104 results on '"Panwar B"'
Search Results
2. Tokapal tuff-facies kimberlite, Bastar craton, Central India: A nickel prospect?
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Chalapathi Rao, N. V., Lehmann, Bernd, Panwar, B. K., Kumar, Alok, and Mainkar, Datta
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- 2013
- Full Text
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3. Diamond-facies chrome spinel from the Tokapal kimberlite, Indrāvati basin, central India and its petrological significance
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Chalapathi Rao, N. V., Lehmann, B., Mainkar, D., and Panwar, B. K.
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- 2012
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4. Adiposity and risk of decline in glomerular filtration rate : Meta-analysis of individual participant data in a global consortium
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Chang AR, Grams ME, Ballew SH, Bilo H, Correa A, Evans M, Gutierrez OM, Hosseinpanah F, Iseki K, Kenealy T, Klein B, Kronenberg F, Lee BJ, Li Y, Miura K, Navaneethan SD, Roderick PJ, Valdivielso JM, Visseren FLJ, Zhang L, Gansevoort RT, Hallan SI, Levey AS, Matsushita K, Shalev V, Woodward M, Astor B, Appel L, Greene T, Chen T, Chalmers J, Arima H, Perkovic V, Yatsuya H, Tamakoshi K, Hirakawa Y, Coresh J, Sang Y, Polkinghorne K, Chadban S, Atkins R, Levin A, Djurdjev O, Klein R, Lee K, Liu L, Zhao M, Wang F, Wang J, Tang M, Heine G, Emrich I, Zawada A, Bauer L, Nally J, Schold J, Shlipak M, Sarnak M, Katz R, Hiramoto J, Iso H, Yamagishi K, Umesawa M, Muraki I, Fukagawa M, Maruyama S, Hamano T, Hasegawa T, Fujii N, Jafar T, Hatcher J, Poulter N, Chaturvedi N, Wheeler D, Emberson J, Townend J, Landray M, Brenner H, Schöttker B, Saum KU, Rothenbacher D, Fox C, Hwang SJ, Köttgen A, Schneider MP, Eckardt KU, Green J, Kirchner HL, Ito S, Miyazaki M, Nakayama M, Yamada G, Cirillo M, Romundstad S, Øvrehus M, Langlo KA, Irie F, Sairenchi T, Rebholz CM, Young B, Boulware LE, Ishikawa S, Yano Y, Kotani K, Nakamura T, Jee SH, Kimm H, Mok Y, Chodick G, Wetzels JFM, Blankestijn PJ, van Zuilen AD, Bots M, Inker L, Peralta C, Kollerits B, Ritz E, Nitsch D, Fletcher A, Bottinger E, Nadkarni GN, Ellis SB, Nadukuru R, Fernandez E, Betriu A, Bermudez-Lopez M, Stengel B, Metzger M, Flamant M, Houillier P, Haymann JP, Froissart M, Ueshima H, Okayama A, Tanaka S, Okamura T, Elley CR, Collins JF, Drury PL, Ohkubo T, Asayama K, Metoki H, Kikuya M, Iseki C, Nelson RG, Knowler WC, Bakker SJL, Heerspink HJL, Brunskill N, Major R, Shepherd D, Medcalf J, Jassal SK, Bergstrom J, Ix JH, Barrett-Connor E, Kovesdy C, Kalantar-Zadeh K, Sumida K, Muntner P, Warnock D, Judd S, Panwar B, de Zeeuw D, Brenner B, Sedaghat S, Ikram MA, Hoorn EJ, Dehghan A, Wong TY, Sabanayagam C, Cheng CY, Banu R, Segelmark M, Stendahl M, Schön S, Tangri N, Sud M, Naimark D, Wen CP, Tsao CK, Tsai MK, Chen CH, Konta T, Hirayama A, Ichikawa K, Hadaegh F, Mirbolouk M, Azizi F, Solbu MD, Jenssen TG, Eriksen BO, Eggen AE, Lannfelt L, Larsson A, Ärnlöv J, Landman GWD, van Hateren KJJ, Kleefstra N, Chen J, Kwak L, Surapaneni A., Chang, Ar, Grams, Me, Ballew, Sh, Bilo, H, Correa, A, Evans, M, Gutierrez, Om, Hosseinpanah F, Iseki K, Kenealy, T, Klein, B, Kronenberg, F, Lee, Bj, Li, Y, Miura, K, Navaneethan, Sd, Roderick, Pj, Valdivielso, Jm, Visseren, Flj, Zhang, L, Gansevoort, Rt, Hallan, Si, Levey, A, Matsushita, K, Shalev, V, Woodward, M, Astor, B, Appel, L, Greene, T, Chen, T, Chalmers, J, Arima, H, Perkovic, V, Yatsuya, H, Tamakoshi, K, Hirakawa, Y, Coresh, J, Sang, Y, Polkinghorne, K, Chadban, S, Atkins, R, Levin, A, Djurdjev, O, Klein, R, Lee, K, Liu, L, Zhao, M, Wang, F, Wang, J, Tang, M, Heine, G, Emrich, I, Zawada, A, Bauer, L, Nally, J, Schold, J, Shlipak, M, Sarnak, M, Katz, R, Hiramoto, J, Iso, H, Yamagishi, K, Umesawa, M, Muraki, I, Fukagawa, M, Maruyama, S, Hamano, T, Hasegawa, T, Fujii, N, Jafar, T, Hatcher, J, Poulter, N, Chaturvedi, N, Wheeler, D, Emberson, J, Townend, J, Landray, M, Brenner, H, Schöttker, B, Saum, Ku, Rothenbacher, D, Fox, C, Hwang, Sj, Köttgen, A, Schneider, Mp, Eckardt, Ku, Green, J, Kirchner, Hl, Ito, S, Miyazaki, M, Nakayama, M, Yamada, G, Cirillo, M, Romundstad, S, Øvrehus, M, Langlo, Ka, Irie, F, Sairenchi, T, Rebholz, Cm, Young, B, Boulware, Le, Ishikawa, S, Yano, Y, Kotani, K, Nakamura, T, Jee, Sh, Kimm, H, Mok, Y, Chodick, G, Wetzels, Jfm, Blankestijn, Pj, van Zuilen, Ad, Bots, M, Inker, L, Peralta, C, Kollerits, B, Ritz, E, Nitsch, D, Fletcher, A, Bottinger, E, Nadkarni, Gn, Ellis, Sb, Nadukuru, R, Fernandez, E, Betriu, A, Bermudez-Lopez, M, Stengel, B, Metzger, M, Flamant, M, Houillier, P, Haymann, Jp, Froissart, M, Ueshima, H, Okayama, A, Tanaka, S, Okamura, T, Elley, Cr, Collins, Jf, Drury, Pl, Ohkubo, T, Asayama, K, Metoki, H, Kikuya, M, Iseki, C, Nelson, Rg, Knowler, Wc, Bakker, Sjl, Heerspink, Hjl, Brunskill, N, Major, R, Shepherd, D, Medcalf, J, Jassal, Sk, Bergstrom, J, Ix, Jh, Barrett-Connor, E, Kovesdy, C, Kalantar-Zadeh, K, Sumida, K, Muntner, P, Warnock, D, Judd, S, Panwar, B, de Zeeuw, D, Brenner, B, Sedaghat, S, Ikram, Ma, Hoorn, Ej, Dehghan, A, Wong, Ty, Sabanayagam, C, Cheng, Cy, Banu, R, Segelmark, M, Stendahl, M, Schön, S, Tangri, N, Sud, M, Naimark, D, Wen, Cp, Tsao, Ck, Tsai, Mk, Chen, Ch, Konta, T, Hirayama, A, Ichikawa, K, Hadaegh, F, Mirbolouk, M, Azizi, F, Solbu, Md, Jenssen, Tg, Eriksen, Bo, Eggen, Ae, Lannfelt, L, Larsson, A, Ärnlöv, J, Landman, Gwd, van Hateren, Kjj, Kleefstra, N, Chen, J, Kwak, L, Surapaneni, A., Lifestyle Medicine (LM), Cardiovascular Centre (CVC), Groningen Kidney Center (GKC), Groningen Institute for Organ Transplantation (GIOT), Real World Studies in PharmacoEpidemiology, -Genetics, -Economics and -Therapy (PEGET), Asayama, Kei, and Sedaghat, SeyyedMah
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CHRONIC KIDNEY-DISEASE ,Male ,030232 urology & nephrology ,030204 cardiovascular system & hematology ,OBESITY PARADOX ,Body Mass Index ,BMI, eGFR, CKD-PC ,Cohort Studies ,0302 clinical medicine ,Risk Factors ,Urologi och njurmedicin ,Medicine ,ALL-CAUSE MORTALITY ,Adiposity ,2. Zero hunger ,Aged, 80 and over ,Medicine(all) ,education.field_of_study ,Hazard ratio ,ASSOCIATION ,General Medicine ,Middle Aged ,3. Good health ,Cohort ,Female ,Waist Circumference ,Life Sciences & Biomedicine ,WAIST CIRCUMFERENCE ,Obesity paradox ,Glomerular Filtration Rate ,Adult ,Waist ,Population ,Renal function ,03 medical and health sciences ,Medicine, General & Internal ,General & Internal Medicine ,CKD ,Urology and Nephrology ,Humans ,Mortality ,education ,Aged ,Science & Technology ,business.industry ,Research ,medicine.disease ,Body Height ,BODY-MASS INDEX ,Kidney Failure, Chronic ,WEIGHT ,Renal disorders Radboud Institute for Health Sciences [Radboudumc 11] ,business ,Body mass index ,Demography ,Kidney disease - Abstract
ObjectiveTo evaluate the associations between adiposity measures (body mass index, waist circumference, and waist-to-height ratio) with decline in glomerular filtration rate (GFR) and with all cause mortality.DesignIndividual participant data meta-analysis.SettingCohorts from 40 countries with data collected between 1970 and 2017.ParticipantsAdults in 39 general population cohorts (n=5 459 014), of which 21 (n=594 496) had data on waist circumference; six cohorts with high cardiovascular risk (n=84 417); and 18 cohorts with chronic kidney disease (n=91 607).Main outcome measuresGFR decline (estimated GFR decline ≥40%, initiation of kidney replacement therapy or estimated GFR 2) and all cause mortality.ResultsOver a mean follow-up of eight years, 246 607 (5.6%) individuals in the general population cohorts had GFR decline (18 118 (0.4%) end stage kidney disease events) and 782 329 (14.7%) died. Adjusting for age, sex, race, and current smoking, the hazard ratios for GFR decline comparing body mass indices 30, 35, and 40 with body mass index 25 were 1.18 (95% confidence interval 1.09 to 1.27), 1.69 (1.51 to 1.89), and 2.02 (1.80 to 2.27), respectively. Results were similar in all subgroups of estimated GFR. Associations weakened after adjustment for additional comorbidities, with respective hazard ratios of 1.03 (0.95 to 1.11), 1.28 (1.14 to 1.44), and 1.46 (1.28 to 1.67). The association between body mass index and death was J shaped, with the lowest risk at body mass index of 25. In the cohorts with high cardiovascular risk and chronic kidney disease (mean follow-up of six and four years, respectively), risk associations between higher body mass index and GFR decline were weaker than in the general population, and the association between body mass index and death was also J shaped, with the lowest risk between body mass index 25 and 30. In all cohort types, associations between higher waist circumference and higher waist-to-height ratio with GFR decline were similar to that of body mass index; however, increased risk of death was not associated with lower waist circumference or waist-to-height ratio, as was seen with body mass index.ConclusionsElevated body mass index, waist circumference, and waist-to-height ratio are independent risk factors for GFR decline and death in individuals who have normal or reduced levels of estimated GFR.
