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235 results on '"Padula, Cristina"'

9. Nanostructured Lipid Carriers for Enhanced Transscleral Delivery of Dexamethasone Acetate: Development, Ex Vivo Characterization and Multiphoton Microscopy Studies

Author

González-Fernández, Felipe M, Delledonne, Andrea, Nicoli, Sara, Gasco, Paolo, Padula, Cristina, Santi, Patrizia, Sissa, Cristina, and Pescina, Silvia

Subjects
ocular delivery, tyloxapol, multiphoton microscopy, ex vivo, NLC, Pharmaceutical Science, dexamethasone, posterior segment, dexamethasone acetate
Abstract

Corticosteroids, although highly effective for the treatment of both anterior and posterior ocular segment inflammation, still nowadays struggle for effective drug delivery due to their poor solubilization capabilities in water. This research work aims to develop nanostructured lipid carriers (NLC) intended for periocular administration of dexamethasone acetate to the posterior segment of the eye. Pre-formulation studies were initially performed to find solid and liquid lipid mixtures for dexamethasone acetate solubilization. Pseudoternary diagrams at 65 °C were constructed to select the best surfactant based on the macroscopic transparency and microscopic isotropy of the systems. The resulting NLC, obtained following an organic solvent-free methodology, was composed of triacetin, Imwitor® 491 (glycerol monostearate >90%) and tyloxapol with Z-average = 106.9 ± 1.2 nm, PDI = 0.104 ± 0.019 and zeta potential = −6.51 ± 0.575 mV. Ex vivo porcine sclera and choroid permeation studies revealed a considerable metabolism in the sclera of dexamethasone acetate into free dexamethasone, which demonstrated higher permeation capabilities across both tissues. In addition, the NLC behavior once applied onto the sclera was further studied by means of multiphoton microscopy by loading the NLC with the fluorescent probe Nile red.

Published
2023

13. An Alternative Device for the Topical Treatment of Oral Cancer: Development and Ex-Vivo Evaluation of Imiquimod-Loaded Polysaccharides Formulations

Author

Remiro, Paula de Freitas Rosa, primary, Nagahara, Mariana Harue Taniguchi, additional, Ghezzi, Martina, additional, Filippini, Alessia, additional, Demurtas, Anna, additional, Pescina, Silvia, additional, Santi, Patrizia, additional, Padula, Cristina, additional, Moraes, Ângela Maria, additional, and Nicoli, Sara, additional

Published
2022
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16. Buccal Permeation of Polysaccharide High Molecular Weight Compounds: Effect of Chemical Permeation Enhancers.

Author

Fantini, Adriana, Giulio, Luca, Delledonne, Andrea, Pescina, Silvia, Sissa, Cristina, Nicoli, Sara, Santi, Patrizia, and Padula, Cristina

Subjects
MOLECULES, MOLECULAR weights, POLYSACCHARIDES, DEXTRAN, BUCCAL administration, OCTANOIC acid
Abstract

The greatest achievement in the advanced drug delivery field should be the optimization of non-invasive formulations for the delivery of high molecular weight compounds. Peptides, proteins, and other macromolecules can have poor membrane permeation, principally due to their large molecular weight. The aim of this work was to explore the possibility of administering fluorescently labeled dextrans (molecular weight 4–150 kDa) across the buccal mucosa. Permeation experiments across pig esophageal mucosa were carried out using fatty acids and bile salts as penetration enhancers. The data obtained show that it is possible to increase or promote the mucosa permeation of high molecular weight dextrans by using caprylic acid or sodium taurocholate as the chemical enhancers. With these enhancers, dextrans with molecular weight of 70 and 150 kDa, that in passive conditions did not permeate, could cross the mucosa in detectable amounts. FD-70 and FD-150 showed comparable permeability values, despite the molecular weight difference. The results obtained in the present work suggest that the buccal administration of high molecular weight compounds is feasible. [ABSTRACT FROM AUTHOR]

Published
2023
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23. Topical Drug Delivery: Innovative Controlled Release Systems.

Author

de Araujo, Daniele Ribeiro and Padula, Cristina

Subjects
*TOPICAL drug administration, *DRUG solubility, *POLOXAMERS, *LIPOSOMES, *DRUGS, *LIQUID crystal states
Abstract

Structural organization, skin delivery performance and permeation mechanisms were assessed, aiming to find different anti-cancer, hormone replacement and skin inflammatory lesions therapies. One of the most innovative strategies for administrating bioactive molecules is the design of adequate drug delivery systems. In addition to drug permeation, authors also described innovative strategies to improve drugs skin retention and reducing at the same time transdermal flux. [Extracted from the article]

Published
2023
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36. Determination of Ganciclovir in Plasma of Newborns with Congenital CMV Infection

Author

Padula Cristina, Santi Patrizia, Buffolano Wilma, Laneri Sonia, Dini Irene, Laneri, S., Padula, C., Buffolano, W., Santi, P., and Dini, I.

