Search

In our previous study, quorum quenching (QQ) bacteria can effectively enhance methane production in an anaerobic membrane bioreactor (AnMBR) while mitigating membrane biofouling. However, the mechanism of such enhancement is unclear. In this study, we analyzed the potential effects from separated hydrolysis, acidogenesis, acetogenesis and methanogenesis steps. The cumulative methane production improved by 26.13%, 22.54%, 48.70% and 44.93% at QQ bacteria dosage of 0.5, 1, 5 and 10 mg strain/g beads, respectively. It was found that the presence of QQ bacteria enhanced acidogenesis step resulting in higher volatile fatty acids (VFA) production, while it had no obvious influence on hydrolysis, acetogenesis and methanogenesis steps. The substrate (glucose) conversion efficiency in acidogenesis step was also accelerated (1.45 folds vs control within first eight hours). The abundance of hydrolytic fermentation gram-positive bacteria and several acidogenic bacteria, such as Hungateiclostridiaceae, was promoted in QQ amended culture, which enhanced VFA production and accumulation. Although the abundance of acetoclastic methanogen Methanosaeta reduced by 54.2% on the 1st day of QQ beads addition, the overall performance of methane production was not affected. This study revealed that QQ had a greater impact on the acidogenesis step in the anaerobic digestion process, though the microbial community in acetogenesis and methanogenesis steps was altered. This work can provide a theoretical basis for using QQ technology to slow down the rate of membrane biofouling in anaerobic membrane bioreactors while increasing methane production and maximizing economic benefits. Nanyang Technological University The authors acknowledge the financial support of Advanced Environmental Biotechnology centre, Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University, Singapore.

In this study, the antitoxicity performance of the traditional anaerobic baffled reactor (ABR) and the newly constructed membraneless anaerobic baffled reactor coupled with microbial fuel cell (ABR-MFC) was compared for the treatment of simulated printing and dyeing wastewater under the same hydraulic residence time. The sludge performances of ABR-MFC and ABR were evaluated on the dye removal rate, extracellular polymer (EPS) content, sludge particle size, methane yield, and the surface morphology of granular sludge. It was found that the maximum power density of the ABR-MFC reactor reached 1226.43 mW/m3, indicating that the coupled system has a good power generation capacity. The concentration of the EPS in the ABR-MFC reactor was about 3 times that in the ABR, which could be the result of the larger average particle size of sludge in the ABR-MFC reactor than in the ABR. The dye removal rate of the ABR-MFC reactor (91.71%) was higher than that of the ABR (1.49%). The methane production and microbial species in the ABR-MFC system were higher than those in the ABR. Overall, the MFC embedded in the ABR can effectively increase the resistance of the reactor, promote the formation of granular sludge, and improve the performance of the reactor for wastewater treatment.

The effects of rice husk flour (RHF), rice husk biochar (RHB), and rice husk-sludge cake biochar (RH-SCB, expresses sludge cake biochar deriving from a sludge that has been previously conditioned with rice husk) used as physical conditioners on sludge dewaterability were compared. The effects of characteristics of physical conditioners on sludge compressibility and zeta potential were analyzed. The optimal rice husk-based powder was RH-SCB, which presented the highest net sludge solid yield (YN, expresses the dry mass flow by filtration) at 20.39 kg/(m2 h) for 70% dry sludge (DS). Characterization analysis indicates that the hardness and surface Fe content of powders which could influence the compressibility coefficient of sludge cake and sludge zeta potential were the major factors influencing sludge dewaterability. The comparison of feasibility and economic analysis showed that adding RH-SCB improves the quality of the sludge filtrate and reduces the pollution potential of conditioned sludge (the ratio of secondary and primary (RSP) of Cu, Zn, Cd reduces from 43.05, 144.00, 7.25 to 7.89, 14.63, 4.27, respectively), and the costs of using RH-SCB were the lowest (at 88.4% lower than that of the raw sludge). Therefore, it is feasible to use RH-SCB to improve sludge dewaterability.

