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101 results on '"Patruno, MARCO VINCENZO"'

6. Might the Masson trichrome stain be considered a useful method for categorizing experimental tendon lesions?

Author

Martinello, Tiziana, Pascoli, Francesco, Caporale, Giovanni, Perazzi, Anna, Iacopetti, Ilaria, and Patruno, MARCO VINCENZO

Subjects
Wound Healing, Sheep, Cumulative Trauma Disorders, Histological Techniques, Cell Count, Mesenchymal Stem Cells, Hindlimb, Tendons, Methyl Green, Tendon Injuries, Image Processing, Computer-Assisted, Animals, Eosine Yellowish-(YS), Collagen, Coloring Agents, Azo Compounds
Abstract

Strain injuries of tendons are the most common orthopedic injuries in athletic subjects, be they equine or human. When the tendon is suddenly damaged, an acute inflammatory phase occurs whereas its repetitive overloading may cause chronic injuries. Currently the criteria used for grading injuries are general and subjective, and therefore a reliable grading method would be an improvement. The main purpose of this study was to assess qualitatively the histological pattern of Masson trichrome stain in healthy and injured tendons; indeed, the known "paradox" of Masson staining was used to create an evaluation for the matrix of tendons, following experimental lesions and natural repair processes. A statistically significant difference of aniline-staining between healthy and lesioned tendons was observed. Overall, we think that the Masson staining might be regarded as an informative tool in discerning the collagen spatial arrangement and therefore the histological characteristics of tendons.

Published
2015

9. Vagus stimulation and brain ischemia, an interim report

Author

Patruno, MARCO VINCENZO, Denaro, Luca, D'Avella, Domenico, Gioffrè, Giorgio, Furlanis, Giulia Melinda, Martinello, Tiziana, Cavicchioli, Laura, Caporale, Giovanni, Lanmuller, Hermann, and Jarvis, Jonathan

Published
2015

25. Successful recellularization of human tendon scaffolds using adipose-derived mesenchymal stem cells and collagen gel

Author

Martinello, Tiziana, Bronzini, Ilaria, Volpin, Anna, Vindigni, Vincenzo, Maccatrozzo, Lisa, Caporale, Giovanni, Bassetto, Franco, and Patruno, MARCO VINCENZO

Subjects
Staining and Labeling, Tissue Scaffolds, Cell Survival, Mesenchymal Stem Cells, DNA, Cartilage Oligomeric Matrix Protein, Mesenchymal Stem Cell Transplantation, Collagen Type I, Tendons, Adipose Tissue, Humans, Regeneration, Colorimetry, Female, Collagen, Gels
Abstract

The major goal of regenerative medicine is to determine experimental techniques that take maximal advantage of reparative processes that occur naturally in the animal body. Injection of mesenchymal stem cells into the core of a damaged tendon represents such an approach. Decellularization of native tendons as potential targets and seeding protocols are currently under investigation. The aim of our study was to manufacture a recellularized biocompatible scaffold from cadaveric tissue for use in total or partial tendon injuries. Results showed that it was possible to introduce proliferating cells into the core of a decellularized tendon to treat the scaffold with a collagen gel. The method was effective in maintaining scaffold extracellular matrix and for expressing collagen type I and cartilage oligomeric matrix protein by injecting mesenchymal stem cells.

Published
2011

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