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Yogurt is a functional drink that has been known to have health benefits. Yogurt has higher antioxidant activity than whole milk. This study aimed to determine the ability of antioxidant and antibacterial activity of Songi fruit extract yogurt. The antioxidant activity was assayed using DPPH (1,1-diphenyl-picrylhydrazyl) and the antibacterial activity using the agar diffusion method with the well-technique against Gram-negative bacteria Escherichia coli ATCC 35218. The test results of secondary metabolites of Songi fruit extract contain tannins, saponins, and flavonoids. The Songi fruit extract was formulated into 4 formulas, which were Formula I (0%), Formula II (10%), Formula III (15%), and Formula IV (20%). The results of the antioxidant activity test obtained with IC50 values of formulas were 121, 75.38, 50.50, and 36.58 mg/mL respectively, and 84.82 mg/mL for commercial yogurt. Test results of antibacterial activity against bacteria E. coli ATCC 35218 with inhibition zone diameters were 0, 0, 1.5, and 3.5 mm, respectively for Formula I-IV, and the commercial yogurt was 0 mm. Formula IV (20% fruit extract yogurt) possessed the highest antioxidant and antibacterial activity with IC50 36.58 mg/mL and an inhibition zone diameter of 3.5 mm. In conclusion, the Songi fruit extract yogurt has antioxidant and antibacterial activity against E. coli.

Gedi Merah leaf (Abelmoschus manihot L.Medik) is a tropical plant used by the community as a traditional medicine to reduce cholesterol, hypertension, and antidiabetic levels. Diabetes mellitus is a disorder of carbohydrate, protein, and fat metabolism due to a lack of the hormone insulin so that glucose in the blood increases which is characterized by changes in the histopathological structure of the pancreatic islets of Langerhans. The purpose of this study was to see the histopathological description of the pancreas in male white rats (Rattus norvegicus) Wistar strain model of type II diabetes mellitus by administering ethanol extract of red gedi leaves (Abelmoschus manihot L.Medik). Modeling of diabetes mellitus in experimental animals was carried out by administering a high-fat diet and intraperitoneal induction of streptozotocin 30 mg/kg. After modeling, male rats were grouped into 6 treatment groups, namely a normal control group, a positive control group, a negative control group, and a group that was given the ethanol extract of red gedi leaves at a dose of 75 mg/kg BW, 150 mg/kg BW 300 mg/kg BW. The results of the histopathological examination of the pancreas and the number of endocrine cells showed that the ethanolic extract of red gedi leaves (Abelmoschus manihot L.Medik) was effective in repairing pancreatic cells and regenerating endocrine cells in the islets of langerhans.

Bitter melon (Momordica charantia L.) is one of the plants that had been widely known and widely used by the society as a daily dish and as a traditional medicine to treat various diseases. This plant contains alkaloids, triterpenoids, saponins, and flavonoids are thought to be toxic to some degree. The aims of the study is to determine the effect of ethanol extract of bitter melon on the toxicity value and histopatology representation of mencit liver system. This type of research is quasi experimental with post test only control group design. The subjects of this study were male mice (mus musculus) is divided into 5 groups of group I treated with bitter melon extract of 5 mg / kgBB, group II was given bitter melon extract 50 mg / kgBB, group III was given bitter melon extract 500 mg / kgBB, IV was given 5000 mg / kgBB, and group V was given aquadest as a negative control. The treatment was performed for 14 days and a day afterwards microscopic examination of liver system in mice using light microscope. The toxicity effect test using fixed dose method as the method.The acute toxicity test of the ethanol extract of the pair (Momordica charantial L.) on LD50 and the results of histopathology in the necrosis of group 1 zonal, group necrosis of groups 2 and 3 and necrosis of group 4 of the bridge for 24 hours with 16 mice die in each treatment group. Conclusion of the LD50 test for 75% of the mortality in group 4, the extracts of the pair had toxic activity with non-toxic practical criteria, and the histology of the liver of the mice showed toxicological activity with the result of the test at a dose of 5000 mg / kgBB, in all parts of the mouse liver was necrosis

A booster vaccination is a COVID-19 vaccination after a person has received a complete primary dose of vaccination aimed at maintaining the level of immunity and extending the period of protection. The results of the study show that there has been a decrease in antibodies at six months after receiving a complete primary dose of COVID-19 vaccination, so that a follow-up or booster dose is needed to increase individual protection, especially in vulnerable groups of people. This Community Service activity was held on Monday, March 14, 2022 at 08.00 WITA at the Faculty of Earth Science and Technology (FITK) Halu Oleo University Kendari, which was attended by the Dean of the Faculty of Earth Science and Technology (FITK) Halu Oleo University, the Covid Vaccination Team -19 consisting of doctors, nurses and IT officers, and the Academic Community of the Faculty of Earth Science and Technology (FITK) as vaccination participants. The target of this Community Service activity is the Academic Community of the Faculty of Earth Science and Technology (FITK) at Halu Oleo Kendari University as many as 27 people, who were declared healthy and eligible to receive the Covid-19 vaccination after passing the registration and screening process from doctors. Abstrak Vaksinasi booster adalah vaksinasi COVID-19 setelah seseorang mendapat vaksinasi primer dosis lengkap yang ditujukan untuk mempertahankan tingkat kekebalan serta memperpanjang masa perlindungan. Hasil studi menunjukkan telah terjadi penurunan antibodi pada enam bulan setelah mendapatkan vaksinasi COVID-19 dosis primer lengkap, sehingga dibutuhkan pemberian dosis lanjutan atau booster untuk meningkatkan proteksi individu terutama pada kelompok masyarakat rentan. Kegiatan Pengabdian Kepada Masyarakat ini dilaksanakan pada hari senin, Tanggal 14 Maret 2022 Jam 08.00 Wita di Fakultas Ilmu dan Teknologi Kebumian (FITK) Universitas Halu Oleo Kendari, yang dihadiri oleh Dekan Fakultas Ilmu dan Teknologi Kebumian (FITK) Universitras Halu Oleo, Tim Vaksinasi Covid-19 yang terdiri dari dokter, perawat dan petugas IT, dan Civitas Akademika Fakultas Ilmu dan Teknologi Kebumian (FITK) sebagai peserta vaksinasi. Sasaran kegiatan Pengabdian Kepada Masyarakat ini adalah Civitas Akademika Fakultas Ilmu dan Teknologi Kebumian (FITK) dilingkup Universitas Halu Oleo Kendari sebanyak 27 orang, yang dinyatakan sehat dan layak untuk mendapatkan vaksinasi Covid-19 setelah melewati proses registrasi dan screening dari dokter.

