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Repeated viewing of the same video is a common strategy among autonomous language learners as well as a much used pedagogical strategy among foreign language (FL) teachers. Learners may watch the same video more than once, to increase global comprehension of the target language or to focus their attention on linguistic aspects, such as new vocabulary or pronunciation. This study sought to examine to what extent repetition is more efficient for vocabulary learning if the second viewing follows the first immediately, or a week later. Participants were upper intermediate-level college learners who were distributed into three groups, one watched a TV series episode twice in the same session, one also watched the same episode twice but one week apart, and the last (control) group did not watch the video. Tests of word meaning recognition and word meaning recall were administered before and after viewing (pretest-immediate posttest-delayed posttest). The pretest and posttests contained 23 target items and 17 distracters (single words and multi-word expressions). In addition, the study explored the influence of two learner factors, each related to one of two verbal input channels: sound recognition for the audio and reading efficacy (reading speed and comprehension) for the onscreen text. The results indicated significant vocabulary learning from viewing and slightly higher benefits for the spaced repetition group at immediate posttest. The results also showed a significant influence of previous target vocabulary knowledge and of aptitude, as measured by the LLAMA D test, but not of reading efficacy.

Submitting papers to conferences can be an effective way for early career researchers (ECRs) to gain exposure, receive feedback, create collaborations, and advance their careers in their field. However, top venues are extremely competitive, and require the submission of a high-quality abstract to be accepted to the conference. For instance, the conference of the European Second Language Association (EuroSLA) has an acceptance rate of around 50%, while its doctoral workshops (aimed specifically at ECRs undertaking doctoral research) have an acceptance rate of about 43%. This study sets out to facilitate early-career researchers’ journey to conference acceptance and to increase the quality of the submitted abstracts. We analysed 315 reviewer comments and identified fatal and non-fatal flaws. The results showed that 35% of variance in the rejection of abstracts was explained by flaws in such abstract sections as novelty, insufficient description of study design for its comprehension, clarity and style, research rationale, literature review, and feasibility and focus. This study provides implications and offers suggestions not only for ECRs wanting to apply for the EuroSLA doctoral workshops, but also for ECRs submitting abstracts to conferences more widely.

This study explores the effects of extensive audio-visual input with three captioning modes -- unenhanced captions, textually enhanced captions, and no captions -- on learning a variety of L2 grammatical constructions and examines the effects of three learnability factors: construction type, frequency, and recency. A total of 112 participants watched ten full-length TV series episodes over a period of five weeks. The study targeted 27 frequently occurring grammatical constructions categorized as fully-schematic, partially-filled, and fully-filled. The design included a pretest, an immediate posttest to measure the effects of recency, and a delayed posttest. The results indicated mixed effects of captioning: textually enhanced captions -- a more salient condition -- led to immediate learning outcomes while unenhanced captions resulted in higher long-term effects. A limit to the amount of different textually enhanced constructions presented in the input for effective learning is suggested. In general, unenhanced captions appear sufficient for successful grammar construction learning.

The present study explores the effects of distributed practice by extending this area of research to L2 learning from audiovisual input. A total of 96 L1 Russian elementary to advanced learners of English watched five episodes of captioned TV series under three viewing distribution conditions: longer spacing of viewing once per week; shorter spacing of viewing once per day; and massed binge-watching of viewing all the episodes in one session. The participants' knowledge of 25 target multi-word units was tested via the Vocabulary Knowledge Test before and after the intervention. The analyses considered learners' viewing conditions, proficiency level and feeling of learning as factors. The results indicated that the participants significantly improved their knowledge of the target multi-word units regardless of their viewing condition, but there was a benefit of longer spacing compared to the other two groups. Although binge-watching is an enjoyable everyday activity for many viewers, it might be advisable for L2 learners to space their viewing sessions to achieve better outcomes.

