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216 results on '"Paquet, Marilène"'

1. Recording and manipulation of vagus nerve electrical activity in chronically instrumented unanesthetized near term fetal sheep

Author

Castel, Aude, Burns, Patrick M., Benito, Javier, Liu, Hai L., Kuthiala, Shikha, Durosier, Lucien D., Frank, Yael S., Cao, Mingju, Paquet, Marilène, Fecteau, Gilles, Desrochers, André, and Frasch, Martin G.

Subjects
Quantitative Biology - Quantitative Methods, Quantitative Biology - Tissues and Organs
Abstract

Background: The chronically instrumented pregnant sheep has been used as a model of human fetal development and responses to pathophysiologic stimuli. This is due to the unique amenability of the unanesthetized fetal sheep to the surgical placement and maintenance of catheters and electrodes, allowing repetitive blood sampling, substance injection, recording of bioelectrical activity, application of electric stimulation and in vivo organ imaging. Recently, there has been growing interest in pleiotropic effects of vagus nerve stimulation (VNS) on various organ systems such as innate immunity, metabolism, and appetite control. There is no approach to study this in utero and corresponding physiological understanding is scarce. New Method: Based on our previous presentation of a stable chronically instrumented unanesthetized fetal sheep model, here we describe the surgical instrumentation procedure allowing successful implantation of a cervical uni- or bilateral VNS probe with or without vagotomy. Results: In a cohort of 53 animals, we present the changes in blood gas, metabolic, and inflammatory markers during the postoperative period. We detail the design of a VNS probe which also allows recording from the nerve. We also present an example of vagus electroneurogram (VENG) recorded from the VNS probe and an analytical approach to the data. Comparison with Existing Methods: This method represents the first implementation of VENG/VNS in a large pregnant mammalian organism. Conclusions: This study describes a new surgical procedure allowing to record and manipulate chronically the vagus nerve activity in an animal model of human pregnancy., Comment: Accompanying data repository: https://doi.org/10.6084/m9.figshare.7228307.v2

Published
2020
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3. A second-generation eIF4A RNA helicase inhibitor exploits translational reprogramming as a vulnerability in triple-negative breast cancer

Author

Cencic, Regina, primary, Im, Young K., additional, Naineni, Sai Kiran, additional, Moustafa-Kamal, Mohamed, additional, Jovanovic, Predrag, additional, Sabourin, Valerie, additional, Annis, Matthew G., additional, Robert, Francis, additional, Schmeing, T. Martin, additional, Koromilas, Antonis, additional, Paquet, Marilène, additional, Teodoro, Jose G., additional, Huang, Sidong, additional, Siegel, Peter M., additional, Topisirovic, Ivan, additional, Ursini-Siegel, Josie, additional, and Pelletier, Jerry, additional

Published
2024
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5. RAS Transformation Requires CUX1-Dependent Repair of Oxidative DNA Damage

Author

Ramdzan, Zubaidah M, Vadnais, Charles, Pal, Ranjana, Vandal, Guillaume, Cadieux, Chantal, Leduy, Lam, Davoudi, Sayeh, Hulea, Laura, Yao, Lu, Karnezis, Anthony N, Paquet, Marilène, Dankort, David, and Nepveu, Alain

Subjects
Genetics, Cancer, 2.1 Biological and endogenous factors, Aetiology, Development of treatments and therapeutic interventions, 5.1 Pharmaceuticals, Animals, Cell Line, Tumor, Cell Transformation, Neoplastic, Cells, Cultured, Cellular Senescence, DNA Damage, DNA Repair, Genes, ras, Homeodomain Proteins, Humans, Mammary Neoplasms, Experimental, Mice, Transgenic, Nuclear Proteins, Oxidative Stress, Repressor Proteins, Transcription Factors, Biological Sciences, Agricultural and Veterinary Sciences, Medical and Health Sciences, Developmental Biology
Abstract