Prevalence of diabetes mellitus according to WHO been projected increase from 2.8% in 2000 to 4.4% in 2030. Various studies have been conducted to find alternative sources of antidiabetic therapy, Momordica charantia known has mimetic insulin compounds. Ethanol extract of Momordica charantia reported show optimal results in reducing blood glucose levels and stimulating pancreatic beta cells in secreting insulin hormone. This study aims to determine effect of bitter melon extract on insulin levels in streptozotosin-induced rat. This study consisted in 3 groups test namely streptozotosin-induced rat, streptozotosin-induced rat given ethyl acetate fraction of Momordica charantia extract, and not induced-streptozotosin rats. Insulin levels measured by ELISA method. Data analysis used Unpaired T-test considered if (p value >0,05). Research result show that there is not different between the insulin levels of streptozotosin-induced rat with streptozotosin-induced rat given ethyl acetate fraction of Momordica charantia extract group (p=0,081). There is not different between insulin levels of streptozotosin-induced rat given ethyl acetate fraction of Momordica charantia extract with not induced-streptozotosin rats (p=0,505). Conclusion. There is not different between insulin levels of streptozotosin-induced rat, streptozotosin-induced rat given ethyl acetate fraction of Momordica charantia extract and not induced streptozotosin rats group.

A toxicity test is a test to detect the toxic effect of a substance on a biological system and to obtain typical dose-response data from the test preparation. This study aims to determine the content of secondary metabolites from the aqueous extract and ethanolic extract of gardenia leaves, characterization of gardenia leaf extract, and acute toxicity using the BSLT method against Artemia salina Leach shrimp larvae.Phytochemical screening tests were carried out by the color change method which included the alkaloid test, flavonoid test, tannin test, saponin test, and terpenoid test. Extract characterization tests include determination of water-soluble extract, determination of ethanol-soluble extract, determination of water content, and determination of ash content. Acute toxicity test using the Brine Shrimp Lethality Test (BSLT) method using Artemia salina Leach shrimp larvae.The results of the phytochemical screening test for the aqueous extract of gardenia leaves showed positive results on saponins, while the phytochemical screening tests on ethanol extracts showed positive results on alkaloids, flavonoids, saponins, tannins, and terpenoids. The results of the characterization test on the aqueous extract of gardenia leaves were that the ethanol-soluble extract content was 20.19%, the water-soluble extract content was 23.17%, the water content was 99.13% and the ash content was 0.29%. Meanwhile, in the ethanolic extract of gardenia leaves, the ethanol-soluble extract content was 60.80%, the water-soluble extract content was 57.14%, the water content was 7.87% and the ash content was 3.22%. The results show that gardenia leaves are not toxic with the LC50 value of the water extract of the gardenia leaves is 1399.64 g/mL and the LC50 value of the ethanolic extract of the gardenia leaves is 1080.96 so that it can be used as traditional medicine.

Stunting dapat memberikan dampak bagi kelangsungan hidup anak. WHO membagi dampak yang diakibatkan oleh stunting menjadi dua yang terdiri dari jangka pendek dan jangka panjang. Dampak jangka pendek dari stunting adalah di bidang kesehatan yang dapat menyebabkan peningkatan mortalitas dan morbiditas. Faktor keluarga dan rumah tangga dibagi lagi menjadi faktor maternal dan faktor lingkungan rumah. Hasil, kualitas makanan yang rendah dapat berupa kualitas mikronutrien yang rendah, keragaman jenis makanan yang dikonsumsi dan sumber makanan hewani yang rendah, makanan yang tidak mengandung nutrisi, dan makanan komplementer yang mengandung energi rendah. Penggunaan air bersih dan air minum yang dimasak, serta semua kepala keluarga telah memiliki tempat Buang Air Besar, serta selalu menjaga kebersihan badan dan mencuci tangan sebelum makan dan tidur. Kesimpulan, Peserta diikuti oleh kader posyandu, ketua RT/RW, dan ibu penggerak PKK. Peningkatan kesadaran masyarakat dari faktor resiko Stunting sudah cukup baik yang dapat dilihat pada profil Puskesmas Benu-Benua dengan menempatkan penyakit diare pada urutan 13.

