1,003 results on '"Pascale, S."'
Search Results
2. Multiphase Kinetic Multilayer Model Interfaces for Simulating Surface and Bulk Chemistry for Environmental and Atmospheric Chemistry Teaching
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Hua, Amy K., Lakey, Pascale S. J., and Shiraiwa, Manabu
- Abstract
This paper presents MATLAB user interfaces for two multiphase kinetic models: the kinetic double-layer model of aerosol surface chemistry and gas--particle interactions (K2-SURF) and the kinetic multilayer model of aerosol surface and bulk chemistry (KM-SUB). Each interface has simple and user-friendly features that allow undergraduate and graduate students in physical, environmental, and atmospheric chemistry classes to learn about multiphase chemistry modeling without prior computer programming or modeling experience. It is easy to input parameters, and the simulation results are promptly displayed in the interface; thus, these model interfaces are particularly suitable for in-classroom and homework teaching applications. The model input parameters include surface and bulk reaction rate coefficients, surface accommodation coefficient, and gas and bulk diffusivities, while model outputs include gas uptake coefficient and surface and bulk concentrations. Students can use the K2-SURF interface to simulate surface processes and the KM-SUB interface to simulate surface and bulk processes. Example simulations were performed for each interface to present atmospherically relevant applications and to demonstrate its versatility for exploring model sensitivity on various kinetic parameters. The K2-SURF interface was used to show how the rate of ozone uptake by an organic surface and temporal evolution of surface concentrations are affected by the surface accommodation coefficient, desorption lifetime, and surface reaction rate coefficient. Additionally, the KM-SUB interface was applied to demonstrate how bulk diffusivities impact the degradation kinetics of oleic acid particles, so that students can learn how the phase state (liquid vs semisolid vs glassy solid) impacts multiphase chemical kinetics. The developed K2-SURF and KM-SUB interfaces are effective tools for modeling surface and bulk reactions in college-level educational settings, helping students to obtain a deeper understanding of the complex behaviors of heterogeneous and multiphase systems.
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- 2022
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3. Association of ischemic placental disease in a Southern California Birth Cohort and PM2.5 chemical species and oxidative potential markers
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Jonathan Liu, Qi Meng, Jiaqi Shen, Irish del Rosario, Pascale S J Lakey, Manabu Shiraiwa, Suzanne E Paulson, Scott Weichenthal, Yifang Zhu, Farzan Oroumiyeh, Carla Janzen, Sherin U Devaskar, Michael Jerrett, and Beate Ritz
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air pollution ,traffic ,ischemic placental disease ,metals ,PM2.5 ,Environmental sciences ,GE1-350 ,Public aspects of medicine ,RA1-1270 - Abstract
Road traffic is a significant source of particulate matter pollution, whose exposure is a significant risk factor in pregnancy-related health outcomes. The exact mechanisms behind the relationship between traffic-related air pollution (TRAP) exposure and adverse pregnancy outcomes remain unclear. We aim to assess the relationship between exposure to brake and tire wear-associated metals and oxidative potential and ischemic placental disease (IPD). Data were assembled from a final population of 178 women who sought specialized prenatal care at UCLA between 2016 and 2019 in Los Angeles, CA. Modeled first trimester exposures to chemical constituents and oxidative stress potential of PM _2.5 , black carbon, and PM _2.5 mass concentration. Speciated measurements included tracers of brake wear (barium), tire wear (zinc), and oxidative potential markers based on metal concentrations (KM-SUB-ELF ROS) or laboratory assays (DTT loss, OH radical formation). Exposures were modeled by integrating data from filter samples, a low-cost PM _2.5 sensor network, and land-use data. We used logistic regression to estimate the associations between air pollution exposures and IPD, adjusting for covariates assessed through medical records and interviews. Scaled to the interquartile range, odds ratios (95% CI) were as follows: barium OR: 1.7 (1.1, 2.7), zinc OR: 1.4 (.86, 2.4), and oxidative potential markers, both modeled as well as measured through DTT loss and OH formation assays (ORs ranging from 1.1-2.0). Point estimates of effect sizes for PM _2.5 and black carbon were lower than most measurements (ORs: 1.3-1.4). mass and black carbon. Our findings suggest two key points: (i) metals associated with brake and tire wear, currently unregulated, may play a role in the relationship between TRAP and adverse pregnancy outcomes, and (ii) reducing tailpipe emissions may not be sufficient to protect pregnant women from TRAP.
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- 2024
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4. Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii
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Emily V. Quach, Binh Cao, Edres Babacarkhial, Daniel Ho, Janak Sharma, and Pascale S. Guiton
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Phosphoglucomutase ,Glycolysis ,Gluconeogenesis ,Toxoplasma ,Amylopectin ,Tissue cysts ,Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Objective Toxoplasma gondii is a ubiquitous parasite of medical and veterinary importance; however, there exists no cure for chronic toxoplasmosis. Metabolic enzymes required for the production and maintenance of tissue cysts represent promising targets for novel therapies. Here, we use reverse genetics to investigate the role of Toxoplasma phosphoglucomutase 1, PGM1, in Toxoplasma growth and cystogenesis. Results We found that disruption of pgm1 did not significantly affect Toxoplasma intracellular growth and the lytic cycle. pgm1-defective parasites could differentiate into bradyzoites and produced cysts containing amylopectin in vitro. However, cysts produced in the absence of pgm1 were significantly smaller than wildtype. Together, our findings suggest that PGM1 is dispensable for in vitro growth but contributes to optimal Toxoplasma cyst development in vitro, thereby necessitating further investigation into the function of this enzyme in Toxoplasma persistence in its host.
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- 2022
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5. Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii
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Quach, Emily V., Cao, Binh, Babacarkhial, Edres, Ho, Daniel, Sharma, Janak, and Guiton, Pascale S.
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- 2022
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6. Elucidating gas–surface interactions relevant to atmospheric particle growth using combined temperature programmed desorption and temperature-dependent uptake.
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Johnson, Kristen N., Li, Yixin, Ezell, Michael J., Lakey, Pascale S. J., Shiraiwa, Manabu, and Finlayson-Pitts, Barbara J.
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Understanding growth mechanisms for particles in air is fundamental to developing a predictive capability for their impacts on human health, visibility, and climate. In the case of highly viscous semi-solid or solid particles, the likelihood of impinging gases being taken up to grow the particle will be influenced by the initial uptake coefficient and by the residence time of the adsorbed gas on the surface. Here, a new approach that combines Knudsen cell capabilities for gas uptake measurements with temperature programmed desorption (TPD) for binding energy measurements of gases is described. The application of this unique capability to the uptake of organic gases on silica demonstrates its utility and the combination of thermodynamic and kinetic data that can be obtained. Lower limits to the initial net uptake coefficients at 170 K are (3.0 ± 0.6) × 10
−3 , (4.9 ± 0.6) × 10−3 and (4.3 ± 0.8) × 10−3 for benzene, 1-chloropentane, and methanol, respectively, and are reported here for the first time. The uptake data demonstrated that the ideal gas lattice model was appropriate, which informed the analysis of the TPD data. From the thermal desorption measurements, desorption energies of 34.6 ± 2.5, 45.8 ± 5.5, and 40.0 ± 5.6 kJ mol−1 (errors are 1σ) are obtained for benzene, 1-chloropentane, and methanol, respectively, and show good agreement with previously reported measurements. A multiphase kinetics model was applied to quantify uptake, desorption, and diffusion through the particle multilayers and hence extract desorption kinetics. Implications for uptake of organics on silica surfaces in the atmosphere and the utility of this system for determining relationships between residence times of organic gases and particle surfaces of varying composition are discussed in the context of developing quantitative predictions for growth of aerosol particles in air. [ABSTRACT FROM AUTHOR]- Published
- 2024
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7. Weakening of the North American monsoon with global warming
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Pascale, S, Boos, WR, Bordoni, S, Delworth, TL, Kapnick, SB, Murakami, H, Vecchi, GA, and Zhang, W
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Atmospheric Sciences ,Physical Geography and Environmental Geoscience ,Environmental Science and Management - Abstract
Future changes in the North American monsoon, a circulation system that brings abundant summer rains to vast areas of the North American Southwest, could have significant consequences for regional water resources. How this monsoon will change with increasing greenhouse gases, however, remains unclear, not least because coarse horizontal resolution and systematic sea-surface temperature biases limit the reliability of its numerical model simulations. Here we investigate the monsoon response to increased atmospheric carbon dioxide (CO 2) concentrations using a 50-km-resolution global climate model which features a realistic representation of the monsoon climatology and its synoptic-scale variability. It is found that the monsoon response to CO 2 doubling is sensitive to sea-surface temperature biases. When minimizing these biases, the model projects a robust reduction in monsoonal precipitation over the southwestern United States, contrasting with previous multi-model assessments. Most of this precipitation decline can be attributed to increased atmospheric stability, and hence weakened convection, caused by uniform sea-surface warming. These results suggest improved adaptation measures, particularly water resource planning, will be required to cope with projected reductions in monsoon rainfall in the American Southwest.
