116 results on '"Paudice, P"'
Search Results
2. Be bold, start cold! cold formalin fixation of colorectal cancer specimens granted superior DNA and RNA quality for downstream molecular analysis
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Nano, Ennio, Gambella, Alessandro, Paudice, Michele, Garuti, Anna, Pigozzi, Simona, Valle, Luca, Grillo, Federica, and Mastracci, Luca
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- 2024
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3. General Tail Bounds for Non-Smooth Stochastic Mirror Descent
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Eldowa, Khaled and Paudice, Andrea
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Computer Science - Machine Learning ,Statistics - Machine Learning - Abstract
In this paper, we provide novel tail bounds on the optimization error of Stochastic Mirror Descent for convex and Lipschitz objectives. Our analysis extends the existing tail bounds from the classical light-tailed Sub-Gaussian noise case to heavier-tailed noise regimes. We study the optimization error of the last iterate as well as the average of the iterates. We instantiate our results in two important cases: a class of noise with exponential tails and one with polynomial tails. A remarkable feature of our results is that they do not require an upper bound on the diameter of the domain. Finally, we support our theory with illustrative experiments that compare the behavior of the average of the iterates with that of the last iterate in heavy-tailed noise regimes.
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- 2023
4. An Improved Uniform Convergence Bound with Fat-Shattering Dimension
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Colomboni, Roberto, Esposito, Emmanuel, and Paudice, Andrea
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Computer Science - Machine Learning ,Statistics - Machine Learning - Abstract
The fat-shattering dimension characterizes the uniform convergence property of real-valued functions. The state-of-the-art upper bounds feature a multiplicative squared logarithmic factor on the sample complexity, leaving an open gap with the existing lower bound. We provide an improved uniform convergence bound that closes this gap.
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- 2023
5. Active Learning of Classifiers with Label and Seed Queries
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Bressan, Marco, Cesa-Bianchi, Nicolò, Lattanzi, Silvio, Paudice, Andrea, and Thiessen, Maximilian
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Computer Science - Machine Learning - Abstract
We study exact active learning of binary and multiclass classifiers with margin. Given an $n$-point set $X \subset \mathbb{R}^m$, we want to learn any unknown classifier on $X$ whose classes have finite strong convex hull margin, a new notion extending the SVM margin. In the standard active learning setting, where only label queries are allowed, learning a classifier with strong convex hull margin $\gamma$ requires in the worst case $\Omega\big(1+\frac{1}{\gamma}\big)^{(m-1)/2}$ queries. On the other hand, using the more powerful seed queries (a variant of equivalence queries), the target classifier could be learned in $O(m \log n)$ queries via Littlestone's Halving algorithm; however, Halving is computationally inefficient. In this work we show that, by carefully combining the two types of queries, a binary classifier can be learned in time $\operatorname{poly}(n+m)$ using only $O(m^2 \log n)$ label queries and $O\big(m \log \frac{m}{\gamma}\big)$ seed queries; the result extends to $k$-class classifiers at the price of a $k!k^2$ multiplicative overhead. Similar results hold when the input points have bounded bit complexity, or when only one class has strong convex hull margin against the rest. We complement the upper bounds by showing that in the worst case any algorithm needs $\Omega\big(k m \log \frac{1}{\gamma}\big)$ seed and label queries to learn a $k$-class classifier with strong convex hull margin $\gamma$.
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- 2022
6. Regret Analysis of Dyadic Search
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Bachoc, François, Cesari, Tommaso, Colomboni, Roberto, and Paudice, Andrea
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Computer Science - Machine Learning ,Mathematics - Optimization and Control - Abstract
We analyze the cumulative regret of the Dyadic Search algorithm of Bachoc et al. [2022]., Comment: arXiv admin note: substantial text overlap with arXiv:2208.06720
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- 2022
7. High Probability Bounds for Stochastic Subgradient Schemes with Heavy Tailed Noise
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Parletta, Daniela A., Paudice, Andrea, Pontil, Massimiliano, and Salzo, Saverio
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Mathematics - Optimization and Control ,Statistics - Machine Learning ,90C25, 62L20 - Abstract
In this work we study high probability bounds for stochastic subgradient methods under heavy tailed noise. In this setting the noise is only assumed to have finite variance as opposed to a sub-Gaussian distribution for which it is known that standard subgradient methods enjoys high probability bounds. We analyzed a clipped version of the projected stochastic subgradient method, where subgradient estimates are truncated whenever they have large norms. We show that this clipping strategy leads both to near optimal any-time and finite horizon bounds for many classical averaging schemes. Preliminary experiments are shown to support the validity of the method., Comment: 39 pages
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- 2022
8. A Near-Optimal Algorithm for Univariate Zeroth-Order Budget Convex Optimization
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Bachoc, François, Cesari, Tommaso, Colomboni, Roberto, and Paudice, Andrea
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Mathematics - Optimization and Control ,Computer Science - Machine Learning - Abstract
This paper studies a natural generalization of the problem of minimizing a univariate convex function $f$ by querying its values sequentially. At each time-step $t$, the optimizer can invest a budget $b_t$ in a query point $X_t$ of their choice to obtain a fuzzy evaluation of $f$ at $X_t$ whose accuracy depends on the amount of budget invested in $X_t$ across times. This setting is motivated by the minimization of objectives whose values can only be determined approximately through lengthy or expensive computations. We design an any-time parameter-free algorithm called Dyadic Search, for which we prove near-optimal optimization error guarantees. As a byproduct of our analysis, we show that the classical dependence on the global Lipschitz constant in the error bounds is an artifact of the granularity of the budget. Finally, we illustrate our theoretical findings with numerical simulations.
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- 2022
9. Impact of formalin fixation on mismatch repair protein evaluation by immunohistochemistry
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Grillo, Federica, Ali, Murad, Paudice, Michele, Pigozzi, Simona, Anselmi, Giorgia, Scabini, Stefano, Sciallero, Stefania, Piol, Nataniele, and Mastracci, Luca
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- 2023
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10. Evaluating mismatch repair deficiency in colorectal cancer biopsy specimens
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Grillo, F., Paudice, M., Gambella, A., Bozzano, S., Sciallero, S., Puccini, A., Lastraioli, S., Dono, M., Parente, P., Vanoli, A., Angerilli, V., Fassan, M., and Mastracci, L.
