Search

Your search keyword '"Pacheco, Pedro J."' showing total 26 results
Start Over Author "Pacheco, Pedro J."
26 results on '"Pacheco, Pedro J."'

4. Ensifer meliloti denitrification is involved in infection effectiveness and N2O emissions from alfalfa root nodules

Author

European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Pacheco, Pedro J., Bedmar, Eulogio J., Mesa, Socorro, Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Pacheco, Pedro J., Bedmar, Eulogio J., Mesa, Socorro, Tortosa Muñoz, Germán, and Delgado Igeño, María Jesús

Abstract

Purpose: Alfalfa is one of the most valuable forage crops in temperate climate zones. Ensifer meliloti, the endosymbiont of alfalfa, contains all the denitrification genes but the capacity of alfalfa root nodules to produce nitrous oxide (NO) is not known. In this work, NO emissions as well as the influence of bacteroidal denitrification on nodulation competitiveness and NO release from alfalfa nodules has been investigated. Methods: Medicago sativa cv. Victoria plants were inoculated with E. meliloti 1021, a periplasmic nitrate reductase (Nap) defective mutant, a Nap overexpressing strain and a nitrous oxide reductase defective mutant. Plants were grown in the presence of different nitrate and copper treatments and subjected to flooding during one week before harvesting. NO production by the nodules was analysed by using gas chromatography. Methyl viologen-dependent nitrate reductase (MV-NR), nitrite reductase (MV-NIR) and nitrous oxide reductase (NOR) enzymatic activities were measured in isolated bacteroids. Results: Alfalfa root nodules produce NO in response to nitrate and flooding. Overexpression of Nap improved nodulation competitiveness and induced NO emissions from nodules. Copper is required for an effective symbiosis as well as triggered a reduction of NO production due to the induction of the NOR and a reduction of NIR activities in the bacteroids. Conclusion: Alfalfa root nodules emit NO. Nap is involved in nodulation competitiveness and in NO emissions by the nodules. Bacteroidal NOR and NIR activities are modulated by Cu and may be considered as effective targets for the mitigation strategies of NO emissions derived from alfalfa crops.

Published
2023

5. Pathways and regulatory factors involved in NO and N2O emissions by nitrogen-fixing endosymbiotic bacteria

Author

Delgado Igeño, María Jesús, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Tortosa Muñoz, Germán, Hidalgo-García, Alba, Pacheco, Pedro J., Juárez, Raquel A., Bedmar, Eulogio J., Mesa, Socorro, Delgado Igeño, María Jesús, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Tortosa Muñoz, Germán, Hidalgo-García, Alba, Pacheco, Pedro J., Juárez, Raquel A., Bedmar, Eulogio J., and Mesa, Socorro

Published
2023

6. Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules

Author

European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Hidalgo-García, Alba, Tortosa Muñoz, Germán, Pacheco, Pedro J., Gates, A.J., Richardson, D.J., Bedmar, Eulogio J., Girard, Lourdes, Torres Porras, María Jesús, Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Hidalgo-García, Alba, Tortosa Muñoz, Germán, Pacheco, Pedro J., Gates, A.J., Richardson, D.J., Bedmar, Eulogio J., Girard, Lourdes, Torres Porras, María Jesús, and Delgado Igeño, María Jesús

Abstract

Legumes can contribute to emissions of the potent greenhouse gas nitrous oxide (NO) directly by some rhizobia species that are able to denitrify under free-living conditions and in symbiotic association with the plant. In this study, the capacity of Phaseolus vulgaris-Rhizobium etli symbiosis to emit NO in response to nitrate (NO) has been demonstrated for the first time. We found that bacteroidal assimilatory nitrate reductase (NarB) as well as nitrite reductase (NirK) and nitric oxide reductase (cNor) denitrifying enzymes contribute to nitric oxide (NO) and NO formation in nodules. We also show that R. etli NarK is involved in NO extrusion and links NO reduction by NarB in the cytoplasm with NirK and cNor denitrification activities in the periplasm. The knowledge generated in this work will be instrumental for exploring strategies and sustainable practices in agricultural soil management to increase legume crop yield and mitigate greenhouse gas emissions.

