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Your search keyword '"Pamela Gallegos-Alcalá"' showing total 7 results
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7 results on '"Pamela Gallegos-Alcalá"'

1. Glycomacropeptide Protects against Inflammation and Oxidative Stress, and Promotes Wound Healing in an Atopic Dermatitis Model of Human Keratinocytes

Author

Pamela Gallegos-Alcalá, Mariela Jiménez, Daniel Cervantes-García, Laura Elena Córdova-Dávalos, Irma Gonzalez-Curiel, and Eva Salinas

Subjects
bioactive peptides, glycomacropeptide, atopic dermatitis, keratinocytes, cytoprotection, immunomodulation, Chemical technology, TP1-1185
Abstract

Keratinocytes are actively implicated in the physiopathology of atopic dermatitis (AD), a skin allergy condition widely distributed worldwide. Glycomacropeptide (GMP) is a milk-derived bioactive peptide generated during cheese making processes or gastric digestion. It has antiallergic and skin barrier restoring properties when it is orally administered in experimental AD. This study aimed to evaluate the effect of GMP on the inflammatory, oxidative, proliferative, and migratory responses of HaCaT keratinocytes in an in vitro AD model. GMP protected keratinocytes from death and apoptosis in a dose dependent manner. GMP at 6.3 and 25 mg/mL, respectively, reduced nitric oxide by 50% and 83.2% as well as lipid hydroperoxides by 27.5% and 45.18% in activated HaCaT cells. The gene expression of TSLP, IL33, TARC, MDC, and NGF was significantly downregulated comparably to control by GMP treatment in activated keratinocytes, while that of cGRP was enhanced. Finally, in an AD microenvironment, GMP at 25 mg/mL stimulated HaCaT cell proliferation, while concentrations of 0.01 and 0.1 mg/mL promoted the HaCaT cell migration. Therefore, we demonstrate that GMP has anti-inflammatory and antioxidative properties and stimulates wound closure on an AD model of keratinocytes, which could support its reported bioactivity in vivo.

Published
2023
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2. Protective Effect of Glycomacropeptide on the Inflammatory Response of U937 Macrophages

Author

Laura Elena Córdova-Dávalos, Daniel Cervantes-García, Maria Fernanda Ballona-Alba, Alejandra Santos-López, Alma Saraí Esquivel-Basaldúa, Pamela Gallegos-Alcalá, Mariela Jiménez, and Eva Salinas

Subjects
glycomacropeptide, sialic acid, human macrophage, inflammation, oxidative stress, SOCS3, Chemical technology, TP1-1185
Abstract

Macrophages play crucial roles in inflammation and oxidative stress associated with noncommunicable diseases, such as cardiovascular diseases, diabetes, and cancer. Glycomacropeptide (GMP) is a bioactive peptide derived from milk κ-casein that contains abundant sialic acid and has shown anti-inflammatory, antioxidative, anti-obesity, and anti-diabetic properties when is orally administered. The aim of this study was to evaluate the effect of GMP on the regulation of the inflammatory response in human macrophages and the participation of sialic acid in this activity. GMP pretreatment decreased by 35%, 35%, and 49% the production of nitrites, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α, respectively, in activated human macrophages U937. The same effect was obtained when cells were pretreated with asialo GMP, and no change on the gene expression of the lectins associated with the recognition of sialic acids, SIGLEC5, 7, and 9, was induced by GMP on macrophages, which suggests that sialic acid might not be involved in this immunoregulatory effect. Interestingly, GMP increased 8.9- and 3.5-fold the gene expression of the canonical anti-inflammatory protein SOCS3 and the antioxidant enzyme HMOX1, respectively, in U937 cells. Thus, GMP exerts anti-inflammatory and antioxidative activities on activated macrophages in a sialic acid-independent manner, which might be related to its in vivo reported bioactivity.