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- 2023
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11. On Margin-Based Cluster Recovery with Oracle Queries
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Bressan, Marco, Cesa-Bianchi, Nicolò, Lattanzi, Silvio, and Paudice, Andrea
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Computer Science - Machine Learning ,Statistics - Machine Learning - Abstract
We study an active cluster recovery problem where, given a set of $n$ points and an oracle answering queries like "are these two points in the same cluster?", the task is to recover exactly all clusters using as few queries as possible. We begin by introducing a simple but general notion of margin between clusters that captures, as special cases, the margins used in previous work, the classic SVM margin, and standard notions of stability for center-based clusterings. Then, under our margin assumptions we design algorithms that, in a variety of settings, recover all clusters exactly using only $O(\log n)$ queries. For the Euclidean case, $\mathbb{R}^m$, we give an algorithm that recovers arbitrary convex clusters, in polynomial time, and with a number of queries that is lower than the best existing algorithm by $\Theta(m^m)$ factors. For general pseudometric spaces, where clusters might not be convex or might not have any notion of shape, we give an algorithm that achieves the $O(\log n)$ query bound, and is provably near-optimal as a function of the packing number of the space. Finally, for clusterings realized by binary concept classes, we give a combinatorial characterization of recoverability with $O(\log n)$ queries, and we show that, for many concept classes in Euclidean spaces, this characterization is equivalent to our margin condition. Our results show a deep connection between cluster margins and active cluster recoverability.
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- 2021
12. Multitask Online Mirror Descent
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Cesa-Bianchi, Nicolò, Laforgue, Pierre, Paudice, Andrea, and Pontil, Massimiliano
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Computer Science - Machine Learning - Abstract
We introduce and analyze MT-OMD, a multitask generalization of Online Mirror Descent (OMD) which operates by sharing updates between tasks. We prove that the regret of MT-OMD is of order $\sqrt{1 + \sigma^2(N-1)}\sqrt{T}$, where $\sigma^2$ is the task variance according to the geometry induced by the regularizer, $N$ is the number of tasks, and $T$ is the time horizon. Whenever tasks are similar, that is $\sigma^2 \le 1$, our method improves upon the $\sqrt{NT}$ bound obtained by running independent OMDs on each task. We further provide a matching lower bound, and show that our multitask extensions of Online Gradient Descent and Exponentiated Gradient, two major instances of OMD, enjoy closed-form updates, making them easy to use in practice. Finally, we present experiments which support our theoretical findings.
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- 2021
13. Exact Recovery of Clusters in Finite Metric Spaces Using Oracle Queries
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Bressan, Marco, Cesa-Bianchi, Nicolò, Lattanzi, Silvio, and Paudice, Andrea
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Computer Science - Machine Learning ,Statistics - Machine Learning - Abstract
We investigate the problem of exact cluster recovery using oracle queries. Previous results show that clusters in Euclidean spaces that are convex and separated with a margin can be reconstructed exactly using only $O(\log n)$ same-cluster queries, where $n$ is the number of input points. In this work, we study this problem in the more challenging non-convex setting. We introduce a structural characterization of clusters, called $(\beta,\gamma)$-convexity, that can be applied to any finite set of points equipped with a metric (or even a semimetric, as the triangle inequality is not needed). Using $(\beta,\gamma)$-convexity, we can translate natural density properties of clusters (which include, for instance, clusters that are strongly non-convex in $\mathbb{R}^d$) into a graph-theoretic notion of convexity. By exploiting this convexity notion, we design a deterministic algorithm that recovers $(\beta,\gamma)$-convex clusters using $O(k^2 \log n + k^2 (6/\beta\gamma)^{dens(X)})$ same-cluster queries, where $k$ is the number of clusters and $dens(X)$ is the density dimension of the semimetric. We show that an exponential dependence on the density dimension is necessary, and we also show that, if we are allowed to make $O(k^2 + k\log n)$ additional queries to a "cluster separation" oracle, then we can recover clusters that have different and arbitrary scales, even when the scale of each cluster is unknown., Comment: Accepted for presentation at the Conference on Learning Theory (COLT) 2021
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- 2021
14. Colorectal adenosquamous carcinoma: genomic profiling of a rare histotype of colorectal cancer
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Angerilli, Valentina, Parente, Paola, Businello, Gianluca, Vanoli, Alessandro, Paudice, Michele, Perrone, Giovanni, Munari, Giada, Govoni, Ilaria, Neri, Giuseppe, Rebellato, Elena, Parrella, Paola, Grillo, Federica, Mastracci, Luca, and Fassan, Matteo
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- 2023
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15. Robust Unsupervised Learning via L-Statistic Minimization
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Maurer, Andreas, Parletta, Daniela A., Paudice, Andrea, and Pontil, Massimiliano
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Computer Science - Machine Learning - Abstract
Designing learning algorithms that are resistant to perturbations of the underlying data distribution is a problem of wide practical and theoretical importance. We present a general approach to this problem focusing on unsupervised learning. The key assumption is that the perturbing distribution is characterized by larger losses relative to a given class of admissible models. This is exploited by a general descent algorithm which minimizes an $L$-statistic criterion over the model class, weighting small losses more. Our analysis characterizes the robustness of the method in terms of bounds on the reconstruction error relative to the underlying unperturbed distribution. As a byproduct, we prove uniform convergence bounds with respect to the proposed criterion for several popular models in unsupervised learning, a result which may be of independent interest.Numerical experiments with kmeans clustering and principal subspace analysis demonstrate the effectiveness of our approach., Comment: We have just uploaded a new version of the paper with a more relavant title " Robust Unsupervised Learning via L-statistic Minimization"
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- 2020
16. Exact Recovery of Mangled Clusters with Same-Cluster Queries
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Bressan, Marco, Cesa-Bianchi, Nicolò, Lattanzi, Silvio, and Paudice, Andrea
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Computer Science - Machine Learning ,Statistics - Machine Learning - Abstract