BACKGROUND: Chronic hyperglycemia causes an increase of free radical production and in longterm, the hyperglycemia increases oxidative stress. Among medicinal plants, Momordica charantia L. fruit has been known to overcome hyperglycemia. However, role of M. charantia L. fruit on oxidative stress is not well understood. Therefore, current study was conducted to investigate the effect of M. charantia L. fruit extract on malondialdehyde (MDA) level and hepatic damage in hyperglicemic rat model. METHODS: Twenty five white rats (Rattus novergicus) were induced with Streptozotocin (STZ) and treated with/without glibenclamide, sodium carboxymethyl cellulose (Na-CMC), or M. charantia L. fruit ethanol/ethyl acetate/ n-hexane fraction. After the treatment, rat’s livers were collected and separated for histopathological examination and MDA analysis. RESULTS: The MDA level average of rats before the STZ induction was 1.37 μg/mL. MDA level average was markedly increased (23.85 μg/mL) in rats induced with STZ and treated with Na-CMC merely. The MDA level average of STZ-induced glibenclamide-treated rats was 3.12 μg/mL. Meanwhile, the MDA level averages of STZ-induced M. charantia L. fruit ethanol, ethyl acetate and n-hexane fractions-treated rats were 14.95, 8.98 and 5.37 μg/mL, respectively. The histopathology results of this study showed that adipocytes, dilated sinusoids and central vein thickening were mostly observed in STZ-induced Na-CMC-treated rats. Meanwhile, the STZ-induced ethanol/ethyl acetate/n-hexane fraction-treated rats did not exhibitthose expressions. CONCLUSION: M. charantia L. fruit fractions inhibit the MDA level average in liver tissue and regenerate hepatic damage of STZ-induced rats, especially the n-hexane fraction which could be a potential hepatic antioxidant and regenerative agent. KEYWORDS: Momordica charantia L., malondialdehyde, oxidative stress, hyperglycemia, diabetes mellitus

This study aimed to determine the role of component 5A on visitor satisfaction Celosia flower garden. The population in the study are all visitors to the celosia flower garden in 2019-2021. The sampling technique used is simple random sampling of 100 people. From the research results show the results of the regression equation Y=19.483+7.181X, namely component 5A has a positive and significant impact on visitor satisfaction with a significant value of 0.000

Diabetes Mellitus is a chronic metabolic disorder characterized by high blood glucose levels (hyperglycemia) due to the pancreas not producing enough insulin or the body cannot use the insulin it produces effectively. Gardenia leaves (Gardenia jasminoides Ellis) contain natural hydrocolloids which have a hypoglycemic effect. The designs used were pre-and post-test with control group design. 24 rats were divided into 6 groups. The negative control group was given 0.5% CMC-Na, the positive control group was given 5 mg glibenclamide, and the three treatment groups were given an extract of gardenia leaves (G. jasminoides Ellis.) with doses of 1.25 g/kg BW, 2.5 g/kg BW, and 5 g/kg BW. Rats were induced by 40 mg/kg BW streptozotocin intraperitoneally, and then 24 hours after induction, blood glucose levels were measured. Then the treatment was carried out in each group for 7 days. After that, blood glucose levels were measured using a spectrophotometer. The results of phytochemical screening of gardenia leaves were positive for saponins and pectin. The average difference in blood glucose level changes in each group was 440 mg/dL for the positive control group, 47 mg/dL for the negative control group, 284 mg/dL for the 1.25 g/kg BW-dose group, 356 mg/dL for the 2.5 g/kg BW dose-group, 313 mg/dL for the 5 g/kg BW-dose group, and -14 mg/dL for the normal group. The conclusion of this study is that the extract of gardenia leaves (G. jasminoides Elis.) has an anti-hyperglycemic effect on blood glucose levels of male Wistar rats with type II DM (Sig.

Lansia adalah seseorang yang telah berusia di atas 60 tahun dan tidak berdaya mencari nafkah sendiri untuk memenuhi kebutuhan hidupnya sehari-hari (Ratnawati, 2017). Usia lanjut ditandai dengan penurunan produktivitas kerja dan dihadapkan pada berbagai kebutuhan yang semakin meningkat seperti kebutuhan akan makanan yang bergizi seimbang, pemeriksaan kesehatan secara rutin, kebutuhan sosial, rekreasi dan menjadi tanggungan anggota keluarga (Suardiman, 2011). Memberdayakan wanita lansia merupakan salah satu upaya membantu mereka memiliki aktivitas yang dapat memunculkan peluang usaha yang ringan mereka kerjakan, seperti memanfaatkan limbah kain perca menjadi produk berupa keset. Metode yang digunakan adalah metode pendekatan emosional dalam bentuk dampingan secara langsung di lapangan, dengan itu pelatihan pemanfaatan limbah kain perca dilaksanakan di sekretariat karang taruna sabanusa Desa Arjosari.. Dalam hal ini dimulai dengan survei lapangan, sosialisasi program, pemberian motivasi, demonstrasi dan tutorial pembuatan produk, praktek dan evaluasi. Pelatihan pemanfaatan limbah kain perca bermanfaat untuk memberdayakan wanita lansia demi mengoptimalkan kain perca menjadi kerajinan tangan yang bernilai ekonomi serta meningkatkan pengetahuan, keterampilan, sikap dan peluang usaha

Antiseptic is a chemical substance whose mechanism of action is to destroy microorganisms or inhibit its work so that it can prevent infection. One of the antiseptics is chlorhexidine and alcohol. The method used in this service is the comparative method of several journal sources. A literature study is conducted online by searching for journals. The results of this dedication show that the use of antiseptics and disinfectants to prevent Covid-19 transmission is effective if the selection is right and used according to their purpose. There needs to be further education by health workers to the public to be a preventive measure against the further spread of Covid-19.

