42 results on '"Palmas M"'
Search Results
2. Efficacy and safety of NEPA, an oral combination of netupitant and palonosetron, for prevention of chemotherapy-induced nausea and vomiting following highly emetogenic chemotherapy: a randomized dose-ranging pivotal study
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Hesketh, P.J., Rossi, G., Rizzi, G., Palmas, M., Alyasova, A., Bondarenko, I., Lisyanskaya, A., and Gralla, R.J.
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- 2014
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3. Health status and 6 years survival of 552 90+ Italian sib-ships recruited within the EU Project GEHA (GEnetics of Healthy Ageing)
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Cevenini, E., Cotichini, R., Stazi, M. A., Toccaceli, V., Palmas, M. G., Capri, M., De Rango, F., Dato, S., Passarino, G., Jeune, B., Franceschi, C., and the GEHA Project Consortium
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- 2014
- Full Text
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4. Metabolic syndrome in the offspring of centenarians: focus on prevalence, components, and adipokines
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Ostan, R., Bucci, L., Cevenini, E., Palmas, M. G., Pini, E., Scurti, M., Vescovini, R., Caruso, C., Mari, D., Vitale, G., Franceschi, C., and Monti, D.
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- 2013
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5. Problematic social media use: Associations with health complaints among adolescents
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Marino, C., Lenzi, M., Canale, N., Pierannunzio, D., Dalmasso, P., Borraccino, A., Cappello, N., Lemma, P., Vieno, A., Nardone, P., Spinelli, A., Donati, S., Pizzi, E., Ciardullo, S., Andreozzi, S., Bucciarelli, M., de Mei, B., Cattaneo, C., Cavallo, F., Piraccini, G., Berchialla, P., Charrier, L., Sciannameo, V., Gaboardi, M., Santinello, M., Lazzeri, G., Giacchi, M. V., Pammolli, A., Simi, R., Galeone, D., Menzano, M. T., Vienna, A., Colleluori, C., Di Giacomo, M., Ranalli, E., Cauzillo, G., Mininni, M., Sorrentino, G., Azzarito, C., Cernuzio, A., la Rocca, M., Pugliese, A., Mazzarella, G., Angelini, P., Fridel, M., Carletti, C., Concina, F., Ronfani, L., Pani, P., Cairella, G., Bosca, L., Pancallo, M. T., Ferrando, G., Celata, C., Coppola, L., Lobascio, C., Gelmi, G., Crottogini, L., Velasco, V., de Introna, S., Giostra, G., Ciallella, M. L., Colitti, M., Paolitto, E., Caputo, M., Stingi, D., Pacella, P., Pasquale, P., Palmas, M. A., Murgia, A., Cernigliaro, A., Ferro, M. P., Scondotto, S., Aramini, L., Corridori, V., Cristofori, M., Sorbelli, D., Giovannini, G., Covarino, A. M., Michieletto, F., Bino, E., Zuccali, M. G., Fanolla, A., Weiss, S., Marino, C, Lenzi, M, Canale, N, Pierannunzio, D, Dalmasso, P, Borraccino, A, Cappello, N, Lemma, P, Vieno, A, Nardone, P, Spinelli, A, Donati, S, Pizzi, E, Ciardullo, S, Andreozzi, S, Bucciarelli, M, de Mei, B, Cattaneo, C, Cavallo, F, Piraccini, G, Berchialla, P, Charrier, L, Sciannameo, V, Gaboardi, M, Santinello, M, Lazzeri, G, Giacchi, M, Pammolli, A, Simi, R, Galeone, D, Menzano, M, Vienna, A, Colleluori, C, Di Giacomo, M, Ranalli, E, Cauzillo, G, Mininni, M, Sorrentino, G, Azzarito, C, Cernuzio, A, la Rocca, M, Pugliese, A, Mazzarella, G, Angelini, P, Fridel, M, Carletti, C, Concina, F, Ronfani, L, Pani, P, Cairella, G, Bosca, L, Pancallo, M, Ferrando, G, Celata, C, Coppola, L, Lobascio, C, Gelmi, G, Crottogini, L, Velasco, V, de Introna, S, Giostra, G, Ciallella, M, Colitti, M, Paolitto, E, Caputo, M, Stingi, D, Pacella, P, Pasquale, P, Palmas, M, Murgia, A, Cernigliaro, A, Ferro, M, Scondotto, S, Aramini, L, Corridori, V, Cristofori, M, Sorbelli, D, Giovannini, G, Covarino, A, Michieletto, F, Bino, E, Zuccali, M, Fanolla, A, and Weiss, S
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Male ,Adolescent ,Problematic use ,Psychosomatic symptom ,Adolescence ,Social media ,Cross-Sectional Studies ,Italy ,Psychosomatic symptoms ,Child ,Female ,Humans ,Internet Addiction Disorder ,Prevalence ,Self Report ,Social Media - Abstract
Objective. Problematic Social Media Use (PSMU) has an addictive potential for young users. The aim of this study was to show the prevalence of PSMU across Italian regions and its association with health complaints. Materials and methods. Data are gathered from the Italian 2018 Health Behaviour in School-aged Children survey using a representative sample of Italian adolescents aged 11, 13 and 15 years (50.6% males). Participants completed self-administered questionnaires assessing PSMU and health complaints. Results. PSMU affects 8.9% adolescents in Italy and the prevalence is quite consistent across regions. 13-year-olds girls showed the highest percentage of PSMU (13%). Problematic users of social media are more likely to report multiple somatic (OR = 1.84 [95% CI 1.82-1.85]) and psychological (OR = 2.60 [95% CI 2.58-2.63]) symptoms. Conclusions. PSMU represents a widespread problem in Italy. National prevention interventions are needed in order to promote a positive use of social media.
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- 2020
6. Dietary habits among Italian adolescents and their relation to socio-demographic characteristics
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Nardone P., Pierannunzio D., Ciardullo S., Lazzeri G., Cappello N., Spinelli A., Donati S., Pizzi E., Andreozzi S., Bucciarelli M., de Mei B., Cattaneo C., Cavallo F., Piraccini G., Berchialla P., Borraccino A., Charrier L., Dalmasso P., Lemma P., Sciannameo V., Vieno A., Canale N., Gaboardi M., Lenzi M., Marino C., Santinello M., Giacchi M. V., Pammolli A., Simi R., Galeone D., Menzano M. T., Vienna A., Colleluori C., Di Giacomo M., Ranalli E., Cauzillo G., Mininni M., Sorrentino G., Azzarito C., Cernuzio A., la Rocca M., Pugliese A., Mazzarella G., Angelini P., Fridel M., Carletti C., Concina F., Ronfani L., Pani P., Cairella G., Bosca L., Pancallo M. T., Ferrando G., Celata C., Coppola L., Lobascio C., Gelmi G., Crottogini L., Velasco V., de Introna S., Giostra G., Ciallella M. L., Colitti M., Paolitto E., Caputo M., Stingi D., Pacella P., Pasquale P., Palmas M. A., Murgia A., Cernigliaro A., Ferro M. P., Scondotto S., Aramini L., Corridori V., Cristofori M., Sorbelli D., Giovannini G., Covarino A. M., Michieletto F., Bino E., Zuccali M. G., Fanolla A., Weiss S., Nardone, P, Pierannunzio, D, Ciardullo, S, Lazzeri, G, Cappello, N, Spinelli, A, Donati, S, Pizzi, E, Andreozzi, S, Bucciarelli, M, de Mei, B, Cattaneo, C, Cavallo, F, Piraccini, G, Berchialla, P, Borraccino, A, Charrier, L, Dalmasso, P, Lemma, P, Sciannameo, V, Vieno, A, Canale, N, Gaboardi, M, Lenzi, M, Marino, C, Santinello, M, Giacchi, M, Pammolli, A, Simi, R, Galeone, D, Menzano, M, Vienna, A, Colleluori, C, Di Giacomo, M, Ranalli, E, Cauzillo, G, Mininni, M, Sorrentino, G, Azzarito, C, Cernuzio, A, la Rocca, M, Pugliese, A, Mazzarella, G, Angelini, P, Fridel, M, Carletti, C, Concina, F, Ronfani, L, Pani, P, Cairella, G, Bosca, L, Pancallo, M, Ferrando, G, Celata, C, Coppola, L, Lobascio, C, Gelmi, G, Crottogini, L, Velasco, V, de Introna, S, Giostra, G, Ciallella, M, Colitti, M, Paolitto, E, Caputo, M, Stingi, D, Pacella, P, Pasquale, P, Palmas, M, Murgia, A, Cernigliaro, A, Ferro, M, Scondotto, S, Aramini, L, Corridori, V, Cristofori, M, Sorbelli, D, Giovannini, G, Covarino, A, Michieletto, F, Bino, E, Zuccali, M, Fanolla, A, and Weiss, S
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Male ,vegetables ,eating behaviour ,Adolescent ,breakfast ,Feeding Behavior ,fruit ,Cross-Sectional Studies ,beverage ,Italy ,Socioeconomic Factors ,Humans ,Female ,Child ,Demography - Abstract
Objective. The aim is to describe dietary habits and their association with socio-demographic characteristics in a large nationally representative sample of Italian adolescents aged 11, 13 and 15 years. Materials and methods. Data from the 2018 Italian Health Behaviour in School-aged Children (HBSC) survey on 58,976 adolescents were analysed to determine eating habits. Logistic regression was used to investigate the association between incorrect dietary habits and potential predictors. Results. 38.3% of boys and 48.1% of girls skipped breakfast and 54.1% did not consume fruit and/or vegetables daily. 15.9% of boys and 11.3% of girls drank carbonated-sugary beverages at least once a day. Incorrect dietary habits were more common among boys, adolescents with lower socio-economic conditions, residents in Southern Italy and those spending more time watching TV. Italian adolescents were more likely to have incorrect dietary habits compared with those from most other countries involved in 2018 HBSC. Conclusions. Action is needed to improve dietary habits among adolescents.