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- 2017
8. Spatial and temporal scales of variability for indoor air constituents
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Pascale S. J. Lakey, Youngbo Won, David Shaw, Freja F. Østerstrøm, James Mattila, Emily Reidy, Brandon Bottorff, Colleen Rosales, Chen Wang, Laura Ampollini, Shan Zhou, Atila Novoselac, Tara F. Kahan, Peter F. DeCarlo, Jonathan P. D. Abbatt, Philip S. Stevens, Delphine K. Farmer, Nicola Carslaw, Donghyun Rim, and Manabu Shiraiwa
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Chemistry ,QD1-999 - Abstract
Concentration of air pollutants has generally been considered uniform in indoor environments. Here, the authors show that chlorinated cleaning products generate concentration gradients of pollutants due to photochemical reactions in sunlit areas, requiring a finer assessment of human exposure to these contaminants.
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- 2021
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9. Unexpected formation of oxygen-free products and nitrous acid from the ozonolysis of the neonicotinoid nitenpyram
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Wang, Weihong, Ezell, Michael J., Lakey, Pascale S. J., Aregahegn, Kifle Z., Shiraiwa, Manabu, and Finlayson-Pitts, Barbara J.
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- 2020
10. Biology and crop production in Space environments: Challenges and opportunities
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De Pascale, S., Arena, C., Aronne, G., De Micco, V., Pannico, A., Paradiso, R., and Rouphael, Y.
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- 2021
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11. An in vitro model of intestinal infection reveals a developmentally regulated transcriptome of Toxoplasma sporozoites and a NF-κB-like signature in infected host cells
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Guiton, Pascale S, Sagawa, Janelle M, Fritz, Heather M, and Boothroyd, John C
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Veterinary Sciences ,Agricultural ,Veterinary and Food Sciences ,Biomedical and Clinical Sciences ,Prevention ,Genetics ,Biodefense ,Emerging Infectious Diseases ,Infectious Diseases ,Vaccine Related ,Aetiology ,2.1 Biological and endogenous factors ,Infection ,Animals ,Cell Line ,Humans ,Intestinal Mucosa ,NF-kappa B ,Protozoan Proteins ,Rats ,Sporozoites ,Toxoplasma ,Toxoplasmosis ,Transcriptome ,Tumor Necrosis Factor-alpha ,General Science & Technology - Abstract
Toxoplasmosis is a zoonotic infection affecting approximately 30% of the world's human population. After sexual reproduction in the definitive feline host, Toxoplasma oocysts, each containing 8 sporozoites, are shed into the environment where they can go on to infect humans and other warm-blooded intermediate hosts. Here, we use an in vitro model to assess host transcriptomic changes that occur in the earliest stages of such infections. We show that infection of rat intestinal epithelial cells with mature sporozoites primarily results in higher expression of genes associated with Tumor Necrosis Factor alpha (TNFα) signaling via NF-κB. Furthermore, we find that, consistent with their biology, these mature, invaded sporozoites display a transcriptome intermediate between the previously reported day 10 oocysts and that of their tachyzoite counterparts. Thus, this study uncovers novel host and pathogen factors that may be critical for the establishment of a successful intracellular niche following sporozoite-initiated infection.
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- 2017
12. Transcriptional ups and downs: patterns of gene expression in the life cycle of Toxoplasma gondii
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Sharma, Janak, Rodriguez, Paula, Roy, Proyasha, and Guiton, Pascale S.
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- 2020
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13. Ozone Loss on Painted Surfaces: Dependence on Relative Humidity, Aging, and Exposure to Reactive SVOCs.
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Downey, Jillian P., Lakey, Pascale S. J., Shiraiwa, Manabu, and Abbatt, Jonathan P. D.
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- 2024
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14. Nilotinib in steroid-refractory cGVHD: prospective parallel evaluation of response, according to NIH criteria and exploratory response criteria (GITMO criteria)
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Olivieri, A., Mancini, G., Olivieri, J., Marinelli Busilacchi, E., Cimminiello, M., Pascale, S. P., Nuccorini, R., Patriarca, F., Corradini, P., Bacigalupo, A., Angelini, S., Poloni, A., Grillo, G., Onida, F., Martino, M., Di Renzo, N., Nagler, A., Mordini, N., Bruno, B., Ciceri, F., and Bonifazi, F.
- Published
- 2020
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15. Multiphase reactivity of polycyclic aromatic hydrocarbons is driven by phase separation and diffusion limitations
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Zhou, Shouming, Hwang, Brian C. H., Lakey, Pascale S. J., Zuend, Andreas, Abbatt, Jonathan P. D., and Shiraiwa, Manabu
- Published
- 2019
16. Spatial and temporal scales of variability for indoor air constituents
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Lakey, Pascale S. J., Won, Youngbo, Shaw, David, Østerstrøm, Freja F., Mattila, James, Reidy, Emily, Bottorff, Brandon, Rosales, Colleen, Wang, Chen, Ampollini, Laura, Zhou, Shan, Novoselac, Atila, Kahan, Tara F., DeCarlo, Peter F., Abbatt, Jonathan P. D., Stevens, Philip S., Farmer, Delphine K., Carslaw, Nicola, Rim, Donghyun, and Shiraiwa, Manabu
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- 2021
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17. Study success – A multilayer concept put under the microscope
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Petri Pascale S.
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Education - Abstract
In the ongoing debate about “Studierbarkeit”, the individual study process offers a promising starting point for institutional attempts to support study success. After briefly introducing established theoretical perspectives and empirical findings on the prediction of study success, I will argue for a closer alignment of research on and practical implementations of student support. Furthermore, I will present a process model of the – most critical – first year of study and demonstrate its fit using data from three cohorts. The practical implications derived from this model, as well as some strategic recommendations for how higher education institutions can foster study success are outlined.
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- 2021
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18. Role of microsatellite instability and HER2 positivity in locally advanced esophago-gastric cancer patients treated with peri-operative chemotherapy
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Gervaso, L, Bottiglieri, L, Meneses-Medina, M, Pellicori, S, Biffi, R, Fumagalli Romario, U, De Pascale, S, Sala, I, Bagnardi, V, Barberis, M, Cella, C, Fazio, N, Gervaso L., Bottiglieri L., Meneses-Medina M. I., Pellicori S., Biffi R., Fumagalli Romario U., De Pascale S., Sala I., Bagnardi V., Barberis M., Cella C. A., Fazio N., Gervaso, L, Bottiglieri, L, Meneses-Medina, M, Pellicori, S, Biffi, R, Fumagalli Romario, U, De Pascale, S, Sala, I, Bagnardi, V, Barberis, M, Cella, C, Fazio, N, Gervaso L., Bottiglieri L., Meneses-Medina M. I., Pellicori S., Biffi R., Fumagalli Romario U., De Pascale S., Sala I., Bagnardi V., Barberis M., Cella C. A., and Fazio N.
- Abstract
Purpose: Neoadjuvant chemotherapy (NAC) significantly improved the prognosis of patients with locally advanced gastric cancer (LAGC). Several biomarkers, including HER2 and MMR/MSI are crucial for treatment decisions in the advanced stage but, currently, no biomarkers can guide the choice of NAC in clinical practice. Our aim was to evaluate the role of MSI and HER2 status on clinical outcomes. Methods: We retrospectively collected LAGC patients treated with NAC and surgery +/- adjuvant chemotherapy from 2006 to 2018. HER2 and MSI were assessed on endoscopic and surgical samples. Pathologic complete response (pCR) rate, overall survival (OS), and event‐free survival (EFS) were estimated and evaluated for association with downstaging and MSI. Results: We included 76 patients, 8% were classified as MSI-H, entirely consistent between endoscopic and surgical samples. Six percent of patients were HER2 positive on endoscopic and 4% on surgical samples. Tumor downstaging was observed in 52.5% of cases, with three pCR (5.1%), none in MSI-H cancers. According to MSI status, event-free survival (EFS) and overall survival (OS) were higher for MSI-H patients to MSS [EFS not reached vs 30.0 months, p = 0.08; OS not reached vs 39.6 months, p = 0.10]. Conclusion: Our work confirms the positive prognostic effect of MSI-H in the curative setting of LAGC, not correlated with pathologic tumor downstaging. Prospective ad-hoc trial and tumor molecular profiling are eagerly needed.