Background: Young papaya seeds (Carica papaya L.) contained terpenoids, alkaloids karpain and flavonoids that have been examined has antibacterial activity. Purpose: This study aims to obtain anti-acne cream from extract of young papaya seeds (Carica papaya L.) that possess antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27825. Methods: Extract of young papaya seeds (Carica papaya L.) was obtained by maceration process using ethanol 96% and the antibacterial activity of extract conducted by well diffusion method. then the extract formulated into anti acne cream dosage and then tested to characteristic properties including pH test, dispersive power test, organoleptic test, homogeneity test, viscosity test and irritation test. Result: The results showed anti acne creams of extract young papaya seeds (Carica papaya L.) produced qualified standard and did not cause irritation. Conclusion: Anti acne cream containing extract concentration of 10% can inhibited bacteria of S. aureus ATCC 25923 with inhibition zone of 17,5 mm and P. aeruginosa ATCC 27825 with inhibition zone of 19,3 mm.Keyword: seeds of papaya (Carica papaya L.), anti acne creamLatar Belakang: Biji pepaya muda (Carica papaya L) mengandung terpenoid, alkaloid karpain dan flavonoid, yang telah diteliti memiliki aktivitas antibakteri. Tujuan: Penelitian ini bertujuan untuk memperoleh sediaan krim anti jerawat dari ekstrak biji pepaya muda (Carica papaya L) yang memiliki aktivitas anti bakteri terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aureginosa ATCC 27825. Metode: Ekstrak biji pepaya muda (Carica papaya L) diperoleh melalui proses maserasi menggunakan pelarut etanol 96%, serta uji aktivitas antibakteri ekstrak dilakukan dengan metode sumuran. Ekstrak selanjutnya diformulasikan menjadi sediaan krim anti jerawat dan dilakukan uji karakterisasi meliputi uji pH, uji daya sebar, uji organoleptik, uji homogenitas, uji viskositas dan uji iritasi. Hasil: Hasil pengamatan menunjukkan sediaan krim anti jerawat ekstrak biji pepaya muda (Carica papaya L) yang dihasilkan memenuhi syarat standar nilai pH, daya sebar dan viskositas. Hasil uji iritasi memperlihatkan formula krim tidak menimbulkan iritasi. Simpulan: Sediaan krim anti jerawat dengan konsentrasi ekstrak 10% dapat menghambat bakteri S.aureus ATCC 25923 dengan hambatan sebesar 17,5 mm dan P. aeruginosa ATCC 27825 dengan hambatan sebesar 19,3 mm.Kata Kunci: biji pepaya muda (Carica papaya L), krim anti jerawat

Pare adalah tanaman yang sering dimanfaatkan sebagai obat tradisional dalam menyembuhkan berbagai jenis penyakit, diantaranya diabetes mellitus. Kandungan charantine, polypeptide-p, vicine dan antioksidan memiliki potensi menurunkan kadar glukosa darah. Penelitian ini bertujuan untuk mengetahui aktivitas anti diabetik fraksi ekstrak buah pare dalam menurunkan kadar glukosa darah dan gambaran histopatologi jaringan pankreas tikus yang diinduksi streptozotocin. Penelitian ini menggunakan post test only control group design. Sampel penelitian ini terdiri dari 5 kelompok yaitu kelompok kontrol positif, kelompok kontrol negatif, kelompok fraksi etanol ekstrak buah pare, kelompok fraksi etil asetat ekstrak buah pare, dan kelompok n-heksan ekstrak buah pare. Pengukuran kadar glukosa darah dilakukan pada saat sebelum dilakukannya induksi STZ, setelah induksi STZ, hari ke 4 dan hari ke 7 pemberian fraksi ekstrak buah pare menggunakan glukometer dan spektrofotometer. Pada hari ke 7 dilakukan pembedahan untuk pengambilan sampel darah dan pankreas tikus. Analisis data yang digunakan adalah kruskal-wallis dilanjutkan uji mann-withney. Berdasarkan hasil penelitian, diketahui terdapat perbedaan yang signifikan antara penurunan kadar glukosa darah tikus diabetes mellitus yang diberi fraksi n-heksan ekstrak buah pare dengan kelompok kontrol negatif (p-value

