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3. The relevance of fungi in astrobiology research – Astromycology

Author

Simões, MF, primary, Cortesão, M, additional, Azua-Bustos, A, additional, Bai, F-Y, additional, Canini, F, additional, Casadevall, A, additional, Cassaro, A, additional, Cordero, RJB, additional, Fairén, AG, additional, González-Silva, C, additional, Gunde-Cimerman, N, additional, Koch, S, additional, Liu, X-Z, additional, Onofri, S, additional, Pacelli, C, additional, Selbmann, L, additional, Tesei, D, additional, Waghmode, A, additional, Wang, T, additional, Zucconi, L, additional, and Antunes, A, additional

Published
2023
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8. Erratum: The Italian national project of astrobiology-life in space-origin, presence, persistence of life in space, from molecules to extremophiles (Astrobiology (2020) 20:5 (580-582) DOI: 10.1089/ast.2020.2247)

Author

Onofri S., Balucani N., Barone V., Benedetti P., Billi D., Balbi A., Brucato J. R., Cobucci-Ponzano B., Costanzo G., Rocca N. L., Moracci M., Saladino R., Vladilo G., Albertini N., Battistuzzi M., Bloino J., Botta L., Casavecchia P., Cassaro A., Claudi R., Cocola L., Coduti A., Di Donato P., Di Mauro E., Dore L., Falcinelli S., Fulle M., Ivanovski S., Lombardi A., Mancini G., Maris M., Maurelli L., Murante G., Negri R., Pacelli C., Pagano I., Piccinino D., Poletto L., Prantera G., Puzzarini C., Rampino S., Ripa C., Rosi M., Sanna M., Selbmann L., Silva L., Skouteris D., Strazzulli A., Tasinato N., Timperio A. M., Tozzi A., Tozzi G. P., Trainotti L., Ugliengo P., Vaccaro L., Zucconi L., Onofri S., Balucani N., Barone V., Benedetti P., Billi D., Balbi A., Brucato J.R., Cobucci-Ponzano B., Costanzo G., Rocca N.L., Moracci M., Saladino R., Vladilo G., Albertini N., Battistuzzi M., Bloino J., Botta L., Casavecchia P., Cassaro A., Claudi R., Cocola L., Coduti A., Di Donato P., Di Mauro E., Dore L., Falcinelli S., Fulle M., Ivanovski S., Lombardi A., Mancini G., Maris M., Maurelli L., Murante G., Negri R., Pacelli C., Pagano I., Piccinino D., Poletto L., Prantera G., Puzzarini C., Rampino S., Ripa C., Rosi M., Sanna M., Selbmann L., Silva L., Skouteris D., Strazzulli A., Tasinato N., Timperio A.M., Tozzi A., Tozzi G.P., Trainotti L., Ugliengo P., Vaccaro L., Zucconi L., Onofri, S., Balucani, N., Barone, V., Benedetti, P., Billi, D., Balbi, A., Brucato, J. R., Cobucci-Ponzano, B., Costanzo, G., Rocca, N. L., Moracci, M., Saladino, R., Vladilo, G., Albertini, N., Battistuzzi, M., Bloino, J., Botta, L., Casavecchia, P., Cassaro, A., Claudi, R., Cocola, L., Coduti, A., Di Donato, P., Di Mauro, E., Dore, L., Falcinelli, S., Fulle, M., Ivanovski, S., Lombardi, A., Mancini, G., Maris, M., Maurelli, L., Murante, G., Negri, R., Pacelli, C., Pagano, I., Piccinino, D., Poletto, L., Prantera, G., Puzzarini, C., Rampino, S., Ripa, C., Rosi, M., Sanna, M., Selbmann, L., Silva, L., Skouteris, D., Strazzulli, A., Tasinato, N., Timperio, A. M., Tozzi, A., Tozzi, G. P., Trainotti, L., Ugliengo, P., Vaccaro, L., and Zucconi, L.

Subjects
life in space-origin, astrobiology, persistence of life in space, from molecules to extremophiles in space, Settore CHIM/02 - Chimica Fisica
Abstract

The Italian National Project of Astrobiology―Life in Space―Origin, Presence, Persistence of Life in Space, from Molecules to Extremophiles A member’s name should be added to the OPPS Project Team: Stavro Ivanovski on page 580 footnotes. We have made this correction on the title page of the article and the eTOC.

Published
2020

9. 'How Would You Communicate With a Robot?': People with Neourodevelopmental Disorder's Perspective

Author

Pacelli, C, Geeganag, T, Spitalepolitecnico, M, Beccaluva, E, Garzotto, F, Geeganag, TKDPH, SpitalePolitecnico, M, Pacelli, C, Geeganag, T, Spitalepolitecnico, M, Beccaluva, E, Garzotto, F, Geeganag, TKDPH, and SpitalePolitecnico, M

Abstract

Neurodevelopmental disorders (NDDs) are characterised by impairments in communication. Socially assistive robots have been identified as a promising avenue to alleviate their burden. Since NDDs have different needs, their way of communicating with a robot (e.g., speech-based) could differ among individuals. This paper aims to investigate the most suitable modality to communicate with a robot for NDDs among voice, cards, and buttons - and explore their opinion on this matter. We ran an exploratory study involving 29 NDDs participants: 13 of them could freely communicate with an autonomous QT robot, 9 took part in a group discussion, and 7 first interacted individually with the robot, and then they participated in a group discussion. Our results showed that i) the cards were the most used communication modality, ii) voice can be used for counting games, buttons for multiple-choice games, and cards for memory-like games, iii) opinions did not differ much among groups.*

Published
2022

14. Sensory Properties and Consumer Liking of Buffalo Stracchino Cheese

Author

Di Cairano, M., Pacelli, C., Bragaglio, A., Napolitano, F., Braghieri, A., Di Cairano, M., Pacelli, C., Bragaglio, A., Napolitano, F., and Braghieri, A.

