1. Molecular cloning, characterization and expression of immunoglobulin D on pathogen challenge and pathogen associated molecular patterns stimulation in freshwater carp, Catla catla.
- Author
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Banerjee R, Patel B, Basu M, Lenka SS, Paicha M, Samanta M, and Das S
- Subjects
- Aeromonas hydrophila immunology, Aeromonas hydrophila pathogenicity, Amino Acid Sequence, Animals, Arguloida pathogenicity, Bacterial Infections immunology, Cyprinidae immunology, DNA, Complementary genetics, Fish Diseases microbiology, Fish Diseases parasitology, Fish Diseases virology, Fresh Water, Gene Expression, Gene Expression Profiling veterinary, Gram-Negative Bacterial Infections immunology, Gram-Negative Bacterial Infections veterinary, Host-Parasite Interactions genetics, Host-Parasite Interactions immunology, Host-Pathogen Interactions genetics, Host-Pathogen Interactions immunology, Immunoglobulin D classification, Immunoglobulin Heavy Chains chemistry, Immunoglobulin Heavy Chains genetics, Immunoglobulin Isotypes chemistry, Immunoglobulin Isotypes genetics, Kidney, Parasitic Diseases immunology, Phylogeny, Rhabdoviridae pathogenicity, Sequence Analysis, Protein, Skin immunology, Streptococcal Infections immunology, Streptococcal Infections veterinary, Streptococcus pathogenicity, Virus Diseases immunology, Carps genetics, Carps immunology, Cloning, Molecular, Fish Diseases immunology, Gene Expression Regulation, Immunoglobulin D chemistry, Immunoglobulin D genetics, Pathogen-Associated Molecular Pattern Molecules
- Abstract
The primordial immunoglobulin class, IgD, was the first non-IgM isotype discovered in teleosts. The crucial roles of IgM and IgZ in imparting systemic and mucosal immunity, respectively, in various fish species have been widely established. However, the putative function of a unique IgD isotype during pathogenic invasions has not been well explored. The present study reports the existence of an IgD ortholog in freshwater carp, Catla catla, and further evaluates its differential expression profile in response to bacterial, parasitic and viral antigenic exposure and pathogen associated molecular patterns (PAMPs) stimulation. The IgD of C. catla (CcIgD) cDNA sequence was found to encode 226 amino acids and confirmed homology with heavy chain delta region of Cyprinidae family members. Phylogenetic analysis of CcIgD exhibited greatest similarity with Ctenopharyngodon idella. qRT-PCR analysis revealed significant upregulation (P < 0.001) of IgD gene expression in kidney with respect to other tissues at 24 hr post-Aeromonas hydrophila challenge. CcIgD gene expression in skin was enhanced following Streptococcus uberis infection and in blood following Argulus infection and inactivated rhabdoviral antigen stimulation. Further, the treatment of bacterial and viral products (PAMPs) also triggered significant (P < 0.05) increases in CcIgD mRNA expression in kidney. These findings indicate the functional importance of teleost IgD in orchestrating tissue specific neutralization of antigens on stimulation with different pathogens and PAMPs., (© 2017 The Societies and John Wiley & Sons Australia, Ltd.)
- Published
- 2017
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