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157 results on '"Parry, Nicola M. A."'

2. Dissecting aortitis in a goat associated with Pasteurella multocida and Staphylococcus spp infection.

Author

Gola, Cecilia, Fingerhood, Sai, Parry, Nicola M., and Diaz-Delgado, Josué

Subjects
STAPHYLOCOCCAL diseases, AORTITIS, VENTRICULAR septal defects, CARDIOVASCULAR diseases, PASTEURELLA multocida, EUTHANASIA of animals
Abstract

Reports of primary cardiovascular disease in goats are rare and most commonly include ventricular septal defect, valvular endocarditis, traumatic pericarditis, ionophore poisoning and nutritional cardiomyopathies. We now report the pathological findings in a 67 kg, 6-year-old, adult female Boer goat that presented with neurological signs (ie, head pressing, unsteadiness and paddling) and hyperthermia 2 days prior to death. Lack of therapeutic response to meloxicam and penicillin‒streptomycin and poor prognosis led to euthanasia of the animal. At necropsy, the main findings included severe aortic dissection with luminal thrombosis and stenosis, and pulmonary congestion and oedema. Histological examination of the aorta revealed severe chronic granulomatous and fibrosing dissecting aortitis with mineralization. Bacterial culture of the affected aortic segment resulted in isolation of a profuse growth of Pasteurella multocida and a moderate growth of Staphylococcus spp. Histopathological findings in the central nervous system were consistent with neurolisteriosis. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Characterization of clinical presentation, histological features, ultrasonographic findings, and survival in 29 dogs with granulomatous hepatitis.

Author

Prentice, Kayla D., Callahan‐Clark, Julie E., Parry, Nicola M., Schwarz, Leslie A., and Webster, Cynthia R. L.

Subjects
SYMPTOMS, CHRONIC active hepatitis, DOGS, CLINICAL pathology, HEPATITIS, MAST cell tumors
Abstract

Background: Granulomatous hepatitis (GH) is a form of chronic hepatitis (CH) in dogs for which limited information is published. Hypothesis: Describe the clinical presentation, clinical pathology, ultrasound, and hepatic histopathology findings and to report survival times in dogs with GH. Animals: Twenty‐nine client‐owned dogs with GH. Methods: Retrospective observational study. Pathology records were searched. Inclusion criteria included a histopathologic diagnosis of GH, absence of an identified etiology or evidence of extrahepatic granulomatous disease, and a medical record available for review. Clinical presentation, clinical pathologic findings, treatment protocols, and survival times were recorded. Available hepatic biopsy material was graded and scored, and ultrasound evaluations reviewed. Results: The median age was 7 years (range, 0.66‐12 years). Nineteen breeds were represented. Decreased appetite (19/29), lethargy (16/29), and fever (13/29) were seen most commonly. All dogs had increased serum transaminase activities, whereas 21/29 and 12/24 had hyperbilirubinemia and neutrophilia, respectively. Ultrasonographic findings included hepatomegaly (12/22), nodular parenchymal lesions (9/22), and hyperechoic parenchymal bands (8/22). Histopathologic necroinflammatory scores were moderate to severe in 16/19 dogs, and fibrosis scores were mild in 14/19 dogs. Treatments varied and included antibiotics, immunosuppressive drugs, and hepatoprotectants. Overall median survival was 635 days (range, 1‐2482 days). Conclusion and Clinical Importance: Granulomatous hepatitis in dogs is associated with high histopathologic grade, fever, neutrophilia, and a high incidence of hepatomegaly and focal parenchymal lesions on ultrasound examination. Despite disease severity on presentation, dogs with GH can have a good outcome with prolonged survival. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Multi-Omics Characterization of Inflammatory Bowel Disease-Induced Hyperplasia/Dysplasia in the Rag2−/−/Il10−/− Mouse Model

Author

Han, Qiyuan, Kono, Thomas J. Y., Knutson, Charles G., Parry, Nicola M., Seiler, Christopher L., Fox, James G., Tannenbaum, Steven R., Tretyakova, Natalia Y., Han, Qiyuan, Kono, Thomas J. Y., Knutson, Charles G., Parry, Nicola M., Seiler, Christopher L., Fox, James G., Tannenbaum, Steven R., and Tretyakova, Natalia Y.

