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Your search keyword '"Patankar, Himanshu"' showing total 14 results
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14 results on '"Patankar, Himanshu"'

6. 2 Confers Drought and Salinity Tolerance to Yeast and Arabidopsis

Author

Patankar, Himanshu V., Al-Harrasi, Ibtisam, Al-Yahyai, Rashid, and Yaish, Mahmoud W.

Subjects
body regions, congenital, hereditary, and neonatal diseases and abnormalities, abiotic stress, functional characterization, food and beverages, aquaporins, salinity, date palm
Abstract

Recent studies on salinity tolerance in date palm revealed the discovery of salt-responsive genes including PdPIP1, 2, a highly conserved aquaporin gene in plants, which was functionally characterized in this study to investigate its precise role in drought and salinity tolerance. Immunoblot assay showed a high level of PIP1 protein accumulation only in the leaves of date palm plants when grown under drought, an observation which may imply the involvement of PIP1, 2 in CO2 uptake. Heterologous overexpression of PdPIP1, 2 in yeast (Saccharomyces cerevisiae) improved tolerance to salinity and oxidative stress. While, heterologous overexpression of PdPIP1, 2 in Arabidopsis had significantly (p &lt, 0.05) increased biomass, chlorophyll content, and root length under drought and salinity. In addition, a significantly (p &lt, 0.05) higher percentage of transgenic plants could be recovered by rewatering after drought stress, indicating the ability of the transgenic plants to maintain water and viability under drought. Transgenic plants under drought and salinity maintained significantly (p &lt, 0.05) higher K+/Na+ ratios than wild type (WT) plants, an observation which may represent an efficient tolerance mechanism controlled by the transgene. Collectively, this study provided an insight on the mechanism by which PdPIP1, 2 conferred tolerance to salt and drought stresses in date palm.

Published
2019
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14. Identification of Candidate Genes Involved in the Salt Tolerance of Date Palm (<italic>Phoenix dactylifera</italic> L.) Based on a Yeast Functional Bioassay.

Author

Patankar, Himanshu V., Al-Harrasi, Ibtisam, Al-Yahyai, Rashid, and Yaish, Mahmoud W.

Subjects
*DATE palm, *ANTISENSE DNA, *GENE expression, *POLYMERASE chain reaction, *AQUAPORINS, *SACCHAROMYCES cerevisiae
Abstract

Although date palm is a relatively salt-tolerant plant, the molecular basis of this tolerance is complex and poorly understood. Therefore, this study aimed to identify the genes involved in salinity tolerance using a basic yeast functional bioassay. To achieve this, a date palm cDNA library was overexpressed in Saccharomyces cerevisiae cells. The expression levels of selected genes that make yeast cells tolerant to salt were subsequently validated in the leaf and root tissues of date palm seedlings using a quantitative PCR method. About 6000 yeast transformant cells were replica printed and screened on a synthetic minimal medium containing 1.0 M of NaCl. The screening results showed the presence of 62 salt-tolerant transformant colonies. Sequence analysis of the recombinant yeast plasmids revealed the presence of a group of genes with potential salt-tolerance functions, such as aquaporins (PIP), serine/threonine protein kinases (STKs), ethylene-responsive transcription factor 1 (ERF1), and peroxidases (PRX). The expression pattern of the selected genes endorsed the hypothesis that these genes may be involved in salinity tolerance, as they showed a significant (p < 0.05) overexpression trend in both the leaf and root tissues in response to salinity. The genes identified in this project are suitable candidates for the further functional characterization of date palms. [ABSTRACT FROM AUTHOR]

Published
2018
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