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Mediterranean temporary ponds are recognized as conservation priority habitats that face anthropogenic threats and are important habitats for a number of aquatic and terrestrial animals and plants. Bats are a diverse group of animals that use ponds for drinking and feeding on emerging aquatic insects and terrestrial insects in the riparian zone. We investigated the importance of temporary ponds for bats in Greece by acoustically sampling bat community structures and activity at temporary ponds throughout the year. We sampled monthly, from 3 to 13 months in 2019–2020, at sites at the pond edge and approximately 150–300 m away from the edge, at four temporary ponds in northern and southern Greece. Our results confirm the importance of temporary ponds for bats as activity was recorded year-round and was high in all but the winter months. In general, the distance to the edge of the pond and the presence of water in the pond explained bat activity together with air temperature. Importantly, whether dry or not, all ponds supported bat activity, independent of their particular characteristics. This study highlights the urgent need for the conservation of temporary ponds, especially in areas with limited water availability.

In the present study we investigated the diet of Eleonora’s falcons in Greece and assessed the regional dietary pattern of 16 breeding colonies of the Aegean. Overall 224 nests were visited and a t...

Background The Schreiber’s bat, Miniopterus schreibersii, is adapted to long-distance flight, yet long distance movements have only been recorded sporadically using capture-mark-recapture. In this study, we used the hydrogen isotopic composition of 208 wing and 335 fur specimens from across the species' European range to test the hypothesis that the species migrates over long distances. Results After obtaining the hydrogen isotopic composition (δ2H) of each sample, we performed geographic assignment tests by comparing the δ2H of samples with the δ2H of sampling sites. We found that 95 bats out of 325 showed evidence of long-distance movement, based on the analysis of either fur or wing samples. The eastern European part of the species range (Greece, Bulgaria and Serbia) had the highest numbers of bats that had moved. The assignment tests also helped identify possible migratory routes, such as movement between the Alps and the Balkans. Conclusions This is the first continental-scale study to provide evidence of migratory behaviour of M. schreibersii throughout its European range. The work highlights the need for further investigation of this behaviour to provide appropriate conservation strategies.

Miniopterus schreibersii is a cave-dwelling bat species with a wide distribution in the western Palearctic spanning southern and central Europe, North Africa, Anatolia, the Middle East, and the Caucasus. We investigated the social structure and its effects on the genetic makeup of this species, using 10 nuclear microsatellite markers and a partial fragment of the mitochondrial cytochrome b gene. Samples were examined from the species' entire circum-Mediterranean range. Local structuring that was previously detected among populations of M. schreibersii using mitochondrial markers was not observed for microsatellite markers, indicating male-biased dispersal for the species. Some support was found for postglacial expansions in Europe, with Anatolia potentially acting as the primary refugium during the Last Glacial Maximum (LGM). However, support for this hypothesis is not as strong as that previously detected using mitochondrial DNA markers. This is likely due to the diminishing effect of male-mediated dispersal, replenishing the nuclear diversity faster than the mitochondrial diversity in regions that are relatively far from the glacial refugia.

