37 results on '"Patić, Aleksandra"'
Search Results
2. Diagnostic value of urine qRT-PCR for the diagnosis of West Nile virus neuroinvasive disease
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Cvjetković, Ivana Hrnjaković, Radovanov, Jelena, Kovačević, Gordana, Turkulov, Vesna, and Patić, Aleksandra
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- 2023
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3. Negative trend in seroprevalence of anti-toxoplasma gondii IgG antibodies among the general population of the province of Vojvodina, Serbia, 2008–2021
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Kovačević, Gordana, Cvjetković, Ivana Hrnjaković, Patić, Aleksandra, Radovanov, Jelena, and Kovačević, Branislav
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- 2023
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4. Analysis of Cultured Gut Microbiota Using MALDI-TOF MS in COVID-19 Patients from Serbia during the Predominance of the SARS-CoV-2 Omicron Variant.
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Patić, Aleksandra, Kovačević, Gordana, Vuković, Vladimir, Hrnjaković Cvjetković, Ivana, Ristić, Mioljub, Milosavljević, Biljana, Medić, Deana, Djilas, Milan, Radovanov, Jelena, Kovačević, Aleksandra, Pustahija, Tatjana, Balać, Dragana, and Petrović, Vladimir
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The currently dominant SARS-CoV-2 omicron variant, while causing mild respiratory symptoms, exhibits high transmissibility, drug resistance, and immune evasion. We investigated whether the presence of the SARS-CoV-2 affected the dynamics of fecal microbial composition isolated in culture in moderate COVID-19 patients. Blood, stool, and medical records were collected from 50 patients with confirmed SARS-CoV-2 infection. Two samples were taken per patient, at disease onset (within 5 days) and after symptom resolution (30–35 days). The part of the gut microbiota identifiable using MALDI-TOF MS was analyzed, and inflammatory cytokines and blood markers were measured in serum. The analysis identified 566 isolates at the species level, including 83 bacterial and 9 fungal species. Our findings indicate a change in the gut microbiota composition isolated in culture during the initial phase of infection, characterized by the proliferation of opportunistic bacteria such as Enterococcus spp. and Citrobacter spp., at the expense of beneficial commensal bacteria from the genus Bacillus and Lactobacillus. Additionally, the enrichment of fungal pathogens in fecal samples collected 30 days after the cessation of disease symptoms might suggest a prolonged disruption of the gut microbiota even after the resolution of COVID-19 symptoms. This study contributes to a growing body of evidence on the systemic effects of SARS-CoV-2 and highlights the importance of considering gastrointestinal involvement in the management and treatment of COVID-19. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Seroprevalence Study of Anti-HBs Antibodies in the General Population of Vojvodina, Serbia
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Rajčević, Smiljana, primary, Medić, Snežana, additional, Patić, Aleksandra, additional, Dragnić, Nataša, additional, Ristić, Mioljub, additional, Vuković, Vladimir, additional, and Petrović, Vladimir, additional
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- 2024
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6. Prevalence of oncogenic Human papillomavirus and genetic diversity in the L1 gene of HPV16 HPV 18 HPV31 and HPV33 found in women from Vojvodina Province Serbia
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Kovacevic, Gordana, Milosevic, Vesna, Knezevic, Petar, Knezevic, Aleksandra, Knezevic, Ivana, Radovanov, Jelena, Nikolic, Natasa, Patic, Aleksandra, Petrovic, Vladimir, Hrnjakovic Cvjetkovic, Ivana, and Stanisic, Ljiljana
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- 2019
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7. Seroepidemiology of Human Parvovirus B19 Infection among the Population of Vojvodina, Serbia, over a 16-Year Period (2008–2023).
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Vuković, Vladimir, Patić, Aleksandra, Ristić, Mioljub, Kovačević, Gordana, Hrnjaković Cvjetković, Ivana, and Petrović, Vladimir
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PARVOVIRUS B19 , *PARVOVIRUS diseases , *IMMUNOGLOBULINS , *MEDICAL personnel , *BLOOD diseases , *CHILDBEARING age , *IMMUNOGLOBULIN M - Abstract
This study aimed to estimate the serological status and dynamic changes in the prevalence of Parvovirus B19 (PVB19) antibodies within the general population residing in the northern part of the Republic of Serbia (Province of Vojvodina) during a 16-year period. Serum samples were analyzed for Human PVB19-specific IgM and IgG antibodies using enzyme-linked immunosorbent assay (ELISA). Throughout the study period, the overall seroprevalence was 49.51%. Approximately 10% of patients exhibited a serologic profile positive for PVB19 IgM antibodies. Notably, seroprevalence varied significantly, ranging from 9.12% in the pediatric cohort (ages 1–4 years) to 65.50% in the adult demographic (40–59 years old). Seroprevalence was higher (51.88%) among women compared to men (42.50%). Immunologically naive pregnant women in the age groups 26–36 and 36–45 years had 45% (OR = 0.55, 95% CI: 0.31–1.00) and 52% (OR = 0.48; 95% CI: 0.24–0.94) lower odds of having negative IgM and IgG compared to those in age group 16–25 years old. Improved knowledge of the epidemiology of PVB19 may assist clinicians in the differential diagnosis of PVB19 clinical manifestations. The PVB19 detection is particularly important for monitoring individuals in risk groups such as women of reproductive age, medical staff, patients with hematological disorders, and those with immunodeficiency. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Mosquito and human surveillance of mosquito-borne diseases in the Serbian city of Novi Sad in 2022
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Radovanov, Jelena, primary, Bijelović, Sanja, additional, Kovačević, Gordana, additional, Patić, Aleksandra, additional, Pustahija, Tatjana, additional, and Cvjetković, Ivana Hrnjaković, additional
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- 2023
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9. Motives and attitudes of parents toward HPV vaccination: Results from the initial period of HPV vaccine rollout in Serbia
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Štrbac, Mirjana, primary, Vuković, Vladimir, additional, Pustahija, Tatjana, additional, Nikolić, Nataša, additional, Rajčević, Smiljana, additional, Ilić, Svetlana, additional, Dugandžija, Tihomir, additional, Patić, Aleksandra, additional, Ristić, Mioljub, additional, and Petrović, Vladimir, additional
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- 2023
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10. Interleukin 6 in maternal serum as marker of bacterial infection and preterm delivery
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Erić Želimir, Patić Aleksandra, Bogavac Mirjana, and Tepić-Petrović Snežana
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interleukin-6 ,bacterial infections ,premature birth ,serologic tests ,Medicine - Abstract
Introduction: Preterm delivery remains a burning issue all over the world, especially in Serbia because of the rate of negative natural increase. IL-6 can stimulate the release of prostaglandins and cause premature contractions and premature labor. Aim of the Study: The aim of this research is to examine the importance of infection in the occurrence of premature contractions and to examine whether the preterm labor is associated with increased concentrations of IL-6 in patients with intact fetal membranes. Patients and Methods: We examined 83 pregnant women. The age range was between 15 and 43 years. The experimental group had 53 pregnant women and a control group was consisted of 30 pregnant women. All pregnant women had singleton pregnancies. The age of pregnancy in both groups was between the 21st and 35th week of gestation. The experimental group was divided into three groups according to the localization of infection: bacterial vaginosis, an infection of the cervix and urinary tract infections. The laboratory identification of IL-6 was performed as a double sandwich ELISA method. The reagents used for the identification of IL-6 were manufactured by Beckman-Coulter and were strictly intended for the research. Results: There were 34 pregnant women or 64% with infection of the cervix, 26 pregnant women or 49% had a positive urine culture, while bacterial vaginosis was present in 47 pregnant women or 89%. A positive finding on all localization was found in 14 pregnant women or 26%. In the experimental group, IL-6 was detected in 37 pregnant women or 70%. The mean value of IL-6 proven in the experimental group was 20.6 pg/ml (SD=18.2, n=53). Conclusion: This research demonstrated a direct link between a bacterial infection and preterm delivery. IL-6 can be used as a serological marker of bacterial infection and preterm delivery.
