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The authors analysed the various enzymes in the lymph of the thoracic duct and the right lymphatic duct in a series of 15 dogs submitted to extracorporeal circulation, the experimentors noted a series of diffuse cellular lesions which involved transiently the main organs. This lesion seems only to occur at first: independent of the duration of the by-pass, probably due to hypoxia following the rapid and massive introduction of priming. It is concluded that lymph analysis is more revealing for knowledge of enzyme behaviour than blood analysis. A differential study of lymph in the right lymphatic duct and in the thoracic duct is surprisingly much less important.

TBW can be performed even without help of a cardiopulmonary machine (CPM). Low temperature and Ht levels are compatible with cardiac, and cerebral electric activity. Such conditions may be reached only by perfusing cold liquids and utilizing the cardiac and respiratory activity of the animals, with no use of machines.

Three different techniques of total body washout (T.B.W.) have been used in experiments dealing with rats. The first technique was employing inferior vena cava as input and abdominal aorta as output; the second one performed just the opposite way; finally the third used abdominal aorta at the start of the experiment, whereas inferior vena cava was selected at the end. The last one proved to be the most efficient technique in comparison to the other ones.