The storage of large amount of power production waste occupies huge land resource; moreover, the stored or discarded waste may pollute the water environment through changing the water pH, releasing the trace and toxic elements even radioactive elements, and so on by leachate. Therefore, the recycling and disposal of power production waste are important and necessary. This paper reviews the research literatures published in 2019 on power generation waste from coal-fired and nuclear power plants, mainly including the recycling of fly ash and flue gas desulfurization gypsum in construction industry and environmental application, the recovery and immobilization of different metals from coal combustion products and selective catalytic reduction catalysts, and the treatment and disposal of radioactive elements from nuclear power plants. Practioner points Coal-fired power plant waste can be applied for material preparation and wastewater purification. Valued and toxic metals are normally recovered or removed from spent selective catalytic reduction catalyst. Recovery and removal of radioactive elements is essential for nuclear power plant wastes disposal.

The pollution of microplastics (MPs) in soil has become a global environmental problem. Due to high sorption capacity and persistence in environment, the MPs exhibit combined effects with organic pollutants in soil, thereby posing a potential risk to soil ecology and human health. However, limited reviews are available on this subject. Therefore, in response to this issue, this review provides an in-depth account of interaction of MPs with organic contaminants in soil and the combined risks to soil environment. The sorption of organic contaminants onto MPs is mainly through hydrophobic and π-π interactions, hydrogen bonding, pore filling and electrostatic and van der Waals forces. The intrinsic characteristics of MPs, organic contaminants and soil are the key factors influencing the sorption of organic pollutants onto MPs. Importantly, the presence of MPs changes the sorption, degradation and transport behaviors of organic contaminants in soil, and affects the toxic effects of organic contaminants on soil organisms including animals, plants and soil microorganisms through synergistic or antagonistic effects. Source control, policy implementation and plastic removal are the main preventive and control measures to reduce soil MPs pollution. Finally, priorities for future research are proposed, such as field investigations of co-pollution, contribution of plastisphere to organic contaminant degradation, and mechanisms of MPs effects on organic contaminant toxicity.

Food waste was used to produce ethanol by yeast fermentation and volatile fatty acids (VFAs) by hydrolytic acidogenesis for chain elongation. Effectiveness of mole ratio of ethanol in yeast fermentation effluent (YFE) to VFAs in hydrolytic acidification effluent (HAE) on chain elongation was examined. The ideal YFE to HAE ratio for chain elongation was 2:1, the highest n-caproate production was 169.76 mg COD/g vS and the food waste utilization was 65.43 %. Electron transfer and carbon distribution did not completely correspond to n-caproate production, suggesting timely product extraction. The abundance of Romboutsia and Clostridium_sensu_stricto_12 increased as chain elongation progressed, which was critical for the chain elongation to n-caproate. The food waste shunting ratio of yeast fermentation to hydrolytic acidogenesis was 6:5, and 572.6 CNY can be created through chain elongation from shunting fermentation of 1 t food waste. This study proposed a new approach for efficient producing n-caproate from food waste.

The quality of groundwater along rivers is greatly affected by long-term infiltration from surface water, especially reclaimed water-receiving rivers. To predict the degree of influence of contaminated river water on groundwater quality, the spatiotemporal distribution and migration evolution prediction of benzo[a]pyrene (B(a)P) was monitored and simulated by Hydrus-coupled Groundwater Modeling Systems (GMS) model in terms of reclaimed water-receiving Liangshui River. The prediction results indicated the goodness-of-fit of this coupled model, according to the model efficiency (E: 0.78-0.93), the mean absolute error (MAE: 0.01-0.32 m) and the root-mean-square error (RMSE: 0.06-0.35 m). The vertical infiltration rate of B(a)P in the vadose zone was 0.102 m

Atrazine has become one of the most popular applied triazine herbicides in the world due to its high herbicidal efficiency and low price. With its large-dosage and long-term use on a global scale, atrazine can cause widespread and persistent contamination of soil and sediment. This review systematically evaluates the toxicological effects, environmental risks, environmental behaviors (adsorption, transport and transformation, and bioaccumulation) of atrazine, and the remediation technologies of atrazine-contaminated soil and sediment. For the adsorption behavior of atrazine on soil and sediment, the organic matter content plays an extremely important role in the adsorption process. Various models and equations such as the multi-media fugacity model and solute transport model are used to analyze the migration and transformation process of atrazine in soil and sediment. It is worth noting that certain transformation products of atrazine in the environment even have stronger toxicity and mobility than its parent. Among various remediation technologies, the combination of microbial remediation and phytoremediation for atrazine-contaminated soil and sediment has wide application prospects. Although other remediation technologies such as advanced oxidation processes (AOPs) can also efficiently remove atrazine from soil, some potential problems still need to be further clarified. Finally, some related challenges and prospects are proposed.

