonistic interaction may be an indication that there are different types of interactions within the host, depending on the insect species tested.

Published
2014
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2. Interação de proteínas Cry1 e Vip3A de Bacillus thuringiensis para controle de lepidópteros-praga

Author

Ana Rita Nunes Lemes, Paula Cristina Brunini Crialesi-Legori, Odair Aparecido Fernandes, Suzana Cristina Marucci, Camila Chiaradia Davolos, Manoel Victor Franco Lemos, Janete Apparecida Desidério, and Universidade Estadual Paulista (Unesp)

Subjects
resistance management, biology, Chrysodeixis includens, piramidação de genes, manejo da resistência, biology.organism_classification, Molecular biology, lcsh:S1-972, Anticarsia gemmatalis, Bacterial protein, gene pyramiding, sinergismo, synergism, Animal Science and Zoology, soja transgênica, Natural enemies, lcsh:Agriculture (General), transgenic soybean, Soja transgênica, Agronomy and Crop Science
Abstract

Made available in DSpace on 2014-09-30T18:18:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-02-01Bitstream added on 2014-10-01T14:04:14Z : No. of bitstreams: 1 S0100-204X2014000200079.pdf: 748597 bytes, checksum: 6b6622d0023ecd80832b406b6f8b79aa (MD5) O objetivo deste trabalho foi avaliar a suscetibilidade das lagartas Anticarsia gemmatalis (Lepidoptera: Erebidae) e Chrysodeixis includens (Lepidoptera: Noctuidae) às proteínas Cry1 e Vip3A, bem como determinar se há a interação entre essas proteínas no controle das duas espécies. Bioensaios com as proteínas isoladas e em combinações foram realizados, e as concentrações letais CL50 e CL90 foram estimadas para cada condição. As proteínas Cry1Aa, Cry1Ac e Vip3Af foram as mais efetivas no controle de A. gemmatalis, enquanto Cry1Ac, Vip3Aa e Vip3Af foram mais efetivas no de C. includens. As proteínas Cry1Ac e Cry1Ca causaram maior inibição do desenvolvimento das larvas sobreviventes à CL50, em ambas as espécies. Combinações entre Vip3A e Cry1 apresentam efeito sinérgico no controle das espécies e a combinação Vip3Aa+Cry1Ea destaca-se no controle de A. gemmatalis e C. includens. Essas proteínas combinadas são promissoras na construção de plantas piramidadas, para o controle simultâneo das pragas. The objective of this work was to evaluate the susceptibility of Anticarsia gemmatalis (Lepidoptera: Erebidae) and Chrysodeixis includens (Lepidoptera: Noctuidae) caterpillars to Cry1 and Vip3A proteins, as well as to determine if there is any interaction between these proteins on the control of the two species. Bioassays with both isolated and combined proteins were carried out, and lethal concentrations LC50 and LC90 were estimated for each condition. Cry1Aa, Cry1Ac, and Vip3Af were the more effective proteins for the control of A. gemmatalis, while Cry1Ac, Vip3Aa, and Vip3Af were more effective for the control of C. includens. Cry1Ac and Cry1Ca proteins caused the highest inhibition to the development of larvae that survived the LC50 dose in both species. Different combinations of Vip3A and Cry1 have synergistic effect in the control of both species, and the combination Vip3Aa + Cry1Ea showed an outstanding control of A. gemmatalis and C. includens. These proteins are promising for building pyramided plants for the simultaneous control of the pests. Universidade Estadual Paulista Departamento de Biologia Aplicada à Agropecuária Unesp Departamento de Fitossanidade Universidade Estadual Paulista Departamento de Biologia Aplicada à Agropecuária Unesp Departamento de Fitossanidade

Published
2014

3. Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

Author

Manoel Victor Franco Lemos, Camila Chiaradia Davolos, Patricia Hernández-Martínez, Janete Apparecida Desidério, Paula Cristina Brunini Crialesi-Legori, Juan Ferré, and Baltasar Escriche

Subjects
media_common.quotation_subject, Population, Bacillus thuringiensis, Biology, Moths, Diatraea saccharalis, Competition (biology), Lepidoptera genitalia, Insecticide Resistance, Hemolysin Proteins, Crambidae, Bacterial Proteins, Botany, Animals, education, Pest Control, Biological, Ecology, Evolution, Behavior and Systematics, media_common, education.field_of_study, Bacillus thuringiensis Toxins, fungi, food and beverages, biology.organism_classification, Endotoxins, Cry1Ac, PEST analysis
Abstract

Sugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac, Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managing this pest.

Published
2014

4. Interação de proteínas Cry1 e Vip3A de Bacillus thuringiensis para controle de lepidópteros-praga

Author

Paula Cristina Brunini Crialesi-Legori, Camila Chiaradia Davolos, Ana Rita Nunes Lemes, Suzana Cristina Marucci, Manoel Victor Franco Lemos, Odair Aparecido Fernandes, and Janete Apparecida Desidério

Subjects
Anticarsia gemmatalis, Chrysodeixis includens, manejo da resistência, piramidação de genes, sinergismo, soja transgênica, Agriculture (General), S1-972
Abstract

O objetivo deste trabalho foi avaliar a suscetibilidade das lagartas Anticarsia gemmatalis (Lepidoptera: Erebidae) e Chrysodeixis includens (Lepidoptera: Noctuidae) às proteínas Cry1 e Vip3A, bem como determinar se há a interação entre essas proteínas no controle das duas espécies. Bioensaios com as proteínas isoladas e em combinações foram realizados, e as concentrações letais CL50 e CL90 foram estimadas para cada condição. As proteínas Cry1Aa, Cry1Ac e Vip3Af foram as mais efetivas no controle de A. gemmatalis, enquanto Cry1Ac, Vip3Aa e Vip3Af foram mais efetivas no de C. includens. As proteínas Cry1Ac e Cry1Ca causaram maior inibição do desenvolvimento das larvas sobreviventes à CL50, em ambas as espécies. Combinações entre Vip3A e Cry1 apresentam efeito sinérgico no controle das espécies e a combinação Vip3Aa+Cry1Ea destaca-se no controle de A. gemmatalis e C. includens. Essas proteínas combinadas são promissoras na construção de plantas piramidadas, para o controle simultâneo das pragas.

Published
2014
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