Published
2023

7. The copper-responsive regulator CsoR is indirectly involved in Bradyrhizobium diazoefficiens denitrification

Author

European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Ministerio de Universidades (España), Pacheco, Pedro J., Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Torres Porras, María Jesús, Gates, A.J., Bedmar, Eulogio J., Richardson, D.J., Mesa, Socorro, Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Ministerio de Universidades (España), Pacheco, Pedro J., Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Torres Porras, María Jesús, Gates, A.J., Bedmar, Eulogio J., Richardson, D.J., Mesa, Socorro, Tortosa Muñoz, Germán, and Delgado Igeño, María Jesús

Abstract

The soybean endosymbiont Bradyrhizobium diazoefficiens harbours the complete denitrification pathway that is catalysed by a periplasmic nitrate reductase (Nap), a copper (Cu)-containing nitrite reductase (NirK), a c-type nitric oxide reductase (cNor), and a nitrous oxide reductase (Nos), encoded by the napEDABC, nirK, norCBQD, and nosRZDFYLX genes, respectively. Induction of denitrification genes requires low oxygen and nitric oxide, both signals integrated into a complex regulatory network comprised by two interconnected cascades, FixLJ-FixK2-NnrR and RegSR-NifA. Copper is a cofactor of NirK and Nos, but it has also a role in denitrification gene expression and protein synthesis. In fact, Cu limitation triggers a substantial down-regulation of nirK, norCBQD, and nosRZDFYLX gene expression under denitrifying conditions. Bradyrhizobium diazoefficiens genome possesses a gene predicted to encode a Cu-responsive repressor of the CsoR family, which is located adjacent to copA, a gene encoding a putative Cu+-ATPase transporter. To investigate the role of CsoR in the control of denitrification gene expression in response to Cu, a csoR deletion mutant was constructed in this work. Mutation of csoR did not affect the capacity of B. diazoefficiens to grow under denitrifying conditions. However, by using qRT-PCR analyses, we showed that nirK and norCBQD expression was much lower in the csoR mutant compared to wild-type levels under Cu-limiting denitrifying conditions. On the contrary, copA expression was significantly increased in the csoR mutant. The results obtained suggest that CsoR acts as a repressor of copA. Under Cu limitation, CsoR has also an indirect role in the expression of nirK and norCBQD genes.

Published
2023

9. Novel enzymes involved in N2O emission by Rhizobium etli - common bean symbiosis

Author

Hidalgo-García, Alba, Torres Porras, María Jesús, Tortosa Muñoz, Germán, Pacheco, Pedro J., Gates, Andrew J., Bedmar, Eulogio J., Girard, L., Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), and Junta de Andalucía

Abstract

Resumen del poster presentado en: I Spanish-Portuguese Congress on Beneficial Plant-Microbe Interactions (BeMiPlant) and XVIII National Meeting of the Spanish Society of Nitrogen Fixation (XVIII SEFIN). Oeiras, Portugal, 17-19 octubre (2022), This work was supported by Fondo Europeo de Desarrollo Regional (FEDER)-co-financed grants AGL2017-85676-R from the Ministerio de Ciencia e Innovación (Spain) and grant P18-RT-1401 from the Junta de Andalucía.

Published
2022

10. Control of nitrous oxide emissions by legume-associated endosymbiotic bacteria

Author

Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Pacheco, Pedro J., Juárez, R.A., Bedmar, Eulogio J., Mesa, Socorro, Delgado Igeño, María Jesús, Ministerio de Ciencia e Innovación (España), European Commission, and Junta de Andalucía

Abstract

Conferencia invitada presentada en: 25th Nitrogen Cycle Meeting. Roma 28-20 septiembre (2022), This research has been funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”, AGL2017-85676-R, and PID2020-114330GB-100 grants and also by Junta de Andalucía P18-RT1401 grant. PJP was supported by a FPU fellowship from MCIU (formerly MECD).