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- 2020
7. Does the longevity of one or both parents influence the health status of their offspring?
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Gueresi, P., Miglio, R., Monti, D., Mari, D., Sansoni, P., Caruso, C., Bonafede, E., Bucci, L., Cevenini, E., Ostan, R., Palmas, M. G., Pini, E., Scurti, M., and Franceschi, C.
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- 2013
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8. COPPADIS-2015 (COhort of Patients with PArkinson's DIsease in Spain, 2015): an ongoing global Parkinson's disease project about disease progression with more than 1000 subjects included. Results from the baseline evaluation
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Garcia, D, Jesus, S, Aguilar, M, Planellas, L, Caldentey, J, Caballol, N, Legarda, I, Vara, J, Cabo, I, Manzanares, L, Aramburu, I, Rivera, M, Catalan, M, Diaz, L, Puente, V, Moreno, J, Borrue, C, Vila, B, Sauco, M, Vela, L, Escalante, S, Cubo, E, Padilla, F, Castrillo, J, Alonso, P, Losada, M, Ariztegui, N, Gaston, I, Kulisevsky, J, Gonzalez, M, Seijo, M, Martinez, J, Valero, C, Kurtis, M, de Fabregues-Boixar, O, Ardura, J, Jurczynska, C, Martinez-Martin, P, Mir, P, Astrid, D, Almeria, M, Canovas, A, Frech, F, Diaz, A, Arnaiz, S, Arribas, S, Vidondo, A, Lambrich, N, Bejr-Kasem, H, Estrada, M, Boti, M, Gonzalez, C, Lorenzo, A, Carrillo, F, Casas, E, Clavero, P, Fernandez, A, Foraster, A, Cuevas, A, Fonticoba, T, Diez-Fairen, M, Erro, E, Peyret, E, Guillan, N, Gamez, P, Gallego, M, Campos, C, Garre, M, Aloy, J, Garcia, B, Palmas, M, Toledo, G, Sola, M, Guardia, G, Horta-Barba, A, Infante, J, Labandeira, C, Labrador, M, Lacruz, F, Castro, M, Seoane, B, Macias, Y, Mata, M, Andres, G, Marti, M, McAfee, D, Meitin, M, del Barrio, C, Santiago, J, Casado, M, Dieguez, A, Nogueira, V, Amado, A, Ponte, S, Ordas, C, Pagonabarraga, J, Parees, I, Pascual-Sedano, B, Pastor, P, Fuertes, A, Noguera, R, Prats, M, Morlans, M, Rafales, N, Mendez, L, Perez, A, Roldan, F, De Arcos, M, Sanchez-Carpintero, M, Diez, G, Rodriguez, A, Santacruz, P, Rodriguez, J, Serarols, A, Pena, M, Castro, E, Tartari, J, Vargas, L, Gomez, R, Villanueva, C, Vives, B, Villar, M, and COPPADIS Study Grp
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motor fluctuations ,quality of life ,mood ,Parkinson's disease ,gait ,non-motor symptoms - Abstract
Background and purpose In Parkinson's disease (PD), the course of the disorder is highly variable between patients. Well-designed, prospective studies for identifying PD progression biomarkers are necessary. Our aim was to show the results of baseline evaluations of an ongoing global PD project, COPPADIS-2015 (Cohort of Patients with PArkinson's DIsease in Spain, 2015). Methods This was an observational, descriptive, nationwide study (Spain). The recruitment period ended in October 2017. Baseline evaluation included more than 15 validated scales and complementary studies in a subgroup of participants. Results In total, 1174 subjects from 35 centres were considered valid for baseline analysis: 694 patients (62.6 +/- 8.9 years old, 60.3% males), 273 caregivers (58.5 +/- 11.9 years old, 31.8% males) and 207 controls (61 +/- 8.3 years old, 49.5% males). The mean disease duration was 5.5 +/- 4.4 years. Hoehn and Yahr stage was 1 or 2 in 90.7% of the patients whilst 33.9% and 18.1% of them presented motor fluctuations and dyskinesias, respectively. The mean Non-Motor Symptoms Scale total score was 45.4 +/- 38.1, and 30.4% of the patients presented cognitive impairment, 16.1% major depression, 12.7% impulse control disorder, 7.2% compulsive behaviour, 57.2% pain and 13.2% falls. Compared to the control group, PD patients presented a significantly higher burden of non-motor symptoms and a worse quality of life. More than 300 subjects conducted complementary studies (serum biomarkers, genetic and neuroimaging). Conclusions Parkinson's disease is a complex disorder and different non-motor symptoms are frequently present and are more prevalent than in controls. In real clinical practice it is important to ask for them.
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- 2019
9. Non-motor symptoms burden, mood, and gait problems are the most significant factors contributing to a poor quality of life in non-demented Parkinson's disease patients: Results from the COPPADIS Study Cohort
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Garcia, D, Fonticoba, T, Castro, E, Borrue, C, Mata, M, Vila, B, Foraster, A, Sauco, M, Perez, A, Vela, L, Macias, Y, Escalante, S, Esteve, P, Villarroya, S, Cubo, E, Casas, E, Arnaiz, S, Padilla, F, Morlans, M, Mir, P, Martinez-Martin, P, Adarmes, A, Almeria, M, Losada, G, Canovas, A, Alonso-Frech, F, Arribas, S, Vidondo, A, Aguilar, M, Avila, M, Lambrich, N, Bejr-Kasem, H, Estrada, M, Boti, M, Gonzalez, C, Lopez, I, Caballol, N, Lorenzo, A, Carrillo, F, Catalan, M, Clavero, P, Fernandez, A, Cuevas, A, de Fabregues-Boixar, O, Diez-Fairen, M, Erro, E, Peyret, E, Guinan, N, Gamez, P, Gallego, M, Caldentey, J, Campos, C, Moreno, J, Gaston, I, Garre, M, Aloy, J, Gonzalez-Aramburu, I, Ardura, J, Garcia, B, Palmas, M, Toledo, G, Diaz, A, Sola, M, Guardia, G, Hernandez-Vara, J, Barba, A, Infante, J, Jesus, S, Kulisevsky, J, Kurtis, M, Labandeira, C, Labrador, M, Lacruz, F, Castro, M, Legarda, I, Ariztegui, N, Diaz, L, Manzanares, L, Seoane, B, Andres, G, Marti, M, Martinez-Castrillo, J, McAfee, D, Meitin, M, Gonzalez, M, del Barrio, C, Santiago, J, Casado, M, Dieguez, A, Nogueira, V, Amado, A, Ponte, S, Ordas, C, Pagonabarraga, J, Parees, I, Pascual-Sedano, B, Pastor, P, Fuertes, A, Noguera, R, Planellas, L, Fuster, J, Prats, M, Jurczynska, C, Puente, V, Rafales, N, Mendez, L, Roldan, F, De Arcos, M, Martinez, J, Alonso, P, Sanchez-Carpintero, M, Diez, G, Rodriguez, A, Santacruz, P, Rodriguez, J, Seijo, M, Serarols, A, Pena, M, Tartari, J, Vargas, L, Gomez, R, Villanueva, C, Vives, B, Villar, M, and Coppadis Study Grp
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Quality of life ,Parkinson's disease ,Mood ,Non-motor symptoms ,Gait ,humanities ,Motor fluctuations - Abstract
Objective: To identify factors related to a poor health-related and global quality of life (QoL) in a cohort of nondemented Parkinson's disease (PD) patients and compare to a control group. Methods: The data correspond to the baseline evaluation of the COPPADIS-2015 Study, an observational, 5-year follow-up, multicenter, evaluation study. Three instruments were used to assess QoL: (1) the 39-item Parkinson's disease Questionnaire (PDQ-39), (2) a subjective rating of global QoL (PQ-10), and (3) the EUROHIS-QOL 8-item index (EUROHIS-QOL8). Multiple linear regression methods were used to evaluate the direct impact of different variables on these QoL measures. Results: QoL was worse in PD patients (n = 692; 62.6 +/- 8.9 years old, 60.3% males) than controls (n = 206; 61 +/- 8.3 years old, 49.5% males): PDQ-39, 17.1 +/- 13.5 vs 4.4 +/- 6.3 (p < 0.0001); PQ-10, 7.3 +/- 1.6 vs 8.1 +/- 1.2 (p < 0.0001); EUROHIS-Q0L8, 3.8 +/- 0.6 vs 4.2 +/- 0.5 (p < 0.0001). A high correlation was observed between PDQ-39 and Non-Motor Symptoms Scale (NMSS) (r = 0.72; p < 0.0001), and PDQ-39 and Beck Depression Inventory-II (BDI-II) (r = 0.65; p < 0.0001). For health-related QoL (PDQ-39), non-motor symptoms burden (NMSS), mood (BDI-II), and gait problems (Freezing Of Gait Questionnaire [FOGQ]) provided the highest contribution to the model (beta = 0.32, 0.28, and 0.27, respectively; p < 0.0001); whereas mood and gait pro- blems contributed the most to global QoL (PQ-10, beta = -0.46 and -0.21, respectively; EUROHIS-QOL8, beta = - 0.44 and - 0.23, respectively). Conclusions: QoL is worse in PD patients than in controls. Mood, non-motor symptoms burden, and gait problems seem to be the most relevant factors affecting health-related and global perceived QoL in non-demented PD patients.