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- 2019
5. Distribution Of Cadmium And Nickel Among Various Forms In Natural And Contaminated Soils Amended With Edta
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Panwar, B. S., Ahmed, K. S., Sihag, D., and Patel, A. L.
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- 2005
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6. Cadmium Uptake by Cowpea and Mungbean as Affected by Cd and P Application
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Panwar, B. S., Singh, J. P., and Laura, R. D.
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- 1999
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7. CD4+ follicular helper-like T cells are key players in anti-tumor immunity
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Singh, D, primary, Ganesan, AP, additional, Panwar, B, additional, Eschweiler, S, additional, Hanley, CJ, additional, Madrigal, A, additional, Ramírez-Suástegui, C, additional, Wang, A, additional, Clarke, J, additional, Wood, O, additional, Garrido-Martin, EM, additional, Chee, SJ, additional, Seumois, G, additional, Belanger, S, additional, Alzetani, A, additional, Woo, E, additional, Friedmann, PS, additional, Crotty, S, additional, Thomas, GJ, additional, Sanchez-Elsner, T, additional, Ay, F, additional, Ottensmeier, CH, additional, and Vijayanand, P, additional
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- 2020
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8. PHYTOREMEDIATION OF NICKEL-CONTAMINATED SOILS BY BRASSICA SPECIES
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Panwar, B. S., Ahmed, K. S., and Mittal, S. B.
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- 2002
9. Equine Husbandry & Equestrian Sports
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Panwar, B. S., Yadav, K. N., Panwar, B. S., and Yadav, K. N.
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- Horse sports, Horses
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Description based on print version record.
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- 2010
10. Soil-root interactions in potassium supply
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Végh, Krisztina, primary, Csillag, J., additional, Lukács, A., additional, Panwar, B, additional, and Füleky, Gy., additional
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- 2014
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11. Inverse temperature dependence of reverse gate leakage current in AlGaN/GaN HEMT
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Kaushik, J K, primary, Balakrishnan, V R, additional, Panwar, B S, additional, and Muralidharan, R, additional
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- 2012
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12. Theoretical and experimental analysis of high Q SAW resonator transient response in a wireless sensor interrogation application
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Varshney, P., primary, Panwar, B. S., additional, Rathore, P., additional, Ballandras, S., additional, Francois, B., additional, Martin, G., additional, Friedt, J.-M., additional, and Retornaz, T., additional
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- 2012
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13. Phytoremediation: Enhanced cadmium (Cd) accumulation by organic manuring, EDTA and microbial inoculants (Azotobacter sp., Pseudomonas sp.) in Indian mustard (Brassica juncea L.)
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Panwar, B., primary, Kádár, I., additional, Bíró, B., additional, Rajkai-Végh, K., additional, Ragályi, P., additional, Rékási, M., additional, and Márton, L., additional
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- 2011
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14. High sensitivity MEMS capacitive tilt sensor using multi-resonant cylindrical waveguide structure
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Panwar, B. S., primary and Panwar, Siddharth, additional
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- 2011
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15. Phytoremediation: A novel green technology to restore soil health
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Panwar, B., primary, Marton, L., additional, Kádár, I., additional, Anton, A., additional, and Németh, T., additional
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- 2010
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16. Optimization of Ge-Dose and Doping in Base of SiGe HBTs
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Khanduri, Gagan, primary and Panwar, B. S., additional
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- 2007
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17. An Iteration Approach for Optimization of Base Doping Profile in Si BJTs
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Khanduri, Gagan, primary and Panwar, B. S., additional
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- 2007
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18. Kinetics of cadmium in different Indian and Hungarian soils: Incubation study at field capacity
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Panwar, B., primary, Grewal, M., additional, and Márton, L., additional
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- 2007
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19. Study of Cutoff Frequency at High Collector Current Density in SiGe Single-Heterojunction Bipolar Transistor
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Khanduri, G. M., additional and Panwar, B. S., additional
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- 2004
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20. Simultaneous optimization of doping profile and Ge-dose in base in SiGe HBTs.
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Khanduri, G. and Brishbhan Panwar, B.
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- 2007
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21. Current gain modeling of SiGe DHBTs in SPICE including retarding potential barrier effect.
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Khanduri, G. and Panwar, B.
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- 2007
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22. Base doping profile optimization including carriers velocity saturation effect.
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Khanduri, G. and Panwar, B.
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- 2007
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23. IMS SIP core server test bed.
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Panwar, B. and Singh, K.
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- 2007
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24. Characteristics of surface acoustic wave convolver in the monolithic metal–zinc oxide–silicon nitride–silicon dioxide–silicon structure
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Panwar, B. S., primary
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- 2002
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25. Use of second order information in the auto regressive moving average technique for the design of linear phase SAW filters.
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Panwar, B., Priyanka, and Joshi, S.