Subjects
Ganciclovir, Fetus, HPLC-UV, Transmission (medicine), business.industry, Congenital cytomegalovirus infection, virus diseases, Transplacental, Viremia, Analytic Method, General Medicine, medicine.disease, 030226 pharmacology & pharmacy, Virology, Congenital cmv infection, 03 medical and health sciences, 0302 clinical medicine, medicine, 030212 general & internal medicine, business, Subclinical infection, medicine.drug
Abstract

Infections caused by human cytomegalovirus are important causes of fetal and neonatal morbidity and mortality. Ganciclovir ([(9-(1,3-dihydroxy-2- propoxymethyl) guanine, GCV) is a synthetic acyclic nucleoside which has shown activity against Cytomegalovirus. GCV treatment has been associated with serious toxic hematological effects such as neutropenia and leukopenia, thus drug monitoring is needed, especially in the case of newborns. The aim of the work is to develop and validate an HPLC method for the quantification of GCV in plasma

Published
2019

37. Ucuùba (Virola surinamensis) Fat-Based Nanostructured Lipid Carriers for Nail Drug Delivery of Ketoconazole: Development and Optimization Using Box-Behnken Design

Author

Pereira, Rayanne R., Testi, Matteo, Rossi, Francesca, Silva Junior, Jose O. C., Ribeiro-Costa, Roseane M., Bettini, Ruggero, Santi, Patrizia, Padula, Cristina, and Sonvico, Fabio

Subjects
nanotechnology
Abstract

Ucuùba fat is fat obtained from a plant found in South America, mainly in Amazonian Brazil. Due to its biocompatibility and bioactivity, the Ucuùba fat was used for production of ketoconazole-loaded nanostructured lipid carriers (NLC) in view of an application for the treatment of onychomycosis and other persistent fungal infections. The development and optimization of the Ucuùba fat based NLC were performed using a Box-Behnken design of experiment. The independent variables were surfactant concentration (% w/v), liquid lipids concentration (% w/v), solid lipids concentration (% w/v), while the outputs of interest were particle size, polydispersity index (PDI) and drug encapsulation efficiency (EE). The Ucuùba fat based NLC were produced and the process optimized determining a predictive mathematical model. Applying the model, two formulations with the pre-required particle size, i.e., 30 and 85 nm, were produced for further evaluation. The optimized formulations were characterized and showed a particle size in agreement to the predicted value, i.e. 33.6 nm and 74.6 nm, respectively. The optimized formulations were also characterized using multiple techniques in order to investigate the solid state of drug and excipients (DSC and XRD), particle morphology (TEM) and interactions between the formulation components (FTIR). Furthermore, particle size and surface charge of the formulations was studied during a one-month stability study and did not evidence any significative modification during storage.

Published
2019

41. Ucuùba (Virola surinamensis) Fat-Based Nanostructured Lipid Carriers for Nail Drug Delivery of Ketoconazole: Development and Optimization Using Box-Behnken Design

Author

Pereira, Rayanne R., primary, Testi, Matteo, additional, Rossi, Francesca, additional, Silva Junior, Jose O. C., additional, Ribeiro-Costa, Roseane M., additional, Bettini, Ruggero, additional, Santi, Patrizia, additional, Padula, Cristina, additional, and Sonvico, Fabio, additional

Published
2019
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48. Gel-like TPGS-Based Microemulsions for Imiquimod Dermal Delivery: Role of Mesostructure on the Uptake and Distribution into the Skin

Author

Telò, Isabella, Favero, Elena Del, Cantù, Laura, Frattini, Noemi, Pescina, Silvia, Padula, Cristina, Santi, Patrizia, Sonvico, Fabio, and Nicoli, Sara

Abstract

The aim of this work was to develop an innovative microemulsion with gel-like properties for the cutaneous delivery of imiquimod, an immunostimulant drug employed for the treatment of cutaneous infections and neoplastic conditions. A pseudoternary phase diagram was built using a 1/1 TPGS (d-α-tocopheryl polyethylene glycol 1000 succinate)/Transcutol mixture as surfactant system, and oleic acid as oil phase. Eight microemulsionsselected from the 1.25/8.75 oil/surfactants ratio, along the water dilution line (from 20 to 56% w/w)were characterized in terms of rheological behavior, optical properties via polarized microscopy, and supramolecular structure using X-ray scattering. Then, these formulations were loaded with imiquimod and the uptake and distribution into the skin was evaluated on full-thickness porcine skin. X-ray scattering experiments revealed the presence of disconnected drops in the case of microemulsion with 20% water content. Diluting the system up to 48% water content, the structure turned into an interconnected lamellar microemulsion, reaching a proper disconnected lamellar structure for the highest water percentages (52–56%). Upon water addition, also the rheological properties changed from nearly Newtonian fluids to gel-like structures, displaying the maximum of viscosity for the 48% water content. Skin uptake experiments demonstrated that formulation viscosity, drug loading, and surfactant concentration did not play an important role on imiquimod uptake into the skin, while the skin penetration was related instead to the microemulsion mesostructure. In fact, drug uptake became enhanced by locally lamellar interconnected structures, while it was reduced in the presence of disconnected structures, either drops or proper lamellae. Finally, the data demonstrated that mesostructure also affects the drug distribution between the epidermis and dermis. In particular, a significantly higher dermal accumulation was found when disconnected lamellar structures are present, suggesting the possibility of tuning both drug delivery and localization into the skin by modifying microemulsions composition.

Published
2024
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