9 results on '"Passos, Vania"'
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2. Susceptibility of Chikungunya Virus to Inactivation by Heat and Commercially and World Health Organization-Recommended Biocides
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Franz, Sergej, Friesland, Martina, Passos, Vânia, Todt, Daniel, Simmons, Graham, Goffinet, Christine, and Steinmann, Eike
- Published
- 2018
3. Innate immune response to SARS-CoV-2 infection contributes to neuronal damage in human iPSC-derived peripheral neurons
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Passos, Vania, primary, Henkel, Lisa M., additional, Wang, Jiayi, additional, Zapatero-Belinchón, Francisco J., additional, Möller, Rebecca, additional, Sun, Guorong, additional, Waltl, Inken, additional, Ritter, Birgit, additional, Kropp, Kai A., additional, Zhu, Shuyong, additional, Deleidi, Michela, additional, Kalinke, Ulrich, additional, Höglinger, Günter, additional, Gerold, Gisa, additional, Wegner, Florian, additional, and Viejo-Borbolla, Abel, additional
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- 2022
- Full Text
- View/download PDF
4. Interfaces do Pibid/UFT: a gestão na formação inicial docente
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Padovan, Regina Célia, primary, Silva, Raielly Soares da, additional, and Passos, Vania Maria de Araujo, additional
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- 2020
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5. PIBID e Formação Docente: políticas públicas e práticas educativas
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Padovan, Regina Célia, primary, Pereira, Fabíola Andrade, additional, and Passos, Vania Maria de Araujo, additional
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- 2020
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6. Quantitative Proteomics of Uukuniemi Virus-host Cell Interactions Reveals GBF1 as Proviral Host Factor for Phleboviruses
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Uckeley, Zina M., primary, Moeller, Rebecca, additional, Kühn, Lars I., additional, Nilsson, Emma, additional, Robens, Claudia, additional, Lasswitz, Lisa, additional, Lindqvist, Richard, additional, Lenman, Annasara, additional, Passos, Vania, additional, Voss, Yannik, additional, Sommerauer, Christian, additional, Kampmann, Martin, additional, Goffinet, Christine, additional, Meissner, Felix, additional, Överby, Anna K., additional, Lozach, Pierre-Yves, additional, and Gerold, Gisa, additional
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- 2019
- Full Text
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7. Quantitative Proteomics of Uukuniemi Virus-host Cell Interactions Reveals GBF1 as Proviral Host Factor for Phleboviruses
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Uckeley, Zina M., Moeller, Rebecca, Kuhn, Lars I., Nilsson, Emma, Robens, Claudia, Lasswitz, Lisa, Lindquist, Richard, Lenman, Annasara, Passos, Vania, Voss, Yannik, Sommerauer, Christian, Kampmann, Martin, Goffinet, Christine, Meissner, Felix, Överby, Anna K., Lozach, Pierre-Yves, Gerold, Gisa, Uckeley, Zina M., Moeller, Rebecca, Kuhn, Lars I., Nilsson, Emma, Robens, Claudia, Lasswitz, Lisa, Lindquist, Richard, Lenman, Annasara, Passos, Vania, Voss, Yannik, Sommerauer, Christian, Kampmann, Martin, Goffinet, Christine, Meissner, Felix, Överby, Anna K., Lozach, Pierre-Yves, and Gerold, Gisa
- Abstract
Novel tick-borne phleboviruses in the Phenuiviridae family, which are highly pathogenic in humans and all closely related to Uukuniemi virus (UUKV), have recently emerged on different continents. How phleboviruses assemble, bud, and exit cells remains largely elusive. Here, we performed high-resolution, label-free mass spectrometry analysis of UUKV immunoprecipitated from cell lysates and identified 39 cellular partners interacting with the viral envelope glycoproteins. The importance of these host factors for UUKV infection was validated by silencing each host factor by RNA interference. This revealed Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1), a guanine nucleotide exchange factor resident in the Golgi, as a critical host factor required for the UUKV life cycle. An inhibitor of GBF1, Golgicide A, confirmed the role of the cellular factor in UUKV infection. We could pinpoint the GBF1 requirement to UUKV replication and particle assembly. When the investigation was extended to viruses from various positive and negative RNA viral families, we found that not only phleboviruses rely on GBF1 for infection, but also Flavi-, Corona-, Rhabdo-, and Togaviridae. In