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The extreme conditions linked with abiotic stresses have greatly affected soil and plant health. The diverse biochemical activities occurring in the soil environment have been attributed to shaping the dynamics of plant–soil microbiomes by contributing to microbial lifestyles and enhancing microbial functional properties to boost plant tolerance to abiotic-induced stresses. Soil microbiomes play crucial roles in enhancing plant nutrition and abiotic stress management through diverse mechanisms. With the current insights into the use of engineered soil microbes as single or combined inoculants, their use has contributed to plant fitness and stability under different environmental stress conditions by activating plant defense mechanisms, enzyme production (lowering free radicals resulting in plant oxidative stress), protein regulation, and the production of growth factors. The detection of certain genes involved in the growth factors can underline microbial functions in mitigating plant stress. Hence, the projections for sustainable eco-friendly agriculture with the possible exploration of beneficial rhizosphere microbes to manage the effect of abiotic stress on plant nutrition remain critical points of discussion recently, with prospects for ensuring food security. Therefore, this review focuses on the impacts of soil microbiomes in abiotic stress mitigation for enhancing plant nutrition.

Abstract Background Infection risk was significant for front-line nurses during the Covid-19 outbreak. The pandemic presented several ethical difficulties and sapped nurses’ drive to labor harder for longer periods. This study evaluates registered nurses’ perceptions of Covid-19 infection risk, ethical dilemmas, and motivating factors. Materials and methods During March and April 2022, 400 registered nurses from a newly established tertiary care hospital participated in this cross-sectional exploratory survey. The risk assessment scale, motivation to work scale, and ethical dilemma scale were used to assess the perceived risk of infection, motivational factors and ethical challenges experienced by the nurses. Appropriate descriptive and inferential statistics were applied to compute the results. Results 76.4% of nurses feared working as a nurse put them at higher risk of infection. Besides the fear of contracting infection, nurses believed they were the source of infection to family members (70.8%) and people around (67.5%). 63.3% of nurses agree that they do not have the right to refuse treatment and every patient has the right to receive optimal care, regardless of age, gender, and medical conditions. Professional obligation to treat patients (72.3%) and sound professional knowledge and experience (83.5%) are important motivating factors to work during the pandemic. Multilinear regression analysis revealed that professional education (95% CI, 3.845 − 0.694, p = 0.005), Covid-19 positive status (95% CI,0.455-2.756, p = 0.006), and post-Covid-19 hospitalization (95% CI, 1.395–6.978, p = 0.003) and duration of hospitalization (95% CI, 0.754-0.058, p = 0.022) are independent predictors of higher perceived risk of infection among nurses. Conclusions During the pandemic, nurses were afraid to work and faced personal and family risks of contracting the virus. Despite these challenges, they still feel a strong sense of commitment and dedication to providing the best possible care. Nurse administrators need to create a supportive environment that follows ethical principles and meets the needs of nurses to boost their motivation and encourage them to continue working for longer periods.

Abstract Synbiotics are employed as feed additives in animal production as an alternate to antibiotics for sustaining the gut microbiota and providing protection against infections. Dairy calves require a healthy diet and management to ensure a better future for the herd of dairy animals. Therefore, the present study was carried out to investigate the effect of synbiotics formulation on growth performance, nutrient digestibility, fecal bacterial count, metabolites, immunoglobulins, blood parameters, antioxidant enzymes and immune response of pre-ruminant Murrah buffalo calves. Twenty-four apparently healthy calves (5 days old) were allotted into four groups of six calves each. Group I (control) calves were fed a basal diet of milk, calf starter and berseem with no supplements. Group II (SYN1) calves were fed with 3 g fructooligosaccharide (FOS) + Lactobacillus plantarum CRD-7 (150 ml). Group III (SYN2) calves were fed with 6 g FOS + L. plantarum CRD-7 (100 ml), whereas calves in group IV (SYN3) received 9 g FOS + L. plantarum CRD-7 (50 ml). The results showed that SYN2 had the highest (P

