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179 results on '"Paglietti, Bianca"'

3. Enterobacterales carrying chromosomal AmpC β-lactamases in Europe (EuESCPM): Epidemiology and antimicrobial resistance burden from a cohort of 27 hospitals, 2020–2022

Author

Boattini, Matteo, Bianco, Gabriele, Llorente, Laura Iglesias, Acero, Laura Alonso, Nunes, Daniel, Seruca, Miguel, Mendes, Vasco Santos, Almeida, André, Bastos, Paulo, Rodríguez-Villodres, Ángel, Gascón, Adelina Gimeno, Halperin, Ana Verónica, Cantón, Rafael, Escartín, Maria Nieves Larrosa, González-López, Juan José, Floch, Pauline, Massip, Clémence, Chainier, Delphine, Barraud, Olivier, Dortet, Laurent, Cuzon, Gaëlle, Zancanaro, Clément, Mizrahi, Assaf, Schade, Rogier, Rasmussen, Asger Nellemann, Schønning, Kristian, Hamprecht, Axel, Schaffarczyk, Lukas, Glöckner, Stefan, Rödel, Jürgen, Kristóf, Katalin, Balonyi, Ágnes, Mancini, Stefano, Quiblier, Chantal, Fasciana, Teresa, Giammanco, Anna, Paglietti, Bianca, Rubino, Salvatore, Budimir, Ana, Bedenić, Branka, Rubic, Zana, Marinović, Jelena, Gartzonika, Konstantina, Christaki, Eirini, Mavromanolaki, Viktoria Eirini, Maraki, Sofia, Yalçın, Tuğba Yanık, Azap, Özlem Kurt, Licker, Monica, Musuroi, Corina, Talapan, Daniela, Vrancianu, Corneliu Ovidiu, Comini, Sara, Zalas-Więcek, Patrycja, Michalska, Anna, Cavallo, Rossana, Melo Cristino, José, and Costa, Cristina

Published
2024
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18. High-risk lineages among extended-spectrum β-lactamase-producing Escherichia coli from extraintestinal infections in Maputo Central Hospital, Mozambique

Author

Santona, Antonella, primary, Sumbana, José João, additional, Fiamma, Maura, additional, Deligios, Massimo, additional, Taviani, Elisa, additional, Simbine, Samuel Elija, additional, Zimba, Tomas, additional, Sacarlal, Jahit, additional, Rubino, Salvatore, additional, and Paglietti, Bianca, additional

Published
2022
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20. Characteristics of Antibiotic-resistant E. coli Isolated from Perfume River in Hue City, Vietnam.

Author

Ngo Viet Quynh Tram, Phung Thi Thu Thuy, Nguyen Thi Dang Khoa, Nguyen Thi Tuyen, Pham Thi Hong Cam, Ngo Thi Dieu Huong, Ton That Canh Tri, Nguyen Hoang Bach, and Paglietti, Bianca

Abstract

Antibiotic resistant bacteria (ARB) in aquatic environments is attracting increasing attention. However, the spread of ARB along Perfume River in Hue City is poorly understood. This study aimed to phenotypically and genotypically characterize β-lactam- and fluoroquinolone-resistant Escherichia coli isolates from this river. Water samples were collected from the urban, rural, agricultural, and less-affected areas in March 2020. E. coli susceptibility to seven commonly employed antibiotics was analyzed using the disk diffusion method, and the antibiotic resistance genes (ARGs), qnrA, qnrB, qnrS, TEM, SHV, and CTX-M, were identified using polymerase chain reaction (PCR) and DNA sequencing. The antibiotic susceptibility patterns of E. coli revealed that the rate of amoxicillin resistance was the highest (60%). PCR assays and sequencing of 12 β-lactam-resistant E. coli isolates indicated the presence of blaTEM and blaCTX-M-15 in 58.3% and 16.7% of the isolates, respectively. Only one of four fluoroquinolon -resistant E. coli isolates harbored the qnrS, while qnrA or qnrB genes were not detected. These findings suggest that this water may be an essential source of transmissible ARGs in Hue City, which may have a detrimental impact on the people living in this area. [ABSTRACT FROM AUTHOR]

Published
2023
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23. Occult Vancomycin-Resistant Enterococcus faecium ST117 Displaying a Highly Mutated vanB 2 Operon.