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- 2017
11. Human papillomavirus-specific antibody status among unvaccinated subjects in the region of Vojvodina, Serbia
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Kovačević, Gordana, primary, Božić Nedeljković, Biljana, additional, Patić, Aleksandra, additional, Radovanov, Jelena, additional, and Hrnjaković-Cvjetković, Ivana, additional
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- 2023
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12. Risk factors for severe influenza a virus infections in post-2009 pandemic period
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Radovanov Jelena, Hrnjaković-Cvjetković Ivana, Nikolić Nataša, Jovanović-Galović Aleksandra, Kovačević Gordana, Patić Aleksandra, Milošević Uroš, and Milošević Vesna
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influenza A ,acute respiratory infections ,real-time RT PCR ,Medicine - Abstract
Introduction. Literature data concerning risk factors for severe influenza in post-2009 pandemic period, from low- and middle-income Central and Eastern European countries are very limited. Objective. The aim of this study was to investigate the risk factors for severe A(H1N1)pdm09 and A(H3N2) influenza during the post-2009 pandemic period. Methods. During four consecutive seasons of 2010/2011-2013/2014, nasopharyngeal or nasal and pharyngeal swab samples from 153 patients with mild and 147 patients with severe influenza were tested using real-time reverse transcription polymerase chain reaction (real-time RT PCR) assays. Results. The study indicated three statistically significant risk factors of influenza severity, including presence of chronic underlying illness/condition [odds ratio (OR) of 15.2, 95% confidence interval (CI) of 1.8-125.4, p = 0.001), age ≥15 years (OR 9.2, 95% CI 3.5-24.1, p < 0.001), and delay in medical care of more than two days after the symptoms onset (OR 3.2, 95% CI 1.6-6.4, p = 0.001). Conclusion. Obtained results confirmed that patients with chronic underlying illness/condition and older than 15 years had the highest risk for serious complications from influenza and highlighted the importance of start of antiviral therapy within the first two days of illness in order to reduce the risk for the most severe outcomes of influenza, such as acute respiratory distress syndrome and lethal outcome.
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- 2016
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13. Immunogenicity of BNT162b2, BBIBP-CorV, Gam-COVID-Vac and ChAdOx1 nCoV-19 Vaccines Six Months after the Second Dose: A Longitudinal Prospective Study
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Petrović, Vladimir, primary, Vuković, Vladimir, additional, Patić, Aleksandra, additional, Marković, Miloš, additional, and Ristić, Mioljub, additional
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- 2022
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14. Seroepidemiology of SARS-CoV-2 Virus in Healthcare Workers before Circulation of the Omicron Sublineages BA.4/BA.5 in Vojvodina, Serbia
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Ristić, Mioljub, primary, Vuković, Vladimir, additional, Patić, Aleksandra, additional, Marković, Miloš, additional, and Petrović, Vladimir, additional
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- 2022
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15. Influenza B viruses in the population of province of Vojvodina during the 2012/2013 season: Differentiation of B/Yamagata and B/Victoria lineages by real-time RT-PCR, antigenic and phylogenetic characterization
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Radovanov Jelena, Milošević Vesna, Hrnjaković-Cvjetković Ivana, Ristić Mioljub, Đilas Milan, Nikolić Nataša, Patić Aleksandra, Kovačević Gordana, Jovanović-Galović Aleksandra, Petrović Tamaš, and Mikić Sandra Stefan
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influenza B ,acute respiratory infections ,epidemic ,real-time RT-PCR ,Medicine - Abstract
Introduction. At present, two phylogenetically distinct influenza B virus lineages, B/Yamagata and B/ Victoria, co-circulate worldwide and can cause significant morbidity and mortality. Objective. The aim of this study was to determine the prevalences of two influenza B virus lineages in the population of Vojvodina and to identify their antigenic and phylogenetic properties. Methods. A total of 369 and 334 nasopharyngeal, or nasal/throat swab samples, collected during the 2012/2013 and 2013/2014 seasons, respectively, were tested using specific singleplex influenza A, influenza B, influenza B/Yamagata and influenza B/Victoria real-time reverse transcription polymerase chain reaction (RT-PCR) assays. Antigenic and genetic testing were done by hemagglutination inhibition assay and hemagglutinin and neuraminidase gene sequence analysis, respectively. Results. During the 2012/2013 season, influenza B viruses were present in 53.4% (101/189) of influenza positive samples. The B/Yamagata-like viruses (81.2%) significantly predominated over the B/Victoria-like viruses (18.8%). Comparing to B/Victoria-like positive patients, among B/Yamagata-like positive patients, children 5-14 years of age were significantly more represented (5.3% vs. 35.4%, respectively), as well as patients with mild form of illness (15.8% vs. 45.1%, respectively). The results of sequence analysis and antigenic testing showed that tested viruses were not closely related to B/Wisconsin/1/2010, the vaccine virus for 2012/2013. During the 2013/2014 season influenza B viruses were not detected. Conclusion. The results of this study confirmed the health significance of influenza B viruses and indicated that B/Yamagata-like viruses were significantly more prevalent than B/Victoria lineage viruses, during the 2012/2013 season. They also showed a sub-optimal match between the tested viruses and the vaccine virus for season 2012/2013. [Projekat Ministarstva nauke Republike Srbije, br. TR31084]
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- 2015
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16. Detection and Molecular Characterization of Rotavirus Infections in Children and Adults with Gastroenteritis from Vojvodina, Serbia
