Search

Your search keyword '"Paul, A.J."' showing total 1,092 results
Start Over Author "Paul, A.J."
1,092 results on '"Paul, A.J."'

1. Increased perivascular fibrosis and pro-fibrotic cellular transition in intramyocardial blood vessels in myocardial infarction patients

Author

Zhu Jiang, Giulia Sorrentino, Suat Simsek, Joris J.T.H. Roelofs, Hans W.M. Niessen, and Paul A.J. Krijnen

Subjects
Myocardial infarction, Perivascular fibrosis, Pro-fibrotic cellular transition, Cardiovascular disease, Diseases of the circulatory (Cardiovascular) system, RC666-701
Abstract

Background and objectives: Structural and functional changes in the intramyocardial microcirculation increase the risk of myocardial infarction (MI). This study investigated intramyocardial perivascular fibrosis and pro-fibrotic cellular transitions in deceased acute and subacute MI patients to explore their involvement in the pathogenesis of MI. Methods: Left ventricular tissue (LV) was obtained from the infarction area of autopsied patients with acute-phase MI (3–6 h; n = 24), subacute-phase MI (5–14 days; n = 12), and noninfarcted controls (n = 14). Perivascular fibrosis and fibroblast activation protein (FAP) expression were quantified using (immuno)histochemistry. Fibroblast-like transitioning of vascular smooth muscle cells (VSMC) and endothelial cells (EC) was quantified using immunofluorescent microscopy. Results: Perivascular fibrosis was elevated in acute-phase (77.69 %) and subacute-phase (72.19 %: border zone 95.18 %: infarct core) MI patients (p

Published
2024
Full Text
View/download PDF

2. Application of adipose tissue-derived stem cell therapy with a clinically relevant dose does not significantly affect atherosclerotic plaque characteristics in a streptozotocin-induced hyperglycaemia mouse model

Author

Amber Korn, Suat Simsek, Mitchell D. Fiet, Ingeborg S.E. Waas, Hans W.M. Niessen, and Paul A.J. Krijnen

Subjects
Adipose tissue-derived stem cells, ApoE−/− mouse, Atherosclerosis, Circulating monocytes, Diabetes mellitus, Immunohisochemistry, Diseases of the circulatory (Cardiovascular) system, RC666-701
Abstract

Aims: Diabetes mellitus (DM) induces increased inflammation of atherosclerotic plaques, resulting in elevated plaque instability. Mesenchymal stem cell (MSC) therapy was shown to decrease plaque size and increase stability in non-DM animal models. We now studied the effect of MSC therapy in a streptozotocin-induced hyperglycaemia mouse model using a clinically relevant dose of adipose tissue-derived MSCs (ASCs). Methods: Hyperglycaemia was induced in male C57BL/6 ApoE−/− mice (n=24) via intraperitoneal streptozotocin (STZ) injection (0.05 mg/g bodyweight) for 5 consecutive days. 16 weeks after the first STZ injection, the mice received either 100,000 ASCs (n=9) or vehicle (n=14) intravenously. The effects of ASC treatment on the size and stability of aortic root atherosclerotic plaques were determined 4 weeks post-treatment via (immuno)histochemical analyses. Furthermore, plasma monocyte subsets within 3 days pre- and 3 days post-treatment, and 4 weeks post-treatment, were studied. Results: ASC treatment did not significantly affect atherosclerotic plaque size or intra-plaque inflammation. Although ASC-treated mice had a higher percentage of intra-plaque fibrosis (42.5±3.3%) compared to vehicle-treated mice (37.6±6.8%, p=0.07), this did not reach significance. Additionally, although differences in the percentages of circulating pro- and anti-inflammatory monocytes were observed after ASC treatment compared to pre-treatment (p=0.005), their levels did not differ significantly at any time point compared to vehicle-treated mice. Conclusions: ASC treatment with a clinically relevant dose did not significantly affect atherosclerotic plaque size or intra-plaque inflammation in a hyperglycaemia mouse model. Despite a borderline significant improvement in intraplaque fibrotic content, the potential of ASC treatment on atherosclerotic plaque stability in a diabetic environment remains to be determined.

