1. Validation of a paraoxon-based method for measurement of paraoxonase (PON-1) activity and establishment of RIs in horses
- Author
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Francesca Bonelli, Micaela Sgorbini, Saverio Paltrinieri, Irene Nocera, Beatrice Ruggerone, and Alessia Giordano
- Subjects
Male ,0301 basic medicine ,Paraoxonasi-1 ,0403 veterinary science ,Reference Values ,oxidative stress ,Medicine ,lcsh:SF1-1100 ,education.field_of_study ,biology ,Paraoxon ,medicine.diagnostic_test ,04 agricultural and veterinary sciences ,lcsh:QH705-705.5 ,horse ,Warmblood ,lcsh:Economic biology ,acute phase proteins ,Population study ,Female ,Horse serum ,medicine.drug ,medicine.medical_specialty ,lcsh:QH426-470 ,040301 veterinary sciences ,Population ,Physical examination ,03 medical and health sciences ,lcsh:QH540-549.5 ,Internal medicine ,Animals ,Horses ,education ,General Veterinary ,Aryldialkylphosphatase ,business.industry ,Paraoxonase ,medicine.disease ,Surgery ,lcsh:Genetics ,030104 developmental biology ,reference intervals ,inflammation ,Paraoxonasi-1, reference intervals, horse ,biology.protein ,lcsh:Animal culture ,lcsh:Ecology ,Hemoglobinemia ,Acute phase proteins, inflammation, oxidative stress ,business ,Blood Chemical Analysis - Abstract
Paraoxonase-1 (PON-1) is an anti-oxidant compound considered as negative acute phase protein in animals (Rossi et al., 2013) and people (Novak et al., 2010). The paraoxon-based method for measurement of PON-1 in equine serum has not yet been validated. The aim of this study is to validate a paraoxon-based method to measure PON-1 and to establish reference intervals (RIs) in healthy horses and foals. 120 horses (40 geldings, 40 stallions, 40 mares; median age: 11 years; 57 Warmbloods, 46 Trotters) and 55 foals (27 females, 28 males; median age: 47 days; 22 Warmbloods, 31 Trotters) considered healthy after physical examination and biochemistry were examined. Horses were grouped by breed: Thoroughbreds, Trotters, Warmbloods, Draft horses and Ponies. Serum PON-1 was measured with an automated spectrophotometer and an enzymatic method validated in other species (Giordano et al., 2013). After the analytical validation (precision, accuracy, interference studies), RIs were determined using the Reference Value Advisor software, according to ASCVP guidelines (Friedrichs et al., 2012). The possible gender-, age- and breed-related differences were statistically investigated. The paraoxon-based method was precise (CVs, International Journal of Health, Animal Science and Food Safety, V. 4, N. 1s (2017)
- Published
- 2018
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