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A novel approach was used for develop and validate a rapid, accurate, and an isocratic RP-HPLC method with PDA detector for the estimation of ixazomib drug in pharmaceutical dosage forms. Ixazomib was separated using Agilent 4.6 X150 mm, 5 pm analytical column, a Waters HPLC system (USA) and a mobile phase consisting of water and acetonitrile in the ratio of 40:60 V/V. The flow rate was set to 0.7 mL/min with 10pL injection volume. The column was maintained at ambient temperature, detector was set at wavelength of 274 nm. The retention time of ixazomib was found to be 2.17 min. The system suitability parameters for ixazomib such as theoretical plates and tailing factor were found to be 4146. Linearity was established for ixazomib in the range of 50-250 pg / ml concentration levels with correlation coefficients (r2) of 0.999.The intra-and inter-day precision % RSD values were found to be 0.47 and 0.31, respectively. The limit of detection (LOD) and limit of quantification (LOQ) values were found to be 2.03 and 6.17 pg/mL respectively. The method was validated for all of the above parameters according to the International Conference on Harmonization (ICH) guidelines. This method can be used for estimation and analysis of ixazomib drug in active pharmaceutical ingredients and pharmaceuticals. [ABSTRACT FROM AUTHOR]

Seven 4-arylbenzelidene-2-((5-fluoro-1H-indol-1-yl)methyl)oxazol-5(4H)-one derivatives were synthesized by the condensation of 2-(2-(5-fluoro-1H-indol-1-yl) acetoamide) acetic acid, substituted aromatic aldehydes with acetic anhydride and sodium acetate in the presence of MgO/Al2O3 under reflux using nickel ferrite nanoparticles. Seven of the compounds are new derivatives. 2-(2-(5-fluoro-1H-indol-1-yl)acetoamide) acetic acid was obtained from 2-(5-fluoro-1H-indol-1-yl)acetyl chloride with lysine in the presence of NaOH and HCl in the ice cold solution. 2-(5-fluoro-1H-indol-1-yl)acetyl chloride can be prepared from 5-fluoro indole with chloroacetyl chloride in triethylamine and dichloromethane. The structures of the compounds were evaluated based on ¹H-NMR, 13C-NMR and LC-MS and by elemental analysis. These compounds were screened by antioxidant as well as anti-microbial activity. [ABSTRACT FROM AUTHOR]

ABSTRACT. An efficient and convenient procedure has been described for one-pot multi-component synthesis of tetrahydrobenzo[b]pyrans known as 2-amino-7,7-dimethyl-5-oxo-4-phenyl-5,6,7,8-tetrahydro-4H-chromene-3-carbonitrile which can be obtained from the reaction of substituted aromatic aldehydes, dimedone, malonitrile, in the presence of base such as potassium tertiary butoxide and THF in methanol as solvent at RT condition. All the compounds were examined by advanced spectroscopic data ( ¹H NMR, 13C NMR and LCMS) and the structural determination was evaluated by elemental analysis. In addition to this, all the newly synthesized compounds were examined for their antibacterial activities and antifungal activity by disc diffusion method against the organism of Aspergillus niger and Candida ablicans L. [ABSTRACT FROM AUTHOR]

A simple and inexpensive method was developed using high performance liquid chromatography with PDA detection for determination of granisetron hydrochloride and related impurities (2-methyl-N- [(1R,3r,5S)-9-methyl-9-azabicyclo[3.3.1]non-3-yl]-2H-indazole-3-carboxamide, 1-Methyl-N-[(1R,3r,5S)- 9-methyl-9-azabicyclo[3.3.1]non-3-yl]-1H-indazole-3-carboxamide hydrochloride, N-[(1R,3r,5S)-9-azabicyclo[3.3.1]non-3-yl]-1-methyl-1H-indazole-3-carboxamide and 1-methyl-1H-indazole-3-carboxylic acid. The chromatographic separations were achieved on (250×4.6 mm), 5.0 µm make: Waters, Symmetry Shield C8 column employing acetonitrile: 2% V/V H3PO4 in MilliQ water + 0.1% V/V hexylamine in water, 800:200 V/V (pH adjusted to 7.5 using triethylamine) mobile phase with isocratic programme. A flow rate of 1.5 mL/min was chosen. Four impurities were eluted within 20 minutes. The column temperature was maintained at 40°C and a detector wavelength of 300 nm was employed. The method was successfully validated by establishing system suitability, specificity, linearity, accuracy, limit of detection and limit of quantification. [ABSTRACT FROM AUTHOR]

Zinc oxide nanoparticles (ZnO NPs) were synthesized by a precipitation method, and a new charring–foaming agent (CFA) N-ethanolamine triazine-piperazine, melamine polymer (ETPMP) was synthesized via nucleophilic substitution reaction by using cyanuric chloride, ethanolamine, piperazine, and melamine as precursor molecules. FTIR and energy-dispersive X-ray spectroscopy (EDS) studies were employed to characterize and confirm the synthesized ETPMP structure. New intumescent flame retardant epoxy coating compositions were prepared by adding ammonium polyphosphate (APP), ETPMP, and ZnO NPs into an epoxy resin. APP and ETPMP were fixed in a 2:1 w/w ratio and used as an intumescent flame-retardant (IFR) system. ZnO NPs were loaded as a synergistic agent in different amounts into the IFR coating system. The synergistic effects of ZnO NPs on IFR coatings were systematically evaluated by limited oxygen index (LOI) tests, vertical burning tests (UL-94 V), TGA, cone calorimeter tests, and SEM. The obtained results revealed that a small amount of ZnO NPs significantly increased the LOI values of the IFR coating and these coatings had a V-0 ratings in UL-94 V tests. From the TGA data, it is clear that the addition of ZnO NPs could change the thermal degradation behaviors of coatings with increasing char residue percentage at high temperatures. Cone calorimeter data reported that ZnO NPs could decrease the combustion parameters including peak heat release rates (PHRRs), and total heat release (THR) rates. The SEM results showed that ZnO NPs could enhance the strength and the compactness of the intumescent char, which restricted the flow of heat and oxygen. [ABSTRACT FROM AUTHOR]