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Patić, Aleksandra, primary, Vuković, Vladimir, additional, Kovačević, Gordana, additional, Petrović, Vladimir, additional, Ristić, Mioljub, additional, Djilas, Milan, additional, Knežević, Petar, additional, Pustahija, Tatjana, additional, Štrbac, Mirjana, additional, Djekić Malbaša, Jelena, additional, Rajčević, Smiljana, additional, and Hrnjaković Cvjetković, Ivana, additional
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- 2022
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17. Toxoplasmosis in children of the South Bačka region, Serbia: A new light in the public health perspective
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Jovanović-Galović Aleksandra, Milošević Vesna, Hrnjaković-Cvjetković Ivana, Kovačević Gordana, Radovanov Jelena, Elez Ivana, and Patić Aleksandra
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Toxoplasma gondii ,children ,public health ,seroprevalence ,Biology (General) ,QH301-705.5 - Abstract
In this study the presence of anti-Toxoplasma IgM and IgG antibodies in sera of 245 children, aged 1 to 18 years, was determined. In the eldest group (15-18), 29.2% of the sera were positive for the IgG antibody. Toxoplasma gondii infection in pregnant women and immunosuppressed patients produces severe consequences, while in immunocompetent individuals it is considered asymptomatic and latent. Recent emerging links to mental disorders has shed a completely new light on the public health view of this common infection. It calls for a new approach in the prevention and screening of a population, children being the target group. [Projekat Ministarstva nauke Republike Srbije, br. TR31084]
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- 2014
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18. Epidemiological study on the incidence of haemorrhagic fever with renal syndrome in five Western Balkan countries for a 10-year period: 2006–2015
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Štrbac, Mirjana, Vuković, Vladimir, Patić, Aleksandra, Medić, Snežana, Pustahija, Tatjana, Petrović, Vladimir, Lendak, Dajana, Kosanović Ličina, Mirjana, Bakić, Marijan, Protić, Jelena, Pranjić, Nurka, Jandrić, Ljubica, Sokolovska, Nikolina, Ristić, Mioljub, Štrbac, Mirjana, Vuković, Vladimir, Patić, Aleksandra, Medić, Snežana, Pustahija, Tatjana, Petrović, Vladimir, Lendak, Dajana, Kosanović Ličina, Mirjana, Bakić, Marijan, Protić, Jelena, Pranjić, Nurka, Jandrić, Ljubica, Sokolovska, Nikolina, and Ristić, Mioljub
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Background: Large-scale epidemics of haemorrhagic fever with renal syndrome (HFRS) have been reported mostly in Asia and Europe, with around 100,000 people affected each year. In the Southeast Europe, Balkan region, HFRS is endemic disease with approximately 100 cases per year. Our aim was to describe epidemiological characteristics of HFRS in five Western Balkan (WB) countries and to describe correlation between HFRS incidence and major meteorological event that hit the area in May 2014. Methods: National surveillance data of HFRS from Bosnia and Herzegovina, Croatia, Montenegro, North Macedonia and Serbia obtained from 1 January 2006 to 31 December 2015 were collected and analysed. Results: In a 10-year period, a total of 1,065 HFRS patients were reported in five WB countries. Cumulative incidence rate ranged from 0.05 to 15.80 per 100.000 inhabitants (in North Macedonia and Montenegro respectively). Increasing number of HFRS cases was reported with a peak incidence in three specific years (2008, 2012, and 2014). Average incidence for the entire area was higher in males than females (5.63 and 1.90 per 100.000 inhabitants respectively). Summer was the season with the highest number of cases and an average incidence rate of 1.74/100.000 inhabitants across 10-year period. Haemorrhagic fever with renal syndrome incidence was significantly increased (7.91/100.000 inhabitants) in 2014, when a few months earlier, severe floods affected several WB countries. A strong significant negative correlation (r = −.84, p < .01) between the monthly incidence of HFRS and the number of months after May's floods was demonstrated for the total area of WB. Conclusion: Our findings demonstrate that the HFRS incidence had similar distribution (general, age, sex and seasonality) across majority of the included countries. Summer was the season with the highest recorded incidence. Common epidemic years were detected in all observed countries as well as a negative correlation between the mont
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- 2022
19. Immunogenicity of BNT162b2, BBIBP-CorV and Gam-COVID-Vac vaccines and immunity after natural SARS-CoV-2 infection—A comparative study from Novi Sad, Serbia
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Petrović, Vladimir, primary, Vuković, Vladimir, additional, Patić, Aleksandra, additional, Marković, Miloš, additional, and Ristić, Mioljub, additional
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- 2022
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20. Epidemiological study on the incidence of haemorrhagic fever with renal syndrome in five Western Balkan countries for a 10‐year period: 2006–2015
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Štrbac, Mirjana, primary, Vuković, Vladimir, additional, Patić, Aleksandra, additional, Medić, Snežana, additional, Pustahija, Tatjana, additional, Petrović, Vladimir, additional, Lendak, Dajana, additional, Ličina, Mirjana Kosanović, additional, Bakić, Marijan, additional, Protić, Jelena, additional, Pranjić, Nurka, additional, Jandrić, Ljubica, additional, Sokolovska, Nikolina, additional, and Ristić, Mioljub, additional
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- 2022
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21. Epidemiological characteristics of COVID-19 travel-associated cases in Vojvodina, Serbia, during 2020
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Pustahija, Tatjana, primary, Ristić, Mioljub, additional, Medić, Snežana, additional, Vuković, Vladimir, additional, Štrbac, Mirjana, additional, Rajčević, Smiljana, additional, Patić, Aleksandra, additional, and Petrović, Vladimir, additional
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- 2021
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22. Immunogenicity of BNT162b2, BBIBP-CorV, Gam-COVID-Vac and ChAdOx1 nCoV-19 Vaccines Six Months after the Second Dose: A Longitudinal Prospective Study.