Abstract

Epigenetic dysregulation is hypothesized to play a role in the observed association between inflammatory bowel disease (IBD) and colon tumor development. In the present work, DNA methylome, hydroxymethylome, and transcriptome analyses were conducted in proximal colon tissues harvested from the Helicobacter hepaticus (H. hepaticus)-infected murine model of IBD. Reduced representation bisulfite sequencing (RRBS) and oxidative RRBS (oxRRBS) analyses identified 1606 differentially methylated regions (DMR) and 3011 differentially hydroxymethylated regions (DhMR). These DMR/DhMR overlapped with genes that are associated with gastrointestinal disease, inflammatory disease, and cancer. RNA-seq revealed pronounced expression changes of a number of genes associated with inflammation and cancer. Several genes including Duox2, Tgm2, Cdhr5, and Hk2 exhibited changes in both DNA methylation/hydroxymethylation and gene expression levels. Overall, our results suggest that chronic inflammation triggers changes in methylation and hydroxymethylation patterns in the genome, altering the expression of key tumorigenesis genes and potentially contributing to the initiation of colorectal cancer.

Published
2022

7. Multi-Omics Characterization of Inflammatory Bowel Disease-Induced Hyperplasia/Dysplasia in the Rag2−/−/Il10−/− Mouse Model

Author

Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Division of Comparative Medicine, Han, Qiyuan, Kono, Thomas JY, Knutson, Charles G, Parry, Nicola M, Seiler, Christopher L, Fox, James G, Tannenbaum, Steven R, Tretyakova, Natalia Y, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Division of Comparative Medicine, Han, Qiyuan, Kono, Thomas JY, Knutson, Charles G, Parry, Nicola M, Seiler, Christopher L, Fox, James G, Tannenbaum, Steven R, and Tretyakova, Natalia Y

Abstract

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. Epigenetic dysregulation is hypothesized to play a role in the observed association between inflammatory bowel disease (IBD) and colon tumor development. In the present work, DNA methylome, hydroxymethylome, and transcriptome analyses were conducted in proximal colon tissues harvested from the Helicobacter hepaticus (H. hepaticus)-infected murine model of IBD. Reduced representation bisulfite sequencing (RRBS) and oxidative RRBS (oxRRBS) analyses identified 1606 differentially methylated regions (DMR) and 3011 differentially hydroxymethylated regions (DhMR). These DMR/DhMR overlapped with genes that are associated with gastrointestinal disease, inflammatory disease, and cancer. RNA-seq revealed pronounced expression changes of a number of genes associated with inflammation and cancer. Several genes including Duox2, Tgm2, Cdhr5, and Hk2 exhibited changes in both DNA methylation/hydroxymethylation and gene expression levels. Overall, our results suggest that chronic inflammation triggers changes in methylation and hydroxymethylation patterns in the genome, altering the expression of key tumorigenesis genes and potentially contributing to the initiation of colorectal cancer.

Published
2022

10. Multi-Omics Characterization of Inflammatory Bowel Disease-Induced Hyperplasia/Dysplasia in the Rag2−/−/Il10−/− Mouse Model

Author

Han, Qiyuan, Kono, Thomas J. Y., Knutson, Charles G., Parry, Nicola M., Seiler, Christopher L., Fox, James G., Tannenbaum, Steven R., Tretyakova, Natalia Y., Han, Qiyuan, Kono, Thomas J. Y., Knutson, Charles G., Parry, Nicola M., Seiler, Christopher L., Fox, James G., Tannenbaum, Steven R., and Tretyakova, Natalia Y.