Author

Santona, Antonella, Taviani, Elisa, Fiamma, Maura, Deligios, Massimo, Hoang, Hoa M., Sanna, Silvana, Rubino, Salvatore, and Paglietti, Bianca

Subjects
ENTEROCOCCUS, ENTEROCOCCUS faecium, ENTEROCOCCAL infections, MISSENSE mutation, OCCULTISM, VANCOMYCIN resistance, GENOTYPES
Abstract

Rare information is available on clinical Enterococcus faecium encountered in Sardinia, Italy. This study investigated the antimicrobial susceptibility profiles and genotypic characteristics of E. faecium isolated at the University Hospital of Sassari, Italy, using the Vitek2 system and PCR, MLST, or WGS. Vitek2 revealed two VanB-type vancomycin-resistant Enterococcus faecium (VREfm) isolates (MICs mg/L = 8 and ≥32) but failed to detect vancomycin resistance in one isolate (MIC mg/L ≤ 1) despite positive genotypic confirmation of vanB gene, which proved to be vancomycin resistant by additional phenotypic methods (MICs mg/L = 8). This vanB isolate was able to increase its vancomycin MIC after exposure to vancomycin, unlike the "classic" occult vanB-carrying E. faecium, becoming detectable by Vitek 2 (MICs mg/L ≥ 32). All three E. faecium had highly mutated vanB2 operons, as part of a chromosomally integrated Tn1549 transposon, with common missense mutations in VanH and VanB2 resistance proteins and specific missense mutations in the VanW accessory protein. There were additional missense mutations in VanS, VanH, and VanB proteins in the vanB2-carrying VREfm isolates compared to Vitek2. The molecular typing revealed a polyclonal hospital-associated E. faecium population from Clade A1, and that vanB2-VREfm, and nearly half of vancomycin-susceptible E. faecium (VSEfm) analyzed, belonged to ST117. Based on core genome-MLST, ST117 strains had different clonal types (CT), excluding nosocomial transmission of specific CT. Detecting vanB2-carrying VREfm isolates by Vitek2 may be problematic, and alternative methods are needed to prevent therapeutic failure and spread. [ABSTRACT FROM AUTHOR]

Published
2023
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25. “Swab Team” in the SARS-CoV-2 outbreak containment among healthcare workers

Author

Bussu, Francesco, primary, Rizzo, Davide, additional, Saderi, Laura, additional, Piras, Antonio, additional, De Luca, Laura Maria, additional, De Riu, Giacomo, additional, Vaira, Luigi Angelo, additional, Serra, Antonello, additional, Altrudo, Paola, additional, Delogu, Domenico, additional, Dettori, Ignazio, additional, Riu, Francesco Giuseppe, additional, Angioj, Flavia, additional, Firino, Laura, additional, Govoni, Rosalba, additional, Ibba, Gabriele, additional, Lai, Vincenzo, additional, Mura, Erica, additional, Paglietti, Bianca, additional, Piu, Claudia, additional, Puggioni, Anna, additional, Rimini, Elena, additional, Rocca, Giulia, additional, Serra, Caterina, additional, Uzzau, Sergio, additional, Rubino, Salvatore, additional, and Sotgiu, Giovanni, additional

Published
2021
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26. Evolution and population structure of Salmonella enterica serovar Newport

Author

Sangal, Vartul, Harbottle, Heather, Mazzoni, Camila J., Helmuth, Reiner, Guerra, Beatriz, Didelot, Xavier, Paglietti, Bianca, Rabsch, Wolfgang, Brisse, Sylvain, Weill, Francois-Xavier, Roumagnac, Philippe, and Achtman, Mark

Subjects
Salmonella -- Genetic aspects, Salmonella -- Physiological aspects, Evolution -- Research, Biological sciences
Abstract