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- 2005
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26. An Improved Transducer Configuration for Phase-Coded Surface Acoustic Wave Devices
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Majumder, Swati, primary and Panwar, B S, additional
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- 1992
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27. Rapid thermal anneal of hydrogen-implanted metal-silicon nitride-silicon dioxide-silicon structure
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Panwar, B. S., primary and Bhattacharyya, A. B., additional
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- 1990
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28. Phytoremediation: Enhanced cadmium (Cd) accumulation by organic manuring, EDTA and microbial inoculants (Azotobactersp., Pseudomonassp.) in Indian mustard (Brassica junceaL.)
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Panwar, B., Kádár, I., Bíró, B., Rajkai-Végh, K., Ragályi, P., Rékási, M., and Márton, L.
- Abstract
Phytoremediation is an approach designed to extract excessive heavy metals from contaminated soils through plant uptake. Cadmium (Cd) is among the elements most toxic to living organisms. Health hazards associated with the lethal intake of Cd include renal (kidney) damage, anaemia, hypertension and liver damage. A greenhouse experiment was carried out with Indian mustard (Brassica juncea) grown on artificially spiked soil (100 μg Cd g−1) with EDTA (2 mmol kg−1in 5 split doses), FYM, vermicompost (VC) and microbial inoculants (MI) such as Azotobactersp. and Pseudomonassp. The growth of Brassica junceaL. was better in soil amended with FYM or VC as compared to unamended Cd-polluted soil. Growth was slightly suppressed in EDTA-treated soil, whereas it was better after treatment with MI. The application of FYM and VC increased the dry matter yield of Indian mustard either alone or in combination with microbial inoculants, while that of EDTA caused a significant decrease in the biomass of Indian mustard. The application of microbial inoculants increased the dry matter yield of both the roots and shoots, but not significantly, because MI shows greater sensitivity towards cadmium. The maximum cadmium concentration was observed in the EDTA +MI treatment, but Cd uptake was maximum in the VC + MI treatment. The Cd concentration in the shoots increased by 120% in CdEDTAover the Cd100treatment, followed by CdVC(65%) and CdFYM(42%) in the absence of microbial inoculants. The corresponding values in the presence of MI were 107, 51 and 37%, respectively. A similar trend was also observed in the roots in the order CdEDTA+M> CdVC+M> CdFYM+M>Cd100+M.MI caused an increase in Cd content of 5.5% in the roots and 4.1% in the shoots in the CdEDTA+Mtreatment compared with the CdEDTAtreatment. FYM, VC and EDTA also increased Cd uptake significantly both in the shoots and roots with and without microbial inoculants.The results indicated that Vermicompost in combination with microbial inoculants is the best treatment for the phytoremediation of Cd-contaminated soil by Indian mustard, as revealed by the Cd uptake values in the shoots: CdVC+M(2265.7 μg/pot) followed by CdEDTA+M(2251.2 μg/pot), CdFYM+M(1485.7 μg/pot) and Cd100+M(993.1 μg/pot).
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- 2011
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29. Nutrient intake of rural pregnant women of Haryana state, northern India: relationship between income and education.
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Panwar, Bharti, Punia, Darshan, Panwar, B, and Punia, D
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PREGNANT women ,NUTRITION in pregnancy ,DIET ,INCOME ,INGESTION ,NUTRITION policy ,PREGNANCY ,PRENATAL care ,DIETARY proteins ,RURAL health ,SURVEYS ,MICRONUTRIENTS ,EDUCATIONAL attainment - Abstract
The daily nutrient intake of 90 pregnant women from farming and non-farming communities in six rural villages of Haryana State, Northern India have been determined. As a result of questionnaires and interviews, nutrient intake for 3 consecutive days were calculated. Mean daily intakes of farming and non-farming pregnant women examined in this study were lower for energy, calcium and iron than the recently prescribed Indian recommended dietary allowances (RDAs). Protein intake of non-farming women was significantly lower and that of farming women was almost similar to RDA. Intake of fat by pregnant women was double the RDA. The mean daily intakes of thiamine, riboflavin and niacin by women of both the communities were found to be adequate. The diets of pregnant women could meet half the requirement of folic acid and even less than half for ascorbic acid. Income of pregnant women did not show any influence on nutrient intakes but educational level of women certainly reflected differences in vitamin intakes. [ABSTRACT FROM AUTHOR]
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- 1998
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30. Food intake of rural pregnant women of Haryana State, northern India: relationship with education and income.
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Panwar, Bharti, Punia, Darshan, Panwar, B, and Punia, D
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NUTRITION in pregnancy ,PREGNANT women ,RURAL conditions ,HEALTH - Abstract
Average daily food intakes of 90 rural pregnant women of farming and non-farming communities of Northern India were determined. As a result of questionnaires and interviews, food intakes for three consecutive days were collected. Intakes of cereals, pulses, roots and tubers, and sugar and jaggery, by both farming and non-farming females were significantly lower than the prescribed Indian recommended dietary intakes (RDI). The consumption of milk and milk products and fats and oils was adequate whereas green leafy vegetables and fruits were the most limited food items. No significant overall differences were observed between females from farming and non-farming backgrounds. Level of education did not show significant influence on intakes of different food items. The consumption of fruits and green leafy vegetables increased with the increase in family monthly income. There is a pressing need to educate rural pregnant women regarding their increased nutritional requirements. [ABSTRACT FROM AUTHOR]
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- 1998
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31. Soil Test Fertilizer Recommendations Increase Economic Yields of Rice
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Sharma, J. C., Karwasra, S. P., Sharma, A. P., and Panwar, B. S.
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Soil Test Fertilizer ,Economic Yields - Abstract
This article 'Soil Test Fertilizer Recommendations Increase Economic Yields of Rice' appeared in the International Rice Research Newsletter series, created by the International Rice Research Institute (IRRI). The primary objective of this publication was to expedite communication among scientists concerned with the development of improved technology for rice and for rice based cropping systems. This publication will report what scientists are doing to increase the production of rice in as much as this crop feeds the most densely populated and land scarce nations in the world.
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- 1989
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32. Prediction of vitamin interacting residues in a vitamin binding protein using evolutionary information
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Panwar Bharat, Gupta Sudheer, and Raghava Gajendra P S
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Vitamin-interacting residue ,Pyridoxal-5-phosphate ,SVM ,PSSM ,VitaPred ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background The vitamins are important cofactors in various enzymatic-reactions. In past, many inhibitors have been designed against vitamin binding pockets in order to inhibit vitamin-protein interactions. Thus, it is important to identify vitamin interacting residues in a protein. It is possible to detect vitamin-binding pockets on a protein, if its tertiary structure is known. Unfortunately tertiary structures of limited proteins are available. Therefore, it is important to develop in-silico models for predicting vitamin interacting residues in protein from its primary structure. Results In this study, first we compared protein-interacting residues of vitamins with other ligands using Two Sample Logo (TSL). It was observed that ATP, GTP, NAD, FAD and mannose preferred {G,R,K,S,H}, {G,K,T,S,D,N}, {T,G,Y}, {G,Y,W} and {Y,D,W,N,E} residues respectively, whereas vitamins preferred {Y,F,S,W,T,G,H} residues for the interaction with proteins. Furthermore, compositional information of preferred and non-preferred residues along with patterns-specificity was also observed within different vitamin-classes. Vitamins A, B and B6 preferred {F,I,W,Y,L,V}, {S,Y,G,T,H,W,N,E} and {S,T,G,H,Y,N} interacting residues respectively. It suggested that protein-binding patterns of vitamins are different from other ligands, and motivated us to develop separate predictor for vitamins and their sub-classes. The four different prediction modules, (i) vitamin interacting residues (VIRs), (ii) vitamin-A interacting residues (VAIRs), (iii) vitamin-B interacting residues (VBIRs) and (iv) pyridoxal-5-phosphate (vitamin B6) interacting residues (PLPIRs) have been developed. We applied various classifiers of SVM, BayesNet, NaiveBayes, ComplementNaiveBayes, NaiveBayesMultinomial, RandomForest and IBk etc., as machine learning techniques, using binary and Position-Specific Scoring Matrix (PSSM) features of protein sequences. Finally, we selected best performing SVM modules and obtained highest MCC of 0.53, 0.48, 0.61, 0.81 for VIRs, VAIRs, VBIRs, PLPIRs respectively, using PSSM-based evolutionary information. All the modules developed in this study have been trained and tested on non-redundant datasets and evaluated using five-fold cross-validation technique. The performances were also evaluated on the balanced and different independent datasets. Conclusions This study demonstrates that it is possible to predict VIRs, VAIRs, VBIRs and PLPIRs from evolutionary information of protein sequence. In order to provide service to the scientific community, we have developed web-server and standalone software VitaPred (http://crdd.osdd.net/raghava/vitapred/).