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Petrović, Vladimir, Vuković, Vladimir, Patić, Aleksandra, Marković, Miloš, and Ristić, Mioljub
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COVID-19 vaccines ,IMMUNE response ,GENDER differences (Sociology) ,VACCINES ,HUMORAL immunity - Abstract
Many available SARS-CoV-2 vaccines demonstrated good humoral response, but studies directly comparing their immunogenicity in the general population are lacking. We evaluated the medium–term kinetics of anti-S SARS-CoV-2 antibodies (Abs) at one and six months after the second dose of BNT162b2, BBIBP-CorV, and Gam-COVID-Vac. Immunogenicity at six months was directly compared between BNT162b2, BBIBP-CorV, Gam-COVID-Vac, and ChAdOx1 nCoV-19. Participants ≥ 20 years old from Novi Sad, Serbia, without prior SARS-CoV-2 infection, were included. Anti S1/S2 IgG antibodies were measured using quantitative LIAISON SARS-CoV-2 assay. A total of 368 participants were included: 231 (62.77%) had sera collected at two time points. Two doses of BNT162b2 were received by 37.50% of participants, followed by BBIBP-CorV (22.01%), Gam-COVID-Vac (21.47%), and ChAdOx1 nCoV-19 (19.02%). Mean Ab levels at the 28th day and 6 months were 216.55 (SD = 105.73) AU/mL and 75.68 (SD = 57.30) for BNT162b2, 194.38 (SD = 140.24) and 90.53 (SD = 111.30) for Gam-COVID-Vac, and 72.74 (SD = 80.04) and 24.43 (SD = 38.43) for BBIBP-CorV group (p < 0.01, between two time points across all three groups), with a significant difference between women and men (p < 0.01, for both sexes). At the sixth month post-vaccination, the highest mean Ab level was detected in Gam-COVID-Vac group (91.28 AU/mL, SD = 95.96), followed by BNT162b2 (85.25 AU/mL, SD = 60.02), ChAdOx1 nCoV-19 (64.22 AU/mL, SD = 65.30), and BBIBP-CorV (25.26 AU/mL, SD = 36.92) (p < 0.01). Anti-spike IgG persistence was demonstrated six months post-vaccination with a significant decline in Ab levels. These results suggest a lower protection against SARS-CoV-2 over time. Our findings support the introduction of additional (booster) doses. [ABSTRACT FROM AUTHOR]
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- 2023
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23. Seroepidemiological study of rubella in Vojvodina, Serbia: 24 years after the introduction of the MMR vaccine in the national immunization programme
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Patić, Aleksandra, primary, Štrbac, Mirjana, additional, Petrović, Vladimir, additional, Milošević, Vesna, additional, Ristić, Mioljub, additional, Hrnjaković Cvjetković, Ivana, additional, and Medić, Snežana, additional
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- 2020
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24. Značaj molekularne dijagnostike u dokazivanju virusnog gastrointestinalnog sindroma u Vojvodini
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Patić, Aleksandra, Milošević, Vesna, Hrnjaković-Cvjetković, Ivana, Knežević, Petar, Petrović, Vladimir, Petrović, Tamaš, and Knežević, Aleksandra
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Rotavirus ,Gastroenteritis + virology ,Norovirus ,Astrovirus ,Adenoviruses, Human ,Molecular Diagnostic Techniques ,Genome ,Adenoviruses ,gastroenteritis + virusologija ,rotavirus ,norovirus ,astrovirus ,humani adenovirusi ,molekularne dijagnostičke metode ,genom ,Human - Abstract
Uvod: Virusni gastrointestinalni sindrom je aktuelni zdravstveni problem u celom svetu. To važi kako u razvijenim zemljama, tako i u zemljama u razvoju, a posebno u nerazvijenim zemljama, gde je drugi po redu uzrok mortaliteta. Nagli početak bolesti, praćen pojavom velikog broja tečnih stolica, mukom, povraćanjem, bolovima u stomaku, temperaturom, malaksalošću, ima za posledicu dehidrataciju. U svim starosnim grupama obolelih, a naročito kod sasvim male dece, starih i imunodeficitarnih osoba može da dođe do smrtnog ishoda, ukoliko se brzo ne postavi tačna etiološka dijagnoza bolesti i ne pristupi se odmah nadoknadi vode i elektrolita, kao i primeni svih ostalih mera simptomatske terapije. Brzo postavljena tačna dijagnoza, što se najbolje postiže real-time PCR testom, sprečava pojavu komplikacija, pa i fatalnog ishoda bolesti. Istovremeno, omogućava primenu odgovarajućih epidemioloških mera da se spreči nastanak epidemija i njihovo širenje. Cilj ovog istraživanja bio je da se tačno utvrdi incidenca virusnog gastrointestinalnog sindroma u Vojvodini i učestalost pojave epidemijskog i sporadičnog javljanja ove bolesti. Cilj je bio i postavljanje algoritma za primenu real-time PCR testa u dijagnostici virusnog gastrointestinalnog sindroma u budućem radu. Isto tako, cilj je bio da se molekularnom analizom, sekvenciranjem delova genoma pozitivnih uzoraka stolice, izvrši genetska tipizacija i odredi filogenetska pripadnost virusa. Materijal i metode: Tokom petogodišnjeg istraživanja molekularnim real-time PCR testom pregledane su 1003 obolele osobe sa simptomima virusnog dijarealnog sindroma, starosti od mesec dana do preko 90 godina. Pregledani su na rota, noro, astro i enterične adenoviruse. Na osnovu podataka iz anketnih upitnika i istorija bolesti, detaljno su analizirani svi klinički pokazatelji (javljanje bolesti tokom godine, trajanje bolesti, simptomi). Procena težine kliničke slike vršena je prema Vesikari skali. Svi podaci su upoređivani prema vrsti virusnog uzročnika, prema starosti obolelih, godinama trajanja istraživanja i epidemijskom i sporadičnom javljanju oboljenja. Dobijeni podaci su statistički obrađeni, tabelarno i grafički prikazani. Rezultati: U petogodišnjem periodu real-time PCR testom pregledan je uzorak od 1003 obolele osobe različite starosti na 4 virusna uzročnika dijarealnog sindroma (rota, noro, astro i enterične adenoviruse). Virusni dijarealni sindrom dokazan je kod 709 obolelih (70,69%). Najčešće su dokazane rotavirusne infekcije u 28,81%. Statistički značajno najčešće rotavirusi su bili utvrđeni kod dece do 5 godina (38,90%), ali u visokom procentu i kod dece uzrasta 6 do 14 godina (24,83%). Deca mlađa od 5 godina imala su statistički značajno najtežu kliničku sliku, bila su češće hospitalizovana i imala su statistički značajno višu temperaturu. Pored više temperature kod obolelih od rotavirusa, klinička slika je kod ovih bolesnika bila teža i bolest je duže trajala nego kod obolelih od drugih virusa. Norovirusna infekcija je dokazana u 23,03% obolelih i to statistički značajno češće kod odraslih osoba, starijih od 20 godina. Od kliničkih simptoma kod ovih bolesnika statistički značajno češće su dokazani muka, povraćanje i bolovi u stomaku, nego kod obolelih od drugih virusa. Norovirusi su značajno češće bili uzročnici epidemijskog javljanja bolesti. Astrovirus je dokazan kod znatno manjeg broja obolelih (u 2,29%) i to samo kod dece do 5 godina i dece uzrasta 6 do 14 godina. Infekcija izazvana enteričnim adenovirusima dokazana je kod 13,36% bolesnika. Njačešće je utvrđena kod dece uzrsta do 5 godina i 6 do 14 godina. Oboleli od adenovirusa imali su statistički značajno blažu kliničku sliku bolesti. Dva virusna uzročnika u uzorku stolice dokazana su u 3,19% osoba, obično u toku epidemijskog javljanja bolesti. Ovi bolesnici su imali bitno težu kliničku sliku. Najviše obolelih od dijarealnog sindroma bilo je u hladnim mesecima, mada su dijagnostikovani i tokom cele godine. U petogodišnjem periodu utvrđene su 22 epidemije u kolektivima i 9 porodičnih epidemija. Epidemijsko javljanje bolesti bilo je statistički značajno najčešće kod najstarijih bolesnika (starijih od 50 godina), a sporadično javljanje bilo je statističko značajno najčešće