Penyakit hati adalah jenis penyakit yang berbahaya dan masih tergolong tinggi di Indonesia. Salah satu cara untuk mengetahui kerusakan hati dengan mengukur aktivitas enzim SGOT dan SGPT. Salah satu bahan alam yang berpotensi memiliki efek hepatoprotektor adalah tumbuhan Galing (Cayratia trifolia L.Domin) dan telah dilakukan penelitian bahwa tumbuhan Galing mengandung senyawa flavonoid yang berpotensi sebagai antioksidan. Tujuan penelitian adalah untuk mengetahui efek hepatoprotektor dan dosis yang efektif dari ekstrak etanol daun Galing pada tikus putih yang diinduksi paracetamol dosis toksik. Jenis penelitian adalah eksperimental laboratorium, dengan desain Rancangan Acak Lengkap (RAL) dan menggunakan 15 ekor tikus putih yang dibagi dalam 5 kelompok perlakuan dengan tiga kali pengulangan yang diinduksi dengan paracetamol dosis toksik setelah pemberian ekstrak. Berdasarkan hasil penelitian menunjukkan bahwa pemberian ekstrak etanol daun galing dengan dosis 300 mg/kg BB, 400 mg/kg BB, dan 500 mg/kg BB memberikan efek hepatoprotektor dengan mencegah peningkatan kadar SGOT dan SGPT setelah pemberian paracetamol dosis toksik, dan berdasarkan uji LSD dosis 500 mg/kg BB memiliki efek yang paling baik terhadap peningkatan kadar SGOT dan SGPT. Kata kunci : Hepatoprotektor, daun galling, SGOT, SGPT, Rattus norvegicus. ABSTRACT The liver disease is a dangerous disease and still relatively high in Indonesia. One way to determine liver damage by measuring the activity of SGOT and SGPT enzyme. One of the natural substances that potentially have hepatoprotective effects is Galing (Cayratia trifolia L.Domin) and has carried out the result that Galing is potentially containing flavonoids as antioxidants. The purpose of the study is todetermine the effect of hepatoprotective and the effective dose of ethanol extract of Galing leaves rats that induced paracetamol of toxic dose. This type of research is an experimental laboratory, with the design of Completely Randomized Design (CRD) and using 15 rats were divided into 5 groups with three repetitions induced by paracetamol of toxic dose after extract. Based on research data showed that the ethanol extract of galing leaves at a dose of 300, 400, and 500 mg/kg BW provide hepatoprotective effect by preventing an increase in SGOT and SGPT after giving paracetamol of toxic dose, a dose of 500 mg/kg BW have the best effect on levels of SGOT and SGPT. Keywords : Hepatoprotective, Leaf Galing, SGOT, SGPT, Rattus novergicus.

Background: Abelmoschus manihot L.are widely used as a daily dish of dishes and traditional medicine to treat various diseases. It contains alkaloids, triterpenoids, saponins, and flavonoids wich can be toxic agent at variable dose. Purpose: This study aimed determine the effect of ethanol extract of abelmoschus manihot L. on histopatologic image of mice liver tissue. Methods: This is quasi experimental study with post test only control group design. Fourty five mice (mus musculus) as the subject of this research, they divided into 5 groups, group I threated by 50 mg /kgBB extract, group II threated by 200 mg / kgBB extract, group III threated by 800 mg / kgBB extract, group IV threated by 3,200 mg / kgBB extract, and group V as negative control. Treatment was done for 14 days and terminated at next day for histopatology examination use them liver tissue. Result: Histopathological examination of liver cells within normal limits. In the treatment group of 50 mg liver tissue of mice who died during treatment on day 7 and day 10 showed liver tissue necrosis. In the treatment group of 200 mg and 800 mg of liver tissue showed the presence of liver cell necrosis, and cell boundaries appeared unclear. In the treatment group of 3200 mg liver tissue showed necrotic cells, lysi s cells, unclear cell boundaries and necrotic cells more widely.Conclusion: As a conclusion there an histopatologic changes of microscopic examination of mice liver cells in the form of necrosis and, there is not significant difference in ALT and AST between the control group.Keywords : Abelmoschus manihot L. extract, acute toxicity, liver histopathology

Background: Green gedi leaves (Abelmoschus manihot (L.) Medik) is one plant that has efficacy in treating various diseases one of which is antidiabetic. The nature of the active substance green gedi leaves that capable lower blood glucose levels are flavonoids. Purpose: The purpose of this study was to determine the effective concentration of the extract of green gedi leaves (Abelmoschus manihot (L.) Medik) to lower the blood glucose levels on mice Induced by Streptozotocin. Method: This type of research is experimental research that consists of 5 treatments with 3 variations dose and 2 controls. The study was conducted by measuring the blo od glucose levels in mice, blood glucose levels after induction to return normal blood glucose levels after oral administration with a suspension of Na. CMC as a control, and the suspension glibenclamid as a comparison with the extract of green gedi leaves concentration of 30 mg/KgBB, 60 mg/KgBB and 90 mg/KgBB with a time of observation for seven days. Result: The data obtained were analyzed using ANOVA and LSD. Conclusion: From this study that the green gedi leaf extract (Abelmoschus manihot (L.) Medik) at a concentration of 90 mg/kgBB is more effectife to give antidiabetic effect with the average reduction in blood glucose levels reach 68,67 mg/dL to mice.Keywords: antidiabetic, abelmoschus manihot (L.) Medik, green gedi leaves, Streptozotocin

Background: Infectious diseases are one of the major causes of death worldwide, mostly caused by bacteria. The use of less targeted antibiotics leads to resistance cases, thus encouraging the discovery of alternative medicine.In general, gedy leaves are known as animal feed but gedy leaves contain antibacterials such as flavonoids, saponins, tannins and terpenoids. Research Purpose:This study aims to determine the antibacterial activity of ethanol extract of gedy leaves on the growth of E. coli and S. aureus. Research Method: This research uses post test only control design, free variable is ethanol extract of gedi leaves and dependent variable is bacterial growth inhibition zone. Ginger leaf ethanol extract was obtained by maceration method for 3 x 24 hours. The test of antibacterial activity using the well diffusion method. Research result: The results of ethanol extract of gedi leaf with concentration of 2.5 mg/ml, 1.25 mg/ml and 0.625 mg/ml resulted in inhibition zone to E. coli growth successively; they were 11.33 mm, 8 mm and 6.3 mm. Extracts of the same concentration also showed inhibition of S. aureus growth with inhibit zone of 8 mm, 3.6 mm and 2 mm. Conclution:The conclusion of this research is the extract ethanol of gedi leaf has antibacterial activity against E. coli and S. aureus.Keywords: Antibacterial,E. coli and S. aureus , Gedi leaf (Abelmoschus manihot L.), Infection.