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- 2013
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33. Analysis and prediction of cancerlectins using evolutionary and domain information
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Chauhan Jagat S, Panwar Bharat, Kumar Ravi, and Raghava Gajendra PS
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Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Background Predicting the function of a protein is one of the major challenges in the post-genomic era where a large number of protein sequences of unknown function are accumulating rapidly. Lectins are the proteins that specifically recognize and bind to carbohydrate moieties present on either proteins or lipids. Cancerlectins are those lectins that play various important roles in tumor cell differentiation and metastasis. Although the two types of proteins are linked, still there is no computational method available that can distinguish cancerlectins from the large pool of non-cancerlectins. Hence, it is imperative to develop a method that can distinguish between cancer and non-cancerlectins. Results All the models developed in this study are based on a non-redundant dataset containing 178 cancerlectins and 226 non-cancerlectins in which no two sequences have more than 50% sequence similarity. We have applied the similarity search based technique, i.e. BLAST, and achieved a maximum accuracy of 43.25%. The amino acids compositional analysis have shown that certain residues (e.g. Leucine, Proline) were preferred in cancerlectins whereas some other (e.g. Asparatic acid, Asparagine) were preferred in non-cancerlectins. It has been found that the PROSITE domain "Crystalline beta gamma" was abundant in cancerlectins whereas domains like "SUEL-type lectin domain" were found mainly in non-cancerlectins. An SVM-based model has been developed to differentiate between the cancer and non-cancerlectins which achieved a maximum Matthew's correlation coefficient (MCC) value of 0.32 with an accuracy of 64.84%, using amino acid compositions. We have developed a model based on dipeptide compositions which achieved an MCC value of 0.30 with an accuracy of 64.84%. Thereafter, we have developed models based on split compositions (2 and 4 parts) and achieved an MCC value of 0.31, 0.32 with accuracies of 65.10% and 66.09%, respectively. An SVM model based on Position Specific Scoring Matrix (PSSM), generated by PSI-BLAST, was developed and achieved an MCC value of 0.36 with an accuracy of 68.34%. Finally, we have integrated the PROSITE domain information with PSSM and developed an SVM model that has achieved an MCC value of 0.38 with 69.09% accuracy. Conclusion BLAST has been found inefficient to distinguish between cancer and non-cancerlectins. We analyzed the protein sequences of cancer and non-cancerlectins and identified interesting patterns. We have been able to identify PROSITE domains that are preferred in cancer and non-cancerlectins and thus provided interesting insights into the two types of proteins. The method developed in this study will be useful for researchers studying cancerlectins, lectins and cancer biology. The web-server based on the above study, is available at http://www.imtech.res.in/raghava/cancer_pred/
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- 2011
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34. Prediction and classification of aminoacyl tRNA synthetases using PROSITE domains
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Panwar Bharat and Raghava Gajendra PS
- Subjects
Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Aminoacyl tRNA synthetases (aaRSs) catalyse the first step of protein synthesis in all organisms. They are responsible for the precise attachment of amino acids to their cognate transfer RNAs. There are twenty different types of aaRSs, unique for each amino acid. These aaRSs have been divided into two classes, each comprising ten enzymes. It is important to predict and classify aaRSs in order to understand protein synthesis. Results In this study, all models were developed on a non-redundant dataset containing 117 aaRSs and an equal number of non-aaRSs, in which no two sequences have more than 30% similarity. First, we applied the similarity search technique, BLAST, and achieved a maximum accuracy of 67.52%. We observed that 62% of tRNA synthetases contain one or more domains from amongst the following four PROSITE domains: PS50862, PS00178, PS50860 and PS50861. An SVM-based model was developed to discriminate between aaRSs, and non-aaRSs, and achieved a maximum MCC of 0.68 with accuracy of 83.73%, using selective dipeptide composition. We developed a hybrid approach and achieved a maximum MCC of 0.72 with accuracy of 85.49%, where SVM model developed using selected dipeptide composition and information of four PROSITE domains. We further developed an SVM-based model for classifying the aaRSs into class-1 and class-2, using selective dipeptide composition and achieved an MCC of 0.79. We also observed that two domains (PS00178, PS50889) in class-1 and three domains (PS50862, PS50860, PS50861) in class-2 were preferred. A hybrid method was developed using these domains as descriptor, along with selected dipeptide composition, and achieved an MCC of 0.87 with a sensitivity of 94.55% and an accuracy of 93.19%. All models were evaluated using a five-fold cross-validation technique. Conclusions We have analyzed protein sequences of aaRSs (class-1 and class-2) and non-aaRSs and identified interesting patterns. The high accuracy achieved by our SVM models using selected dipeptide composition demonstrates that certain types of dipeptide are preferred in aaRSs. We were able to identify PROSITE domains that are preferred in aaRSs and their classes, providing interesting insights into tRNA synthetases. The method developed in this study will be useful for researchers studying aaRS enzymes and tRNA biology. The web-server based on the above study, is available at http://www.imtech.res.in/raghava/icaars/.
- Published
- 2010
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35. Cadmium uptake by cowpea and mungbean as affected by Cd and P application
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Singh, J. P., Panwar, B. S., and Laura, R. D.
- Subjects
PLANT physiology ,SPECTROPHOTOMETRY ,SOIL chemistry ,CROP yields ,CADMIUM ,BIOACCUMULATION ,PHOSPHORUS ,STATISTICS - Abstract
A green house experiment was conducted to determine the interactive effects of cadmium (0, 2.5, 5, 10, and 20 mg Cd kg
-1 soil)and phosphorus (0, 20 and 40 mg P kg-1 soil) on dry matter yield of cowpea and mungbean, and tissue concentration and uptake of cadmium (Cd) and Phosphorus (P). Application of Cd to soil decreased the dry matter yield of both the crops significantly at each level of applied P. Phosphorus application, on the other hand, increased the dry matter yield of both crops significantly at each level of Cd additions to the soil. Cadmium concentration in plant tissue and uptakeof Cd by plants increased markedly with the increasing rates of Cd in the soil. The magnitude of increase in tissue Cd concentration, however, was higher in the absence than in the presence of added P. Consequently, the concentration of Cd in plants decreased with increasinglevels of P application to the soil. This decrease in tissue Cd concentration with increasing P supply in the soil was mainly attributed to increased dry matter yield of crops. The concentration of P in cowpea and mungbean tissue increased while the uptake of P by these crops decreased markedly with increasing levels of Cd in the soil, irrespective of the rates of P applied. [ABSTRACT FROM AUTHOR]- Published
- 1999
36. Safety and tolerability of AMG 330 in adults with relapsed/refractory AML: a phase 1a dose-escalation study.
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Ravandi F, Subklewe M, Walter RB, Vachhani P, Ossenkoppele G, Buecklein V, Döhner H, Jongen-Lavrencic M, Baldus CD, Fransecky L, Pardee TS, Kantarjian H, Yen PK, Mukundan L, Panwar B, Yago MR, Agarwal S, Khaldoyanidi SK, and Stein A
- Subjects
- Humans, Male, Female, Middle Aged, Adult, Aged, Treatment Outcome, Young Adult, Maximum Tolerated Dose, Drug Resistance, Neoplasm drug effects, Sialic Acid Binding Ig-like Lectin 3 metabolism, Recurrence, Aged, 80 and over, Neoplasm Recurrence, Local drug therapy, Neoplasm Recurrence, Local pathology, Dose-Response Relationship, Drug, Cytokine Release Syndrome etiology, Leukemia, Myeloid, Acute drug therapy, Leukemia, Myeloid, Acute pathology, Leukemia, Myeloid, Acute diagnosis, Antibodies, Bispecific administration & dosage, Antibodies, Bispecific adverse effects, Antibodies, Bispecific therapeutic use
- Abstract
AMG 330, a bispecific T-cell engager (BiTE®) that binds CD33 and CD3 on T cells facilitates T-cell-mediated cytotoxicity against CD33+ cells. This first-in-human, open-label, dose-escalation study evaluated the safety, pharmacokinetics, pharmacodynamics, and preliminary efficacy of AMG 330 in adults with relapsed/refractory acute myeloid leukemia (R/R AML). Amongst 77 patients treated with AMG 330 (0.5 µg/day-1.6 mg/day) on 14-day or 28-day cycles, maximum tolerated dose was not reached; median duration of treatment was 29 days. The most frequent treatment-related adverse events were cytokine release syndrome (CRS; 78%) and rash (30%); 10% of patients experienced grade 3/4 CRS. CRS was mitigated with stepwise dosing of AMG 330, prophylactic dexamethasone, and early treatment with tocilizumab. Among 60 evaluable patients, eight achieved complete remission or morphologic leukemia-free state; of the 52 non-responders, 37% had ≥50% reduction in AML bone marrow blasts. AMG 330 is a promising CD33-targeted therapeutic strategy for R/R AML.