Abstract

Epigenetic dysregulation is hypothesized to play a role in the observed association between inflammatory bowel disease (IBD) and colon tumor development. In the present work, DNA methylome, hydroxymethylome, and transcriptome analyses were conducted in proximal colon tissues harvested from the Helicobacter hepaticus (H. hepaticus)-infected murine model of IBD. Reduced representation bisulfite sequencing (RRBS) and oxidative RRBS (oxRRBS) analyses identified 1606 differentially methylated regions (DMR) and 3011 differentially hydroxymethylated regions (DhMR). These DMR/DhMR overlapped with genes that are associated with gastrointestinal disease, inflammatory disease, and cancer. RNA-seq revealed pronounced expression changes of a number of genes associated with inflammation and cancer. Several genes including Duox2, Tgm2, Cdhr5, and Hk2 exhibited changes in both DNA methylation/hydroxymethylation and gene expression levels. Overall, our results suggest that chronic inflammation triggers changes in methylation and hydroxymethylation patterns in the genome, altering the expression of key tumorigenesis genes and potentially contributing to the initiation of colorectal cancer.

Published
2021

18. Gut Microbiome Phenotypes Driven by Host Genetics Affect Arsenic Metabolism

Author

Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Chemistry, Massachusetts Institute of Technology. Division of Comparative Medicine, Lu, Kun, Parry, Nicola M. A., Wishnok, John S., Fox, James G., Tannenbaum, Steven Robert, Mahbub, Ridwan, Cable, Peter Hans, Ru, Hongyu, Bodnar, Wanda M., Styblo, Miroslav, Swenberg, James A., Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Chemistry, Massachusetts Institute of Technology. Division of Comparative Medicine, Lu, Kun, Parry, Nicola M. A., Wishnok, John S., Fox, James G., Tannenbaum, Steven Robert, Mahbub, Ridwan, Cable, Peter Hans, Ru, Hongyu, Bodnar, Wanda M., Styblo, Miroslav, and Swenberg, James A.

Abstract

Large individual differences in susceptibility to arsenic-induced diseases are well-documented and frequently associated with different patterns of arsenic metabolism. In this context, the role of the gut microbiome in directly metabolizing arsenic and triggering systemic responses in diverse organs raises the possibility that gut microbiome phenotypes affect the spectrum of metabolized arsenic species. However, it remains unclear how host genetics and the gut microbiome interact to affect the biotransformation of arsenic. Using an integrated approach combining 16S rRNA gene sequencing and HPLC-ICP-MS arsenic speciation, we demonstrate that IL-10 gene knockout leads to a significant taxonomic change of the gut microbiome, which in turn substantially affects arsenic metabolism., National Institute of Environmental Health Sciences (P30 ES010126), National Institute of Environmental Health Sciences (NIEHS grant P30 ES002109), University of Georgia. College of Public Health (internal grant), University of Georgia (Faculty Research Grant (FRG))

Published
2015

19. In Vivo Biosensing Via Tissue-Localizable Near-Infrared-Fluorescent Single-Walled Carbon Nanotubes

Author

Iverson, Nicole M., Barone, Paul W., Shandell, Mia, Trudel, Laura J., Sen, Selda, Sen, Fatih, Ivanov, Vsevolod, Atolia, Esha, Farias, Edgardo, McNicholas, Thomas P., Reuel, Nigel, Parry, Nicola M. A., Wogan, Gerald N., and Strano, Michael S.

Abstract

Single-walled carbon nanotubes are particularly attractive for biomedical applications, because they exhibit a fluorescent signal in a spectral region where there is minimal interference from biological media. Although single-walled carbon nanotubes have been used as highly sensitive detectors for various compounds, their use as in vivo biomarkers requires the simultaneous optimization of various parameters, including biocompatibility, molecular recognition, high fluorescence quantum efficiency and signal transduction. Here we show that a polyethylene glycol ligated copolymer stabilizes near-infrared-fluorescent single-walled carbon nanotubes sensors in solution, enabling intravenous injection into mice and the selective detection of local nitric oxide concentration with a detection limit of 1 mu M. The half-life for liver retention is 4 h, with sensors clearing the lungs within 2 h after injection, thus avoiding a dominant route of in vivo nanotoxicology. After localization within the liver, it is possible to follow the transient inflammation using nitric oxide as a marker and signalling molecule. To this end, we also report a spatial-spectral imaging algorithm to deconvolute fluorescence intensity and spatial information from measurements. Finally, we demonstrate that alginate-encapsulated single-walled carbon nanotubes can function as implantable inflammation sensors for nitric oxide detection, with no intrinsic immune reactivity or other adverse response for more than 400 days.