This study investigates the provision of two types of auditory elaborative automated digital feedback (metalinguistic and informational) in the context of a digital game for reading skills development in English as a foreign language. Nineteen 11-year-old Spanish-Catalan school children played through two digital minigames while their eye-movements were tracked. When they made an error, they received either metalinguistic or informational feedback. Analysing the gaze data from the eye-tracker alongside game log data about errors allowed us to compare how participants engaged cognitively with the two types of feedback. No differences were found in terms of which feedback type was better at helping students recover from their errors, however data showed that the two feedback types were processed differently. Evidence was found to suggest that those participants who listened to the informational feedback were able to utilise it more directly to recover from errors.

This article addresses the benefits of audio-visual input for learning second language (L2) vocabulary and grammatical constructions. Specifically, it explores the role of frequency, the effects of subtitles and captions, and the mediating role of learner proficiency on language gains in two longitudinal studies. Study 1 targets vocabulary acquisition in two groups of adolescents with an elementary L2 proficiency level who view 24 episodes of a TV series spread weekly over a whole academic year, one group with subtitles (first language) and one with captions (second language). Study 2 targets grammar acquisition in two groups of university students with an intermediate proficiency level who view 10 episodes over five weeks, one group with captions and one without captions. Results of both studies show significant correlations between language gains and frequency in the input, but the size of the frequency effect appears to depend on the type of support provided by the on-screen text. The analyses also show no significant advantage of captions or subtitles for vocabulary learning at this proficiency level, a significant advantage of captions over no captions for grammatical constructions learning, as well as the significant role of proficiency. It is concluded that viewing audio-visual material leads to L2 learning and it can support learners in their preparation for study abroad and maximize their learning experience during their sojourn.

Abstract Background Global health education partnerships should be collaborative and reciprocal to ensure mutual benefit. Utilisation of digital technologies can overcome geographic boundaries and facilitate collaborative global health learning. Global Health Classroom (GHCR) is a collaborative global health learning model involving medical students from different countries learning about each other’s health systems, cultures, and determinants of health via videoconference. Principles of reciprocity and interinstitutional partnership informed the development of the GHCR. This study explores learning outcomes and experiences in the GHCR between students from New Zealand and Samoa. Methods This study used a mixed methods approach employing post-GHCR questionnaires and semi-structured face-to-face interviews to explore self-reported learning and experiences among medical students in the GHCR. The GHCR collaboration studied was between the medical schools at the University of Otago, New Zealand and the National University of Samoa, Samoa. Results Questionnaire response rate was 85% (74/87). Nineteen interviews were conducted among New Zealand and Samoan students. Students reported acquiring the intended learning outcomes relating to patient care, health systems, culture, and determinants of health with regards to their partner country. Interview data was indicative of attitudinal changes in relation to cultural humility and curiosity. Some reported a vision for progress regarding their own health system. Students in the GHCR reported that learning with their international peers in the virtual classroom made learning about global health more real and tangible. The benefits to students from both countries indicated reciprocity. Conclusions This study demonstrates GHCR to be a promising model for collaborative and reciprocal global health learning using a student-led format and employing digital technology to create a virtual classroom. The self-reported learning outcomes align favourably with those recommended in the literature. In view of our positive findings, we present GHCR as an adaptable model for equitable, collaborative global health learning between students in internationally partnered institutions.

Many crop plants rely on insect pollination, particularly insect-pollinated crops which are functionally dioecious. These crops require insects to move pollen between separate plants which are functionally male or female. While honey bees are typically considered the most important crop pollinator species, many other insects are known to visit crops but the pollination contribution of the full diversity of these flower visitors is poorly understood. In this study, we examine the role of diverse insect pollinators for two kiwifruit cultivars as model systems for dioecious crops: Actinidia chinensis var. deliciosa ‘Hayward’ (a green-fleshed variety) and A. chinensis var. chinensis ‘Zesy002’ (a gold-fleshed variety). In our round-the-clock insect surveys, we identified that psychodid flies and mosquitoes were the second and third most frequent floral visitors after honey bees (Apis mellifera L), but further work is required to investigate their pollination efficiency. Measures of single-visit pollen deposition identified that several insects, including the bees Leioproctus spp. and Bombus spp. and the flies Helophilus hochstetteri and Eristalis tenax, deposited a similar amount of pollen on flowers as honey bees (Apis mellifera). Due to their long foraging period and high pollen deposition, we recommend the development of strategies to boost populations of Bombus spp., Eristalis tenax and other hover flies, and unmanaged bees for use as synergistic pollinators alongside honey bees.