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- 2019
10. Multicycle Efficacy and Safety of Nepa, a Fixed-Dose Antiemetic Combination of Netupitant and Palonosetron, in Patients Receiving Chemotherapy of Varying Emetogenicity
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Aapro, M.S., primary, Gralla, R., additional, Karthaus, M., additional, Schwartzberg, L., additional, Rossi, G., additional, Rizzi, G., additional, Borroni, M.E., additional, Palmas, M., additional, Rugo, H.S., additional, and Jordan, K., additional
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- 2014
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11. Efficacy and safety of repeated dosing of netupitant, a neurokinin-1 receptor antagonist, in treating overactive bladder
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Haab, F., primary, Braticevici, B., additional, Krivoborodov, G., additional, Palmas, M., additional, Zufferli Russo, M., additional, and Pietra, C., additional
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- 2013
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12. Metabolic syndrome in the offspring of centenarians: focus on prevalence, components, and adipokines
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Ostan, R., primary, Bucci, L., additional, Cevenini, E., additional, Palmas, M. G., additional, Pini, E., additional, Scurti, M., additional, Vescovini, R., additional, Caruso, C., additional, Mari, D., additional, Vitale, G., additional, Franceschi, C., additional, and Monti, D., additional
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- 2012
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13. Age-Related Inflammation: the Contribution of Different Organs, Tissues and Systems. How to Face it for Therapeutic Approaches
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Cevenini, E., primary, Caruso, C., additional, Candore, G., additional, Capri, M., additional, Nuzzo, D., additional, Duro, G., additional, Rizzo, C., additional, Colonna-Romano, G., additional, Lio, D., additional, Carlo, D., additional, Palmas, M., additional, Scurti, M., additional, Pini, E., additional, Franceschi, C., additional, and Vasto, S., additional
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- 2010
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14. The influence of diabetes mellitus on primary open angle glaucoma perimetry
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Zucca, I., primary, Tanda, A., additional, Piras, V., additional, Mulas, S., additional, Mureddu, E., additional, Lecis, F., additional, Sulis, R., additional, Palmas, M. C., additional, Fossarello, M., additional, and Serra, A., additional
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- 2009
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15. 1484PD - Multicycle Efficacy and Safety of Nepa, a Fixed-Dose Antiemetic Combination of Netupitant and Palonosetron, in Patients Receiving Chemotherapy of Varying Emetogenicity
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Aapro, M.S., Gralla, R., Karthaus, M., Schwartzberg, L., Rossi, G., Rizzi, G., Borroni, M.E., Palmas, M., Rugo, H.S., and Jordan, K.
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- 2014
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16. Efficacy and safety of repeated dosing of netupitant, a neurokinin-1 receptor antagonist, in treating overactive bladder.
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Haab, F., Braticevici, B., Krivoborodov, G., Palmas, M., Zufferli Russo, M., and Pietra, C.
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Aim NK-1 receptors in sensory nerves, the spinal cord and bladder smooth muscle participate in complex sensory mechanisms that regulate bladder activity. This study was designed to assess the efficacy and safety of a new NK-1 receptor antagonist, netupitant, in patients with OAB. Methods This was a phase II, multicenter, double-blind study in which adults with OAB symptoms >6 months were randomized to receive 1 of 3 doses of netupitant (50, 100, 200 mg) or placebo once daily for 8 weeks. The primary efficacy endpoint was percentage change from baseline in average number of daily micturitions at week 8. Urinary incontinence, urge urinary incontinence (UUI), and urgency episodes were also assessed. Results The primary efficacy endpoint was similar in the treatment groups (−13.85 for placebo to −16.17 in the netupitant 200 mg group) with no statistically significant differences between netupitant and placebo. The same was true for most secondary endpoints although a significant difference for improvement in UUI episodes and a trend for the greatest decrease in urgency episodes were seen in the netupitant 100 mg group. Netupitant was well tolerated with most treatment emergent adverse events (AEs) being mild. While the overall incidence of AEs increased with netupitant dose, there was no evidence for this dose dependency based on relationship to treatment, intensity, or time to onset. Conclusions The study failed to demonstrate superiority of netupitant versus placebo in decreasing OAB symptoms, despite a trend favoring netupitant 100 mg. There were no safety concerns with daily administration of netupitant over 8 weeks. Neurourol. Urodynam. 33:335-340, 2014. © 2013 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]
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- 2014
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17. Association between dopamine receptor genes and migraine without aura in a Sardinian sample
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Del Zompo, M., primary, Cherchi, A., additional, Palmas, M. A., additional, Ponti, M., additional, Bocchetta, A., additional, Gessa, G. L., additional, and Piccardi, M. P., additional
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- 1998
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18. No evidence of association between dopamine D3 receptor gene and bipolar affective disorder
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Piccardi, M. Paola, primary, Severino, Giovanni, additional, Bocchetta, Alberto, additional, Palmas, M. Antonietta, additional, Ruiu, Stefania, additional, and Del Zompo, Maria, additional
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- 1997
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19. Xq28 polymorphic markers in bipolar disorder
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Piccardi, M P, primary, Mancini, M, additional, Palmas, M A, additional, Ruiu, S, additional, Bocchetta, A, additional, and Zompo, M Del, additional
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- 1995
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20. No evidence of association between dopamine D~3 receptor gene and bipolar disorder in a sardinian sample
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Severino, G., Palmas, M. A., Ruiu, S., Zompo, M. Del, and Piccardi, P.
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- 1996
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21. Accumulation of neutral lipids in peripheral blood mononuclear cells as a distinctive trait of Alzheimer patients and asymptomatic subjects at risk of disease
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Palmas Manuela, Zaru Cristina, Barberini Luigi, Saba Antonio, Pais Maria, Muçaka Nico, Brundu Annalisa, Angius Fabrizio, Cocco Pier, Abete Claudia, Diaz Giacomo, Mandas Antonella, Pani Alessandra, Putzu Paolo F, Mocali Alessandra, Paoletti Francesco, La Colla Paolo, and Dessì Sandra
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Medicine - Abstract
Abstract Background Alzheimer's disease is the most common progressive neurodegenerative disease. In recent years, numerous progresses in the discovery of novel Alzheimer's disease molecular biomarkers in brain as well as in biological fluids have been made. Among them, those involving lipid metabolism are emerging as potential candidates. In particular, an accumulation of neutral lipids was recently found by us in skin fibroblasts from Alzheimer's disease patients. Therefore, with the aim to assess whether peripheral alterations in cholesterol homeostasis might be relevant in Alzheimer's disease development and progression, in the present study we analyzed lipid metabolism in plasma and peripheral blood mononuclear cells from Alzheimer's disease patients and from their first-degree relatives. Methods Blood samples were obtained from 93 patients with probable Alzheimer's disease and from 91 of their first-degree relatives. As controls we utilized 57, cognitively normal, over-65 year-old volunteers and 113 blood donors aged 21-66 years, respectively. Data are reported as mean ± standard error. Statistical calculations were performed using the statistical analysis software Origin 8.0 version. Data analysis was done using the Student t-test and the Pearson test. Results Data reported here show high neutral lipid levels and increased ACAT-1 protein in about 85% of peripheral blood mononuclear cells freshly isolated (ex vivo) from patients with probable sporadic Alzheimer's disease compared to about 7% of cognitively normal age-matched controls. A significant reduction in high density lipoprotein-cholesterol levels in plasma from Alzheimer's disease blood samples was also observed. Additionally, correlation analyses reveal a negative correlation between high density lipoprotein-cholesterol and cognitive capacity, as determined by Mini Mental State Examination, as well as between high density lipoprotein-cholesterol and neutral lipid accumulation. We observed great variability in the neutral lipid-peripheral blood mononuclear cells data and in plasma lipid analysis of the subjects enrolled as Alzheimer's disease-first-degree relatives. However, about 30% of them tend to display a peripheral metabolic cholesterol pattern similar to that exhibited by Alzheimer's disease patients. Conclusion We suggest that neutral lipid-peripheral blood mononuclear cells and plasma high density lipoprotein-cholesterol determinations might be of interest to outline a distinctive metabolic profile applying to both Alzheimer's disease patients and asymptomatic subjects at higher risk of disease.