Published
2022

11. Análisis transcriptómico de la respuesta de Bradyrhizobium diazoefficiens a la limitación de cobre en condiciones desnitrificantes

Author

Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Pacheco, Pedro J., Juárez, R.A., Bedmar, Eulogio J., Mesa, Socorro, Delgado Igeño, María Jesús, Ministerio de Economía y Competitividad (España), European Commission, and Junta de Andalucía

Abstract

Resumen del poster presentado en: XIII Reunión del Grupo de Microbiología Molecular de La SEM. Granada, 7-9 septiembre (2022), Se agradece el apoyo económico de los proyectos AGL2017-85676-R y PID2020-114330GB-100 (cofinanciados por el Ministerio de Economía y Competitividad y FEDER) y el proyecto P18-RT-1401 (Junta de Andalucía).

Published
2022

13. Novel enzymes involved in N2O emission by Rhizobium etli - common bean symbiosis

Author

European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Hidalgo-García, Alba, Torres Porras, María Jesús, Tortosa Muñoz, Germán, Pacheco, Pedro J., Gates, Andrew J., Bedmar, Eulogio J., Girard, Lourdes, Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Hidalgo-García, Alba, Torres Porras, María Jesús, Tortosa Muñoz, Germán, Pacheco, Pedro J., Gates, Andrew J., Bedmar, Eulogio J., Girard, Lourdes, and Delgado Igeño, María Jesús

Published
2022

14. Control of nitrous oxide emissions by legume-associated endosymbiotic bacteria

Author

Ministerio de Ciencia e Innovación (España), European Commission, Junta de Andalucía, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Pacheco, Pedro J., Juárez, R.A., Bedmar, Eulogio J., Mesa, Socorro, Delgado Igeño, María Jesús, Ministerio de Ciencia e Innovación (España), European Commission, Junta de Andalucía, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Pacheco, Pedro J., Juárez, R.A., Bedmar, Eulogio J., Mesa, Socorro, and Delgado Igeño, María Jesús

Published
2022

15. Nuevas proteínas implicadas en la producción de NO y N2O por la simbiosis Rhizobium etli-judía

Author

Ministerio de Ciencia e Innovación (España), European Commission, Junta de Andalucía, Hidalgo-García, Alba, Torres Porras, María Jesús, Tortosa Muñoz, Germán, Pacheco, Pedro J., Gates, A., Bedmar, Eulogio J., Girard, Lourdes, Delgado Igeño, María Jesús, Ministerio de Ciencia e Innovación (España), European Commission, Junta de Andalucía, Hidalgo-García, Alba, Torres Porras, María Jesús, Tortosa Muñoz, Germán, Pacheco, Pedro J., Gates, A., Bedmar, Eulogio J., Girard, Lourdes, and Delgado Igeño, María Jesús

Published
2022

16. Efecto del cobre (Cu) en la expresión de genes y proteínas funcionales asociados a la desnitrificación en Bradyrhizobium diazoefficiens

Author

Ministerio de Ciencia e Innovación (España), European Commission, Junta de Andalucía, Pacheco, Pedro J., Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Bedmar, Eulogio J., Mesa, Socorro, Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, Ministerio de Ciencia e Innovación (España), European Commission, Junta de Andalucía, Pacheco, Pedro J., Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Bedmar, Eulogio J., Mesa, Socorro, Tortosa Muñoz, Germán, and Delgado Igeño, María Jesús

Published
2022

17. Análisis transcriptómico de la respuesta de Bradyrhizobium diazoefficiens a la limitación de cobre en condiciones desnitrificantes

Author

Ministerio de Economía y Competitividad (España), European Commission, Junta de Andalucía, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Pacheco, Pedro J., Juárez, R.A., Bedmar, Eulogio J., Mesa, Socorro, Delgado Igeño, María Jesús, Ministerio de Economía y Competitividad (España), European Commission, Junta de Andalucía, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Pacheco, Pedro J., Juárez, R.A., Bedmar, Eulogio J., Mesa, Socorro, and Delgado Igeño, María Jesús

