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Simple Summary: The global distribution of the genus Corbicula is driven by multiple hermaphroditic lineages. In Argentina, there remains a lack of comprehensive understanding of the current distribution, identity, and genetic relatedness of invasive Corbicula lineages. Reviewing 15 populations, we discriminate extant lineages based on both morphology and genetics in Argentina. We identified two lineages: AR morphotype (FW5 haplotype) and CS morphotype (FW17 haplotype). These lineages exhibited virtually segregated distributions, however, intermediate morphotypes found in northeastern Argentina suggest the presence of hybrids. Our study clarifies Corbicula identity and distribution, offering insights into invasion patterns in a wide geographic region. The broad global distribution of freshwater clams belonging to the genus Corbicula is driven by multiple hermaphroditic lineages. These lineages, characterized by shared morphological traits and phenotypic plasticity, pose challenges to morphological identification. Genetic markers, such as the mitochondrial COI gene, play a crucial role in delineating these lineages and their ranges. Morphotypes represent observed phenotypic variations, while lineages are defined based on genetic markers. Here, we comprehensively review Corbicula's distribution in Argentina, discriminate extant lineages based on both morphological and genetic (COI) data, and describe variations in internal and external morphologies using 15 Argentine populations. Genetic analyses identified two mitochondrial lineages: the AR morphotype (FW5 haplotype) and CS morphotype (FW17 haplotype). Strikingly, despite having similar vectors, origins, and invasive stages, Corbicula lineages exhibit virtually segregated distributions. However, mitochondrial haplotypes are found in sympatry mainly in northeastern Argentina where individuals with intermediate morphotypes exist, suggesting the presence of hybrids due to maternal genome retention. These findings contribute to the clarification of the identity and distribution of Corbicula lineages in Argentina, where the genus has been found for over half a century. Similar studies are needed in other areas to better understand the invasion patterns of this successful and adaptable group. [ABSTRACT FROM AUTHOR]

The spread of nonindigenous species by shipping is a large and growing global problem that harms coastal ecosystems and economies and may blur coastal biogeographical patterns. This study coupled eukaryotic environmental DNA (eDNA) metabarcoding with dissimilarity regression to test the hypothesis that ship‐borne species spread homogenizes port communities. We first collected and metabarcoded water samples from ports in Europe, Asia, Australia and the Americas. We then calculated community dissimilarities between port pairs and tested for effects of environmental dissimilarity, biogeographical region and four alternative measures of ship‐borne species transport risk. We predicted that higher shipping between ports would decrease community dissimilarity, that the effect of shipping would be small compared to that of environment dissimilarity and shared biogeography, and that more complex shipping risk metrics (which account for ballast water and stepping‐stone spread) would perform better. Consistent with our hypotheses, community dissimilarities increased significantly with environmental dissimilarity and, to a lesser extent, decreased with ship‐borne species transport risks, particularly if the ports had similar environments and stepping‐stone risks were considered. Unexpectedly, we found no clear effect of shared biogeography, and that risk metrics incorporating estimates of ballast discharge did not offer more explanatory power than simpler traffic‐based risks. Overall, we found that shipping homogenizes eukaryotic communities between ports in predictable ways, which could inform improvements in invasive species policy and management. We demonstrated the usefulness of eDNA metabarcoding and dissimilarity regression for disentangling the drivers of large‐scale biodiversity patterns. We conclude by outlining logistical considerations and recommendations for future studies using this approach. [ABSTRACT FROM AUTHOR]

Invasive freshwater species, such as the exotic mollusc Potamopyrgus antipodarum (New Zealand mudsnail), can frequently survive under harsh conditions, including brackish and hypoxic environments. We experimentally assessed the effects of osmotic (0, 10, 20, 25 and 30 psu) and thermal (20 °C) shock on mortality, activity and physiology of P. antipodarum collected at Capitol Lake, Olympia, Washington, USA, during winter and spring seasons when environmental temperature was 5 and 10 °C respectively. We measured standard metabolic rate and enzymatic activities (malate dehydrogenase, lactate dehydrogenase, alanopine dehydrogenase) in snails after a 10-day acclimation period at high salinity. Significantly higher mortalities were observed at higher salinities; the strongest effects occurred on snails collected at the end of winter, and exposed to 30 psu and 20 °C (100% mortality in 3 days). When snails were collected during the spring, 100% mortality was observed after 40 days at 30 psu and 20 °C. Standard metabolic rates were significantly lower when snails were exposed to salinities of 25 and 30 psu, even after 10 days of acclimation. Enzymatic activities showed small but significant declines after 10 days at 30 psu reflecting the declines observed in overall metabolism. The physiological tolerances to temperature and salinity displayed by this population of P. antipodarum make its eradication from Capital Lake difficult to achieve. [ABSTRACT FROM AUTHOR]

Larval fish development depends largely on their ability to capture and ingest food items, and on food availability. In this context, invasive species, eutrophication and river impoundments have complex impacts on fish larvae. Using samples collected in 2005-2009 in the Salto Grande reservoir (Argentina-Uruguay), periodically affected by cyanobacterial blooms, we studied the impact of the larvae of the exotic bivalve Limnoperna fortunei (Dunker, 1857) (Bivalvia) on larval fish diets. Compared with other nearby waterbodies, the abundance of fish larvae was scarcer in the reservoir, especially during algal bloom periods. Only 20% of the larval fish with gut contents fed on L. fortunei veligers. Seven fish taxa (of a total of 12) consumed veligers of L. fortunei, but only two showed a preference for this prey. Taxonomic changes in the larval fish assemblages due to the river's impoundment, and temporal uncoupling between veliger densities (affected by the toxigenic effects of Microcystis spp.) and ichthyoplankton could account for the comparatively low trophic importance of the invasive bivalve's veligers. These results reflect the complexity of interactions brought about when the same invasive species invades different environments, underscoring that the impacts involved depend as much on the invader, as on the regional and ecological settings of the area invaded. [ABSTRACT FROM AUTHOR]