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Background: Caries risk assessment and management from the cornerstone for successfully applying a minimum intervention dentistry philosophy in managing dental caries. Most applied work on caries prevention acknowledges the role of oral hygiene and dietary control strategies in reducing the prevalence and incidence of caries. What we need to address, however, is the necessity of initiating and sustaining behaviors that will ensure the implementation of strategies, that is "patient compliance.", Aims and Objectives: To present a novel method to monitor oral health-care behavior on a day-to-day basis and guide the parent-child team to set self-help goals. In addition, sustain them over time until the oral environment has positively changed and improved in caries risk status., Methodology: A mobile-based application and digital ecosystem have been developed to record daily data, motivate the user and present it as monthly and periodic graphic output. This supplements other caries risk assessment methods employed in recall follow-up to understand the changing oral environment., Results and Conclusion: Pilot trial results are promising and our mobile-based application seems to be definite adjuvant in improving and monitoring patient compliance., Competing Interests: None

CRISPR-Cas systems provide prokaryotes with acquired immunity against viruses and plasmids, but how these systems are regulated to prevent autoimmunity is poorly understood. Here, we show that in the S. pyogenes CRISPR-Cas system, a long-form transactivating CRISPR RNA (tracr-L) folds into a natural single guide that directs Cas9 to transcriptionally repress its own promoter (P cas ). Further, we demonstrate that P cas serves as a critical regulatory node. De-repression causes a dramatic 3,000-fold increase in immunization rates against viruses; however, heightened immunity comes at the cost of increased autoimmune toxicity. Using bioinformatic analyses, we provide evidence that tracrRNA-mediated autoregulation is widespread in type II-A CRISPR-Cas systems. Collectively, we unveil a new paradigm for the intrinsic regulation of CRISPR-Cas systems by natural single guides, which may facilitate the frequent horizontal transfer of these systems into new hosts that have not yet evolved their own regulatory strategies., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Aim: To evaluate microleakage of Nanoionomer (3M ESPE Ketac(™) N100 Light cured Nanoionomer Restorative) and Nanocomposite (3M ESPE Filtek(™) Z350 XT Universal Restorative) restorations, immersed in fruit drink, fresh fruit juice and soft drink., Study Design: Eighty caries free maxillary premolars extracted for orthodontic purpose were used for the study. Class V cavities were prepared and restored with Nanocomposite on buccal surface and Nanoionomer on the palatal surface. The teeth were thermocycled following the restoration. The experimental groups comprised of 72 teeth (3 groups comprising 24 teeth each for fruit drink, fresh fruit juice and soft drink), while remaining 8 formed the control group. Each of experimental group was further divided into three subgroups (low, medium and high immersion). The teeth were finally immersed in Rhodamine B dye, sectioned and evaluated under stereomicroscope. Statistical analyses used were Mann-Whitney test and ANOVA test., Results: The teeth showed statistically significant microleakage as the immersion regime increased. Soft drink group showed highest microleakage followed by fresh fruit juice and fruit drink. Nanocomposite exhibited more microleakage but the comparison was not statistically significant., Conclusion: The three beverages used in the study affected the microleakage of both restorative materials significantly. The microleakage scores increased as the frequency of the immersions increased. Soft drink caused highest microleakage followed by fresh fruit juice and fruit drink.

Aim: To assess the DMFT index of children with Special Health Care Needs (SHCN) in Navi Mumbai. To correlate the DMFT index with Streptococcus mutans count in the supragingival bacterial biofilm and with plaque pH., Materials and Methods: Dental examination of 158 patients aged 5-18 years was conducted to determine the DMFT/dmft index. Supragingival plaque samples were collected from the buccal surfaces of all teeth. The samples were inoculated in mitis salivarius bacitracin agar medium and incubated at 37°C for 48 h. Supragingival plaque was collected from interproximal sites of the molar area (preferably mandibular) for conducting plaque pH test. Chi-square test and Pearson's correlation were used to find the significance of the study parameters on categorical scale between the two groups., Results: The mean DMFT recorded was 4.90 ± 4.63 and the mean dmft recorded was 1.77 ± 3.14. Mean number of S. mutans colony-forming units found was 2.961 × 10(4). Mean plaque pH recorded was 6.2., Conclusion: No statistically significant correlation was found between the DMFT index with the number of S. mutans and plaque pH.

Objective: Early childhood caries is now affecting the children in dangerous proportions. There is a wide spread loss of the tooth material irrespective of the type of the carious lesion. Restoration of such lesions with a strong permanent bond between the dental tissues and the restorative dental materials would be a highly desirable requisite. Ultramorphological characterizations show that the interfacial morphology and the chemical characterization of the bonding systems appear to be strongly associated with each other and, therefore, observing and understanding the interfacial phenomenon and its quality would be of great importance in the selection of a dental adhesive for its use in pediatric restorative dentistry., Study Design: Human primary molars, which were indicated for extraction, for an array of reasons like caries, normal exfoliation, pathological root resorption, over-retained and serial extraction, were collected for the study purpose. Total number of teeth was then equally distributed into two subgroups, each namely A1 (Prime and Bond NT) and A2 (Xeno III)., Results: The type of etching pattern that was observed in group A1 (Prime and Bond NT) of Silverstone's type II compared to the Silverstone's type III observed in group A2 (Xeno III)., Conclusion: RESULTS of this study indicate that the use of an etchant separately followed by the application of the bonding system-Prime and Bond NT-would provide a better quality of adhesion thus improving the quality and longevity of the restoration done within the limits of enamel in primary dentition. How to cite this article: Mithiborwala SH, Chaugule V, Katge F, Poojari M, Pujari P, Pammi T. A Comparative Evaluation of the Efficacy of Etching by the Total Etch and Self-etch Dentin Bonding Systems in the Primary Teeth: An in vitro Study. Int J Clin Pediatr Dent 2015;8(1):30-36.

Dentists often find foreign bodies in the primary dentition of children who habitually place objects in their mouths. The objects are frequently embedded in exposures that result from carious or traumatic lesions or from endodontic procedures that have been left open for drainage. Such bodies are often detected on routine radiographs and, less frequently, during clinical examination. We report a case of a 6-year-old boy who had inadvertently embedded a screw in his mandibular right first primary molar and had forgotten about it until it became symptomatic. The screw was impacted in the exposed pulp chamber due to a large carious lesion in the affected molar. This case report considers the possible medical and dental consequences of placing foreign bodies in the mouth.