Methane is one of the main greenhouse gases emitted by ruminants around the world. It is essential to investigate novel approaches to increasing animal production while reducing greenhouse gas emissions from ruminants. This study was conducted to examine the effect of methane inhibitors, such as nitrate, linseed oil, and anthraquinone, on nutritional digestibility, rumen fermentation processes, and methane emission in Sahiwal and Gir cattle calves. Twelve calves (6–12 months old), six of each Sahiwal and Gir breed, were selected and divided into four groups; Sahiwal control (C) and treated (T) calves; Gir control (C) and treated calves (T) of three calves each based on average body weight. Switch over a design was used as for periods 1 and 2. Animals in all groups were fed chopped oat fodder, wheat straw, and a concentrate mixture. Additionally, treated groups were fed a ration with potassium nitrate (1%), linseed oil (0.5%), and anthraquinone (4 ppm). The results revealed that the addition of methane inhibitors had no impact on nutrient intake and apparent digestibility. The levels of propionate, ammonia nitrogen, and total nitrogen were increased significantly (p < 0.05), while butyrate decreased in the treated groups of both breeds. However, there was no change in acetate and pH between the groups. Methane emission (g/d) was lower (p < 0.05) in the treated groups as compared to the control group. This study concludes that supplementation of methane inhibitors in calves feed can be utilized to lower methane emissions without affecting the intake and digestibility of nutrients. Combining diverse dietary mitigation strategies could be an effective way to mitigate methane emissions to reduce global warming while minimizing any negative impacts on ruminants to accomplish sustainable animal production.

Bamboo biomass is a potential source of monomeric sugars containing a high cellulose content with a low amount of lignin. However, for efficient hydrolysis, an effective biomass pretreatment technique is required to minimize the lignin content and other barrier components. In the present study, bamboo biomass was treated with different physical, chemical, biological, and combined treatments to reduce the lignin content. Among all the pretreatments, the maximum lignin removal amount (14.5%) was obtained with the combined chemical and biological treatment under 2% NaOH + 1% H2O2 + WDP2 fungal culture (5 plugs) conditions. In addition, the ligninolytic fungus and NaOH pretreatment was primarily effective in removing lignins, whereas the H2O2 pretreatment efficiently minimized cellulose crystallinity. Scanning electron microscopy and Fourier-transform infrared spectroscopy was utilized to analyze the structural changes of the raw and treated biomass. The structural analysis indicated that all the treatments caused disruption in the biomass structure and reduced the compactness of the biomass, which facilitated the biomass conversion during the hydrolysis process. The findings of the present study indicated effective pretreatment methods in overcoming the recalcitrancy of potential lignocellulosic biomass for maximum hydrolysis.

Abstract Exploring innovative methods to provide essential nutrients and reducing ruminant greenhouse gas emission is crucial for animal production and diminishing global warming. This study was conducted to examine the efficacy of Moringa oleifera leaves (ML) in ruminants at 0%, 5%, 10%, 15%, 20%, 30% and 40% level in different roughage (R) and concentrate (C) (80R:20C, 70R:30C and 60R:40C) under in vitro conditions. Chemical composition of ML, concentrate mixture and berseem were estimated. Rumen fermentation parameters of male goat kids viz., total gas production, CH4, true dry matter digestibility (TDMD), organic matter digestibility (TOMD), partial fraction (PF), microbial biomass (MBP), ammonia (N), acetate, propionate, butyrate and acetate propionate ratio were observed under in vitro conditions. Results revealed that crude protein, organic matter and ethyl ether content were higher in ML as compared to concentrate mixture and berseem. Magnesium and iron content were also higher in ML as compared to concentrate and berseem. Total gas production, digestibility of DM and OM, MBP, acetate and propionate level were improved (P