kod dece. U cilju potvrde tačnosti dijagnostike virusa u ispitivanim uzorcima real-time PCR testom, genotipizacije, kao i detaljnije molekularne analize, izabrani su reprezentativni uzorci pozitivni na rota, noro, astro ili adenoviruse. Delovi genoma ovih uzoraka su amplifikovani, a zatim sekvencirani. Sekvencirani izolati rotavirusa pripadali su grupi A i tipovima G1P[8], G2P[4], G3P[8] i G9P[8]. Sekvencirani izolati norovirusa pripadali su genogrupi I tipu 2, zatim genogrupi II tipovima 1, 2, 4 i 17. Sekvencirani izolati astrovirusa pripadali su grupi klasičnih astrovirusa i tipovima 1, 4 i 5. Sekvencirani izolati adenovirusa pripadali su grupi F i tipovima 40 i 41, kao i grupi C tipu 2. Pripadnost dobijenih sekvenci u ovom istraživanju, dodatno je potvrđena izradom filogenetskog stabla za sekvence pozitivne na rota, noro, astro ili adenoviruse. Zaključak: Incidenca virusnog dijarealnog sindroma u Vojvodini (70,69%) vrlo je visoka i viša je nego što je bilo pretpostavljeno prilikom prijave teze (u hipotezi). Real-time PCR test treba da bude redovno korišćen u budućem dijagnostičkom radu, jer dovodi do brze dijagnostike, čak i ako su virusi prisutni u malom broju u uzorcima tečnih stolica, što je utvrđeno tokom ovog dijagnostičkog rada. Ispitivani virusi treba da budu redovno dijagnostikovani kod obolelih od dijarealnog sindroma i to u svim starosnim grupama, tokom epidemijskog i sporadičnog javljanja oboljenja., Introduction: Viral gastrointestinal syndrome is a current ongoing health problem worldwide. This is true of both developed and developing countries, especially underdeveloped ones where it is the second leading cause of mortality. Sudden onset of the disease—accompanied by the occurrence of large numbers of liquid stools, nausea, vomiting, abdominal pain, fever, and exhaustion—leads to dehydration. A fatal outcome can occur in all age groups of patients, especially very young children, the elderly, and the immuno-deficient, unless an accurate etiological diagnosis of the disease is quickly established, followed by a prompt institution of fluid and electrolyte placement, and implementation of other symptomatic therapy measures. Quick establishment of an accurate diagnosis, which is best achieved using the real-time PCR test, prevents the onset of complications, including a potentially fatal outcome of the disease. Simultaneously, it enables the implementation of appropriate epidemiological measures to prevent epidemic outbreaks and their spread. The aim of this study was to accurately determine the incidence of viral gastrointestinal syndrome in Vojvodina and the frequency of epidemic and sporadic occurrence of this disease. The aim was also to set up an algorithm for the application of the real-time PCR test in diagnostics of viral gastrointestinal syndrome in future work. Likewise, the aim was to carry out genetic typing and determine phylogenetic affiliation of the virus using molecular analysis and sequencing of parts of genomes from positive stool samples. Material and Methods: During a five-year study, 1003 patients with symptoms of viral diarrheal syndrome, aged from one month to more than 90 years old, were examined using molecular real-time PCR test. They were screened for rota, noro, astro, and enteric adenoviruses. Based on the data from survey questionnaires and medical case history, all clinical indicators were meticulously analyzed (disease occurrence during the year, disease duration, symptoms). The assessment of the clinical severity was carried out according to the Vesikari Clinical Severity Scoring scale. All data were compared according to the type of the viral causing agent, age of the patients, duration of research in years, and epidemic and sporadic occurrence of the disease. Obtained data were statistically analyzed, tabulated, and graphically displayed. Results: In a five-year period, a sample of 1003 patients of different ages was screened for four different viral causing agents of diarrheal syndrome (rota, noro, astro, and enteric adenoviruses) using the real-time PCR test. Viral diarrheal syndrome was confirmed in 709 patients (70.69%). The most commonly found were rotavirus infections in 28.81% of the cases. Rotaviruses were statistically significantly most common in children younger than 5 years old (38.90%), but were also found in high percentage in children aged 6-14 years old (24.83%). Children under 5 years of age had statistically significantly highest clinical severity and fever, and were more frequently hospitalized. In addition to higher fever in patients with rotavirus, clinical severity in these patients was also higher, and the disease lasted longer than in patients with other viruses. Norovirus infections were reported in 23.03% of the subjects, statistically significantly more frequently in adults over 20 years of age. Regarding the clinical symptoms in these patients, nausea, vomiting, and abdominal pain were statistically significantly more common than in patients with other viruses. Noroviruses were significantly more common as causing agents of epidemic disease outbreaks. Astrovirus was found in a significantly smaller number of patients (in 2.29%), and only in children under 5 years of age and children aged 6-14 years old. Enteric adenovirus infections were reported in 13.36% of the subjects. They were most commonly found in children younger than 5, and those aged 6- 14 years old. Adenovirus sufferers had statistically significantly milder clinical disease. Two viral causing agents in the stool sample were found in 3.19% of the subjects, usually during an epidemic disease outbreak. These patients had a significantly more severe clinical disease. Highest numbers of sufferers from diarrheal syndrome occurred during the cold months, although they were diagnosed throughout the year. In a five-year period, 22epidemics in collective groups and 9 family epidemics were identified. Epidemic outbreaks of the disease were statistically significantly most frequent in the elderly patients (older than 50), while sporadic occurrences were statistically significantly most frequent in children. Representative samples positive for rota, noro, astro, or adenoviruses were selected in order to confirm the accuracy of virus diagnostics in samples tested by the real-time PCR test, and perform genotyping as well as more detailed molecular analyses. Parts of the genomes of these samples were amplified and then sequenced. Sequenced rotavirus isolates belonged to group A and types G1P[8], G2P[4], G3P[8], and G9P[8]. Sequenced norovirus isolates belonged to genogroup I type 2, and genogroup II types 1, 2, 4, and 17. Sequenced astrovirus isolates belonged to the group of classical astroviruses and types 1, 4, and 5. Sequenced adenovirus isolates belonged to group F and types 40 and 41, as well as group C type 2. The affiliation of the obtained sequences in this study was further confirmed by creating a phylogenetic tree for sequences positive for rota, noro, astro, or adenoviruses. Conclusion: The incidence of viral diarrheal syndrome in Vojvodina (70.69%) is very high—higher than what was assumed at the time of the thesis submission (in the hypothesis). The real-time PCR test should be regularly used in future diagnostic work, since it leads to rapid diagnostics even if viruses are present in small numbers in liquid stool samples, as determined in the course of this diagnostic study. The investigated viruses should be regularly tested in patients with diarrheal syndrome belonging to all age groups during both epidemic and sporadic occurrences of the disease.