The high salinity of kitchen wastewater might have adverse effects on the production of short-chain fatty acids (SCFAs) in anaerobic fermentation. The effects and mechanisms of salinity on SCFA production in the anaerobic fermentation of kitchen wastewater were studied by varying the salt concentration, as follows: 0 g/L (S0), 2 g/L (S2), 6 g/L (S6), 10 g/L (S10), 15 g/L (S15), and 20 g/L (S20). Experimental results showed that hypersaline conditions (10 g NaCl/L) accelerated the release of soluble proteins at the initial stage of anaerobic fermentation. They also significantly prohibited the hydrolysis and degradation of soluble proteins and carbohydrates. Compared with low salinity tests, the SCFA concentrations under hypersaline conditions (10 g NaCl/L) only reached approximately 43% of the highest concentration on day 10, although the SCFA concentrations in all tests were very close on day 10 (14 g COD/L). High salinity delayed the production of n-butyric acid but did not change the composition of the total SCFAs. High salinity enriched Enterococcus and Bifidobacterium, the relative abundance levels of which reached 27.57% and 49.71%, respectively, before the depletion of substrate. High salinity showed a negative correlation with the relative abundance of the genera Clostridium_sensu_stricto_1, Prevotella and unclassified_f_Oscillospiraceae which are responsible for SCFA production. This study provided a theoretical basis for the fficient utilization of kitchen wastewater.

Anaerobic fermentation of corn straw with rumen microorganisms as inoculum to produce volatile fatty acids (VFAs) is important for biomass valorization. In this study, dynamic variation in bacterial and fungal community composition, carbohydrate-active enzymes (CAZymes) and key functional genes related with VFA production was explored via metagenomic sequencing. Rumen microorganisms efficiently hydrolyzed and acidified corn straw, and VFA concentration reached 8.99 g/L in 72 h. Bacterial and fungal community significantly changed, but the core genera kept stable. Low pH and VFA accumulation were the main factors affecting bacterial and fungal communities. The positive correlations between bacteria were more complex than those between fungi. Most CAZyme abundance significantly decreased after 72 h fermentation, and functional gene abundance participating in VFA generation also decreased. This study provided new insights into dynamic variation of bacteria and fungi during anaerobic ruminal fermentation in vitro, promoting the application of rumen microorganisms in practice.

In recent years, volatile fatty acid (VFA) production through anaerobic fermentation of sewage sludge, instead of methane production, has been regarded as a high-value and promising roadmap for sludge stabilization and resource recovery. This review first presents the effects of some essential factors that influence VFA production and composition. In the second part, we present an extensive analysis of conventional pretreatment and co-fermentation strategies ultimately addressed to improving VFA production and composition. Also, the effectiveness of these approaches is summarized in terms of sludge degradation, hydrolysis rate, and VFA production and composition. According to published studies, it is concluded that some pretreatments such as alkaline and thermal pretreatment are the most effective ways to enhance VFA production from sewage sludge. The possible reasons for the improvement of VFA production by different methods are also discussed. Finally, this review also highlights several current technical challenges and opportunities in VFA production with spectrum control, and further related research is proposed.

This review focuses on the research literature published in 2018 relating to thermal effects in wastewater and solid waste treatment. This review is divided into the following sections: treatment of wastewater and sludge, removal and recovery of nitrogen and phosphorus, reduction and recovery of heavy metals, membrane technology, and treatment and disposal of solid wastes. PRACTITIONER POINTS: Thermal effect plays an important role in the treatment of wastewater and sewage sludge. Recovery of nitrogen and phosphorus from wastewater and sewage sludge offers an excellent feedstock for soil amendment. Increase of treatment temperature facilitates removal and recovery of heavy metals from water and soil environment.