Abstract Background Since the World’s population is increasing, it’s critical to boost agricultural productivity to meet the rising demand for food and reduce poverty. Fertilizers are widely used in traditional agricultural methods to improve crop yield, but they have a number of negative environmental consequences such as nutrient losses, decrease fertility and polluted water and air. Researchers have been focusing on alternative crop fertilizers mechanisms to address these issues in recent years and nanobiofertilizers have frequently been suggested. “Nanophos” is a biofertilizer and contains phosphate-solubilising bacteria that solubilises insoluble phosphate and makes it available to the plants for improved growth and productivity as well as maintain soil health. This study evaluated the impact of nanophos on the growth and development of maize plants and its rhizospheric microbial community such as NPK solubilising microbes, soil enzyme activities and soil protein under field condition after 20, 40 and 60 days in randomized block design. Results Maize seeds treated with nanophos showed improvement in germination of seeds, plant height, number of leaves, photosynthetic pigments, total sugar and protein level over control. A higher activity of phenol, flavonoid, antioxidant activities and yield were noticed in nanophos treated plants over control. Positive shift in total bacterial count, nitrogen fixing bacteria, phosphate and potassium solubilizers were observed in the presence of nanophos as compared to control. Soil enzyme activities were significantly (P

Abstract This study explores the comparative effect of conventional and organic treatments on the rhizosphere microbiome of Mangifera indica cv. Dashehari. The long-term exposures (about 20 years) were monitored under a subtropical ecosystem. Based on plant growth properties and acetylene reduction assay, 12 bacterial isolates (7 from G1-organic and 5 from G2-conventional systems) were identified as Pseudomonas and Bacillus spp. In the conventional system, dehydrogenase activity significantly decreased (0.053 µg TPF formed g−1 of soil h−1) and adversely affected the bacterial diversity composition. In comparison, organic treatments had a good impact on dehydrogenase activity (0.784 µg TPF formed g−1 of soil h−1), alkaline phosphatase (139.25 µg PNP g−1 soil h−1), and bacterial community composition. The Metagenomics approach targeted the V3 and V4 regions to see the impact in the phylum, order, family, genus, and species for both the treatments. Results showed that phylum Acidobacteria (13.6%), Firmicutes (4.84%), and Chloroflexi (2.56%) were dominating in the G2 system whereas phylum Bacteroides (14.55%), Actinobacteria (7.45%), and Proteobacteria (10.82%) were abundantly dominated in the G1 system. Metagenome sequences are at the NCBI-GenBank sequence read archive with SRX8289747 (G1) and SRX8289748 (G2) in the study PRJNA631113. Results indicated that conventional and organic conditions affect rhizosphere microbiome and their environment.

Biotic stress is caused by harmful microbes that prevent plants from growing normally and also having numerous negative effects on agriculture crops globally. Many biotic factors such as bacteria, fungi, virus, weeds, insects, and nematodes are the major constrains of stress that tends to increase the reactive oxygen species that affect the physiological and molecular functioning of plants and also led to the decrease in crop productivity. Bacterial and fungal endophytes are the solution to overcome the tasks faced with conventional farming, and these are environment friendly microbial commodities that colonize in plant tissues without causing any damage. Endophytes play an important role in host fitness, uptake of nutrients, synthesis of phytohormone and diminish the injury triggered by pathogens via antibiosis, production of lytic enzymes, secondary metabolites, and hormone activation. They are also reported to help plants in coping with biotic stress, improving crops and soil health, respectively. Therefore, usage of endophytes as biofertilizers and biocontrol agent have developed an eco-friendly substitute to destructive chemicals for plant development and also in mitigation of biotic stress. Thus, this review highlighted the potential role of endophytes as biofertilizers, biocontrol agent, and in mitigation of biotic stress for maintenance of plant development and soil health for sustainable agriculture.

With the increase in world population, the demography of humans is estimated to be exceeded and it has become a major challenge to provide an adequate amount of food, feed, and agricultural products majorly in developing countries. The use of chemical fertilizers causes the plant to grow efficiently and rapidly to meet the food demand. The drawbacks of using a higher quantity of chemical or synthetic fertilizers are environmental pollution, persistent changes in the soil ecology, physiochemical composition, decreasing agricultural productivity and cause several health hazards. Climatic factors are responsible for enhancing abiotic stress on crops, resulting in reduced agricultural productivity. There are various types of abiotic and biotic stress factors like soil salinity, drought, wind, improper temperature, heavy metals, waterlogging, and different weeds and phytopathogens like bacteria, viruses, fungi, and nematodes which attack plants, reducing crop productivity and quality. There is a shift toward the use of biofertilizers due to all these facts, which provide nutrition through natural processes like zinc, potassium and phosphorus solubilization, nitrogen fixation, production of hormones, siderophore, various hydrolytic enzymes and protect the plant from different plant pathogens and stress conditions. They provide the nutrition in adequate amount that is sufficient for healthy crop development to fulfill the demand of the increasing population worldwide, eco-friendly and economically convenient. This review will focus on biofertilizers and their mechanisms of action, role in crop productivity and in biotic/abiotic stress tolerance.