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- 2009
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22. Repurposing Pomalidomide as a Neuroprotective Drug: Efficacy in an Alpha-Synuclein-Based Model of Parkinson's Disease
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Maria Francesca Palmas, Anna Ena, Chiara Burgaletto, Maria Antonietta Casu, Giuseppina Cantarella, Ezio Carboni, Michela Etzi, Alfonso De Simone, Giuliana Fusco, Maria Cristina Cardia, Francesco Lai, Luca Picci, David Tweedie, Michael T. Scerba, Valentina Coroneo, Renato Bernardini, Nigel H. Greig, Augusta Pisanu, Anna R. Carta, Palmas, M. F., Ena, A., Burgaletto, C., Casu, M. A., Cantarella, G., Carboni, E., Etzi, M., De Simone, A., Fusco, G., Cardia, M. C., Lai, F., Picci, L., Tweedie, D., Scerba, M. T., Coroneo, V., Bernardini, R., Greig, N. H., Pisanu, A., Carta, A. R., Carta, Anna R [0000-0003-3104-9010], and Apollo - University of Cambridge Repository
- Subjects
Pharmacology ,Tumor Necrosis Factor-alpha ,Drug repositioning ,Parkinson Disease ,Neuroprotection ,Rats ,Thalidomide ,Alpha-synuclein ,Immunomodulation ,Substantia Nigra ,Disease Models, Animal ,Neuroprotective Agents ,Motor impairment ,Disease Progression ,Animals ,Cytokines ,Humans ,Pharmacology (medical) ,Neurology (clinical) ,Microglia ,Cytokine - Abstract
Marketed drugs for Parkinson’s disease (PD) treat disease motor symptoms but are ineffective in stopping or slowing disease progression. In the quest of novel pharmacological approaches that may target disease progression, drug-repurposing provides a strategy to accelerate the preclinical and clinical testing of drugs already approved for other medical indications. Here, we targeted the inflammatory component of PD pathology, by testing for the first time the disease-modifying properties of the immunomodulatory imide drug (IMiD) pomalidomide in a translational rat model of PD neuropathology based on the intranigral bilateral infusion of toxic preformed oligomers of human α-synuclein (H-αSynOs). The neuroprotective effect of pomalidomide (20 mg/kg; i.p. three times/week 48 h apart) was tested in the first stage of disease progression by means of a chronic two-month administration, starting 1 month after H-αSynOs infusion, when an already ongoing neuroinflammation is observed. The intracerebral infusion of H-αSynOs induced an impairment in motor and coordination performance that was fully rescued by pomalidomide, as assessed via a battery of motor tests three months after infusion. Moreover, H-αSynOs-infused rats displayed a 40–45% cell loss within the bilateral substantia nigra, as measured by stereological counting of TH + and Nissl-stained neurons, that was largely abolished by pomalidomide. The inflammatory response to H-αSynOs infusion and the pomalidomide treatment was evaluated both in CNS affected areas and peripherally in the serum. A reactive microgliosis, measured as the volume occupied by the microglial marker Iba-1, was present in the substantia nigra three months after H-αSynOs infusion as well as after H-αSynOs plus pomalidomide treatment. However, microglia differed for their phenotype among experimental groups. After H-αSynOs infusion, microglia displayed a proinflammatory profile, producing a large amount of the proinflammatory cytokine TNF-α. In contrast, pomalidomide inhibited the TNF-α overproduction and elevated the anti-inflammatory cytokine IL-10. Moreover, the H-αSynOs infusion induced a systemic inflammation with overproduction of serum proinflammatory cytokines and chemokines, that was largely mitigated by pomalidomide. Results provide evidence of the disease modifying potential of pomalidomide in a neuropathological rodent model of PD and support the repurposing of this drug for clinical testing in PD patients.
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- 2022
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23. Modeling Parkinson’s Disease Neuropathology and Symptoms by Intranigral Inoculation of Preformed Human α-Synuclein Oligomers
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Ignazia Mocci, Elzbieta Janda, Anna R. Carta, Saturnino Spiga, Alfonso De Simone, Anna Ena, Maria Antonietta Casu, Maria Scherma, Augusta Pisanu, Ezio Carboni, Giovanna Mulas, Maria Francesca Palmas, Giuliana Fusco, Paola Fadda, Laura Boi, Valentina Satta, Pisanu, Augusta [0000-0003-2633-8627], Janda, Elzbieta [0000-0002-6787-7291], Carta, Anna R [0000-0003-3104-9010], Apollo - University of Cambridge Repository, Boi, L., Pisanu, A., Palmas, M. F., Fusco, G., Carboni, E., Casu, M. A., Satta, V., Scherma, M., Janda, E., Mocci, I., Mulas, G., Ena, A., Spiga, S., Fadda, P., De Simone, A., Carta, A. R., Medical Research Council (MRC), and Commission of the European Communities
- Subjects
Male ,Parkinson's disease ,CLEARANCE ,Chemistry, Multidisciplinary ,Dopamine ,microglia ,Striatum ,TOXICITY ,neuroinflammation ,Rats, Sprague-Dawley ,lcsh:Chemistry ,0302 clinical medicine ,FIBRILS ,RAT MODEL ,Parkinson disease ,?-synuclein oligomers ,neurodegeneration ,motor deficits ,cognitive impairment ,Phosphorylation ,lcsh:QH301-705.5 ,Spectroscopy ,Neurons ,0303 health sciences ,α ,Microglia ,Chemistry ,Neurodegeneration ,General Medicine ,Recombinant Proteins ,3. Good health ,Computer Science Applications ,Substantia Nigra ,synuclein oligomers ,medicine.anatomical_structure ,Physical Sciences ,alpha-Synuclein ,Cytokines ,NEURONAL LOSS ,Life Sciences & Biomedicine ,Biochemistry & Molecular Biology ,α-synuclein oligomers ,0699 Other Biological Sciences ,Substantia nigra ,Motor deficit ,Neuroprotection ,Catalysis ,Article ,Inorganic Chemistry ,03 medical and health sciences ,Phagocytosis ,0399 Other Chemical Sciences ,medicine ,Animals ,Humans ,Physical and Theoretical Chemistry ,Molecular Biology ,Neuroinflammation ,030304 developmental biology ,Inflammation ,0604 Genetics ,Science & Technology ,Chemical Physics ,Pars compacta ,Dopaminergic Neurons ,Organic Chemistry ,IN-VITRO ,medicine.disease ,MICROGLIAL ACTIVATION ,Rats ,PATHOLOGY ,Disease Models, Animal ,nervous system ,lcsh:Biology (General) ,lcsh:QD1-999 ,PROTEIN OLIGOMERS ,Neuroscience ,030217 neurology & neurosurgery - Abstract
The accumulation of aggregated &alpha, synuclein (&alpha, Syn) is a hallmark of Parkinson&rsquo, s disease (PD). Current evidence indicates that small soluble &alpha, Syn oligomers (&alpha, SynOs) are the most toxic species among the forms of &alpha, Syn aggregates, and that size and topological structural properties are crucial factors for &alpha, SynOs-mediated toxicity, involving the interaction with either neurons or glial cells. We previously characterized a human &alpha, SynO (H-&alpha, SynO) with specific structural properties promoting toxicity against neuronal membranes. Here, we tested the neurotoxic potential of these H-&alpha, SynOs in vivo, in relation to the neuropathological and symptomatic features of PD. The H-&alpha, SynOs were unilaterally infused into the rat substantia nigra pars compacta (SNpc). Phosphorylated &alpha, Syn (p129-&alpha, Syn), reactive microglia, and cytokine levels were measured at progressive time points. Additionally, a phagocytosis assay in vitro was performed after microglia pre-exposure to &alpha, synOs. Dopaminergic loss, motor, and cognitive performances were assessed. H-&alpha, SynOs triggered p129-&alpha, Syn deposition in SNpc neurons and microglia and spread to the striatum. Early and persistent neuroinflammatory responses were induced in the SNpc. In vitro, H-&alpha, SynOs inhibited the phagocytic function of microglia. H-&alpha, synOs-infused rats displayed early mitochondrial loss and abnormalities in SNpc neurons, followed by a gradual nigrostriatal dopaminergic loss, associated with motor and cognitive impairment. The intracerebral inoculation of structurally characterized H-&alpha, SynOs provides a model of progressive PD neuropathology in rats, which will be helpful for testing neuroprotective therapies.
- Published
- 2020
24. Advances in modelling alpha-synuclein-induced Parkinson's diseases in rodents: Virus-based models versus inoculation of exogenous preformed toxic species
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Augusta Pisanu, Anna R. Carta, Maria Francesca Palmas, Ezio Carboni, Laura Boi, A. De Simone, Carta, A. R., Boi, L., Pisanu, A., Palmas, M. F., Carboni, E., and De Simone, A.
- Subjects
0301 basic medicine ,PFF ,Transgene ,Rodentia ,Fibril ,Synaptic vesicle ,Viral vector ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Gene duplication ,Animals ,Humans ,Alpha-synuclein ,Regulation of gene expression ,Rodent ,General Neuroscience ,AAV ,Parkinson Disease ,In vitro ,Cell biology ,030104 developmental biology ,chemistry ,Oligomer ,Viruses ,alpha-Synuclein ,030217 neurology & neurosurgery ,Parkinson model ,Virus vector - Abstract
Aggregates of alpha-synuclein (αSyn) have been described in Parkinson's disease (PD) patients, and recent evidence has suggested that the most toxic αSyn species in PD are small soluble aggregates including oligomers, prefibrils, protofibrils. The physiological function of αSyn is still highly debated, with a possible role in synaptic vesicle trafficking and release at the presynaptic compartment, and in the regulation of gene expression in the nucleus. Emerging evidence indicate that most of αSyn functions are related with the crucial ability to bind biological membranes, which is associated with structural conversion from a disordered monomer to an α-helical enriched structure. Conformational properties of αSyn can be modulated by a number of factors including post-translational modifications, gene duplication and triplication-driven overexpression, single point mutations, environmental changes, which affect membrane binding and the protein propensity to aggregate in toxic species. The recognized toxic role of αSyn in PD has laid the rational for purposing of αSyn-based, neuropathologically relevant preclinical models of PD. Different approaches have led to the establishment of transgenic models, viral vector-based models, and more recently models based on the intracerebral inoculation of exogenous αSyn preformed fibrils/oligomers. Here, we overview and compare viral vector-based models of αSyn overexpression and models obtained by direct intracerebral infusion of in vitro preformed αSyn species. The advantages and pitfalls associated with these different approaches are discussed.