Hyperlipidemia is an increase of lipid due to excessive fat consumption, that the intake and alteration of lipid is not balanced. Notika are plants that contain secondary metabolites in the form of flavonoids, tannins, saponins and triterpenoids which are antioxidant compounds that are thought to have the effect of reducing blood lipids. This study aims to determine the effect of ethanol extract of leaves of Notika on decreasing total cholesterol levels in rats induced by egg and propylthiouracil (PTU). This research was experimental in 6 groups, namely 4 groups given ethanol extract of leaves of Notika at a dose of 50 mg/kgBB, 100 mg/kgBB, 200 mg/kgBB, and 300 mg/kgBB, one group was given Na CMC 0.5% as a negative control, and one group was given simvastatin 10 mg as a positive control. The results showed that the ethanol extract of the leaves of Notika significantly reduced the total cholesterol level of rats with a significant value 0.05 and 300 mg/kgBB is an effective dose to reduce total cholesterol level.

Jerawat merupakan gangguan pada kulit yang ditandai dengan adanya peradangan yang disertai penyumbatan pada saluran kelenjar minyak dalam kulit yang disebabkan oleh bakteri Propionibacterium acne . Salah satu tanaman obat yang dapat dimanfaatkan sebagai obat jerawat adalah daun sirih. Daun sirih diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Ekstrak dipurifikasi dari klorofilnya dengan metode ekstraksi cair-cair menggunakan pelarut n -heksan. Penelitian ini bertujuan untuk mengetahui efek antibakteri terhadap ekstrak etanol daun sirih terpurifikasi dan sediaan krim dengan basis vanishing cream terhadap bakteri P. acne dengan metode sumuran. Uji stabilitas fisik sediaan krim meliputi pemeriksaan organoleptis, homogenitas, daya sebar, viskositas, pH, dan tipe krim m/a. Hasil penelitian menunjukkan bahwa ekstrak etanol daun sirih terpurifikasi dengan konsentrasi 0,5% memiliki daya hambat yang lemah, 1% memiliki daya hambat yang sedang, 1,5% dan 2% memiliki daya hambat pertumbuhan P. acne yang kuat. Konsentrasi ekstrak dalam pembuatan krim antijerawat dengan basis vanishing cream sebesar 0,5% memiliki daya hambat yang lemah, 1% dan 1,5% memiliki daya hambat yang sedang, 2% memiliki daya hambat yang kuat sedangkan kontrol positif (asam retinoat) yang digunakan memiliki daya hambat yang kuat terhadap pertumbuhan P . acne . Krim antijerawat dengan basis vanishing cream dengan konsentrasi ekstrak 2% memiliki aktivitas antibakteri yang paling baik terhadap P. acne. Kata kunci: Anti jerawat, daun sirih, krim m/a, Propionibacterium , antibakteri

Latar Belakang: Pada pertengahan 2017 di Kendari telah terjadi kasus penyalahgunaan narkoba yang banyakmemakan korban sebanyak 146 orang, yang di antaranya remaja sebagai salah satu korban terbanyak.Penyalahgunaan obat yang dimaksudkan adalah obat PCC (Paracetamol, Cafein, dan Carisoprodol), atau dalamistilah pemakai biasa disebut pil PCC. Tujuan Penelitian: Penelitian ini bertujuan untuk mengetahui pengaruhpendekatan keperawatan transkultural terhadap pasien pengkonsumsi pil PCC di Kendari. Metode Penelitian:Jenis penelitian yang digunakan pada penelitian ini adalah quasi-experimental dengan pendekatan post test onlycontrol group design, besaran sampel 59 responden. Populasi dalam penelitian ini adalah seluruh pengkonsumsi pilPCC yang terdata oleh BNN Kota Kendari. Metode analisis data menggunakan metode analisis univariat danbivariat dengan interpretasi analisis data r. Hasil Penelitian: Hasil penelitian menunjukkan bahwa ada pengaruhpendekatan transkultural nursing dalam mengkonsumsi pil PCC di Kota Kendari (p-value = 0,005