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- 2018
25. USUTU VIRUS: AN EMERGING FLAVIVIRUS IN EUROPE
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Hrnjaković Cvjetković, Ivana, primary, Petrović, Tamaš, additional, Petrić, Dušan, additional, Milošević, Uroš, additional, Radovanov, Jelena, additional, Kovačević, Gordana, additional, Jovanović Galović, Aleksandra, additional, Patić, Aleksandra, additional, Nikolić, Nataša, additional, Cvjetković, Dejan, additional, Stefan Mikić, Sandra, additional, and Milošević, Vesna, additional
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- 2019
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26. SEROPREVALENCE OF INFECTION WITH TOXOPLASMA GONDII AMONG THE RESIDENTS OF SOUTH BACKA DISTRICT, SERBIA
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Hrnjaković Cvjetković, Ivana, primary, Milošević, Vesna, additional, Petrović, Tamaš, additional, Petrić, Dušan, additional, Radovanov, Jelena, additional, Kovačević, Gordana, additional, Patić, Aleksandra, additional, Nikolić, Nataša, additional, Dopudj, Nela, additional, Stefan, Sandra, additional, and Cvjetković, Dejan, additional
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- 2019
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27. Značaj molekularne dijagnostike u dokazivanju virusnog gastrointestinalnog sindroma u Vojvodini
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Milošević, Vesna, Hrnjaković-Cvjetković, Ivana, Knežević, Petar, Petrović, Vladimir, Petrović, Tamaš, Knežević, Aleksandra, Patić, Aleksandra, Milošević, Vesna, Hrnjaković-Cvjetković, Ivana, Knežević, Petar, Petrović, Vladimir, Petrović, Tamaš, Knežević, Aleksandra, and Patić, Aleksandra
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Uvod: Virusni gastrointestinalni sindrom je aktuelni zdravstveni problem u celom svetu. To važi kako u razvijenim zemljama, tako i u zemljama u razvoju, a posebno u nerazvijenim zemljama, gde je drugi po redu uzrok mortaliteta. Nagli početak bolesti, praćen pojavom velikog broja tečnih stolica, mukom, povraćanjem, bolovima u stomaku, temperaturom, malaksalošću, ima za posledicu dehidrataciju. U svim starosnim grupama obolelih, a naročito kod sasvim male dece, starih i imunodeficitarnih osoba može da dođe do smrtnog ishoda, ukoliko se brzo ne postavi tačna etiološka dijagnoza bolesti i ne pristupi se odmah nadoknadi vode i elektrolita, kao i primeni svih ostalih mera simptomatske terapije. Brzo postavljena tačna dijagnoza, što se najbolje postiže real-time PCR testom, sprečava pojavu komplikacija, pa i fatalnog ishoda bolesti. Istovremeno, omogućava primenu odgovarajućih epidemioloških mera da se spreči nastanak epidemija i njihovo širenje. Cilj ovog istraživanja bio je da se tačno utvrdi incidenca virusnog gastrointestinalnog sindroma u Vojvodini i učestalost pojave epidemijskog i sporadičnog javljanja ove bolesti. Cilj je bio i postavljanje algoritma za primenu real-time PCR testa u dijagnostici virusnog gastrointestinalnog sindroma u budućem radu. Isto tako, cilj je bio da se molekularnom analizom, sekvenciranjem delova genoma pozitivnih uzoraka stolice, izvrši genetska tipizacija i odredi filogenetska pripadnost virusa. Materijal i metode: Tokom petogodišnjeg istraživanja molekularnim real-time PCR testom pregledane su 1003 obolele osobe sa simptomima virusnog dijarealnog sindroma, starosti od mesec dana do preko 90 godina. Pregledani su na rota, noro, astro i enterične adenoviruse. Na osnovu podataka iz anketnih upitnika i istorija bolesti, detaljno su analizirani svi klinički pokazatelji (javljanje bolesti tokom godine, trajanje bolesti, simptomi). Procena težine kliničke slike vršena je prema Vesikari skali. Svi podaci su upoređivani prema vrsti virusnog uzroč, Introduction: Viral gastrointestinal syndrome is a current ongoing health problem worldwide. This is true of both developed and developing countries, especially underdeveloped ones where it is the second leading cause of mortality. Sudden onset of the disease—accompanied by the occurrence of large numbers of liquid stools, nausea, vomiting, abdominal pain, fever, and exhaustion—leads to dehydration. A fatal outcome can occur in all age groups of patients, especially very young children, the elderly, and the immuno-deficient, unless an accurate etiological diagnosis of the disease is quickly established, followed by a prompt institution of fluid and electrolyte placement, and implementation of other symptomatic therapy measures. Quick establishment of an accurate diagnosis, which is best achieved using the real-time PCR test, prevents the onset of complications, including a potentially fatal outcome of the disease. Simultaneously, it enables the implementation of appropriate epidemiological measures to prevent epidemic outbreaks and their spread. The aim of this study was to accurately determine the incidence of viral gastrointestinal syndrome in Vojvodina and the frequency of epidemic and sporadic occurrence of this disease. The aim was also to set up an algorithm for the application of the real-time PCR test in diagnostics of viral gastrointestinal syndrome in future work. Likewise, the aim was to carry out genetic typing and determine phylogenetic affiliation of the virus using molecular analysis and sequencing of parts of genomes from positive stool samples. Material and Methods: During a five-year study, 1003 patients with symptoms of viral diarrheal syndrome, aged from one month to more than 90 years old, were examined using molecular real-time PCR test. They were screened for rota, noro, astro, and enteric adenoviruses. Based on the data from survey questionnaires and medical case history, all clinical indicators were meticulously analyzed (disease occurrence
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- 2018
28. Diagnosis of west Nile neuroinvasive disease in humans
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Hrnjaković Cvjetković, Ivana, primary, Milošević, Vesna, additional, Petrović, Tamaš, additional, Petrić, Dušan, additional, Kovačević, Gordana, additional, Radovanov, Jelena, additional, Patić, Aleksandra, additional, Nikolić, Nataša, additional, Stefan Mikić, Sandra, additional, Cvjetković, Sofija, additional, and Cvjetković, Dejan, additional
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- 2018
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29. Interleukin 6 u maternalnom serumu kao marker bakterijske infekcije i prijevremenog porođaja
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Erić, Želimir, primary, Patić, Aleksandra, additional, Bogavac, Mirjana, additional, and Petrović Tepić, Snežana, additional
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- 2017
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30. DIAGNOSIS OF WEST NILE NEUROINVASIVE DISEASE IN HUMANS.