- Published
- 2024
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37. Single agent subcutaneous blinatumomab for advanced acute lymphoblastic leukemia.
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Jabbour E, Zugmaier G, Agrawal V, Martínez-Sánchez P, Rifón Roca JJ, Cassaday RD, Böll B, Rijneveld A, Abdul-Hay M, Huguet F, Cluzeau T, Díaz MT, Vucinic V, González-Campos J, Rambaldi A, Schwartz S, Berthon C, Hernández-Rivas JM, Gordon PR, Brüggemann M, Hamidi A, Chen Y, Wong HL, Panwar B, Katlinskaya Y, Markovic A, and Kantarjian H
- Subjects
- Adult, Humans, Remission Induction, Pathologic Complete Response, Acute Disease, Neoplasm, Residual, Precursor Cell Lymphoblastic Leukemia-Lymphoma drug therapy, Antibodies, Bispecific adverse effects, Lymphoma, B-Cell drug therapy, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma drug therapy, Antineoplastic Agents adverse effects
- Abstract
Blinatumomab is a BiTE® (bispecific T-cell engager) molecule that redirects CD3
+ T-cells to engage and lyse CD19+ target cells. Here we demonstrate that subcutaneous (SC) blinatumomab can provide high efficacy and greater convenience of administration. In the expansion phase of a multi-institutional phase 1b trial (ClinicalTrials.gov, NCT04521231), heavily pretreated adults with relapsed/refractory B-cell acute lymphoblastic leukemia (R/R B-ALL) received SC blinatumomab at two doses: (1) 250 μg once daily (QD) for week 1 and 500 μg three times weekly (TIW) thereafter (250 μg/500 μg) or (2) 500 μg QD for week 1 and 1000 μg TIW thereafter (500 μg/1000 μg). The primary endpoint was complete remission/complete remission with partial hematologic recovery (CR/CRh) within two cycles. At the data cutoff of September 15, 2023, 29 patients were treated: 14 at the 250 μg/500 μg dose and 13 at 500 μg/1000 μg dose. Data from two ineligible patients were excluded. At the end of two cycles, 12 of 14 patients (85.7%) from the 250 μg/500 μg dose achieved CR/CRh of which nine patients (75.0%) were negative for measurable residual disease (MRD; <10-4 leukemic blasts). At the 500 μg/1000 μg dose, 12 of 13 patients (92.3%) achieved CR/CRh; all 12 patients (100.0%) were MRD-negative. No treatment-related grade 4 cytokine release syndrome (CRS) or neurologic events (NEs) were reported. SC injections were well tolerated and all treatment-related grade 3 CRS and NEs responded to standard-of-care management, interruption, or discontinuation. Treatment with SC blinatumomab resulted in high efficacy, with high MRD-negativity rates and acceptable safety profile in heavily pretreated adults with R/R B-ALL., (© 2024 The Authors. American Journal of Hematology published by Wiley Periodicals LLC.)- Published
- 2024
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38. Intratumoral follicular regulatory T cells curtail anti-PD-1 treatment efficacy.
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Eschweiler S, Clarke J, Ramírez-Suástegui C, Panwar B, Madrigal A, Chee SJ, Karydis I, Woo E, Alzetani A, Elsheikh S, Hanley CJ, Thomas GJ, Friedmann PS, Sanchez-Elsner T, Ay F, Ottensmeier CH, and Vijayanand P
- Subjects
- Animals, Antineoplastic Agents therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, CD8-Positive T-Lymphocytes immunology, Cell Line, Tumor, Disease Models, Animal, Female, Humans, Mice, Mice, Inbred C57BL, T Follicular Helper Cells immunology, Tumor Microenvironment immunology, CTLA-4 Antigen antagonists & inhibitors, Immune Checkpoint Inhibitors therapeutic use, Lymphocytes, Tumor-Infiltrating immunology, Melanoma drug therapy, Programmed Cell Death 1 Receptor antagonists & inhibitors, T-Lymphocytes, Regulatory immunology
- Abstract
Immune-checkpoint blockade (ICB) has shown remarkable clinical success in boosting antitumor immunity. However, the breadth of its cellular targets and specific mode of action remain elusive. We find that tumor-infiltrating follicular regulatory T (T
FR ) cells are prevalent in tumor tissues of several cancer types. They are primarily located within tertiary lymphoid structures and exhibit superior suppressive capacity and in vivo persistence as compared with regulatory T cells, with which they share a clonal and developmental relationship. In syngeneic tumor models, anti-PD-1 treatment increases the number of tumor-infiltrating TFR cells. Both TFR cell deficiency and the depletion of TFR cells with anti-CTLA-4 before anti-PD-1 treatment improve tumor control in mice. Notably, in a cohort of 271 patients with melanoma, treatment with anti-CTLA-4 followed by anti-PD-1 at progression was associated with better a survival outcome than monotherapy with anti-PD-1 or anti-CTLA-4, anti-PD-1 followed by anti-CTLA-4 at progression or concomitant combination therapy., (© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.)- Published
- 2021
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39. Multi-cell type gene coexpression network analysis reveals coordinated interferon response and cross-cell type correlations in systemic lupus erythematosus.
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Panwar B, Schmiedel BJ, Liang S, White B, Rodriguez E, Kalunian K, McKnight AJ, Soloff R, Seumois G, Vijayanand P, and Ay F
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- B-Lymphocytes immunology, B-Lymphocytes metabolism, Humans, Monocytes immunology, Monocytes metabolism, T-Lymphocytes, Helper-Inducer immunology, T-Lymphocytes, Helper-Inducer metabolism, Gene Regulatory Networks, Interferons genetics, Interferons immunology, Lupus Erythematosus, Systemic drug therapy, Lupus Erythematosus, Systemic genetics, Lupus Erythematosus, Systemic immunology
- Abstract
Systemic lupus erythematosus (SLE) is an incurable autoimmune disease disproportionately affecting women. A major obstacle in finding targeted therapies for SLE is its remarkable heterogeneity in clinical manifestations as well as in the involvement of distinct cell types. To identify cell-specific targets as well as cross-correlation relationships among expression programs of different cell types, we here analyze six major circulating immune cell types from SLE patient blood. Our results show that presence of an interferon response signature stratifies patients into two distinct groups (IFNneg vs. IFNpos). Comparing these two groups using differential gene expression and differential gene coexpression analysis, we prioritize a relatively small list of genes from classical monocytes including two known immune modulators: TNFSF13B/BAFF (target of belimumab , an approved therapeutic for SLE) and IL1RN (the basis of anakinra, a therapeutic for rheumatoid arthritis). We then develop a multi-cell type extension of the weighted gene coexpression network analysis (WGCNA) framework, termed mWGCNA. Applying mWGCNA to RNA-seq data from six sorted immune cell populations (15 SLE, 10 healthy donors), we identify a coexpression module with interferon-stimulated genes (ISGs) among all cell types and a cross-cell type correlation linking expression of specific T helper cell markers to B cell response as well as to TNFSF13B expression from myeloid cells, all of which in turn correlates with disease severity of IFNpos patients. Our results demonstrate the power of a hypothesis-free and data-driven approach to discover drug targets and to reveal novel cross-correlation across cell types in SLE with implications for other autoimmune diseases., (© 2021 Panwar et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2021
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40. Effect of Ferric Citrate versus Ferrous Sulfate on Iron and Phosphate Parameters in Patients with Iron Deficiency and CKD: A Randomized Trial.