The indiscriminate use of organophosphate insecticide chlorpyrifos in agricultural crops causes significant soil and water pollution and poses a serious threat to the global community. In this study, a microbial consortium ERM C-1 containing bacterial strains Pseudomonas putida T7, Pseudomonas aeruginosa M2, Klebsiella pneumoniae M6, and a fungal strain Aspergillus terreus TF1 was developed for the effective degradation of chlorpyrifos. Results revealed that microbial strains were not only utilizing chlorpyrifos (500 mg L–1) but also coupled with plant growth-promoting characteristics and laccase production. PGP traits, that is, IAA (35.53, 45.53, 25.19, and 25.53 μg mL–1), HCN (19.85, 17.85, 12.18, and 9.85 μg mL–1), and ammonium (14.73, 16.73, 8.05, and 10.87 μg mL–1) production, and potassium (49.53, 66.72, 46.14, and 52.72 μg mL–1), phosphate (52.37, 63.89, 33.33, and 71.89 μg mL–1), and zinc (29.75, 49.75, 49.12, and 57.75 μg mL–1) solubilization tests were positive for microbial strains T7, M2, M6, and TF1, respectively. The laccase activity by ERM C-1 was estimated as 37.53, 57.16, and 87.57 enzyme U mL–1 after 5, 10, and 15 days of incubation, respectively. Chlorpyrifos degradation was associated with ERM C-1 and laccase activity, and the degree of enzyme activity was higher in the consortium than in individual strains. The biodegradation study with developed consortium ERM C-1 showed a decreased chlorpyrifos concentration from the 7th day of incubation (65.77% degradation) followed by complete disappearance (100% degradation) after the 30th day of incubation in the MS medium. First-order degradation kinetics with a linear model revealed a high k –day value and low t1/2 value in ERM C-1. The results of HPLC and GC-MS analysis proved that consortium ERM C-1 was capable of completely removing chlorpyrifos by co-metabolism mechanism.

Retailer revenue is significantly impacted by item placement in retail stores. Notably, placement of items in the premium slots (i.e., slots with increased visibility/accessibility) improves the probability of sale w.r.t. item placement in non-premium slots. Moreover, customers often tend to buy sets of items (i.e., itemsets) instead of individual purchases. In this paper, we address the problem of maximizing retailer revenue by determining the placement of itemsets in different types of slots with varied premiumness. Our key contributions are as follows. First, we introduce the notion of premiumness of retail slots and discuss the issue of itemset placement in slots with varied premiumness. Second, we propose two efficient schemes, namely ${P}$ remiumness and ${R}$ evenue-based ${I}$ temset ${P}$ lacement (PRIP) and ${P}$ remiumness and ${A}$ verage ${R}$ evenue-based ${I}$ temset ${P}$ lacement (PARIP), for placing itemsets with varying revenue in slots with varied premiumness. Third, we perform a detailed performance analysis using both real and synthetic datasets to showcase the effectiveness of our proposed schemes. We also perform a comprehensive mathematical analysis of our proposed schemes w.r.t. the complexity analysis.

