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Domesticated animals are artificially selected to exhibit desirable traits, however not all traits of domesticated animals are the result of deliberate selection. Loss of olfactory capacity in the domesticated pig (Sus scrofa domesticus) is one example. We used whole transcriptome analysis (RNA-Seq) to compare patterns of gene expression in the olfactory mucosa of the pig and two subspecies of wild boar (Sus scrofa), and investigate candidate genes that could be responsible for the loss of olfactory capacity. We identified hundreds of genes with reductions in transcript abundance in pig relative to wild boar as well as differences between the two subspecies of wild boar. These differences were detected mainly in genes involved in the formation and motility of villi, cilia and microtubules, functions associated with olfaction. In addition, differences were found in the abundances of transcripts of genes related to immune defenses, with the highest levels in continental wild boar subspecies. Overall, the loss of olfactory capacity in pigs appears to have been accompanied by reductions in the expression of candidate genes for olfaction. These changes could have resulted from unintentional selection for reduced olfactory capacity, relaxed selection for maintaining olfactory capacity, pleiotropic effects of genes under selection, or other non-selective processes. Our findings could be a cornerstone for future researches on wild boars, pigs, feral populations, and their evolutionary trajectories, aimed to provide tools to better calibrate species management as well as guidelines for breeders. [ABSTRACT FROM AUTHOR]

The need to find a trade-off between protecting water-related ecosystems and increasing safe water-use for human society is recognized in the 2030 Agenda of the European Union. We assess the ecological status of a riverine system in order to mitigate human impacts, considering its importance for supplying drinking water to more than 4 million users in Rome. We used an integrated approach, analyzing animal and plant communities at riverbanks and the riverbed. A macrobenthos analysis revealed a well-structured community with a good ecology for all sampling stations. The highest value was found immediately upstream and downstream of the springs collection system, while the lowest richness value was where the river collects urban wastewater. A floristic inventory showed Hemicryptophytes composing almost 45% of all species, and prevalence of Euroasiatic (35%) and Orophilous (34%) chorotypes. A positive correlation between riverbed vegetation and the quality of the benthic community was revealed, while tree height seems to have a negative trend. Our data suggest a river stretch affected by resurgence and water abstraction did not highlight irreversible alterations to the landscape. Indeed, the composition of vegetation and correlated animal communities mirrored a clinal gradient expected for an Apennine river system. Our study has the potential to improve the approach used to monitor the impacts of humans on freshwater ecosystems, aiming at preserving the integrity of the water-related landscape. [ABSTRACT FROM AUTHOR]