Abstract

The present study aims to characterize buffalo Stracchino cheese (BS) from a sensory point of view and verify how much consumers like it compared with the standard Stracchino cheese obtained from cow milk (CS). Nine panelists specifically trained to evaluate Stracchino cheese were used to conduct a quantitative descriptive sensory analysis, whereas 80 untrained consumers balanced for gender participated in the hedonic consumer test. Stracchino appearance was affected by milk type with higher intensities perceived for BS in terms of whiteness (P<0.0001) and shininess (P<0.001). As to taste and texture, BS showed higher sourness and oiliness intensities than CS, respectively (P<0.0001). Milk type did not affect the overall liking or the liking in terms of taste/flavor, texture, and appearance, but consumers rated both products at scores well above the neutral point. In addition, the liking expressed in blind conditions (i.e., without information on the milk type) was significantly lower as compared with the liking elicited by the expectations (i.e., based only on the information on the milk type) (P<0.05 and P<0.10, for CS and BS, respectively). We conclude that the good eating quality of buffalo Stracchino cheese as assessed by the consumer panel and the lack of differences between CS and BS in terms of a consumer may anticipate a possible good positioning of this novel product in the market of fresh cheese.

Published
2021

15. The Nutriss Study: A New Approach to Calibrate Diet and Exercise in Long-Term Space Missions to Maintain Body Fat, Muscle and Fluid Homeostasis

Author

Di Girolamo, F. G., primary, Biolo, G., additional, Fiotti, N., additional, Situlin, R., additional, Piacenza, C., additional, Lepore, P., additional, Fortezza, R., additional, Carrubba, E., additional, Pacelli, C., additional, Valentini, G., additional, Mascetti, G., additional, and Piccirillo, S., additional

Published
2020
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18. Parkin mutation affects clock gene-dependent energy metabolism

Author

Pacelli, C, Rotundo, G, Lecce, L, Menga, M, Bidollari, E, Scrima, R, Cela, O, Piccoli, C, Cocco, T, Vescovi, A, Mazzoccoli, G, Rosati, J, Capitanio, N, Vescovi, AL, Pacelli, C, Rotundo, G, Lecce, L, Menga, M, Bidollari, E, Scrima, R, Cela, O, Piccoli, C, Cocco, T, Vescovi, A, Mazzoccoli, G, Rosati, J, Capitanio, N, and Vescovi, AL

Abstract

Growing evidence highlights a tight connection between circadian rhythms, molecular clockworks, and mitochondrial function. In particular, mitochondrial quality control and bioenergetics have been proven to undergo circadian oscillations driven by core clock genes. Parkinson's disease (PD) is a chronic neurodegenerative disease characterized by a selective loss of dopaminergic neurons. Almost half of the autosomal recessive forms of juvenile parkinsonism have been associated with mutations in the PARK2 gene coding for parkin, shown to be involved in mitophagy-mediated mitochondrial quality control. The aim of this study was to investigate, in fibroblasts from genetic PD patients carrying parkin mutations, the interplay between mitochondrial bioenergetics and the cell autonomous circadian clock. Using two different in vitro synchronization protocols, we demonstrated that normal fibroblasts displayed rhythmic oscillations of both mitochondrial respiration and glycolytic activity. Conversely, in fibroblasts obtained from PD patients, a severe damping of the bioenergetic oscillatory patterns was observed. Analysis of the core clock genes showed deregulation of their expression patterns in PD fibroblasts, which was confirmed in induced pluripotent stem cells (iPSCs) and induced neural stem cells (iNSCs) derived thereof. The results from this study support a reciprocal interplay between the clockwork machinery and mitochondrial energy metabolism, point to a parkin-dependent mechanism of regulation, and unveil a hitherto unappreciated level of complexity in the pathophysiology of PD and eventually other neurodegenerative diseases.

Published
2019

20. Test-retest reliability of buffalo response to humans

Author

DE ROSA, GIUSEPPE, GRASSO, FERNANDO, BRAGHIERI A., BRAGAGLIO A., PACELLI C., NAPOLITANO F., Cathy Dwyer, Marie Haskell, Victoria Sandilands, DE ROSA, Giuseppe, Grasso, Fernando, Braghieri, A., Bragaglio, A., Pacelli, C., and Napolitano, F.

Subjects
dairy buffalo, human-animal relationship, behaviour, test-retest reliability
Abstract

This study aims to assess the test-retest reliability of buffalo response to humans and the relationship between the behaviour of stockpeople and buffalo. The research was carried out in 27 buffalo farms located in southern Italy. Milk production was recorded only in the 14 farms enrolled in the milk recording scheme. Before assessment informed consent was obtained by stockpeople. Human–animal relationship was assessed through two different tests: behavioural observations of stockperson and animals during milking and avoidance distance at the manger. These tests were repeated within one month to assess test–retest reliability. Stockpeople attitude was evaluated using a questionnaire divided into four sections (general beliefs about buffaloes, general beliefs about working with buffaloes, behavioural intentions with respect to interacting with buffaloes and job satisfaction) including 21 statements. A high degree of test–retest reliability was observed for all the variables concerning the behaviour of stockpeople and animals. The values of Spearman rank correlation coefficient (rs) ranged from 0.578 (P

Published
2016

21. Draft Genome Sequences of the Antarctic Endolithic Fungi Rachicladosporium antarcticum CCFEE 5527 and Rachicladosporium sp. CCFEE 5018

Author

Coleine, C, Masonjones, S, Selbmann, L, Zucconi, L, Onofri, S, Pacelli, C, and Stajich, JE

Abstract

The draft genome sequences of Rachicladosporium antarcticum CCFEE 5527 and Rachicladosporium sp. CCFEE 5018 are the first sequenced genomes from this genus, which comprises rock-inhabiting fungi. These endolithic strains were isolated from inside rocks collected from the Antarctic Peninsula and Battleship Promontory (McMurdo Dry Valleys), Antarctica, respectively.

Published
2017

22. The BIOMEX space experiment:, biosignatures detected after ground-based Science Verification Tests (SVT) in space and simulated Mars conditions

Author

Onofri, S., Pacelli, C., Selbmann, L., Zucconi, L., Saladino, R., Botta, L., Rabbow, Elke, Boettger, U., and de Vera, J.-P.

