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The metabolism of a continuous Ae. albopictus cell line (clone C6/36) was studied relative to the consumption of an environmental component by mammalian cells of the continuous lines Vero(B) and BNK-21 under steady-state cultivation conditions. The Ae. albopictus cells were found to utilize the amino acid glycine and on glycolysis they elaborated not more than (10...20)% of acid products (lactic acid) neutralized by sodium biocarbonate, suggesting that there were also other ways of using glucose. These features lead to the conclusion that the mosquito cells form an extracellular matrix. The findings were used to certify the culture of cells, to substantiate the composition of a cultivation medium, and to optimize the latter.

The essence of studies was that the disease is simulated in 12-day albino mice subcutaneously infected with Hantaan virus, strain 76-118 in a dose of 10 LD50. As an efficiency index, the study of drugs uses major (death protection coefficient, mean animal lifetime) and auxiliary (virological: pathogen accumulation in the brain tissues of deceased animals) parameters, biochemical (the levels of creatinine, urea, alanine aminotransferase, aspartate aminotransferase, malonic dialdehyde), hematological (count of leukocytes, leukogram) ones; as well as interferon status (the levels of circulatory interferon, leukocytic production of alpha- and gamma-interferons). The procedure for simulating the disease caused by Hantaan virus on an experimental animal is used to choose effective drugs against the pathogen of hemorrhagic nephrosonephritis.

It is shown that primary screening of drugs against the pathogens of hemorrhagic fever with renal syndrome (HFV) may be performed, by using two approaches in estimating the suppression of plague formation and that of viral reproduction in the cultured cells. It is expedient to make a primary screening of interferon and its inductors to estimate the suppression of viral reproduction in the cultured Vero E6, PSEV, and CL-17 cells, the infection multiplicity should be hundredth parts of BOE/cell. Among the test agents, there are virazole and realdiron that are the most active drugs against Hantaan virus, 76-118 strain, which virually completely suppress the reproduction of the study causative agent, when used even at concentrations of 1-5 microg/ml and 100 U/ml, respectively.

The antiviral effectiveness of the combined and single use of superlow-dose amixine and virasole on the course of experimental hemorrhagic fever with renal syndrome was studied in sucking albino mice parenterally infected with their virus Hantaan. The co-administration of virasole and amixine was shown to protect 52% of the infected animals from death, which is superior to the effect of their monotherapy. The combined use of the drugs substantially prolongs the survival of albino mice after their infection and the level of brain viral reproduction suppression ( delta = 3.21 g) in the experimental group as compared to the controls and to the mice given only one of the drugs.

Experimental research was undertaken to investigate the use of amixin in prevention, emergency prevention schemes and treatment of mice infected with West Nile fever (WNF) agent, strain Eg-101; the results are indicative of the drug efficiency both in its peroral and subcutaneous administrations. Amixin was shown to be most effective in the former case when administered, 10 mg/kg, in 96 hours before mice were infected as well as during the entire incubation period: lethality protection--46%. In the latter case, the drug was effective, when 3 administration schemes were in use, 10 mg/kg. The maximum degree of protection efficiency was registered with amixin administration according to the emergency prevention scheme: lethality protection--33%. The drug suppresses effectively the WNF virus reproduction in cerebral tissues.

The most promising trends of using monoclonal antibodies (Mabs) in virology research of the viral hemorrhagic-fevers' agents are related with studying viral antigen structure and with developing diagnostic preparations for indicating and identifying infectious agents of the mentioned pathologies. The methodological specificity of obtaining the Mabs to viral hemorrhagic-fevers' agents as well as data on its practical use are discussed.

Efficacy of drug therapy of Omsk hemorrhagic fever (OHF) was tested experimentally using cell culture and laboratory animals infected with the OHF virus strain Ondatra. Screening tests showed that high concentrations of Virazol or interferon inducers Larifan and Rifastin caused moderately pronounced suppression of virus reproduction in cell culture. Realdiron was found to be a high-efficacy preparation causing complete inhibition of virus reproduction in cell. Larifan demonstrated the highest antiviral efficacy against OHF virus in experiments with laboratory animals. This drug prevented the death of 65% infected mice and significantly decreased infection process severity in rabbits.

Hybridomas producing monoclonal antibodies (MAb) to Marburg virus glycoprotein were prepared by splicing of mouse myeloma cells (NSO and X.63-Ag6.653 strains) and splenocytes of immunized BALB/c mice. Cultural and MAb-producing properties of hybridomas were studied. The production of MAb during serial passages 15-30 was confirmed. MAb titers in culture fluids were 1:4096-1:8152, in immune ascitic fluids of BALB/c mice 1:409,600-1:638,400. The possibility of using MAbs as a component of ELISA test system was demonstrated. The sensitivity of ELISA-MAb method for Marburg virus was 1 x 10(3) PFU/mu.