Published
2023
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3. The Keratinocyte as a Crucial Cell in the Predisposition, Onset, Progression, Therapy and Study of the Atopic Dermatitis

Author

Pamela Gallegos-Alcalá, Mariela Jiménez, Daniel Cervantes-García, and Eva Salinas

Subjects
keratinocyte, atopic dermatitis, allergic inflammatory response, keratinocyte differentiation, skin microbiome, in vitro atopic dermatitis models, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

The keratinocyte (KC) is the main functional and structural component of the epidermis, the most external layer of the skin that is highly specialized in defense against external agents, prevention of leakage of body fluids and retention of internal water within the cells. Altered epidermal barrier and aberrant KC differentiation are involved in the pathophysiology of several skin diseases, such as atopic dermatitis (AD). AD is a chronic inflammatory disease characterized by cutaneous and systemic immune dysregulation and skin microbiota dysbiosis. Nevertheless, the pathological mechanisms of this complex disease remain largely unknown. In this review, we summarize current knowledge about the participation of the KC in different aspects of the AD. We provide an overview of the genetic predisposing and environmental factors, inflammatory molecules and signaling pathways of the KC that participate in the physiopathology of the AD. We also analyze the link among the KC, the microbiota and the inflammatory response underlying acute and chronic skin AD lesions.

Published
2021
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4. Lactococcus lactis NZ9000 Prevents Asthmatic Airway Inflammation and Remodelling in Rats through the Improvement of Intestinal Barrier Function and Systemic TGF-β Production

Author

Eva Salinas, Mariela Jiménez, Leslie S Santoyo-Payán, Daniel Cervantes-García, Cesar Rivas-Santiago, Odila Saucedo-Cárdenas, María de Jesús Loera-Arias, Alicia Hernández-Mercado, Pamela Gallegos-Alcalá, and Roberto Montes de Oca-Luna

Subjects
Goblet cell, biology, business.industry, Immunology, Lactococcus lactis, Inflammation, General Medicine, Eosinophil, biology.organism_classification, Immunoglobulin E, Proinflammatory cytokine, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, Immune system, 030228 respiratory system, biology.protein, Immunology and Allergy, Medicine, medicine.symptom, 030223 otorhinolaryngology, business, Barrier function
Abstract

Introduction: The use of probiotics has been broadly popularized due to positive effects in the attenuation of aberrant immune responses such as asthma. Allergic asthma is a chronic respiratory disease characterized by airway inflammation and remodelling. Objective: This study was aimed to evaluate the effect of oral administration of Lactococcus lactis NZ9000 on asthmatic airway inflammation and lung tissue remodelling in rats and its relation to the maintenance of an adequate intestinal barrier. Methods: Wistar rats were ovalbumin (OVA) sensitized and challenged and orally treated with L. lactis. Lung inflammatory infiltrates and cytokines were measured, and remodelling was evaluated. Serum OVA-specific immunoglobulin (Ig) E levels were assessed. We also evaluated changes on intestinal environment and on systemic immune response. Results: L. lactis diminished the infiltration of proinflammatory leucocytes, mainly eosinophils, in the bronchoalveolar compartment, decreased lung IL-4 and IL-5 expression, and reduced the level of serum allergen-specific IgE. Furthermore, L. lactis prevented eosinophil influx, collagen deposition, and goblet cell hyperplasia in lung tissue. In the intestine, L. lactis-treated asthmatic rats increased Peyer’s patch and goblet cell quantity and mRNA expression of IgA, MUC-2, and claudin. Additionally, intestinal morphological alterations were normalized by L. lactis administration. Splenocyte proliferative response to OVA was abolished, and serum levels of transforming growth factor (TGF)-β were increased by L. lactis treatment. Conclusions: These findings suggest that L. lactis is a potential candidate for asthma prevention, and the effect is mediated by the improvement of intestinal barrier function and systemic TGF-β production.