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Womack R, Berru F, Panwar B, and Gutiérrez OM
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- Aged, Alabama, Anemia, Iron-Deficiency blood, Anemia, Iron-Deficiency diagnosis, Anemia, Iron-Deficiency etiology, Biomarkers blood, Female, Ferritins blood, Humans, Iron blood, Male, Middle Aged, Renal Insufficiency, Chronic diagnosis, Time Factors, Treatment Outcome, Anemia, Iron-Deficiency drug therapy, Ferric Compounds therapeutic use, Ferrous Compounds therapeutic use, Hematinics therapeutic use, Iron Deficiencies, Phosphates blood, Renal Insufficiency, Chronic complications
- Abstract
Background and Objectives: Ferric citrate is an oral medication approved for treatment of iron deficiency anemia in patients with CKD not requiring dialysis. The relative efficacy of ferric citrate versus ferrous sulfate in treating iron deficiency in patients with CKD is unclear., Design, Setting, Participants, & Measurements: We randomized 60 adults with moderate to severe CKD (eGFR 15-45 ml/min per 1.73 m
2 ) and iron deficiency (transferrin saturation [TSAT] ≤30% and ferritin ≤300 ng/ml) to ferric citrate (2 g three times a day with meals, n =30) or ferrous sulfate (325 mg three times a day, n =30) for 12 weeks. Primary outcomes were change in TSAT and ferritin from baseline to 12 weeks. Secondary outcomes were change in hemoglobin, fibroblast growth factor 23 (FGF23), and hepcidin., Results: Baseline characteristics were well balanced between study arms. There was a greater increase in TSAT (between-group difference in mean change, 8%; 95% confidence interval [95% CI], 1 to 15; P =0.02) and ferritin (between-group difference in mean change, 37 ng/ml; 95% CI, 10 to 64; P =0.009) from baseline to 12 weeks in participants randomized to ferric citrate as compared with ferrous sulfate. Similarly, as compared with ferrous sulfate, treatment with ferric citrate resulted in a greater increase in hepcidin from baseline to 12 weeks (between-group difference, 69 pg/ml; 95% CI, 8 to 130). There were no between-group differences in mean change for hemoglobin (0.3 g/dl; 95% CI, -0.2 to 0.8), intact FGF23 (-29 pg/ml; 95% CI, -59 to 0.1), or C-terminal FGF23 (61 RU/ml; 95% CI, -181 to 58). The incidence of adverse events did not differ between treatment arms., Conclusions: As compared with ferrous sulfate, treatment with ferric citrate for 12 weeks resulted in a greater mean increase in TSAT and ferritin concentrations in individuals with moderate to severe CKD and iron deficiency., Clinical Trial Registry Name and Registration Number: Impact of Ferric Citrate vs Ferrous Sulfate on Iron Parameters and Hemoglobin in Individuals With Moderate to Severe Chronic Kidney Disease (CKD) With Iron Deficiency, NCT02888171., (Copyright © 2020 by the American Society of Nephrology.)- Published
- 2020
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41. Bioinformatics drives discovery in Biomedicine.
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Gujar R, Panwar B, and Dhanda SK
- Abstract
Bioinformatics has evolved from providing basic solutions, such as sequence alignment, structure predictions, and phylogenetic analysis to an independent data-driven field. The unprecedented growth of genomic technologies and the enormous data have opened an avenue for bioinformaticians (Bioinformatics professionals) never been seen before in the history of mankind. The novel opportunity also requires creative solutions that need skills to deal with noisy, unstructured information to offer valuable biological insights. Currently, we are seeing only the tip of an iceberg and the future will revolve around big data sets in all forms of biological research. The emerging challenge is to unfold the hidden iceberg of data., (© 2020 Biomedical Informatics.)
- Published
- 2020
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42. Single-cell transcriptomic analysis of tissue-resident memory T cells in human lung cancer.
- Author
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Clarke J, Panwar B, Madrigal A, Singh D, Gujar R, Wood O, Chee SJ, Eschweiler S, King EV, Awad AS, Hanley CJ, McCann KJ, Bhattacharyya S, Woo E, Alzetani A, Seumois G, Thomas GJ, Ganesan AP, Friedmann PS, Sanchez-Elsner T, Ay F, Ottensmeier CH, and Vijayanand P
- Subjects
- Cell Proliferation, Clone Cells, Cytotoxicity, Immunologic genetics, Hepatitis A Virus Cellular Receptor 2 metabolism, Humans, Lung metabolism, Lung pathology, Lymphocyte Subsets immunology, Lymphocytes, Tumor-Infiltrating immunology, Programmed Cell Death 1 Receptor metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Transcription, Genetic, Gene Expression Profiling, Immunologic Memory genetics, Lung Neoplasms genetics, Lung Neoplasms immunology, Single-Cell Analysis, T-Lymphocytes immunology, Transcriptome genetics
- Abstract
High numbers of tissue-resident memory T (T
RM ) cells are associated with better clinical outcomes in cancer patients. However, the molecular characteristics that drive their efficient immune response to tumors are poorly understood. Here, single-cell and bulk transcriptomic analysis of TRM and non-TRM cells present in tumor and normal lung tissue from patients with lung cancer revealed that PD-1-expressing TRM cells in tumors were clonally expanded and enriched for transcripts linked to cell proliferation and cytotoxicity when compared with PD-1-expressing non-TRM cells. This feature was more prominent in the TRM cell subset coexpressing PD-1 and TIM-3, and it was validated by functional assays ex vivo and also reflected in their chromatin accessibility profile. This PD-1+ TIM-3+ TRM cell subset was enriched in responders to PD-1 inhibitors and in tumors with a greater magnitude of CTL responses. These data highlight that not all CTLs expressing PD-1 are dysfunctional; on the contrary, TRM cells with PD-1 expression were enriched for features suggestive of superior functionality., (© 2019 Clarke et al.)- Published
- 2019
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43. Human Eosinophils Express a Distinct Gene Expression Program in Response to IL-3 Compared with Common β-Chain Cytokines IL-5 and GM-CSF.
- Author
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Nelson RK, Brickner H, Panwar B, Ramírez-Suástegui C, Herrera-de la Mata S, Liu N, Diaz D, Alexander LEC, Ay F, Vijayanand P, Seumois G, and Akuthota P
- Subjects
- Asthma immunology, Down-Regulation immunology, Humans, Signal Transduction immunology, Up-Regulation immunology, Cytokines immunology, Eosinophils immunology, Gene Expression immunology, Granulocyte-Macrophage Colony-Stimulating Factor immunology, Interleukin-3 immunology, Interleukin-5 immunology
- Abstract
Despite recent advances in asthma management with anti-IL-5 therapies, many patients have eosinophilic asthma that remains poorly controlled. IL-3 shares a common β subunit receptor with both IL-5 and GM-CSF but, through α-subunit-specific properties, uniquely influences eosinophil biology and may serve as a potential therapeutic target. We aimed to globally characterize the transcriptomic profiles of GM-CSF, IL-3, and IL-5 stimulation on human circulating eosinophils and identify differences in gene expression using advanced statistical modeling. Human eosinophils were isolated from the peripheral blood of healthy volunteers and stimulated with either GM-CSF, IL-3, or IL-5 for 48 h. RNA was then extracted and bulk sequencing performed. DESeq analysis identified differentially expressed genes and weighted gene coexpression network analysis independently defined modules of genes that are highly coexpressed. GM-CSF, IL-3, and IL-5 commonly upregulated 252 genes and downregulated 553 genes, producing a proinflammatory and survival phenotype that was predominantly mediated through TWEAK signaling. IL-3 stimulation yielded the most numbers of differentially expressed genes that were also highly coexpressed ( n = 119). These genes were enriched in pathways involving JAK/STAT signaling. GM-CSF and IL-5 stimulation demonstrated redundancy in eosinophil gene expression. In conclusion, IL-3 produces a distinct eosinophil gene expression program among the β-chain receptor cytokines. IL-3-upregulated genes may provide a foundation for research into therapeutics for patients with eosinophilic asthma who do not respond to anti-IL-5 therapies., (Copyright © 2019 by The American Association of Immunologists, Inc.)
- Published
- 2019
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44. Plasma 25-Hydroxyvitamin D and the Longitudinal Risk of Sepsis in the REGARDS Cohort.
