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This study was conducted to test the effects of supplemental L-Met (CJ Corp, Seoul, Korea) on redox status, gut development, and growth performance of turkey poults compared with supplemental DL-Met. Three hundred eighty five newly hatched turkey poults were weighed and randomly allotted to 5 treatments in a completely randomized design for 28 d, including a basal diet (BD), the BD +0.145% DL-Met or L-Met, the BD + 0.285% DL-Met or L-Met (representing 60, 75, and 90% of NRC digestible Met + Cys requirement). Feed disappearance and BW were measured weekly. Liver, duodenum, and jejunum samples were collected from 2 random selected birds in each cage on d 7 and 28. Hepatic tissue and duodenal mucosa were used to measure glutathione (GSH), total antioxidant capacity (TAC), protein carbonyl (PC), and malondialde-hyde (MDA) as indicators of the redox status. Duodenal and jejunal tissues were used to evaluate morphology. Data were analyzed using the Mixed procedure in SAS. A multilinear regression analysis was used to evaluate the relative bioavailability (RBA) of L-Met to DL-Met. Overall, weight gain (690 to 746 g) and feed intake (1123 to 1248 g) were increased (P < 0.05) as increasing digestible Met + Cys levels from 60 to 90% of the NRC requirement. Birds with L-Met tended (P = 0.053) to have decreased F:G (1.70 to 1.63) compared with birds with DL-Met. Rates of decrease in F:G were different (P < 0.05) between birds with DL-Met and L-Met. Supplementing Met regardless of sources decreased (P < 0.05) MDA (3.29 to 2.47 μmol/g protein) in duodenal mucosa compared with birds in the BD. Supplementing L-Met tended (P < 0.094) to decrease MDA (1.27 to 1.16 Limol/g protein) in hepatic tissues compared with the supplementation of DL-Met. Other oxidative status markers including GSH, TAC, and PC were not affected by Met sources. Supplementation of Met either from DL or L forms did not affect morphology of duodenum and jejunum on d 7 and 28. In conclusion, supplementation of Met enhanced the growth performance and reduced oxidative stress in the gut of turkey poults during the first 28 d of age. Use of L-Met can potentially enhance feed efficiency and reduce systemic oxidative stress compared with the use of DL-Met in turkeys. [ABSTRACT FROM AUTHOR]

Objective: The objective of this study was to determine the effects of β-mannanase on metabolizable energy (ME) and apparent total tract digestibility (ATTD) of protein in various feedstuffs including barley, copra meal, corn, corn distillers dried grains with solubles (DDGS), palm kernel meal, sorghum, and soybean meal., Methods: A basal diet was formulated with 94.8% corn and 0.77% amino acids, minerals, and vitamins and test diets replacing corn-basal diets with barley, corn DDGS, sorghum, soybean meal, or wheat (50%, respectively) and copra meal or palm kernel meal (30%, respectively). The basal diet and test diets were evaluated by using triplicated or quadruplicated 2×2 Latin square designs consisting of 2 diets and 2 periods with a total of 54 barrows at 20.6±0.6 kg (9 wk of age). Dietary treatments were levels of β-mannanase supplementation (0 or 800 U/kg of feed). Fecal and urine samples were collected for 4 d following a 4-d adaptation period. The ME and ATTD of crude protein (CP) in feedstuffs were calculated by a difference procedure. Data were analyzed using Proc general linear model of SAS., Results: Supplementation of β-mannanase improved (p<0.05) ME of barley (10.4%), palm kernel meal (12.4%), sorghum (6.0%), and soybean meal (2.9%) fed to growing pigs. Supplementation of β-mannanase increased (p<0.05) ATTD of CP in palm kernel meal (8.8%) and tended to increase (p = 0.061) ATTD of CP in copra meal (18.0%) fed to growing pigs., Conclusion: This study indicates that various factors such as the structure and the amount of β-mannans, water binding capacity, and the level of resistant starch vary among feedstuffs and the efficacy of supplemental β-mannanase may be influenced by these factors.