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The aim of the research was to investigate the resistance and efficacy of the fungi Aspergillus awamori in removing heavy metals. We conducted an analysis of the fungus's tolerance to several heavy metals by measuring the dry weight and using a plate method. The fungus exhibited growth in concentrations and lowest growth was observed in a concentration of 400 ppm of Cd. The heavy metals were successfully removed with the following orders: Zn> Co, > Ag, > Hg and Cu. The optimal parameters for the elimination of 100 mg/L of heavy metals were a temperature of 28°C, a pH range of 4.0 to 5.5, a heavy metal concentration of 100 ppm, and a biomass of fungi of 1 g. [ABSTRACT FROM AUTHOR]

The investigation concluded that the water quality of the Gomti river was found to be severely polluted. The Gomti River was determined to have a poor physicochemical condition, indicating significant hazards and rendering it unsuitable for any purpose. In the present investigation, the pH value measured at Daliganj was 7.33±0.29, which slightly exceeded the optimal range. Conversely, the lowest recorded pH level (pH 5±1) was found in close proximity to the riverbank. Biological oxygen demand (BOD) concentration serves as a dependable biomarker for water pollution. The existence of Dissolved Oxygen (DO) is vital for the survival of fish and other aquatic creatures, and its levels are directly influenced by water temperature. The water at Daliganj had the highest reported level of Biochemical Oxygen Demand (BOD), measuring 21.67 mg/l. However, the lowest recorded value was observed at the Lat riverbank, measuring 14.33 mg/l. The river is extensively polluted as a result of the release of home and industrial garbage through multiple drainage systems. The rise in total hardness value was attributed to household discharges. The water in the Shaheed Smarak locality has elevated levels of Chemical Oxygen Demand (COD). The water quality declined as a result of the drainage system throughout its vicinity. The main cause of pollution is the diverse range of industrial, agricultural and household waste, leading to an increase in the amount of garbage being dumped into the river. Consequently, there is an increase in the concentration of toxic heavy metals, resulting in the pollution of river water. The river's self-purification capacity has been impeded due to the accumulation of both organic and inorganic debris, leading to a rise in bacterial growth. Hence, it is imperative to eliminate the biological burden prior to its entry into the river, with the aim of safeguarding the well-being of both humans and aquatic organisms. The Gomati River is experiencing water contamination due to the presence of pollutants. [ABSTRACT FROM AUTHOR]

Breast cancer is a complex illness with many different types. In the Western world, it is the largest cause of cancer-related death among women. With the rising prevalence of breast cancer, particularly lymph node negative breast cancer, precise prediction is required to ensure successful treatments and avoid under or overtreatment. Various prognostic indicators have been created in an attempt to provide reliable diagnosis and determine which therapy is best for a certain patient. Mitochondrial mito-stress testing was utilized to look at basic cell metabolism, such as ATP turnover, protons leak, maximum respiration, and reserve respiration capabilities. All of these measures were found to be lower in MCF-7 cells transfected with let-7b inhibitors than in control cells transfected with scramble, resulting in a significant reduction in ATP generation. [ABSTRACT FROM AUTHOR]

The people can survive day, weak and month with eat food, but without water people cannot survive for a day. This contamination of drinking water due to (E coli, Salmonella, Shigella, and cholera), viruses (Hepatitis A, Polio Virus and Rota Virus). In this study, drinking water collected from different area of Allahabad District. We performed the microbiological, biochemical, Molecular biological, Molecular biological to confirm the presence of E. coli, Salmonella and Shigella etc. type of pathogenic bacteria in different area of drinking water. Molecular based method is evaluated by the 16S r DNA gene sequencing technique to characterized the pathogenic bacteria belonging to family Enterobacteriaceae. Design and synthesis a set of oligonucleotides (Primer) capable of amplifying nearly full length (1500bp) 16S r-DNA from many bacteria like Clastridium perfringer, pseudomonas, Neissiria, Gonorrhaeae, Proteus, Escherichia, Eracinia, antibiotics such as Ampicillin, streptomycin, Gentamycin Ciprofloxacine and observed high background of resistant and virulence bacteria in Tap water. [ABSTRACT FROM AUTHOR]