Published
2020

6. The Keratinocyte as a Crucial Cell in the Predisposition, Onset, Progression, Therapy and Study of the Atopic Dermatitis

Author

Eva Salinas, Mariela Jiménez, Daniel Cervantes-García, and Pamela Gallegos-Alcalá

Subjects
Keratinocytes, Cell, Review, medicine.disease_cause, Skin Physiological Phenomena, Biology (General), Spectroscopy, Skin, integumentary system, atopic dermatitis, Microbiota, Disease Management, General Medicine, Atopic dermatitis, Combined Modality Therapy, Pathophysiology, Computer Science Applications, Chemistry, medicine.anatomical_structure, Disease Progression, Disease Susceptibility, Signal transduction, Keratinocyte, QH301-705.5, keratinocyte, Catalysis, Dermatitis, Atopic, Inorganic Chemistry, medicine, Animals, Humans, Genetic Predisposition to Disease, Physical and Theoretical Chemistry, Molecular Biology, QD1-999, Alleles, Epidermis (botany), Host Microbial Interactions, business.industry, keratinocyte differentiation, Organic Chemistry, Immune dysregulation, medicine.disease, Immunity, Innate, Immunology, skin microbiome, allergic inflammatory response, business, Dysbiosis, pharmacological therapy, Biomarkers, in vitro atopic dermatitis models
Abstract

The keratinocyte (KC) is the main functional and structural component of the epidermis, the most external layer of the skin that is highly specialized in defense against external agents, prevention of leakage of body fluids and retention of internal water within the cells. Altered epidermal barrier and aberrant KC differentiation are involved in the pathophysiology of several skin diseases, such as atopic dermatitis (AD). AD is a chronic inflammatory disease characterized by cutaneous and systemic immune dysregulation and skin microbiota dysbiosis. Nevertheless, the pathological mechanisms of this complex disease remain largely unknown. In this review, we summarize current knowledge about the participation of the KC in different aspects of the AD. We provide an overview of the genetic predisposing and environmental factors, inflammatory molecules and signaling pathways of the KC that participate in the physiopathology of the AD. We also analyze the link among the KC, the microbiota and the inflammatory response underlying acute and chronic skin AD lesions.

Published
2021

7. Lactococcus lactis NZ9000 Prevents Asthmatic Airway Inflammation and Remodelling in Rats through the Improvement of Intestinal Barrier Function and Systemic TGF-β Production

Author

Daniel, Cervantes-García, Mariela, Jiménez, César E, Rivas-Santiago, Pamela, Gallegos-Alcalá, Alicia, Hernández-Mercado, Leslie S, Santoyo-Payán, María de Jesús, Loera-Arias, Odila, Saucedo-Cardenas, Roberto, Montes de Oca-Luna, and Eva, Salinas

Subjects
Ovalbumin, Probiotics, Immunoglobulin E, Asthma, Rats, Lactococcus lactis, Disease Models, Animal, Transforming Growth Factor beta, Leukocytes, Airway Remodeling, Animals, Cytokines, Inflammation Mediators, Intestinal Mucosa
Abstract

The use of probiotics has been broadly popularized due to positive effects in the attenuation of aberrant immune responses such as asthma. Allergic asthma is a chronic respiratory disease characterized by airway inflammation and remodelling.This study was aimed to evaluate the effect of oral administration of Lactococcus lactis NZ9000 on asthmatic airway inflammation and lung tissue remodelling in rats and its relation to the maintenance of an adequate intestinal barrier.Wistar rats were ovalbumin (OVA) sensitized and challenged and orally treated with L. lactis. Lung inflammatory infiltrates and cytokines were measured, and remodelling was evaluated. Serum OVA-specific immunoglobulin (Ig) E levels were assessed. We also evaluated changes on intestinal environment and on systemic immune response.L. lactis diminished the infiltration of proinflammatory leucocytes, mainly eosinophils, in the bronchoalveolar compartment, decreased lung IL-4 and IL-5 expression, and reduced the level of serum allergen-specific IgE. Furthermore, L. lactis prevented eosinophil influx, collagen deposition, and goblet cell hyperplasia in lung tissue. In the intestine, L. lactis-treated asthmatic rats increased Peyer's patch and goblet cell quantity and mRNA expression of IgA, MUC-2, and claudin. Additionally, intestinal morphological alterations were normalized by L. lactis administration. Splenocyte proliferative response to OVA was abolished, and serum levels of transforming growth factor (TGF)-β were increased by L. lactis treatment.These findings suggest that L. lactis is a potential candidate for asthma prevention, and the effect is mediated by the improvement of intestinal barrier function and systemic TGF-β production.

Published
2020

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