Background. Pollinators, which provide the agriculturally and ecologically essential service of pollination, are under threat at a global scale. Habitat loss and homogenisation, pesticides, parasites and pathogens, invasive species, and climate change have been identified as past and current threats to pollinators. Actions to mitigate these threats, e.g., agri-environment schemes and pesticide-use moratoriums, exist, but have largely been applied post-hoc. However, future sustainability of pollinators and the service they provide requires anticipation of potential threats and opportunities before they occur, enabling timely implementation of policy and practice to prevent, rather than mitigate, further pollinator declines. Methods.Using a horizon scanning approach we identified issues that are likely to impact pollinators, either positively or negatively, over the coming three decades. Results.Our analysis highlights six high priority, and nine secondary issues. High priorities are: (1) corporate control of global agriculture, (2) novel systemic pesticides, (3) novel RNA viruses, (4) the development of new managed pollinators, (5) more frequent heatwaves and drought under climate change, and (6) the potential positive impact of reduced chemical use on pollinators in non-agricultural settings. Discussion. While current pollinator management approaches are largely driven by mitigating past impacts, we present opportunities for pre-emptive practice, legislation, and policy to sustainably manage pollinators for future generations.

Varroa destructor is a honey bee (Apis mellifera) parasite identified as one of the leading causes of overwintering colony loss in New Zealand. It has been shown that a naturally occurring heritable trait, "Varroa Sensitive Hygiene" (VSH), confers an advantage to colonies by increasing behaviours that limit the survival and reproduction of Varroa mites. The SNP 9-9224292 is an adenine/guanine (A/G) polymorphism on chromosome 9 of Apis mellifera where the G allele was observed to be associated with VSH behaviour in North American honey bees. In this study, we sought to determine if selection for the G allele of SNP 9-9224292 could decrease Varroa mite infestation of New Zealand honey bee (Apis mellifera ligustica) colonies. We genotyped queens and tracked their colonies over summer before measuring Varroa levels at the point of autumn Varroa treatment. The mean Varroa population level in colonies headed by queens that carry two copies of VSH associated G allele of SNP 9-9224292 was 28.5% (P

To protect New Zealand’s unique ecosystems and primary industries, imported plant materials must be constantly monitored at the border for high-threat pathogens. Techniques adopted for this purpose must be robust, accurate, rapid, and sufficiently agile to respond to new and emerging threats. Polymerase chain reaction (PCR), especially real-time PCR, remains an essential diagnostic tool but it is now being complemented by high-throughput sequencing using both Oxford Nanopore and Illumina technologies, allowing unbiased screening of whole populations. The demand for and value of Point-of-Use (PoU) technologies, which allow for in situ screening, are also increasing. Isothermal PoU molecular diagnostics based on recombinase polymerase amplification (RPA) and loop-mediated amplification (LAMP) do not require expensive equipment and can reach PCR-comparable levels of sensitivity. Recent advances in PoU technologies offer opportunities for increased specificity, accuracy, and sensitivities which makes them suitable for wider utilization by frontline or border staff. National and international activities and initiatives are adopted to improve both the plant virus biosecurity infrastructure and the integration, development, and harmonization of new virus diagnostic technologies.

