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1. Seroprevalence of SARS-CoV-2 antibodies among healthcare workers in Dutch hospitals after the 2020 first wave: a multicentre cross-sectional study with prospective follow-up

Author

Recanatini, Claudia, GeurtsvanKessel, Corine H., Pas, Suzan D., Broens, Els M., Maas, Martje, van Mansfeld, Rosa, Mutsaers-van Oudheusden, Anne J. G., van Rijen, Miranda, Schippers, Emile F., Stegeman, Arjan, Tami, Adriana, Veldkamp, Karin Ellen, Visser, Hannah, Voss, Andreas, Wegdam-Blans, Marjolijn C. A., Wertheim, Heiman F. L., Wever, Peter C., Koopmans, Marion P. G., Kluytmans, Jan A. J. W., and Kluytmans-van den Bergh, Marjolein F. Q.

Published
2023
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2. Head-to-head comparison of the accuracy of saliva and nasal rapid antigen SARS-CoV-2 self-testing: cross-sectional study

Author

Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Stohr, Joep J. J. M., Lodder, Esther B., Hellwich, Marloes, Molenkamp, Richard, Igloi, Zsofia, Wijers, Constantijn, Vroom, Irene H., Nagel-Imming, Carla R. S., Han, Wanda G. H., Kluytmans, Jan A. J. W., van den Hof, Susan, van de Wijgert, Janneke H. H. M., and Moons, Karel G. M.

Published
2022
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3. Detection of SARS-CoV-2 infection in the general population by three prevailing rapid antigen tests: cross-sectional diagnostic accuracy study

Author

Venekamp, Roderick P., Veldhuijzen, Irene K., Moons, Karel G. M., van den Bijllaardt, Wouter, Pas, Suzan D., Lodder, Esther B., Molenkamp, Richard, Igloi, Zsofi, Wijers, Constantijn, dos Santos, Claudy Oliveira, Debast, Sylvia B., Bruins, Marjan J., Polad, Khaled, Nagel-Imming, Carla R. S., Han, Wanda G. H., van de Wijgert, Janneke H. H. M., van den Hof, Susan, and Schuit, Ewoud

Published
2022
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4. Self-testing for the detection of SARS-CoV-2 infection with rapid antigen tests for people with suspected COVID-19 in the community

Author

Stohr, Joep J.J.M., Zwart, Vivian F., Goderski, Gabriel, Meijer, Adam, Nagel-Imming, Carla R.S., Kluytmans-van den Bergh, Marjolein F.Q., Pas, Suzan D., van den Oetelaar, Femke, Hellwich, Marloes, Gan, Kim H., Rietveld, Ariene, Verweij, Jaco J., Murk, Jean-Luc, van den Bijllaardt, Wouter, and Kluytmans, Jan A.J.W.

Published
2022
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6. Virus genomes reveal factors that spread and sustained the Ebola epidemic

Author

Dudas, Gytis, Carvalho, Luiz Max, Bedford, Trevor, Tatem, Andrew J, Baele, Guy, Faria, Nuno R, Park, Daniel J, Ladner, Jason T, Arias, Armando, Asogun, Danny, Bielejec, Filip, Caddy, Sarah L, Cotten, Matthew, D’Ambrozio, Jonathan, Dellicour, Simon, Di Caro, Antonino, Diclaro, Joseph W, Duraffour, Sophie, Elmore, Michael J, Fakoli, Lawrence S, Faye, Ousmane, Gilbert, Merle L, Gevao, Sahr M, Gire, Stephen, Gladden-Young, Adrianne, Gnirke, Andreas, Goba, Augustine, Grant, Donald S, Haagmans, Bart L, Hiscox, Julian A, Jah, Umaru, Kugelman, Jeffrey R, Liu, Di, Lu, Jia, Malboeuf, Christine M, Mate, Suzanne, Matthews, David A, Matranga, Christian B, Meredith, Luke W, Qu, James, Quick, Joshua, Pas, Suzan D, Phan, My VT, Pollakis, Georgios, Reusken, Chantal B, Sanchez-Lockhart, Mariano, Schaffner, Stephen F, Schieffelin, John S, Sealfon, Rachel S, Simon-Loriere, Etienne, Smits, Saskia L, Stoecker, Kilian, Thorne, Lucy, Tobin, Ekaete Alice, Vandi, Mohamed A, Watson, Simon J, West, Kendra, Whitmer, Shannon, Wiley, Michael R, Winnicki, Sarah M, Wohl, Shirlee, Wölfel, Roman, Yozwiak, Nathan L, Andersen, Kristian G, Blyden, Sylvia O, Bolay, Fatorma, Carroll, Miles W, Dahn, Bernice, Diallo, Boubacar, Formenty, Pierre, Fraser, Christophe, Gao, George F, Garry, Robert F, Goodfellow, Ian, Günther, Stephan, Happi, Christian T, Holmes, Edward C, Kargbo, Brima, Keïta, Sakoba, Kellam, Paul, Koopmans, Marion PG, Kuhn, Jens H, Loman, Nicholas J, Magassouba, N’Faly, Naidoo, Dhamari, Nichol, Stuart T, Nyenswah, Tolbert, Palacios, Gustavo, Pybus, Oliver G, Sabeti, Pardis C, Sall, Amadou, Ströher, Ute, Wurie, Isatta, Suchard, Marc A, Lemey, Philippe, and Rambaut, Andrew

Subjects
Infectious Diseases, Emerging Infectious Diseases, Biodefense, Vaccine Related, Prevention, Infection, Good Health and Well Being, Climate, Disease Outbreaks, Ebolavirus, Genome, Viral, Geography, Hemorrhagic Fever, Ebola, Humans, Internationality, Linear Models, Molecular Epidemiology, Phylogeny, Travel, General Science & Technology
Abstract

The 2013-2016 West African epidemic caused by the Ebola virus was of unprecedented magnitude, duration and impact. Here we reconstruct the dispersal, proliferation and decline of Ebola virus throughout the region by analysing 1,610 Ebola virus genomes, which represent over 5% of the known cases. We test the association of geography, climate and demography with viral movement among administrative regions, inferring a classic 'gravity' model, with intense dispersal between larger and closer populations. Despite attenuation of international dispersal after border closures, cross-border transmission had already sown the seeds for an international epidemic, rendering these measures ineffective at curbing the epidemic. We address why the epidemic did not spread into neighbouring countries, showing that these countries were susceptible to substantial outbreaks but at lower risk of introductions. Finally, we reveal that this large epidemic was a heterogeneous and spatially dissociated collection of transmission clusters of varying size, duration and connectivity. These insights will help to inform interventions in future epidemics.