WOS: 000375896000026, PubMed ID: 27001602, The isolation of populations in the Iberian, Italian and Balkan peninsulas during the ice ages define four main paradigms that explain much of the known distribution of intraspecific genetic diversity in Europe. In this study we investigated the phylogeography of a wide-spread bat species, the bent-winged bat, Miniopterus schreibersii around the Mediterranean basin and in the Caucasus. Environmental Niche Modeling (ENM) analysis was applied to predict both the current distribution of the species and its distribution during the last glacial maximum (LGM). The combination of genetics and ENM results suggest that the populations of M. schreibersii in Europe, the Caucasus and Anatolia went extinct during the LGM, and the refugium for the species was a relatively small area to the east of the Levantine Sea, corresponding to the Mediterranean coasts of present-day Syria, Lebanon, Israel, and northeastern and northwestern Egypt. Subsequently the species first repopulated Anatolia, diversified there, and afterwards expanded into the Caucasus, continental Europe and North Africa after the end of the LGM. The fossil record in Iberia and the ENM results indicate continuous presence of Miniopterus in this peninsula that most probably was related to the Maghrebian lineage during the LGM, which did not persist afterwards. Using our results combined with similar findings in previous studies, we propose a new paradigm explaining the general distribution of genetic diversity in Europe involving the recolonization of the continent, with the main contribution from refugial populations in Anatolia and the Middle East. The study shows how genetics and ENM approaches can complement each other in providing a more detailed picture of intraspecific evolution. (C) 2016 Elsevier Inc. All rights reserved., Research Fund of Bogazici University, Istanbul [08M104, 09S101, 11Y00P2]; Scientific and Technological Research Council of Turkey, TUBITAK [112T698]; Fundacao para a Ciencia e Tecnologia [IF/0497/2013]; IRCSET-Marie Curie International Mobility Fellowship in Science, Engineering and Technology; Ministry of Culture of the Czech Republic [DKRVO 00023272], We would like to thank Elizabeth Hemond and three anonymous reviewers for their comments on an earlier version of the manuscript. We also would like to thank Simos Demetropoulos, Petros Lymperakis, Dina Kovac, Vida Zrncic, Darija Josic, Sanja Drakulic, Tea Knapic, Yannis Kazoglou, Elena Papadatou, Xavier Gremillet, Monika Podgorelec, Peter Vallo, Thierry Disca, Vincent Prie, members of the Win-timdouine expedition (Morocco), Wassim M. Hizem, Pr. Said Nouira, and the "Direction generale des fork de Tunisie" for their assistance with the fieldwork. This study was supported by grants from the Research Fund of Bogazici University, Istanbul (08M104, 09S101 and 11Y00P2) and the Scientific and Technological Research Council of Turkey, TUBITAK (112T698) to RB, from Fundacao para a Ciencia e Tecnologia (research contract IF/0497/2013) to HR, from the IRCSET-Marie Curie International Mobility Fellowship in Science, Engineering and Technology to SJP, and from the Ministry of Culture of the Czech Republic (# DKRVO 00023272) to PB.

Mediterranean forests have been exploited by humans for centuries, but their present area and condition have been shaped mostly during the recent decades. Overgrazing resulting from national and EU policies, increasing fires and touristic development have led to the shrinkage of forest, threatening forest species. In this study, we examine the relation of Pipistrellus hanaki, a bat species limited to Cyrenaica, Libya and Crete, with the relict forests of Crete. Radiotracking of males (in autumn) and lactating females (in early summer) showed that P. hanaki uses a range of roost types, but Quercus forest stands and old tree cultivations are largely preferred for foraging, while open areas, young Cupressus stands and Mediterranean maquis are avoided. To unveil larger-scale patterns of habitat suitability by the species, we used Maxent to model its potential distribution on Crete using Chelsa Climatology. The resulted model showed high distribution probability around the mountains of central and western Crete, where native Cupressus, Pinus and Quercus stands are still present, and the climatic conditions are favourable. The strong dependence of this strictly southeast Mediterranean bat on forests and old tree cultivations stresses the need for conservation of these habitat types. In situ forest protection and environmental friendly livestock and farming practices are necessary to ensure the long-term survival of the species in Crete.

The interaction between agricultural production and wildlife can shape, and even condition, the functioning of both systems. In this study, we i) explored the degree to which a widespread European bat, namely the common bent-wing bat Miniopterus schreibersii, consumes crop-damaging insects at a continental scale, and ii) tested whether its dietary niche is shaped by the extension and type of agricultural fields. We employed a dual-primer DNA metabarcoding approach to characterize arthropod 16S and COI DNA sequences within bat faecal pellets collected across 16 Southern European localities, to first characterize the bat species’ dietary niche, second measure the incidence of agricultural pests across their ranges and third assess whether geographical dietary variation responds to climatic, landscape diversity, agriculture type and vegetation productivity factors. We detected 12 arthropod orders, among which lepidopterans were predominant. We identified >200 species, 44 of which are known to cause agricultural damage. Pest species were detected at all but one sampling site and in 94% of the analysed samples. Furthermore, the dietary diversity of M. schreibersii exhibited a negative linear relation with the area of intensive agricultural fields, thus suggesting crops restrict the dietary niche of bats to prey taxa associated with agricultural production within their foraging range. Overall, our results imply that M. schreibersii might be a valuable asset for biological pest suppression in a variety of agricultural productions and highlight the dynamic interplay between wildlife and agricultural systems., We thank the staff at the Danish National High-Throughput DNASequencing Centre for generating the sequencing data. Furthermore,we thank Kristine Bohmann and the three anonymous reviewers foredits and comments on the manuscript. AA was supported by The Danish Council for Independent Research (5051-00033), and OAwas supported by the Carlsberg Foundation’s Distinguished Postdoc-toral Fellowship (CF15-0619). SG was supported by a Marie Skło-dowska-Curie Individual Fellowship (655732)

