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Pampus minor is an important commercial fish. Due to the similarity of external morphological characteristics among the genus Pampus species, P. minor has often been identified as the juvenile group of both P. cinereus and P. argenteus. While little genetic background on this species is known, this study was based on control region sequences and provided the first evaluation of the genetic signature of 264 individuals of P. minor from 11 populations along the coasts of China and Malaysia. The results indicate high genetic haplotype diversity and low nucleotide diversity in this species. Additionally, two differentiated haplotype lineages were identified in the P. minor populations. However, phylogenetic structures corresponding to the geographical locations were unable to be established. Analysis of molecular variance identified a vast majority of the genetic variation occurring within populations. F-statistic test value (FST) of pairwise indicated that great differences existed between the Chinese and Malaysian P. minor populations. For the Chinese populations, the genetic differences were insignificant with the exception of the Xiamen population, which is a marginal population. During the late Pleistocene, a population expansion of P. minor occurred. These expanded populations originated from the glacial refugium in the South China Sea and then rapidly occupied and adapted to their new habitat. The results of this study provide genetic information for ensuring the protection and management of P. minor resources. [ABSTRACT FROM AUTHOR]

Pampus fishes (Perciformes: Stromateidae) are important commercial species and include six valid species in China. The southern lesser pomfret (Pampus minorLiu and Li, 1998) is a species of Pampus for which knowledge is incomplete. This report confirms the occurrence of P. minor in the coastal waters of Wenzhou, China, by analyzing five specimens of P. minor obtained in Wenzhou in March 2019. Morphometric measurements and meristic counts were performed. The diagnostic morphological features of these species were consistent with those of the neotype specimen of P. minor and sufficient for separating the examined specimens from other Pampus species. This study was based on the Cytochrome Oxidase Subunit 1 (CO1) gene, which was sequenced for the purpose of identification. The genetic distances between P. minor and other Pampus species ranged from 13.4% to 15.5%, and the mean genetic distance within the P. minor group was 0.2%. Given that P. minor has not been reported in this region, our findings represent the first record from the coastal waters of Wenzhou and extend the distribution range of this species to the central and northern East China Sea. The reason for the observed northward migration of some P. minor individuals from their northernmost recorded habitat (Taiwan Strait) might be global warming. The collection of additional specimens is needed to further study the geographic limits of P. minor and its presumed northern expansion. [ABSTRACT FROM AUTHOR]

Pampus minor is often mistakenly identified as the larva of Pampus argenteus or Pampus cinereus because of its small size. Despite its importance, studies on the population genetics of P. minor are not yet available. In the present study, the mitochondrial Cytb gene was employed to investigate the population genetics of P. minor collected along the coasts of China and Malaysia. The genetic diversity of all P. minor populations was moderate, and two major haplotype lineages were detected that were differentiated approximately 0.3 million years ago. These two haplotype lineages differed significantly in frequency distribution of Chinese and Malaysian populations, showing an imperfect geographical pedigree structure. Results of AMOVA also showed that the genetic differentiation was mainly among populations. According to the distribution of the haplotypes, an ancestral haplotype existed in both the Chinese population and the Malaysian population, further confirming that the Chinese and Malaysian P. minor populations originated from the same refuge in the South China Sea. A historical demographic analysis indicated that P. minor experienced a recent population expansion during the late Pleistocene period. Due to the need of P. minor to adapt to the diverse habitats, unique haplotypes were ultimately formed under the differing pressures of natural selection. This study is expected to provide a basis for future research of the population genetics of P. minor. [ABSTRACT FROM AUTHOR]

In this study, the complete mitochondrial genome sequence of Pampus minor has been determined by long polymerase chain reaction and primer walking methods. The mitochondrial genome is a circular molecule of 16,690 bp in length, including the typical structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and two non-coding regions (L-strand replication origin and control region), the gene contents of which are identical to those observed in most bony fishes. The overall base composition of the heavy strand shows 29.9% of T, 24.7% of C, 30.6% of A and 14.7% of G, with a slight A + T rich feature (60.5%). Within the control region, we identified the termination-associated sequence domain (TAS), and the conserved sequence block domains.

Understanding the genetic diversity and habitat adaptation patterns of different geographic populations of marine organisms is essential for the rational maintenance, development, and utilization of their resources. Pampus minor Liu and Li 1998 is an economically valuable marine fish species. To determine the population genetic structure and elucidate the genetic mechanism underlying the habitat adaptation of this species, restriction site-associated DNA sequencing (RAD-seq) was used to scan the whole genomes of three P. minor putative populations along the coast of China for single-nucleotide polymorphism (SNPs) and outlier SNPs. Our population genetic structure analysis based on 2388 SNPs and 731 outlier SNPs throughout the genome revealed no significant genetic differentiation among the three populations. Results suggested that the life-cycle characteristics of P. minor , its relatively large population sizes, and ocean current transport might have shaped its current genetic pattern. The annotation information of both assembled sequences (which included outlier SNPs) and candidate loci associated with adaptations indicated that genes involved in many processes, including ion exchange, osmotic pressure regulation, metabolism, and the immune response, have been very important in the adaptations of P. minor to its heterogeneous habitats. Against the background of increased human activities, increased fishing intensity, and destruction of marine habitats, the results of this study provide basic genetic information for the accurate division of protection units of P. minor. • It is the first time to scan the whole genomes of P. minor. • No significant genetic differentiation was detected among the three Chinese P. minor populations. • Genes involved in ion exchange, osmotic pressure regulation, metabolism, and immune response, have been important in the adaptations of P. minor to heterogeneous habitats. [ABSTRACT FROM AUTHOR]

In this study, the complete mitochondrial genome sequence of Pampus minor has been determined by long polymerase chain reaction and primer walking methods. The mitochondrial genome is a circular molecule of 16,690 bp in length, including the typical structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and two non-coding regions (L-strand replication origin and control region), the gene contents of which are identical to those observed in most bony fishes. The overall base composition of the heavy strand shows 29.9% of T, 24.7% of C, 30.6% of A and 14.7% of G, with a slight A + T rich feature (60.5%). Within the control region, we identified the termination-associated sequence domain (TAS), and the conserved sequence block domains. [ABSTRACT FROM AUTHOR]