Published
2017

8. COVID-19 in health-care workers in three hospitals in the south of the Netherlands: a cross-sectional study

Author

Sikkema, Reina S, Pas, Suzan D, Nieuwenhuijse, David F, O'Toole, Áine, Verweij, Jaco, van der Linden, Anne, Chestakova, Irina, Schapendonk, Claudia, Pronk, Mark, Lexmond, Pascal, Bestebroer, Theo, Overmars, Ronald J, van Nieuwkoop, Stefan, van den Bijllaardt, Wouter, Bentvelsen, Robbert G, van Rijen, Miranda M L, Buiting, Anton G M, van Oudheusden, Anne J G, Diederen, Bram M, Bergmans, Anneke M C, van der Eijk, Annemiek, Molenkamp, Richard, Rambaut, Andrew, Timen, Aura, Kluytmans, Jan A J W, Oude Munnink, Bas B, Kluytmans van den Bergh, Marjolein F Q, and Koopmans, Marion P G

Published
2020
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11. Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Author

Dumke, Roger, Benitez, Alvaro J., Chalker, Victoria, Gullsby, Karolina, Henrich, Birgit, Hidalgo-Grass, Carlos, Hoogenboezem, Theo, Kese, Darja, Loens, Katherine, Maaskant, Jolanda, Michael-Gayego, Ayelet, Moses, Allon E., Nir-Paz, Ran, Pas, Suzan D., Pereyre, Sabine, Petersen, Randi F., Rosenblatt, Meike, van Rossum, Annemarie M.C., Uldum, Søren A., Unger, Wendy W.J., Ursi, Dominique, Winchell, Jonas M., and Bebear, Cecile

Published
2017
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14. Diagnostic accuracy of SARS-CoV-2 rapid antigen self-tests in asymptomatic individuals in the omicron period: a cross-sectional study

Author

Venekamp, Roderick P., primary, Schuit, Ewoud, additional, Hooft, Lotty, additional, Veldhuijzen, Irene K., additional, van den Bijllaardt, Wouter, additional, Pas, Suzan D., additional, Zwart, Vivian F., additional, Lodder, Esther B., additional, Hellwich, Marloes, additional, Koppelman, Marco, additional, Molenkamp, Richard, additional, Wijers, Constantijn J.H., additional, Vroom, Irene H., additional, Smeets, Leonard C., additional, Nagel-Imming, Carla R.S., additional, Han, Wanda G.H., additional, van den Hof, Susan, additional, Kluytmans, Jan A.J.W., additional, van de Wijgert, Janneke H.H.M., additional, and Moons, Karel G.M., additional

Published
2023
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15. Detection of SARS-CoV-2 in Air and on Surfaces in Rooms of Infected Nursing Home Residents

Author

IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, Linde, Kimberly J, Wouters, Inge M, Kluytmans, Jan A J W, Kluytmans-van den Bergh, Marjolein F Q, Pas, Suzan D, Koopmans, Marion P G, GeurtsvanKessel, Corine H, Meier, Melanie, Meijer, Patrick, Raben, Ceder R, Spithoven, Jack, Tersteeg-Zijderveld, Monique H G, COCON Consortium, Heederik, Dick J J, Dohmen, Wietske, IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, Linde, Kimberly J, Wouters, Inge M, Kluytmans, Jan A J W, Kluytmans-van den Bergh, Marjolein F Q, Pas, Suzan D, Koopmans, Marion P G, GeurtsvanKessel, Corine H, Meier, Melanie, Meijer, Patrick, Raben, Ceder R, Spithoven, Jack, Tersteeg-Zijderveld, Monique H G, COCON Consortium, Heederik, Dick J J, and Dohmen, Wietske

Published
2023

16. Elevated risk of infection with SARS-CoV-2 Beta, Gamma, and Delta variants compared with Alpha variant in vaccinated individuals

Author

Andeweg, Stijn P., Vennema, Harry, Veldhuijzen, Irene, Smorenburg, Naomi, Schmitz, Dennis, Zwagemaker, Florian, Van Gageldonk-Lafeber, Arianne B., Hahne, Susan J.M., Reusken, Chantal, Knol, Mirjam J., Eggink, Dirk, Schinkel, Janke, Welkers, Matthijs R.A., Jonges, Marcel, Koopsen, Jelle, Jong, Menno D.de, Molenkamp, Richard, Nieuwenhuijse, David F., Sikkema, Reina S., Munnink, Bas B.Oude, Koopmans, Marion, van der Zanden, Adri, Manrho, Laura, Beer, Jessica de, Boers, Stefan A., Meijers, Erin, Vreeswijk, Tom, Sidorov, Igor A., Dunk, Djoo, Smit, Pieter W., Pas, Suzan D., Verweij, Jaco J., Stohr, Joep J.J.M., Dingemans, Jozef, Veer, Brian van der, Alphen, Lieke van, Savelkoul, Paul, Niesters, Hubert G.M., Forero, Erley F.Lizarazo, Fliss, Monika A., Gard, Lilli, Tanja, Anniek A.N., Schuurman10, Rob, Wensing, Annemarie M.J., Hofstra, L. Marije, Wertheim, Heiman F.L., Faber, Timor, Kroneman, Annelies, Mulder, Maarten, Schipper, Maarten, Andeweg, Stijn P., Vennema, Harry, Veldhuijzen, Irene, Smorenburg, Naomi, Schmitz, Dennis, Zwagemaker, Florian, Van Gageldonk-Lafeber, Arianne B., Hahne, Susan J.M., Reusken, Chantal, Knol, Mirjam J., Eggink, Dirk, Schinkel, Janke, Welkers, Matthijs R.A., Jonges, Marcel, Koopsen, Jelle, Jong, Menno D.de, Molenkamp, Richard, Nieuwenhuijse, David F., Sikkema, Reina S., Munnink, Bas B.Oude, Koopmans, Marion, van der Zanden, Adri, Manrho, Laura, Beer, Jessica de, Boers, Stefan A., Meijers, Erin, Vreeswijk, Tom, Sidorov, Igor A., Dunk, Djoo, Smit, Pieter W., Pas, Suzan D., Verweij, Jaco J., Stohr, Joep J.J.M., Dingemans, Jozef, Veer, Brian van der, Alphen, Lieke van, Savelkoul, Paul, Niesters, Hubert G.M., Forero, Erley F.Lizarazo, Fliss, Monika A., Gard, Lilli, Tanja, Anniek A.N., Schuurman10, Rob, Wensing, Annemarie M.J., Hofstra, L. Marije, Wertheim, Heiman F.L., Faber, Timor, Kroneman, Annelies, Mulder, Maarten, and Schipper, Maarten

Abstract

The extent to which severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) break through infection- or vaccine-induced immunity is not well understood. We analyzed 28,578 sequenced SARS-CoV-2 samples from individuals with known immune status obtained through national community testing in the Netherlands from March to August 2021. We found evidence of an increased risk of infection by the Beta (B.1.351), Gamma (P.1), or Delta (B.1.617.2) variants compared with the Alpha (B.1.1.7) variant after vaccination. No clear differences were found between vaccines. However, the effect was larger in the first 14 to 59 days after complete vaccination compared with ≥60 days. In contrast to vaccine-induced immunity, there was no increased risk for reinfection with Beta, Gamma, or Delta variants relative to the Alpha variant in individuals with infection-induced immunity.