Several recent impact studies reveal that in some localities industrial wind farms are associated with high numbers of bat fatalities. In Europe, most published studies have been conducted in the northwest, while bat diversity generally is much higher in the south of the continent. Here we provide evidence from a post-construction monitoring study conducted in north-eastern Greece between August 2009 and July 2010. Overall, 88 turbines from nine wind farms were intensively searched, and 181 dead and two injured bats were found in their proximity. The most frequently killed species were Nyctalus leisleri (n - 56), Pipistrellus pipistrellus/ P. pygmaeus (53), P. nathusii (35), Hypsugo savii (23) and N. noctula (10). Fatality rates were high from June to September. Most killed bats were adult males. Observed differences in the temporal pattern of fatalities among species may be associated with differences in their behaviour and distribution. Sex segregation with males at higher elevation, where the wind farm...

The analysis of social calls emitted by bats in the Pipistrellus genus has played an important role in assessing cryptic diversity and in investigating reproductive strategies and foraging ecology of these vespertilionids. Our study deals with social call structure of a recently discovered species, Pipistrellus hanaki. Because it shares a common ancestor with P. pygmaeus, we hypothesized that as in the other species from this group social call structure in P. hanaki retains the basic design observed in other bats from the same genus. We also hypothesized that as in other pipistrelles such calls fulfill context-dependent functions, being used by both foraging and mating bats for different purposes. However, we found that these calls have a structure rather different from that seen in most pipistrelles. Two different types of multi-harmonic, broad-band and long-duration social calls were identified, with distinctive temporal structures. Single component, frequency modulated-quasi constant frequency (FM-QCF) calls were emitted during summer at foraging sites where feeding buzzes were regularly produced. In autumn, when feeding buzz rates decreased and males caught at recording sites had prominent testes, a second type of single or multi-component calls with a fluctuating frequency over time was more frequent. Unlike the typical multi-component social calls recorded in several Pipistrellus species, our results suggest that social calls used by foraging or mating P. hanaki have markedly different structures. Because social calls of bats are speciesspecific and may function in reproductive isolation, we hypothesize that differentiation of social calls in P. hanaki has been associated with the divergence of this species from P. pygmaeus.

We describe here an automated surveillance system that was installed in a bearded vulture (Gypaetus barbatus) nest in Crete during 2003-2006 with the aim of studying siblicide in this species. The system caused only minimal levels of disturbance to the birds and was capable of operating autonomously for more than 1 week. It comprised (1) a nest monitoring subsystem (camera, microphone, battery with a charge controller and a transmitter together with an antenna), which was supported by a solar panel, and (2) a recording subsystem (antenna receiver, video signal controller and a remote controlled PC through a GSM modem) that compressed the audio-video signal and provided real time monitoring. Two-egg clutches with a 7-day laying and hatching interval were recorded while hatching asynchrony was also determined through an analysis of the bioacoustic data. Food delivery rate and number of feeds to the first chick increased after the hatching of its sibling although not significantly so. Of the aggressive interactions recorded, 98% were initiated by the dominant chick and resulted in an average of 246 ± 157 peckings per day. Supplementary feeding did not produce any significant correlation between feeding rate and sibling aggression. The second chick survived 5 days and died as a result of starvation accelerated by sibling aggression. Intervention measures aimed at increasing the survival chance of the second chick should be undertaken when the latter is 1-2 days old.

With more than 80% of the species global population breeding in Greece, Eleonora’s Falcon (Falco eleonorae) is reported to be the most important bird species in the country. A national population survey was conducted during the breeding seasons 2004–2006 in order to assess the species' breeding distribution and population size. This census was the first of its kind and was part of a global population survey, involving more than 80 field workers. Standard field protocols, described in the International Species Action Plan, and a GIS interactive database were developed. Data were stored and spatially explored in conjunction to historical information and past records. A total of about 17,660 falcons were counted or an estimated 12,300 breeding pairs, which were concentrated in six major regions, i.e., northeast Aegean, Sporades, east Cyclades, Antikythira, southwest Dodecanese and the satellite islets of eastern Crete. Compared to previous descriptions of breeding colonies and population status, spatial variation in site occupancy was detected with a population decline in one of the aforementioned regions and an apparent increase in all the rest. The results of this national survey, expected to be repeated every 10 years, provided guidance for reviewing the conservation status of Eleonora’s Falcon in Greece and baseline information for future monitoring of its population.