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157 results on '"Pandhare, Jui"'

8. HIV-1 Preintegration Complex Preferentially Integrates the Viral DNA into Nucleosomes Containing Trimethylated Histone 3-Lysine 36 Modification and Flanking Linker DNA

Author

Sapp, Nicklas, primary, Burge, Nathaniel, additional, Cox, Khan, additional, Prakash, Prem, additional, Balasubramaniam, Muthukumar, additional, Thapa, Santosh, additional, Christensen, Devin, additional, Li, Min, additional, Linderberger, Jared, additional, Kvaratskhelia, Mamuka, additional, Pandhare, Jui, additional, Craigie, Robert, additional, Poirier, Michael G., additional, and Dash, Chandravanu, additional

Published
2022
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11. HIV-1 preintegration complex preferentially integrates the viral DNA into nucleosomes containing trimethylated histone 3-lysine 36 modification

Author

Sapp, Nicklas E, primary, Burge, Nathaniel, additional, Cox, Khan, additional, Thapa, Santosh, additional, Prakash, Prem, additional, Balasubramaniam, Muthukumar, additional, Christensen, Devin, additional, Li, Min, additional, Linderberger, Jared, additional, Kvaratskhelia, Mamuka, additional, Pandhare, Jui, additional, Craigie, Robert, additional, Sundquist, Wes I., additional, Poirier, Michael G, additional, and Dash, Chandravanu, additional

Published
2022
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21. The metabolism of proline as microenvironmental stress substrate

Author

Phang, James M., Pandhare, Jui, and Liu, Yongmin

Subjects
Proline -- Physiological aspects, Proline -- Health aspects, Amino acid metabolism -- Health aspects, Food/cooking/nutrition
Abstract

Proline, a unique proteogenic secondary amino acid, has its own metabolic system with special features. Recent findings defining the regulation of this system led us to propose that proline is a stress substrate in the microenvironment of inflammation and tumorigenesis. The criteria for proline as a stress substrate are: 1) the enzymes utilizing proline respond to stress signaling; 2) there is a large, mobilizable pool of proline; and 3) the metabolism of proline serves special stress functions. Studies show that the proline-utilizing enzyme, proline oxidase (POX)/proline dehydrogenase (PRODH), responds to genotoxic, inflammatory, and nutrient stress. Proline as substrate is stored as collagen in extracellular matrix, connective tissue, and bone and it is rapidly released from this reservoir by the sequential action of matrix metalloproteinases, peptidases, and prolidase. Special functions include the use of proline by POX/PRODH to generate superoxide radicals that initiate apoptosis by intrinsic and extrinsic pathways. Under conditions of nutrient stress, proline is an energy source. It provides carbons for the tricarboxylic acid cycle and also participates in the proline cycle. The latter, catalyzed by mitochondrial POX and cytosolic pyrroline-5-carboxylate reductase, shuttles reducing potential from the pentose phosphate pathway into mitochondria to generate ATP and oxidizing potential to activate the cytosolic pentose phosphate pathway.

Published
2008

26. Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities

Author

Kaddour, Hussein, primary, Lyu, Yuan, additional, Welch, Jennifer L., additional, Paromov, Victor, additional, Mandape, Sammed N., additional, Sakhare, Shruti S., additional, Pandhare, Jui, additional, Stapleton, Jack T., additional, Pratap, Siddharth, additional, Dash, Chandravanu, additional, and Okeoma, Chioma M., additional

Published
2020
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31. Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

Author

Balasubramaniam, Muthukumar, Davids, Benem, Addai, Amma B., Pandhare, Jui, and Dash, Chandravanu

Subjects
Cell Nucleus, Cytoplasm, Nucleoproteins, Virus Integration, DNA, Viral, HIV-1, Humans, Genome, Viral, HIV Integrase, biochemical phenomena, metabolism, and nutrition, Infection
Abstract

HIV-1 envelope proteins engage cognate receptors on the target cell surface, which leads to viral-cell membrane fusion followed by the release of the viral capsid (CA) core into the cytoplasm. Subsequently, the viral Reverse Transcriptase (RT), as part of a namesake nucleoprotein complex termed the Reverse Transcription Complex (RTC), converts the viral single-stranded RNA genome into a double-stranded DNA copy (vDNA). This leads to the biogenesis of another nucleoprotein complex, termed the pre-integration complex (PIC), composed of the vDNA and associated virus proteins and host factors. The PIC-associated viral integrase (IN) orchestrates the integration of the vDNA into the host chromosomal DNA in a temporally and spatially regulated two-step process. First, the IN processes the 3' ends of the vDNA in the cytoplasm and, second, after the PIC traffics to the nucleus, it mediates integration of the processed vDNA into the chromosomal DNA. The PICs isolated from target cells acutely infected with HIV-1 are functional in vitro, as they are competent to integrate the associated vDNA into an exogenously added heterologous target DNA. Such PIC-based in vitro integration assays have significantly contributed to delineating the mechanistic details of retroviral integration and to discovering IN inhibitors. In this report, we elaborate upon an updated HIV-1 PIC assay that employs a nested real-time quantitative Polymerase Chain Reaction (qPCR)-based strategy for measuring the in vitro integration activity of isolated native PICs.

Published
2017

34. Poly (ADP-Ribose) Polymerase-1 (PARP-1) Induction by Cocaine Is Post-Transcriptionally Regulated by miR-125b

Author

Dash, Sabyasachi, primary, Balasubramaniam, Muthukumar, additional, Rana, Tanu, additional, Godino, Arthur, additional, Peck, Emily G., additional, Goodwin, Jeffery Shawn, additional, Villalta, Fernando, additional, Calipari, Erin S., additional, Nestler, Eric J., additional, Dash, Chandravanu, additional, and Pandhare, Jui, additional

Published
2017
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45. PPARγ and Proline Oxidase in Cancer

Author

Phang, James M., Pandhare, Jui, Zabirnyk, Olga, and Liu, Yongmin

Subjects
Article Subject
Abstract

Proline is metabolized by its own specialized enzymes with their own tissue and subcellular localizations and mechanisms of regulation. The central enzyme in this metabolic system is proline oxidase, a flavin adenine dinucleotide-containing enzyme which is tightly bound to mitochondrial inner membranes. The electrons from proline can be used to generate ATP or can directly reduce oxygen to form superoxide. Although proline may be derived from the diet and biosynthesized endogenously, an important source in the microenvironment is from degradation of extracellular matrix by matrix metalloproteinases. Previous studies showed that proline oxidase is a p53-induced gene and its overexpression can initiate proline-dependent apoptosis by both intrinsic and extrinsic pathways. Another important factor regulating proline oxidase is peroxisome proliferator activated receptor gamma (PPARγ). Importantly, in several cancer cells, proline oxidase may be an important mediator of the PPARγ-stimulated generation of ROS and induction of apoptosis. Knockdown of proline oxidase expression by antisense RNA markedly decreased these PPARγ-stimulated effects. These findings suggest an important role in the proposed antitumor effects of PPARγ. Moreover, it is possible that proline oxidase may contribute to the other metabolic effects of PPARγ.

Published
2008
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