Published
2023

17. Diagnostic accuracy of SARS-CoV-2 rapid antigen self-tests in asymptomatic individuals in the omicron period:a cross-sectional study

Author

Venekamp, Roderick P., Schuit, Ewoud, Hooft, Lotty, Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Zwart, Vivian F., Lodder, Esther B., Hellwich, Marloes, Koppelman, Marco, Molenkamp, Richard, Wijers, Constantijn J.H., Vroom, Irene H., Smeets, Leonard C., Nagel-Imming, Carla R.S., Han, Wanda G.H., van den Hof, Susan, Kluytmans, Jan A.J.W., van de Wijgert, Janneke H.H.M., Moons, Karel G.M., Venekamp, Roderick P., Schuit, Ewoud, Hooft, Lotty, Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Zwart, Vivian F., Lodder, Esther B., Hellwich, Marloes, Koppelman, Marco, Molenkamp, Richard, Wijers, Constantijn J.H., Vroom, Irene H., Smeets, Leonard C., Nagel-Imming, Carla R.S., Han, Wanda G.H., van den Hof, Susan, Kluytmans, Jan A.J.W., van de Wijgert, Janneke H.H.M., and Moons, Karel G.M.

Abstract

Objectives: To assess the performances of three commonly used antigen rapid diagnostic tests used as self-tests in asymptomatic individuals in the Omicron period. Methods: We performed a cross-sectional diagnostic test accuracy study in the Omicron period in three public health service COVID-19 test sites in the Netherlands, including 3600 asymptomatic individuals aged ≥ 16 years presenting for SARS-CoV-2 testing for any reason except confirmatory testing after a positive self-test. Participants were sampled for RT-PCR (reference test) and received one self-test (either Acon Flowflex [Flowflex], MP Biomedicals (MPBio), or Siemens-Healthineers CLINITEST [CLINITEST]) to perform unsupervised at home. Diagnostic accuracies of each self-test were calculated. Results: Overall sensitivities were 27.5% (95% CI, 21.3–34.3%) for Flowflex, 20.9% (13.9–29.4%) for MPBio, and 25.6% (19.1–33.1%) for CLINITEST. After applying a viral load cut-off (≥5.2 log10 SARS-CoV-2 E-gene copies/mL), sensitivities increased to 48.3% (37.6–59.2%), 37.8% (22.5–55.2%), and 40.0% (29.5–51.2%), respectively. Specificities were >99% for all tests in most analyses. Discussion: The sensitivities of three commonly used SARS-CoV-2 antigen rapid diagnostic tests when used as self-tests in asymptomatic individuals in the Omicron period were very low. Antigen rapid diagnostic test self-testing in asymptomatic individuals may only detect a minority of infections at that point in time. Repeated self-testing in case of a negative self-test is advocated to improve the diagnostic yield, and individuals should be advised to re-test when symptoms develop.

Published
2023

18. Diagnostic accuracy of SARS-CoV-2 rapid antigen self-tests in asymptomatic individuals in the Omicron period: a cross-sectional study

Author

HAG Onderzoek, Child Health, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Epi Methoden Team 4, JC onderzoeksprogramma Methodologie, Epidemiology & Health Economics, Medische Microbiologie, Epi Infectieziekten Team 1b, Epi Methoden, Cancer, Venekamp, Roderick P, Schuit, Ewoud, Hooft, Lotty, Veldhuijzen, Irene K, van den Bijllaardt, Wouter, Pas, Suzan D, Zwart, Vivian F, Lodder, Esther B, Hellwich, Marloes, Koppelman, Marco, Molenkamp, Richard, Wijers, Constantijn Jh, Vroom, Irene H, Smeets, Leonard C, Nagel-Imming, Carla R S, Han, Wanda G H, van den Hof, Susan, Kluytmans, Jan AJW, van de Wijgert, Janneke H M, Moons, Karel G M, HAG Onderzoek, Child Health, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Epi Methoden Team 4, JC onderzoeksprogramma Methodologie, Epidemiology & Health Economics, Medische Microbiologie, Epi Infectieziekten Team 1b, Epi Methoden, Cancer, Venekamp, Roderick P, Schuit, Ewoud, Hooft, Lotty, Veldhuijzen, Irene K, van den Bijllaardt, Wouter, Pas, Suzan D, Zwart, Vivian F, Lodder, Esther B, Hellwich, Marloes, Koppelman, Marco, Molenkamp, Richard, Wijers, Constantijn Jh, Vroom, Irene H, Smeets, Leonard C, Nagel-Imming, Carla R S, Han, Wanda G H, van den Hof, Susan, Kluytmans, Jan AJW, van de Wijgert, Janneke H M, and Moons, Karel G M

Published
2023

19. Whole-Blood Testing for Diagnosis of Acute Zika Virus Infections in Routine Diagnostic Setting

Author

Voermans, Jolanda J.C., Pas, Suzan D., van der Linden, Anne, GeurtsvanKessel, Corine, Koopmans, Marion, van der Eijk, Annemiek, and Reusken, Chantal B.E.M.

Subjects
Medical tests -- Analysis, Birth defects -- Diagnosis, Disease transmission -- Diagnosis, Zika virus -- Analysis, Infection -- Diagnosis, EDTA -- Analysis, Pregnant women -- Analysis, RNA -- Analysis, Health
Abstract

Since its emergence in South and Central America in 2015/2016, Zika virus (genus Flavivirus) has become a major public health concern. Zika virus infections are linked to congenital malformations in [...]

