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This work describes the dosimetry of a two waveguide cell system designed to expose newborn mice to electromagnetic fields associated with wireless fidelity signals in the frequency band of 2.45 GHz. The dosimetric characterisation of the exposure system was performed both numerically and experimentally. Specific measures were adopted with regard to the increase in both weight and size of the biological target during the exposure period. The specific absorption rate (SAR, W kg(-1)) for 1 W of input power vs. weight curve was assessed. The curve evidenced an SAR pattern varying from1 W kg(-1) to6 W kg(-1) during the first 5 weeks of the life of mice, with a peak resonance phenomenon at a weight around 5 g. This curve was used to set the appropriate level of input power during experimental sessions to expose the growing mice to a defined and constant dose.

The aim of this study was to evaluate the cochlear functionality of Sprague-Dawley rats exposed to electromagnetic fields at 900 MHz and to gentamicin by distortion product otoacoustic emissions, which are a well-known indicator of the status of the cochlea's outer hair cells. A population of 32 rats was divided into four groups: group 1 was treated with daily intramuscular injections of 150 mg/kg body weight gentamicin for 15 days; group 2 was treated with daily intramuscular injections of 150 mg/kg body weight gentamicin for 15 days and exposed to electromagnetic fields; group 3 was exposed to electromagnetic fields; group 4 was sham-exposed. Rats were exposed 2 h/day, 5 days/week for 4 weeks at a local SAR of 4 W/kg in the ear (continuous wave at 900 MHz). Distortion product otoacoustic emissions tests were carried out before, during and after the combined exposure. The analysis of the data showed no subchronic exposure to electromagnetic fields on the inner auditory system of rats in either normal ears or ears exposed to a well-recognized pathological agent.

The aim of this study was to evaluate whether daily whole-body exposure to 900 MHz GSM-modulated radiation could affect spleen lymphocytes. C57BL/6 mice were exposed 2 h/day for 1, 2 or 4 weeks in a TEM cell to an SAR of 1 or 2 W/kg. Untreated and sham-exposed groups were also examined. At the end of the exposure, mice were killed humanely and spleen cells were collected. The number of spleen cells, the percentages of B and T cells, and the distribution of T-cell subpopulations (CD4 and CD8) were not altered by the exposure. T and B cells were also stimulated ex vivo using specific monoclonal antibodies or LPS to induce cell proliferation, cytokine production and expression of activation markers. The results did not show relevant differences in either T or B lymphocytes from mice exposed to an SAR of 1 or 2 W/kg and sham-exposed mice with few exceptions. After 1 week of exposure to 1 or 2 W/kg, an increase in IFN-gamma (Ifng) production was observed that was not evident when the exposure was prolonged to 2 or 4 weeks. This suggests that the immune system might have adapted to RF radiation as it does with other stressing agents. All together, our in vivo data indicate that the T- and B-cell compartments were not substantially affected by exposure to RF radiation and that a clinically relevant effect of RF radiation on the immune system is unlikely to occur.

UMTS communication devices are becoming common in everyday use. This could raise public concern about their possible detrimental effects on human health. The aim of this study, in the framework of the EMF nEAR Project, was to evaluate possible influence of UMTS electromagnetic fields (EMF) exposure on cochlear outer hair cells' (OHCs) functionality in laboratory animals. Forty-eight male Sprague–Dawley rats were locally exposed (right ear) or sham-exposed to a controlled UMTS EMF, frequency of 1946 MHz, at SAR level of 10 W/kg, 2 h a day, 5 days a week, for 4 weeks. A group of 12 rats treated with kanamycin (KM) was also included as positive control. Rats were tested by recording Distortion Product Otaoacoustic Emissions (DPOAEs), a non-invasive test capable of assessing the status of the OHCs in the inner ear. DPOAEs were performed before, during (one or three times a week) and after (1-week) exposure to the EMF. The analysis of the data shows that no statistically significant differences were found between the audiological signals recorded from the different experimental groups. The ototoxic effect of KM has been confirmed. Bioelectromagnetics 30:385–392, 2009. © 2009 Wiley-Liss, Inc.

Distortion product otoacoustic emissions (DPOAE) can be used to study cochlear function in an objective and non-invasive manner. One practical and essential aspect of any investigating measure is the consistency of its results upon repeated testing of the same individual/animal (i.e., its test/retest repeatability). The goal of the present work is to propose two indices to quantitatively assess the repeatability of DPOAE in laboratory animals. The methodology is here illustrated using two data sets which consist of DPOAE subsequently collected from Sprague-Dawley rats. The results of these experiments showed that the proposed indices are capable of estimating both the repeatability of the true emission level and the inconsistencies associated with measurement error. These indices could be a significantly useful tool to identify real and even small changes in the cochlear function exerted by potential ototoxic agents.

In recent years, the widespread use of mobile phones has been accompanied by public debate about possible adverse consequences on human health. The auditory system is a major target of exposure to electromagnetic fields (EMF) emitted by cellular telephones; the aim of this study was the evaluation of possible effects of cellular phone-like emissions on the functionality of rat's cochlea. Distortion Products OtoAcoustic Emission (DPOAE) amplitude was selected as cochlea's outer hair cells (OHC) status indicator. A number of protocols, including different frequencies (the lower ones in rat's cochlea sensitivity spectrum), intensities and periods of exposure, were used; tests were carried out before, during and after the period of treatment. No significant variation due to exposure to microwaves has been evidenced.

The aim of this study was to investigate the interaction between a 50 Hz, 2 mT magnetic field (MF) exposure and cell growth of mammary murine adenocarcinoma, injected into experimental mice. Six different experimental protocols were performed over 2 years; several different protocols of timing of exposure were tested. X-ray radiation was adopted as the positive control. Tumor incidence and the tumor development time were calculated. No effect was observed in any experiment, and there was no statistically significant difference related to time courses among the protocols used. Neither the time of tumor cell injection nor the time of exposure produced differences between unexposed, sham, and exposed mice. When X-ray radiation was applied, the cytotoxic effect of ionizing radiation was clear, but was not increased or modified by MF exposure. Finally, the study revealed how the host–tumor system has shown a distinctive variability, unmodified by MF exposure. Bioelectromagnetics 21:608–614, 2000. © 2000 Wiley-Liss, Inc.

Over last decades, the remarkable growth of electromagnetic fields level in the environment, related to industrial and technological development, has been increased the interest on possible interactions between extremely low frequencies (ELF) magnetic fields and cells of several biological systems. The aim of this study was to investigate the effects of magnetic field exposure in an in vivo experimental model; the influence of ELF (in particular 50 Hz) on tumor development has been investigated, testing the effect on malignant cells proliferation and tumoral growth rate.

In the last few years, a great interest has accompained the studies on the effects of extremely low frequencies (ELF) fields on cells of different biological systems. In the present paper, the effects of the magnetic field (MF) exposure on cells of the immune system were investigated in an in vivo experimental model. Results have indicated an effect of the exposure on both cellularities and functions of cells from central and peripheral lymphoid tissues, as these parameters were reduced in mice exposed to an ELF field as compared to unexposed animals.

The purpose of this paper is to present the experimental device and the work in progress performed in search for objective organic correlation of damage to hearing, examining possible acoustic otofunctional effects on the cochlear epithelium of the rat due to exposure to microwaves (900 MHz). Two experiments using male Sprague-Dawley rats were carried out with a far-field exposure in a cubic chamber. No statistically significant evidence was obtained at both specific absorption rate (SAR) values. The exposure system and the diagnostic apparatus are extremely useful to investigate a potential effect on the auditory system: however, with the parameters applied in these experiments, no evidence was observed.