We study the cluster recovery problem in the semi-supervised active clustering framework. Given a finite set of input points, and an oracle revealing whether any two points lie in the same cluster, our goal is to recover all clusters exactly using as few queries as possible. To this end, we relax the spherical $k$-means cluster assumption of Ashtiani et al.\ to allow for arbitrary ellipsoidal clusters with margin. This removes the assumption that the clustering is center-based (i.e., defined through an optimization problem), and includes all those cases where spherical clusters are individually transformed by any combination of rotations, axis scalings, and point deletions. We show that, even in this much more general setting, it is still possible to recover the latent clustering exactly using a number of queries that scales only logarithmically with the number of input points. More precisely, we design an algorithm that, given $n$ points to be partitioned into $k$ clusters, uses $O(k^3 \ln k \ln n)$ oracle queries and $\tilde{O}(kn + k^3)$ time to recover the clustering with zero misclassification error. The $O(\cdot)$ notation hides an exponential dependence on the dimensionality of the clusters, which we show to be necessary thus characterizing the query complexity of the problem. Our algorithm is simple, easy to implement, and can also learn the clusters using low-stretch separators, a class of ellipsoids with additional theoretical guarantees. Experiments on large synthetic datasets confirm that we can reconstruct clusterings exactly and efficiently., Comment: To appear at NeurIPS 2020 (oral)
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- 2020
17. Counting mitoses in gastrointestinal stromal tumours (GISTs): variable practices in the real-world setting and their clinical implications
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Campora, Michela, Paudice, Michele, Gambella, Alessandro, Comandini, Danila, Parente, Paola, Sbaraglia, Marta, Dei Tos, Angelo Paolo, Grillo, Federica, and Mastracci, Luca
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- 2023
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18. Frozen Section of Placental Membranes and Umbilical Cord: A Valid Diagnostic Tool for Early-Onset Neonatal Sepsis Management
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Veronica Parrella, Michele Paudice, Michela Pittaluga, Alessandra Allodi, Ezio Fulcheri, Francesca Buffelli, Fabio Barra, Simone Ferrero, Cesare Arioni, and Valerio Gaetano Vellone
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neonatal sepsis ,placenta ,funisitis ,chorioamnionitis ,frozen section examination ,Medicine (General) ,R5-920 - Abstract
Early-onset neonatal sepsis (EONS), a serious infection in newborns within 3 days, is challenging to diagnose. The current methods often lack accuracy, leading to unnecessary antibiotics or delayed treatment. This study investigates the role of the frozen section examination of placental membranes and umbilical cord (FSMU) to improve EONS diagnosis in the daily lab practice. This retrospective study reviewed data from 59 neonates with EONS risk factors who underwent FSMU according to our institutional protocol. Concordance between the FSMU and the Final Pathological Report (FPR) was assessed. The FSMU demonstrated a high concordance (Kappa = 0.88) for funisitis diagnosis, with excellent accuracy (98.3%). A moderate concordance was observed for chorioamnionitis stage and grade. The FSMU shows promise as a rapid and accurate tool for diagnosing EONS, particularly for funisitis. This study suggests that the FSMU could be a valuable tool for EONS diagnosis, enabling a more judicious antibiotic use and potentially improving outcomes for newborns.
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- 2024
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19. Correlation Clustering with Adaptive Similarity Queries
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Bressan, Marco, Cesa-Bianchi, Nicolò, Paudice, Andrea, and Vitale, Fabio
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Computer Science - Machine Learning ,Statistics - Machine Learning - Abstract
In correlation clustering, we are given $n$ objects together with a binary similarity score between each pair of them. The goal is to partition the objects into clusters so to minimise the disagreements with the scores. In this work we investigate correlation clustering as an active learning problem: each similarity score can be learned by making a query, and the goal is to minimise both the disagreements and the total number of queries. On the one hand, we describe simple active learning algorithms, which provably achieve an almost optimal trade-off while giving cluster recovery guarantees, and we test them on different datasets. On the other hand, we prove information-theoretical bounds on the number of queries necessary to guarantee a prescribed disagreement bound. These results give a rich characterization of the trade-off between queries and clustering error.
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- 2019
20. HLA-G as a prognostic marker in stage II/III colorectal cancer: not quite there yet
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Gambella, Alessandro, Scabini, Stefano, Zoppoli, Gabriele, De Silvestri, Annalisa, Pigozzi, Simona, Paudice, Michele, Campora, Michela, Fiocca, Roberto, Grillo, Federica, and Mastracci, Luca
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- 2022
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21. Quantification of Women Who Could Benefit from Hormone Therapy after Endometrial Cancer Treatment: An Analysis of SEER Data
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Ambrogio P. Londero, Anjeza Xholli, Serena Bertozzi, Maria Orsaria, Michele Paudice, Laura Mariuzzi, and Angelo Cagnacci
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endometrial cancer ,endometrioid ,survival ,hormonal therapy ,premenopause ,early postmenopause ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Our primary aim was to estimate the magnitude of stage I endometrial cancer (EC) survivors that could benefit from hormonal therapy (HT). Our secondary aims were to assess EC incidence in women below 50 and below 60 over the years, and analyze the overall survival and any influencing factors. We analyzed the endometrioid EC data from the Surveillance, Epidemiology, and End Results (SEER) program according to women’s age, tumor stage, and grade. We analyzed the proportions of EC survivors below 50 and below 60 years of age and stratified those age groups by race. For age distribution and survival analysis SEER, 18 registries’ research data (2000–2018) were analyzed. We analyzed the SEER 12 registries’ research data (1992–2019) for incidence time trends. Our investigation found a 14% and 40% cumulative prevalence of stage I EC that occurs in women below 50 or 60 years, respectively. EC’s prevalence has progressively risen in recent decades, but cancer-specific mortality remains low. The increasing number of women affected by EC in premenopause or early postmenopause face an 18 years-survival rate of 96.86% and 95.73%, respectively. A significant proportion of low-grade EC survivors can potentially benefit from HT treatment, and this requires awareness of other aspects of their health or quality of life, in addition to cancer treatments.