Published
2019
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20. Detection of SARS-CoV-2 in Air and on Surfaces in Rooms of Infected Nursing Home Residents

Author

Linde, Kimberly J, Wouters, Inge M, Kluytmans, Jan A J W, Kluytmans-van den Bergh, Marjolein F Q, Pas, Suzan D, Koopmans, Marion P G, GeurtsvanKessel, Corine H, Meier, Melanie, Meijer, Patrick, Raben, Ceder R, Spithoven, Jack, Tersteeg-Zijderveld, Monique H G, Heederik, Dick J J, Dohmen, Wietske, IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, and Virology

Subjects
nursing home, SARS-CoV-2, SDG 3 - Good Health and Well-being, Public Health, Environmental and Occupational Health, surface, air levels
Abstract

There is an ongoing debate on airborne transmission of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) as a risk factor for infection. In this study, the level of SARS-CoV-2 in air and on surfaces of SARS-CoV-2 infected nursing home residents was assessed to gain insight in potential transmission routes. During outbreaks, air samples were collected using three different active and one passive air sampling technique in rooms of infected patients. Oropharyngeal swabs (OPS) of the residents and dry surface swabs were collected. Additionally, longitudinal passive air samples were collected during a period of 4 months in common areas of the wards. Presence of SARS-CoV-2 RNA was determined using RT-qPCR, targeting the RdRp- and E-genes. OPS, samples of two active air samplers and surface swabs with Ct-value ≤35 were tested for the presence of infectious virus by cell culture. In total, 360 air and 319 surface samples from patient rooms and common areas were collected. In rooms of 10 residents with detected SARS-CoV-2 RNA in OPS, SARS-CoV-2 RNA was detected in 93 of 184 collected environmental samples (50.5%) (lowest Ct 29.5), substantially more than in the rooms of residents with negative OPS on the day of environmental sampling (n = 2) (3.6%). SARS-CoV-2 RNA was most frequently present in the larger particle size fractions [>4 μm 60% (6/10); 1–4 μm 50% (5/10)

Published
2023

21. Assay optimization for molecular detection of Zika virus/ Optimisation des tests pour la detection moleculaire du virus Zika/ Optimizacion de la prueba para la deteccion molecular del virus de Zika

Author

Corman, Victor M., Rasche, Andrea, Baronti, Cecile, Aldabbagh, Souhaib, Cadar, Daniel, Reusken, Chantal BEM, Pas, Suzan D., Goorhuis, Abraham, Schinkel, Janke, Molenkamp, Richard, Kiimmerer, Beate M., Bleicker, Tobias, Brunink, Sebastian, Eschbach-Bludau, Monika, Eis-Hubinger, Anna M., Koopmans, Marion P., Schmidt-Chanasit, Jonas, Grobusch, Martin P., de Lamballerie, Xavier, Drosten, Christian, and Drexler, Jan Felix

Subjects
Biological assay -- Innovations, Genotype -- Identification and classification, Molecular diagnostic techniques -- Innovations, Health
Abstract

Objective To examine the diagnostic performance of real-time reverse transcription (RT)-polymerase chain reaction (PCR) assays for Zika virus detection. Methods We compared seven published real-time RT-PCR assays and two new assays that we have developed. To determine the analytical sensitivity of each assay, we constructed a synthetic universal control ribonucleic acid (uncRNA) containing all of the assays' target regions on one RNA strand and spiked human blood or urine with known quantities of African or Asian Zika virus strains. Viral loads in 33 samples from Zika virus-infected patients were determined by using one of the new assays. Findings Oligonucleotides of the published real-time RT-PCR assays, showed up to 10 potential mismatches with the Asian lineage causing the current outbreak, compared with 0 to 4 mismatches for the new assays. The 95% lower detection limit of the seven most sensitive assays ranged from 2.1 to 12.1 uncRNA copies/reaction. Two assays had lower sensitivities of 17.0 and 1373.3 uncRNA copies/reaction and showed a similar sensitivity when using spiked samples. The mean viral loads in samples from Zika virus-infected patients were 5 x 104 RNA copies/mL of blood and 2 x [10.sup.4] RNA coples/mL of urine. Conclusion We provide reagents and updated protocols for Zika virus detection suitable for the current outbreak strains. Some published assays might be unsuitable for Zika virus detection, due to the limited sensitivity and potential incompatibility with some strains. Viral concentrations in the clinical samples were close to the technical detection limit, suggesting that the use of insensitive assays will cause false-negative results. Objectif Etudier la performance diagnostique des tests bases sur l'amplification en chaine par polymerase (PCR) en temps reel apres transcription inverse (RT) pour detecter le virus Zika. Methodes Nous avons compare sept tests publies utilisant la RT-PCR en temps reel et deux nouveaux tests developpes par nos soins. Afin de determiner la sensibilite analytique de chaque test, nous avons concu un acide ribonucleique synthetique de controle universel (ARNcun) contenant toutes les regions ciblees par les tests sur une helice ARN et enrichi de l'urine ou du sang humain de quantites connues de souches africaines ou asiatiques du virus Zika. Les charges virales dans 33 echantillons provenant de patients infectes par le virus Zika ont ete determinees a l'aide de l'un des nouveaux tests. Resultats Les oligonucleotides des tests publies utilisant la RT-PCR en temps reel ont presente jusqu'a TOmauvais apparlements potentiels avec la lignee asiatique provoquant l'epidemie actuelle, alors qu'on a constate de 0 a 4 mauvais appariements dans le cas des nouveaux tests. La limite de detection inferieure a 95% des sept tests les plus sensibles variait de 2,1 a 12,1 copies dARNcun/reaction. Deux tests presentaient des sensibilites plus basses de 17,0 et 1373,3 copies d'ARNcun/reaction et montraient une sensibilite similaire lorsque des echantillons enrichis etaient utilises. Les charges virales moyennes dans les echantillons provenant de patients infectes par le virus Zika etaient de 5 x 104 copies d'ARN/mL de sang et de 2 x 104 copies dARN/mL d'urine. Conclusion Nous proposons pour detecter le virus Zika des reactifs et des protocoles actualises, adaptes aux souches responsables de la flambee actuelle.Tous les tests publies ne permettent pas de detecter le virus Zika en raison d'une sensibilite limitee et d'une incompatibilite potentielle avec certaines souches. Les concentrations virales dans les echantillons cliniques etaient proches de la limite de detection technique, ce qui laisse penser que l'utilisation de tests insensibles donnera des resultats faussement negatifs. Objetivo Examinar el rendimiento del diagnostico de las pruebas de reaccion en cadena de la polimerasa de transcriptasa inversa (RT-PCR) en tiempo real para la deteccion del virus de Zika. Metodos Se compararon siete pruebas de RT-PCR en tiempo real publicadas y dos pruebas nuevas que se han desarrollado. Con el fin de determinar la sensibilidad analitica de cada prueba, se construyo un acido ribonucleico sintetico de control universal (uncRNA) que contenia todas las regiones objetivo de las pruebas en una hebra de RNA y se anadio a sangre u orina humana con cantidades conocidas de cepas de virus de Zika de Asia o Africa. Se determinaron las cargas viricas en 33 muestras procedentes de pacientes infectados por el virus de Zika a traves de una de las pruebas nuevas. Resultados Los oligonucleotidos de las pruebas de RT-PCR en tiempo real publicadas mostraron hasta 10 posibles discordancias con el linaje de Asia causante de los brotes actuales, en comparacion con 0 de 4 discordancias en el caso de las pruebas nuevas. El limite de deteccion inferior del 95% de las siete pruebas mas sensibles abarco de 2,1 a 12,1 copias/reaccion de uncRNA. Dos pruebas mostraron sensibilidades inferiores de 17,0 y 1373,3 copias/reaccion de uncRNA y presentaron una sensibilidad similar al usar muestras Infectadas. Las cargas viricas medias en las muestras procedentes de pacientes infectados por el virus de Zika fueron de 5 x 104 copias de RNA/mL de sangre y de 2 x [10.sup.4] coplas de RNA/ml de orina. Conclusion Se proporcionan reactivos y protocolos actualizados para la deteccion adecuada del virus de Zika en el caso de las cepas de brotes actuales. Algunas pruebas publicadas pueden no ser adecuadas para la deteccion del virus de Zika, debido a la limitada sensibilidad y a la posible incompatibilidad con algunas cepas. Las concentraciones viricas en las muestras clinicas se acercaron al limite de deteccion tecnico, lo que sugeria que el uso de pruebas Intensivas causaria resultados falsos negativos., Introduction The Zika virus is a mosquito-borne flavivirus with an approximately 11 kilobase ribonucleic acid (RNA) genome. (1) The virus usually causes a mild infection in adults, symptoms include fever, [...]