Salmonellosis caused by Salmonella enterica serovar Newport is a major global public health concern, particularly because S. Newport isolates that are resistant to multiple drugs (MDR), including third-generation cephalosporins (MDR-AmpC phenotype), have been commonly isolated from food animals. We analyzed 384 S. Newport isolates from various sources by a multilocus sequence typing (MLST) scheme to study the evolution and population structure of the serovar. These were compared to the population structure of S. enterica serovars Enteritidis, Kentucky, Paratyphi B, and Typhimurium. Our S. Newport collection fell into three lineages, Newport-I, Newport-II, and Newport-III, each of which contained multiple sequence types (STs). Newport-I has only a few STs, unlike Newport-II or Newport-III, and has possibly emerged recently. Newport-I is more prevalent among humans in Europe than in North America, whereas Newport-II is preferentially associated with animals. Two STs of Newport-II encompassed all MDR-AmpC isolates, suggesting recent global spread after the acquisition of the [bla.sub.CMY-2] gene. In contrast, most Newport-III isolates were from humans in North America and were pansusceptible to antibiotics. Newport was intermediate in population structure to the other serovars, which varied from a single monophyletic lineage in S. Enteritidis or S. Typhimurium to four discrete lineages within S. Paratyphi B. Both mutation and homologous recombination are responsible for diversification within each of these lineages, but the relative frequencies differed with the lineage. We conclude that serovars of S. enterica provide a variety of different population structures. doi: 10.1128/JB.00969-10

Published
2010

27. An exceptional case report of disseminated cryptococcosis in a hitherto immunocompetent patient

Author

Adzic-Vukicevic, Tatjana, Cevik, Muge, Poluga, Jasmina, Micic, Jelena, Rubino, Salvatore, Paglietti, Bianca, and Barac, Aleksandra

Subjects
Cryptococcus, Prostatic adenocarcinoma, Disseminated infection, Malignancy
Abstract

Cryptococcosis is an opportunistic fungal infection causes significant disease predominantly in immunocompromised patients. Here we present an excepcional case of disseminated cryptococcosis with pulmonary and cerebral involvement in an immunocompetent patient with no apparent predisposing factors at the time of hospital admission. We described a case of an apparently immunocompetent 66-years old man admitted to hospital with a one-month history of cough, fever and vertigo. During hospitalization, thorax imaging was suggestive of lung metastasis, therefore, he went through several investigations. During hospitalization, he developed neurological symptoms and subsequently underwent a lumbar puncture. Cerebrospinal fluid (CSF) culture was positive for Cryptococcus spp. isolated on Sabouraud’s dextrose agar and bird seed agar. In addition, the direct microscopy examination was positive for the India ink test, as well as with the latex agglutination test for cryptococcal polysaccharide antigen (CrAg) in CSF, while serum CrAg was negative. Despite the absence of classic immunocompromising features, he was treated with amphotericin B and fluconazole due to suspected disseminated cryptococcal infection. Later, he was diagnosed with prostatic adenocarcinoma. Upon successful completion of treatment for disseminated cryptococcosis, the patient underwent radical prostate ablation surgery as a treatment forprostatic adenocarcinoma. This exceptional case emphasizes the high degree of suspicion of atypical infections, and in these cases, it is particularly important to consider fungal infections in hitherto healthy patients with no apparent predisposing factors. Although Cryptococcus spp. is predominantly reported in patients with hematological malignancies, cryptococcosis investigation should also be considered as part of the initial workup of patients with a new diagnosis of a solid tumour prior to chemotherapy or radiotherapy.

Published
2020

30. Short epidemiological overview of the current situation on COVID-19 pandemic in Southeast European (SEE) countries

Author

Puca, Edmond, primary, Čivljak, Rok, additional, Arapović, Jurica, additional, Popescu, Corneliu, additional, Christova, Iva, additional, Raka, Lul, additional, Cana, Fadil, additional, Miranović, Vesna, additional, Karageorgopoulos, Drosos, additional, Baš, Denis, additional, Paglietti, Bianca, additional, and Barać, Aleksandra, additional

Published
2020
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36. Cover Image

Author

Murgia, Manuela, primary, Fiamma, Maura, additional, Barac, Aleksandra, additional, Deligios, Massimo, additional, Mazzarello, Vittorio, additional, Paglietti, Bianca, additional, Cappuccinelli, Pietro, additional, Al‐Qahtani, Ahmed, additional, Squartini, Andrea, additional, Rubino, Salvatore, additional, and Al‐Ahdal, Mohammed N., additional