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- 2022
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22. The fading guardian: clinical relevance of TP53 null mutation in high-grade serous ovarian cancers
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Chiara M. Biatta, Michele Paudice, Marco Greppi, Veronica Parrella, Alessia Parodi, Giuseppa De Luca, Gianna Maria Cerruti, Serafina Mammoliti, Cinzia Caroti, Paola Menichini, Gilberto Fronza, Silvia Pesce, Emanuela Marcenaro, and Valerio G. Vellone
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high grade serous ovarian carcinoma ,TP53 ,immunohistochemistry ,sanger sequencing ,ovarian cancer ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Backgroundwe evaluated the concordance between immunohistochemical p53 staining and TP53 mutations in a series of HGSOC. Moreover, we searched for prognostic differences between p53 overexpression and null expression groups.Methodspatients affected by HGSOC were included. For each case p53 immunohistochemical staining and molecular assay (Sanger sequencing) were performed. Kaplan-Meier survival analyses were undertaken to determine whether the type of TP53 mutation, or p53 staining pattern influenced overall survival (OS) and progression free survival (PFS).Results34 HGSOC were considered. All cases with a null immunohistochemical p53 expression (n=16) showed TP53 mutations (n=9 nonsense, n=4 in-frame deletion, n=2 splice, n=1 in-frame insertion). 16 out of 18 cases with p53 overexpression showed TP53 missense mutation. Follow up data were available for 33 out of 34 cases (median follow up time 15 month). We observed a significant reduction of OS in p53 null group [HR = 3.64, 95% CI 1.01-13.16].Conclusionimmunohistochemical assay is a reliable surrogate for TP53 mutations in most cases. Despite the small cohort and the limited median follow up, we can infer that HGSOC harboring p53 null mutations are a more aggressive subgroup.
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- 2023
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23. Label Sanitization against Label Flipping Poisoning Attacks
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Paudice, Andrea, Muñoz-González, Luis, and Lupu, Emil C.
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Statistics - Machine Learning ,Computer Science - Cryptography and Security ,Computer Science - Machine Learning - Abstract
Many machine learning systems rely on data collected in the wild from untrusted sources, exposing the learning algorithms to data poisoning. Attackers can inject malicious data in the training dataset to subvert the learning process, compromising the performance of the algorithm producing errors in a targeted or an indiscriminate way. Label flipping attacks are a special case of data poisoning, where the attacker can control the labels assigned to a fraction of the training points. Even if the capabilities of the attacker are constrained, these attacks have been shown to be effective to significantly degrade the performance of the system. In this paper we propose an efficient algorithm to perform optimal label flipping poisoning attacks and a mechanism to detect and relabel suspicious data points, mitigating the effect of such poisoning attacks.
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- 2018
24. Detection of Adversarial Training Examples in Poisoning Attacks through Anomaly Detection
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Paudice, Andrea, Muñoz-González, Luis, Gyorgy, Andras, and Lupu, Emil C.
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Statistics - Machine Learning ,Computer Science - Cryptography and Security ,Computer Science - Learning - Abstract
Machine learning has become an important component for many systems and applications including computer vision, spam filtering, malware and network intrusion detection, among others. Despite the capabilities of machine learning algorithms to extract valuable information from data and produce accurate predictions, it has been shown that these algorithms are vulnerable to attacks. Data poisoning is one of the most relevant security threats against machine learning systems, where attackers can subvert the learning process by injecting malicious samples in the training data. Recent work in adversarial machine learning has shown that the so-called optimal attack strategies can successfully poison linear classifiers, degrading the performance of the system dramatically after compromising a small fraction of the training dataset. In this paper we propose a defence mechanism to mitigate the effect of these optimal poisoning attacks based on outlier detection. We show empirically that the adversarial examples generated by these attack strategies are quite different from genuine points, as no detectability constrains are considered to craft the attack. Hence, they can be detected with an appropriate pre-filtering of the training dataset., Comment: 10 pages, 3 figures
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- 2018
25. Towards Poisoning of Deep Learning Algorithms with Back-gradient Optimization
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Muñoz-González, Luis, Biggio, Battista, Demontis, Ambra, Paudice, Andrea, Wongrassamee, Vasin, Lupu, Emil C., and Roli, Fabio
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Computer Science - Learning - Abstract
A number of online services nowadays rely upon machine learning to extract valuable information from data collected in the wild. This exposes learning algorithms to the threat of data poisoning, i.e., a coordinate attack in which a fraction of the training data is controlled by the attacker and manipulated to subvert the learning process. To date, these attacks have been devised only against a limited class of binary learning algorithms, due to the inherent complexity of the gradient-based procedure used to optimize the poisoning points (a.k.a. adversarial training examples). In this work, we rst extend the de nition of poisoning attacks to multiclass problems. We then propose a novel poisoning algorithm based on the idea of back-gradient optimization, i.e., to compute the gradient of interest through automatic di erentiation, while also reversing the learning procedure to drastically reduce the attack complexity. Compared to current poisoning strategies, our approach is able to target a wider class of learning algorithms, trained with gradient- based procedures, including neural networks and deep learning architectures. We empirically evaluate its e ectiveness on several application examples, including spam ltering, malware detection, and handwritten digit recognition. We nally show that, similarly to adversarial test examples, adversarial training examples can also be transferred across di erent learning algorithms.
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- 2017
26. Efficient Attack Graph Analysis through Approximate Inference
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Muñoz-González, Luis, Sgandurra, Daniele, Paudice, Andrea, and Lupu, Emil C.
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Computer Science - Cryptography and Security ,Computer Science - Artificial Intelligence ,Statistics - Machine Learning - Abstract
Attack graphs provide compact representations of the attack paths that an attacker can follow to compromise network resources by analysing network vulnerabilities and topology. These representations are a powerful tool for security risk assessment. Bayesian inference on attack graphs enables the estimation of the risk of compromise to the system's components given their vulnerabilities and interconnections, and accounts for multi-step attacks spreading through the system. Whilst static analysis considers the risk posture at rest, dynamic analysis also accounts for evidence of compromise, e.g. from SIEM software or forensic investigation. However, in this context, exact Bayesian inference techniques do not scale well. In this paper we show how Loopy Belief Propagation - an approximate inference technique - can be applied to attack graphs, and that it scales linearly in the number of nodes for both static and dynamic analysis, making such analyses viable for larger networks. We experiment with different topologies and network clustering on synthetic Bayesian attack graphs with thousands of nodes to show that the algorithm's accuracy is acceptable and converge to a stable solution. We compare sequential and parallel versions of Loopy Belief Propagation with exact inference techniques for both static and dynamic analysis, showing the advantages of approximate inference techniques to scale to larger attack graphs., Comment: 30 pages, 14 figures
- Published
- 2016
27. Methods for restoration of ki67 antigenicity in aged paraffin tissue blocks
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Grillo, Federica, Campora, Michela, Pigozzi, Simona, Bonadio, Silvia, Valle, Luca, Ferro, Jacopo, Paudice, Michele, Dose, Beatrice, and Mastracci, Luca
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- 2021
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28. New-Onset Cancer in the HF Population: Epidemiology, Pathophysiology, and Clinical Management
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Cuomo, Alessandra, Paudice, Francesca, D’Angelo, Giovanni, Perrotta, Giovanni, Carannante, Antonio, Attanasio, Umberto, Iengo, Martina, Fiore, Francesco, Tocchetti, Carlo Gabriele, Mercurio, Valentina, and Pirozzi, Flora
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- 2021
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29. Histopathological and Immunohistochemical Prognostic Factors in High-Grade Non-Endometrioid Carcinomas of the Endometrium (HG-NECs): Is It Possible to Identify Subgroups at Increased Risk?