Published
2016
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22. SARS-CoV-2 Alpha-Variant Outbreak Amongst a Partially Vaccinated Long-Term Care Facility Population in The Netherlands—Phylogenetic Analysis and Infection Control Observations

Author

Van der Moeren, Nathalie, primary, Weterings, Veronica A. T. C., additional, Pas, Suzan D., additional, Verweij, Jaco J., additional, van den Bijllaardt, Wouter, additional, Geurts, Joyce, additional, Zwart, Vivian F., additional, Lodder, Esther B., additional, Kluytmans, Jan A. J. W., additional, Murk, Jean-Luc, additional, and Stohr, Joep J. J. M., additional

Published
2022
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23. Diagnostic accuracy of covid-19 rapid antigen tests with unsupervised self-sampling in people with symptoms in the omicron period: cross sectional study

Author

Schuit, Ewoud, primary, Venekamp, Roderick P, additional, Hooft, Lotty, additional, Veldhuijzen, Irene K, additional, van den Bijllaardt, Wouter, additional, Pas, Suzan D, additional, Zwart, Vivian F, additional, Lodder, Esther B, additional, Hellwich, Marloes, additional, Koppelman, Marco, additional, Molenkamp, Richard, additional, Wijers, Constantijn J H, additional, Vroom, Irene H, additional, Smeets, Leonard C, additional, Nagel-Imming, Carla R S, additional, Han, Wanda G H, additional, van den Hof, Susan, additional, Kluytmans, Jan A J W, additional, van de Wijgert, Janneke H H M, additional, and Moons, Karel G M, additional

Published
2022
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24. Immune-escape mutations and stop-codons in HBsAg develop in a large proportion of patients with chronic HBV infection exposed to anti-HBV drugs in Europe

Author

Colagrossi, Luna, Hermans, Lucas E., Salpini, Romina, Di Carlo, Domenico, Pas, Suzan D., Alvarez, Marta, Ben-Ari, Ziv, Boland, Greet, Bruzzone, Bianca, Coppola, Nicola, Seguin-Devaux, Carole, Dyda, Tomasz, Garcia, Federico, Kaiser, Rolf, Köse, Sukran, Krarup, Henrik, Lazarevic, Ivana, Lunar, Maja M., Maylin, Sarah, Micheli, Valeria, Mor, Orna, Paraschiv, Simona, Paraskevis, Dimitros, Poljak, Mario, Puchhammer-Stöckl, Elisabeth, Simon, François, Stanojevic, Maja, Stene-Johansen, Kathrine, Tihic, Nijaz, Trimoulet, Pascale, Verheyen, Jens, Vince, Adriana, Lepej, Snjezana Zidovec, Weis, Nina, Yalcinkaya, Tülay, Boucher, Charles A. B., Wensing, Annemarie M. J., Perno, Carlo F., Svicher, Valentina, and on behalf of the HEPVIR working group of the European Society for translational antiviral research (ESAR)

Published
2018
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25. Diagnostic accuracy of SARS-CoV-2 rapid antigen self-tests in asymptomatic individuals in the Omicron period: cross sectional study

Author

Venekamp, Roderick P, primary, Schuit, Ewoud, additional, Hooft, Lotty, additional, Veldhuijzen, Irene K, additional, Bijllaardt, Wouter, additional, Pas, Suzan D, additional, Zwart, Vivian F, additional, Lodder, Esther B, additional, Hellwich, Marloes, additional, Koppelman, Marco, additional, Molenkamp, Richard, additional, Wijers, Constantijn, additional, Vroom, Irene H, additional, Smeets, Leonard C, additional, Nagel-Imming, Carla RS, additional, Han, Wanda GH, additional, van den Hof, Susan, additional, Kluytmans, Jan AJ, additional, van de Wijgert, Janneke HHM, additional, and Moons, Karel GM, additional

Published
2022
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27. September through October 2010 multi-centre study in the Netherlands examining laboratory ability to detect enterovirus 68, an emerging respiratory pathogen

Author

Jaramillo-Gutierrez, Giovanna, Benschop, Kimberley S.M., Claas, Eric C.J., de Jong, Arjan S., van Loon, Anton M., Pas, Suzan D., Pontesilli, Oscar, Rossen, John W., Swanink, Caroline M.A., Thijsen, Steven, van der Zanden, Adri G.M., van der Avoort, Harrie G.A.M., Koopmans, Marion P.G., and Meijer, Adam

Published
2013
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29. Detection of SARS-CoV-2 infection in the general population by three prevailing rapid antigen tests:cross-sectional diagnostic accuracy study

Author

Venekamp, Roderick P., Veldhuijzen, Irene K., Moons, Karel G.M., van den Bijllaardt, Wouter, Pas, Suzan D., Lodder, Esther B., Molenkamp, Richard, Igloi, Zsofi, Wijers, Constantijn, dos Santos, Claudy Oliveira, Debast, Sylvia B., Bruins, Marjan J., Polad, Khaled, Nagel-Imming, Carla R.S., Han, Wanda G.H., van de Wijgert, Janneke H.H.M., van den Hof, Susan, Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., Moons, Karel G.M., van den Bijllaardt, Wouter, Pas, Suzan D., Lodder, Esther B., Molenkamp, Richard, Igloi, Zsofi, Wijers, Constantijn, dos Santos, Claudy Oliveira, Debast, Sylvia B., Bruins, Marjan J., Polad, Khaled, Nagel-Imming, Carla R.S., Han, Wanda G.H., van de Wijgert, Janneke H.H.M., van den Hof, Susan, and Schuit, Ewoud