Subjects
Strahlenbiologie, Leitungsbereich PF, Mars Experiment (BIOMEX), Institut für Optische Sensorsysteme, space mission EXPOSE-R2
Abstract

The BIOlogy and Mars Experiment (BIOMEX) is part of the European Space Agency (ESA) space mission EXPOSE-R2 in Low Earth Orbit (LEO), aiming to expose microorganisms for 1.5 years to space and simulated Mars-like conditions on the International Space Station (ISS). In preparation of this mission, dried colonies of the Antarctic cryptoendolithic black fungus Cryomyces antarcticus CCFEE 515, grown on Martian and Lunar analogue regolith pellets, were subjected to several ground-based preflight tests, Experiment Verification Tests (EVTs) and Science Verification Tests (SVTs). These tests aimed to verify i) the resistance of our model organism to space stressors when grown on extraterrestrial rock analogues and ii) the possibility to detect biomolecules as potential biosignatures in reference to support the Exomars 2020 mission. Here some results are reported showing the outcome of the SVTs, the last set of experiments, where the effect of UV radiation was analyzed if combined with simulated space vacuum or simulated Mars-like conditions. The analyses performed by Gas Chromatography-Mass Spectrometry showed the presence of fungal metabolites, as azelaic acid, that remain unaltered after the different expositions and treatments. In addition first results of Raman Spectroscopy analysis on melanin will be presented. Further investigation is necessary to derive the appropriate parameter set for Raman spectroscopy of melanin. Transmission Electron Microscopy (TEM) observations showed different results in preservation of cell’s ultrastructure.

Published
2017

31. Studio delle relazioni uomo-animale nella specie caprina

Author

BRAGAGLIO A., SERRAPICA M., NAPOLITANO F., GIROLAMI A., PACELLI C., BRAGHIERI A., DE ROSA, GIUSEPPE, S.I.P.A.O.C., Bragaglio, A., Serrapica, M., Napolitano, F., Girolami, A., Pacelli, C., DE ROSA, Giuseppe, and Braghieri, A.

Subjects
sheep, Animal welfare, avoidance distance, behaviour
Abstract

La presente indagine è finalizzata alla messa a punto di metodi di valutazione del rapporto uomo – animale da utilizzare per il monitoraggio del benessere delle capre in lattazione. Pertanto, sono state valutate le relazioni esistenti fra il comportamento degli operatori nel corso della mungitura e le reazioni degli animali all’uomo. La prova ha riguardato 14 aziende caprine, ubicate nella provincia di Potenza. In ogni azienda è stato condotto un test di approccio, che ha permesso di valutare il numero di animali che si lasciano toccare in un periodo di 2 minuti (Touch2min) camminando lentamente (circa 1 m/sec), e due test di avvicinamento (distanza di fuga alla mangiatoia e nel box di allevamento). Questi ultimi consistono nell’avvicinarsi lentamente (circa 1 m/sec) a un animale (rispettivamente alla mangiatoia o nel box di allevamento) con il braccio leggermente sporgente in avanti, fino a quando il soggetto si ritrae. Nel corso di questi test sono state rilevate le seguenti variabili: distanza di fuga alla mangiatoia (ADM), distanza di fuga nel box (ADP), numero di soggetti che si lasciano toccare alla mangiatoia (DF0M) e numero di soggetti che si lasciano toccare nel box (DF0P). In sala di mungitura sono stati valutati il comportamento del mungitore, in termini di interazioni positive (POS INT), neutre (NEUT INT) e negative (NEG INT), e quello degli animali durante la mungitura come numero di scalci (Kicks). Sono state considerate come interazioni positive il parlare a bassa voce, l’accarezzare e il toccare gentilmente l’animale; sono state classificate come interazioni neutre fischiare, battere le mani, colpire le partizioni con bastoni o oggetti simili, parlare in modo determinato e deciso, spingere con la mano e toccare con bastoni o oggetti simili; mentre sono state considerate negative le seguenti interazioni: gridare, parlare manifestando impazienza, le spinte e i colpi con le mani o con i bastoni. Sono state evidenziate delle correlazioni positive fra le interazioni neutre, che in ogni caso non comprendono atteggiamenti favorevoli nei confronti degli animali, e il numero di scalci durante la mungitura (rs=0,477, P>0,05) e fra le interazioni negative e la distanza di fuga nel box (rs=0,537, P

Published
2014

32. Assessment of human-animal relationship in dairy buffaloes

Author

DE ROSA, GIUSEPPE, GRASSO, FERNANDO, MASUCCI, FELICIA, BRAGAGLIO A., PACELLI C., NAPOLITANO F., Inma Estevez, DE ROSA, Giuseppe, Grasso, Fernando, Masucci, Felicia, Bragaglio, A., Pacelli, C., and Napolitano, F.

Subjects
dairy buffalo, human-animal relationship, behaviour
Abstract

The present study was aimed to assess the relationship among stockperson behaviour and buffalo behaviour. The research was carried out in 17 buffalo farms located in southern Italy. Observations were conducted by two trained assessors. Human–animal relationship was assessed performing two different tests: behavioural observations of stockperson and animals during milking and avoidance distance at the manger. These tests were repeated within one month to assess test–retest reliability. Stockpeople attitude was also evaluated using a questionnaire divided into four sections (general beliefs about buffaloes, general beliefs about working with buffaloes, behavioural intentions with respect to interacting with buffaloes and job satisfaction) including 21 statements. A high degree of test–retest reliability was observed for all the variables concerning the behaviour of stockperson and animals. The values of coefficients (Spearman rank correlation coefficient: rs)) ranged from 0.587 (P