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Michele Paudice, Chiara Maria Biatta, Giulia Scaglione, Alessia Parodi, Serafina Mammoliti, Melita Moioli, Maria Grazia Centurioni, Fabio Barra, Simone Ferrero, Franco De Cian, Katia Mazzocco, and Valerio Gaetano Vellone
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endometrial carcinoma ,immunohistochemistry ,prognosis ,Medicine (General) ,R5-920 - Abstract
Endometrial cancer is an emerging disease with an increase in prevalence of aggressive histotypes in recent years. Background: In the present study, potential histopathological and immunohistochemical prognostic markers were investigated. Consecutive cases of high-grade non-endometrioid carcinoma (HG-NEC) of the endometrium were considered. Methods: Each surgical specimen was routinely processed; the most significant block was selected for immunohistochemistry and tested for ER, PR, ki67, p53, E-cadherin, β-catenin, Bcl-2 and cyclin D1. For each immunomarker, the percentage of positive tumor cells was evaluated (%) and dichotomized as low and high according to the distribution in the study population. Follow-up was collected for disease-free survival (DFS) and overall survival (OS). Thirty-three cases were eligible: 19 resulted in FIGO I–II; 14 resulted in FIGO III–IV. Twelve patients suffered a recurrent disease (mean follow-up 24.6 months); 8 patients died of the disease (mean follow-up 26.6 months). Results: Women with recurrent disease demonstrated a significantly higher Bcl2% (35.84 ± 30.96% vs. 8.09 ± 11.56%; p = 0.0032) while DOD patients had higher ki67% (75 ± 13.09% vs. 58.6 ± 19.97%; p = 0.033) and Bcl2% of border significance (34.37 ± 34.99% vs. 13 ± 17.97%; p = 0.078). As expected, FIGO III–IV had a worse DFS (HR = 3.34; 95% CI: 1.1–10.99; p = 0.034) and OS (HR = 5.19; 95% CI: 1.27–21.14; p = 0.0217). Bcl-2-high patients (Bcl2 > 10%) demonstrated a significantly worse DFS (HR = 9.11; 95% CI: 2.6–32.4; p = 0.0006) and OS (HR = 7.63; 95% CI: 1.7–34; p = 0.0084); moreover, PR low patients (PR ≤ 10%) had significantly worse DFS (HR = 3.74; 95% CI: 1.2–11.9; p = 0.02). Conclusions: HG-NEC represents a heterogeneous group of endometrial aggressive neoplasms with a worrisome prognosis, often at an advanced stage at presentation. Bcl-2 and PR may represent promising markers to identify a subgroup of patients having an even worse prognosis requiring a careful and close follow-up.
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- 2023
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30. Efficacy and safety of prolonged-release hyaluronic acid derivative vaginal application in the postpartum period: a prospective randomised clinical trial
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Claudio Gustavino, Paolo Sala, Nadia Cusini, Brunella Gravina, Cecilia Ronzini, Diletta Marcolin, Valerio Gaetano Vellone, Michele Paudice, Rossella Nappi, Sergio Costantini, Simone Ferrero, and Fabio Barra
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Prolonged-release hyaluronic acid derivative vaginal gel ,hydeal-D ,sexual function ,postnatal depression ,vaginal maturation index ,vaginal dryness ,Medicine - Abstract
AbstractIntroduction In puerperium, the hypoestrogenic state induced by delivery and subsequently sustained by lactation may lead to vaginal dryness, burning, and itching sensation, contributing to the onset of sexual dysfunction.Material and methods This was a prospective, randomized, controlled, open-label study (NCT04560283) for evaluating the effects of application of a prolonged-release hyaluronic acid derivative vaginal gel in restoring sexual function during the postpartum period. Eighty-five patients were randomized to apply prolonged-release Hydeal-D 0.2% vaginal gel (Fidia Farmaceutici, Abano Terme, Italy; n = 43) every three days for 12 consecutive weeks or expectant management (n = 42).Results Women undergoing treatment had a more elevate increase in Female Sexual Function Index (FSFI) total score (+15.1 ± 11.9 vs +6.5 ± 8.9, p < 0.001) and a higher decrease in vaginal pH (−1.2 ± 0.7 vs −0.2 ± 1.1; p < 0.001). Moreover, the proportion of vaginal smears with maturation index (VMI) >65 was significantly higher in patients treated (80.6% vs 35.3%; p = 0.004). Edinburgh Postnatal Depression Scale (EPDS) decreased significantly in both groups with no inter-group difference (p = 0.459). Only two cases (4.8%) of moderate vaginal burning sensation were reported in patients undergoing local vaginal therapy.Conclusions The results of our study demonstrated that hyaluronic acid derivative vaginal gel (Hydeal-D) was able to improve sexual function of puerperal women in the short-term treatment.KEY MESSAGEIn the puerperium, the hypoestrogenic state induced by delivery and subsequently sustained by lactation may lead to vaginal dryness, burning, and itching sensation, contributing to the onset of sexual dysfunction.Hydeal-D is a prolonged-release hyaluronic acid derivative characterised by elevated resistance to enzymatic breakdown. During puerperium, its local application may improve the vaginal microenvironment by ensuring a better migration and proliferation of cells involved in local tissue repair.Among puerperal women, Hydeal-D vaginal gel causes a significant improvement of sexual function, including desire, arousal, and lubrification, compared to expectant management. Furthermore, it leads to a decrease in vaginal pH and an increase of the trophic status of vaginal epithelium.