Abstract

Background: Rapid antigen diagnostic tests (Ag-RDTs) are the most widely used point-of-care tests for detecting SARS-CoV-2 infection. Since the accuracy may have altered by changes in SARS-CoV-2 epidemiology, indications for testing, sampling and testing procedures, and roll-out of COVID-19 vaccination, we evaluated the performance of three prevailing SARS-CoV-2 Ag-RDTs. Methods: In this cross-sectional study, we consecutively enrolled individuals aged >16 years presenting for SARS-CoV-2 testing at three Dutch public health service COVID-19 test sites. In the first phase, participants underwent either BD-Veritor System (Becton Dickinson), PanBio (Abbott), or SD-Biosensor (Roche Diagnostics) testing with routine sampling procedures. In a subsequent phase, participants underwent SD-Biosensor testing with a less invasive sampling method (combined oropharyngeal-nasal [OP-N] swab). Diagnostic accuracies were assessed against molecular testing. Results: Six thousand nine hundred fifty-five of 7005 participants (99%) with results from both an Ag-RDT and a molecular reference test were analysed. SARS-CoV-2 prevalence and overall sensitivities were 13% (188/1441) and 69% (129/188, 95% CI 62–75) for BD-Veritor, 8% (173/2056) and 69% (119/173, 61–76) for PanBio, and 12% (215/1769) and 74% (160/215, 68–80) for SD-Biosensor with routine sampling and 10% (164/1689) and 75% (123/164, 68–81) for SD-Biosensor with OP-N sampling. In those symptomatic or asymptomatic at sampling, sensitivities were 72–83% and 54–56%, respectively. Above a viral load cut-off (≥5.2 log10 SARS-CoV-2 E-gene copies/mL), sensitivities were 86% (125/146, 79–91) for BD-Veritor, 89% (108/121, 82–94) for PanBio, and 88% (160/182, 82–92) for SD-Biosensor with routine sampling and 84% (118/141, 77–89) with OP-N sampling. Specificities were >99% for all tests in most analyses. Sixty-one per cent of false-negative Ag-RDT participants returned for testing within 14 days (median: 3 days, interquar

Published
2022

30. Head-to-head comparison of the accuracy of saliva and nasal rapid antigen SARS-CoV-2 self-testing:cross-sectional study

Author

Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Stohr, Joep J.J.M., Lodder, Esther B., Hellwich, Marloes, Molenkamp, Richard, Igloi, Zsofia, Wijers, Constantijn, Vroom, Irene H., Nagel-Imming, Carla R.S., Han, Wanda G.H., Kluytmans, Jan A.J.W., van den Hof, Susan, van de Wijgert, Janneke H.H.M., Moons, Karel G.M., Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Stohr, Joep J.J.M., Lodder, Esther B., Hellwich, Marloes, Molenkamp, Richard, Igloi, Zsofia, Wijers, Constantijn, Vroom, Irene H., Nagel-Imming, Carla R.S., Han, Wanda G.H., Kluytmans, Jan A.J.W., van den Hof, Susan, van de Wijgert, Janneke H.H.M., and Moons, Karel G.M.

Abstract

Background: The diagnostic accuracy of unsupervised self-testing with rapid antigen diagnostic tests (Ag-RDTs) is mostly unknown. We studied the diagnostic accuracy of a self-performed SARS-CoV-2 saliva and nasal Ag-RDT in the general population. Methods: This large cross-sectional study consecutively included unselected individuals aged ≥ 16 years presenting for SARS-CoV-2 testing at three public health service test sites. Participants underwent molecular test sampling and received two self-tests (the Hangzhou AllTest Biotech saliva self-test and the SD Biosensor nasal self-test by Roche Diagnostics) to perform themselves at home. Diagnostic accuracy of both self-tests was assessed with molecular testing as reference. Results: Out of 2819 participants, 6.5% had a positive molecular test. Overall sensitivities were 46.7% (39.3–54.2%) for the saliva Ag-RDT and 68.9% (61.6–75.6%) for the nasal Ag-RDT. With a viral load cut-off (≥ 5.2 log10 SARS-CoV-2 E-gene copies/mL) as a proxy of infectiousness, these sensitivities increased to 54.9% (46.4–63.3%) and 83.9% (76.9–89.5%), respectively. For the nasal Ag-RDT, sensitivities were 78.5% (71.1–84.8%) and 22.6% (9.6–41.1%) in those symptomatic and asymptomatic at the time of sampling, which increased to 90.4% (83.8–94.9%) and 38.9% (17.3–64.3%) after applying the viral load cut-off. In those with and without prior SARS-CoV-2 infection, sensitivities were 36.8% (16.3–61.6%) and 72.7% (65.1–79.4%). Specificities were > 99% and > 99%, positive predictive values > 70% and > 90%, and negative predictive values > 95% and > 95%, for the saliva and nasal Ag-RDT, respectively, in most analyses. Most participants considered the self-performing and result interpretation (very) easy for both self-tests. Conclusions: The Hangzhou AllTest Biotech saliva self Ag-RDT is not reliable for SARS-CoV-2 detection, overall, and in all studied subgroups. The SD Biosensor nasal self Ag-RDT had high sensitivity in individuals wit

Published
2022

31. SARS-CoV-2 Alpha-Variant Outbreak Amongst a Partially Vaccinated Long-Term Care Facility Population in The Netherlands—Phylogenetic Analysis and Infection Control Observations

Author

MMB Staf diagnostiek, Medische Microbiologie, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, MMB Medische Staf, Van der Moeren, Nathalie, Weterings, Veronica A.T.C., Pas, Suzan D., Verweij, Jaco J., van den Bijllaardt, Wouter, Geurts, Joyce, Zwart, Vivian F., Lodder, Esther B., Kluytmans, Jan A.J.W., Murk, Jean Luc, Stohr, Joep J.J.M., MMB Staf diagnostiek, Medische Microbiologie, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, MMB Medische Staf, Van der Moeren, Nathalie, Weterings, Veronica A.T.C., Pas, Suzan D., Verweij, Jaco J., van den Bijllaardt, Wouter, Geurts, Joyce, Zwart, Vivian F., Lodder, Esther B., Kluytmans, Jan A.J.W., Murk, Jean Luc, and Stohr, Joep J.J.M.