- Author
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Kempker JA, Panwar B, Judd SE, Jenny NS, Wang HE, and Gutiérrez OM
- Subjects
- Aged, Aged, 80 and over, Community-Acquired Infections etiology, Female, Hospitalization, Humans, Longitudinal Studies, Male, Middle Aged, Proportional Hazards Models, Public Health, Risk Factors, Sepsis prevention & control, United States, Vitamin D blood, Community-Acquired Infections complications, Population Health statistics & numerical data, Sepsis etiology, Vitamin D analogs & derivatives
- Abstract
Background: Low baseline plasma 25-hydroxyvitamin D (25(OH)D) is associated with increased risk of acute respiratory infections, but its association with long-term risk of sepsis remains unclear., Methods: We performed a case-cohort analysis of participants selected from the Reasons for Geographic and Racial Differences in Stroke (REGARDS) study, a US cohort of 30239 adults aged ≥45 years. We measured baseline plasma 25(OH)D in 711 sepsis cases and in 992 participants randomly selected from the REGARDS cohort. We captured sepsis events by screening records with International Classification of Disease methods and then adjudicating clinical charts for significant, suspected infection and severe inflammatory response syndrome criteria on presentation., Results: In the study sample, the median age of participants was 65.0 years, 41% self-identified as black, and 45% were male. Mean plasma 25(OH)D concentration was 25.8 ng/mL; for 31% of participants, it was <20 ng/mL. The adjusted risk of community-acquired sepsis was higher for each lower category of baseline 25(OH)D. Specifically, in a Cox proportional hazards model adjusting for multiple potential confounders, when compared to a baseline 25(OH)D >33.6 ng/mL, lower 25(OH)D groups were associated with higher hazards of sepsis (16.5-22.4 ng/mL; hazard ratio [HR]; 3.21; 95% confidence interval [CI], 1.98 to 5.21 and <16.5 ng/mL; HR, 6.81, 95% CI, 3.95 to 11.73). Results did not materially differ in analyses stratified by race or age., Conclusions: In the REGARDS cohort of community-dwelling US adults, low plasma 25(OH)D measured at a time of relative health was independently associated with increased risk of sepsis., (© The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
- Published
- 2019
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45. Reduced expression of phosphatase PTPN2 promotes pathogenic conversion of Tregs in autoimmunity.
- Author
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Svensson MN, Doody KM, Schmiedel BJ, Bhattacharyya S, Panwar B, Wiede F, Yang S, Santelli E, Wu DJ, Sacchetti C, Gujar R, Seumois G, Kiosses WB, Aubry I, Kim G, Mydel P, Sakaguchi S, Kronenberg M, Tiganis T, Tremblay ML, Ay F, Vijayanand P, and Bottini N
- Subjects
- Animals, Arthritis, Rheumatoid genetics, Arthritis, Rheumatoid pathology, Disease Models, Animal, Female, Forkhead Transcription Factors genetics, Forkhead Transcription Factors immunology, Interleukin-17 genetics, Interleukin-17 immunology, Interleukin-6 genetics, Interleukin-6 immunology, Mice, Mice, Inbred BALB C, Mice, Knockout, Nuclear Receptor Subfamily 1, Group F, Member 3 genetics, Nuclear Receptor Subfamily 1, Group F, Member 3 immunology, Protein Tyrosine Phosphatase, Non-Receptor Type 2 genetics, STAT3 Transcription Factor genetics, STAT3 Transcription Factor immunology, T-Lymphocytes, Regulatory pathology, Arthritis, Rheumatoid immunology, Protein Tyrosine Phosphatase, Non-Receptor Type 2 immunology, T-Lymphocytes, Regulatory immunology
- Abstract
Genetic variants at the PTPN2 locus, which encodes the tyrosine phosphatase PTPN2, cause reduced gene expression and are linked to rheumatoid arthritis (RA) and other autoimmune diseases. PTPN2 inhibits signaling through the T cell and cytokine receptors, and loss of PTPN2 promotes T cell expansion and CD4- and CD8-driven autoimmunity. However, it remains unknown whether loss of PTPN2 in FoxP3+ regulatory T cells (Tregs) plays a role in autoimmunity. Here we aimed to model human autoimmune-predisposing PTPN2 variants, the presence of which results in a partial loss of PTPN2 expression, in mouse models of RA. We identified that reduced expression of Ptpn2 enhanced the severity of autoimmune arthritis in the T cell-dependent SKG mouse model and demonstrated that this phenotype was mediated through a Treg-intrinsic mechanism. Mechanistically, we found that through dephosphorylation of STAT3, PTPN2 inhibits IL-6-driven pathogenic loss of FoxP3 after Tregs have acquired RORγt expression, at a stage when chromatin accessibility for STAT3-targeted IL-17-associated transcription factors is maximized. We conclude that PTPN2 promotes FoxP3 stability in mouse RORγt+ Tregs and that loss of function of PTPN2 in Tregs contributes to the association between PTPN2 and autoimmunity.
- Published
- 2019
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46. Association of Fibroblast Growth Factor 23 With Risk of Incident Coronary Heart Disease in Community-Living Adults.
- Author
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Panwar B, Judd SE, Wadley VG, Jenny NS, Howard VJ, Safford MM, and Gutiérrez OM
- Subjects
- Coronary Disease etiology, Female, Fibroblast Growth Factor-23, Humans, Independent Living statistics & numerical data, Male, Middle Aged, Racial Groups statistics & numerical data, Renal Insufficiency, Chronic blood, Risk Factors, Sex Factors, Coronary Disease blood, Fibroblast Growth Factors blood
- Abstract
Importance: Higher circulating fibroblast growth factor 23 (FGF23) concentrations are associated with cardiovascular disease events linked to heart failure, but associations of FGF23 with coronary heart disease (CHD) have been less consistent., Objective: To determine the association of plasma FGF23 concentrations with incident CHD and whether this association differs by race, sex, or chronic kidney disease status., Design, Setting, and Participants: We examined the association of FGF23 concentrations with incident CHD risk within the Reasons for Geographic and Racial Differences in Stroke study, a prospective cohort of black and white adults 45 years and older enrolled between January 2003 and October 2007 with follow-up through December 31, 2011. Using a case-cohort design, we measured FGF23 concentrations in 829 participants who developed incident CHD and in 812 participants randomly selected from the Reasons for Geographic and Racial Differences in Stroke study cohort (cohort random sample). To account for the stratified sampling design, the cohort random sample was weighted back to the original cohort overall (n = 22 127). Cox proportional hazards models were used to examine the association of FGF23 concentration with incident CHD, adjusting for CHD risk factors and kidney function. In prespecified analyses, we examined whether race, sex, or chronic kidney disease modified the association of FGF23 concentration with incident CHD., Exposures: Plasma C-terminal FGF23 concentrations., Main Outcomes and Measures: Investigator-adjudicated incident CHD events., Results: Of the 22 127 participants in the weighted cohort random sample, 13 059 (58.9%) were female and 9435 (42.6%) were black, and the mean age was 64.3 (95% CI, 63.7-64.9) years. Greater age, lower estimated glomerular filtration rate, higher urine albumin to creatinine ratio, and female sex were associated with higher FGF23 concentration at baseline. In multivariable models adjusted for established CHD risk factors and kidney function, higher FGF23 concentrations were associated with greater risk of CHD (hazard ratio [HR] comparing fourth with first quartile, 2.15; 95% CI, 1.35-3.42). The magnitude and strength of these associations differed by sex. However, these differences were no longer observed when adjusting for hormone therapy in women (men: HR comparing fourth with first quartile, 2.40; 95% CI, 1.30-4.42; women: HR comparing fourth with first quartile, 2.34; 95% CI, 1.04-5.27) or when using sex-specific FGF23 quartiles (men: HR comparing fourth with first quartile, 2.65; 95% CI, 1.43-4.90; women: HR comparing fourth with first quartile, 2.26; 95% CI, 1.02-5.03)., Conclusions and Relevance: Higher FGF23 concentrations were associated with greater risk of CHD. Heterogeneity in the association by sex may be caused by differences in the distribution of plasma FGF23 concentrations or the use of hormone therapy in men vs women.
- Published
- 2018
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47. Effect of calcitriol on serum hepcidin in individuals with chronic kidney disease: a randomized controlled trial.
