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Expanding the use of tropical biomass wastes for nitrogen-limited fermentation was investigated, specifically, the production of medium chain length polyhydroxyalkanoates. Comprehensive central composite design was conducted to assess pH, temperature, biomass solid loading, cellulase loading and amylase loading and their impact on the hydrolysis of palm, coconut and cassava wastes. Glucose yields of 33.3, 31.7 and 79.0% wt. with respect to total glucose were found for palm, coconut and cassava, respectively. Importantly, the impact on the total nitrogen derived during enzymatic hydrolysis of these tropical biomass was described for the first time. The level of nitrogen needs to be properly controlled as high nitrogen would result in low carbon to nitrogen ratio leading to low polyhydroxyalkanoates accumulation, but low nitrogen would hinder growth of the biopolymer producer. Maximum hydrolysate nitrogen, were 1.80, 1.55 and 0.871 g/l for palm, coconut and cassava, respectively. Using the surface responses, biomass media designed for high carbon-to-nitrogen were produced and validated using Pseudomonas putida. Low glucose-carbon to nitrogen were found for palm and coconut after scale-up, leading to the majority of their polyhydroxyalkanoates not being biomass-derived. However, cassava-derived biopolymers were successfully accumulated at 9.01 and 7.13% wt. for total medium chain length polyhydroxyalkanoates and 10-carbon polyhydroxyalkanoates, respectively. This study provides an important foundation for the expansion of tropical biomass wastes for biopolymer production and other nitrogen-limited applications in general.

The alternative approach to the production of biodiesel from sludge palm oil (SPO) via two steps of extraction and enzymatic esterification was performed. Free fatty acids (FFAs) from SPO were obtained through saponification reaction and subsequent extraction. The FFAs were further converted to biodiesel (fatty acids methyl esters) via esterification reaction catalysed by immobilised Rhizopus oryzae lipase on alginate-polyvinyl alcohol (PVA) beads. The esterification reaction was investigated by variations of temperature (30–50 °C), molar ratio of methanol to FFAs (1:1 to 5:1), agitation rate (100–300 rpm) and for 4 h. The results were revealed that optimal condition for biodiesel production reached at reaction temperature of 40 °C, methanol to FFAs molar ratio of 3:1 and agitation rate of 200 rpm. Under the optimum condition, the highest biodiesel yield of 91.30% was achieved. Successfully, biodiesel production from SPO gave high conversion rate. In addition, the biocatalyst also showed in high catalytic efficiency and stability up to 15 cycles. The process cost could be reduced cost effectively for biodiesel production when a low cost substrate of SPO as well as the capable biocatalyst were used.

In this work, water hyacinths, bagasse and rice straw were valorized to produce an innovative biopolymer. Serial steps of extraction, bleaching and conversion of cellulose to be carboxymethylcellulose (CMC) as well as the last steps of blending and molding were performed. The CMC was mixed with tapioca starch solution by a ratio of 9:18, and a plastic sizer of glycerol was varied at 2%, 4% and 6% by volume. In addition, bioplastic sheets were further determined in their properties and biodegradation. The results revealed that bioplastics with 6% glycerol showed a high moisture content of 23% and water solubility was increased by about 47.94% over 24 h. The effect of temperature on bioplastic stability was found in the ranges of 146.28–169.25 °C. Furthermore, bioplastic sheets with 2% glycerol could maintain their shape. Moreover, for texture analysis, the highest elastic texture in the range of 33.74–38.68% with 6% glycerol was used. Moreover, bioplastics were then tested for their biodegradation by landfill method. Under natural conditions, they degraded at about 10.75% by weight over 24 h after burying in 10 cm soil depth. After 144 h, bioplastics were completely decomposed. Successfully, the application of water, weed and agricultural wastes as raw materials to produce innovative bioplastic showed maximum benefits for an environmentally friendly product, which could also be a guideline for an alternative to replace synthetic plastics derived from petroleum.

An integrated cleaner biocatalyst process was performed for biodiesel production from crude palm oil (CPO) and refined palm oil (RPO). It was evaluated on process efficiency in terms of high purity of biodiesel as well as by-products without purification, less wastewater, less time consuming, and a simple downstream process. A first saponification step was carried out in both f CPO and RPO, a high purity of glycerol (86.25% and 87.5%) was achieved, respectively, while free fatty acids (FFASs) in soap were obtained after hexane extraction. High yields of FFASs were obtained from both CPO and RPO (98.83% and 90.94%). Subsequently, the FFAs were esterified to biodiesel by a biocatalyst of immobilized lipase. The highest biodiesel yields achieved were of 92.14% and 92.58% (CPO and RPO). Remarkably, biodiesel yields obtained from CPO and RPO achieved satisfactory values and the biocatalyst used could be reused for more than 16–17 cycles.

Palm oil mill effluent (POME), wastewater discharged from the palm oil refinery industry, is classified as an environmental pollutant. In this work, a heterogeneous catalytic process for biojet fuel or green kerosene production was investigated. The enzymatic hydrolysis of POME was firstly performed in order to obtain hydrolysed POME (HPOME) rich in free fatty acid (FFA) content. The variations of the water content (30 to 50), temperature (30 to 60 &deg, C) and agitation speed (150 to 250 rpm) were evaluated. The optimal condition for the POME hydrolysis reaction was obtained at a 50% v/v water content, 40 &deg, C and 200 rpm. The highest FFA yield (Y FA) of 90% was obtained. Subsequently, FFA in HPOME was converted into hydrocarbon fuels via a hydrocracking reaction catalysed by Pd/Al2O3 at 400 &deg, C, 10 bars H2 for 1 h under a high pressure autoclave reactor (HPAR). The refined-biofuel yield (94%) and the biojet selectivity (57.44%) were achieved. In this study, we are the first group to successfully demonstrate the POME waste valorisation towards renewable biojet fuel production based on biochemical and thermochemical routes. The process can be applied for the sustainable management of POME waste. It promises to be a high value-added product parallel to the alleviation of wastewater environmental issues.