Published
2022

32. Head-to-head comparison of the accuracy of saliva and nasal rapid antigen SARS-CoV-2 self-testing: cross-sectional study

Author

Epi Methoden Team 4, JC onderzoeksprogramma Methodology, HAG Onderzoek, Child Health, Infection & Immunity, JC onderzoeksprogramma Infectious Diseases, Medische Microbiologie, Epi Infectieziekten Team 1b, Epi Methoden, Cancer, Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Stohr, Joep J.J.M., Lodder, Esther B., Hellwich, Marloes, Molenkamp, Richard, Igloi, Zsofia, Wijers, Constantijn, Vroom, Irene H., Nagel-Imming, Carla R.S., Han, Wanda G.H., Kluytmans, Jan A.J.W., van den Hof, Susan, van de Wijgert, Janneke H.H.M., Moons, Karel G.M., Epi Methoden Team 4, JC onderzoeksprogramma Methodology, HAG Onderzoek, Child Health, Infection & Immunity, JC onderzoeksprogramma Infectious Diseases, Medische Microbiologie, Epi Infectieziekten Team 1b, Epi Methoden, Cancer, Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Stohr, Joep J.J.M., Lodder, Esther B., Hellwich, Marloes, Molenkamp, Richard, Igloi, Zsofia, Wijers, Constantijn, Vroom, Irene H., Nagel-Imming, Carla R.S., Han, Wanda G.H., Kluytmans, Jan A.J.W., van den Hof, Susan, van de Wijgert, Janneke H.H.M., and Moons, Karel G.M.

Published
2022

33. Diagnostic accuracy of covid-19 rapid antigen tests with unsupervised self-sampling in people with symptoms in the omicron period: cross sectional study

Author

Epi Methoden Team 4, JC onderzoeksprogramma Methodologie, HAG Onderzoek, Child Health, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Epidemiology & Health Economics, Medische Microbiologie, Epi Infectieziekten Team 1b, Epi Methoden, Cancer, Schuit, Ewoud, Venekamp, Roderick P., Hooft, Lotty, Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Zwart, Vivian F., Lodder, Esther B., Hellwich, Marloes, Koppelman, Marco, Molenkamp, Richard, Wijers, Constantijn J.H., Vroom, Irene H., Smeets, Leonard C., Nagel-Imming, Carla R.S., Han, Wanda G.H., van den Hof, Susan, Kluytmans, Jan A.J.W., van de Wijgert, Janneke H.H.M., Moons, Karel G.M., Epi Methoden Team 4, JC onderzoeksprogramma Methodologie, HAG Onderzoek, Child Health, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Epidemiology & Health Economics, Medische Microbiologie, Epi Infectieziekten Team 1b, Epi Methoden, Cancer, Schuit, Ewoud, Venekamp, Roderick P., Hooft, Lotty, Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Zwart, Vivian F., Lodder, Esther B., Hellwich, Marloes, Koppelman, Marco, Molenkamp, Richard, Wijers, Constantijn J.H., Vroom, Irene H., Smeets, Leonard C., Nagel-Imming, Carla R.S., Han, Wanda G.H., van den Hof, Susan, Kluytmans, Jan A.J.W., van de Wijgert, Janneke H.H.M., and Moons, Karel G.M.

Published
2022

34. Detection of SARS-CoV-2 infection in the general population by three prevailing rapid antigen tests: cross-sectional diagnostic accuracy study

Author

HAG Onderzoek, Child Health, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Epi Methoden, Cancer, JC onderzoeksprogramma Methodologie, Epi Infectieziekten Team 1b, Epi Methoden Team 4, Venekamp, Roderick P, Veldhuijzen, Irene K, Moons, Karel G M, van den Bijllaardt, Wouter, Pas, Suzan D, Lodder, Esther B, Molenkamp, Richard, Igloi, Zsofi, Wijers, Constantijn, Dos Santos, Claudy Oliveira, Debast, Sylvia B, Bruins, Marjan J, Polad, Khaled, Nagel-Imming, Carla R S, Han, Wanda G H, van de Wijgert, Janneke H H M, van den Hof, Susan, Schuit, Ewoud, HAG Onderzoek, Child Health, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Epi Methoden, Cancer, JC onderzoeksprogramma Methodologie, Epi Infectieziekten Team 1b, Epi Methoden Team 4, Venekamp, Roderick P, Veldhuijzen, Irene K, Moons, Karel G M, van den Bijllaardt, Wouter, Pas, Suzan D, Lodder, Esther B, Molenkamp, Richard, Igloi, Zsofi, Wijers, Constantijn, Dos Santos, Claudy Oliveira, Debast, Sylvia B, Bruins, Marjan J, Polad, Khaled, Nagel-Imming, Carla R S, Han, Wanda G H, van de Wijgert, Janneke H H M, van den Hof, Susan, and Schuit, Ewoud

Published
2022

35. Self-testing for the detection of SARS-CoV-2 infection with rapid antigen tests for people with suspected COVID-19 in the community

Author

Epi Infectieziekten Team 1, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Medische Microbiologie, Stohr, Joep J.J.M., Zwart, Vivian F., Goderski, Gabriel, Meijer, Adam, Nagel-Imming, Carla R.S., Kluytmans-van den Bergh, Marjolein F.Q., Pas, Suzan D., van den Oetelaar, Femke, Hellwich, Marloes, Gan, Kim H., Rietveld, Ariene, Verweij, Jaco J., Murk, Jean Luc, van den Bijllaardt, Wouter, Kluytmans, Jan A.J.W., Epi Infectieziekten Team 1, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Medische Microbiologie, Stohr, Joep J.J.M., Zwart, Vivian F., Goderski, Gabriel, Meijer, Adam, Nagel-Imming, Carla R.S., Kluytmans-van den Bergh, Marjolein F.Q., Pas, Suzan D., van den Oetelaar, Femke, Hellwich, Marloes, Gan, Kim H., Rietveld, Ariene, Verweij, Jaco J., Murk, Jean Luc, van den Bijllaardt, Wouter, and Kluytmans, Jan A.J.W.

Published
2022

37. Accuracy of COVID-19 self-tests with unsupervised nasal or nasal plus oropharyngeal self-sampling in symptomatic individuals in the Omicron period

Author

Schuit, Ewoud, primary, Venekamp, Roderick P, additional, Hooft, Lotty, additional, Veldhuijzen, Irene K, additional, van den Bijllaardt, Wouter, additional, Pas, Suzan D, additional, Zwart, Vivian F, additional, Lodder, Esther B, additional, Hellwich, Marloes, additional, Koppelman, Marco, additional, Molenkamp, Richard, additional, Wijers, Constantijn, additional, Vroom, Irene H, additional, Smeets, Leonard C, additional, Nagel-Imming, Carla RS, additional, Han, Wanda GH, additional, van den Hof, Susan, additional, Kluytmans, Jan AJW, additional, van de Wijgert, Janneke HM, additional, and Moons, Karel GM, additional

Published
2022
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38. Additional file 1 of Detection of SARS-CoV-2 infection in the general population by three prevailing rapid antigen tests: cross-sectional diagnostic accuracy study

