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Objective The aim of this study was to identify the ideal anchor point for patellar anti-rotational sutures for adjunctive stabilization of medial patellar luxation in both small and large breed dogs. Study Design Retrospective radiographic survey was performed on 110 stifles from 101 dogs. Radiographs were grouped based on patient weight (≤15 kg; >15 kg) and diagnosis (medial patellar luxation, cranial cruciate ligament rupture, and normal joints). Radiographic measurements included: the proximal, middle, and distal points of the trochlear ridge, the caudal aspect of Blumensaat's line (roof of the intercondylar notch), the centre of the lateral fabella, as well as the “best-fit” centre of a circle overlying the trochlea. These landmark coordinates were used to calculate radii for comparison, and for scaling between joints. Results Use of the fabellar centre resulted in larger radii (corrected p Conclusion The fabella is unlikely to be the best choice for anchoring a patellar anti-rotational suture. Use of the best-fit circle centre to place a suture anchor should be preferred to maximise suture isometry during joint flexion and extension in large and small breed dogs.

OBJECTIVE: To evaluate the effect of center of rotation of angulation (CORA)-based leveling osteotomy (CBLO) and hamstring load on stifle stability following cranial cruciate ligament transection (CCLx) and medial meniscal release (MMR).STUDY DESIGN: Ex vivo experimental study.SAMPLE POPULATION: Cadaver hind limb preparations (n = 7).METHODS: After instrumentation, constant quadriceps and gastrocnemius loads with an optional hamstring load in a 3:1:0.6 ratio were applied, and stifles were extended from fully flexed using an electrical motor during fluoroscopic recording. The recording process was repeated after each of CCLx, MMR and CBLO and the extracted landmark coordinates were used for calculation of cranial tibial translation (CTT) and patellar ligament angle (PTA).RESULTS: Mean initial tibial plateau angle was 28.1°: post-CBLO the mean was 9.7°. Cranial tibial translation developed from 50° and 75° with CCLx and MMR respectively (p < .04, < .02) without hamstring loading. Hamstring loading mitigated CTT due to CCLx and delayed CTT until 120° for MMR (P < .02) in this model. CBLO prevented CTT, except at 140° without hamstring loading (P = .01). Similar results were seen for PTA, but CBLO curves were parallel to and lower than intact values at all tested angles (P < .04), consistent with induced effective joint flexion.CONCLUSION: CBLO to a target tibial plateau angle of 10° largely eliminated CTT induced by CCLx and MMR. Hamstring loads of 20% quadriceps load improved stifle stability in this model.IMPACT: Stifle stability following CBLO appears to be multifactorial and depends on meniscal integrity, joint angle, and hamstring strength.

Objectives: The aim of this study was to evaluate neurobehavioral toxicity of single-walled (SWNTs) and multiwalled carbon nanotubes (MWNTs) in mice. Methods: Male NMRI mice were randomized into 5 groups ( n = 10 each): Normal control (NC) group was injected intraperitoneally (i.p.) with phosphate-buffered saline (PBS) solution (pH 7.8; ca. 1 mL), MW80 and MW800 groups were injected with either i.p. 80 or 800 mg kg−1 MWNTs suspended in 1 mL of PBS and SW80 and SW800 groups were injected with either i.p. 80 or 800 mg kg−1 SWNTs suspended in 1 mL of PBS. After 2 weeks, five mice from each group were evaluated for brain-derived neurotrophic factor (BDNF) messenger RNA expression and protein content of brain tissues. Locomotion, anxiety, learning and memory, and depression were measured by open field test (OFT), elevated plus-maze (EPM), object recognition test (ORT), and forced swimming test (FST), respectively. Results: Ambulation time and center arena time in the OFT did not change among groups. In the EPM paradigm, SWNTs (800 mg kg−1) and MWNTs (80 and 800 mg kg−1) showed an anxiogenic effect. In ORT, MWNTs (80 mg kg−1) increased the discrimination ratio while in FST, MWNTs showed a depressant effect as compared to vehicle. The BDNF gene expression in mice treated with 80 and 800 mg kg−1 SWNTs or 80 mg kg−1 MWNTs decreased as compared to NC mice although BDNF gene expression increased in mice that were treated with 800 mg kg−1 MWNTs. The whole brain BDNF protein content did not change among groups. Conclusion: Our study showed that i.p. exposure to carbon nanotubes (CNTs) may result in behavioral toxicity linked with expression of depression or anxiety that depends on the type of CNTs. In addition, exposure to CNTs changed BDNF gene expression.

The aim of this study was to evaluate neurobehavioral toxicity of single-walled (SWNTs) and multiwalled carbon nanotubes (MWNTs) in mice.Male NMRI mice were randomized into 5 groups ( n = 10 each): Normal control (NC) group was injected intraperitoneally (i.p.) with phosphate-buffered saline (PBS) solution (pH 7.8; ca. 1 mL), MW80 and MW800 groups were injected with either i.p. 80 or 800 mg kgAmbulation time and center arena time in the OFT did not change among groups. In the EPM paradigm, SWNTs (800 mg kgOur study showed that i.p. exposure to carbon nanotubes (CNTs) may result in behavioral toxicity linked with expression of depression or anxiety that depends on the type of CNTs. In addition, exposure to CNTs changed BDNF gene expression.