contrast, silencing or blocking GBF1 did not abrogate infection by the human adenovirus serotype 5 and immunodeficiency retrovirus type 1, the replication of both requires nuclear steps. Together our results indicate that UUKV relies on GBF1 for viral replication, assembly and egress. This study also highlights the proviral activity of GBF1 in the infection by a broad range of important zoonotic RNA viruses. Ticks are important vectors of infectious emerging diseases and tick-borne phleboviruses represent a growing threat to humans globally. We employed here a high-resolution, label-free mass spectrometry and RNA interference screen approach to reveal the host cell protein GBF1 as a proviral factor, not only for tick-borne phleboviruses, but also for many other important zoono
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- 2019
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8. Characterization of Endogenous SERINC5 Protein as Anti-HIV-1 Factor
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Passos, Vania, Zillinger, Thomas, Casartelli, Nicoletta, Wachs, Amelie S., Xu, Shuting, Malassa, Angelina, Steppich, Katja, Schilling, Hildegard, Franz, Sergej, Todt, Daniel, Steinmann, Eike, Sutter, Kathrin, Dittmer, Ulf, Bohne, Jens, Schwartz, Olivier, Barchet, Winfried, Goffinet, Christine, Passos, Vania, Zillinger, Thomas, Casartelli, Nicoletta, Wachs, Amelie S., Xu, Shuting, Malassa, Angelina, Steppich, Katja, Schilling, Hildegard, Franz, Sergej, Todt, Daniel, Steinmann, Eike, Sutter, Kathrin, Dittmer, Ulf, Bohne, Jens, Schwartz, Olivier, Barchet, Winfried, and Goffinet, Christine
- Abstract
When expressed in virus-producing cells, the cellular multipass transmembrane protein SERINC5 reduces the infectivity of HIV-1 particles and is counteracted by HIV-1 Nef. Due to the unavailability of an antibody of sufficient specificity and sensitivity, investigation of SERINC5 protein expression and subcellular localization has been limited to heterologously expressed SERINC5. We generated, via CRISPR/Cas9-assisted gene editing, Jurkat T-cell clones expressing endogenous SERINC5 bearing an extracellularly exposed hemagglutinin (HA) epitope [Jurkat SERINC5(iHA knock-in) T cells]. This modification enabled quantification of endogenous SERINC5 protein levels and demonstrated a predominant localization in lipid rafts. Interferon alpha (IFN-alpha) treatment enhanced cell surface levels of SERINC5 in a ruxolitinib-sensitive manner in the absence of modulation of mRNA and protein quantities. Parental and SERINC5(iHA knock-in) T cells shared the ability to produce infectious wild-type HIV-1 but not an HIV-1 Delta nef mutant. SERINC5-imposed reduction of infectivity involved a modest reduction of virus fusogenicity. An association of endogenous SERINC5 protein with HIV-1 Delta nef virions was consistently detectable as a 35-kDa species, as opposed to heterologous SERINC5, which presented as a 51-kDa species. Nef-mediated functional counteraction did not correlate with virion exclusion of SERINC5, arguing for the existence of additional counteractive mechanisms of Nef that act on virusassociated SERINC5. In HIV-1-infected cells, Nef triggered the internalization of SERINC5 in the absence of detectable changes of steady-state protein levels. These findings establish new properties of endogenous SERINC5 expression and subcellular localization, challenge existing concepts of HIV-1 Nef-mediated antagonism of SERINC5, and uncover an unprecedented role of IFN-alpha in modulating SERINC5 through accumulation at the cell surface. IMPORTANCE SERINC5 is the long-searched-for antivira
- Published
- 2019
9. The job of teachers and the Course of Pedagoy in the Federal University of Tocantins
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PASSOS, Vania Maria de Araujo and SOUZA, Ruth Catarina Cerqueira Ribeiro de
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University ,Profissão Docente ,Curso de Pedagogia ,Course of Pedagogy ,Teachers job ,CIENCIAS HUMANAS::EDUCACAO [CNPQ] ,Universidade - Abstract