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Cvjetković, Ivana Hrnjaković, Milošević, Vesna, Petrović, Tamaš, Petrić, Dušan, Kovačević, Gordana, Radovanov, Jelena, Patić, Aleksandra, Nikolić, Nataša, Mikić, Sandra Stefan, Cvjetković, Sofija, and Cvjetković, Dejan
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WEST Nile virus ,MENINGOENCEPHALITIS ,ACUTE flaccid paralysis ,IMMUNOGLOBULINS ,REVERSE transcriptase polymerase chain reaction - Abstract
Copyright of Archives of Veterinary Medicine / Arhiv Veterinarske Medicine is the property of Scientific Veterinary Institute Novi Sad and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2018
31. USUTU VIRUS: AN EMERGING FLAVIVIRUS IN EUROPE.
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Cvjetković, Ivana Hrnjaković, Petrović, Tamaš, Petrić, Dušan, Milošević, Uroš, Radovanov, Jelena, Kovačević, Gordana, Galović, Aleksandra Jovanović, Patić, Aleksandra, Nikolić, Nataša, Cvjetković, Dejan, Mikić, Sandra Stefan, and Milošević, Vesna
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ARBOVIRUSES ,FLAVIVIRUSES ,VETERINARY medicine ,ENCEPHALITIS ,MYOCARDITIS - Abstract
Copyright of Archives of Veterinary Medicine / Arhiv Veterinarske Medicine is the property of Scientific Veterinary Institute Novi Sad and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2017
32. SEROPREVALENCE OF MOSQUITO-BORN AND TICK-BORN MICROORGANISMS IN HUMAN POPULATION OF SOUTH BACKA DISTRICT.
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Cvjetković, Ivana Hrnjaković, Petrović, Tamaš, Petrić, Dušan, Cvjetković, Dejan, Kovačević, Gordana, Radovanov, Jelena, Galović, Aleksandra Jovanović, Patić, Aleksandra, Nikolić, Nataša, Mikić, Sandra Stefan, and Milošević, Vesna
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SEROPREVALENCE ,TICK-borne diseases ,CHIKUNGUNYA virus ,AEDES aegypti ,BORRELIA - Abstract
Copyright of Archives of Veterinary Medicine / Arhiv Veterinarske Medicine is the property of Scientific Veterinary Institute Novi Sad and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2016
33. TICK-BORNE ENCEPHALITIS VIRUS INFECTION IN HUMANS.
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HRNJAKOVIĆ CVJETKOVIĆ, Ivana, CVJETKOVIĆ, Dejan, PATIĆ, Aleksandra, RADOVANOV, Jelena, KOVAČEVIĆ, Gordana, and MILOŠEVIĆ, Vesna
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CENTRAL nervous system diseases , *TICK-borne diseases , *MENINGOENCEPHALITIS , *FLAVIVIRUSES , *ETIOLOGY of diseases , *DISEASE vectors , *DIAGNOSIS , *INFECTIOUS disease transmission , *THERAPEUTICS - Abstract
Introduction. Tick-borne meningoencephalitis virus is a flavivirus that causes the most important vector-borne central nervous system infection in many countries of Europe and Asia. There are three subtypes of tick-borne encephalitis virus: European, Siberian and the Far-Eastern subtype. Transmission. In endemic areas, the virus remains in transmissive cycles between Ixodes ticks and small rodents. Clinical picture. In most cases (70− 98%) infection goes asymptomatically. In about one-third of meningitis cases, meningoencephalitis or meningomyelitis is developed. Postencephalytic syndrome may be the complication of the infection, presenting with neurological symptoms. Diagnosis. Etiologic diagnosis of tick-borne meningoencephalitis is only made on basis of laboratory analyses. Reverse transcription-polymerase chain reaction is used for determining the presence of virus in the blood and cerebrospinal fluid. Antibodies in blood and cerebrospinal fluid can be detected by serological tests. Prevention. The most efficient way to control this potentially severe disease with possible serious long-term consequences is vaccination. It should be recommended to persons who live or travel to endemic areas. Conclusion. In Serbia, tick-borne encephalitis virus infection belongs to the list of reportable diseases; however, there are no reported cases because the diagnostics is not performed routinely. We believe that the significance of this zoonosis must be examined in our country and some of its parts because of preliminary positive serological findings found out in Vojvodina as well as because of reported cases in neighboring countries such as Hungary and Croatia and its worldwide distribution. [ABSTRACT FROM AUTHOR]
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- 2016
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34. CHIKUNGUNYA -- A SERIOUS THREAT FOR PUBLIC HEALTH.
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HRNJAKOVIĆ CVJETKOVIĆ, Ivana B., CVJETKOVIĆ, Dejan, PATIĆ, Aleksandra, NIKOLIĆ, Nataša, STEFAN MIKIĆ, Sandra, and MILOŠEVIĆ, Vesna
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CHIKUNGUNYA , *PUBLIC health , *COMMUNICABLE diseases , *TOGAVIRUSES , *EPIDEMIOLOGY , *DISEASE risk factors - Abstract
Introduction. Chikungunya is a contagious disease caused by Chikungunya virus, an arbovirus from the Togaviridae family. This infection is mostly spread by mosquitoes from the genus Aedes, especially Aedes albopictus, which have spread from Asia to America and Europe including some countries surrounding Serbia. Epidemiologic Features. The outbreak of epidemics has been reported in Philippines, Sumatra, Java, Indonesia, West Africa region (from Senegal to Cameroon), Congo, Nigeria, Angola, Uganda, Guinea, Malawi, Central African Republic, Burundi, South Africa and India. At the beginning of the 21st century, large outbreaks were recorded on the island of Réunion. During 2006, 1.400.000 cases of chikungunya infection were recorded in India. Local transmission of infection in continental Europe was reported from Northeast Italy (254 suspected and 78 laboratory confirmed cases in Emilia-Romagna region) and France (two cases in 2010). From December 2013 to June 2014, 5.294 confirmed cases and more than 180.000 suspected cases of chikungunya were reported in the Caribbean. Clinical Findings. The disease presents suddenly with fever, rush and arthralgia. In general, chikungunya is a mild self - limited disease. Less often, it may be presented with signs of meningoencephalitis or fulminant hepatitis, sometimes with fatal outcome. Conclusion. Fast developing international traffic and booming tourism as well as the vector spreading from its homeland make chikungunya a real threat to our country. [ABSTRACT FROM AUTHOR]
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- 2015
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35. SIGNIFICANCE OF IgG AVIDITY TEST IN DIAGNOSIS OF WEST NILE VIRUS INFECTION.
