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5 results on '"Paul A. Cardenio"'

1. Prevalence and distribution of bla CTX-M, bla SHV, bla TEM genes in extended- spectrum β- lactamase- producing E. coli isolates from broiler farms in the Philippines

Author

Romeo S. Gundran, Paul A. Cardenio, Marvin A. Villanueva, Fredelon B. Sison, Carolyn C. Benigno, Kwanchai Kreausukon, Duangporn Pichpol, and Veerasak Punyapornwithaya

Subjects
Broiler, bla CTX-M, E. coli, ESBL, bla SHV, bla TEM, Veterinary medicine, SF600-1100
Abstract

Abstract Background Antimicrobial resistance is a worldwide problem causing serious health threats. Escherichia coli is one of the most important bacteria that causes resistance problem. These bacteria produce an enzyme called extended-spectrum β-lactamase (ESBL) that allows it to become resistant to a wide variety of penicillins and cephalosporins. Currently, no information or published studies on ESBL-producing E.coli in broilers are available in the Philippines. This cross-sectional study was conducted to determine the prevalence and distribution of extended-spectrum β-lactamase (ESBL)-encoding genes, bla CTX-M, bla SHV, and bla TEM , among E. coli isolates from broiler farms in Luzon, Philippines. Results Results showed a farm prevalence of 66. 67%. A total of 69 (44.23%) ESBL-producing E. coli were isolated from boot swabs and cloacal swab samples from broiler farms. All major bla CTX-M groups except bla CTX-M-25 group were identified in the isolates. The most prevalent group was bla CTX-M-1, 72.46% (CI: 60.38–82.54%), followed by bla CTX-M-2, bla CTX-M-9 group and bla CTX-M-8. The bla TEM and bla SHV genes were identified in 57.97 and 27.54% of isolates, respectively. The bla CTX-M and bla TEM were the most common gene combinations (33.33%). Coexistence of bla CTX-M types was observed in 50 (73.53%) isolates. Conclusion This study shows the high prevalence, diversity of patterns and coexistence of ESBL genes in the E. coli isolates from cloacal and boot swabs from broiler farms which pose risks of possible transmission to the environment, other animals and human.

Published
2019
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2. Ultrasonographic features of liver, gallbladder, spleen, kidneys, and urinary bladder of rescued long-tailed macaques, Macacafascicularis

Author

Paul A. Cardenio, Emilia A. Lastica-Ternura, and Jezie A. Acorda

Subjects
Pathology, medicine.medical_specialty, Urinary bladder, medicine.anatomical_structure, General Veterinary, business.industry, Medicine, Spleen, Liver/Gallbladder, business
Abstract

This study was conducted to determine the ultrasonographic features of the liver, gallbladder, spleen, kidneys, and urinary bladder of 24 apparently healthy male and female long-tailed macaques (LTM) and the correlations of organ measurements with body weight and crown–rump length. The animals were grouped into 12 males and 12 females. Examination was performed in sedated LTM using an ultrasound machine with 6.0 MHz microconvex scanner. Ultrasound appearance, dimensions, and echotexture of the liver, gallbladder, kidneys, spleen, and urinary bladder were evaluated. The results showed that there were no sex-related differences in echo mean values, thickness, and length of the selected abdominal organs. The liver was hypoechoic to isoechoic with the right renal cortex, while spleen was isoechoic to hyperechoic to left renal cortex. Spleen was hypoechoic to isoechoic to the liver. Gallbladder and urinary bladder had hypoechoic to hyperechoic thin wall with anechoic lumen. There was a statistically significant and moderate positive correlation between the volume of the left kidney and body weight. The right kidney volume was greater than the left kidney and the total renal volume had a statistically significant and moderate positive correlation with body weight. This study established the reference values for ultrasonographic features of selected abdominal organs of rescued LTM.

Published
2020
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3. Correlations of serum biochemistry profile with ultrasonic histogram of liver, gallbladder, and kidneys and morphometry of rescued long‐tailed macaques ( Macaca fascicularis )

Author

Jezie A. Acorda, Paul A. Cardenio, and Emilia A. Lastica-Ternura

Subjects
Male, Serum, 040301 veterinary sciences, Philippines, Physiology, Kidney, Macaque, 0403 veterinary science, chemistry.chemical_compound, biology.animal, medicine, Animals, 0501 psychology and cognitive sciences, 050102 behavioral science & comparative psychology, Liver/Gallbladder, Blood urea nitrogen, Ultrasonography, Creatinine, General Veterinary, biology, business.industry, Gallbladder, 05 social sciences, Ultrasound, 04 agricultural and veterinary sciences, Macaca fascicularis, medicine.anatomical_structure, Liver, chemistry, Serum biochemistry, Animals, Zoo, Female, Animal Science and Zoology, business, Blood Chemical Analysis
Abstract

Background Serum biochemistry and ultrasonography can be useful diagnostic tools in evaluating the general health condition of long-tailed macaques in rescue and rehabilitation centers. Methods This study was conducted to determine and correlate the serum biochemistry profile of 24 apparently healthy male and female rescued long-tailed macaques (LTM) with the body weight, crown-rump length, and ultrasonic histogram of liver parenchyma, gallbladder lumen, and renal cortices. Results There were no sex-related differences in serum biochemistry values of aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, blood urea nitrogen, total cholesterol, and total protein. Creatinine was positively correlated with body weights and crown-rump length. Multiple weak positive and negative correlations of organ-specific serum parameters and mean ultrasonic histogram of liver, gallbladder, and kidneys were observed. Conclusion This study established the correlations of serum biochemistry profile with ultrasonic histogram of liver, gallbladder, and kidneys and morphometry of rescued LTM.

Published
2020
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4. Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines

Author

Romeo S. Gundran, Kwanchai Kreausukon, Roderick T. Salvador, Veerasak Punyapornwithaya, Fredelon B. Sison, Duangporn Pichpol, Carolyn Benigno, and Paul A. Cardenio

Subjects
Microbiology (medical), Pharmacology, 0303 health sciences, medicine.diagnostic_test, 030306 microbiology, Immunology, biochemical phenomena, metabolism, and nutrition, Biology, bacterial infections and mycoses, medicine.disease_cause, Microbiology, 03 medical and health sciences, Antibiogram, medicine, bacteria, Pig farms, Escherichia coli, 030304 developmental biology
Abstract

This cross-sectional study was conducted to determine the prevalence, antibiogram, and resistance profile of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) isolates from healthy...

Published
2020
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5. Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing

Author

Romeo S, Gundran, Paul A, Cardenio, Roderick T, Salvador, Fredelon B, Sison, Carolyn C, Benigno, Kwanchai, Kreausukon, Duangporn, Pichpol, and Veerasak, Punyapornwithaya

Subjects
Swine Diseases, Cross-Sectional Studies, Genes, Bacterial, Swine, Drug Resistance, Multiple, Bacterial, Philippines, Escherichia coli, Prevalence, Animals, Microbial Sensitivity Tests, Animal Husbandry, beta-Lactamases, Anti-Bacterial Agents
Abstract

This cross-sectional study was conducted to determine the prevalence, antibiogram, and resistance profile of extended-spectrum β-lactamase-producing

Published
2019

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