The morphology of the adaxial structures of cones belonging to Palissya Endlicher 1847 emend. nov. are reinterpreted based on exquisitely preserved permineralised material from the Lower Cretaceous of Queensland. Although the material was not found in situ, it likely derives from the Orallo Formation, which is Valanginian in age. The cones have dual vascular bundles in each bract/scale complex, and the different tissue types in the bract and ovule/scale complex support interpretation of the cone as a compound structure. Since the early twentieth century it has been widely accepted that each ovule is surrounded by a cup-shaped structure, but the detailed morphology of the “cup” has hitherto been unclear. These new three-dimensionally preserved specimens with in situ ovules are described as Palissya tillackiorum sp. nov. This study demonstrates that the “cup” is formed from a pair of thin scales that subtend but are not fused to each ovule; each pair of scales comprises a thicker outer and thinner inner scale. The organographic relationships among ovules and scales in Palissya show a high degree of synorganisation. The adaxial surface of the bract/scale complex has 2–6 pairs of erect (orthotropous) ovules. The ovule/scale units are arranged symmetrically in two parallel rows on either side of the midline of the bract/scale. Individual ovule/scale units are comparable to those seen in extant Podocarpaceae and Taxaceae. The ovules are thin-walled and are interpreted to have a single integument and a non-thickened (non-lignified) micropyle. These new insights allow reinterpretation of material previously referred to Palissya. A new species is described from Yorkshire, England, as P. harrisii C.R. Hill ex Pattemore & Rozefelds sp. nov. All species based on well preserved cones are reconsidered herein: P. sphenolepis (Braun 1843) Nathorst 1908 emend. Florin 1958, P. elegans Parris, Drinnan & Cantrill 1995 emend. nov., P. bartrumii Edwards 1934 emend. nov., P. antarctica Cantrill 2000 and P. hunanensis Wang 2012. Palissya ovalis Parris et al. 1995 differs structurally from Palissya and is transferred to Knezourocarpon Pattemore 2000 emend. nov. Representatives of this genus may superficially resemble those of Palissya in compressions and impressions, and their congeneric status has been previously suggested; hence its inclusion in this study. Knezourocarpon has adaxial processes that are positioned in two parallel rows but it lacks ovules and paired lateral scales that formed a cup-shape, and its processes attach directly to a central vascular trace. The improved understanding of Palissya’s morphology allows for definite separation of these genera, although the higher-order affiliation of Knezourocarpon remains unclear.

High-throughput sequencing (HTS) of host plant small RNA (sRNA) is a popular approach for plant virus and viroid detection. The major bottlenecks for implementing this approach in routine virus screening of plants in quarantine include lack of computational resources and/or expertise in command-line environments and limited availability of curated plant virus and viroid databases. We developed: (1) virus and viroid report web-based bioinformatics workflows on Galaxy Australia called GA-VirReport and GA-VirReport-Stats for detecting viruses and viroids from host plant sRNA extracts and (2) a curated higher plant virus and viroid database (PVirDB). We implemented sRNA sequencing with unique dual indexing on a set of plants with known viruses. Sequencing data were analyzed using GA-VirReport and PVirDB to validate these resources. We detected all known viruses in this pilot study with no cross-sample contamination. We then conducted a large-scale diagnosis of 105 imported plants processed at the post-entry quarantine facility (PEQ), Australia. We detected various pathogens in 14 imported plants and discovered that de novo assembly using 21–22 nt sRNA fraction and the megablast algorithm yielded better sensitivity and specificity. This study reports the successful, large-scale implementation of HTS and a user-friendly bioinformatics workflow for virus and viroid screening of imported plants at the PEQ.

Background Neural mechanisms may play an important role in non-eosinophilic asthma (NEA). This study compared airway sensory nerve reactivity, using capsaicin challenge, in eosinophilic asthma (EA) and NEA and non-asthmatics.Methods Thirty-eight asthmatics and 19 non-asthmatics (aged 14–21 years) underwent combined hypertonic saline challenge/sputum induction, fractional exhaled nitric oxide, atopy and spirometry tests, followed by capsaicin challenge. EA and NEA were defined using a sputum eosinophil cut-point of 2.5%. Airway hyperreactivity was defined as a ≥15% drop in FEV1 during saline challenge. Sensory nerve reactivity was defined as the lowest capsaicin concentration that evoked 5 (C5) coughs.Results Non-eosinophilic asthmatics (n=20) had heightened capsaicin sensitivity (lower C5) compared with non-asthmatics (n=19) (geometric mean C5: 58.3 µM, 95% CI 24.1 to 141.5 vs 193.6 µM, 82.2 to 456.0; p