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- 2023
19. Heterogeneous uptake of HO2 radicals onto atmospheric aerosols
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Matthews, Pascale S. J., Heard, Dwayne, and Beaza-Romero, Maria Teresa
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540 - Abstract
HO2 uptake coefficients were measured onto a variety of sub-micron aerosols and over a range of experimental conditions using an aerosol flow tube coupled with a sensitive Fluorescence Assay by Gas Expansion (FAGE) cell. Experiments showed that the deliquesced salt aerosols had larger HO2 uptake coefficients (γ = 0.003 – 0.016) than the effloresced salt aerosols (γ < 0.004). Similarly, solid organics had smaller uptake coefficients (γ < 0.004) than aqueous organics which were small (γ < 0.004 – γ = 0.008) unless metal ions were present. No observable dependence upon aerosol size or aerosol pH was measured for aqueous salt aerosols. The mass accommodation was measured as 0.5 ± 0.3 by doping the aerosols with copper. Measurements also showed that the HO2 uptake coefficient was highly dependent upon the copper and iron concentrations and increased between copper concentrations of 10-4 – 10-2 M within the aerosol. However, the addition of organics such as EDTA and oxalic acid to copper doped aerosols decreased the HO2 uptake coefficient by a factor of ~ 50 – 100. The HO2 uptake coefficient onto copper doped sucrose aerosols increased with increasing relative humidity. Secondary organic aerosols were generated in situ in a smog chamber and small uptake coefficients were measured onto α-pinene derived aerosols (γ < 0.001) and 1,3,5 trimethylbenzene derived aerosols (γ = 0.004 ± 0.002). Measurements onto Arizona Test Dust (ATD) aerosols showed much higher HO2 uptake coefficients (γ = 0.018 ± 0.006) than salt and organic aerosols at an initial HO2 concentration of 1 × 109 molecule cm-3 and increased with increasing humidity. Experiments were also performed over a temperature range of 263 -313 K onto effloresced sodium chloride and ammonium sulphate aerosols and onto deliquesced ammonium nitrate and copper doped ammonium nitrate. For the deliquesced ammonium nitrate aerosols the HO2 uptake coefficient increased with decreasing temperature. Finally, a time and apparent HO2 concentration dependence was observed for aqueous salt aerosols, copper doped aqueous aerosols and for ATD with larger HO2 uptake coefficients at shorter times and at lower HO2 concentrations. Modelling was performed using the kinetic multi-layer model of aerosol surface and bulk chemistry (KM-SUB) and showed that for undoped aqueous aerosols the time dependence could be explained by a decrease in the HO2 concentrations along the flow tube. The apparent HO2 concentration had the potential to be explained by a Fenton-like reaction whereby hydrogen peroxide exiting the injector was converted to HO2 within the aerosols, due to the presence of trace amounts of transition metal ions, which then partitioned back to the gas phase.
- Published
- 2014
20. Composition of indoor organic surface films in residences: simulating the influence of sources, partitioning, particle deposition, and air exchange.
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Cummings, Bryan E., Lakey, Pascale S. J., Morrison, Glenn C., Shiraiwa, Manabu, and Waring, Michael S.
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Indoor surfaces are coated with organic films that modulate thermodynamic interactions between the surfaces and room air. Recently published models can simulate film formation and growth via gas-surface partitioning, but none have statistically investigated film composition. The Indoor Model of Aerosols, Gases, Emissions, and Surfaces (IMAGES) was used here to simulate ten years of nonreactive film growth upon impervious indoor surfaces within a Monte Carlo procedure representing a sub-set of North American residential buildings. Film composition was resolved into categories reflecting indoor aerosol (gas + particle phases) factors from three sources: outdoor-originating, indoor-emitted, and indoor-generated secondary organic material. In addition to gas-to-film partitioning, particle deposition was modeled as a vector for organics to enter films, and it was responsible for a majority of the film mass after ∼1000 days of growth for the median simulation and is likely the main source of LVOCs within films. Therefore, the organic aerosol factor possessing the most SVOCs contributes most strongly to the composition of early films, but as the film ages, films become more dominated by the factor with the highest particle concentration. Indoor-emitted organics (e.g. from cooking) often constituted at least a plurality of the simulated mass in developed films, but indoor environments are diverse enough that any major organic material source could be the majority contributor to film mass, depending on building characteristics and indoor activities. A sensitivity analysis suggests that rapid film growth is most likely in both newer, more air-tight homes and older homes near primary pollution sources. [ABSTRACT FROM AUTHOR]
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- 2024
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21. Microscale vegetable production through microgreens: genotype, spectral bandwidth and substrate implications for growth and quality
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Rouphael, Y., primary, Ciriello, M., additional, Formisano, L., additional, El-Nakhel, C., additional, De Pascale, S., additional, Kyriacou, M.C., additional, and Caputo, R., additional
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- 2023
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22. PEERing forward in parasitology
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Absalon, Sabrina, primary, Cordy, Regina Joice, additional, Guiton, Pascale S., additional, Heaslip, Aoife, additional, and Smith, Joseph, additional
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- 2023
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23. Postharvest performance of cut rose cv. Lovely Red as affected by osmoprotectant and antitraspirant compounds
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Di Stasio, E., Rouphael, Y., Raimondi, G., El-Nakhel, C., and De Pascale, S.
- Published
- 2018
24. Recirculating aquaponics systems: the new era of food production
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Modarelli G. C., Cirillo C., Vanacore L., Langellotti A. L., Masi P., De Pascale S., Rouphael Y., Modarelli, G. C., Cirillo, C., Vanacore, L., Langellotti, A. L., Masi, P., De Pascale, S., and Rouphael, Y.
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plant factory with artificial lighting (PFAL) ,nutrient losse ,Horticulture ,integrated production system ,water quality - Abstract
To develop sustainable City/Region Food Systems (CRFS), a shift toward more ecofriendly and efficient production systems is needed. On its side, aquaponics systems rely on the equilibrium and interaction between fish, plants, and bacteria and are part of the so-called integrated production systems. Compared to other production techniques, it offers several advantages thanks to its management and operating costs feasibility. Recirculating Aquaponics Systems (RAS) provides the opportunity to finely close the nutrients loop inside an ecosystem and catch the best it can offer. Here we aim to highlight the strengths and weaknesses of RAS systems. In addition, we present as a case study the RAS prototype at the Department of Agricultural Sciences of University of Naples Federico II started in November 2020 to grow leafy vegetables and tilapia fish. Results provide helpful scientific and practical information for small and/or large-scale commercial growers as well as amateurs. Furthermore, our results highlight the suitability of these systems to be embedded in urban areas from rooftop and vertical farms to other controlled environment agricultural (CEA) systems and the opportunity they can offer for abandoned urban spaces.
- Published
- 2022
25. mSphere of Influence: No More Excuses—Addressing Race, Racism, and Socioeconomic Issues in the Science Classroom and Laboratory
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Pascale S. Guiton
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Microbiology ,QR1-502 - Abstract
Pascale Guiton works in the field of parasitology at a primarily undergraduate institution. In this mSphere of Influence article, she reflects on her difficulties as a faculty of color to discuss socioscientific issues in her classrooms. T.
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- 2021
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26. Quantifying the impact of relative humidity on human exposure to gas phase squalene ozonolysis products
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Pascale S. J. Lakey, Andreas Zuend, Glenn C. Morrison, Thomas Berkemeier, Jake Wilson, Caleb Arata, Allen H. Goldstein, Kevin R. Wilson, Nijing Wang, Jonathan Williams, Jonathan P. D. Abbatt, and Manabu Shiraiwa
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Chemistry (miscellaneous) ,Environmental Chemistry ,Pollution ,Analytical Chemistry - Abstract
Squalene is a major skin oil lipid which can react with ozone forming a range of products including carbonyls, carboxylic acids, hydroxy ketones, secondary ozonides and hydroperoxides. Previous experimental studies have shown that the yield of these products depends on relative humidity. A new mechanism is developed which treats the reaction of ozone with carbon–carbon double bonds and the subsequent reactions of the Criegee intermediates forming a range of products in both condensed and gas phases. The mechanism is included in kinetic models for a variety of ozonolysis reaction systems including pure squalene particles, squalene and skin oil films, clothing, and skin covered by clothing. The models reproduce experimental measurements reasonably well using relatively consistent parameters, providing insights into the important reactions and processes controlling the concentrations of different species. In general, gas-phase secondary carbonyl product concentrations increase significantly as a function of relative humidity due to their formation from the reaction of Criegee intermediates with water, while carboxylic acids and hydroxy ketone concentrations decrease. For example, human exposure to gas-phase 6-methyl-5-hepten-2-one can increase by approximately a factor of two as the relative humidity increases from 10% to 95%. The model can also reproduce the decay of carboxylic acids in skin oil due to their reaction with Criegee intermediates. Discrepancies between some of the measurements and model outputs indicate that in addition to impacting chemistry, relative humidity may affect other processes such as partitioning to surfaces and condensed-phase diffusion.
- Published
- 2023
27. Comparison of the Adsorption–Desorption Kinetics of Limonene and Carvone on TiO2 and SiO2 Surfaces under Different Relative Humidity Conditions
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Hanyu Fan, Pascale S. J. Lakey, Elianna S. Frank, Douglas J. Tobias, Manabu Shiraiwa, and Vicki H. Grassian
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General Energy ,Physical and Theoretical Chemistry ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2022
28. Microbial Community Dynamics and Response to Plant Growth-Promoting Microorganisms in the Rhizosphere of Four Common Food Crops Cultivated in Hydroponics
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Sheridan, C., Depuydt, P, De Ro, M., Petit, C., Van Gysegem, E., Delaere, P., Dixon, M., Stasiak, M., Aciksöz, S. B., Frossard, E., Paradiso, R., De Pascale, S., Ventorino, V., De Meyer, T., Sas, B., and Geelen, D.