Agricultural yield of major crops is low due to the injudicious use of chemical fertilizers that affects soil fertility and biodiversity severely and thereby affecting plant growth. Soil health is regulated by various factors such as physicochemical properties of the soil, availability of micro/macronutrients, soil health indicator enzymes and microbial diversity which are essential for agriculture productivity. Thus, it is required to draw attention towards an eco-friendly approach that protects the beneficial microbial population of soil. Application of different bioinoculants and agriusable nanocompounds has been reported to enhance soil quality with increased nutrient status and beneficial bacterial population, but additive effects of combined treatments on soil microbial population are largely unknown. The present study investigated the impact of nanozeolite and nanochitosan along with two Bacillus spp. on rhizospheric microbial flora and indicator enzymes to signify soil health under field conditions on maize. Soil health was ascertained by evaluating physicochemical analysis; total bacterial counts including N, P, and K solubilizing bacteria; and soil health indicator enzymes like fluorescein diacetate hydrolysis, alkaline phosphatase, β-glucosidase, dehydrogenase, amylase, and arylesterase. Change in copy number of 16S rRNA as a marker gene was used to quantify the bacterial population using quantitative PCR (qPCR) in different treatments. Our study revealed that nanocompounds with Bacillus spp. significantly (p < 0.05) enhanced total microbial count (16.89%), NPK solubilizing bacteria (46%, 41.37%, and 57.14%), and the level of soil health indicator enzymes up to twofold over control after 20, 40, and 60 days of the experiment. qPCR analysis showed a higher copy number of the 16S rRNA gene in treated samples, which also indicates a positive impact on soil bacterial population. This study presents a valuable approach to improve soil quality in combined treatments of nanocompounds and bioinoculants which can be used as a good alternative to chemical fertilizers for sustainable agriculture.

The modern agricultural system is facing the unprecedented task of contriving the extensive demand for agrarian production owing to population explosion and global climate change. The employment of Nanotechnology in agriculture has gained immense interest in recent times for the development of sustainable agricultural technologies and environmental remediation strategies. Nanotechnology pertains to the employment of nanoparticles and furnishes the potential to fabricate novel materials and products possessing improved quality. The nanomaterials may be used as; nanosensors, nanocides, nanofertilizers, nanobarcodes, and nano-remediators, which play a significant role in modern agricultural practices. However, the physical and chemical processes of nanoparticle production is neither economical nor environmentally sustainable. Therefore, the need for green or biogenic nanoparticles obtained from plants, bacteria, fungi or their metabolites has emerged as novel, sustainable, economical, biocompatible, and eco-friendly technology. In this perspective, the production and sources of biogenic nanoparticles and their implication in agro-ecosystems for crop productivity, soil health management, biocontrol, and environmental remediation have been focused on in this review. The potential development and implementation challenges are also explored.

Climate change, inadequate possessions, and land degradation all pose obstacles to modern agriculture. In the current scenario, the agriculture industry is mainly dependent on the use of chemical-based pesticides and fertilizers that impact soil health and crop productivity. Moreover, water scarcity leads farmers in drastically affected regions to use heavy metal-enriched water sources mainly originating from industrial sources for field crops irrigation. Soil pollutants can be carried into the human body via dust and water, creating negative health effects varying from simple symptoms, e.g., nausea and diarrhea and reaching death in critical cases. Thus, to clean soil contaminants, and improve soil fertility and agricultural production, alternatives to chemical fertilizers must be developed. Therefore, using beneficial microbes found in plant-associated soil microorganisms offers an effective strategy to alleviate some of these challenges, improving soil fertility, and crop yield, and protecting plants from stress conditions. Through the use of synergistic interactions, the synthetic consortium strategy seeks to improve the stability of microbial communities. In this review, synthetic consortia and their potential use in agriculture were discussed. Further, engineering new effective synthetic consortia was suggested as an effective approach in the concept of environmental bioremediation of soil pollutants and contaminants.