Mutualistic plant-pollinator interactions are critical for the functioning of both non-managed and agricultural systems. Mathematical models of plant-pollinator interactions can help understand key determinants in pollination success. However, most previous models have not addressed pollinator behavior and plant biology combined. Information generated from such a model can inform optimal design of crop orchards and effective utilization of managed pollinators like western honey bees (Apis mellifera), and help generate hypotheses about the effects of management practices and cultivar selection. We expect that the number of honey bees per flower and male to female flower ratio will influence fruit yield. To test the relative importance of these effects, both singly and simultaneously, we utilized a delay differential equation model combined with Latin hypercube sampling for sensitivity analysis. Empirical data obtained from historical records and collected in kiwifruit (Actinidia chinensis) orchards in New Zealand were used to parameterize the model. We found that, at realistic bee densities, the optimal orchard had 65-75% female flowers, and the most benefit was gained from the first 6-8 bees/1000 flowers, with diminishing returns thereafter. While bee density significantly impacted fruit production, plant-based parameters-flower density and male:female flower ratio-were the most influential. The predictive model provides strategies for improving crop management, such as choosing cultivars which have their peak bloom on the same day, increasing the number of flowers with approximately 70% female flowers in the orchard, and placing enough hives to maintain more than 6 bees per 1000 flowers to optimize yield.

Rapid and safe access to new plant genetic stocks is crucial for primary plant industries to remain profitable, sustainable, and internationally competitive. Imported plant species may spend several years in Post Entry Quarantine (PEQ) facilities, undergoing pathogen testing which can impact the ability of plant industries to quickly adapt to new global market opportunities by accessing new varieties. Advances in high throughput sequencing (HTS) technologies provide new opportunities for a broad range of fields, including phytosanitary diagnostics. In this study, we compare the performance of two HTS methods (RNA-Seq and sRNA-Seq) with that of existing PEQ molecular assays in detecting and identifying viruses and viroids from various plant commodities. To analyze the data, we tested several bioinformatics tools which rely on different approaches, including direct-read, de novo, and reference-guided assembly. We implemented VirusReport, a new portable, scalable, and reproducible nextflow pipeline that analyses sRNA datasets to detect and identify viruses and viroids. We raise awareness of the need to evaluate cross-sample contamination when analyzing HTS data routinely and of using methods to mitigate index cross-talk. Overall, our results suggest that sRNA analyzed using VirReport provides opportunities to improve quarantine testing at PEQ by detecting all regulated exotic viruses from imported plants in a single assay.

Avocado (Persea americana) has synchronously protogynous flowers: flowers open first in female phase before closing and opening the next day in male phase. Cultivars are grouped based on whether the flowers typically first open in female phase in the morning (type A), or in the afternoon (type B). However, it is known that environmental factors can alter the timing of flower opening, with cold temperatures being shown to affect the timing of flowering. The aim of this study was to investigate how low spring temperatures in New Zealand affect the flowering cycle of commercial avocado cultivars, focusing primarily on the receptive female phase of ‘Hass’, a type A cultivar. Time-lapse photography was used to assess flower opening times of ‘Hass’ over three years. Decreasing minimum overnight temperatures were associated with a delay in the timing of ‘Hass’ female flower phases and resulted in nocturnal flowering of both male and female phase flowers. We recorded insects visiting female flowers at night, and some nocturnal flower visitors collected were carrying avocado pollen. Our study suggests that nocturnal pollination needs to be considered for avocados grown in temperate regions. Furthermore, as the timing of the female phase of ‘Hass’ varied significantly with overnight temperature, the activity patterns of potential pollinators need to be considered to ensure adequate pollinator activity across the range of times in which ‘Hass’ flowers are receptive.