The Cut homeobox 1 (CUX1) gene is a target of loss-of-heterozygosity in many cancers, yet elevated CUX1 expression is frequently observed and is associated with shorter disease-free survival. The dual role of CUX1 in cancer is illustrated by the fact that most cell lines with CUX1 LOH display amplification of the remaining allele, suggesting that decreased CUX1 expression facilitates tumor development while increased CUX1 expression is needed in tumorigenic cells. Indeed, CUX1 was found in a genome-wide RNAi screen to identify synthetic lethal interactions with oncogenic RAS. Here we show that CUX1 functions in base excision repair as an ancillary factor for the 8-oxoG-DNA glycosylase, OGG1. Single cell gel electrophoresis (comet assay) reveals that Cux1⁺/⁻ MEFs are haploinsufficient for the repair of oxidative DNA damage, whereas elevated CUX1 levels accelerate DNA repair. In vitro base excision repair assays with purified components demonstrate that CUX1 directly stimulates OGG1's enzymatic activity. Elevated reactive oxygen species (ROS) levels in cells with sustained RAS pathway activation can cause cellular senescence. We show that elevated expression of either CUX1 or OGG1 prevents RAS-induced senescence in primary cells, and that CUX1 knockdown is synthetic lethal with oncogenic RAS in human cancer cells. Elevated CUX1 expression in a transgenic mouse model enables the emergence of mammary tumors with spontaneous activating Kras mutations. We confirmed cooperation between Kras(G12V) and CUX1 in a lung tumor model. Cancer cells can overcome the antiproliferative effects of excessive DNA damage by inactivating a DNA damage response pathway such as ATM or p53 signaling. Our findings reveal an alternate mechanism to allow sustained proliferation in RAS-transformed cells through increased DNA base excision repair capability. The heightened dependency of RAS-transformed cells on base excision repair may provide a therapeutic window that could be exploited with drugs that specifically target this pathway.

Published
2014

7. Supplementary Materials and Methods, Supplementary Tables 1 and 2 and Supplementary Figures 1 through 5 from PTP1B Deficiency Enables the Ability of a High-Fat Diet to Drive the Invasive Character of PTEN-Deficient Prostate Cancers

Author

Labbé, David P., primary, Uetani, Noriko, primary, Vinette, Valérie, primary, Lessard, Laurent, primary, Aubry, Isabelle, primary, Migon, Eva, primary, Sirois, Jacinthe, primary, Haigh, Jody J., primary, Bégin, Louis R., primary, Trotman, Lloyd C., primary, Paquet, Marilène, primary, and Tremblay, Michel L., primary

Published
2023
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8. Supplementary Information from PTP1B Deficiency Enables the Ability of a High-Fat Diet to Drive the Invasive Character of PTEN-Deficient Prostate Cancers

Author

Labbé, David P., primary, Uetani, Noriko, primary, Vinette, Valérie, primary, Lessard, Laurent, primary, Aubry, Isabelle, primary, Migon, Eva, primary, Sirois, Jacinthe, primary, Haigh, Jody J., primary, Bégin, Louis R., primary, Trotman, Lloyd C., primary, Paquet, Marilène, primary, and Tremblay, Michel L., primary

Published
2023
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10. Data from PTP1B Deficiency Enables the Ability of a High-Fat Diet to Drive the Invasive Character of PTEN-Deficient Prostate Cancers

Author

Labbé, David P., primary, Uetani, Noriko, primary, Vinette, Valérie, primary, Lessard, Laurent, primary, Aubry, Isabelle, primary, Migon, Eva, primary, Sirois, Jacinthe, primary, Haigh, Jody J., primary, Bégin, Louis R., primary, Trotman, Lloyd C., primary, Paquet, Marilène, primary, and Tremblay, Michel L., primary

Published
2023
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19. Recording and manipulation of vagus nerve electrical activity in chronically instrumented unanesthetized near term fetal sheep

Author

Castel, Aude, primary, Burns, Patrick M., additional, Benito, Javier, additional, Liu, Hai L., additional, Kuthiala, Shikha, additional, Durosier, Lucien D., additional, Frank, Yael, additional, Cao, Mingju, additional, Paquet, Marilène, additional, Fecteau, Gilles, additional, Desrochers, André, additional, and Frasch, Martin G., additional

Published
2021
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21. Statins downregulate YAP and TAZ and exert anti‐cancer effects in canine mammary tumour cells.

