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15 results on '"Pardini, Alessandra"'

1. HOW AFRICA CAN LEAD THE GLOBAL RACE FOR GREEN HYDROGEN PRODUCTION.

Author

Scales, Tim, Edwards, Troy, Clüver, Alexandra, Haddad, Antoine, and Pardini, Alessandra

Subjects
HYDROGEN production, JOB creation, INVESTMENTS
Abstract

The article focuses on Africa's potential as a future global hub for green hydrogen production, highlighting several major regional projects in countries like Morocco, Mauritania, Djibouti, Namibia, and South Africa. It emphasizes the significant investment opportunities and potential for the continent to secure a substantial share of the global hydrogen market, projecting substantial economic benefits and job creation.

Published
2024

2. Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

Author

BRAGAZZA Lúcia M., VAZ Adelaide J., PASSOS Afonso D.C., TAKAYANAGUI Osvaldo M., NAKAMURA Paulo M., ESPÍNDOLA Noeli M., PARDINI Alessandra, and BUENO Ednéia C.

Subjects
Cysticercosis, Seroprevalence, Immunologic tests, Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeirão Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1%.

Published
2002

4. Taenia antigens detection in the cerebrospinal fluid of patients with neurocysticercosis and its relationship with clinical activity of the disease

Author

Abraham, Ronaldo, Pardini, Alessandra Xavier, Vaz, Adelaide José, Livramento, José Antonio, and Machado, Luís dos Ramos

Subjects
neurocysticercosis clinical activity, atividade clínica da neurocisticercose, parasitic diseases, neurocysticercosis, Taenia antigens, neurocisticercose, antígenos de Taenia
Abstract

OBJECTIVE: (1) To determine the concentration of Taenia antigens in the cerebrospinal fluid (CSF) of patients with neurocysticercosis (NC); (2) to establish its relationship with clinical activity of the disease and with classical variables of CSF. METHOD: A CSF examination was performed in one sample from 36 patients with definitive diagnosis of NC, including: quantitative and cytomorphological study, biochemical tests, immunological reactions for cysticercosis and Taenia antigens. The antibodies for antigens detection were obtained from the larval form of Taenia crassiceps, ORF strain. After intraperitoneal passage through female mice, a group of rabbits was immunized with vesicular fluid antigens. RESULTS: The Taenia antigen was detected in CSF from 17 patients (47.2%), especially in those patients with epileptic symptoms in the last 12 months. CONCLUSION: Taenia antigens presence in CSF have significant relationship with clinically active forms of NC, being a more sensitive marker than the classic eosinophil presence. OBJETIVO: (1) Determinar a concentração de antígenos de Taenia no líquido cefalorraquidiano (LCR) em doentes com neurocisticercose; (2) estudar sua relação com a atividade clínica da doença e com as variáveis clássicas do LCR. MÉTODO: Em 36 pacientes com diagnóstico definido de neurocisticercose foi realizado exame do LCR, com estudo citológico e citomorfológico, exame bioquímico, reações imunológicas para cisticercose e detecção de antígenos de Taenia. Os anticorpos para detecção desses antígenos foram obtidos a partir da forma larvar da Taenia crassiceps, cepa ORF. Após a inoculação e proliferação intraperitoneal dessa forma larvária em ratas, foi imunizado um grupo de coelhos com seu líquido vesicular. RESULTADOS: Em 17 pacientes (47,2%) foi detectado antígeno de Taenia, especialmente naqueles com manifestação epiléptica nos últimos 12 meses. CONCLUSÃO: A detecção de antígeno de Taenia guarda relação significativa com a vigência de formas clinicamente ativas, sendo, nestas formas, marcador mais sensível que a eosinofilorraquia.

Published
2004

14. Use of Taenia crassicepsCysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium)

Author

Pardini, Alessandra Xavier, Peralta, Regina Helena, Vaz, Adelaide Jose´, Machado, Luis dos Ramos, and Peralta, Jose´ Mauro

Abstract

ABSTRACTAntigen extracts obtained from the vesicular fluid of Taenia crassicepscysticerci and from fractions purified by affinity chromatography with the lectin concanavalin A and the glycoprotein antigen separated by electrophoresis were used for the detection of Taenia soliumanticysticercus antibodies. The sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. Using immunoblotting of the three antigens, low-molecular-mass peptides (18 and 14 kDa) were characterized only in cerebrospinal fluid samples from patients with neurocysticercosis. The results confirm that antigen fractions purified from T. crassicepscisticerci are important sources of specific peptides and proved to be efficient in detecting anti-T. soliumantibodies.

Published
2002
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15. Use of Taenia crassiceps cysticercus antigen preparations for detection of antibodies in cerebrospinal fluid samples from patients with neurocysticercosis (Taenia solium).

Author

Pardini AX, Peralta RH, Vaz AJ, Machado Ldos R, and Peralta JM

Subjects
Animals, Enzyme-Linked Immunosorbent Assay, Humans, Immunoblotting, Neurocysticercosis cerebrospinal fluid, Antibodies, Helminth cerebrospinal fluid, Antigens, Helminth immunology, Neurocysticercosis immunology, Taenia immunology
Abstract

Antigen extracts obtained from the vesicular fluid of Taenia crassiceps cysticerci and from fractions purified by affinity chromatography with the lectin concanavalin A and the glycoprotein antigen separated by electrophoresis were used for the detection of Taenia solium anticysticercus antibodies. The sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. Using immunoblotting of the three antigens, low-molecular-mass peptides (18 and 14 kDa) were characterized only in cerebrospinal fluid samples from patients with neurocysticercosis. The results confirm that antigen fractions purified from T. crassiceps cisticerci are important sources of specific peptides and proved to be efficient in detecting anti-T. solium antibodies.

Published
2002
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