- Published
- 2017
29. Photoenhanced Radical Formation in Aqueous Mixtures of Levoglucosan and Benzoquinone: Implications to Photochemical Aging of Biomass-Burning Organic Aerosols
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Gerritz, Lena, primary, Schervish, Meredith, additional, Lakey, Pascale S. J., additional, Oeij, Tim, additional, Wei, Jinlai, additional, Nizkorodov, Sergey A., additional, and Shiraiwa, Manabu, additional
- Published
- 2023
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30. Agronomical, physiological and water use efficiency changes of lettuce in response to deficit irrigation regimes
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Rouphael Y., De Pascale S., Karam F., Colla G., Cirillo C., Rouphael, Y., De Pascale, S., Karam, F., Colla, G., and Cirillo, C.
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deficit irrigation ,water use efficiency ,evapotranspiration ,Lactuca sativa L ,Horticulture ,crop coefficient - Abstract
Deficit irrigation occurrence while maintaining acceptable yield represents a useful tool for vegetables production wherever irrigation water is limited. Lettuce (Lactuca sativa L.) 'Corsica' was grown in the field from May to July in order (1) to investigate the daily and seasonal evapotranspiration (ETc) of lettuce using drainage lysimeter, (2) to determine the crop coefficients (Kc) values, and (3) to study the effects of deficit irrigation on growth, marketable and water use efficiency (WUE). Treatments were: well-watered treatment receiving 100% of ETc (C), water-stressed treatment receiving 80% of ETc (WS1), water-stressed treatment receiving 60% of ETc (WS2), water-stressed treatment receiving 40% of ETc (WS3). Lysimeter measured crop evapotranspiration (ETc) totaled 422 mm, for total irrigation period of 81 days. Seasonal evapotranspiration (ET) of lettuce treatments, varied from 351 mm in the WS1 treatment to 305 mm in the WS2 treatment and 259 mm in the WS3 treatment, while in the control ET totaled 397 mm. The highest yield was recorded in C and WS1, with no significant differences between treatments followed by WS2, while the lowest value was recorded under the severe water stress treatment. A yield response factor (ky) value of 1.17 was determined, and lettuce was found to be sensitive to water stress. The highest values of water use efficiency (WUE), was recorded on WS1 (18.8 kg m-3), followed by the C (17.8), WS2 (16.3) and finally WS3 (15.5) treatment. Applying 80% of ETc resulted in water saving, with the least yield reduction, making more water available to irrigate other crops, and thereby considered optimal strategies for drip-irrigated lettuce in the semi-arid climate.
- Published
- 2022
31. Gas Phase and Gas–Solid Interface Ozonolysis of Nitrogen Containing Alkenes: Nitroalkenes, Enamines, and Nitroenamines
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Weihong Wang, Xinke Wang, Pascale S. J. Lakey, Michael J. Ezell, Manabu Shiraiwa, and Barbara J. Finlayson-Pitts
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Nitrogen ,Molecular ,Alkenes ,Atomic ,Carbon ,Kinetics ,Ozone ,Particle and Plasma Physics ,Theoretical and Computational Chemistry ,Humans ,Nuclear ,Physical and Theoretical Chemistry ,Physical Chemistry (incl. Structural) - Abstract
Emerging contaminants are of concern due to their rapidly increasing numbers and potential ecological and human health effects. In this study, the synergistic effects of the presence of multifunctional nitro, amino and carbon-carbon double bond (C═C) groups on the gas phase ozonolysis in O2 or at the air/solid interface were investigated using five simple model compounds. The gas phase ozonolysis rate constants at 296 K were (3.5 ± 0.9) × 10-20 cm3 molecule-1 s-1 for 2-methyl-1-nitroprop-1-ene and (6.8 ± 0.8) × 10-19 cm3 molecule-1 s-1 for 4-methyl-4-nitro-1-pentene, with lifetimes of 134 and 7 days in the presence of 100 ppb ozone in the atmosphere, respectively. The rate constants for gas phase E-N,N-dimethyl-1-propenylamine and N,N-dimethylallylamine reactions with ozone were too fast (>10-18 cm3 molecule-1 s-1) to be measured, implying lifetimes of less than 5 days. A multiphase kinetics model (KM-GAP) was used to probe the gas-solid kinetics of 1-dimethylamino-2-nitroethylene, yielding a rate constant for the surface reaction of 1.8 × 10-9 cm2 molecule-1 s-1 and in the bulk 1× 10-16 cm3 molecule-1 s-1. These results show that a nitro group attached to the C═C lowers the gas phase rate constant by 2-3 orders of magnitude compared to the simple alkenes, while amino groups have the opposite effect. The presence of both groups provides counterbalancing effects. Products with deleterious health effects including dimethylformamide and formaldehyde were identified by FTIR. The identified products differentiate whether the initial site of ozone attack is C═C and/or the amino group. This study provides a basis for predicting the environmental fates of emerging contaminants and shows that both the toxicity of both the parent compounds and the products should be taken into account in assessing their environmental impacts.
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- 2022
32. Chemical and Cellular Formation of Reactive Oxygen Species from Secondary Organic Aerosols in Epithelial Lining Fluid.
- Author
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Shiraiwa, Manabu, Ting Fang, Jinlai Wei, Lakey, Pascale S. J., Hwang, Brian C. H., Edwards, Kasey C., Kapur, Sukriti, Mena, Jessica E. Monterrosa, Yu-Kai Huang, Digman, Michelle A., Weichenthal, Scott A., Nizkorodov, Sergey, and Kleinman, Michael T.
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- 2023
33. PEERing forward in parasitology
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Sabrina Absalon, Regina Joice Cordy, Pascale S. Guiton, Aoife Heaslip, and Joseph Smith
- Subjects
Infectious Diseases ,Parasitology - Published
- 2023
34. The differential modulation of secondary metabolism induced by a protein hydrolysate and a seaweed extract in tomato plants under salinity
- Author
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Zhang L., Freschi G., Rouphael Y., De Pascale S., Lucini L., Zhang, L., Freschi, G., Rouphael, Y., De Pascale, S., and Lucini, L.
- Subjects
phytohormones ,biostimulants ,secondary metabolism ,plant stre ,plant stress ,Settore AGR/13 - CHIMICA AGRARIA ,Plant Science ,phytohormone ,biostimulant ,metabolomics ,metabolomic - Abstract
Climate change and abiotic stress challenges in crops are threatening world food production. Among others, salinity affects the agricultural sector by significantly impacting yield losses. Plant biostimulants have received increasing attention in the agricultural industry due to their ability to improve health and resilience in crops. The main driving force of these products lies in their ability to modulate plant metabolic processes involved in the stress response. This study’s purpose was to investigate the effect of two biostimulant products, including a protein hydrolysate (Clever HX®) and a seaweed extract with high amino acids content (Ascovip®), and their combination, on the metabolomics profile of tomato crops grown under salt stress (150 mM NaCl). Several stress indicators (leaf relative water content, membrane stability index, and photosynthesis activity) and leaf mineral composition after salinity stress exposure were assessed to evaluate stress mitigation, together with growth parameters (shoot and root biomasses). After that, an untargeted metabolomics approach was used to investigate the mechanism of action of the biostimulants and their link with the increased resilience to stress. The application of the biostimulants used reduced the detrimental effect of salinity. In saline conditions, protein hydrolysate improved shoot dry weight while seaweed extracts improved root dry weight. Regarding stress indicators, the application of the protein hydrolysate was found to alleviate the membrane damage caused by salinity stress compared to untreated plants. Surprisingly, photosynthetic activity significantly improved after treatment with seaweed extracts, suggesting a close correlation between root development, root water assimilation capacity and photosynthetic activity. Considering the metabolic reprogramming after plant biostimulants application, protein hydrolysates and their combination with seaweed extracts reported a distinctive metabolic profile modulation, mainly in secondary metabolite, lipids and fatty acids, and phytohormones biosynthetic pathways. However, treatment with seaweed extract reported a similar metabolic reprogramming trend compared to salinity stress. Our findings indicate a different mechanism of action modulated by protein hydrolysate and seaweed extract, suggesting stronger activity as a stress mitigator of protein hydrolysate in tomato crops under salinity stress.