Published
2013

22. In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes

Author

Massachusetts Institute of Technology. Center for Biomedical Innovation, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Chemical Engineering, Massachusetts Institute of Technology. Department of Mechanical Engineering, Massachusetts Institute of Technology. Division of Comparative Medicine, Iverson, Nicole M., Barone, Paul W., Shandell, Mia, Sen, Selda, Sen, Fatih, McNicholas, Thomas P., Reuel, Nigel, Strano, Michael S., Trudel, Laura J., Ivanov, Vsevolod, Atolia, Esha, Farias, Edgardo, Wogan, Gerald N., Parry, Nicola M. A., Massachusetts Institute of Technology. Center for Biomedical Innovation, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Chemical Engineering, Massachusetts Institute of Technology. Department of Mechanical Engineering, Massachusetts Institute of Technology. Division of Comparative Medicine, Iverson, Nicole M., Barone, Paul W., Shandell, Mia, Sen, Selda, Sen, Fatih, McNicholas, Thomas P., Reuel, Nigel, Strano, Michael S., Trudel, Laura J., Ivanov, Vsevolod, Atolia, Esha, Farias, Edgardo, Wogan, Gerald N., and Parry, Nicola M. A.

Abstract

Single-walled carbon nanotubes are particularly attractive for biomedical applications, because they exhibit a fluorescent signal in a spectral region where there is minimal interference from biological media. Although single-walled carbon nanotubes have been used as highly sensitive detectors for various compounds, their use as in vivo biomarkers requires the simultaneous optimization of various parameters, including biocompatibility, molecular recognition, high fluorescence quantum efficiency and signal transduction. Here we show that a polyethylene glycol ligated copolymer stabilizes near-infrared-fluorescent single-walled carbon nanotubes sensors in solution, enabling intravenous injection into mice and the selective detection of local nitric oxide concentration with a detection limit of 1 µM. The half-life for liver retention is 4 h, with sensors clearing the lungs within 2 h after injection, thus avoiding a dominant route of in vivo nanotoxicology. After localization within the liver, it is possible to follow the transient inflammation using nitric oxide as a marker and signalling molecule. To this end, we also report a spatial-spectral imaging algorithm to deconvolute fluorescence intensity and spatial information from measurements. Finally, we demonstrate that alginate-encapsulated single-walled carbon nanotubes can function as implantable inflammation sensors for nitric oxide detection, with no intrinsic immune reactivity or other adverse response for more than 400 days., National Institutes of Health (U.S.) (T32 Training Grant in Environmental Toxicology ES007020), National Cancer Institute (U.S.) (Grant P01 CA26731), National Institute of Environmental Health Sciences (Grant P30 ES002109), Arnold and Mabel Beckman Foundation (Young Investigator Award), National Science Foundation (U.S.). Presidential Early Career Award for Scientists and Engineers, Scientific and Technological Research Council of Turkey (TUBITAK 2211 Research Fellowship Programme), Scientific and Technological Research Council of Turkey (TUBITAK 2214 Research Fellowship Programme), Middle East Technical University. Faculty Development Programme, Sanofi Aventis (Firm) (Biomedical Innovation Grant)

Published
2014

28. Gut Microbiome Perturbations Induced by Bacterial Infection Affect Arsenic Biotransformation

Author

Lu, Kun, primary, Cable, Peter Hans, additional, Abo, Ryan Phillip, additional, Ru, Hongyu, additional, Graffam, Michelle E., additional, Schlieper, Katherine Ann, additional, Parry, Nicola M. A., additional, Levine, Stuart, additional, Bodnar, Wanda M., additional, Wishnok, John S., additional, Styblo, Miroslav, additional, Swenberg, James A., additional, Fox, James G., additional, and Tannenbaum, Steven R., additional

Published
2013
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29. In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes

Author

Iverson, Nicole M., primary, Barone, Paul W., additional, Shandell, Mia, additional, Trudel, Laura J., additional, Sen, Selda, additional, Sen, Fatih, additional, Ivanov, Vsevolod, additional, Atolia, Esha, additional, Farias, Edgardo, additional, McNicholas, Thomas P., additional, Reuel, Nigel, additional, Parry, Nicola M. A., additional, Wogan, Gerald N., additional, and Strano, Michael S., additional

Published
2013
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37. Persistent effect of mTOR inhibition on preneoplastic foci progression and gene expression in a rat model of hepatocellular carcinoma.