Umkomasiaceans are the most commonly recorded components of Gondwanan Triassic floras but they are not represented subsequently in that region. Their diversity appears to have substantially declined prior to the Rhaetian. Lower and Middle Jurassic floras in Australia and elsewhere in Gondwana are strikingly different from those of the Triassic, comprising ferns, lycopods, conifers, cycads, and bennettitaleans. The fern Cladophlebis Brongniart 1849 emend. Seward 1894 dominated coal-forming paludal environments during the Middle Jurassic in Queensland and it is one of the most commonly preserved plants in the Eastern Gondwanan Jurassic. Ginkgoaleans were present in Eastern Gondwana until near the end-Triassic but were absent below ca 60°S palaeolatitude during the Early and Middle Jurassic.

The plant fossil genera Umkomasia Thomas 1933 and Pteruchus Thomas 1933 emend. Townrow 1962 are known chiefly from the Middle and Upper Triassic of Gondwana. The structure of these fructifications has been conjectural, some being identified as pinnate, others as helically arranged. Specimens from the Ladinian-lower Norian of Queensland (northeastern Australia) show that the female and male fructifications - U. geminata (Shirley 1898) Rigby in Playford et al. 1982 emend. nov. and P. dubius Thomas 1933 emend. Townrow 1962, respectively - have a bipinnate structure. Those fructifications and the bipinnate leaf, Dicroidium feistmantelii (Johnston 1894) Gothan 1912, probably all belonged to the same parent plant. It was first suggested by John Townrow in 1962 that the sporangial heads of P. dubius have a pinnate structure; this character is confirmed herein. Pteruchus is recorded for the first time from the Carnian Tarong Basin, Queensland.

The plant megafossil genus Linguifolium Arber 1917 is chiefly known from the Middle and Upper Triassic of Gondwana. The range of Linguifolium extended beyond Gondwana by the Late Triassic, persisting there through the earliest Jurassic (Hettangian). The parent plants probably grew in a well-watered, canopied environment.

Cough is a common and troublesome symptom in people with asthma and is often associated with poorer asthma control and exacerbations. Apart from asthma, other causes or comorbidities might underlie cough in asthma, such as rhinosinusitis and bronchiectasis. Eosinophilic inflammation and bronchoconstriction can lead to an acute episode of cough or worsen chronic cough. Cough hypersensitivity with laryngeal paraesthesia, allotussia, and hypertussia might underlie the cough of asthma through augmented sensory nerve excitability of upper-airway vagal sensory nerves. Cough associated with bronchoconstriction and type 2 inflammation should respond to inhaled corticosteroids and long-acting β-adrenoceptor agonist therapy. For cough hypersensitivity in adults, speech and language therapy and neuromodulators (eg, gabapentin) could be considered. In children, there is no consistent association of asthma with cough sensitivity or between cough and asthma severity. Further research is needed to realise the potential of cough as a measure of asthma control, to understand the mechanisms of cough in asthma, and to develop safe, effective treatments and a precision-medicine approach to the management of cough in asthma in children and adults. [ABSTRACT FROM AUTHOR]

Approximately one-third of our food globally comes from insect-pollinated crops. The dependence on pollinators has been linked to yield instability, which could potentially become worse in a changing climate. Insect-pollinated crops produced via hybrid breeding (20% of fruit and vegetable production globally) are especially at risk as they are even more reliant on pollinators than open-pollinated plants. We already observe a wide range of fruit and seed yields between different cultivars of the same crop species, and it is unknown how existing variation will be affected in a changing climate. In this study, we examined how three hybrid carrot varieties with differential performance in the field responded to three temperature regimes (cooler than the historical average, average, and warmer that the historical average). We tested how temperature affected the plants' ability to set seed (seed set, pollen viability) as well as attract pollinators (nectar composition, floral volatiles). We found that there were significant intrinsic differences in nectar phenolics, pollen viability, and seed set between the carrot varieties, and that higher temperatures did not exaggerate those differences. However, elevated temperature did negatively affect several characteristics relating to the attraction and reward of pollinators (lower volatile production and higher nectar sugar concentration) across all varieties, which may decrease the attractiveness of this already pollinator-limited crop. Given existing predictions of lower pollinator populations in a warmer climate, reduced attractiveness would add yet another challenge to future food production.