Due to complex inherent structure of lignocellulosic biomass, inefficient hydrolysis and acidification limits fermentative volatile fatty acids (VFA) production of mushroom residues. Meanwhile, the mushroom residues present insufficient nutrient with a high C/N ratio. To solve this issue, anaerobic co-fermentation of cellulose-rich Oyster champost and sewage sludge was tested to enhance the VFA production, and the effect of proportion of mixed substrate was investigated in this study. The results indicated that the sewage sludge yielded higher VFAs than the Oyster champost in single-substrate fermentative system. The maximal VFA yield of 595 mgCOD/gVSadded was achieved when the proportion of sewage sludge increased to 50% in the mixed substrate. In the co-fermentation system, the optimal C/N ratio and features of mixed substrate contributed to the enhancement in hydrolysis and acidification in terms of organic solubilization and VFA production, respectively. But the co-fermentation could not increase VFA/SCOD ratio, probably due to the existence of refractory products such as humic-like and protein-like materials. Besides, this co-fermentation system had strong buffer capacity and it was not necessary to dose chemicals to control the system pH for stable VFA production. Acetate was the dominant VFA product in co-fermentation systems. A modified-Logistic model fitted co-fermentation of sludge and Oyster champost well, and presented a faster rate and higher efficiency of VFA production.

Anaerobic digestion and agricultural use of sewage sludge are effective methods to treat and dispose of sewage sludge, respectively. Then, the anaerobic digested sewage sludge is applied in agricultural land and the improvement of soil properties can be expected. In this study, with the purpose of evaluating the potential of co-digestion sludge for agricultural use, plot experiments with two vegetable species (radish and Chinese cabbage) and three application dosages were carried out in a short term of six months. Focus was on soil physical properties, soil nutrient change and plant growth responses during the whole process. Results showed that application of co-digestion sludge had little effect on soil physical properties, including the bulk density, porosity, capillary porosity and non-capillary porosity. However, after the application of co-digestion sludge, the maximum increase in content of organic matter, total nitrogen, hydrolysable nitrogen, total phosphorus and available phosphorus in soil reached 51%, 125%, 212%, 15% and 87%, respectively, which supplied the available nutrients quickly and continuously. The application of co-digestion sludge promoted the growth of radish and Chinese cabbage, which was observed through increase of the leaf, root biomass and plants height. Consequently, co-digestion sludge has a good application prospect for improving soil productivity as fertilizer.

Single pretreatment of sewage sludge, either physical, chemical or biological, has its own drawbacks in term of poor sanitization, energy intensity and high operational and capital cost. To tackle these drawbacks, combined high pressure homogenization (HPH) and free nitrous acid (FNA) pretreatment for sludge solubilization and further biodegradation in anaerobic digestion was investigated. Synergistic effect of combined HPH (40 MPa) and FNA (2.49 mg/L) pretreatment (HPH-FNA) for improving anaerobic digestion was evaluated, and its effect on archaeal and bacterial community structure was analyzed. Compared with single HPH and FNA pretreatments, HPH-FNA pretreatment efficiently solubilized wasted activated sludge (WAS), subsequently improved anaerobic digestion. Cumulative biogas production from sewage sludge pretreated with HPH-FNA was 154%, 108% and 284% more than that with single pretreatment of FNA, HPH and raw sludge, respectively. In addition, volumetric biogas production of combined pretreatment system (815 ml) was more than the sum from single pretreatment (710 ml). Methane content in biogas for raw sludge, FNA, HPH and HPH-FNA pretreated sludge was 45%, 51%, 55% and 65%, respectively. Illumina MiSeq sequencing analysis revealed that HPH-FNA pretreatment promoted bacterial growth of phyla Bacteroidetes, Firmicutes and Synergistetes and archaeal genera Methanospirillum and Methanosaeta. Overall, combined HPH-FNA pretreatment of sewage sludge, prior to anaerobic digestion, is an environmentally-friendly and potentially economic technology.

Rumen microorganisms have the ability to efficiently hydrolyze and acidify lignocellulosic biomass. The effectiveness of long-term rumen microorganism fermentation of lignocellulose in vitro for producing volatile fatty acids (VFAs) is unclear. The feasibility of long-term rumen microorganism fermentation of lignocelluose was evaluated in this study, and a stable VFA production was successfully realized for 120 d. Results showed that VFA concentration reached to 5.32-8.48 g/L during long-term fermentation. Hydrolysis efficiency of hemicellulose and cellulose reached 36.5%-52.2% and 29.4%-38.4%, respectively. A stable bacterial community was mainly composed of Prevotella, Rikenellaceae_RC9_gut_group, Ruminococcus, and Succiniclasticum. VFA accumulation led to a pH decrease, which caused the change of bacterial community structure. Functional prediction showed that the functional genes related to hydrolysis and acidogenesis of corn stover were highly expressed during long-term fermentation. The successful long-term rumen fermentation to produce VFAs is of great significance for the practical application of rumen microorganisms.