- Author
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Panwar B, McCann D, Olbina G, Westerman M, and Gutiérrez OM
- Subjects
- Aged, Biomarkers blood, Calcium Channel Agonists therapeutic use, Double-Blind Method, Female, Humans, Male, Middle Aged, Treatment Outcome, Calcitriol therapeutic use, Hepcidins blood, Renal Insufficiency, Chronic blood, Renal Insufficiency, Chronic drug therapy
- Abstract
Background: Anemia is highly prevalent in chronic kidney disease (CKD). Elevated hepcidin concentrations are an important mediator of disordered iron metabolism, a key mechanism underlying anemia of CKD. Vitamin D was recently shown to reduce serum hepcidin concentrations in healthy individuals. We examined whether treatment with calcitriol reduces serum hepcidin in individuals with CKD., Methods: A total of 40 participants with stage 3 or 4 CKD (eGFR 15-60 ml/min/1.73m
2 ) were randomized to receive either oral calcitriol 0.5 mcg daily or identically-matched placebo for 6 weeks. The primary outcome variable was change in serum hepcidin concentrations. Secondary outcomes variables included the change in iron parameters, calcium, phosphorus, intact parathyroid hormone and hemoglobin concentrations. Study samples were drawn at baseline, 3 days, 1 week, 4 weeks and 6 weeks after randomization. Repeated measures analysis was used to examine differences in outcome variables over time in the two groups., Results: There were no significant differences in the baseline characteristics between the placebo and calcitriol arms. Over 6 weeks of follow-up there were no significant differences in the change in serum hepcidin, iron parameters, or hemoglobin between the two groups. Serum calcium and phosphorus significantly increased and PTH significantly decreased after 6 weeks in calcitriol group whereas these analytes did not change in the placebo group., Conclusion: Calcitriol did not reduce serum hepcidin concentrations among individuals with mild to moderate CKD. Future studies are needed to assess if nutritional forms of vitamin D affect hepcidin concentrations in CKD., Trial Registration: ClinicalTrials.gov Identifier: NCT01988116 . Registered: November 4, 2013.- Published
- 2018
- Full Text
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48. Accurate prediction of personalized olfactory perception from large-scale chemoinformatic features.
- Author
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Li H, Panwar B, Omenn GS, and Guan Y
- Subjects
- Humans, Machine Learning, Molecular Structure, Structure-Activity Relationship, Computational Biology methods, Decision Trees, Odorants analysis, Olfactory Perception physiology, Smell physiology
- Abstract
Background: The olfactory stimulus-percept problem has been studied for more than a century, yet it is still hard to precisely predict the odor given the large-scale chemoinformatic features of an odorant molecule. A major challenge is that the perceived qualities vary greatly among individuals due to different genetic and cultural backgrounds. Moreover, the combinatorial interactions between multiple odorant receptors and diverse molecules significantly complicate the olfaction prediction. Many attempts have been made to establish structure-odor relationships for intensity and pleasantness, but no models are available to predict the personalized multi-odor attributes of molecules. In this study, we describe our winning algorithm for predicting individual and population perceptual responses to various odorants in the DREAM Olfaction Prediction Challenge., Results: We find that random forest model consisting of multiple decision trees is well suited to this prediction problem, given the large feature spaces and high variability of perceptual ratings among individuals. Integrating both population and individual perceptions into our model effectively reduces the influence of noise and outliers. By analyzing the importance of each chemical feature, we find that a small set of low- and nondegenerative features is sufficient for accurate prediction., Conclusions: Our random forest model successfully predicts personalized odor attributes of structurally diverse molecules. This model together with the top discriminative features has the potential to extend our understanding of olfactory perception mechanisms and provide an alternative for rational odorant design.
- Published
- 2018
- Full Text
- View/download PDF
49. Comparison of spinocerebellar ataxia type 3 mouse models identifies early gain-of-function, cell-autonomous transcriptional changes in oligodendrocytes.
- Author
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Ramani B, Panwar B, Moore LR, Wang B, Huang R, Guan Y, and Paulson HL
- Subjects
- Animals, Ataxin-3 metabolism, B-Cell Activation Factor Receptor metabolism, Brain metabolism, Brain Stem, Disease Models, Animal, Exons, Humans, Machado-Joseph Disease genetics, Machado-Joseph Disease metabolism, Mice, Nerve Tissue Proteins genetics, Nuclear Proteins genetics, Oligodendroglia metabolism, Peptides metabolism, Repressor Proteins metabolism, Spinocerebellar Ataxias metabolism, Trinucleotide Repeats, Ataxin-3 genetics, Spinocerebellar Ataxias genetics
- Abstract
Spinocerebellar ataxia type 3 (SCA3) is a neurodegenerative disorder caused by a polyglutamine-encoding CAG repeat expansion in the ATXN3 gene. This expansion leads to misfolding and aggregation of mutant ataxin-3 (ATXN3) and degeneration of select brain regions. A key unanswered question in SCA3 and other polyglutamine diseases is the extent to which neurodegeneration is mediated through gain-of-function versus loss-of-function. To address this question in SCA3, we performed transcriptional profiling on the brainstem, a highly vulnerable brain region in SCA3, in a series of mouse models with varying degrees of ATXN3 expression and aggregation. We include two SCA3 knock-in mouse models: our previously published model that erroneously harbors a tandem duplicate of the CAG repeat-containing exon, and a corrected model, introduced here. Both models exhibit dose-dependent neuronal accumulation and aggregation of mutant ATXN3, but do not exhibit a behavioral phenotype. We identified a molecular signature that correlates with ATXN3 neuronal aggregation yet is primarily linked to oligodendrocytes, highlighting early white matter dysfunction in SCA3. Two robustly elevated oligodendrocyte transcripts, Acy3 and Tnfrsf13c, were confirmed as elevated at the protein level in SCA3 human disease brainstem. To determine if mutant ATXN3 acts on oligodendrocytes cell-autonomously, we manipulated the repeat expansion in the variant SCA3 knock-in mouse by cell-type specific Cre/LoxP recombination. Changes in oligodendrocyte transcripts are driven cell-autonomously and occur independent of neuronal ATXN3 aggregation. Our findings support a primary toxic gain of function mechanism and highlight a previously unrecognized role for oligodendrocyte dysfunction in SCA3 disease pathogenesis., (© The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
- Published
- 2017
- Full Text
- View/download PDF
50. GATA3 Abundance Is a Critical Determinant of T Cell Receptor β Allelic Exclusion.
- Author
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Ku CJ, Sekiguchi JM, Panwar B, Guan Y, Takahashi S, Yoh K, Maillard I, Hosoya T, and Engel JD
- Subjects
- Animals, GATA3 Transcription Factor genetics, Gene Expression Regulation, Gene Ontology, Mice, Inbred C57BL, Mice, Transgenic, Models, Biological, Mutation genetics, Protein Binding, RNA, Messenger genetics, RNA, Messenger metabolism, Sequence Analysis, RNA, Spleen metabolism, Thymocytes metabolism, V(D)J Recombination genetics, Alleles, GATA3 Transcription Factor metabolism, Receptors, Antigen, T-Cell, alpha-beta genetics
- Abstract
Allelic exclusion describes the essential immunological process by which feedback repression of sequential DNA rearrangements ensures that only one autosome expresses a functional T or B cell receptor. In wild-type mammals, approximately 60% of cells have recombined the DNA of one T cell receptor β (TCRβ) V-to-DJ-joined allele in a functional configuration, while the second allele has recombined only the DJ sequences; the other 40% of cells have recombined the V to the DJ segments on both alleles, with only one of the two alleles predicting a functional TCRβ protein. Here we report that the transgenic overexpression of GATA3 leads predominantly to biallelic TCRβ gene ( Tcrb ) recombination. We also found that wild-type immature thymocytes can be separated into distinct populations based on intracellular GATA3 expression and that GATA3
LO cells had almost exclusively recombined only one Tcrb locus (that predicted a functional receptor sequence), while GATA3HI cells had uniformly recombined both Tcrb alleles (one predicting a functional and the other predicting a nonfunctional rearrangement). These data show that GATA3 abundance regulates the recombination propensity at the Tcrb locus and provide new mechanistic insight into the historic immunological conundrum for how Tcrb allelic exclusion is mediated., (Copyright © 2017 American Society for Microbiology.)- Published
- 2017
- Full Text
- View/download PDF
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