Published
2024
Full Text
View/download PDF

15. Cardiac inflammation and microvascular procoagulant changes are decreased in second wave compared to first wave deceased COVID-19 patients

Author

Wu, Linghe, Baylan, Umit, van der Leeden, Britt, Schurink, Bernadette, Roos, Eva, Schalkwijk, Casper G., Bugiani, Marianna, van der Valk, Paul, van Rossum, Albert C., Zeerleder, Sacha S., Heunks, Leo M.A., Boon, Reinier A., de Boer, Onno J., van der Wal, Allard C., Niessen, Hans W.M., and Krijnen, Paul A.J.

Published
2022
Full Text
View/download PDF

17. Intraluminal release of citrullinated histone 3 from various cellular origins coincides with microvascular thrombosis in burn wounds

Author

van der Leeden, Britt, primary, Korkmaz, H. Ibrahim, additional, Vlig, Marcel, additional, Waas, Ingeborg S.E., additional, Boekema, Bouke K.H.L., additional, Hassan, Chopie, additional, van Zuijlen, Paul P.M., additional, Niessen, Hans W.M., additional, Gibbs, Susan, additional, and Krijnen, Paul A.J., additional

Published
2024
Full Text
View/download PDF

40. Myocardial infarction triggers cardioprotective antigen-specific T helper cell responses

Author

Rieckmann, Max, Delgobo, Murilo, Gaal, Chiara, Buchner, Lotte, Steinau, Philipp, Reshef, Dan, Gil-Cruz, Cristina, Horst, Ellis N. ter, Kircher, Malte, Reiter, Theresa, Heinze, Katrin G., Niessen, Hans W.M., Krijnen, Paul A.J., van der Laan, Anja M., Piek, Jan J., Koch, Charlotte, Wester, Hans- Jurgen, Lapa, Constantin, Bauer, Wolfgang R., Ludewig, Burkhard, Friedman, Nir, Frantz, Stefan, Hofmann, Ulrich, and Ramos, Gustavo Campos

Subjects
Mediation, Heart attack, Autoimmunity, Myosin, Heart, Antigenic determinants, Gene expression, T cells, Muscle proteins, Death, Antigens, Positron emission tomography, Genes, Autoimmune diseases, Health care industry
Abstract

T cell autoreactivity is a hallmark of autoimmune diseases but can also benefit self-maintenance and foster tissue repair. Here, we investigated whether heart-specific T cells exert salutary or detrimental effects in the context of myocardial infarction (MI), the leading cause of death worldwide. After screening more than 150 class II-restricted epitopes, we found that myosin heavy chain [alpha] (MYHCA) was a dominant cardiac antigen triggering post-MI [CD4.sup.+] T cell activation in Balb/c mice. Transferred [MYHCA.sub.614-629]-specific [CD4.sup.+] T cells (TCR-M cells) selectively accumulated in the myocardium and mediastinal lymph nodes (med-LNs) of infarcted mice, acquired a Treg phenotype with a distinct prohealing gene expression profile, and mediated cardioprotection. Myocardial Tregs were also detected in autopsy samples from patients who had had a MI. Noninvasive PET/CT imaging using a CXCR4 radioligand revealed enlarged med-LNs with increased cellularity in patients with MI. Notably, the med-LN alterations observed in MI patients correlated with the infarct size and cardiac function. Taken together, the results obtained in our study provide evidence that MI context induces prohealing T cell autoimmunity in mice and confirm the existence of an analogous heart/med-LN/T cell axis in patients with MI., Introduction Cardiology and immunology are disciplines born apart, with different goals and tenets. Nonetheless, a fertile association between these areas of knowledge has recently flourished, and leukocytes have now been [...]

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results