- Published
- 2019
25. Clinical Image: Dystrophic Calcinosis in Systemic Sclerosis-Systemic Lupus Erythematosus Overlap Syndrome.
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Bazzocchi A, Palmas M, Bianchi G, and Aparisi Gómez MP
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- 2024
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26. What Is the Revision-free Survival of Resurfaced Allograft-prosthesis Composites for Proximal Humerus Reconstruction in Children With Bone Tumors?
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Errani C, Aiba H, Atherley A, Palmas M, Kimura H, Donati DM, and Manfrini M
- Abstract
Background: Reconstruction of the proximal humerus in children who undergo bone tumor resection is challenging because of patients' small bone size and possible limb length discrepancy at the end of skeletal growth due to loss of the physis. There are several options for proximal humerus reconstruction in children, such as clavicula pro humero, free vascularized fibula grafting, massive bone osteoarticular allografting, endoprostheses, and allograft-prosthesis composites, but no consensus exists on the best method for reconstruction. Resurfaced allograft-prosthesis composites could be an alternative surgical option, but little is known about the results of this surgical technique., Questions/purposes: (1) What are the complications and what is the survivorship free from reconstruction failure associated with resurfaced allograft-prosthesis composites in a small, single-center case series? (2) What Musculoskeletal Tumor Society scores do patients achieve after reconstructions with resurfaced allograft-prosthesis composites?, Methods: This study was a retrospective, single-arm case analysis in a single institution. We generally considered resurfaced allograft-prosthesis composites in children with malignant bone tumors involving the metaepiphysis of the proximal humerus in whom there was no evidence of joint contamination and in whom axillary nerve preservation was possible. Between 2003 and 2021, we treated 100 children (younger than 15 years) with bone tumors of the humerus. Thirty children (30%) with diaphyseal tumors (21 children) or distal tumors (9 children) were excluded. Among the potentially eligible children, 52 were not analyzed because they were treated with other procedures such as amputation, modular prostheses, cement spacers, free vascularized fibula grafting, and massive bone osteoarticular allografts. We included 18 children (26% of the potentially eligible children) who were treated with resurfaced allograft-prosthesis composites. There were 9 boys and 9 girls, with a median age of 10 years (range 4 to 15 years) at the time of diagnosis. A long stem (≥ 6 cm) in the resurfaced allograft-prosthesis composite was used in 9 children and a short stem (< 6 cm) was used in the remaining 9. One of the 18 children had a follow-up of less than 2 years. The median follow-up of the remaining 17 children was 4.7 years (range 2 to 19 years). The children' medical records were reviewed for clinical and functional outcomes. We performed a competing risk analysis to calculate the reconstruction failure-free survival of resurfaced allograft-prosthesis composites. Reconstruction failure was defined as removal of the implant or allograft because of implant loosening or breakage and allograft fracture or resorption. We analyzed the children's postoperative complications and functional outcomes at the end of the follow-up period using the Musculoskeletal Tumor Society functional scoring system., Results: The competing risk analysis revealed that reconstruction failure was 25% (95% confidence interval 7% to 40%) at 3 years, reaching a plateau. Four of 18 children underwent surgical revision with a new reconstruction. The reasons for reconstruction revision were resorption of the allograft at the proximal part (2 children) and fracture of the allograft (2 children). Reconstruction revision was performed in 3 of 9 children who underwent reconstruction with a short stem and in 1 of 9 children who underwent reconstruction with a long stem. Several children had other complications that did not result in removal of the allograft. Allograft resorption was observed in 4 of 18 children, but no additional surgical treatment was performed. Shoulder instability or subluxation was observed in 4 of 18 children, but only 1 child underwent surgery with a reverse shoulder arthroplasty without removal of the resurfaced allograft-prosthesis composite. Limited elbow motion because of plate impingement was observed in 1 child who underwent surgical cutting of the protruding distal part of the plate. Incomplete radial nerve palsy after surgery occurred in 1 child, with spontaneous resolution after 2 months. Screw loosening occurred in 2 children who underwent surgery with removal of loose screws. Two children had a nonunion at the graft-host bone junction; 1 child underwent surgery with bone grafting and refixation of the graft-host bone junction, and the other child with both nonunion and plate breakage was treated with bone grafting and refixation of the graft-host bone junction with a new plate. Among 17 children who had a follow-up longer than 2 years, the median Musculoskeletal Tumor Society functional score at the last follow-up interval was 23 of 30 (range 20 to 26); 1 child was considered to have an excellent result (functional score ≥ 26), 15 children were considered to have a good result (functional score 21 to 25), and 1 child was considered to have a fair result (functional score ≤ 20). The Musculoskeletal Tumor Society functional score did not change after excluding 4 children who underwent replacement of resurfaced allograft-prosthesis composites (24 of 30 [range 20 to 26]). The median angle of flexion of the shoulder was 40° (range 20º to 90°), and the median angle of abduction was 30° (range 20º to 90°)., Conclusion: Resurfaced allograft-prosthesis composites showed a high risk of complications, but not all complications resulted in removal of the reconstructed allograft. We used this technique mainly for very young children with small bones and for older children who underwent axillary nerve preservation. Although its success may be limited owing to a high risk of complications, a resurfaced allograft-prosthesis composite could be an alternative surgical option in order to preserve the bone stock and achieve good functional outcomes in very young children. We recommend using a long-stem resurfaced allograft-prosthesis composite, which may reduce the risk of complications., Level of Evidence: Level IV, therapeutic study., Competing Interests: Each author certifies that there are no funding or commercial associations (consultancies, stock ownership, equity interest, patent/licensing arrangements, etc.) that might pose a conflict of interest in connection with the submitted article related to the author or any immediate family members. All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research® editors and board members are on file with the publication and can be viewed on request., (Copyright © 2024 by the Association of Bone and Joint Surgeons.)
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- 2024
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27. Delimiting CD34+ Stromal Cells/Telocytes Are Resident Mesenchymal Cells That Participate in Neovessel Formation in Skin Kaposi Sarcoma.
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Díaz-Flores L, Gutiérrez R, González-Gómez M, García MDP, Palmas M, Carrasco JL, Madrid JF, and Díaz-Flores L Jr
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- Humans, Antigens, CD34 metabolism, Endothelial Cells metabolism, Neovascularization, Pathologic metabolism, Neovascularization, Pathologic pathology, Sarcoma, Kaposi pathology, Skin Neoplasms pathology, Stromal Cells metabolism, Stromal Cells pathology, Telocytes metabolism, Telocytes pathology
- Abstract
Kaposi sarcoma (KS) is an angioproliferative lesion in which two main KS cell sources are currently sustained: endothelial cells (ECs) and mesenchymal/stromal cells. Our objective is to establish the tissue location, characteristics and transdifferentiation steps to the KS cells of the latter. For this purpose, we studied specimens of 49 cases of cutaneous KS using immunochemistry and confocal and electron microscopy. The results showed that delimiting CD34+ stromal cells/Telocytes (CD34+SCs/TCs) in the external layer of the pre-existing blood vessels and around skin appendages form small convergent lumens, express markers for ECs of blood and lymphatic vessels, share ultrastructural characteristics with ECs and participate in the origin of two main types of neovessels, the evolution of which gives rise to lymphangiomatous or spindle-cell patterns-the substrate of the main KS histopathological variants. Intraluminal folds and pillars (papillae) are formed in the neovessels, which suggests they increase by vessel splitting (intussusceptive angiogenesis and intussusceptive lymphangiogenesis). In conclusion, delimiting CD34+SCs/TCs are mesenchymal/stromal cells that can transdifferentiate into KS ECs, participating in the formation of two types of neovessels. The subsequent growth of the latter involves intussusceptive mechanisms, originating several KS variants. These findings are of histogenic, clinical and therapeutic interest.
- Published
- 2023
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28. The role of conventional radiography to assess the outcome of oncologic skeletal reconstructions of lower limbs aided by vascularized fibular autograft.
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Spinnato P, Colangeli M, Crombé A, Scalas G, Palmas M, Frisoni T, Errani C, Mercatelli D, Saenz Mesen L, Campanacci L, Donati DM, and Manfrini M
- Subjects
- Male, Humans, Adolescent, Autografts, Retrospective Studies, Radiography, Fibula diagnostic imaging, Fibula surgery, Lower Extremity, Osteosarcoma diagnostic imaging, Osteosarcoma surgery, Bone Neoplasms diagnostic imaging, Bone Neoplasms surgery
- Abstract
Introduction: Vascularized fibular autografts (VFA) are used in the oncologic skeletal reconstructions of long bones, alone or combined with massive bone allografts (MBA). Data regarding the role of imaging in assessing these complex skeletal reconstructions are lacking, and have mainly focused on Computed Tomography (CT). Our aim was to evaluate if early conventional radiography (CR) findings are correlated with the outcome of these skeletal reconstructions., Materials and Methods: All consecutive patients who underwent oncologic resection of lower limbs long bones followed by VFA reconstruction were included in this single-center retrospective study. We compared the CR obtained immediately after surgery with the CR at the 6-month control, as well as the CR at 6 months with the CT at 6 months when available. The following scores were assigned to the VFA: 0 (unchanged), 1 (osteopenia-cortical bone thinning), 2 (increase in bone density-cortical thickening). We then investigated whether this score correlated with the implant outcome within 12 months (optimal integration, suboptimal integration, integration requiring further surgery or lack of integration) using Kaplan-Meier and Cox regression analyses, considering the occurrence of integration and the duration time before the surgical removal of the whole bone reconstruction., Results: Forty-five patients were included (32 men [71.1%], mean age 14.6 years), 26 affected by osteosarcoma, 14 by Ewing sarcoma, 3 by adamantinoma and 2 operated for the failure of previous reconstructions for bone sarcoma. VFA changes on 6-month CR were significantly associated with optimal integration of the implants (log-rank P = 0.0137, multivariate Hazard ratio = 7.62, 95% confidence interval = 1.13-51.25). None of the other clinical and surgical features were associated with the implant outcome. The findings on 6-month CR and CT follow-up were not significantly different. CT at 6 months was available in 36 patients (80.0%)., Conclusion: The assessment of VFA morphological changes on CR performed at 6 months can predict the outcome of the skeletal implant. This data should be considered for clinical decision-making, selecting patients requiring additional images (CT), and possible subsequent revision surgical procedures., Competing Interests: Declaration of competing interest We confirm that that the article is not under consideration for publication elsewhere. Each author have participated actively and sufficiently in any submission to take public responsibility for its content. Each author approved the final version of this publication. That the authors and their institutions have no conflicts of interest of any kind to declare., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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29. Nanocrystals as an effective strategy to improve Pomalidomide bioavailability in rodent.