Published
2014

33. Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

Author

Klionsky, D.J. Abdelmohsen, K. Abe, A. Abedin, M.J. Abeliovich, H. Arozena, A.A. Adachi, H. Adams, C.M. Adams, P.D. Adeli, K. Adhihetty, P.J. Adler, S.G. Agam, G. Agarwal, R. Aghi, M.K. Agnello, M. Agostinis, P. Aguilar, P.V. Aguirre-Ghiso, J. Airoldi, E.M. Ait-Si-Ali, S. Akematsu, T. Akporiaye, E.T. Al-Rubeai, M. Albaiceta, G.M. Albanese, C. Albani, D. Albert, M.L. Aldudo, J. Algül, H. Alirezaei, M. Alloza, I. Almasan, A. Almonte-Beceril, M. Alnemri, E.S. Alonso, C. Altan-Bonnet, N. Altieri, D.C. Alvarez, S. Alvarez-Erviti, L. Alves, S. Amadoro, G. Amano, A. Amantini, C. Ambrosio, S. Amelio, I. Amer, A.O. Amessou, M. Amon, A. An, Z. Anania, F.A. Andersen, S.U. Andley, U.P. Andreadi, C.K. Andrieu-Abadie, N. Anel, A. Ann, D.K. Anoopkumar-Dukie, S. Antonioli, M. Aoki, H. Apostolova, N. Aquila, S. Aquilano, K. Araki, K. Arama, E. Aranda, A. Araya, J. Arcaro, A. Arias, E. Arimoto, H. Ariosa, A.R. Armstrong, J.L. Arnould, T. Arsov, I. Asanuma, K. Askanas, V. Asselin, E. Atarashi, R. Atherton, S.S. Atkin, J.D. Attardi, L.D. Auberger, P. Auburger, G. Aurelian, L. Autelli, R. Avagliano, L. Avantaggiati, M.L. Avrahami, L. Azad, N. Awale, S. Bachetti, T. Backer, J.M. Bae, D.-H. Bae, J.-S. Bae, O.-N. Bae, S.H. Baehrecke, E.H. Baek, S.-H. Baghdiguian, S. Bagniewska-Zadworna, A. Bai, H. Bai, J. Bai, X.-Y. Bailly, Y. Balaji, K.N. Balduini, W. Ballabio, A. Balzan, R. Banerjee, R. Bánhegyi, G. Bao, H. Barbeau, B. Barrachina, M.D. Barreiro, E. Bartel, B. Bartolomé, A. Bassham, D.C. Bassi, M.T. Bast, R.C., Jr. Basu, A. Batista, M.T. Batoko, H. Battino, M. Bauckman, K. Baumgarner, B.L. Bayer, K.U. Beale, R. Beaulieu, J.-F. Beck, G.R., Jr. Becker, C. Beckham, J.D. Bédard, P.-A. Bednarski, P.J. Begley, T.J. Behl, C. Behrends, C. Behrens, G.M.N. Behrns, K.E. Bejarano, E. Belaid, A. Belleudi, F. Bénard, G. Berchem, G. Bergamaschi, D. Bergami, M. Berkhout, B. Berliocchi, L. Bernard, A. Bernard, M. Bernassola, F. Bertolotti, A. Bess, A.S. Besteiro, S. Bettuzzi, S. Bhalla, S. Bhattacharyya, S. Bhutia, S.K. Biagosch, C. Bianchi, M.W. Biard-Piechaczyk, M. Billes, V. Bincoletto, C. Bingol, B. Bird, S.W. Bitoun, M. Bjedov, I. Blackstone, C. Blanc, L. Blanco, G.A. Blomhoff, H.K. Boada-Romero, E. Böckler, S. Boes, M. Boesze-Battaglia, K. Boise, L.H. Bolino, A. Boman, A. Bonaldo, P. Bordi, M. Bosch, J. Botana, L.M. Botti, J. Bou, G. Bouché, M. Bouchecareilh, M. Boucher, M.-J. Boulton, M.E. Bouret, S.G. Boya, P. Boyer-Guittaut, M. Bozhkov, P.V. Brady, N. Braga, V.M.M. Brancolini, C. Braus, G.H. Bravo-San-Pedro, J.M. Brennan, L.A. Bresnick, E.H. Brest, P. Bridges, D. Bringer, M.-A. Brini, M. Brito, G.C. Brodin, B. Brookes, P.S. Brown, E.J. Brown, K. Broxmeyer, H.E. Bruhat, A. Brum, P.C. Brumell, J.H. Brunetti-Pierri, N. Bryson-Richardson, R.J. Buch, S. Buchan, A.M. Budak, H. Bulavin, D.V. Bultman, S.J. Bultynck, G. Bumbasirevic, V. Burelle, Y. Burke, R.E. Burmeister, M. Bütikofer, P. Caberlotto, L. Cadwell, K. Cahova, M. Cai, D. Cai, J. Cai, Q. Calatayud, S. Camougrand, N. Campanella, M. Campbell, G.R. Campbell, M. Campello, S. Candau, R. Caniggia, I. Cantoni, L. Cao, L. Caplan, A.B. Caraglia, M. Cardinali, C. Cardoso, S.M. Carew, J.S. Carleton, L.A. Carlin, C.R. Carloni, S. Carlsson, S.R. Carmona-Gutierrez, D. Carneiro, L.A.M. Carnevali, O. Carra, S. Carrier, A. Carroll, B. Casas, C. Casas, J. Cassinelli, G. Castets, P. Castro-Obregon, S. Cavallini, G. Ceccherini, I. Cecconi, F. Cederbaum, A.I. Ceña, V. Cenci, S. Cerella, C. Cervia, D. Cetrullo, S. Chaachouay, H. Chae, H.-J. Chagin, A.S. Chai, C.-Y. Chakrabarti, G. Chamilos, G. Chan, E.Y.W. Chan, M.T.V. Chandra, D. Chandra, P. Chang, C.-P. Chang, R.C.-C. Chang, T.Y. Chatham, J.C. Chatterjee, S. Chauhan, S. Che, Y. Cheetham, M.E. Cheluvappa, R. Chen, C.-J. Chen, G. Chen, G.-C. Chen, G. Chen, H. Chen, J.W. Chen, J.-K. Chen, M. Chen, M. Chen, P. Chen, Q. Chen, Q. Chen, S.-D. Chen, S. Chen, S.S.-L. Chen, W. Chen, W.-J. Chen, W.Q. Chen, W. Chen, X. Chen, Y.-H. Chen, Y.-G. Chen, Y. Chen, Y. Chen, Y. Chen, Y.-J. Chen, Y.-Q. Chen, Y. Chen, Z. Chen, Z. Cheng, A. Cheng, C.H.K. Cheng, H. Cheong, H. Cherry, S. Chesney, J. Cheung, C.H.A. Chevet, E. Chi, H.C. Chi, S.-G. Chiacchiera, F. Chiang, H.-L. Chiarelli, R. Chiariello, M. Chieppa, M. Chin, L.-S. Chiong, M. Chiu, G.N.C. Cho, D.-H. Cho, S.-G. Cho, W.C. Cho, Y.-Y. Cho, Y.-S. Choi, A.M.K. Choi, E.-J. Choi, E.-K. Choi, J. Choi, M.E. Choi, S.-I. Chou, T.-F. Chouaib, S. Choubey, D. Choubey, V. Chow, K.-C. Chowdhury, K. Chu, C.T. Chuang, T.-H. Chun, T. Chung, H. Chung, T. Chung, Y.-L. Chwae, Y.-J. Cianfanelli, V. Ciarcia, R. Ciechomska, I.A. Ciriolo, M.R. Cirone, M. Claerhout, S. Clague, M.J. Cl� ria, J. Clarke, P.G.H. Clarke, R. Clementi, E. Cleyrat, C. Cnop, M. Coccia, E.M. Cocco, T. Codogno, P. Coers, J. Cohen, E.E.W. Colecchia, D. Coletto, L. Coll, N.S. Colucci-Guyon, E. Comincini, S. Condello, M. Cook, K.L. Coombs, G.H. Cooper, C.D. Cooper, J.M. Coppens, I. Corasaniti, M.T. Corazzari, M. Corbalan, R. Corcelle-Termeau, E. Cordero, M.D. Corral-Ramos, C. Corti, O. Cossarizza, A. Costelli, P. Costes, S. Cotman, S.L. Coto-Montes, A. Cottet, S. Couve, E. Covey, L.R. Cowart, L.A. Cox, J.S. Coxon, F.P. Coyne, C.B. Cragg, M.S. Craven, R.J. Crepaldi, T. Crespo, J.L. Criollo, A. Crippa, V. Cruz, M.T. Cuervo, A.M. Cuezva, J.M. Cui, T. Cutillas, P.R. Czaja, M.J. Czyzyk-Krzeska, M.F. Dagda, R.K. Dahmen, U. Dai, C. Dai, W. Dai, Y. Dalby, K.N. Valle, L.D. Dalmasso, G. D'amelio, M. Damme, M. Darfeuille-Michaud, A. Dargemont, C. Darley-Usmar, V.M. Dasarathy, S. Dasgupta, B. Dash, S. Dass, C.R. Davey, H.M. Davids, L.M. Dávila, D. Davis, R.J. Dawson, T.M. Dawson, V.L. Daza, P. de Belleroche, J. de Figueiredo, P. de Figueiredo, R.C.B.Q. de la Fuente, J. De Martino, L. De Matteis, A. De Meyer, G.R.Y. De Milito, A. De Santi, M. de Souza, W. De Tata, V. De Zio, D. Debnath, J. Dechant, R. Decuypere, J.-P. Deegan, S. Dehay, B. Del Bello, B. Del Re, D.P. Delage-Mourroux, R. Delbridge, L.M.D. Deldicque, L. Delorme-Axford, E. 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Published
2016

34. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy

Author

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Published
2016

35. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Author

Klionsky, D.J. Abdelmohsen, K. Abe, A. Abedin, M.J. Abeliovich, H. Arozena, A.A. Adachi, H. Adams, C.M. Adams, P.D. Adeli, K. Adhihetty, P.J. Adler, S.G. Agam, G. Agarwal, R. Aghi, M.K. Agnello, M. Agostinis, P. Aguilar, P.V. Aguirre-Ghiso, J. Airoldi, E.M. Ait-Si-Ali, S. Akematsu, T. Akporiaye, E.T. Al-Rubeai, M. Albaiceta, G.M. Albanese, C. Albani, D. Albert, M.L. Aldudo, J. Algül, H. Alirezaei, M. Alloza, I. Almasan, A. Almonte-Beceril, M. Alnemri, E.S. Alonso, C. Altan-Bonnet, N. Altieri, D.C. Alvarez, S. Alvarez-Erviti, L. Alves, S. Amadoro, G. Amano, A. Amantini, C. Ambrosio, S. Amelio, I. Amer, A.O. Amessou, M. Amon, A. An, Z. Anania, F.A. Andersen, S.U. Andley, U.P. Andreadi, C.K. Andrieu-Abadie, N. Anel, A. Ann, D.K. Anoopkumar-Dukie, S. Antonioli, M. Aoki, H. Apostolova, N. Aquila, S. Aquilano, K. Araki, K. Arama, E. Aranda, A. Araya, J. Arcaro, A. Arias, E. Arimoto, H. Ariosa, A.R. Armstrong, J.L. Arnould, T. Arsov, I. Asanuma, K. Askanas, V. Asselin, E. Atarashi, R. 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Published
2016

36. Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

Author

Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., Adhihetty, P. J., Adler, S. G., Agam, G., Agarwal, R., Aghi, M. K., Agnello, M., Agostinis, P., Aguilar, P. V., Aguirre-Ghiso, J., Airoldi, E. M., Ait-Si-Ali, S., Akematsu, T., Akporiaye, E. T., Al-Rubeai, M., Albaiceta, G. M., Albanese, C., Albani, D., Albert, M. L., Aldudo, J., Algül, H., Alirezaei, M., Alloza, I., Almasan, A., Almonte-Beceril, M., Alnemri, E. S., Alonso, C., Altan-Bonnet, N., Altieri, D. C., Alvarez, S., Alvarez-Erviti, L., Alves, S., Amadoro, G., Amano, A., Amantini, C., Ambrosio, S., Amelio, I., Amer, A. O., Amessou, M., Amon, A., An, Z., Anania, F. A., Andersen, S. U., Andley, U. P., Andreadi, C. K., Andrieu-Abadie, N., Anel, A., Ann, D. K., Anoopkumar-Dukie, S., Antonioli, M., Aoki, H., Apostolova, N., Aquila, S., Aquilano, K., Araki, K., Arama, E., Aranda, A., Araya, J., Arcaro, A., Arias, E., Arimoto, H., Ariosa, A. R., Armstrong, J. L., Arnould, T., Arsov, I., Asanuma, K., Askanas, V., Asselin, E., Atarashi, R., Atherton, S. S., Atkin, J. D., Attardi, L. D., Auberger, P., Auburger, G., Aurelian, L., Autelli, R., Avagliano, L., Avantaggiati, M. L., Avrahami, L., Azad, N., Awale, S., Bachetti, T., Backer, J. M., Bae, D. -H., Bae, J. -S., Bae, O. -N., Bae, S. H., Baehrecke, E. H., Baek, S. -H., Baghdiguian, S., Bagniewska-Zadworna, A., Bai, H., Bai, J., Bai, X. -Y., Bailly, Y., Balaji, K. N., Balduini, W., Ballabio, A., Balzan, R., Banerjee, R., Bánhegyi, G., Bao, H., Barbeau, B., Barrachina, M. D., Barreiro, E., Bartel, B., Bartolomé, A., Bassham, D. C., Bassi, M. T., Bast, R. C., J, R., Basu, A., Batista, M. T., Batoko, H., Battino, M., Bauckman, K., Baumgarner, B. L., Bayer, K. U., Beale, R., Beaulieu, J. -F., Beck, G. R., Becker, C., Beckham, J. D., Bédard, P. -A., Bednarski, P. J., Begley, T. J., Behl, C., Behrends, C., Behrens, G. M. N., Behrns, K. E., Bejarano, E., Belaid, A., Belleudi, F., Bénard, G., Berchem, G., Bergamaschi, D., Bergami, M., Berkhout, B., Berliocchi, L., Bernard, A., Bernard, M., Bernassola, F., Bertolotti, A., Bess, A. S., Besteiro, S., Bettuzzi, S., Bhalla, S., Bhattacharyya, S., Bhutia, S. K., Biagosch, C., Bianchi, M. W., Biard-Piechaczyk, M., Billes, V., Bincoletto, C., Bingol, B., Bird, S. W., Bitoun, M., Bjedov, I., Blackstone, C., Blanc, L., Blanco, G. A., Blomhoff, H. K., Boada-Romero, E., Böckler, S., Boes, M., Boesze-Battaglia, K., Boise, L. H., Bolino, A., Boman, A., Bonaldo, P., Bordi, M., Bosch, J., Botana, L. M., Botti, J., Bou, G., Bouché, M., Bouchecareilh, M., Boucher, M. -J., Boulton, M. E., Bouret, S. G., Boya, P., Boyer-Guittaut, M., Bozhkov, P. V., Brady, N., Braga, V. M. M., Brancolini, C., Braus, G. H., Bravo-San-Pedro, J. M., Brennan, L. A., Bresnick, E. H., Brest, P., Bridges, D., Bringer, M. -A., Brini, M., Brito, G. C., Brodin, B., Brookes, P. S., Brown, E. J., Brown, K., Broxmeyer, H. E., Bruhat, A., Brum, P. C., Brumell, J. H., Brunetti-Pierri, N., Bryson-Richardson, R. J., Buch, S., Buchan, A. M., Budak, H., Bulavin, D. V., Bultman, S. J., Bultynck, G., Bumbasirevic, V., Burelle, Y., Burke, R. E., Burmeister, M., Bütikofer, P., Caberlotto, L., Cadwell, K., Cahova, M., Cai, D., Cai, J., Cai, Q., Calatayud, S., Camougrand, N., Campanella, M., Campbell, G. R., Campbell, M., Campello, S., Candau, R., Caniggia, I., Cantoni, L., Cao, L., Caplan, A. B., Caraglia, M., Cardinali, C., Cardoso, S. M., Carew, J. S., Carleton, L. A., Carlin, C. R., Carloni, S., Carlsson, S. R., Carmona-Gutierrez, D., Carneiro, L. A. M., Carnevali, O., Carra, S., Carrier, A., Carroll, B., Casas, C., Casas, J., Cassinelli, G., Castets, P., Castro-Obregon, S., Cavallini, G., Ceccherini, I., Cecconi, F., Cederbaum, A. I., Ceña, V., Cenci, S., Cerella, C., Cervia, D., Cetrullo, S., Chaachouay, H., Chae, H. -J., Chagin, A. 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J., Di Bartolomeo, S., Di Daniele, N., Di Domenico, F., Di Nardo, A., Di Paola, S., Di Pietro, A., Di Renzo, L., Di Antonio, A., Díaz-Araya, G., Díaz-Laviada, I., Diaz-Meco, M. T., Diaz-Nido, J., Dickey, C. A., Dickson, R. C., Diederich, M., Digard, P., Dikic, I., Dinesh-Kumar, S. P., Ding, C., Ding, W. -X., Ding, Z., Dini, L., Distler, J. H. W., Diwan, A., Djavaheri-Mergny, M., Dmytruk, K., Dobson, R. C. J., Doetsch, V., Dokladny, K., Dokudovskaya, S., Donadelli, M., Dong, X. C., Dong, X., Dong, Z., Donohue, T. M., Donohue-Jr, T. M., Doran, K. S., D'Orazi, G., Dorn, G. W., Dosenko, V., Dridi, S., Drucker, L., Du, J., L. -L., Du, Du, L., du Toit, A., Dua, P., Duan, L., Duann, P., Dubey, V. K., Duchen, M. R., Duchosal, M. A., Duez, H., Dugail, I., Dumit, V. I., Duncan, M. C., Dunlop, E. A., Dunn, W. A., Dupont, N., Dupuis, L., Durán, R. V., Durcan, T. M., Duvezin-Caubet, S., Duvvuri, U., Eapen, V., Ebrahimi-Fakhari, D., Echard, A., Eckhart, L., Edelstein, C. L., Edinger, A. 