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Made available in DSpace on 2021-06-25T11:03:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01 BACKGROUND: Some antioxidants have been used in semen extenders to reduce adverse effects caused by excessive reactive oxygen species (ROS) production. The study was carried out to assess the effect of quercetin (QC) antioxidant therapy on goat semen submitted to cryopreservation. OBJECTIVE: To evaluate the effect of quercetin incorporation in different phases of the cryopreservation process of goat spermatozoa. MATERIALS AND METHODS: Five ejaculates from each of four goats (n= 20) were collected and split into four groups: Control (G1), without QC; G2, 15 μM of QC added to semen before centrifugation; G3, 15 μM QC added to semen after centrifugation; G4, 15 μM QC added to semen before centrifugation and 15 μM of QC added to semen after centrifugation (total of 30 μM of QC); and cryopreserved. All semen samples were evaluated after thawing for sperm kinetics, plasma membrane integrity, and ROS levels. RESULTS: Although lower concentrations of ROS were associated with groups that received antioxidant supplementation (P=0.0213), linear and dose dependent (P

Made available in DSpace on 2019-10-04T15:40:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-05-01. Added 1 bitstream(s) on 2021-07-15T14:35:12Z : No. of bitstreams: 1 S0102-09352019000300752.pdf: 372642 bytes, checksum: 8143e1b4c8e8aa68be8616d26b848594 (MD5) The aim of this study was to compare the effect of the addition of seminal plasma from high and low fertility stallions on sperm viability offrozen-thawed sperm cells from ejaculate and from epididymal tail of subfertile stallions. Six stallions with a history of subfertility were used. After collection, ejaculate spermatozoa were divided into three aliquots: Botu-Semen (R) (EJ-CT); High-quality seminal plasma (EJ-PS1); Low-quality seminal plasma (EJ-PS2). The same was done with sperm cells from epididymis tail after orchiectomy (EP-CT; EP-PS1; EP-PS2). Evaluations of sperm kinetics were assessed by CASA and membrane and acrosome integrity, DNA fragmentation, sperm capacitation and sperm peroxidation were assessed by flow cytometry. After thawing, no differences were observed between ejaculated sperm (EJ) and epididymal sperm (EP) in any CASA evaluations. However, higher (P< 0.05) percentage of cells with intact plasma and acrossomal membranes was observed in EP groups (EP-CT:31.7 +/- 7.5b; EP-PS1:35.2 +/- 7.0b; EP-P52:33.9 +/- 7.2b) compared to EJ groups (EJ-CT: 15.1 +/- 4.9a, EJ-PS1: 11.7 +/- 4.5a, EJ-P52: 13.1 +/- 5,2a). In addition, differences in DNA fragmentation index were observed (EJ-CT:2.6 +/- 0.6a; EJ-PS1:2.4 +/- 0.8a; EJ-PS2:3.0 +/- 0.8a; CT: 1.4 +/- 0.4b; EPPS]: 1.2 +/- 0.3b; EP-1'52: 1.3 +/- 0.2b). It was concluded that the addition of 20% seminal plasma from fertile or subfertile animals prior to the freezing of epididymal spermatozoa from subfertile animals does not interfere in sperm quality. Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil Univ Estadual Paulista, Botucatu, SP, Brazil Univ Fed Vicosa, Vicosa, MG, Brazil Univ Estadual Paulista, Botucatu, SP, Brazil

Made available in DSpace on 2018-12-11T17:03:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Boar spermatozoa arising from the sperm-rich ejaculate fraction are reported to have a more stable plasma membrane and are more resistant to cold shock and premature acrosome reaction than spermatozoa from the whole ejaculate. Furthermore, seminal plasma (SP) can increase the cryotolerance of boar spermatozoa, and in other domestic species, it has the ability to reverse cryopreservation damage. This study aimed to evaluate the effects of boar SP arising from the whole sperm-rich ejaculate fraction (SP-SRF) on the integrity, stability, and peroxidation of sperm membranes after thawing. Each ejaculate (n = 24) was divided among 4 treatments: control (CT), centrifuged and suspended in autologous SP-SRF (CS), centrifuged with withdrawn SP-SRF (CW), and post-thawed SP arising from the whole sperm-rich fraction addition to CW (CWSP). After thawing, all treatments were incubated for 5, 60, and 120 min and were analyzed for membrane integrity, fluidity, and peroxidation by flow cytometer. The absence of SPSRF increased the lipid disorder (P < 0.05) but had no effect on lipid peroxidation (P > 0.05) or membrane integrity (P > 0.05). However, the increase in lipid disorder by withdrawal of SP-SRF was reversed by SP-SRF addition (P < 0.05) to the post-thawing medium, whereas plasma and acrosomal membrane integrity (P > 0.05) and lipid peroxidation (P > 0.05) were unchanged. In conclusion, despite the centrifugation effects, the addition of SP arising from the whole sperm-rich fraction to post-thawed boar semen decreased sperm lipid disorder without an influence of the sperm membrane integrity and peroxidation. Laboratory of Andrology and Technology of Swine Embryos Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of São Paulo Laboratory of Swine Research Department of Animal Nutrition and Production School of Veterinary Medicine and Animal Science University of São Paulo Department of Veterinary Medicine School of Animal Sciences and Food Engineering University of São Paulo Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP) Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP) FAPESP: 2011/23484-8 FAPESP: 2013/08070-8

Made available in DSpace on 2022-04-28T20:07:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-01-01 Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP

Made available in DSpace on 2022-04-29T08:47:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-01-01 Department of Animal Reproduction Veterinary School UNESP SP Department of Animal Reproduction Veterinary School UNESP SP

Made available in DSpace on 2022-04-28T20:07:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-01-01 UNESP-FMVZ Department of Animal Reproduction and Veterinary Radiology UNESP-FMVZ Department of Animal Reproduction and Veterinary Radiology