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Cristina Cardia M, Francesca Palmas M, Casula L, Pisanu A, Marceddu S, Valenti D, Sinico C, Pini E, Scerba MT, Tweedie D, Greig NH, Rosa Carta A, and Lai F
- Subjects
- Animals, Biological Availability, Male, Rats, Rats, Sprague-Dawley, Rodentia, Solubility, Tumor Necrosis Factor Inhibitors, Nanoparticles chemistry, Thalidomide analogs & derivatives
- Abstract
Pomalidomide (POM) is an FDA-approved immunomodulatory imide drug (IMiDs) an it is effectively used in the treatment of multiple myeloma. IMiDs are analogs of the drug thalidomide and they have been repurposed for the treatment of several diseases such as psoriatic arthritis and Kaposi Sarcoma. In recent years, IMiDs have been also evaluated as a new treatment for neurological disorders with an inflammatory and neuroinflammatory component. POM draws particular interest for its potent anti-TNF-α activity at significantly lower concentrations than the parent compound thalidomide. However, POM's low water solubility underpins its low gastrointestinal permeability resulting in irregular and poor absorption. The purpose of this work was to prepare a POM nanocrystal-based formulation that could efficiently improve POM's plasma and brain concentration after intraperitoneal injection. POM nanocrystals prepared as a nanosuspension by the media milling method showed a mean diameter of 219 nm and a polydispersity index of 0.21. POM's nanocrystal solubility value (22.97 µg/mL) in phosphate buffer was about 1.58 folds higher than the POM raw powder. Finally, in vivo studies conducted in adult Male Sprague-Dawley rats indicated that POM nanocrystal ensured higher and longer-lasting drug levels in plasma and brain when compared with POM coarse suspension., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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30. Intercalary reconstruction of long bones by massive allograft: Comparison of construct stability ensured by three different host-graft junctions and two types of fixations in a synthetic femur model.
- Author
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Baleani M, Erani P, Blaise M, Fognani R, Palmas M, and Manfrini M
- Abstract
An intercalary segmental allograft is an option for limb salvage in bone tumours. Stable and congruent intercalary reconstructions are a prerequisite for achieving host-graft union. However, a too rigid fixation could increase the risk of late complications correlated with negative bone remodelling. This study compared the reconstruction stiffness achieved by three different host-graft junctions, namely, end-to-end, modified step-cut, and taper. A low-stiffness bone plate was used as the fixation method, except for the taper junction where a low-stiffness intramedullary nail was also used to investigate the effects of different types of fixation on construct stiffness. Composite femora were tested under four loading conditions to determine coronal and sagittal bending stiffness, as well as torsional stiffness in opposite directions. Stiffness values were expressed as a percentage of intact host bone stiffness (%IBS). While a reduction of coronal bending stiffness was found with taper junctions (76%IBS) compared with the high values ensured by end-to-end (96%IBS) and modified step-cut junctions (92%IBS), taper junctions significantly increased stiffness under sagittal bending and torsion in intra- and extra-direction: end-to-end 29%IBS, 7%IBS, 7%IBS, modified step-cut 38%IBS, 20%IBS, 21%IBS, and taper junction 52%IBS, 55%IBS, 56%IBS, respectively. Construct stiffness with taper junctions was decreased by 11-41%IBS by replacing the bone plate with an intramedullary nail. Taper junctions can be an alternative to achieve intercalary reconstructions with more homogeneous and, in three out of four loading conditions, significantly higher construct stability without increasing bone plate stiffness. The risk of instability under high torsional loads increases when taper junctions are associated with a low-stiffness intramedullary nail., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Baleani, Erani, Blaise, Fognani, Palmas and Manfrini.)
- Published
- 2022
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31. Posterior Leg Pain: Understanding Soleus Muscle Injuries.
- Author
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Siedi AF, Rolon AU, Bernard N, Bernasconi J, Palmas M, Couto DA, and Pascual TA
- Subjects
- Back Pain, Humans, Muscle, Skeletal diagnostic imaging, Leg, Muscular Diseases pathology
- Abstract
Soleus muscle injuries are frequently unrecognized, representing a common cause of sports inactivity. This is mainly because little is known about the anatomy of the soleus muscle and the clinical manifestations of injury. Unlike other muscles, the soleus muscle has a complex myoconnective structure with three intramuscular tendons, which makes the interpretation of muscle pathologic conditions challenging. Soleus muscle injuries can be acute or chronic and are usually considered to be a minor discomfort by both the patient and the sports medicine physician, leading to a relatively quick return to sporting activity with a high risk for reinjury. The authors review the soleus muscle anatomy and the importance of being familiar with the most frequent locations of injuries, which are fundamental aspects that every radiologist should understand to avoid underdiagnosis. The role of imaging, the clinical manifestations of injuries, and the differential diagnoses are key aspects to know when evaluating posterior leg pain. The online slide presentation from the RSNA Annual Meeting is available for this article.
© RSNA, 2022.- Published
- 2022
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32. Advances in modelling alpha-synuclein-induced Parkinson's diseases in rodents: Virus-based models versus inoculation of exogenous preformed toxic species.
- Author
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Carta AR, Boi L, Pisanu A, Palmas MF, Carboni E, and De Simone A
- Subjects
- Animals, Humans, Rodentia, Viruses, Parkinson Disease genetics, alpha-Synuclein genetics
- Abstract
Aggregates of alpha-synuclein (αSyn) have been described in Parkinson's disease (PD) patients, and recent evidence has suggested that the most toxic αSyn species in PD are small soluble aggregates including oligomers, prefibrils, protofibrils. The physiological function of αSyn is still highly debated, with a possible role in synaptic vesicle trafficking and release at the presynaptic compartment, and in the regulation of gene expression in the nucleus. Emerging evidence indicate that most of αSyn functions are related with the crucial ability to bind biological membranes, which is associated with structural conversion from a disordered monomer to an α-helical enriched structure. Conformational properties of αSyn can be modulated by a number of factors including post-translational modifications, gene duplication and triplication-driven overexpression, single point mutations, environmental changes, which affect membrane binding and the protein propensity to aggregate in toxic species. The recognized toxic role of αSyn in PD has laid the rational for purposing of αSyn-based, neuropathologically relevant preclinical models of PD. Different approaches have led to the establishment of transgenic models, viral vector-based models, and more recently models based on the intracerebral inoculation of exogenous αSyn preformed fibrils/oligomers. Here, we overview and compare viral vector-based models of αSyn overexpression and models obtained by direct intracerebral infusion of in vitro preformed αSyn species. The advantages and pitfalls associated with these different approaches are discussed., Competing Interests: Declaration of Competing Interest None., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
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33. Preventing chemotherapy-induced nausea and vomiting in patients with lung cancer: efficacy of NEPA (netupitant-palonosetron), the first combination antiemetic.
- Author
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Hesketh PJ, Palmas M, and Nicolas P
- Subjects
- Adult, Aged, Double-Blind Method, Humans, Male, Middle Aged, Nausea chemically induced, Palonosetron pharmacology, Pyridines pharmacology, Vomiting chemically induced, Antiemetics therapeutic use, Nausea prevention & control, Palonosetron therapeutic use, Pyridines therapeutic use, Vomiting prevention & control
- Abstract
Purpose: Patients receiving platinum-based chemotherapy are at high risk of chemotherapy-induced nausea and vomiting (CINV), a distressing side effect of treatment. This post-hoc subgroup analysis of two pivotal trials evaluated the efficacy of NEPA in preventing CINV in subsets of patients with lung cancer who received cisplatin or carboplatin., Methods: In each study, the efficacy endpoints complete response (CR; defined as no emetic episodes and no rescue medication) and no significant nausea (NSN; defined as a score of < 25 mm on a visual analog scale of 0-100 mm) during the acute (0-24 h), delayed (25-120 h), and overall (0-120 h) phases post-chemotherapy in cycle 1 (study 1) and cycles 1-4 (study 2) were assessed. Safety was evaluated by recording treatment-emergent adverse events (AEs) and treatment-related AEs., Results: NEPA treatment resulted in high CR rates across the acute, delayed, and overall phases (cisplatin: > 88% overall CR; carboplatin: > 75% overall CR), with higher CR rates for NEPA-treated patients than those receiving palonosetron; moreover, CR rates were sustained over multiple chemotherapy cycles (> 75%). High rates of NSN observed during cycle 1 (> 79%) were also maintained over multiple chemotherapy cycles. NEPA was well tolerated in all patients., Conclusions: NEPA appears to be effective and well tolerated in patients with lung cancer receiving platinum-based chemotherapy, across the acute, delayed, and overall phases and throughout multiple cycles. As a highly effective oral combination antiemetic agent administered as a single dose once per cycle, NEPA may offer a convenient, simplified prophylactic antiemetic.
