1. Further evidence for the existence of endogenous serotonin uptake inhibitors and their purification by calmodulin affinity chromatography.
- Author
-
Chudzik J, Strijewski A, and Tang SW
- Subjects
- Adult, Antibodies analysis, Biological Factors analysis, Brain metabolism, Calmodulin metabolism, Chromatography, Agarose, Chromatography, Gel, Depressive Disorder drug therapy, Depressive Disorder immunology, Depressive Disorder metabolism, Female, Humans, Imipramine immunology, Imipramine metabolism, Imipramine therapeutic use, Male, Paroxetine immunology, Paroxetine metabolism, Paroxetine therapeutic use, Radioimmunoassay, Serotonin immunology, Serotonin therapeutic use, Structure-Activity Relationship, Biological Factors metabolism, Serotonin metabolism, Selective Serotonin Reuptake Inhibitors
- Abstract
Experimental evidence suggests the occurrence of endogenous antidepressant-like compounds in the brain, blood plasma, and urine. After extensive purification by calmodulin-sepharose affinity chromatography, and further purification of the urine-derived sample by exclusion chromatography, at least three distinctive fractions have been identified. These fractions effectively inhibited serotonin uptake, imipramine, and/or paroxetine binding, and they apparently contained some compounds that were recognized by the anti-imipramine or anti-paroxetine antibodies. Their identification may have significant implications for studies of affective illness.
- Published
- 1993
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