Nutrients carried in upstream rivers to lakes are the main cause of eutrophication. Building near-natural estuarine wetlands between rivers and lakes is an effective way to remove pollutants and restore the ecology of estuarine areas. However, for the existing estuarine wetland ecological restoration projects, there is a lack of corresponding evaluation methods and index systems to make a comprehensive assessment of their restoration effects. By summarizing a large amount of literature and doing field research, an index system was constructed by combining the characteristics of the near-natural estuarine wetlands themselves. It covered environmental benefits, technical management and maintenance, and socio-economic functions, and contained 3 systems, 7 criteria, and 16 indicators. The analytical hierarchy process (AHP) was used to determine the weights of each indicator. The top 5 indicators in order of importance were habitat diversity, total phosphorus (TP), coverage of aquatic plants, ammonia nitrogen (NH3-N), and adaptation to the surrounding landscape. The above evaluation system was used for the comprehensive evaluation of the water purification project in the Fuhe estuarine wetland, Hebei Province, as an example. The results showed that the comprehensive score of the Fuhe estuarine wetland at this stage was 4.1492, and the evaluation grade was excellent. The effect of water purification and ecological restoration was good, and the selected technology was suitable and stable in operation. It had a greater positive impact on the surrounding economy and society and can be promoted and applied. The research results were important for clarifying the advantages and defects of the project and developing efficient and advanced restoration technologies.

Microplastics exist widely in water environment. The microplastic distribution in sediments can better reflect the long-term microplastic pollution, especially the vertical distribution. However, the vertical microplastic distribution in sediments is diverse and unclear. This paper is the first study on vertical microplastic distribution in estuary sediment of Fuhe River, the main upstream river flowing to Baiyangdian Wetland in the northern China. The typical feature of Fuhe River is that the effluent of municipal wastewater treatment plants is its main water source. Microplastics in 15 sampling sites and different depths (0–50 cm) were examined. Results showed that the microplastic content decreased with the increase of sediment depth, and the highest content was 1049 ± 462 items/kg in the topmost sediment layer (0–5 cm). The particle size of microplastics was smaller in deeper sediment layers. The proportion of colored microplastics in deeper sediment layers was larger than that in shallower layers. Polyethylene (PE) and polypropylene (PP) were the main plastic polymer types in all sediment samples. The spatial distribution characteristics of microplastics in sediments were closely related to human activities, and the microplastic content was higher in the areas with more intense human activities. This study is helpful to understand the detailed distribution characteristics of microplastics in typical rivers in the northern China, and can provide guidance for reducing microplastic pollution.

The estuarine wetland buffer zone of the Fuhe river plays an important role in terms of improving water quality of Baiyangdian lake. In this study, the water purification effect and microbial community composition of an estuarine wetland buffer zone during a low-temperature period were investigated (March, 2021). The results indicated that the pre-sedimentation ecological pond (Pep), the subsurface flow wetland (Sfw), and the aquatic plant pond (App) exhibited good removal effects on total phosphorus (TP) and NH3-N. In contrast, the removal effects on chemical oxygen demand (COD) and total nitrogen (TN) was unsatisfied. Various phosphorus removal bacteria were detected in the water samples from the Pep and the Sfw, such as Arthrobacter, Flavobacterium, and Planococcus. These findings indicated the water purification performance and the microbial community composition in an estuarine wetland buffer zone during a low-temperature period.

Water shortage severely restricts vegetation restoration of mining area in the northwest China. Moderate treatment of reclaimed water is essential for improving the local ecological environment. In this study, relevant water quality standards issued by the states and research results were comprehensively considered to propose a reclaimed water quality standard suitable for vegetation restoration of mining area. The available domestic sewage and mine water was moderately treated by hybrid biological reactor system and nanofiltration membrane system, respectively. The effluent quality meet the requirement of reclaimed water quality standards in this study. This study provides theoretical support for vegetation restoration of mining area.