- Published
- 2018
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34. Safety and efficacy of NEPA, an oral fixed combination of netupitant and palonosetron, in older patients.
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Aapro M, Jordan K, Gralla RJ, Rizzi G, Rossi G, Palmas M, Alyasova AV, Lisyanskaya AS, Bošnjak SM, and Hesketh PJ
- Subjects
- Aged, Anthracyclines administration & dosage, Cyclophosphamide administration & dosage, Drug Combinations, Female, Humans, Male, Nausea chemically induced, Treatment Outcome, Vomiting chemically induced, Antiemetics therapeutic use, Antineoplastic Combined Chemotherapy Protocols adverse effects, Isoquinolines therapeutic use, Nausea prevention & control, Pyridines therapeutic use, Quinuclidines therapeutic use, Vomiting prevention & control
- Abstract
Objectives: Prevention of chemotherapy-induced nausea and vomiting is critical in older patients with cancer. NEPA is an oral fixed combination of netupitant 300mg, a new NK
1 receptor antagonist (RA), and palonosetron 0.5mg, a pharmacologically distinct 5-HT3 RA. This retrospective analysis evaluated the efficacy and safety of NEPA in older patients., Methods: Patients aged ≥65 and ≥70years from one phase II and two phase III trials were considered. Chemotherapy-naive patients with malignant tumors were treated with anthracycline-cyclophosphamide (AC), non-AC-based moderately emetogenic chemotherapy (non-AC MEC), or highly emetogenic chemotherapy (HEC). Following single-dose NEPA, patients received oral dexamethasone on day 1 (AC and non-AC MEC) or days 1-4 (HEC). Efficacy was evaluated through complete response (CR) in cycle 1. Safety was evaluated by AEs and ECGs. Data were summarized by descriptive statistics., Results: Overall, 214 patients were ≥65years and 80 were ≥70years. A higher CR was observed in older patients versus the total population; in the acute phase >90% of patients ≥65years experienced CR. Efficacy was maintained over multiple cycles of chemotherapy. No significant nausea rates were generally higher in the older patients versus total population. Similar rates of AEs in the first treatment cycle were reported for patients ≥65years, ≥70years, and total population (72.9% vs 67.5% vs 70.0%, respectively). No cardiac safety concerns were raised., Conclusion: NEPA is highly effective in older patients receiving MEC or HEC regimens. NEPA is also well tolerated, demonstrating suitability for use in older patients who may have comorbidities., (Copyright © 2016 Elsevier Inc. All rights reserved.)- Published
- 2017
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35. Safety of an Oral Fixed Combination of Netupitant and Palonosetron (NEPA): Pooled Data From the Phase II/III Clinical Program.
- Author
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Aapro M, Hesketh PJ, Jordan K, Gralla RJ, Rossi G, Rizzi G, and Palmas M
- Subjects
- Adult, Aged, Biomarkers, Pharmacological, Dexamethasone administration & dosage, Dexamethasone adverse effects, Drug Combinations, Drug Therapy, Drug-Related Side Effects and Adverse Reactions pathology, Female, Humans, Isoquinolines adverse effects, Male, Middle Aged, Nausea chemically induced, Nausea pathology, Nausea prevention & control, Neoplasms complications, Neoplasms pathology, Neurokinin-1 Receptor Antagonists therapeutic use, Pyridines adverse effects, Quinuclidines adverse effects, Randomized Controlled Trials as Topic, Serotonin 5-HT3 Receptor Antagonists therapeutic use, Vomiting chemically induced, Vomiting pathology, Vomiting prevention & control, Antiemetics therapeutic use, Drug-Related Side Effects and Adverse Reactions prevention & control, Isoquinolines therapeutic use, Neoplasms drug therapy, Pyridines therapeutic use, Quinuclidines therapeutic use
- Abstract
Background: Standard prophylaxis for chemotherapy-induced nausea and vomiting (CINV) with highly emetogenic and anthracycline-cyclophosphamide-based chemotherapy includes a 5-hydroxytryptamine-3 receptor antagonist, a neurokinin-1 receptor antagonist (NK1RA), and corticosteroid therapy. NEPA is a fixed combination of netupitant and palonosetron. The primary objective of this analysis was to document the safety profile, including cardiac safety, of NEPA + dexamethasone in comparison with current therapies across all phase II/III trials., Materials and Methods: This pooled analysis was based on data from 3,280 patients in 4 randomized, double-blind clinical trials. Patients were categorized into 1 of 3 pooled groups on the basis of actual treatment received: NEPA + dexamethasone, palonosetron + dexamethasone, and aprepitant + ondansetron/palonosetron + dexamethasone. Safety was assessed by number and frequency of adverse events (AEs) and changes from baseline electrocardiogram measures., Results: Most patients were female and younger than 65 years of age. Demographic characteristics varied among studies and pooled groups. Frequencies of treatment-emergent AEs (TEAEs) and treatment-related AEs (TRAEs) were similar across groups. TEAEs were mostly mild and consistent with expected chemotherapy and disease-related AEs (hematologic events, hair loss, general weakness). TRAEs in ≥2% of patients were headache and constipation. Frequencies of cardiac TEAEs were similar across groups, with QT prolongation (1.6%), tachycardia (1.1%), and dyspnea (0.9%) the most common. Serious cardiac TEAEs were rare., Conclusion: NEPA was well-tolerated, with an AE profile as expected for the regimen. Sample size, demographic characteristics, study design, chemotherapy, and antiemetic regimen differences across the four studies may have contributed to differences in frequencies of neutropenia and alopecia. Adding an NK1RA to a CINV prophylaxis regimen can improve outcomes without additional toxicity., Implications for Practice: Supportive care for cancer should ideally be efficacious, convenient, and well-tolerated. There have been concerns about cardiac safety with current antiemetic prophylactic agents, namely dolasetron and ondansetron. This pooled safety analysis demonstrates that the new oral fixed combination therapy NEPA can be safely added to an antiemetic regimen without increased toxicity., (©AlphaMed Press.)
- Published
- 2016
- Full Text
- View/download PDF
36. Accumulation of neutral lipids in peripheral blood mononuclear cells as a distinctive trait of Alzheimer patients and asymptomatic subjects at risk of disease.
- Author
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Pani A, Mandas A, Diaz G, Abete C, Cocco PL, Angius F, Brundu A, Muçaka N, Pais ME, Saba A, Barberini L, Zaru C, Palmas M, Putzu PF, Mocali A, Paoletti F, La Colla P, and Dessì S
- Subjects
- Adult, Aged, Aged, 80 and over, Biomarkers, Cholesterol, HDL blood, Female, Humans, Intelligence Tests, Male, Middle Aged, Plasma chemistry, Severity of Illness Index, Statistics as Topic, United States, Young Adult, Alzheimer Disease pathology, Leukocytes, Mononuclear metabolism, Phospholipids metabolism
- Abstract
Background: Alzheimer's disease is the most common progressive neurodegenerative disease. In recent years, numerous progresses in the discovery of novel Alzheimer's disease molecular biomarkers in brain as well as in biological fluids have been made. Among them, those involving lipid metabolism are emerging as potential candidates. In particular, an accumulation of neutral lipids was recently found by us in skin fibroblasts from Alzheimer's disease patients. Therefore, with the aim to assess whether peripheral alterations in cholesterol homeostasis might be relevant in Alzheimer's disease development and progression, in the present study we analyzed lipid metabolism in plasma and peripheral blood mononuclear cells from Alzheimer's disease patients and from their first-degree relatives., Methods: Blood samples were obtained from 93 patients with probable Alzheimer's disease and from 91 of their first-degree relatives. As controls we utilized 57, cognitively normal, over-65 year-old volunteers and 113 blood donors aged 21-66 years, respectively. Data are reported as mean +/- standard error. Statistical calculations were performed using the statistical analysis software Origin 8.0 version. Data analysis was done using the Student t-test and the Pearson test., Results: Data reported here show high neutral lipid levels and increased ACAT-1 protein in about 85% of peripheral blood mononuclear cells freshly isolated (ex vivo) from patients with probable sporadic Alzheimer's disease compared to about 7% of cognitively normal age-matched controls. A significant reduction in high density lipoprotein-cholesterol levels in plasma from Alzheimer's disease blood samples was also observed. Additionally, correlation analyses reveal a negative correlation between high density lipoprotein-cholesterol and cognitive capacity, as determined by Mini Mental State Examination, as well as between high density lipoprotein-cholesterol and neutral lipid accumulation. We observed great variability in the neutral lipid-peripheral blood mononuclear cells data and in plasma lipid analysis of the subjects enrolled as Alzheimer's disease-first-degree relatives. However, about 30% of them tend to display a peripheral metabolic cholesterol pattern similar to that exhibited by Alzheimer's disease patients., Conclusion: We suggest that neutral lipid-peripheral blood mononuclear cells and plasma high density lipoprotein-cholesterol determinations might be of interest to outline a distinctive metabolic profile applying to both Alzheimer's disease patients and asymptomatic subjects at higher risk of disease.
- Published
- 2009
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37. Rural surgery in southern Sudan.