- Published
- 2023
35. Open versus laparoscopic gastrectomy for advanced gastric cancer: a propensity score matching analysis of survival in a western population—on behalf of the Italian Research Group for Gastric Cancer
- Author
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Lombardi, P, Bernasconi, D, Baiocchi, G, Berselli, M, Biondi, A, Castoro, C, Catarci, M, Degiuli, M, Fumagalli Romario, U, Giacopuzzi, S, Marchesi, F, Marrelli, D, Mazzola, M, Molfino, S, Olmi, S, Rausei, S, Rosa, F, Rosati, R, Roviello, F, Santi, S, Solaini, L, Staderini, F, Vigano, J, Ferrari, G, Agnes, A, Alfieri, S, Alloggio, M, Bencivenga, M, Benedetti, M, Bottari, A, Cianchi, F, Cocozza, E, Dalmonte, G, De Martini, P, De Pascale, S, Desio, M, Emiliani, G, Ercolani, G, Galli, F, Garosio, I, Giani, A, Gualtierotti, M, Marano, L, Morgagni, P, Peri, A, Puccetti, F, Reddavid, R, Uccelli, M, Lombardi P. M., Bernasconi D., Baiocchi G. L., Berselli M., Biondi A., Castoro C., Catarci M., Degiuli M., Fumagalli Romario U., Giacopuzzi S., Marchesi F., Marrelli D., Mazzola M., Molfino S., Olmi S., Rausei S., Rosa F., Rosati R., Roviello F., Santi S., Solaini L., Staderini F., Vigano J., Ferrari G., Agnes A., Alfieri S., Alloggio M., Bencivenga M., Benedetti M., Bottari A., Cianchi F., Cocozza E., Dalmonte G., De Martini P., De Pascale S., Desio M., Emiliani G., Ercolani G., Galli F., Garosio I., Giani A., Gualtierotti M., Marano L., Morgagni P., Peri A., Puccetti F., Reddavid R., Uccelli M., Lombardi, P, Bernasconi, D, Baiocchi, G, Berselli, M, Biondi, A, Castoro, C, Catarci, M, Degiuli, M, Fumagalli Romario, U, Giacopuzzi, S, Marchesi, F, Marrelli, D, Mazzola, M, Molfino, S, Olmi, S, Rausei, S, Rosa, F, Rosati, R, Roviello, F, Santi, S, Solaini, L, Staderini, F, Vigano, J, Ferrari, G, Agnes, A, Alfieri, S, Alloggio, M, Bencivenga, M, Benedetti, M, Bottari, A, Cianchi, F, Cocozza, E, Dalmonte, G, De Martini, P, De Pascale, S, Desio, M, Emiliani, G, Ercolani, G, Galli, F, Garosio, I, Giani, A, Gualtierotti, M, Marano, L, Morgagni, P, Peri, A, Puccetti, F, Reddavid, R, Uccelli, M, Lombardi P. M., Bernasconi D., Baiocchi G. L., Berselli M., Biondi A., Castoro C., Catarci M., Degiuli M., Fumagalli Romario U., Giacopuzzi S., Marchesi F., Marrelli D., Mazzola M., Molfino S., Olmi S., Rausei S., Rosa F., Rosati R., Roviello F., Santi S., Solaini L., Staderini F., Vigano J., Ferrari G., Agnes A., Alfieri S., Alloggio M., Bencivenga M., Benedetti M., Bottari A., Cianchi F., Cocozza E., Dalmonte G., De Martini P., De Pascale S., Desio M., Emiliani G., Ercolani G., Galli F., Garosio I., Giani A., Gualtierotti M., Marano L., Morgagni P., Peri A., Puccetti F., Reddavid R., and Uccelli M.
- Abstract
Background: Oncologic outcomes after laparoscopic gastrectomy for advanced gastric cancer in the West have been poorly investigated. The aim of the present study was to compare survival outcomes in patients undergoing curative-intent laparoscopic and open gastrectomy for advanced gastric cancer in several centres belonging to the Italian Research Group for Gastric Cancer. Methods: Data of patients operated between 2015 and 2018 were retrospectively analysed. Propensity Score Matching was performed to balance baseline characteristics of patients undergoing laparoscopic and open gastrectomy. The primary endpoint was 3-year overall survival. Secondary endpoints were 3-year disease-free survival and short-term outcomes. Multivariable regression analyses for survival were conducted. Results: Data were retrieved from 20 centres. Of the 717 patients included, 438 patients were correctly matched, 219 per group. The 3-year overall survival was 73.6% and 68.7% in the laparoscopic and open group, respectively (p = 0.40). When compared with open gastrectomy, laparoscopic gastrectomy showed comparable 3-year disease-free survival (62.8%, vs 58.9%, p = 0.40), higher rate of return to intended oncologic treatment (56.9% vs 40.2%, p = 0.001), similar 30-day morbidity/mortality. Prognostic factors for survival were ASA Score ≥ 3, age-adjusted Charlson Comorbidity Index ≥ 5, lymph node ratio ≥ 0.15, p/ypTNM Stage III and return to intended oncologic treatment. Conclusions: Laparoscopic gastrectomy for advanced gastric cancer offers similar rates of survival when compared to open gastrectomy, with higher rates of return to intended oncologic treatment. ASA score, age-adjusted Charlson Comorbidity Index, lymph node ratio, return to intended oncologic treatment and p/ypTNM Stage, but not surgical approach, are prognostic factors for survival.
- Published
- 2022
36. Current practice on the use of prophylactic drain after gastrectomy in Italy: the Abdominal Drain in Gastrectomy (ADiGe) survey
- Author
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Mengardo, V, Weindelmayer, J, Veltri, A, Giacopuzzi, S, Torroni, L, de Manzoni, G, Agresta, F, Alfieri, R, Alfieri, S, Antonacci, N, Baiocchi, G, Bencini, L, Bencivenga, M, Benedetti, M, Berselli, M, Biondi, A, Capolupo, G, Carboni, F, Casadei, R, Casella, F, Catarci, M, Cerri, P, Chiari, D, Cocozza, E, Colombo, G, Cozzaglio, L, Dalmonte, G, Degiuli, M, De Luca, M, De Luca, R, De Manzini, N, De Pasqual, C, De Pascale, S, De Ruvo, N, Di Cosmo, M, Di Leo, A, Di Paola, M, Elio, A, Ferrara, F, Ferrari, G, Fiscon, V, Fumagalli, U, Garulli, G, Gennai, A, Gentile, I, Germani, P, Gualtierotti, M, Guerini, F, Gurrado, A, Inama, M, La Torre, F, Laterza, E, Losurdo, P, Macri, A, Marano, A, Marano, L, Marchesi, F, Marino, F, Massani, M, Menghi, R, Milone, M, Molfino, S, Montuori, M, Moretto, G, Morgagni, P, Morpurgo, E, Abdallah, M, Nespoli, L, Olmi, S, Palaia, R, Pallabazer, G, Parise, P, Pasculli, A, Pericoli Ridolfini, M, Pesce, A, Pinotti, E, Pisano, M, Poiasina, E, Postiglione, V, Rausei, S, Rella, A, Rosa, F, Rosati, R, Rossi, G, Rossit, L, Rovatti, M, Ruspi, L, Sacco, L, Saladino, E, Sansonetti, A, Sartori, A, Scaglione, D, Scaringi, S, Schoenthaler, C, Sena, G, Simone, M, Solaini, L, Strignano, P, Tartaglia, N, Testa, S, Testini, M, Tiberio, G, Treppiedi, E, Vagliasindi, A, Valmasoni, M, Vigano, J, Zanchettin, G, Zanoni, A, Zardini, C, Zerbinati, A, Mengardo V., Weindelmayer J., Veltri A., Giacopuzzi S., Torroni L., de Manzoni G., Agresta F., Alfieri R., Alfieri S., Antonacci N., Baiocchi G. L., Bencini L., Bencivenga M., Benedetti M., Berselli M., Biondi A., Capolupo G. T., Carboni F., Casadei R., Casella F., Catarci M., Cerri P., Chiari D., Cocozza E., Colombo G., Cozzaglio L., Dalmonte G., Degiuli M., De Luca M., De Luca R., De Manzini N., De Pasqual C. A., De Pascale S., De Ruvo N., Di Cosmo M., Di Leo A., Di Paola M., Elio A., Ferrara F., Ferrari G., Fiscon V., Fumagalli U., Garulli G., Gennai A., Gentile I., Germani P., Gualtierotti M., Guerini F., Gurrado A., Inama M., La Torre F., Laterza E., Losurdo P., Macri A., Marano A., Marano L., Marchesi F., Marino F., Massani M., Menghi R., Milone M., Molfino S., Montuori M., Moretto G., Morgagni P., Morpurgo E., Abdallah M., Nespoli L., Olmi S., Palaia R., Pallabazer G., Parise P., Pasculli A., Pericoli Ridolfini M., Pesce A., Pinotti E., Pisano M., Poiasina E., Postiglione V., Rausei S., Rella A., Rosa F., Rosati R., Rossi G., Rossit L., Rovatti M., Ruspi L., Sacco L., Saladino E., Sansonetti A., Sartori A., Scaglione D., Scaringi S., Schoenthaler C., Sena G., Simone M., Solaini L., Strignano P., Tartaglia N., Testa S., Testini M., Tiberio G. A. M., Treppiedi E., Vagliasindi A., Valmasoni M., Vigano J., Zanchettin G., Zanoni A., Zardini