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3 results on '"Pascal Barantin"'

1. Genomic Instability in Somatic Hybridization between Poncirus and Citrus Species Aiming to Create New Rootstocks

Author

Dominique Dambier, Pascal Barantin, Gabriel Boulard, Gilles Costantino, Pierre Mournet, Aude Perdereau, Raphaël Morillon, and Patrick Ollitrault

Subjects
protoplast fusion, somatic embryogenesis, genotyping by sequencing, somaclonal variation, Citrus sinensis, Citrus reticulata, Agriculture (General), S1-972
Abstract

Rootstocks are an important component for citrus adaptation to increasing biotic and abiotic stresses resulting from global climate change. There is a strong complementarity between Citrus species, which adapt to abiotic stresses, and Poncirus trifoliata and its intergeneric hybrids, which exhibit resistances or tolerances to major diseases and pests. Thus, symmetrical somatic hybridization between complementary diploid rootstocks of these two genera appears to be an efficient way to develop new tetraploid rootstocks in order to address the new challenges of the citrus industry. New intergeneric somatic hybrids were obtained by electrofusion between protoplasts of Citrus and P. trifoliata hybrids. Extensive characterization of the nuclear and cytoplasmic genomes was performed by genotyping-by-sequencing (GBS) analysis. This revealed diploid cybrids and nuclear somatic hybrids. Mitochondrial genomes were mostly inherited from the callus parent, but homologous recombination events were observed for one parental combination. Chloroplasts exhibited random uniparental inheritance. GBS revealed local chromosomal instabilities for all nuclear somatic hybrids and whole chromosome eliminations for two hybrids. However, at the whole genome level, symmetrical addition of the nuclear genomes of both parents was predominant and all somatic hybrids displayed at least one trifoliate orange haplotype throughout the genome.

Published
2022
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2. Production and molecular characterization of new citrus hybrids using somatic hybridization coupled with nuclear and chloroplastic microsatellite markers

Author

Ouiam Chetto, Patrick Ollitrault, Hamid Benyahia, Pascal Barantin, and Dominique Dambier

Subjects
Citrus hybrids, education.field_of_study, biology, Citrus macrophylla, Population, fungi, food and beverages, Horticulture, Protoplast, biology.organism_classification, Chloroplast DNA, Botany, Plant breeding, Ploidy, education, Hybrid
Abstract

Somatic hybridization is a powerful biotechnology technique which helps to create new somatic genotypes combining desirable traits from both parents. Nowadays, its use has become an integral part of plant breeding programs. In this study, plants derived from five protoplast fusion combinations, i.e. “Citrus macrophylla + Valencia orange (C. sinensis)”, “C. macrophylla + Kinnow mandarin (C. nobilis × C. deliciosa)”, “Carrizo citrange (C. sinensis × P. trifoliata) + Willow leaf mandarin (C. deliciosa)”, “Volkamer lemon (C. volkameriana) + Willow leaf mandarin” and “Nasnaran mandarin (C. amblycarpa) + Willow leaf mandarin” were subjected to molecular analysis of nuclear and cytoplasmic genome. The use of SSR markers confirmed the formation of hybrids and somatic cybrids within this population. Flow cytometry analysis has further shown that 12 of the 14 regenerated plants were diploid, while two, which originated from the C. macrophylla + Valencia orange combination, were tetraploid. The analysis of cytoplasmic genome using universal primers revealed that chloroplast DNA (cpDNA) of these genotypes was inherited randomly, while their mitochondrial genomes were inherited from the embryonic parents.

Published
2019

3. NEW CYBRIDS RESULTING FROM ASEXUAL PATHWAY: A PROMISE OF CYBRIDIZATION FOR CREATING NEW CITRUS ROOTSTOCKS AND CULTIVARS

Author

Fanny Petit, Pascal Barantin, Dominique Dambier, and Patrick Ollitrault

Subjects
Genetics, Sterility, fungi, food and beverages, Cultivar, biochemical phenomena, metabolism, and nutrition, Horticulture, Protoplast, Biology, Rootstock, Genetic recombination, Cytoplast
Abstract

Cybridization should be a way to induce phenotypical variations in highly heterozygous secondary citrus species or genotypes, without breaking their very complex multilocus structure by sexual recombination. It should have particular interest to manipulate the nucleo-cytoplasmic male sterility, to transfer tolerance traits with cytoplasmic determinisms and eventually to improve tolerance to oxidative stress. The present work is focused on the creation of new cybrids through three different approaches of protoplast fusion: (i) symmetric hybridization, (ii) donor-recipient protoplast fusion by UV irradiation of donor protoplasts, and (iii) cytoplasts + protoplast fusion. Sixteen combinations of symmetric protoplast fusion have produced cybrids. No cybrid was obtained after UV irradiation of donor protoplasts while three combinations of cytoplasts + protoplast fusion produced cybrids. Therefore, cytoplast fusion appears much more promising than the UV approach for targeted cybridization. The innovative plant material is currently propagated for further cultivar and rootstock selection and for basic researches on the impact of nucleo-cytoplasmic interaction on desirable traits. (Resume d'auteur)

Published
2015
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