Rumen liquid is excellent to effectively degrade lignocellulose. In this study, the suitable rice straw load during anaerobic fermentation of rice straw with rumen liquid as inoculum was explored to improve volatile fatty acid (VFA) production. At 10.0% rice straw load, the highest VFA concentration reached 10821.4 mg/L, and acetic acid and propionic acid were the main components. In 10.0% rice straw load system, high concentration of soluble chemical oxygen demand (SCOD) was also observed, and the enzymatic activities at 48 h were higher than those at other rice straw loads. At 10.0% rice straw load, lower diversity and richness of rumen bacteria were found than those at other rice straw loads. Bacteroides, Prevotella, and Ruminococcus were the main rumen bacteria during rice straw degradation, and the rumen bacteria might secret effective lignocellulolytic enzymes to enhance the hydrolysis and acidogenesis of rice straw. The determination of suitable rice straw load will be beneficial to the application of rumen liquid as inoculum in actual production.

Properly increasing mobility of heavy metals could promote phytoremediation of contaminated soil. Fe

In order to prolong the effect of CaO2 on improvement of short-chain fatty acids (SCFAs) production in sludge anaerobic fermentation, CaO2 particles were successfully loaded onto the porous surface of steel slag pre-modified with salicylic acid-methanol (SAM-SS). The prepared CaO2/SAM-SS was then characterized and investigated for its effects on anaerobic fermentation. Experimental results revealed that, due to the slow release and reaction of CaO2/SAM-SS, SCFAs concentrations in CaO2/SAM-SS tests were significantly higher than in the control and SAM-SS tests, and high SCFAs concentration was sustained for a longer period than in the CaO2 tests. Since most bacterial indexes were reduced by CaO2/SAM-SS, more supply of "raw materials" from a better disintegration and hydrolysis, which was associated with the alkalinity and •OH radicals released from the reaction of CaO2 with H2O, contribute to the higher SCFAs yields. This study provides a new approach towards a higher and longer SCFAs harvesting.

This review paper focuses on the researches published in 2019 in the field of thermal effects in wastewater and solid waste treatment. The content of this review paper includes five parts: wastewater and sludge treatment, nutrient removal and recovery, membrane technology, heavy metal removal and immobilization, and organic waste utilization. © 2020 Water Environment Federation PRACTITIONER POINTS: Thermal effect plays an important role in treatment of wastewater and sewage sludge. Recovery of nitrogen and phosphorus from wastewater and sewage sludge reduces environmental pollution and offers new products. Temperature improves removal and recovery of heavy metals and organic wastes.

Coal is the most abundant fossil fuel in the world and its combustion accompanies with the emission of SOX, which is responsible for serious environmental problems. To reduce the emission of SOX is essential for clean fuel. In the present study, indigenous microorganisms acclimatized from fat coal itself and exotic microorganisms from sewage sludge were used for coal biodesulfurization. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectral analysis were used to investigate the structural change of fat coal during the bioleaching. Results showed that kaolinite and quartz were the main minerals, and pyrite was the main inorganic sulfide in fat coal. After 36-day biodesulfurization, the total sulfur in fat coal decreased from 5.07% to 2.77% and 2.75%, and the pyritic removal was 77.68% and 87.88% with indigenous and exotic microorganisms, respectively. The exotic microorganisms were more effective to oxidize the pyrite than the indigenous microorganisms. But both microorganisms did not interact with the organic sulfur, which existed as C S bond in fat coal. FT-IR analyses showed the damage of kaolinite structure, indicating potential microbial effect on kaolinite, which has not been reported before. Raman spectral analysis was for the first time used to investigate the changes of coal macromolecular structure during the bioleaching. FT-IR and Raman spectra confirmed the changes of aromatic C H, O H bonds, which led to the increase of carbon crystallinity index.

In this study, feasibility, optimization, and mechanisms of enzyme pretreatment to enhance anaerobic digestion of corn stover were investigated. Results showed that the enzyme pretreatment efficiently enhanced the biogas yield, and the optimal conditions of enzyme pretreatment were an enzyme load of 30 FPU/g, a pretreatment time of 24 h, and a solid content of 60 g/L. Under the optimal conditions, the cumulative biogas yield increased by 36.9%, which was mainly attributed to disruption of surface structure and degradation of noncrystalline cellulose in the enzyme-pretreated corn stover. The kinetic analysis indicated that enzyme pretreatment significantly enhanced the hydrolysis rate and biogas production rate to 0.15/d and 23.89 mL/gVS, and shortened the lag phase time to 1.2 d. Correlation analysis illustrated that the SCOD yield of 250-350 mg/g from corn stover after enzyme pretreatment was suitable for the further anaerobic digestion of corn stover.