C., Zerbinati A., Mengardo, V, Weindelmayer, J, Veltri, A, Giacopuzzi, S, Torroni, L, de Manzoni, G, Agresta, F, Alfieri, R, Alfieri, S, Antonacci, N, Baiocchi, G, Bencini, L, Bencivenga, M, Benedetti, M, Berselli, M, Biondi, A, Capolupo, G, Carboni, F, Casadei, R, Casella, F, Catarci, M, Cerri, P, Chiari, D, Cocozza, E, Colombo, G, Cozzaglio, L, Dalmonte, G, Degiuli, M, De Luca, M, De Luca, R, De Manzini, N, De Pasqual, C, De Pascale, S, De Ruvo, N, Di Cosmo, M, Di Leo, A, Di Paola, M, Elio, A, Ferrara, F, Ferrari, G, Fiscon, V, Fumagalli, U, Garulli, G, Gennai, A, Gentile, I, Germani, P, Gualtierotti, M, Guerini, F, Gurrado, A, Inama, M, La Torre, F, Laterza, E, Losurdo, P, Macri, A, Marano, A, Marano, L, Marchesi, F, Marino, F, Massani, M, Menghi, R, Milone, M, Molfino, S, Montuori, M, Moretto, G, Morgagni, P, Morpurgo, E, Abdallah, M, Nespoli, L, Olmi, S, Palaia, R, Pallabazer, G, Parise, P, Pasculli, A, Pericoli Ridolfini, M, Pesce, A, Pinotti, E, Pisano, M, Poiasina, E, Postiglione, V, Rausei, S, Rella, A, Rosa, F, Rosati, R, Rossi, G, Rossit, L, Rovatti, M, Ruspi, L, Sacco, L, Saladino, E, Sansonetti, A, Sartori, A, Scaglione, D, Scaringi, S, Schoenthaler, C, Sena, G, Simone, M, Solaini, L, Strignano, P, Tartaglia, N, Testa, S, Testini, M, Tiberio, G, Treppiedi, E, Vagliasindi, A, Valmasoni, M, Vigano, J, Zanchettin, G, Zanoni, A, Zardini, C, Zerbinati, A, Mengardo V., Weindelmayer J., Veltri A., Giacopuzzi S., Torroni L., de Manzoni G., Agresta F., Alfieri R., Alfieri S., Antonacci N., Baiocchi G. L., Bencini L., Bencivenga M., Benedetti M., Berselli M., Biondi A., Capolupo G. T., Carboni F., Casadei R., Casella F., Catarci M., Cerri P., Chiari D., Cocozza E., Colombo G., Cozzaglio L., Dalmonte G., Degiuli M., De Luca M., De Luca R., De Manzini N., De Pasqual C. A., De Pascale S., De Ruvo N., Di Cosmo M., Di Leo A., Di Paola M., Elio A., Ferrara F., Ferrari G., Fiscon V., Fumagalli U., Garulli G., Gennai A., Gentile I., Germani P., Gualtierotti M., Guerini F., Gurrado A., Inama M., La Torre F., Laterza E., Losurdo P., Macri A., Marano A., Marano L., Marchesi F., Marino F., Massani M., Menghi R., Milone M., Molfino S., Montuori M., Moretto G., Morgagni P., Morpurgo E., Abdallah M., Nespoli L., Olmi S., Palaia R., Pallabazer G., Parise P., Pasculli A., Pericoli Ridolfini M., Pesce A., Pinotti E., Pisano M., Poiasina E., Postiglione V., Rausei S., Rella A., Rosa F., Rosati R., Rossi G., Rossit L., Rovatti M., Ruspi L., Sacco L., Saladino E., Sansonetti A., Sartori A., Scaglione D., Scaringi S., Schoenthaler C., Sena G., Simone M., Solaini L., Strignano P., Tartaglia N., Testa S., Testini M., Tiberio G. A. M., Treppiedi E., Vagliasindi A., Valmasoni M., Vigano J., Zanchettin G., Zanoni A., Zardini C., and Zerbinati A.
- Abstract
Evidence against the use of prophylactic drain after gastrectomy are increasing and ERAS guidelines suggest the benefit of drain avoidance. Nevertheless, it is unclear whether this practice is still widespread. We conducted a survey among Italian surgeons through the Italian Gastric Cancer Research Group and the Polispecialistic Society of Young Surgeons, aiming to understand the current use of prophylactic drain. A 28-item questionnaire-based survey was developed to analyze the current practice and the individual opinion about the use of prophylactic drain after gastrectomy. Groups based on age, experience and unit volume were separately analyzed. Response of 104 surgeons from 73 surgical units were collected. A standardized ERAS protocol for gastrectomy was applied by 42% of the respondents. Most of the surgeons, regardless of age, experience, or unit volume, declared to routinely place one or more drain after gastrectomy. Only 2 (1.9%) and 7 surgeons (6.7%) belonging to high volume units, do not routinely place drains after total and subtotal gastrectomy, respectively. More than 60% of the participants remove the drain on postoperative day 4–6 after performing an assessment of the anastomosis integrity. Interestingly, less than half of the surgeons believe that drain is the main tool for leak management, and this percentage further drops among younger surgeons. On the other hand, drain’s role seems to be more defined for duodenal stump leak treatment, with almost 50% of the surgeons recognizing its importance. Routine use of prophylactic drain after gastrectomy is still a widespread practice even if younger surgeons are more persuaded that it could not be advantageous.
- Published
- 2022
37. Endoscopic submucosal dissection for gastric superficial neoplastic lesions: a single center experience
- Author
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Soru, P., additional, Bravi, I., additional, De Roberto, G., additional, Fumagalli, U., additional, De Pascale, S., additional, Crosta, C., additional, Giunta, M., additional, Maregatti, M., additional, Ravizza, D., additional, Trovato, C., additional, and Fiori, G., additional
- Published
- 2023
- Full Text
- View/download PDF
38. Feasibility, safety and efficacy of partially covered self-expandable metal stents for leaks after esophageal or gastric resection for malignances: a single center experience
- Author
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Bravi, I., additional, Soru, P., additional, Roberto, G. De, additional, Fumagalli, U., additional, Pascale, S. De, additional, Crosta, C., additional, Giunta, M., additional, Ravizza, D., additional, Trovato, C., additional, and Fiori, G., additional
- Published
- 2023
- Full Text
- View/download PDF
39. The differential modulation of secondary metabolism induced by a protein hydrolysate and a seaweed extract in tomato plants under salinity
- Author
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Zhang, Leilei, Freschi, G., Rouphael, Y., De Pascale, S., Lucini, Luigi, Zhang L., Lucini L. (ORCID:0000-0002-5133-9464), Zhang, Leilei, Freschi, G., Rouphael, Y., De Pascale, S., Lucini, Luigi, Zhang L., and Lucini L. (ORCID:0000-0002-5133-9464)
- Abstract
Climate change and abiotic stress challenges in crops are threatening world food production. Among others, salinity affects the agricultural sector by significantly impacting yield losses. Plant biostimulants have received increasing attention in the agricultural industry due to their ability to improve health and resilience in crops. The main driving force of these products lies in their ability to modulate plant metabolic processes involved in the stress response. This study’s purpose was to investigate the effect of two biostimulant products, including a protein hydrolysate (Clever HX®) and a seaweed extract with high amino acids content (Ascovip®), and their combination, on the metabolomics profile of tomato crops grown under salt stress (150 mM NaCl). Several stress indicators (leaf relative water content, membrane stability index, and photosynthesis activity) and leaf mineral composition after salinity stress exposure were assessed to evaluate stress mitigation, together with growth parameters (shoot and root biomasses). After that, an untargeted metabolomics approach was used to investigate the mechanism of action of the biostimulants and their link with the increased resilience to stress. The application of the biostimulants used reduced the detrimental effect of salinity. In saline conditions, protein hydrolysate improved shoot dry weight while seaweed extracts improved root dry weight. Regarding stress indicators, the application of the protein hydrolysate was found to alleviate the membrane damage caused by salinity stress compared to untreated plants. Surprisingly, photosynthetic activity significantly improved after treatment with seaweed extracts, suggesting a close correlation between root development, root water assimilation capacity and photosynthetic activity. Considering the metabolic reprogramming after plant biostimulants application, protein hydrolysates and their combination with seaweed extracts reported a distinctive metabolic profile modula