Author

Francois-Vaughan, Heather, Adebayo, Adeola O., Brilliant, Kate E., Parry, Nicola M. A., Gruppuso, Philip A., and Sanders, Jennifer A.

Subjects
LIVER cancer, PRECANCEROUS conditions, HETEROGENEOUS catalysis, PROGENITOR cells, IMMUNOSTAINING
Abstract

Hepatocellular carcinoma (HCC) is a heterogeneous disease in which tumor subtypes can be identified based on the presence of adult liver progenitor cells. Having previously identified the mTOR pathway as critical to progenitor cell proliferation in a model of liver injury, we investigated the temporal activation of mTOR signaling in a rat model of hepatic carcinogenesis. The model employed chemical carcinogens and partial hepatectomy to induce progenitor markerpositive HCC. Immunohistochemical staining for phosphorylated ribosomal protein S6 indicated robust mTOR complex 1 (mTORC1) activity in early preneoplastic lesions that peaked during the first week and waned over the subsequent 10 days. Continuous administration of rapamycin by subcutaneous pellet for 70 days markedly reduced the development of focal lesions, but resulted in activation of the PI3K signaling pathway. To test the hypothesis that early mTORC1 activation was critical to the development and progression of preneoplastic foci, we limited rapamycin administration to the 3-week period at the start of the protocol. Focal lesion burden was reduced to a degree indistinguishable from that seen with continuous administration. Short-term rapamycin did not result in the activation of PI3K or mTORC2 pathways. Microarray analysis revealed a persistent effect of short-term mTORC1 inhibition on gene expression that resulted in a genetic signature reminiscent of normal liver. We conclude that mTORC1 activation during the early stages of hepatic carcinogenesis may be critical due to the development of preneoplastic focal lesions in progenitor marker-positive HCC. mTORC1 inhibition may represent an effective chemopreventive strategy for this form of liver cancer. [ABSTRACT FROM AUTHOR]

Published
2016
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42. Treatment With 17 Beta-Estradiol Prevents the Development of Cancer in Chronically H. pylori Infected INS-GAS Mice by Shifting the Inflammatory Pathways From That of Males to One Resembling Females

Author

Sheh, Alexander, primary, Parry, Nicola M., additional, Mobley, Melissa W., additional, McCabe, Amanda F., additional, Rager, Julia, additional, Racynski, Arek R., additional, Fry, Rebecca C., additional, Wang, Timothy C., additional, and Fox, James G., additional

Published
2011
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50. Mast cell tumor invading the cornea in a horse.

Author

Halse, Stacey, Pizzirani, Stefano, Parry, Nicola M. A., and Burgess, Kristine E.

Subjects
MAST cell tumors, CORNEA, CANCER invasiveness, EYELIDS, CONJUNCTIVA, HISTOPATHOLOGY, HORSE diseases, TUMORS
Abstract

A 3-year-old Marwari mare was presented for evaluation of an irregular, reddish mass protruding from behind the right third eyelid. The mass appeared to arise at the ventral limbal area, involved the perilimbal bulbar conjunctiva and widely extended into corneal tissue. No other ocular or systemic abnormalities were detected at the time of presentation. The mass was surgically removed by lamellar keratectomy, with defocused CO2 laser used as adjunctive therapy to treat the surgical exposed area and its surroundings. Histopathologic evaluation showed sheets of densely packed, well-differentiated neoplastic mast cells separated by fibrovascular connective tissue. Nuclear staining for Ki-67 was performed, and an average of 370 cells were positive per 1000 counted cells. Two months postoperatively, the surgical site was filled with flat fibrovascular and pigmented tissue, while the surrounding cornea was transparent with no superficial vascularization around the fibrotic scar. Thirty-two months after treatment, no recurrence of the neoplasia was reported. [ABSTRACT FROM AUTHOR]

Published
2014
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