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CVJETKOVIĆ, Ivana HRNJAKOVIĆ, RADOVANOV, Jelena, KOVAČEVIĆ, Gordana, PATIĆ, Aleksandra, NIKOLIĆ, Nataša, and MILOŠEVIĆ, Vesna
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IMMUNOGLOBULIN G , *WEST Nile fever , *SEROLOGY , *BLOOD sampling , *ENZYME-linked immunosorbent assay , *DIFFERENTIAL diagnosis - Abstract
Introduction. Serological tests appear to be the method of choice for establishing the diagnosis in the late phase of West Nile virus infection. Long persistence of IgM antibodies against West Nile virus is described and may be a problem for determination of the time of acquisition of West Nile virus infection. The aim of the study was to estimate the significance of IgG avidity determination in establishing the diagnosis of West Nile virus infection. Material and Methods. In a study 56 serum samples seropositive against West Nile virus were included. 24 serum samples were collected in 2012 from healthy residents of South-Backa district and 32 serum samples were collected in 2014 from 124 patients suspected of having West Nile virus infection. Commercial enzyme-linked immunosorbent tests were used for the detection of West Nile virusspecific IgM and IgG antibodies and IgG avidity. Results. Out of 124 patients suspected of having West Nile virus infection, 32 (25.8%) were seropositive for West Nile virus antibodies. Acute infection was laboratory confirmed in 15 (46.9%) cases. All patients with acute infection were West Nile virus IgM positive, 13 (85%) were West Nile virus IgG positive, and 2 (15%) had a borderline result for West Nile virus IgG antibodies. Out of 32 seropositive patients the presence of IgM antibodies was determined in 22 (68.7%). In a group of samples with high IgG avidity values, 6 were IgM positive, while 8 were IgM negative. Conclusion. West Nile virus IgM and IgG antibody serological assays alone are not sufficient for the accurate and reliable diagnosis of WNV infection. West Nile virus IgG avidity testing is necessary to ensure the differential diagnosis of acute from past West Nile virus infection. [ABSTRACT FROM AUTHOR]
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- 2017
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36. INFEKCIJE TOKSOPLAZMOM GONDII KOD GRAVIDNIH ŽENA.
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PATIĆ, Vera JERANT, MILOŠEVIĆ, Vesna, CVJETKOVIĆ, Ivana HRNJAKOVIĆ, PATIĆ, Aleksandra, MIKIĆ, Sandra STEFAN, and RISTIĆ, Marina
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TOXOPLASMA gondii , *PROTOZOAN diseases , *PREGNANCY complications , *TOXOPLASMOSIS , *SEROLOGY , *ENZYME-linked immunosorbent assay - Abstract
Introduction. The aim of this study was to determine the percentage of seropositive pregnant women, i.e. of pregnant women infected with Toxoplasma gondii in order to provide an insight into the risk of developing congenital toxoplasmosis in our community. Material and Methods. In the period of two years, 662 pregnant women from Vojvodina were examined serologically. The enzyme-linked immunosorbent assay tests were performed to determine IgM and IgG antibodies against Toxoplasma gondii and the complement fixation test was done to detect total antibodies against Toxoplasma gondii. Results and Discussion. Seropositivity was determined in 180 pregnant women (27.19%). Of 135 pregnant women examined in the routine control in pregnancy, 16.30% were seropositive and out of five proven, completely new Toxoplasma gondii infections, three were detected in pregnant women who had undergone the routine check-up for no specific symptoms. A detailed analysis of the frequency of seropositive findings in relation to clinical diagnoses and the place of residence of pregnant women (urban and rural areas) was performed. At the same time, the results from the serological reactions were presented, commented and interpreted, and recommendations were given for the implementation of additional examinations (eg, IgG antibody avidity test) in order to make the accurate diagnosis. Conclusion. It can be concluded that the occurrence of congenital toxoplasmosis is still a problem in our community and that the best prevention is the prompt and adequate examination of pregnant women for the presence of Toxoplasma gondii infection. [ABSTRACT FROM AUTHOR]
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- 2013
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37. [Toxoplasma gondii infection in pregnant women].
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Jerant PV, Milosević V, Hrnjaković Cvjetković I, Patić A, Stefan Mikić S, and Ristić M
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- Adult, Antibodies, Protozoan blood, Complement Fixation Tests, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Pregnancy, Pregnancy Complications, Infectious diagnosis, Pregnancy Complications, Infectious immunology, Pregnancy Outcome, Seroepidemiologic Studies, Toxoplasma immunology, Toxoplasmosis diagnosis, Toxoplasmosis immunology, Pregnancy Complications, Infectious epidemiology, Toxoplasma isolation & purification, Toxoplasmosis epidemiology
- Abstract
Introduction: The aim of this study was to determine the percentage of seropositive pregnant women, i.e. of pregnant women infected with Toxoplasma gondii in order to provide an insight into the risk of developing congenital toxoplasmosis in our community., Material and Methods: In the period of two years, 662 pregnant women from Vojvodina were examined serologically. The enzyme-linked immunosorbent assay tests were performed to determine IgM and IgG antibodies against Toxoplasma gondii and the complement fixation test was done to detect total antibodies against Toxoplasma gondii., Results and Discussion: Seropositivity was determined in 180 pregnant women (27.19%). Of 135 pregnant women examined in the routine control in pregnancy, 16.30% were seropositive and out of five proven, completely new Toxoplasma gondii intfections, three were detected in pregnant women who had undergone the routine check-up for no specific symptoms. A detailed analysis of the frequency of seropositive findings in relation to clinical diagnoses and the place of residence of pregnant women (urban and rural areas) was performed. At the same time, the results from the serological reactions were presented, commented and interpreted, and recommendations were given for the implementation of additional examinations (eg, IgG antibody avidity test) in order to make the accurate diagnosis., Conclusion: It can be concluded that the occurrence of congenital toxoplasmosis is still a problem in our community and that the best prevention is the prompt and adequate examination of pregnant women for the presence of Toxoplasma gondii infection.
- Published
- 2013
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