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- 2021
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31. Multiple rubbery nodules on the scalp
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Astrid Herzum, MD, Roberto Russo, MD, Emanuele Cozzani, MD, PhD, Michele Paudice, MD, Antonio Guadagno, MD, and Aurora Parodi, MD
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adenocarcinoma ,Brooke-Spiegler ,CYLD ,cylindroma ,spiradenoma ,trichoepithelioma ,Dermatology ,RL1-803 - Published
- 2021
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32. Pancytopenia in a Case of Aplastic Anaemia/Paroxysmal Nocturnal Haemoglobinuria Unmasked by SARS-CoV-2 Infection: A Case Report
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Arcangelo Iannuzzi, Antonio Parrella, Francesca De Ritis, Anna Cammarota, Lucia Berloco, Francesca Paudice, Giovanni D’Angelo, Emilio Aliberti, and Gabriella Iannuzzo
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COVID-19 ,Paroxysmal Nocturnal Haemoglobinuria ,PIGA gene ,aplastic anaemia ,Medicine (General) ,R5-920 - Abstract
During an acute SARS-CoV-2 infection, a diagnosis of Aplastic Anaemia associated with Paroxysmal Nocturnal Haemoglobinuria (AA/PNH) was made in a 78-year-old woman who had presented to the emergency department with severe pancytopenia. It is possible that she had subclinical AA/PNH that was unmasked during the acute COVID-19 infection, but we can also suspect a direct role of the virus in the pathogenesis of the disease, or we can hypothesize that COVID-19 infection changed the phosphatidylinositol glycan class A (PIGA) gene pathway.
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- 2022
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33. A Case of Four Synchronous Cutaneous Melanomas: Melanocortin 1 Receptor Polymorphisms and Excessive Sun Exposure
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Giulia Gasparini, Giulia Ciccarese, Emanuele Cozzani, William Bruno, Francesco Cabiddu, Margherita Cioni, Michele Paudice, and Aurora Parodi
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Dermatology ,RL1-803 - Abstract
Abstract is missing (Short communication)
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- 2020
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34. Report of Positive Placental Swabs for SARS-CoV-2 in an Asymptomatic Pregnant Woman with COVID-19
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Antonella Ferraiolo, Fabio Barra, Chiara Kratochwila, Michele Paudice, Valerio Gaetano Vellone, Elisabetta Godano, Serena Varesano, Giovanni Noberasco, Simone Ferrero, and Cesare Arioni
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COVID-19 ,SARS-CoV-2 ,pregnancy ,placenta ,rhino-pharyngeal swab ,placental swab ,Medicine (General) ,R5-920 - Abstract
Currently, limited data on maternal and neonatal outcomes of pregnant women with infection and pneumonia related to SARS coronavirus 2 (SARS-CoV-2) are available. Our report aims to describe a case of placental swabs positive for the molecular research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 RNA in an asymptomatic woman with positive rhino-pharyngeal swab for SARS-CoV-2 who underwent an urgent cesarean section in our obstetrics unit. Sample collection, processing, and laboratory testing were conducted in accordance with the World Health Organization (WHO) guidance. In the next months, conclusive data on obstetrical outcomes concerning the gestational age and pregnancy comorbidity as well as the eventual maternal–fetal transmission are needed.
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- 2020
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35. Risposte esatte ai Test di Verifica
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S. Bandini, M. Gallo, L. Caroti, N. Paudice, and L. Moscarelli
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Internal medicine ,RC31-1245 ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract non disponibile
- Published
- 2018
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36. Iperparatiroidismo ipercalcemico post-trapianto renale: un problema per il nefrologo
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S. Bandini, M. Gallo, L. Caroti, N. Paudice, and L. Moscarelli
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Iperparatiroidismo post-trapianto ,Ipercalcemia ,PTX ,Terapia con Calciomimetico ,Internal medicine ,RC31-1245 ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Descriviamo un caso di una paziente dializzata, sottoposta a paratiroidectomia pre-trapianto: la PTX non è stata risolutiva per mancato reperimento della IV ghiandola; a 6 mesi dall'intervento si è manifestata, infatti, una recidiva dell'iperparatiroidismo. Nel frattempo si è presentata la possibilità di eseguire un trapianto renale. Nonostante la “recidiva” dell'IPT, è stato deciso di optare per il trapianto renale che è stato effettuato con successo e con recupero precoce della funzione renale: si è manifestato, però, nel post-trapianto, un iperparatiroidismo residuale ipercalcemico. Di fronte al rischio di rendere aparatiroidea la paziente con una nuova PTX, si è optato per una terapia farmacologica. Per 6 anni la paziente trattata con calcitriolo (0,5–0,25 mcg a giorni alterni, con periodiche interruzioni dovute all'ipercalcemia) e difosfonati a cicli, ha mantenuto livelli di calcemia e di paratormone al di sopra dei valori di normalità senza raggiungere livelli di rischio, mentre il VFG si è mantenuto stabilmente nella norma. Nel dicembre 2008 a seguito di una frattura della branca ischio-pubica e per un progressivo incremento nell'ultimo anno dei livelli di calcemia e del PTH viene deciso di iniziare la somministrazione “off label” di Cinacalcet, di sospendere gradualmente lo steroide e di sostituire la ciclosporina con il Tacrolimus. Nei 3 anni di trattamento abbiamo notato, mantenendo costante la dose somministrata di Cinacalcet (30 mg/die), una riduzione significativa e persistente nel tempo dei livelli di calcemia e del PTH e un incremento della fosforemia. La funzione renale è persistita stabile senza episodi di rigetto. Indagini tomodensitometriche ripetute hanno rilevato un quadro di osteopenia sostanzialmente invariato. La nostra singola ma prolungata esperienza conferma in accordo con dati recenti della letteratura e in attesa dei risultati di uno studio RCT attualmente in corso, che questo farmaco può rappresentare una reale alternativa alla PTX mostrando grande efficacia e mancanza di effetti collaterali.