- Published
- 2023
40. A national survey on the current status of minimally invasive gastric practice on behalf of GIRCG
- Author
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Milone, M, D'Amore, A, Alfieri, S, Ambrosio, M, Andreuccetti, J, Ansaloni, L, Antonucci, A, Arganini, M, Baiocchi, G, Barone, M, Bencini, L, Bencivenga, M, Boccia, L, Boni, L, Braga, M, Cianchi, F, Cipollari, C, Contine, A, Cotsoglou, C, D'Imporzano, S, De Manzoni, G, De Pascale, S, De Ruvo, N, Degiuli, M, Donini, A, Elmore, U, Ercolani, G, Ferrari, G, Fumagalli, R, Garulli, G, Gelmini, R, Graziosi, L, Gualtierotti, M, Guglielmi, A, Inama, M, Maffeis, F, Maione, F, Manigrasso, M, Marchesi, F, Marrelli, D, Massobrio, A, Moretto, G, Moukachar, A, Navarra, G, Nigri, G, Olmi, S, Palaia, R, Papis, D, Parise, P, Pedrazzani, C, Petri, R, Pignata, G, Pisano, M, Rausei, S, Reddavid, R, Rocco, G, Rosa, F, Rosati, R, Rossit, L, Rottoli, M, Roviello, F, Santi, S, Scabini, S, Scaringi, S, Solaini, L, Staderini, F, Taglietti, L, Torre, B, Ubiali, P, Uccelli, M, Uggeri, F, Vertaldi, S, Viganò, J, De Palma, G, Giacopuzzi, S, Milone, Marco, D'Amore, Anna, Alfieri, Sergio, Ambrosio, Maria Raffaella, Andreuccetti, Jacopo, Ansaloni, Luca, Antonucci, Adelmo, Arganini, Marco, Baiocchi, Gianluca, Barone, Mirko, Bencini, Lapo, Bencivenga, Maria, Boccia, Luigi, Boni, Luigi, Braga, Marco, Cianchi, Fabio, Cipollari, Chiara, Contine, Alessandro, Cotsoglou, Christian, D'Imporzano, Simone, De Manzoni, Giovanni, De Pascale, Stefano, De Ruvo, Nicola, Degiuli, Maurizio, Donini, Annibale, Elmore, Ugo, Ercolani, Giorgio, Ferrari, Giovanni, Fumagalli, Romario Uberto, Garulli, Gianluca, Gelmini, Roberta, Graziosi, Luigina, Gualtierotti, Monica, Guglielmi, Alfredo, Inama, Marco, Maffeis, Federica, Maione, Francesco, Manigrasso, Michele, Marchesi, Federico, Marrelli, Daniele, Massobrio, Andrea, Moretto, Gianluigi, Moukachar, Aballah, Navarra, Giuseppe, Nigri, Giuseppe, Olmi, Stefano, Palaia, Raffaele, Papis, Davide, Parise, Paolo, Pedrazzani, Corrado, Petri, Roberto, Pignata, Giusto, Pisano, Michele, Rausei, Stefano, Reddavid, Rossella, Rocco, Giuseppe, Rosa, Fausto, Rosati, Riccardo, Rossit, Luca, Rottoli, Matteo, Roviello, Franco, Santi, Stefano, Scabini, Stefano, Scaringi, Stefano, Solaini, Leonardo, Staderini, Fabio, Taglietti, Lucio, Torre, Beatrice, Ubiali, Paolo, Uccelli, Matteo, Uggeri, Fabio, Vertaldi, Sara, Viganò, Jacopo, De Palma, Giovanni Domenico, Giacopuzzi, Simone, Milone, M, D'Amore, A, Alfieri, S, Ambrosio, M, Andreuccetti, J, Ansaloni, L, Antonucci, A, Arganini, M, Baiocchi, G, Barone, M, Bencini, L, Bencivenga, M, Boccia, L, Boni, L, Braga, M, Cianchi, F, Cipollari, C, Contine, A, Cotsoglou, C, D'Imporzano, S, De Manzoni, G, De Pascale, S, De Ruvo, N, Degiuli, M, Donini, A, Elmore, U, Ercolani, G, Ferrari, G, Fumagalli, R, Garulli, G, Gelmini, R, Graziosi, L, Gualtierotti, M, Guglielmi, A, Inama, M, Maffeis, F, Maione, F, Manigrasso, M, Marchesi, F, Marrelli, D, Massobrio, A, Moretto, G, Moukachar, A, Navarra, G, Nigri, G, Olmi, S, Palaia, R, Papis, D, Parise, P, Pedrazzani, C, Petri, R, Pignata, G, Pisano, M, Rausei, S, Reddavid, R, Rocco, G, Rosa, F, Rosati, R, Rossit, L, Rottoli, M, Roviello, F, Santi, S, Scabini, S, Scaringi, S, Solaini, L, Staderini, F, Taglietti, L, Torre, B, Ubiali, P, Uccelli, M, Uggeri, F, Vertaldi, S, Viganò, J, De Palma, G, Giacopuzzi, S, Milone, Marco, D'Amore, Anna, Alfieri, Sergio, Ambrosio, Maria Raffaella, Andreuccetti, Jacopo, Ansaloni, Luca, Antonucci, Adelmo, Arganini, Marco, Baiocchi, Gianluca, Barone, Mirko, Bencini, Lapo, Bencivenga, Maria, Boccia, Luigi, Boni, Luigi, Braga, Marco, Cianchi, Fabio, Cipollari, Chiara, Contine, Alessandro, Cotsoglou, Christian, D'Imporzano, Simone, De Manzoni, Giovanni, De Pascale, Stefano, De Ruvo, Nicola, Degiuli, Maurizio, Donini, Annibale, Elmore, Ugo, Ercolani, Giorgio, Ferrari, Giovanni, Fumagalli, Romario Uberto, Garulli, Gianluca, Gelmini, Roberta, Graziosi, Luigina, Gualtierotti, Monica, Guglielmi, Alfredo, Inama, Marco, Maffeis, Federica, Maione, Francesco, Manigrasso, Michele, Marchesi, Federico, Marrelli, Daniele, Massobrio, Andrea, Moretto, Gianluigi, Moukachar, Aballah, Navarra, Giuseppe, Nigri, Giuseppe, Olmi, Stefano, Palaia, Raffaele, Papis, Davide, Parise, Paolo, Pedrazzani, Corrado, Petri, Roberto, Pignata, Giusto, Pisano, Michele, Rausei, Stefano, Reddavid, Rossella, Rocco, Giuseppe, Rosa, Fausto, Rosati, Riccardo, Rossit, Luca, Rottoli, Matteo, Roviello, Franco, Santi, Stefano, Scabini, Stefano, Scaringi, Stefano, Solaini, Leonardo, Staderini, Fabio, Taglietti, Lucio, Torre, Beatrice, Ubiali, Paolo, Uccelli, Matteo, Uggeri, Fabio, Vertaldi, Sara, Viganò, Jacopo, De Palma, Giovanni Domenico, and Giacopuzzi, Simone
- Abstract
Italian Research Group for Gastric Cancer (GIRCG), during the 2013 annual Consensus Conference to gastric cancer, stated that laparoscopic or robotic approach should be limited only to early gastric cancer (EGC) and no further guidelines were currently available. However, accumulated evidences, mainly from eastern experiences, have supported the application of minimally invasive surgery also for locally advanced gastric cancer (AGC). The aim of our study is to give a snapshot of current surgical propensity of expert Italian upper gastrointestinal surgeons in performing minimally invasive techniques for the treatment of gastric cancer in order to answer to the question if clinical practice overcome the recommendation. Experts in the field among the Italian Research Group for Gastric Cancer (GIRCG) were invited to join a web 30-item survey through a formal e-mail from January 1st, 2020, to June 31st, 2020. Responses were collected from 46 participants out of 100 upper gastrointestinal surgeons. Percentage of surgeons choosing a minimally invasive approach to treat early and advanced gastric cancer was similar. Additionally analyzing data from the centers involved, we obtained that the percentage of minimally invasive total and partial gastrectomies in advanced cases augmented with the increase of surgical procedures performed per year (p=0.02 and p=0.04 respectively). It is reasonable to assume that there is a widening of indications given by the current national guideline into clinical practice. Propensity of expert Italian upper gastrointestinal surgeons was to perform minimally invasive surgery not only for early but also for advanced gastric cancer. Of interest volume activity correlated with the propensity of surgeons to select a minimally invasive approach.
- Published
- 2023
41. Zinc biofortification of hydroponically grown basil: Stress physiological responses and impact on antioxidant secondary metabolites of genotypic variants
- Author
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Ciriello M., Formisano L., Kyriacou M., Soteriou G. A., Graziani G., De Pascale S., Rouphael Y., Ciriello, M., Formisano, L., Kyriacou, M., Soteriou, G. A., Graziani, G., De Pascale, S., and Rouphael, Y.