The development of different innovative feed resources for livestock is important to provide the essential nutrients and diminish the emission of greenhouse gases. The purpose of the present experiment was to study the response of replacing concentrate with Moringa oleifera leaves in terms of the nutrient intake, digestibility, enteric methane emissions, and performance of goat kids with a berseem-fodder-based diet under different roughage (R)-to-concentrate (C) ratios. Twenty-four goat kids (3 months of age) were distributed into four groups of six animals each, using a randomized block design (RBD). Kids of Group I (control) were fed a basal diet with 70R:30C without any tree leaf supplementation. Group II kids were fed with 60R:40C, where 10% of the concentrate mix was replaced with Moringa leaf (ML powder). In Group III, kids were fed with 70R:30C with 20% ML replacement. In Group IV, kids were fed with 80R:20C with 20% ML replacement. A metabolic trial was conducted after 180 days of feeding to assess the impact of ML on blood metabolites, antioxidant status, immunity parameters, and enteric methane emissions. The results revealed that dry matter digestibility, organic matter, and NDF were better (p < 0.05) in ML-treated kids (GII and GIII) compared to GI. Feed conversion and average daily gain were also enhanced (p < 0.05) in the treated groups as compared to controls. Total blood protein and albumin were increased in GII and GIII kids compared to GI. Plasma cholesterol levels were decreased (p < 0.001) in GII, GIII, and GIV as compared to GI. Glutathione peroxidase, catalase, and superoxide dismutase enzyme activities were also enhanced in GII, GIII, and GIV compared to controls. ML supplementation improved cell-mediated immunity and humoral immunity responses in goat kids. Enteric methane emissions decreased in the treated groups as compared to the controls. Moringa oleifera leaf may be used up to the level of 10–20% in concentrate mixes to improve digestibility, blood biochemical parameters, immunity status, and antioxidant activity in goat kids. Supplementation of ML not only enhanced the digestion and health of goat kids, but also decreased their methane emissions.

In the present study we examined the effect of nanogypsum and Pseudomonas taiwanensis strain BCRC 17751on plant and soil health using conventional and metagenomics approaches. Soil physicochemical properties and agronomical parameters of maize plants were reported to be better when applied with nanogypsum and bacterial inoculum together. When compared to control a significant increase in total bacterial counts, nitrogen, phosphorus, potassium (NPK) solubilizing bacterial population and soil enzyme activities (fluorescein diacetate, alkaline phosphatase, dehydrogenase, β-glucosidase, arylesterase and amylase) was reported in treatments. The metagenomics studies revealed dominance of beneficial bacteria such as Proteobacteria, Bacteriodetes, Planctomycetes, Acidobacteria and Nitrospirae in treated soil. On the other hand some novel bacterial diversity was also reported in treated soil which was evident from presence of taxonomically unclassified sequences. Hence, it can be concluded that combined application of nanogypsum and Pseudomonas taiwanensis in maize help in improving the structure and function of soil which affects the plant health without causing any toxic effect. However, in situ validation of the prescribed treatment is required under field conditions on different crops in order to give maximum benefits to the farmers and the environment.

In recent times, injudicious use of paclobutrazol (PBZ) in mango orchards deteriorates the soil quality and fertility by persistence nature and causes a serious ecosystem imbalance. In this study, a new Klebsiella pneumoniae strain M6 (MW228061) was isolated from mango rhizosphere and characterized as a potent plant growth promoter, biocontrol, and PBZ degrading agent. The strain M6 efficiently utilizes PBZ as carbon, energy and nitrogen source and degrades up to 98.28% (50 mgL-1 initial conc.) of PBZ at 15th day of incubation in MS medium. In the soil system first order degradation kinetics and linear model suggested 4.5 days was the theoretical half-life (t1/2 value) of PBZ with strain M6. Box Behnken design (BBD) model of Response surface methodology (RSM) showed pH 7.0, 31°C temperature, and 2.0 ml inoculum size (8 x 109 CFU mL-1) was optimized condition for maximum PBZ degradation with strain M6. Plant growth promoting attributes such as Zn, K, PO4 solubilization IAA, HCN and NH3 production of strain M6 showed positive results and were assessed quantitatively. The relation between plant growth promotion and PBZ degradation was analyzed by heat map, principal component analysis (PCA) and, clustal correlation analysis (CCA). Strain M6 was also showing a significant biocontrol activity against pathogenic fungi such as Fusarium oxysporum (MTCC-284), Colletotrichum gloeosporioides (MTCC- 2190), Pythium aphanidermatum (MTCC- 1024), Tropical race 1 (TR -1), and Tropical race 4 (TR -4). Hence, results of the study suggested that strain M6 can be utilized as an effective bio-agent to restore degraded land affected by persistent use of paclobutrazol.