Published
2022

18. Effect of copper on expression of functional genes and proteins associated with Bradyrhizobium diazoefficiens denitrification

Author

CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI), Pacheco, Pedro J., Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Bedmar, Eulogio J., Mesa, Socorro, Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI), Pacheco, Pedro J., Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Bedmar, Eulogio J., Mesa, Socorro, Tortosa Muñoz, Germán, and Delgado Igeño, María Jesús

Abstract

Nitrous oxide (NO) is a powerful greenhouse gas that contributes to climate change. Denitrification is one of the largest sources of NO in soils. The soybean endosymbiont Bradyrhizobium diazoefficiens is a model for rhizobial denitrification studies since, in addition to fixing N, it has the ability to grow anaerobically under free-living conditions by reducing nitrate from the medium through the complete denitrification pathway. This bacterium contains a periplasmic nitrate reductase (Nap), a copper (Cu)-containing nitrite reductase (NirK), a c-type nitric oxide reductase (cNor), and a Cu-dependent nitrous oxide reductase (Nos) encoded by the napEDABC, nirK, norCBQD and nosRZDFYLX genes, respectively. In this work, an integrated study of the role of Cu in B. diazoefficiens denitrification has been performed. A notable reduction in nirK, nor, and nos gene expression observed under Cu limitation was correlated with a significant decrease in NirK, NorC and NosZ protein levels and activities. Meanwhile, nap expression was not affected by Cu, but a remarkable depletion in Nap activity was found, presumably due to an inhibitory effect of nitrite accumulated under Cu-limiting conditions. Interestingly, a post-transcriptional regulation by increasing Nap and NirK activities, as well as NorC and NosZ protein levels, was observed in response to high Cu. Our results demonstrate, for the first time, the role of Cu in transcriptional and post-transcriptional control of B. diazoefficiens denitrification. Thus, this study will contribute by proposing useful strategies for reducing NO emissions from agricultural soils.

Published
2022

19. Ensifer meliloti denitrification is involved in infection effectiveness and N2O emissions from alfalfa root nodules.

Author

Pacheco, Pedro J., Bedmar, Eulogio J., Mesa, Socorro, Tortosa, Germán, and Delgado, María J.

Subjects
*ROOT-tubercles, *NITRITE reductase, *NITRATE reductase, *DENITRIFICATION, *ALFALFA, *AGRICULTURAL climatology
Abstract

Purpose: Alfalfa is one of the most valuable forage crops in temperate climate zones. Ensifer meliloti, the endosymbiont of alfalfa, contains all the denitrification genes but the capacity of alfalfa root nodules to produce nitrous oxide (N2O) is not known. In this work, N2O emissions as well as the influence of bacteroidal denitrification on nodulation competitiveness and N2O release from alfalfa nodules has been investigated. Methods: Medicago sativa cv. Victoria plants were inoculated with E. meliloti 1021, a periplasmic nitrate reductase (Nap) defective mutant, a Nap overexpressing strain and a nitrous oxide reductase defective mutant. Plants were grown in the presence of different nitrate and copper treatments and subjected to flooding during one week before harvesting. N2O production by the nodules was analysed by using gas chromatography. Methyl viologen-dependent nitrate reductase (MV+-NR), nitrite reductase (MV+-NIR) and nitrous oxide reductase (N2OR) enzymatic activities were measured in isolated bacteroids. Results: Alfalfa root nodules produce N2O in response to nitrate and flooding. Overexpression of Nap improved nodulation competitiveness and induced N2O emissions from nodules. Copper is required for an effective symbiosis as well as triggered a reduction of N2O production due to the induction of the N2OR and a reduction of NIR activities in the bacteroids. Conclusion: Alfalfa root nodules emit N2O. Nap is involved in nodulation competitiveness and in N2O emissions by the nodules. Bacteroidal N2OR and NIR activities are modulated by Cu and may be considered as effective targets for the mitigation strategies of N2O emissions derived from alfalfa crops. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

20. Nuevas proteínas implicadas en la producción de NO y N2O por la simbiosis Rhizobium etli-judía

Author

Hidalgo-García, Alba, Torres Porras, María Jesús, Tortosa, Germán, Pacheco, Pedro J., Gates, A., Bedmar, Eulogio J., Girard, Lourdes, Delgado Igeño, María Jesús, Ministerio de Ciencia e Innovación (España), European Commission, and Junta de Andalucía

Abstract

Este trabajo ha sido financiado por el proyecto AGL2017-85676-R, del MCIN/AEI/10.13039/501100011033 y “ERDF A way of making Europe” y el Proyecto P18-RT-1401 de la Junta de Andalucia.

