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Most of the heavy metals in urban environments derives from road traffic, particularly from tyres and brake wear (non-exhaust emission sources). These pollutants contaminate the soil, where several organisms have a primary ecosystem role (e.g., springtails, ants, earthworms). Springtails (Collembola) are soil-dwelling animals regulating soil fertility, flow of energy through above- and below-ground food webs, and they contribute to soil microbial community dispersion and biodiversity maintenance. In this study we investigated the ecotoxicological effects of oral exposure to particles emitted from brake pads and cast-iron brake discs in the euedaphic collembola species Orthonychiurus folsomi under laboratory conditions. Our results showed that chronic exposure to brake wear particles can have sub-lethal effects both at low and high concentrations and it can cause histological alterations. Here, SEM-EDX was applied to observe the particulate and we found its chemical markers in the gut and faeces of collembola, while histological analysis detected alterations of the digestive and reproductive systems and of the abdominal fat body at high concentrations.

Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species that causes significant agricultural losses, especially to orchard fruits, vegetables, herbaceous and ornamental plants. It is also a nuisance pest that seeks shelter in indoor spaces during the winter months. Harnessing the H. halys virome can result in new environmentally sustainable approaches to contain its populations and its relatated agricultural damages. In this study, RNA-Seq data were used to explore the virome associated to ten field populations collected in the Lombardy region in Northern Italy. We identified six complete viral genomes, three of which were previously unknown, belonging to the orders Reovirales, Articulavirales, Ghabrivirales, Durnavirales, and Picornavirales. The prevalence of the six viruses was evaluated by Real-time reverse transcription-quantitative PCR on eighty individuals. Halyomorpha halys ifla-like virus 2 turned out to be the most geographically widespread virus, as it was found in more than 50% of the analyzed insects and in nine out of the ten sampling locations. Moreover, in some individuals, this iflavirus was found in association with each of the other viruses in various combinations that involved up to four viruses. Further studies on such virus-virus interactions and their relationships with the insect host may open the possibility to exploit these naturally occurring viruses as specific and targeted biocontrol agents of H. halys.

The concept of ecosystem services is widely understood as the services and benefits thatecosystems provide to humans, and they have been categorised into provisioning, regulating, supporting, and cultural services. This article aims to provide an updated overview of the benefits that the honey bee Apis mellifera provides to humans as well as ecosystems. We revised the role of honey bees as pollinators in natural ecosystems to preserve and restore the local biodiversity of wild plants; in agro-ecosystems, this species is widely used to enhance crop yield and quality, meeting the increasing food demand. Beekeeping activity provides humans not only with high-quality food but also with substances used as raw materials and in pharmaceuticals, and in polluted areas, bees convey valuable information on the environmental presence of pollutants and their impact on human and ecosystem health. Finally, the role of the honey bee in symbolic tradition, mysticism, and the cultural values of the bee habitats are also presented. Overall, we suggest that the symbolic value of the honey bee is the most important role played by this insect species, as it may help revitalise and strengthen the intimate and reciprocal relationship between humans and the natural world, avoiding the inaccuracy of considering the ecosystems as mere providers of services to humans.

Apis mellifera is an important provider of ecosystem services, and during flight and foraging behaviour is exposed to environmental pollutants including airborne particulate matter (PM). While exposure to insecticides, antibiotics, and herbicides may compromise bee health through alterations of the gut microbial community, no data are available on the impacts of PM on the bee microbiota. Here we tested the effects of ultrapure Titanium dioxide (TiO2) submicrometric PM (i.e., PM1, less than 1 µm in diameter) on the gut microbiota of adult bees. TiO2 PM1 is widely used as a filler and whitening agent in a range of manufactured objects, and ultrapure TiO2 PM1 is also a common food additive, even if it has been classified by the International Agency for Research on Cancer (IARC) as a possible human carcinogen in Group 2B. Due to its ubiquitous use, honey bees may be severely exposed to TiO2 ingestion through contaminated honey and pollen. Here, we demonstrated that acute and chronic oral administration of ultrapure TiO2 PM1 to adult bees alters the bee microbial community; therefore, airborne PM may represent a further risk factor for the honey bee health, promoting sublethal effects against the gut microbiota.