A new application of biocomposite hydrogels named gelatin-alginate (GA) and pectin alginate (PA) enables the use of the hydrogels as carriers for lipase entrapment during biodiesel production. Waste frying acid oil (WFAO), a raw material, was converted to biodiesel via an esterification reaction catalysed by two different immobilised biocatalysts: gelatin-alginate lipase (GAL) and pectin-alginate lipase (PAL). The highest immobilisation yield of GAL and PAL beads was achieved at 97.61% and 98.30%, respectively. Both of them gave biodiesel yields in the range of 75&ndash, 78.33%. Furthermore, capability and reusability of biocatalysts were improved such that they could be reused up to 7 cycles. Moreover, the predicted biodiesel properties met the European biodiesel standard (EN14214). Interestingly, entrapped lipase on composite hydrogels can be used as an alternative catalyst choice for replacing the chemical catalyst during the biodiesel production.

Graphical abstract, Typically, sludge palm oil (SPO) which discharged from the palm oil refining process, is a low cost material of potential value, due to its high free fatty acid content. Accordingly, there is potential for upgrading low grade oil into valuable biofuel. In this work, we present a novel method for biodiesel production from SPO. The process consists of two steps (i) free fatty acid (FFA) extraction (ii) enzymatic esterification. Firstly, SPO was saponified by hydroalcoholic solution into soap and glycerol. Secondly, the FFAs obtained were further converted into biodiesel via enzymatic esterification catalyzed by immobilised alginate-PVA lipase beads. • Biodiesel production from sludge palm oil could be completed. • A modified fatty acid extraction was used for SPO fatty acid preparation. • Immobilised alginate-PVA lipase beads were used as biocatalyst for esterification reaction.

In this work, two kinds of catalyst called monometallic Palladium (Pd) and a bimetallic of Pd-Iron (Fe) were synthesised using aluminum oxide (Al2O3) as the supported material via the wet impregnate method. A monometallic catalyst (0.5% Pd/Al2O3) named Pd cat was used as control. For the bimetallic catalyst, ratios of Pd to Fe were varied, and included 0.38% Pd&ndash, 0.12% Fe (PF1), 0.25% Pd&ndash, 0.25% Fe (PF2), and 0.12% Pd&ndash, 0.38% Fe (PF3). The catalysts were characterised to investigate physical properties such as the surface area, pore size, porosity, and pore size distribution including their composition by Brunauer&ndash, Emmett&ndash, Teller (BET) surface area, Scanning Electron Microscopy (SEM), and X-Ray Diffraction (XRD). Subsequently, all catalysts were applied for biofuels production in terms of green diesel/kerosene/gasoline from palm oil via a hydrocracking reaction. The results showed that the loading of Fe to Pd/Al2O3 could improve the active surface area, porosity, and pore diameter. Considering the catalytic efficiency for the hydrocracking reaction, the highest crude biofuel yield (94.00%) was obtained in the presence of PF3 catalyst, while Pd cat provided the highest refined biofuel yield (86.00%). The largest proportion of biofuel production was green diesel (50.00&ndash, 62.02%) followed by green kerosene (31.71&ndash, 43.02%) and green gasoline (6.10&ndash, 8.11%), respectively. It was clearly shown that the Pd-Fe bimetallic and Pd monometallic catalysts showed potential for use as chemical catalysts in hydrocracking reactions for biofuel production.

Palm oil soap stock (POSS) was recycled from bio-waste to bio-fuel. Firstly, POSS was converted through acidification to be acidified POSS (APOSS) coupled with solvent extraction. APOSS with a high content of free fatty acids (FFAs) (90.94%) was obtained. Subsequently, the FFAs in APOSS were converted to biodiesel via esterification, catalyzed by immobilized alginate-polyvinyl alcohol (ALG-PVA) lipase. The key factors affecting the reaction, such as the temperature (30–50 °C), methanol to FFA molar ratio (2:1 to 4:1), and agitation rate (150–250 rpm) were optimized by statistical response surface methodology (RSM). The highest biodiesel yield was achieved with a reaction temperature of 40 °C, methanol/FFA molar ratio of 3:1, and 200 rpm agitation rate, as the optimal conditions. Furthermore, the biocatalyst can be reused up to 16 cycles. Interestingly, the alternative biodiesel production process reported herein promotes zero waste from the palm oil refinery process.

The green microalga, Scenedesmus incrassulatus PPAY1 was cultivated in vertical tubular photobioreactor under batch/fed-batch modes. Biodiesel derived glycerol waste (BDGW) was applied as a cheap main nutrient source. For batch cultivation (BC), maximum biomass concentration was reached at 2.83 gL-1when 20 gL-1 BGW was used. In contrast, in the case of fed-batch cultivation (FBC), the maximum algae and lipid contents were achieved at 5.65 gL-1 and 58.27%, respectively when 30 and 20 gL-1 BDGW were applied. The highest lipid yield of 2.94 gL-1was found at 30 gL-1 BDGW. The analysis of the fatty acid content revealed that palmitic acid-, oleic acid- and linoleic acid- were mainly found in both BC and FBC conditions. As expected, FBC was in a better culture strategy than BC. BDGW could be used directly without any pretreatment in both algal biomass growth and microalgal oil accumulation which simplifies cultivation and reduces operating costs during biodiesel production.

29 (Supplement), 21, KKU Research Journal