Published
2022

21. Efecto del cobre (Cu) en la expresión de genes y proteínas funcionales asociados a la desnitrificación en Bradyrhizobium diazoefficiens

Author

Pacheco, Pedro J., Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Bedmar, Eulogio J., Mesa, Socorro, Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, Ministerio de Ciencia e Innovación (España), European Commission, and Junta de Andalucía

Abstract

Resumen de la comunicación oral presentada en: XV Reunión Nacional del Metabolismo del Nitrógeno. Córdoba, 2-4 febrero (2022), Este trabajo ha sido financiado por los proyectos AGL2017-85676-R, AGL2015- 63651-P y PID2020-114330GB-100 del MCIN/AEI/ 10.13039/501100011033 y “ERDF A way of making Europe”, y el Proyecto P18-RT-1401 de la Junta de Andalucía. P.J.P es beneficiario de un contrato FPU del Ministerio de Universidades(MIU).

Published
2022

23. Copper involvement in Bradyrhizobium diazoefficiens denitrification

Author

Pacheco, Pedro J., Cabrera, Juan J., Mesa, Socorro, Bedmar, Eulogio J., Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), and Junta de Andalucía

Abstract

Resumen del poster presentado en: The 14th European Nitrogen Fixation Conference. Aarhus University, Dinamarca del 22 septiembre al 22 Octubre (2021), This work was funded by ERDF co-financed grants AGL2O1 7-85676-R and AGL2OI 5-6365 1 -P from Ministerio de Ciencia e Innovación and P18-RT-1401 from Junta de Andalucía. PJP is supported by a FPU fellowship from MCIU (formerly MECD).

Published
2021

24. Identification of new Rhizobium etli enzymes involved in nitric oxide (NO) and nitrous oxide (N2O) emission by common bean nodules

Author

Hidalgo-García, Alba, Torres, M.J., Tortosa Muñoz, Germán, Pacheco, Pedro J., Bedmar, Eulogio J., Girard, L., Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), and Junta de Andalucía

Abstract

Resumen de la comunicación oral presentada en: 8th Plant Nitric Oxide International Meeting. Celebrado en Hungria Szeged. 7-9 Julio 2021, This work was supported by Fondo Europeo de Desarrollo Regional (FEDER)-co-financed grant AGL2017-85676-R from the Ministerio de Ciencia e Innovación (Spain). Grant P18-RT-1401 from the Junta de Andalucía is also acknowledged.

Published
2021

25. Identification of new Rhizobium etli enzymes involved in nitric oxide (NO) and nitrous oxide (N2O) emission by common bean nodules.

Author

European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Hidalgo-García, Alba, Torres, M.J., Tortosa Muñoz, Germán, Pacheco, Pedro J., Bedmar, Eulogio J., Girard, Lourdes, Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Hidalgo-García, Alba, Torres, M.J., Tortosa Muñoz, Germán, Pacheco, Pedro J., Bedmar, Eulogio J., Girard, Lourdes, and Delgado Igeño, María Jesús

Published
2021

26. Copper involvement in Bradyrhizobium diazoefficiens denitrification

Author

European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Pacheco, Pedro J., Cabrera, Juan J., Mesa, Socorro, Bedmar, Eulogio J., Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, European Commission, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Pacheco, Pedro J., Cabrera, Juan J., Mesa, Socorro, Bedmar, Eulogio J., Tortosa Muñoz, Germán, and Delgado Igeño, María Jesús

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results