Author

Venekamp, Roderick P., Veldhuijzen, Irene K., Moons, Karel G. M., van den Bijllaardt, Wouter, Pas, Suzan D., Lodder, Esther B., Molenkamp, Richard, Igloi, Zsofi, Wijers, Constantijn, dos Santos, Claudy Oliveira, Debast, Sylvia B., Bruins, Marjan J., Polad, Khaled, Nagel-Imming, Carla R. S., Han, Wanda G. H., van de Wijgert, Janneke H. H. M., van den Hof, Susan, and Schuit, Ewoud

Abstract

Additional file 1 : Suppl. Table S1. Baseline characteristics of the study population included in the PanBio test with less invasive sampling technique evaluation. Suppl. Table S2. Diagnostic accuracy variables of PanBio-molecular reference standard test comparison with less invasive sampling. Values are percentages (95% confidence interval) unless stated otherwise. Suppl. Table S3. Diagnostic accuracy variables of additional secondary analyses of three rapid antigen tests, with different sampling methods. Values are percentages (95% confidence interval) unless stated otherwise. Suppl. Table S4. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the BD VeritorTM System by Becton Dickinson (���BD-Veritor���) using routine sampling (OP-N). Suppl. Table S5. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the Roche/SD Biosensor by Roche Diagnostics (���SD-Biosensor���) using routine sampling (NP). Suppl. Table S6. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the Roche/SD Biosensor by Roche Diagnostics (���SD-Biosensor���) using less invasive sampling (OP-N). Suppl. Table S7. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the PanBio by Abbot (���PanBio���) using routine sampling (NP). Suppl. Table S8. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the PanBio by Abbot (���PanBio���) using less invasive sampling (OP-N). Suppl. Table S9. GISAID data contributors acknowledgement table. Suppl. Material 1. Short questionnaire (translated from Dutch). Suppl. Material 2. Specimen collection, SARS-CoV-2 diagnostic testing, and SARS-CoV-2 virus culture procedures.

Published
2022
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39. Additional file 1 of Head-to-head comparison of the accuracy of saliva and nasal rapid antigen SARS-CoV-2 self-testing: cross-sectional study

Author

Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Stohr, Joep J. J. M., Lodder, Esther B., Hellwich, Marloes, Molenkamp, Richard, Igloi, Zsofia, Wijers, Constantijn, Vroom, Irene H., Nagel-Imming, Carla R. S., Han, Wanda G. H., Kluytmans, Jan A. J. W., van den Hof, Susan, van de Wijgert, Janneke H. H. M., and Moons, Karel G. M.

Abstract

Additional file 1: Material 1. Short questionnaire after performing both self-tests at home. Material 2. Short questionnaire provided 10 days after test site visit. Material 3. Specimen collection, SARS-CoV-2 diagnostic testing, and SARS-CoV-2 virus culture procedures. Table S1. 2x2 tables for primary and secondary analyses of the molecular reference test – saliva Ag-RDT comparison. Table S2. 2x2 tables for primary and secondary analyses of the molecular reference test – nasal Ag-RDT comparison. Table S3. Follow-up information of participants that were tested again within 10 days after the initial test. Fig. S1. Sensitivities with 95% confidence intervals of the Ag-RDT-molecular reference standard test comparisons at viral load cut-off for infectiousness. Fig. S2. Distribution of viral loads in participants with a positive molecular reference test, stratified by symptomatology at time of sampling. Fig. S3. Distribution of change scores in difficulty of taking a sample and difficulty of reading the test result when comparing difficulty ratings of the saliva Ag-RDT and the nasal Ag-RDT.

Published
2022
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40. Accuracy and usability of saliva and nasal rapid antigen self-testing for detection of SARS-CoV-2 infection in the general population: a head-to-head comparison

Author

Schuit, Ewoud, primary, Venekamp, Roderick P, additional, Veldhuijzen, Irene K, additional, van den Bijllaardt, Wouter, additional, Pas, Suzan D, additional, Stohr, Joep J J M, additional, Lodder, Esther B, additional, Hellwich, Marloes, additional, Molenkamp, Richard, additional, Igloi, Zsofia, additional, Wijers, Constantijn, additional, Vroom, Irene H, additional, Nagel-Imming, Carla R S, additional, Han, Wanda G H, additional, Kluytmans, Jan AJW, additional, van den Hof, Susan, additional, van de Wijgert, Janneke H H M, additional, and Moons, Karel G M, additional

Published
2021
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41. Isolation of MERS coronavirus from dromedary camel, Qatar, 2014

Author

Raj, V. Stalin, Farag, Elmoubasher A.B.A., Reusken, Chantal B.E.M., Lamers, Mart M., Pas, Suzan D., Voermans, Jolanda, Smits, Saskia L., Osterhaus, Albert D.M.E., Al-Mawlawi, Naema, Al-Romaihi, Hamad E., AlHajri, Mohd M., El-Sayed, Ahmed M., Mohran, Khaled A., Ghobashy, Hazem, Alhajri, Farhoud, Al-Thani, Mohamed, Al-Marri, Salih A., El-Maghraby, Mamdouh M., Koopmans, Marion P.G., and Haagmans, Bart L.

Subjects
Camels -- Health aspects, Health
Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel coronavirus that can cause severe lower respiratory tract infection in humans (1,2). MERS-CoV clusters with viruses in the genus Beta-coronavirus; the [...]

Published
2014
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46. Diagnostic accuracy of three prevailing rapid antigen tests for detection of SARS-CoV-2 infection in the general population: cross sectional study

Author

Venekamp, Roderick P, primary, Veldhuijzen, Irene K, additional, Moons, Karel GM, additional, van den Bijllaardt, Wouter, additional, Pas, Suzan D, additional, Lodder, Esther B, additional, Molenkamp, Richard, additional, Igloi, Zsofia, additional, Wijers, Constantijn, additional, Oliviera dos Santos, Claudy, additional, Debast, Sylvia B, additional, Bruins, Marjan J., additional, Polad, Khaled, additional, Nagel-Imming, Carla RS, additional, Han, Wanda, additional, van de Wijgert, Janneke HHM, additional, van den Hof, Susan, additional, and Schuit, Ewoud, additional

Published
2021
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50. Diagnostic accuracy of rapid antigen tests in asymptomatic and presymptomatic close contacts of individuals with confirmed SARS-CoV-2 infection: cross sectional study

Author

Schuit, Ewoud, primary, Veldhuijzen, Irene K, additional, Venekamp, Roderick P, additional, van den Bijllaardt, Wouter, additional, Pas, Suzan D, additional, Lodder, Esther B, additional, Molenkamp, Richard, additional, GeurtsvanKessel, Corine H, additional, Velzing, Jans, additional, Huisman, Robin C, additional, Brouwer, Lieke, additional, Boelsums, Timo L, additional, Sips, Gregorius J, additional, Benschop, Kimberly S M, additional, Hooft, Lotty, additional, van de Wijgert, Janneke H H M, additional, van den Hof, Susan, additional, and Moons, Karel G M, additional

Published
2021
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