Search

Fil: Pasqualetti, Mariana I. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias; Argentina Fil: Pasqualetti, Mariana I. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Pasqualetti, Mariana I. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fariña, Fernando A. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias; Argentina Fil: Fariña, Fernando A. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Fariña, Fernando A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Krivokapich, Silvio J. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina Fil: Gatti, Genoveva M. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina Fil: Daneri, Gustavo A. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de CienciasNaturales,“Bernardino Rivadavia”; Argentina Fil: Varela, Esperanza A. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de CienciasNaturales,“Bernardino Rivadavia”; Argentina Fil: Lucero, Sergio. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de CienciasNaturales,“Bernardino Rivadavia”; Argentina Fil: Ercole, Mariano E. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias,Cátedra de Parasitología y Enfermedades Parasitarias; Argentina Fil: Bessi, Clara. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias,Cátedra de Parasitología y Enfermedades Parasitarias; Argentina Fil: Bessi, Clara. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Bessi, Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Winter, Marina. Universidad Nacional de Río Negro. Sede Atlántica; Argentina Fil: Winter, Marina. Centro de Investigación y Transferencia Río Negro; Argentina Fil: Ribicich, Mabel M. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Ribicich, Mabel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina Fil: Ribicich, Mabel M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina In Argentina trichinellosis is an endemic disease representing an important risk for human health due to its high rates of morbidity, mainly transmitted by the consumption of raw or undercooked pork. Nevertheless, the discovery of new Trichinella species have led to a change in the study of the epidemiology of the disease with the addition of new sources of infection. Moreover, Trichinella infection has been detected in a wide range of marine mammals around the world. Until the present time, Trichinella spp. infection has not been detected in marine mammals of South America. Four South American sea lions were found dead in the rookeries of Caleta de los Loros (Lat. 41° 00′ S; 64° 12′ W; n = 1), Promontorio Belén (Lat. 41° 09′ S; Long. 63° 48′ O; n = 1) and Punta Bermeja (Lat. 41° 09′ S; Long. 63° 09′ O; n = 2) in Rio Negro, Argentina. Muscle samples were taken from the tongue and diaphragm and were stored at 4 °C until examination at the Parasitology Laboratory of the Facultad de Ciencias Veterinarias, Universidad de Buenos Aires. The total muscle samples from each animal were analyzed by artificial digestión. Identification at the species level was made by nested multiplex chain reaction (nested multiplex PCR) based on nuclear ribosomal DNA sequences, using six pair of primers. Trichinella spp. larvae were found in one of the four South American sea lions. Based on their morphology, the recovered larvae were suggestive of Trichinella spp. Trichinella larvae generated a fragment of 173 bp corresponding to T. spiralis expansion segment V (ESV) region of the ribosomal DNA. This is the first report of a Trichinella species infecting marine mammals from South America. The inclusion of Otaria flavescens in the wide range of Trichinella hosts adds new questions to the epidemiology of Trichinella in marine animals. We thank the Secretaría de Medio Ambiente y Desarrollo Sustentable (SAyDS) of Río Negro Province for giving us permission to get sample from the rookeries. This work was supported by Universidad de Buenos Aires, Secretaria de Ciencia y Técnica Subsidio UBACyT20020130100336BA and Ministerio de Ciencia y Tecnología, FONCyT Subsidio PICT-2015-2350

Fil: Winter, Marina. Universidad Nacional de Río Negro. Sede Atlántica; Argentina Fil: Winter, Marina. Centro de Investigaciones y Transferencia Río Negro (CONICET-UNRN); Argentina Fil: Abate, Sergio D. Centro de Investigaciones y Transferencia Río Negro (CONICET-UNRN); Argentina Fil: Abate, Sergio D. Universidad Nacional de Río Negro. Sede Atlántica; Argentina Fil: Pasqualetti, Mariana I. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pasqualetti, Mariana I. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina Fil: Pasqualetti, Mariana I. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Fariña, Fernando A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fariña, Fernando A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina Fil: Fariña, Fernando A. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Ribicich, Mabel M. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Ribicich, Mabel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina Fil: Ribicich, Mabel M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Trichinellosis is endemic in Argentina and an important public health problem because of its high morbidity rates. In Patagonia Argentina, despite not being an important pig production area, there are outbreaks of trichinellosis due to the consumption without bromatological control of pigs meat produced domestically and wild animals meat obtained by hunting activity. Also, the production of sausages (“chacinados’’) for family consumption and informal sale contributes to the transmission. Even though one way to prevent trichinellosis is to cook meat to safe temperatures, the consumption of raw sausages should only be made after the artificial digestion method was performed on animal carcasses and a negative result was given. In this context, the aim of this study was to make workshops for children between 6 and 12 years old, tending to provide tools that reduce the appearance of outbreaks of trichinellosis in a rural area of the northern Patagonia Argentina. Under the concept of "know to prevent", the different aspects of the parasite-host-environment triad were worked on. Between August 2016 and December 2017, seven groups of students between 6 and 12 years old of two primary rural schools took part in workshops. To stimulate learning, parasites were observed through a microscope and magnifying glass. They were developed theoretical content and practical, creative and playful activities. In addition, attractive and easy-to-understand brochures were designed for children and families. As a result, 176 children from two rural primary schools in a rural area of the northern Patagonia Argentina learned for the first time what trichinellosis is, how it is transmitted and how it is prevented. To promote a necessary change of attitude tending to prevent trichinellosis, the active participation of children is essential.