Penyakit tidak menular (PTM) merupakan penyakit yang tidak ditularkan dan tidak ditransmisikan kepada orang lain dengan bentuk kontak apapun, menyebabkan kematian dan membunuh sekitar 35 juta manusia setiap tahunnya, atau 60% dari seluruh kematian secara global, dengan 80% pervalensi pada negara berkembang. Penyakit tidak menular, khususnya penyakit kardiovaskuler, kanker, penyakit pernapasan kronis, dan diabetes merupakan ancaman utama bagi kesehatan dan perkembangan manusia saat ini. Tujuan pengabdian masyarakat yang terintegrasi kuliah kerja nyata (KKN) Tematik, untuk mendeteksi faktor risiko penyakit tidak menular, pada masyarakat Kecamatan Sampara, Kabupaten Konawe. Metode kegiatan berupa penyuluhan kesehatan, pemeriksaan tekanan darah, kadar kolesterol, gula darah sewaktu dan asam urat. Telah dilakukan skrining dan deteksi dini fator risiko penyakit tidak menular terhadap 67 orang responden, meliputi 14 laki–laki dan 53 perempuan. Pada hasil pemeriksaan tekanan darah, didapatkan jumlah yang mengalami hipertensi 28,35 %, normotensi 67,16, dan hipotensi 4,47 %. Pada pemeriksaan Kolesterol Total, didapatkan kolesterol dalam batas normal 2,38 % dan tinggi 37,31 %. Hasil pemeriksaan gula darah sewaktu (GDS), didapatkan kadar GDS dalam batas normal 85,07 % dan kadar GDS tinggi 14,92 %. Pada pemeriksaan Asam Urat didapatkan dalam batas normal adalah 91,04 % dan tinggi 8,95 %. Kata kunci : faktor risiko; penyakit tidak menular Early Detection of Non-communicable Disease Risk Factors in Andepali Village Community, Sampara District, Konawe Regency ABSTRACT Non-communicable disease (PTM) is a disease that is not transmitted and is not transmitted to others by any form of contact, causing death and killing around 35 million people each year, or 60% of all deaths globally, with 80% prevalence in developing countries. PTM, especially cardiovascular disease, cancer, chronic respiratory diseases, and diabetes are the main threats to human health and development today. The purpose of integrated community service is the Thematic Real Work Study (KKN), to detect risk factors for non-communicable diseases, in the community of Sampara District, Konawe Regency. The method of activity in the form of health education, blood pressure, cholesterol levels, blood sugar and uric acid. Screening and early detection of risk factors for non-communicable diseases have been carried out on 67 respondents, including 14 men and 53 women. On the results of blood pressure examination, it was found that the amount of hypertension was 28.35%, normotension 67.16% and hypotension 4.47%. In the examination of Total Cholesterol, cholesterol was found to be within the normal limit of 2.38% and 37.31% high. Examination results of Blood Sugar (GDS), obtained levels of GDS in the normal range of 85.07% and high GDS levels of 14.92%. In the examination of Gout found in the normal range is 91.04% and high 8.95%. Keywords: gout; blood sugar; cholesterol; non-communicable diseases; blood pressure

Background: Free radicals and antioxidant were widely discussed in world of health because most of the diseases were initiated by presence of oxidation reaction in the body. The process of free radical formation could be inhibited by the presence of antioxidant. Antioxidant was a compound that can protect the biological system in the body. Research Purpose: This research aimed to analyze the potential of fruits and seeds of Morinda citrifolia L. as antioxidant which could counteract free radicals using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Research Method:This research used experimental method with post test only control design. The research was located in Integrated Laboratory of Faculty of Medicine and Pharmacy Laboratory of Faculty of Pharmacy of Halu Oleo University. Samples of this research were fruit and seed ethanol extract of Morinda citrifolia L.which were taken in West Kendari, Kendari City. The independent variables from this research were fruit ethanol extract and seed ethanol extract of cheese fruit and dependent variable was antioxidant potential. Potential and antioxidant activity (IC50) were tested by measuring DPPH (1,1-diphenyl-2-picrylhydrazyl) uptake using a spectrophotometer. Antioxidant activity (IC50) was analyzed using a linear regression equation. Research Result:The result of this research showed that there was a color changing of DPPH from purple to yellow on fruits ethanol extract and seeds ethanol extract of Morinda citrifolia L. and had antioxidant activity about IC50 398,99 ppm and 401,37 ppm, respectively.Conclusion: Conclusion from this research revealed that fruit ethanol extract and seed ethanol extract of Morinda citrifolia L.has weak antioxidant activity.Keywords : Antioxidant activity, DPPH method, IC50, Morinda citrifolia L.

Diabetes mellitus is a chronic disease in which the patient has excess glucose levels in the blood. Pare (Momordica charantia L.) is one of the plants that have efficacy in treating various diseases one of them is antidiabetes. Activity of active substances from the pare may play a role in lowering blood sugar levels. This research is done to know the effect of increasing concentration of pare extract to decrease blood glucose level in mice induced by streptozotocin.This study was experimental laboratory, the study was done by measured the initial blood sugar level in mice, sugar content after induction of steptozotosin, and after administration of 100 mg / kgBW extract test, 250 mg / kgBW and 400 mg / kgBW with observation time for 7 days , as well as gave metformin suspension as a positive control.The results showed that the optimum value of antidiabetic activity of ethanol extract of pare fruit at a concentration of 400 mg / kgBW and had an average decrease of 122 mg / dl.Keywords: Antidiabetes, Momordica charantia L., Streptozotocin

Daun notika merupakan tumbuhan yang berasal dari Papua, yang tumbuh di daerah perbukitan yang telah terjadi erosi.Daun notika secara empiris memiliki manfaat menyembuhkan penyakit malaria dan mempermudah proses persalinan. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder dari daun notika, karakterisasi simplisia daun notika dan toksisitas akut menggunakan metode BSLT terhadap larva udang Artemia salina Leach. Ekstrak daun notika diperoleh dengan menggunakan metode maserasi dengan pelarut etanol 96%. Pengujian skrining fitokimia dilakukan dengan metode perubahan warna yang meliputi uji alkaloid, uji flavonoid, uji terpenoid, uji tannin dan uji saponin. Pengujian karakterisasi simplisia dilakukan dengan menentukan parameter spesifik yang meliputi penetapan sari larut air dan penetapan sari larut etanol sedangkan parameter non spesifik meliputi penetapan kadar air dan penetapan kadar abu. Pengujian toksisitas akut menggunakan metode Brine Shrimp Lethality Test (BSLT) dengan menggunakan larva udang Artemia salina Leach. Hasil pengujian skrining fitokimia daun notika menunjukkan hasil positif pada flavonoid, terpenoid, saponin dan tanin. Hasil pengujian karakterisasi simplisia pada daun notika adalah kadar sari larut etanol adalah 50,04%, kadar sari larut air adalah 32,01%, kadar air 3,99 % dan kadar abu adalah 5,11%. Hasil pengujian toksisitas akut dari ekstrak etanol daun notika secara BSLT menunjukan bahwa daun notika tidak bersifat toksik dengan nilai LC 50 1024,19 µg/mL sehingga dapat dimanfaatkan sebagai obat tradisional. Kata kunci: daun notika , BSLT, toksisitas akut, Artemia salina , obat tradisional

