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Copyright of Giornale Italiano di Medicina del Lavoro ed Ergonomia is the property of Giornale Italiano di Medicina del Lavoro ed Ergonomia Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Española de Cirugía Oral y Maxilofacial is the property of Sociedad Espanola de Cirugia Oral y Maxilofacial (SECOM) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Activating mutations of the BRAF gene are the most common genetic alterations in papillary thyroid carcinomas (PTCs) and the T1799A transversion, resulting in BRAFV600E, appeared virtually unique in this cancer type. Here, we report on the identification in a classic PTC of a novel BRAF mutation, namely a 1795GTT insertion, resulting in BRAFV599Ins, and describe its biochemical and molecular characterization. Kinase assays carried out on BRAFV599Ins and BRAFV600E revealed a three- to five-fold increase in the enzymatic activity of both mutants with respect to BRAFWT. Similarly, evaluation of BRAF-induced phosphorylation of MEK, MAPK and RSK revealed a significant MAPK cascade activation in cells expressing BRAFV599Ins or BRAFV600E, but not in cells expressing BRAFWT. Molecular dynamic simulations showed a destabilization of the inactive conformation of the enzyme in both BRAFV599Ins and BRAFV600E mutants, but not in BRAFWT. The analysis of the interaction energies inside the catalytic site allowed to demonstrate the presence of repulsive electrostatic forces acting on the activation loop and moving from inward to outward of the mutant enzymes. Finally, focus assays in NIH-3T3 cells confirmed a high transformation rate in the cells transfected either with BRAFV599Ins or BRAFV600E. In conclusion, this study demonstrated that BRAFV599Ins, as BRAFV600E, is a ‘gain of function’ mutation, characterized by a constitutive catalytic activation, which accounts for its causative role in the studied PTC.Oncogene (2006) 25, 4235–4240. doi:10.1038/sj.onc.1209448; published online 27 February 2006 [ABSTRACT FROM AUTHOR]

Objectives: Radiographic assessment of marginal bone height is included in longitudinal control of osseointegrated implants. However, accurate and reproducible results are difficult to obtain. The aim of the present study was to examine the differences between linear measurements in digitized radiographs (DR) and digital subtraction images (DSI) around endosseous implants and the interobserver variability. Methods: The bone height around 30 implants in 22 patients was assessed by 5 observers. Standardized periapical radiographs were obtained just after the surgery and 4 months later. The radiographs were digitized and manipulated by means of EMAGO® software, and linear and logarithmic DSIs were produced. Furthermore, the logarithmic subtraction was enhanced with the use of a filter. The observers had the DRs and three methods of subtraction to assess bone height. ANOVA statistical procedures were applied to analyse differences between the observers in the four assessed images and the Tukey test was used to evaluate the differences between the images. Results: Comparison of the bone height assessments indicated significantly (P < 0.05) higher values in the DR than the three methods of DSI. The observers also had a statistically significant variability in this assessment (P = 0.00003). Conclusions: DSI demonstrated lower values of linear measurements of the bone height around endosseous implants, compared with DR. Interobserver variability should be considered when comparing values from follow-up studies. [ABSTRACT FROM AUTHOR]