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H., Finkbeiner, S., Finkel, T., Fiorito, F., Fisher, P. B., Flajolet, M., Flamigni, F., Florey, O., Florio, S., Floto, R. A., Folini, M., Follo, C., Fon, E. A., Fornai, F., Fortunato, F., Fraldi, A., Franco, R., Francois, A., François, A., Frankel, L. B., Fraser, I. D. C., Frey, N., Freyssenet, D. G., Frezza, C., Friedman, S. L., Frigo, D. E., Fu, D., Fuentes, J. M., Fueyo, J., Fujitani, Y., Fujiwara, Y., Fujiya, M., Fukuda, M., Fulda, S., Fusco, C., Gabryel, B., Gaestel, M., Gailly, P., Gajewska, M., Galadari, S., Galili, G., Galindo, I., Galindo, M. F., Galliciotti, G., Galluzzi, L., Galy, V., Gammoh, N., Gandy, S., Ganesan, A. K., Ganesan, S., Ganley, I. G., Gannagé, M., Gao, F. -B., Gao, F., Gao, J. -X., Nannig, L. G., Véscovi, E. G., Garcia-Macía, M., Garcia-Ruiz, C., Garg, A. D., Garg, P. K., Gargini, R., Gassen, N. C., Gatica, D., Gatti, E., Gavard, J., Gavathiotis, E., Ge, L., Ge, P., Ge, S., Gean, P. -W., Gelmetti, V., Genazzani, A. A., Geng, J., Genschik, P., Gerner, L., Gestwicki, J. E., Gewirtz, D. A., Ghavami, S., Ghigo, E., Ghosh, D., Giammarioli, A. M., Giampieri, F., Giampietri, C., Giatromanolaki, A., Gibbings, D. J., Gibellini, L., Gibson, S. B., Ginet, V., Giordano, A., Giorgini, F., Giovannetti, E., Girardin, S. E., Gispert, S., Giuliano, S., Gladson, C. L., Glavic, A., Gleave, M., Godefroy, N., Gogal, R. M., Gokulan, K., Goldman, G. H., Goletti, D., Goligorsky, M. S., Gomes, A. V., Gomes, L. C., Gomez, H., Gomez-Manzano, C., Gómez-Sánchez, R., Gonçalves, D. A. P., Goncu, E., Gong, Q., Gongora, C., Gonzalez, C. B., Gonzalez-Alegre, P., Gonzalez-Cabo, P., González-Polo, R. A., Goping, I. S., Gorbea, C., Gorbunov, N. V., Goring, D. R., Gorman, A. M., Gorski, S. M., Goruppi, S., Goto-Yamada, S., Gotor, C., Gottlieb, R. A., Gozes, I., Gozuacik, D., Graba, Y., Graef, M., Granato, G. E., Grant, G. D., Grant, S., Gravina, G. L., Green, D. R., Greenhough, A., Greenwood, M. T., Grimaldi, B., Gros, F., Grose, C., Groulx, J. -F., Gruber, F., Grumati, P., Grune, T., Guan, J. -L., Guan, K. -L., Guerra, B., Guillen, C., Gulshan, K., Gunst, J., Guo, C., Guo, L., Guo, M., Guo, W., Guo, X. -G., Gust, A. A., Gustafsson, Å. B., Gutierrez, E., Gutierrez, M. G., Gwak, H. -S., Haas, A., Haber, J. E., Hadano, S., Hagedorn, M., Hahn, D. R., Halayko, A. J., Hamacher-Brady, A., Hamada, K., Hamai, A., Hamann, A., Hamasaki, M., Hamer, I., Hamid, Q., Hammond, E. M., Han, F., Han, W., Handa, J. T., Hanover, J. A., Hansen, M., Harada, M., Harhaji-Trajkovic, L., Harper, J. W., Harrath, A. H., Harris, A. L., Harris, J., Hasler, U., Hasselblatt, P., Hasui, K., Hawley, R. G., Hawley, T. S., He, C., C. Y., He, He, F., He, G., R. -R., He, X. -H., He, Y. -W., He, Y. -Y., He, Heath, J. K., Hébert, M. -J., Heinzen, R. A., Helgason, G. V., Hensel, M., Henske, E. P., Her, C., Herman, P. K., Hernández, A., Hernandez, C., Hernández-Tiedra, S., Hetz, C., Hiesinger, P. 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H., Kandouz, M., Kang, C., Kang, R., Kang, T. -C., Kanki, T., Kanneganti, T. -D., Kanno, H., Kanthasamy, A. G., Kantorow, M., Kaparakis-Liaskos, M., Kapuy, O., Karantza, V., Karim, M. R., Karmakar, P., Kaser, A., Kaushik, S., Kawula, T., Kaynar, A. M., P. -Y., Ke, Z. -J., Ke, Kehrl, J. H., Keller, K. E., Kemper, J. K., Kenworthy, A. K., Kepp, O., Kern, A., Kesari, S., Kessel, D., Ketteler, R., Kettelhut, I. C., Khambu, B., Khan, M. M., Khandelwal, V. K. M., Khare, S., Kiang, J. G., Kiger, A. A., Kihara, A., Kim, A. L., Kim, C. H., Kim, D. R., Kim, D. -H., Kim, E. K., Kim, H. Y., Kim, H. -R., Kim, J. -S., Kim, J. H., Kim, J. C., Kim, K. W., Kim, M. D., Kim, M. -M., Kim, P. K., Kim, S. W., Kim, S. -Y., Kim, Y. -S., Kim, Y., Kimchi, A., Kimmelman, A. C., Kimura, T., King, J. S., Kirkegaard, K., Kirkin, V., Kirshenbaum, L. A., Kishi, S., Kitajima, Y., Kitamoto, K., Kitaoka, Y., Kitazato, K., Kley, R. A., Klimecki, W. 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Murat, Ke, Po-Yuan, Ke, Zun-Ji, Kehrl, John H., Keller, Kate E., Kemper, Jongsook Kim, Kenworthy, Anne K., Kepp, Oliver, Kern, Andrea, Kesari, Santosh, Kessel, David, Ketteler, Robin, Kettelhut, Isis do Carmo, Khambu, Bilon, Khan, Muzamil Majid, Khandelwal, Vinoth K.M., Khare, Sangeeta, Kiang, Juliann G., Kiger, Amy A., Kihara, Akio, Kim, Arianna L., Kim, Cheol Hyeon, Kim, Deok Ryong, Kim, Do-Hyung, Kim, Eung Kweon, Kim, Hye Young, Kim, Hyung-Ryong, Kim, Jae-Sung, Kim, Jeong Hun, Kim, Jin Cheon, Kim, Jin Hyoung, Kim, Kwang Woon, Kim, Michael D., Kim, Moon-Moo, Kim, Peter K., Kim, Seong Who, Kim, Soo-Youl, Kim, Yong-Sun, Kim, Yonghyun, Kimchi, Adi, Kimmelman, Alec C., Kimura, Tomonori, King, Jason S., Kirkegaard, Karla, Kirkin, Vladimir, Kirshenbaum, Lorrie