Buas-buas (Premna serratifolia Linn.) belongs to the family Verbenaceae has been used as a traditional medicine. The aims of this research are to determine the compound, extract characteristics and potential toxicity (LC50) of ethanol extract of buas-buas leaves. The used method is Brine Shrimp Lethality Test (BSLT). The test consisted of six concentration treatments namely 1000, 750, 500, 250, 100 ppm and negative control with three replications. In each concentration used 10 Artemia salina leach larvae, and observations were made during 24 hours of larvae mortality. The content of phytochemical compounds from the buas-buas leaves are alkaloids, flavonoids, saponins and tannins. Characterization extract showed 62,21% contents of ethanol soluble extract, 41,5% contents of water soluble extract, 14,1% contents moisture and 4,1% contents ash. LC50 value was determined by probit analysis. The result of LC50 is 133,96 ppm. LC50

Pencegahan kejadian DBD dengan penggunaan temefos yang terbuat dari bahan sintetis sudah mengalami resistensi di beberapa daerah sehingga diperlukan larvasida yang berasal dari bahan alam yang efektif. Buah pare yang mengandung alkaloid, flavonoid dan saponin dapat dijadikan alternatif sebagai antiparasit. Penelitian ini bertujuan untuk menguji ekstrak etanol buah pare dan granul ekstrak etanol buah pare (Momordica charantia L.) sebagai larvasida terhadap larva nyamuk Aedes spp. Penelitian ini menggunakan studi eksperimental laboratorium dengan desain pre and post test. Pengujian larvasida ekstrak etanol buah pare dengan konsentrasi 10, 13, 16, dan 19 mg/100 mL. Granul ekstrak etanol buah pare dibuat dengan dengan massa 100 mg, Temefos 0,02 ppm sebagai kontrol positif, akuades sebagai control negatif, serta granul plasebo (non ekstrak). Hasil penelitian menunjukkan nilai LC50 dan LT50 berdasarkan analisis regresi log probit dengan nilai LC50 pada pengamatan 10,080 menit adalah 48,9 mg/100mL dan LT50 pada konsentrasi 19 mg/100 mL adalah 144,543,9771 menit. Kematian larva pada pengujian ekstrak etanol buah pare mencapai 29% pada konsentrasi 16 mg/100 mL dan mortalitas dari pengujian granul ekstrak etanol buah pare adalah 2%. Ekstrak etanol buah pare efektif pada konsentrasi 16 mg/100 mL dan granul ekstrak buah pare tidak efektif sebagai larvisida terhadap Aedes spp.Kata kunci: Momordica charantia L., Aedes spp., granul

This research was carried out to determine the content of secondary metabolites contained in the ethanol extract of bitter melon (Momordica charantia L.) and antipyretic and antiinflammatory effects on mice. Bitter melon extract obtained by maceration method using ethanol 96%. Bitter melo ethanol extract was made in three variations of dose 50 mg / KgBW, 150 mg / KgBW, and 250 mg / KgBW. The content of secondary metabolites determined phytochemical Skrining by TLC (thin layer chromatography) with specific reagent. Antipyretic effect performed by vaccine DPT (Diphtheria Pertussis Tetanus) as inducers of fever and Parasetamol 500 mg as a positive control and antiinflammatory effect using the method of edema artificial on the soles of the mice foot with carrageenan 2% as an aphrodisiac edema and Diclofenac Sodium 50 mg as a positive control. Subjects used is a white male mice were given intraperitoneally (i.p). Analysis of the data using ANOVA statistical analysis and continued with LSD Post Hoc test. Phytochemical screening results showed that ethanol extract of bitter melon contains alkaloids, tannins, flavonoids, saponins, and triterpenoids. The results indicated that ethanol extract of bitter melon at dose of 150 mg/KgBW has antipyretic effect with total temperature decrease 1,1oC and anti-inflammatory effects with the largest of inflammation inhibition percent by 68.57%. One way ANOVA analysis signify a probability value (p) < 0.05 with 95 % of confidence level that indicate a significant influence of ethanol extract of bitter melon to its antipyretic and antiinflammatory effects on male mice.Keywords : Antipyretic, Anti-inflammatory, Momordica charantia, Bitter melon

The plant andong leaf is a medicinal plant that has not been widely used and contains phenolic, flavonoids,tanins and saponins has many benefits, one of which lower blood glucose levels. This study aim to look at the effects of decrease in blood glucose levels in mice that induced by STZs. The method used was experimental research design is RAL with treatment variations leaf extracts 15%, 30% and 45%, glibenclamid as positive control and Na. CMC negative control. The results were analyzed by ANOVA and continued with Duncan. The results showed that there is a very real difference in the treatment given, andong leaf extracts concentrations is themost effective extracts 15% with the average is 17,67 mg/dL on the hour to three.Key words : Leaf andong, antidiabetic, Mus musculus, streptozotocin.