Throughout history, honey has been used for many different purposes and due to its medicinal properties, has been one of the products marketed by traders. The figure of the bee first appeared in drawings on rock with the history of humanity, then on statues, as a logo, on money or stamps, and also in movies. Beekeeping museums, which present the historical process of beekeep-ing, also reflect an important culture with their ancient hives, documents, beekeeping materials, and historical antiquities. The contribution of bees to the sustainability of natural life is very important and has a history of 100 million years. The importance of bees and their by‐products is increasing day by day, and the demand for the beekeeping industry as alternative income determines the emergence of new products and activities. Based on its health properties, apitherapy is the basis of activities such as api‐air and api‐diet. In natural regions (i.e., mountainous areas, forests) where beekeeping is carried out, people’s tradition, food culture, and healthy lifestyle attract society’s at-tention. In this context, api‐tourist activity appears as a new phenomenon. In this article, the existing literature was scanned to create a resource about these new fields triggered by the beekeeping sec-tor.

The global demand for bee pollen as a dietary supplement for human nutrition is increasing. Pollen, which comprises proteins and lipids from bees’ diets, is rich in essential amino acids, omega fatty acids, and bioactive compounds that can have beneficial effects on human health. However, bee pollen may also contain contaminants due to environmental contamination. To date, data on bee pollen contamination by environmental pollutants refer almost exclusively to pesticides and heavy metals, and very little information is available on the potential contamination of bee pollen by airborne particulate matter (PM), a ubiquitous pollutant that originates from a wide range of anthropogenic sources (e.g., motor vehicles, industrial processes, agricultural operations). In the present study, pollen grains collected by forager bees living in an industrial area of the Po Valley (Northern Italy) were analyzed for contamination by inorganic PM. The morpho-chemical characterization of inorganic particles using SEM/EDX allowed us to identify different emission sources and demonstrate the potential risk of PM entering the food chain and exposing bees to its ingestion.

Understanding the interaction between insects and microclimate can be essential in order to plan informed and efficient treatments against agricultural pests. Microclimatic factors such as humidity and temperature can influence the population dynamics of the invasive agricultural pest Halyomorpha halys, the brown marmorated stink bug. The aim of this work was to evaluate the level of transpiration of H. halys in dry, normal and humid microclimates according to the sex, physiological conditions and developmental stage of individuals. Water loss during diapause and the effect of population density on insects’ transpiration were also assessed, as were the nutritional preferences of adults upon exiting diapause. Our data demonstrate that microclimatic conditions significantly influence the transpiration of this pest species. The effect of sex and feeding status on insects’ water loss is marked, while population density does not influence water loss in diapausing individuals. The first nutritional need of the overwintering generations is represented by hydration, likely due to the water loss during diapause.

Particulate matter (PM) is a complex mixture of airborne chemical compounds commonly classified by their aerodynamic diameter. Although PM toxicity strongly depends on the morphology, chemical composition, and dimensions of particles, exposure limits set by environmental organisations only refer to the mean mass concentration of PM sampled daily or annually by monitoring stations. In this study, we used honey bees as sensors of airborne PM10 and PM2.5 in a highly polluted area of the Po Valley, northern Italy. Honey bees are an efficient sampler of airborne PM because, during flight and foraging activities, their pubescence promotes the accumulation of electrical charge on the body surface owing to air resistance, thus enhancing airborne PM attraction. Particles attached to the body of bees are readily accessible for physico-chemical characterisation using a scanning electron microscope coupled with X-ray spectroscopy (SEM/EDX). Our results demonstrate that residents in the study area are intermittently but chronically exposed to a well-defined spectrum of metal-bearing particles and mineral phases known to induce specific health outcomes. The morphology, size, and chemical composition of PM10 and PM2.5 detected on bees in the monitoring area were indicative of traffic, agricultural operations, and high-temperature combustion processes. The contribution of the A1 Milano-Bologna highway, local wheat and alfalfa cultivation, and the Parma incineration plant were clearly distinguishable. Our data also demonstrated that PM exposure levels may vary sharply throughout the year based on recurrent local activities.

The phytophagous brown marmorated stink bug Halyomorpha halys (Stål) is known to exhibit cannibalistic behaviour towards eggs. Here, we provide evidence of cannibalism among overwintering H. halys adults. Since diapausing individuals have high physiological demands for surviving long periods under stressful conditions, including the risk of depletion of metabolic reserves and desiccation, we assumed that nutritional and water requirements can be met by intraspecific predation. The role of aggregative behaviour in promoting cannibalism is also discussed. Given its evolutionary advantage, this trait should be maintained over generations and may be more widespread than previously considered in species that display aggregative behaviour during adverse seasons.