Background Functional traits are the primary biotic component driving organism influence on ecosystem functions; in consequence, traits are widely used in ecological research. However, most animal trait-based studies use easy-to-measure characteristics of species that are at best only weakly associated with functions. Animal-mediated pollination is a key ecosystem function and is likely to be influenced by pollinator traits, but to date no one has identified functional traits that are simple to measure and have good predictive power. Methods Here, we show that a simple, easy to measure trait (hairiness) can predict pollinator effectiveness with high accuracy. We used a novel image analysis method to calculate entropy values for insect body surfaces as a measure of hairiness. We evaluated the power of our method for predicting pollinator effectiveness by regressing pollinator hairiness (entropy) against single visit pollen deposition (SVD) and pollen loads on insects. We used linear models and AICC model selection to determine which body regions were the best predictors of SVD and pollen load. Results We found that hairiness can be used as a robust proxy of SVD. The best models for predicting SVD for the flower species Brassica rapa and Actinidia deliciosa were hairiness on the face and thorax as predictors (R2 = 0.98 and 0.91 respectively). The best model for predicting pollen load for B. rapa was hairiness on the face (R2 = 0.81). Discussion We suggest that the match between pollinator body region hairiness and plant reproductive structure morphology is a powerful predictor of pollinator effectiveness. We show that pollinator hairiness is strongly linked to pollination—an important ecosystem function, and provide a rigorous and time-efficient method for measuring hairiness. Identifying and accurately measuring key traits that drive ecosystem processes is critical as global change increasingly alters ecological communities, and subsequently, ecosystem functions worldwide.

Background. Pollinators, which provide the agriculturally and ecologically essential service of pollination, are under threat at a global scale. Habitat loss and homogenisation, pesticides, parasites and pathogens, invasive species, and climate change have been identified as past and current threats to pollinators. Actions to mitigate these threats, e.g., agri-environment schemes and pesticide-use moratoriums, exist, but have largely been applied post-hoc. However, future sustainability of pollinators and the service they provide requires anticipation of potential threats and opportunities before they occur, enabling timely implementation of policy and practice to prevent, rather than mitigate, further pollinator declines. Methods.Using a horizon scanning approach we identified issues that are likely to impact pollinators, either positively or negatively, over the coming three decades. Results.Our analysis highlights six high priority, and nine secondary issues. High priorities are: (1) corporate control of global agriculture, (2) novel systemic pesticides, (3) novel RNA viruses, (4) the development of new managed pollinators, (5) more frequent heatwaves and drought under climate change, and (6) the potential positive impact of reduced chemical use on pollinators in non-agricultural settings. Discussion. While current pollinator management approaches are largely driven by mitigating past impacts, we present opportunities for pre-emptive practice, legislation, and policy to sustainably manage pollinators for future generations.

Tobacco exposure increases mortality and morbidity of the fetus, the child, the adolescent, and their children in turn. Nearly half the children in the world are exposed. Smoking is not merely personal choice or personal responsibility; those subtle phrases undermine those who have no choice in the matter.Tobacco control must take a multi-pronged attack. Smoking cessation by adults in childbearing years must take centre stage of these efforts, because it is the only way to ensure a smoke-free environment for children. Smoke-free parents provide a role model for smoke-free young people, and erode the image of smoking as a desirable adult behaviour to emulate. Pediatricians and pediatric pulmonologists have a key role to play here. This goal will reduce morbidity and mortality among adults and children. Legislation regarding taxation, environments, tobacco constituents, product placement and display, packaging, and media education are all key to this core goal. Smokefree policy must be protected from attack based on trade agreements.Research is needed into more effective ways to attract and help people give up smoking, and into educating and re-deploying tobacco industry workers in emerging and developed countries.