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- 2018
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37. Strategie preventive dell'infezione da Citomegalovirus nel trapianto di rene
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P. Carta, G. Rosso, N. Paudice, A. Larti, M. Zanazzi, L Moscatelli, L. Di Maria, and E. Bertoni
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Internal medicine ,RC31-1245 ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract non disponibile
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- 2018
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38. On the didactic function on the artist monograph
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Vincenzo Paudice
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Monografia ,Metodologia ,Storia dell'arte ,Storia della critica d'arte ,Fine Arts ,Arts in general ,NX1-820 - Abstract
In recent decades the debate on the "role" and "future" of the monograph in art and humanities research has flared up again in Europe, especially in France and England. Even though there are not homogenous positions, this debate shows how important it still is for the arts and humanities field to have a monograph approach. Particularly in history of art, this method is regarded as an "outil indispensable” - as Pierre Rosenberg writes in his introduction to the conference proceedings on Monographie d’artiste - in the academic path of the "future historien de l’art".The main focus of this paper is the artist monograph and how - mainly by virtue of both its tripartite structure and its “prosopopoeia” function - is the best methodological choice for students in their graduation thesis, in so far as it allows the greater acquisition of the necessary and specific knowledge and skills required in the field of the history of art.
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- 2017
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39. Legge come meta-prescrizione in Gilbert Ryle
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Lorenzo Paudice
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Philosophy (General) ,B1-5802 - Abstract
This paper examines the account of statute law as “impersonal injunction-ticket” sketched by Ryle in Ch. V of The Concept of Mind. For Ryle the traditional (mainly kantian) account of the general principles of Ethics and Law in terms of impersonal imperatives originates from a misunderstanding of their logical nature, as they constitute not individual prescriptions, but warrants and licences addressed to any potential giver of such prescriptions, abilitating him to emanate and enforce them. Ethical and juridical laws are therefore rules to give – respectively – behests and reproaches or injunctions and punishments, and consequently they are of a higher logical order of ad hominem prescriptions, as it happens in sciences to universal law-statements compared to the singular empirical statements satisfying them. Ryle’s construing of laws as meta-prescriptions cleanses their ontology from any metaphysical hypostatization (like his critics to the cartesian doctrine in philosophy of mind) and is close to his account of imagining, pretending and “obeying commands” as second-order performances. In the second half of the paper some seeming difficulties of such a construing are examined and possibly resolved discussing Ryle’s general views about Ethics, Law and Practical Reason in opposition to other traditional accounts of a more intellectualist kind.
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- 2017
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40. Conservative management of chronic kidney disease stage 5: role of angiotensin converting enzyme inhibitors
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Dattolo, Pietro C., Gallo, Pamela, Michelassi, Stefano, Paudice, Nunzia, Cannavò, Rossella, Romoli, Elena, Fani, Filippo, Tsalouchos, Aris, Mehmetaj, Alma, Ferro, Giuseppe, Sisca, Sergio, and Pizzarelli, Francesco
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- 2016
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41. Pseudo-designazioni ed entità immaginarie in G. Ryle
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Lorenzo Paudice
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Philosophy (General) ,B1-5802 - Abstract
This paper examines the analysis of inexistent entities offered by Ryle in his early essay Imaginary Objects (1933) and later more marginally in The Theory of Meaning (1957). For Ryle, as for most of the anglo-american philosophers of language in the Thirties, Russell’s Theory of Descriptions was the logical standard paradigm of analysis of proper names and descriptive singular expressions. In Imaginary Objects, its adoption is connected to an original elucidation of the act of imagining and the notion of “aboutness”, namely the sense in which can be said that a pseudo-designation (a description or proper name without reference) is about something.
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- 2016
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42. Successful Treatment of Refractory Wart with a Topical Activated Vitamin D in a Renal Transplant Recipient
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Luciano Moscarelli, Filomena Annunziata, Anduela Mjeshtri, Nunzia Paudice, Aris Tsalouchos, Maria Zanazzi, and Elisabetta Bertoni
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Surgery ,RD1-811 - Abstract
Warts are benign proliferations of the skin and mucosa caused by infection with human papillomavirus. They are commonly treated with destructive modalities such as cryotherapy with liquid nitrogen, local injection of bleomycin, electrocoagulation, topical application of glutaraldehyde, and local and systemic interferon-β therapy. These treatment modalities often cause pain and sometimes scarring or pigmentation after treatment. We herein report a case with a right index finger wart, which was successfully treated with a topical activated vitamin D.
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- 2011
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43. Inhibition by 5-HT3 receptor antagonists of release of cholecystokinin-like immunoreactivity from the frontal cortex of freely moving rats
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Raiteri, Maurizio, Paudice, Paolo, and Vallebuona, Francesco
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- 1993
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44. HISTOPATHOLOGICAL EVIDENCE OF COAL-WORKERS’ ANTHRACOFIBROSIS IN NATURAL MUMMIES OF 16TH-18TH CENTURIES.
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PAUDICE, MICHELE, TRAVERSARI, MIRKO, BIATTA, CHIARA MARIA, PEÑUELA, LEONARDO, BUFFELLI, FRANCESCA, SPINA, BRUNO, FULCHERI, EZIO, and VELLONE, VALERIO GAETANO
- Abstract
During the restoration works of the Conversion of St. Paul Church in Roccapelago (Modena, Italy, 2009), a burial crypt containing the remains of about 400 individuals was discovered. Natural mummification has occurred in 60 cases. Our Research Group, focused the paleopathological investigation on the pulmonary tract, since the reported massive coal dust exposure of Roccapelago’s dwellers even in parish records. In 24 mummies, the alleged pulmonary tissue was biopsied through preexisting solutions of continuity of the rib cage. In 18 cases (75%), lung tissue was recognized. In 7 cases (29,16%) we observed a massive deposition of black pigment, often nodular shaped, surrounded by dense fibrous tissue more evident with the Masson Trichrome staining. The morphological findings appeared diagnostics for anthracofibrosis secondary to massive pulmonary anthracosis. [ABSTRACT FROM AUTHOR]
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- 2021
45. Usefulness of Dermoscopy in Eruptive Syringomas in an Elderly Woman
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Capurro, Niccolò, Herzum, Astrid, Cozzani, Emanuele, Burlando, Martina, Riva, Silvia, Paudice, Michele, and Parodi, Aurora
- Abstract
Introduction:Eruptive syringomas (ES) are a rare variant of syringomas, benign adnexal tumors of eccrine sweat glands’ ducts. They mostly affect young-to middle-aged women, but rarely they may also occur in the elderly, requiring generally no specific treatment. Case Presentation:We present the case of a 76-year-old woman with sudden onset of ES. Clinical examination evidenced brown-to-orange papules and plaques on the anterior neck, corresponding dermatoscopically to orange-brownish structureless areas, with barely hinted peripheral incomplete network, superimposed on areas of light pink. Histology showed dermal proliferation of epithelial cells forming cords and ductules, confirming the clinical-dermoscopic suspect of ES. The lesions remained stable at 12-month follow-up without treatment. Discussion:This case highlights the role of dermoscopy to help differentiate ES from other clinically similar but more serious entities, such as histiocytosis, mastocytosis, and lichen planus, and to schedule the required confirmatory biopsy in due time without haste.