Author

Vigneau, Anne‐Laurence, Rico, Charlène, Boerboom, Derek, and Paquet, Marilène

Subjects
YAP signaling proteins, HIPPO signaling pathway, MAMMARY gland cancer, ANTINEOPLASTIC agents, STATINS (Cardiovascular agents)
Abstract

Canine mammary tumours (CMTs) are the most common neoplasms in intact bitches, and few chemotherapeutic options are available for highly invasive and metastatic tumours. Recent studies have shown the potential involvement of dysregulated Hippo signalling in CMT development and progression. Statins can activate the Hippo pathway by blocking protein geranylgeranylation (GGylation), resulting in decreased expression and activity of the transcriptional co‐activators YAP and TAZ. In this study, we therefore sought to determine if statins could exert anti‐cancer effects in CMT cells. Our results demonstrate that Atorvastatin and Fluvastatin are cytotoxic to two CMT cell lines (CMT9 and CMT47), with ED50 values ranging from 0.95 to 23.5 μM. Both statins acted to increase apoptosis and promote cell cycle arrest. Both statins also decreased YAP and TAZ expression and reduced the mRNA levels of key Hippo transcriptional target genes known to be involved in breast cancer progression and chemoresistance (CYR61, CTGF and RHAMM). Moreover, both statins effectively inhibited cell migration and anchorage independent growth, but did not influence matrix invasion. Taken together, our results demonstrate for the first time that statins act upon the Hippo pathway in CMT cells to counteract several molecular and cellular hallmarks of cancer. These findings suggest that targeting the Hippo pathway with statins represents a novel and promising approach for the treatment canine mammary gland cancers. [ABSTRACT FROM AUTHOR]

Published
2022
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30. Altered prolactin and androgen receptors expression in companion rat benign mammary tumours.

Author

Vergneau‐Grosset, Claire, Cluzel, Caroline, Beauchamp, Guy, Hubbard, Neil E., Paul‐Murphy, Joanne, and Paquet, Marilène

Subjects
ANDROGEN receptors, BENIGN tumors, MAMMARY glands, PROLACTIN, RATTUS norvegicus, PROGESTERONE receptors, ANIMAL welfare
Abstract

Benign mammary tumours are among the most common tumours of companion rats (Rattus norvegicus domestica), as well as a major animal welfare concern and euthanasia. The first objective of this study was to evaluate the expression of oestrogen, progesterone, androgen, and prolactin receptors in neoplastic and normal mammary gland tissues and compare the expression of these receptors between groups. The second objective was to determine if the expression of these receptors in neoplastic mammary gland tissue correlates with overall survival and occurrence of an additional mass after initial mammary mass excision. The third objective was to determine if the expression of oestrogen, progesterone, androgen and prolactin receptors was associated with mammary tumor clinical parameters or with the age of the animals. Thirty‐two benign mammary tumours were collected from companion rats and submitted for immunohistochemistry staining of prolactin receptor, oestrogen receptor alpha (ERa), progesterone and androgen receptors (AR). Allred score were obtained for mammary tumours (n = 32) and surrounding normal mammary tissue (n = 20) when present. Prolactin receptor expression increased significantly with mammary gland tumorigenesis (P <.0001), while AR expression decreased with tumorigenesis (P <.0001). Lower expression of ERa in tumor stroma was associated with shorter survival (P =.02). Hormonal receptor expression was not significantly associated with age, mass diameter, location nor likelihood of additional mass development. Further studies should investigate the effects of prolactin antagonists in a prospective study involving companion rats with benign mammary tumours. [ABSTRACT FROM AUTHOR]

Published
2021
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33. Lats1 and Lats2 are required for the maintenance of multipotency in the Müllerian duct mesenchyme

Author

St-Jean, Guillaume, primary, Tsoi, Mayra, additional, Abedini, Atefeh, additional, Levasseur, Adrien, additional, Rico, Charlène, additional, Morin, Martin, additional, Djordjevic, Bojana, additional, Miinalainen, Ilkka, additional, Kaarteenaho, Riitta, additional, Paquet, Marilène, additional, Gévry, Nicolas, additional, Boyer, Alexandre, additional, Vanderhyden, Barbara, additional, and Boerboom, Derek, additional

Published
2019
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42. Loss of mesenchymal bone morphogenetic protein signaling leads to development of reactive stroma and initiation of the gastric neoplastic cascade

Author

Roy, Sébastien A. B., primary, Allaire, Joannie M., additional, Ouellet, Camille, additional, Maloum-Rami, Faiza, additional, Pomerleau, Véronique, additional, Lemieux, Étienne, additional, Babeu, Jean-Philippe, additional, Rousseau, Jasmin, additional, Paquet, Marilène, additional, Garde-Granger, Perrine, additional, Boudreau, François, additional, and Perreault, Nathalie, additional