Published
2019
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37. Polyclonal emergence of MDR Enterobacter cloacaecomplex isolates producing multiple extended spectrum beta-lactamases at Maputo Central Hospital, Mozambique

Author

Sumbana, José, Santona, Antonella, Fiamma, Maura, Taviani, Elisa, Deligios, Massimo, Chongo, Ventura, Sacarlal, Jahit, Rubino, Salvatore, and Paglietti, Bianca

Abstract

Enterobacterspp. are important nosocomial pathogens responsible of a wide variety of infections, mainly due to Extended Spectrum β-Lactamase (ESBL) producing isolates, constituting a global public health issue in terms of clinical treatment and infection control, especially in low-income countries, where last-line treatment is often unavailable and there is weak nosocomial surveillance. In this study, we conducted a phenotypic and molecular characterization of 8 clinical Enterobacterspp. strains, isolated from patient’s blood in three hospitals in Mozambique. Isolates were identified by MALDI-TOF and antimicrobial Susceptibility Testing was performed by VITEK 2 system. Half of isolates were analyzed by PCR for β-lactamases genes, other isolates by Whole Genome Sequencing. We identified all isolates as Enterobacter cloacaecomplex (ECC), those from Maputo Central Hospital were polyclonal, multidrug resistant (5/8), and ESBL producers (50%), carrying blaCTX-M-15and different assortment of blaSHV-12, blaTEM-1Band blaOXA-1, and AmpCs blaCMH-3, blaACT-7and blaACT-9genes. Resistance determinants linked to fluoroquinolone (aac(6')Ib-crand qnrB1) and others antimicrobials were also found. Notably, one isolate showed phenotypically resistance to colistin, while another colistin susceptible isolate carried a silent mcr-9gene. ECC nosocomial surveillance is urgently needed to contain and prevent the dissemination of ESBLs producing clones, and mcr-9spread to other Enterobacteriaceae.

Published
2022
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39. Biodiversity of fungi in hot desert sands

Author

Murgia, Manuela, primary, Fiamma, Maura, additional, Barac, Aleksandra, additional, Deligios, Massimo, additional, Mazzarello, Vittorio, additional, Paglietti, Bianca, additional, Cappuccinelli, Pietro, additional, Al-Qahtani, Ahmed, additional, Squartini, Andrea, additional, Rubino, Salvatore, additional, and Al-Ahdal, Mohammed N., additional

Published
2018
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46. Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan

Author

Khan, Adnan, Farooqui, Amber, Raza, Yasir, Rasheed, Faisal, Manzoor, Hamid, Akhtar, Syed Shakeel, Quraishy, Muhammad Saeed, Rubino, Salvatore, Kazmi, Shahana U., and Paglietti, Bianca

Subjects
bacterial infections and mycoses, digestive system diseases, MED/07 Microbiologia e microbiologia clinica
Abstract

Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes and their association with different clinical outcomes among dyspeptic patients in Pakistan over a period of four years. Methodology: Gastric biopsy samples from a total of 450 dyspeptic individualswere subjected to PCR, genotypingand histology. Results: A total of 201 (45%) cases were found positive for H. pylori. The detection rate was high in GU (91%), DU (86%) and GC (83%) cases compared with those cases who had intact gastric mucosa (18%). Histology revealed the presence of infection in 68% of cases of mild/chronic nonspecific gastritis with others belonging to the GU sequel. cagA gene carriage was observed in 104 (51%) cases or mostly from DU, GU and GC groups, of which 97 were Western type strains while 3 were East-Asian type strains that are rarely observed in South Asia. vacA allelic variant s1am1 was most commonly observed, followed by s1am2, and s1bm1, with direct correlation in diseased cases (gastritis, GU, DU and GC). Prevalent genotypic combinations were s1am1/cagA- in gastritis and s1am1/cagA+ in DU, GU, and GC. Conclusions: Our study indicates the predominant circulation of Western type cagA and vacAs1am1 type H. pylori strains in Pakistan.

Published
2013

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