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- 2023
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46. Presynaptic Receptors Modulating Transmitter Release: Physiological and Pharmacological Aspects
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Raiteri, M., primary, Marchi, M., additional, Maura, G., additional, Bonanno, G., additional, Pittaluga, A., additional, Paudice, P., additional, Gemignani, A., additional, Pende, M., additional, and Drago, C., additional
- Published
- 1992
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47. PHARMACOLOGICALLY DISTINCT GABAB RECEPTOR SUBTYPES IN HUMAN AND RAT CNS
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Raiteri, M., Paudice, P., Gemignani, A., Fassio, A., Schmid, G., Sala, R., Carità, F., Chittolini, R., and Bonanno, G.
- Published
- 1997
48. Implementing NGS-based BRCAtumour tissue testing in FFPE ovarian carcinoma specimens: hints from a real-life experience within the framework of expert recommendations
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Rivera, Daniela, Paudice, Michele, Gismondi, Viviana, Anselmi, Giorgia, Vellone, Valerio Gaetano, and Varesco, Liliana
- Abstract
AimsNext Generation Sequencing (NGS)-based BRCAtumour tissue testing poses several challenges. As a first step of its implementation within a regional health service network, an in-house validation study was compared with published recommendations.MethodsEpithelial ovarian cancer (EOC) formalin-fixed paraffin-embedded specimens stored in the archives of the eight regional pathology units were selected from a consecutive series of patients with known BRCAgermline status. Two expert pathologists evaluated tumour cell content for manual macrodissection. DNA extraction, library preparation and NGS analyses were performed blinded to the germinal status. Parameters used in the study were confronted with guidelines for the validation of NGS-based oncology panels and for BRCAtumour tissue testing.ResultsNGS analyses were successful in 66 of 67 EOC specimens, with good quality metrics and high reproducibility among different runs. In all, 19 BRCApathogenic variants were identified: 12 were germline and 7 were somatic. A 100% concordance with blood tests was detected for germline variants. A BRCA1variant showed a controversial classification. In different areas of two early stage EOCs showing somatic variants, intratumour heterogeneity not relevant for test results (variant allele frequency >5%) was observed. Compared with expert recommendations, main limitations of the study were absence of controls with known somatic BRCAstatus and exclusion from the validation of BRCAcopy number variations (CNV).ConclusionsA close collaboration between pathology and genetics units provides advantages in the implementation of BRCAtumour tissue testing. The development of tools for designing and interpreting complex testing in-house validation could improve process quality.
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- 2021
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49. Gastric carcinoma in autoimmune gastritis: a histopathologic and molecular study
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Angerilli, Valentina, Vanoli, Alessandro, Celin, Giulia, Ceccon, Carlotta, Gasparello, Jessica, Sabbadin, Marianna, De Lisi, Giuseppe, Paudice, Michele, Lenti, Marco Vincenzo, Rovedatti, Laura, Di Sabatino, Antonio, Bazzocchi, Francesca, Lonardi, Sara, Savarino, Edoardo, Luchini, Claudio, Parente, Paola, Grillo, Federica, Mastracci, Luca, and Fassan, Matteo
- Abstract
Patients with autoimmune gastritis (AIG) have a 13-fold risk of developing type-1 neuroendocrine tumors, whereas the risk for gastric adenocarcinoma is still uncertain. Here we describe the clinicopathological and molecular features of a series of gastric carcinomas (GC) arising in the context of AIG. A total of 26 AIG-associated GC specimens were collected from four Italian Institutions. Immunohistochemistry for MUC1, MUC2, MUC5AC, MUC6, CDX2, HER2, PD-L1, CLDN18, Mismatch Repair (MMR) proteins, and p53 and EBER in situhybridization were performed. Histologic features and IHC were jointly reviewed by five expert gastrointestinal pathologists. Next-generation sequencing analysis (TrueSight Oncology 500, Illumina) of 523 cancer-related genes was performed on 19 cases. Most tumors were diagnosed as pT1 (52%), were located in the corpus/fundus (58%) and were associated with OLGA stage II gastritis (80.8%), absence of parietal cells, complete intestinal metaplasia and ECL-cell micronodular hyperplasia. Only 4 (15.4%) GCs were diagnosed during follow-up for AIG. The following histotypes were identified: 20 (77%) adenocarcinomas; 3 (11%) mixed neuroendocrine non-neuroendocrine neoplasms, and 2 (8%) high-grade solid adenocarcinomas with focal neuroendocrine component, 1 (4%) adenocarcinoma with an amphicrine component. Overall, 7 cases (27%) showed MMR deficiency, 3 (12%) were positive (score 3+) for HER2, 6 (23%) were CLDN18 positive, and 11 (42%) had PD-L1 Combined Positive Score ≥ 10. EBER was negative in all cases. Molecular analysis revealed 5/19 (26%) MSI cases and 7 (37%) TMB-high. The most frequently altered genes were: TP53(8/19, 42%), RNF43(7/19, 37%), ERBB2(7/19, 37% [two amplified and five mutated cases]), ARID1A(6/19, 32%), and PIK3CA(4/19, 21%). In summary, AIG-associated GCs are often diagnosed at low stage in patients with long-standing misrecognized severe AIG; they often display a neuroendocrine component or differentiation, have relatively higher rates of MMR deficiency, and TMB-high.
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- 2024
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50. The activation of phosphatidylinositol turnover is not directly involved in the modulation of neurotransmitter release mediated by presynaptic muscarinic receptors
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Marchi, Mario, Fontana, Giovanni, Paudice, Paolo, and Raiteri, Maurizio
- Published
- 1988
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