Published
2016
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43. PTP1B Deficiency Enables the Ability of a High-Fat Diet to Drive the Invasive Character of PTEN-Deficient Prostate Cancers

Author

Labbé, David P., primary, Uetani, Noriko, additional, Vinette, Valérie, additional, Lessard, Laurent, additional, Aubry, Isabelle, additional, Migon, Eva, additional, Sirois, Jacinthe, additional, Haigh, Jody J., additional, Bégin, Louis R., additional, Trotman, Lloyd C., additional, Paquet, Marilène, additional, and Tremblay, Michel L., additional

Published
2016
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44. Haploinsufficiency of the ESCRT Component HD-PTP Predisposes to Cancer

Author

Manteghi, Sanaz, primary, Gingras, Marie-Claude, additional, Kharitidi, Dmitri, additional, Galarneau, Luc, additional, Marques, Maud, additional, Yan, Ming, additional, Cencic, Regina, additional, Robert, Francis, additional, Paquet, Marilène, additional, Witcher, Michael, additional, Pelletier, Jerry, additional, and Pause, Arnim, additional

Published
2016
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45. Abstract B39: PTP1B deficiency potentiate prostate cancer invasiveness by sensitizing Pten-null tumors to high-fat diet

Author

Labbé, David P., primary, Uetani, Noriko, additional, Vinette, Valérie, additional, Aubry, Isabelle, additional, Migon, Eva, additional, Sirois, Jacinthe, additional, Haigh, Jody J., additional, Lessard, Laurent, additional, Bégin, Louis R., additional, Trotman, Lloyd C., additional, Paquet, Marilène, additional, and Tremblay, Michel L., additional

Published
2016
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47. WNT5a is required for normal ovarian follicle development and antagonizes gonadotropin responsiveness in granulosa cells by suppressing canonical WNT signaling

Author

Abedini, Atefeh, primary, Zamberlam, Gustavo, additional, Lapointe, Evelyne, additional, Tourigny, Catherine, additional, Boyer, Alexandre, additional, Paquet, Marilène, additional, Hayashi, Kanako, additional, Honda, Hiroaki, additional, Kikuchi, Akira, additional, Price, Christopher, additional, and Boerboom, Derek, additional

Published
2015
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49. Targeted ablation of Wnt4and Wnt5ain Müllerian duct mesenchyme impedes endometrial gland development and causes partial Müllerian agenesis†

Author

St-Jean, Guillaume, Boyer, Alexandre, Zamberlam, Gustavo, Godin, Philippe, Paquet, Marilène, and Boerboom, Derek

Abstract

Wnt4and Wnt5ahave well-established roles in the embryonic development of the female reproductive tract, as well as in implantation, decidualization, and ovarian function in adult mice. Although these roles appear to overlap, whether Wnt5aand Wnt4are functionally redundant in these tissues has not been determined. We addressed this by concomitantly inactivating Wnt4and Wnt5ain the Müllerian mesenchyme and in ovarian granulosa cells by crossing mice bearing floxed alleles to the Amhr2crestrain. Whereas fertility was reduced by ∼50% in Wnt4flox/flox; Amhr2cre/+and Wnt5aflox/flox; Amhr2cre/+females, Wnt4flox/flox; Wnt5aflox/flox; Amhr2cre/+mice were either nearly or completely sterile. Loss of fertility was not due to an ovarian defect, as serum ovarian hormone levels, follicle counts, and ovulation rates were comparable to controls. Conversely, the uterus was abnormal in Wnt4flox/flox; Wnt5aflox/flox; Amhr2cre/+mice, with thin myometrial and stromal layers, frequent fibrosis and a >90% reduction in numbers of uterine glands, suggesting redundant or additive roles of Wnt4and Wnt5ain uterine adenogenesis. Loss of fertility in Wnt4flox/flox; Wnt5aflox/flox; Amhr2cre/+mice was attributed to defects in decidualization, implantation, and placental development, the severity of which were proportional to the extent of gland loss. Furthermore, a third of Wnt4flox/flox; Wnt5aflox/flox; Amhr2cre/+females had a partial agenesis of Müllerian duct-derived structures, but with normal oviducts and ovaries. Together, our results suggest that Wnt4and Wnt5aplay redundant roles in the development of the female reproductive tract, and may provide insight into the etiology of certain cases of Müllerian agenesis in women.

Published
2019
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