- Author
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Meo G, Andreone D, De Bonis U, Cometto G, Enrico S, Giustetto G, Kiss A, Landra M, Palmas M, Sacchi L, Taliente P, and Vergnano G
- Subjects
- Anesthesia methods, Cause of Death, Feasibility Studies, Humans, Patient Care Team, Postoperative Complications mortality, Sudan, Surgical Procedures, Operative statistics & numerical data, Developing Countries, Medical Missions, Rural Health, Surgical Procedures, Operative education
- Abstract
Introduction: This article reports a retrospective analysis of a 6-year experience of providing surgical care in remote areas of southern Sudan under extremely adverse conditions., Methods: Teams of expatriate consultants (surgeon, anesthetist, scrub nurse) carried out 28 "surgical missions" with the aims of treating surgical cases previously selected and of training local personnel in basic surgery on the job., Results: A total of 1642 patients (71% males, 30% under the age of 16) have undergone an operation. Altogether, 1264 elective procedures (77%) and 378 emergency procedures (23%) were performed. Hernia surgery comprised the main workload, followed by proctologic and gynecologic operations. Most operations were performed under spinal anesthesia. Other cases required ketamine, and a small number of patients had local anesthesia. There were 14 fatal complications, most of them related to the delay in obtaining medical attention. Based on the training results, the Sudanese personnel of two of the five health centers involved in the program are already fully autonomous. Two doctors and two nurses are proficient in essential surgery; two not qualified nurses are proficient in "primary" anesthesia; and others are proficient in scrubbing and surgical nursing., Conclusions: This report demonstrates that it is feasible to establish surgical services in rural areas of developing countries by utilizing simple facilities, providing them with basic equipment, and employing local personnel selected and trained on the job by teams composed of a consultant surgeon, anesthetist, and scrub nurse. This seems to be the only realistic possibility for providing surgical care to the rural populations of the least developed countries.
- Published
- 2006
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38. Bitter taste study in a sardinian genetic isolate supports the association of phenylthiocarbamide sensitivity to the TAS2R38 bitter receptor gene.
- Author
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Prodi DA, Drayna D, Forabosco P, Palmas MA, Maestrale GB, Piras D, Pirastu M, and Angius A
- Subjects
- Humans, Italy, Receptors, Cell Surface physiology, Receptors, G-Protein-Coupled, Phenylthiourea pharmacology, Receptors, Cell Surface genetics, Taste genetics, Taste physiology
- Abstract
Recently, a major locus on chromosome 7q was found in association with the taste sensitivity to phenylthiocarbamide (PTC) in humans. This region contains the TAS2R38 gene that encodes a member of the TAS2R bitter taste receptor family. Three SNPs within this gene demonstrated a strong association with taster status in Utah families and in an additional sample of 85 unrelated individuals. We studied a small isolated village in eastern Sardinia and carried out a genome-wide scan to map the genetic basis of PTC perception in this population. We performed both qualitative and quantitative PTC-taste linkage analysis. Qualitative analysis was carried out by defining a cut-off from the bimodal distribution of the trait and classifying subjects as tasters and non-tasters (75 and 25%, respectively). Linkage analysis on 131 subjects belonging to a unique large multi-generation pedigree comprising 239 subjects confirmed significant evidence for linkage at 7q35 also in our population. Haplotype analyses of the three SNPs inside the PTC gene allowed us to identify only two haplotypes that were associated with the non-taster phenotype (80% AVI homozygous) and to taster phenotype (40% PAV homozygous and 56% PAV/AVI heterozygous). Sex, age and haplotype effect explained 77.2 % of the total variance in PTC sensitivity., (Copyright 2004 Oxford University Press)
- Published
- 2004
- Full Text
- View/download PDF
39. Family-based association study between bipolar disorder and DRD2, DRD4, DAT, and SERT in Sardinia.
- Author
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Bocchetta A, Piccardi MP, Palmas MA, Chillotti C, Oi A, and Del Zompo M
- Subjects
- Adolescent, Adult, Age of Onset, Alleles, Dopamine Plasma Membrane Transport Proteins, Female, Genotype, Humans, Italy, Linkage Disequilibrium, Male, Parents, Polymorphism, Genetic, Receptors, Dopamine D4, Serotonin Plasma Membrane Transport Proteins, Bipolar Disorder genetics, Carrier Proteins genetics, Membrane Glycoproteins genetics, Membrane Transport Proteins, Nerve Tissue Proteins, Receptors, Dopamine D2 genetics
- Abstract
Association analysis of candidate genes may represent a strategy for clarifying the genetic components involved in bipolar disorder. Polymorphism at dopamine receptor genes DRD2, DRD4, and dopamine and serotonin transporter genes (DAT, SERT) has been used in previous association studies. Some authors have reported positive association between certain alleles and bipolar disorder, using the case-control design. In this family-based association study of DRD2, DRD4, DAT, and SERT, the distribution of parental nontransmitted alleles was compared with that of alleles transmitted to 53 Sardinian probands suffering from bipolar disorder. The transmission disequilibrium test (TDT) was used to detect any disproportionate transmission of alleles by heterozygous parents to affected children. No differences were found between the allele distribution of polymorphisms at DRD2, DRD4, DAT, and SERT in probands and parental nontransmitted chromosomes. TDT did not reveal any difference between transmitted and nontransmitted alleles. Our results do not support the hypothesis of a role for DRD2, DRD4, DAT, or SERT in bipolar disorder. Previously reported positive associations between DRD2 or SERT and bipolar disorder were conceivably due to stratification dependent on the case-control design, even though our sample might have failed to detect small associations due to limited power.
- Published
- 1999
40. The influence of diabetes mellitus on primary open angle glaucoma perimetry.
- Author
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Zucca I, Tanda A, Piras V, Mulas S, Mureddu E, Lecis F, Sulis R, Palmas MC, Fossarello M, and Serra A
- Subjects
- Diabetes Mellitus physiopathology, Disease Progression, Glaucoma, Open-Angle pathology, Glaucoma, Open-Angle physiopathology, Humans, Image Processing, Computer-Assisted, Middle Aged, Retina pathology, Visual Fields, Diabetes Complications, Glaucoma, Open-Angle complications, Visual Field Tests
- Abstract
We have carried out a study into retinal sensitivity alterations in the course of primary open angle glaucoma to see if their appearance and evolution might be influenced by concomitant diabetes mellitus. The visual field examination (Perimeter Octopus 500 EZ, programme G1) indicated prevalent sensitivity defects in the superior hemifield, both in glaucoma only subjects and in those with diabetes as well. As to the inferior hemifield, a greater, statistically significant, retinal sensitivity defect was found in the inferior temporal quadrant of the left eye in the group of diabetics.
- Published
- 1997
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41. The role of laparoscopy in the treatment of endometriosis.
- Author
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Mais V, Ajossa S, Guerriero S, Paoletti AM, Palmas M, Mascia M, and Melis GB
- Subjects
- Endometriosis diagnosis, Female, Humans, Ovarian Cysts diagnosis, Sensitivity and Specificity, Treatment Outcome, Uterine Diseases diagnosis, Endometriosis surgery, Laparoscopy, Uterine Diseases surgery
- Abstract
The aim of the present study was to evaluate the role of laparoscopy not only in the diagnosis but also in the therapy of pelvic endometriosis. Ninety-four patients underwent laparoscopy between May 1991 and May 1993. The patients were divided into 2 groups, according to the indication for laparoscopy: group I (benign ovarian cysts n = 47); group II (chronic pelvic pain, n = 47). All laparoscopies were performed by 2 surgeons only. When present, endometriosis was scored according to the American Fertility Society revised classification 1985 (AFS 1985). Endometriosis was present in 37 (39.4%) of the 94 patients included in the study: 19 out of 47 (40.4%) in group I, 18 out of 47 (38.3%) in group II. In 29 patients with endometriosis (78.3%), the score was reduced surgically during the diagnostic procedure. In 24 cases (82.8%) by laparoscopy and in 5 cases (17.2%) only, by laparotomy. Therefore, the careful selection of cases, the use of appropriate instruments and the experience in endoscopic surgery, combined with a good knowledge of pelvic anatomy, may allow the treatment of endometriosis immediately after diagnosis by laparoscopy, resulting in shorter hospitalization, less physical trauma, and a lower number of post-operative adhesions.
- Published
- 1994
42. Experimental cultivation of cannabis plants in the Mediterranean area.
- Author
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Cortis G, Luchi P, and Palmas M
- Subjects
- Cannabinoids analysis, Chromatography, Gas, Dronabinol analysis, Drug and Narcotic Control, Italy, Seeds, Cannabis analysis
- Abstract
In research carried out in 1982, which included the cultivation of cannabis plants with low, medium and high levels of delta 9-tetrahydrocannabinol (THC), the authors have determined the parameters for individualization and classification of cannabis plants according to their intoxicant potential. This can help to provide courts of law with valid supportive expertise on cannabis trafficking cases. The parameters are the percentages of THC in cannabinoids and in the dried substance of a plant, as well as the percentage of cannabinoids in the dried substance. On the basis of these parameters, the authors have found that a cannabis plant in which the percentage of THC exceeds 50 per cent of the total amount of cannabinoids of the extractable resin and 0.3 per cent of the total amount of dried substance, and in which the amounts of resin and cannabinoids are substantial, has a considerable intoxicant potential and is liable to be used for illicit production of cannabis for abuse. On the contrary, a plant with a THC level below 50 per cent of the cannabinoids and 0.3 per cent of the dried substance, in addition to a low level of total cannabinoids, has low intoxicant potential and can be used in industry for the production of oil and rope. On the basis of these parameters it is also possible to predict the intoxicant potential of a young cannabis plant harvested at a relatively early stage of its development.
- Published
- 1985
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