- Subjects
floating system ,pigment ,Ocimum basilicumL ,UHPLC ,phenolic ,Plant Science ,Zn agronomic biofortification - Abstract
Ocimum basilicum L. is an aromatic plant rich in bioactive metabolites beneficial to human health. The agronomic biofortification of basil with Zn could provide a practical and sustainable solution to address Zn deficiency in humans. Our research appraised the effects of biofortification implemented through nutrient solutions of different Zn concentration (12.5, 25.0, 37.5, and 50 µM) on the yield, physiological indices (net CO2 assimilation rate, transpiration, stomatal conductance, and chlorophyll fluorescence), quality, and Zn concentration of basil cultivars ‘Aroma 2’ and ‘Eleonora’ grown in a floating raft system. The ABTS, DPPH, and FRAP antioxidant activities were determined by UV-VIS spectrophotometry, the concentrations of phenolic acids by mass spectrometry using a Q Extractive Orbitrap LC-MS/MS, and tissue Zn concentration by inductively coupled plasma mass spectrometry. Although increasing the concentration of Zn in the nutrient solution significantly reduced the yield, this reduction was less evident in ‘Aroma 2’. However, regardless of cultivar, the use of the maximum dose of Zn (50 µM) increased the concentration of carotenoids, polyphenols, and antioxidant activity on average by 19.76, 14.57, and 33.72%, respectively, compared to the Control. The significant positive correlation between Zn in the nutrient solution and Zn in plant tissues underscores the suitability of basil for soilless biofortification programs.
- Published
- 2022
42. Heterogeneous Interactions between Carvone and Hydroxylated SiO2
- Author
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Hanyu Fan, Elianna S. Frank, Pascale S. J. Lakey, Manabu Shiraiwa, Douglas J. Tobias, and Vicki H. Grassian
- Subjects
General Energy ,Physical and Theoretical Chemistry ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2022
43. Multiphase Kinetic Multilayer Model Interfaces for Simulating Surface and Bulk Chemistry for Environmental and Atmospheric Chemistry Teaching
- Author
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Amy K. Hua, Pascale S. J. Lakey, and Manabu Shiraiwa
- Subjects
General Chemistry ,Education - Published
- 2022
44. The impact of clothing on ozone and squalene ozonolysis products in indoor environments
- Author
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Lakey, Pascale S. J., Morrison, Glenn C., Won, Youngbo, Parry, Krista M., von Domaros, Michael, Tobias, Douglas J., Rim, Donghyun, and Shiraiwa, Manabu
- Published
- 2019
- Full Text
- View/download PDF
45. Fine Particulate Matter Metal Composition, Oxidative Potential, and Adverse Birth Outcomes in Los Angeles.
- Author
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Qi Meng, Liu, Jonathan, Jiaqi Shen, Del Rosario, Irish, Lakey, Pascale S. J., Shiraiwa, Manabu, Su, Jason, Weichenthal, Scott, Yifang Zhu, Oroumiyeh, Farzan, Paulson, Suzanne E., Jerrett, Michael, and Ritz, Beate
- Subjects
METAL analysis ,AIR pollution ,PARTICULATE matter ,PREMATURE infants ,CONFIDENCE intervals ,CARBON ,OXIDATIVE stress ,PRENATAL exposure delayed effects ,DESCRIPTIVE statistics ,REACTIVE oxygen species ,LOGISTIC regression analysis ,LONGITUDINAL method - Abstract
BACKGROUND: Although many studies have linked prenatal exposure to PM
2.5 to adverse birth outcomes, little is known about the effects of exposure to specific constituents of PM2.5 or mechanisms that contribute to these outcomes. OBJECTIVES: Our objective was to investigate effects of oxidative potential and PM2.5 metal components from non-exhaust traffic emissions, such as brake and tire wear, on the risk of preterm birth (PTB) and term low birth weight (TLBW). METHODS: For a birth cohort of 285,614 singletons born in Los Angeles County, California, in the period 2017–2019, we estimated speciated PM2.5 exposures modeled from land use regression with cokriging, including brake and tire wear related metals (barium and zinc), black carbon, and three markers of oxidative potential (OP), including modeled reactive oxygen species based on measured iron and copper (ROS), OH formation (OPOH ), and dithiothreitol (DTT) loss (OPDTT ). Using logistic regression, we estimated odds ratios (OR) and 95% confidence intervals (CI) for PTB and TLBW with speciated PM2.5 exposures and PM2.5 mass as continuous variables scaled by their interquartile range (IQR). RESULTS: For both metals and oxidative potential metrics, we estimated increased risks for PTB (ORs ranging from 1.01 to 1.03) and TLBW (ORs ranging from 1.02 to 1.05) per IQR exposure increment that were robust to adjustment for PM2.5 mass. Associations for PM2.5 mass, black carbon, metal components, and oxidative potential (especially ROS and OPOH ) with adverse birth outcomes were stronger in Hispanic, Black, and mixed-race or Native American women. DISCUSSION: Our results indicate that exposure to PM2.5 metals from brake and tire wear and particle components that contribute to oxidative potential were associated with an increased risk of PTB and TLBW in Los Angeles County, particularly among Hispanic, Black, and mixed-race or Native American women. Thus, reduction of PM2.5 mass only may not be sufficient to protect the most vulnerable pregnant women and children from adverse effects due to traffic source exposures. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
46. Effective mass accommodation for partitioning of organic compounds into surface films with different viscosities.
- Author
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Lakey, Pascale S. J., Cummings, Bryan E., Waring, Michael S., Morrison, Glenn C., and Shiraiwa, Manabu
- Abstract
Indoor surfaces can act as reservoirs and reaction media influencing the concentrations and type of species that people are exposed to indoors. Mass accommodation and partitioning are impacted by the phase state and viscosity of indoor surface films. We developed the kinetic multi-layer model KM-FILM to simulate organic film formation and growth, but it is computationally expensive to couple such comprehensive models with indoor air box models. Recently, a novel effective mass accommodation coefficient (α
eff ) was introduced for efficient and effective treatments of gas–particle partitioning. In this study, we extended this approach to a film geometry with αeff as a function of penetration depth into the film, partitioning coefficient, bulk diffusivity, and condensed-phase reaction rate constant. Comparisons between KM-FILM and the αeff method show excellent agreement under most conditions, but with deviations before the establishment of quasi-equilibrium within the penetration depth. We found that the deposition velocity of species and overall film growth are impacted by bulk diffusivity in highly viscous films (Db ∼<10−15 cm2 s−1 ). Reactions that lead to non-volatile products can increase film thicknesses significantly, with the extent of film growth being dependent on the gas-phase concentration, rate coefficient, partitioning coefficient and diffusivity. Amorphous semisolid films with Db > ∼10−17 –10−19 cm2 s−1 can be efficient SVOC reservoirs for compounds with higher partitioning coefficients as they can be released back to the gas phase over extended periods of time, while glassy solid films would not be able to act as reservoirs as gas-film partitioning is impeded. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
47. Quantifying the impact of relative humidity on human exposure to gas phase squalene ozonolysis products
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Lakey, Pascale S. J., primary, Zuend, Andreas, additional, Morrison, Glenn C., additional, Berkemeier, Thomas, additional, Wilson, Jake, additional, Arata, Caleb, additional, Goldstein, Allen H., additional, Wilson, Kevin R., additional, Wang, Nijing, additional, Williams, Jonathan, additional, Abbatt, Jonathan P. D., additional, and Shiraiwa, Manabu, additional
- Published
- 2023
- Full Text
- View/download PDF
48. Iodine emission from the reactive uptake of ozone to simulated seawater
- Author
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Schneider, Stephanie R., primary, Lakey, Pascale S. J., additional, Shiraiwa, Manabu, additional, and Abbatt, Jonathan P. D., additional
- Published
- 2023
- Full Text
- View/download PDF
49. Correction: Iodine emission from the reactive uptake of ozone to simulated seawater
- Author
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Schneider, Stephanie R., primary, Lakey, Pascale S. J., additional, Shiraiwa, Manabu, additional, and Abbatt, Jonathan P. D., additional
- Published
- 2023
- Full Text
- View/download PDF
50. Partitioning of reactive oxygen species from indoor surfaces to indoor aerosols
- Author
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Glenn C. Morrison, Azin Eftekhari, Pascale S. J. Lakey, Manabu Shiraiwa, Bryan E. Cummings, Michael S. Waring, and Brent Williams
- Subjects
Aerosols ,Air Pollutants ,Ozone ,Air Pollution, Indoor ,Public Health, Environmental and Occupational Health ,Environmental Chemistry ,Particulate Matter ,General Medicine ,Particle Size ,Management, Monitoring, Policy and Law ,Reactive Oxygen Species ,Environmental Monitoring - Abstract
Reactive oxygen species (ROS) are among the species thought to be responsible for the adverse health effects of particulate matter (PM) inhalation. Field studies suggest that indoor sources of ROS contribute to measured ROS on PM in indoor air. We hypothesize that ozone reacts on indoor surfaces to form semi-volatile ROS, in particular organic peroxides (OPX), which partition to airborne particles. To test this hypothesis, we modeled ozone-induced formation of OPX, its decay and its partitioning to PM in a residential building and compared the results to field measurements. Simulations indicate that, while ROS of outdoor origin is the primary contributor to indoor ROS (in PM), a substantial fraction of ROS present in indoor PM is from ozone-surface chemistry. At an air change rate equal to 1/h, and an outdoor ozone mixing ratio of 35 ppb, 25% of the ROS concentration in air is due to indoor formation and partitioning of OPX to PM. For the same conditions, but with a modest indoor source of PM (1.5 mg h
- Published
- 2022
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