Integrante da Linha de pesquisa: Formação, Profissionalização Docente e Práticas Educativas, o presente trabalho apresenta a discussão da profissão docente, em Cursos de Pedagogia, como um dos elementos que envolvem a complexidade da profissionalização. Entretanto, é uma discussão que nos remete à relação de significados entre o profissional em formação (sujeito) e a profissão docente (objeto), levando-nos a refletir sobre o pensar e o agir, tanto de forma individual como coletiva, para compreender o processo educativo e formativo da docência na universidade. Neste sentido, apresentamos por objetivo geral: compreender como e sob quais aspectos o conhecimento da profissão docente nos Cursos de Pedagogia da Universidade Federal do Tocantins (UFT), tem sido desenvolvido. Adotamos o caminho para construir o diálogo entre conhecimentos, dados e análise da profissão docente a partir da perspectiva materialista histórica dialética, como método de investigação e de análise e valemo-nos do Estudo de Caso, para aproximação do objeto de estudo. A coleta de dados foi fundamentada em revisão bibliográfica, análise documental e aplicação de questionário aos docentes formadores e discentes dos cursos de Pedagogia. Conforme os dados analisados, apesar da coexistência de projetos distintos em desenvolvimento na UFT, os posicionamentos dos discentes e docentes, referentes à uma concepção da profissão, não resultaram da adesão a um Projeto Pedagógico de Curso específico, mas foram expressos em função das construções pessoais e individuais e da experiência dos docentes, na educação superior, acerca da profissão docente. Neste sentido, a relação entre o sujeito e o objeto e a compreensão da profissão docente não são estabelecidas em relações rígidas, mas estão presentes na ambiguidade, na contradição e na constante reelaboração do conhecimento, ainda que não se alcance o que foi proposto pelo Projeto Pedagógico do Curso. A dinâmica da realidade educativa não é compatível com o conformismo ou naturalização de fatos e de teorias, e nos incita a pensar o movimento do pensamento e da reflexão acerca das necessidades e interesses de enfrentar o fenômeno educativo vivenciado na instituição educativa, como cultura geral e profissional para o docente. Para além do conteúdo identificado nos discursos e práticas realizadas nos Cursos de Pedagogia da UFT, a contribuição deste trabalho se dá no sentido de confirmar a coexistência das ambigüidades e incertezas frente à profissão docente, no interior da formação inicial. Taking part of the research line Formation and Professionalization of teachers and Educational Practices, this study presents the discussion about the job of teacher in courses of Pedagogy as one of the elements which involve the complexity of professionalization. It is, however, a discussion that send us to the relationship of meanings of professionals in situation of formation (subject) and the job (object), leading us to reflect about thinking and acting, both in an individual and collective form in order to understand the educational and forming process of teachers job in the university. So, we presented as our general goal: understand how and under which aspects has been developed the knowledge of teachers job in the courses of Pedagogy of the Federal University of Tocantins (UFT). We adopted the way of building the dialogue between knowledges, data and analyses of teachers job, under materialistic historic and dialectic view, as a method of investigation and analysis. In order to do it, however, we used the study of case as the form of pursuing the object of study. The collection of data was based in a bibliographic revision, in a documental analysis and in an application of questionnaires to professors and students of the courses of Pedagogy. According to the analyzed data, despite the coexistence of different projects in development in UFT, the postures of professors and students, referring to a conception of the job did not result from the adherence to an specific Pedagogic Project of Course, but they have been expressed in dependence of personal and individual constructions and of the experience of professors in higher education about teachers job. So, the relation between subject and object and the comprehension of teachers job were not established in rigid relationships, but they are present in the ambiguity, in the contradiction and in the constant remaking of knowledge, although they don t reach whatever has been proposed by the Pedagogic Project of the course. The dynamics of the educational reality is not compatible to conformism or naturalization of facts and theories an lead us to think about the movement and of the reflection about interests and needs of facing the educational phenomena lived in an educational institution, as general and professional culture professors. Beyond contents identified in speeches and practices carried out in the courses of Pedagogy, the contribution of this work is to confirm the coexistence of ambiguities and uncertainties in teachers job, inside their early formation.
- Published
- 2011
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