A., Kishi, Shuji, Kitajima, Yasuo, Kitamoto, Katsuhiko, Kitaoka, Yasushi, Kitazato, Kaio, Kley, Rudolf A., Klimecki, Walter T., Klinkenberg, Michael, Klucken, Jochen, Knævelsrud, Helene, Knecht, Erwin, Knuppertz, Laura, Ko, Jiunn-Liang, Kobayashi, Satoru, Koch, Jan C., Koechlin-Ramonatxo, Christelle, Koenig, Ulrich, Koh, Young Ho, Köhler, Katja, Kohlwein, Sepp D., Koike, Masato, Komatsu, Masaaki, Kominami, Eiki, Kong, Dexin, Kong, Hee Jeong, Konstantakou, Eumorphia G., Kopp, Benjamin T., Korcsmaros, Tama, Korhonen, Laura, Korolchuk, Viktor I., Koshkina, Nadya V., Kou, Yanjun, Koukourakis, Michael I., Koumenis, Constantino, Kovács, Attila L., Kovács, Tibor, Kovacs, Werner J., Koya, Daisuke, Kraft, Claudine, Krainc, Dimitri, Kramer, Helmut, Kravic-Stevovic, Tamara, Krek, Wilhelm, Kretz-Remy, Carole, Krick, Roswitha, Krishnamurthy, Malathi, Kriston-Vizi, Jano, Kroemer, Guido, Kruer, Michael C., Kruger, Rejko, Ktistakis, Nicholas T., Kuchitsu, Kazuyuki, Kuhn, Christian, Kumar, Addanki Pratap, Kumar, Anuj, Kumar, Ashok, Kumar, Deepak, Kumar, Dhiraj, Kumar, Rakesh, Kumar, Sharad, Kundu, Mondira, Kung, Hsing-Jien, Kuno, Atsushi, Kuo, Sheng-Han, Kuret, Jeff, Kurz, Tino, Kwok, Terry, Kwon, Taeg Kyu, Kwon, Yong Tae, Kyrmizi, Irene, La Spada, Albert R., Lafont, Frank, Lahm, Tim, Lakkaraju, Aparna, Lam, Truong, Lamark, Trond, Lancel, Steve, Landowski, Terry H., Lane, Darius J.R., Lane, Jon D., Lanzi, Cinzia, Lapaquette, Pierre, Lapierre, Louis R., Laporte, Jocelyn, Laukkarinen, Johanna, Laurie, Gordon W., Lavandero, Sergio, Lavie, Lena, Lavoie, Matthew J., Law, Betty Yuen Kwan, Law, Helen Ka-Wai, Law, Kelsey B., Layfield, Robert, Lazo, Pedro A., Le Cam, Laurent, Le Roch, Karine G., Le Stunff, Hervé, Leardkamolkarn, Vijittra, Lecuit, Marc, Lee, Byung-Hoon, Lee, Che-Hsin, Lee, Erinna F., Lee, Gyun Min, Lee, He-Jin, Lee, Hsinyu, Lee, Jae Keun, Lee, Jongdae, Lee, Ju-Hyun, Lee, Jun Hee, Lee, Michael, Lee, Myung-Shik, Lee, Patty J., Lee, Sam W., Lee, Seung-Jae, Lee, Shiow-Ju, Lee, Stella Y., Lee, Sug Hyung, Lee, Sung Sik, Lee, Sung-Joon, Lee, Sunhee, Lee, Ying-Ray, Lee, Yong J., Lee, Young H., Leeuwenburgh, Christiaan, Lefort, Sylvain, Legouis, Renaud, Lei, Jinzhi, Lei, Qun-Ying, Leib, David A., Leibowitz, Gil, Lekli, Istvan, Lemaire, Stéphane D., Lemasters, John J., Lemberg, Marius K., Lemoine, Antoinette, Leng, Shuilong, Lenz, Guido, Lenzi, Paola, Lerman, Lilach O., Barbato, Daniele Lettieri, Leu, Julia I. 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Sue, Menna-Barreto, Rubem F.S., Menon, Manoj B., Meraz-Ríos, Marco A., Merla, Giuseppe, Merlini, Luciano, Merlot, Angelica M., Meryk, Andrea, Meschini, Stefania, Meyer, Joel N., Mi, Man-Tian, Miao, Chao-Yu, Micale, Lucia, Michaeli, Simon, Michiels, Carine, Migliaccio, Anna Rita, Mihailidou, Anastasia Susie, Mijaljica, Dalibor, Mikoshiba, Katsuhiko, Milan, Enrico, Miller-Fleming, Leonor, Mills, Gordon B., Mills, Ian G., Minakaki, Georgia, Minassian, Berge A., Ming, Xiu-Fen, Minibayeva, Farida, Minina, Elena A., Mintern, Justine D., Minucci, Saverio, Miranda-Vizuete, Antonio, Mitchell, Claire H., Miyamoto, Shigeki, Miyazawa, Keisuke, Mizushima, Noboru, Mnich, Katarzyna, Mograbi, Baharia, Mohseni, Simin, Moita, Luis Ferreira, Molinari, Marco, Molinari, Maurizio, Møller, Andreas Buch, Mollereau, Bertrand, Mollinedo, Faustino, Mongillo, Marco, Monick, Martha M., Montagnaro, Serena, Montell, Craig, Moore, Darren J., Moore, Michael N., Mora-Rodriguez, Rodrigo, Moreira, Paula I., Morel, Etienne, Morelli, Maria Beatrice, Moreno, Sandra, Morgan, Michael J., Moris, Arnaud, Moriyasu, Yuji, Morrison, Janna L., Morrison, Lynda A., Morselli, Eugenia, Moscat, Jorge, Moseley, Pope L., Mostowy, Serge, Motori, Elisa, Mottet, Deni, Mottram, Jeremy C., Moussa, Charbel E.-H., Mpakou, Vassiliki E., Mukhtar, Hasan, Levy, Jean M. Mulcahy, Muller, Sylviane, Muñoz-Moreno, Raquel, Muñoz-Pinedo, Cristina, Münz, Christian, Murphy, Maureen E., Murray, James T., Murthy, Aditya, Mysorekar, Indira U., Nabi, Ivan R., Nabissi, Massimo, Nader, Gustavo A., Nagahara, Yukitoshi, Nagai, Yoshitaka, Nagata, Kazuhiro, Nagelkerke, Anika, Nagy, Péter, Naidu, Samisubbu R., Nair, Sreejayan, Nakano, Hiroyasu, Nakatogawa, Hitoshi, Nanjundan, Meera, Napolitano, Gennaro, Naqvi, Naweed I., Nardacci, Roberta, Narendra, Derek P., Narita, Masashi, Nascimbeni, Anna Chiara, Natarajan, Ramesh, Navegantes, Luiz C., Nawrocki, Steffan T., Nazarko, Taras Y., Nazarko, Volodymyr Y., Neill, Thoma, Neri, Luca M., Netea, Mihai G., Netea-Maier, Romana T., Neves, Bruno M., Ney, Paul A., Nezis, Ioannis P., Nguyen, Hang T.T., Nguyen, Huu Phuc, Nicot, Anne-Sophie, Nilsen, Hilde, Nilsson, Per, Nishimura, Mikio, Nishino, Ichizo, Niso-Santano, Mireia, Niu, Hua, Nixon, Ralph A., Njar, Vincent C.O., Noda, Takeshi, Noegel, Angelika A., Nolte, Elsie Magdalena, Norberg, Erik, Norga, Koenraad K., Noureini, Sakineh Kazemi, Notomi, Shoji, Notterpek, Lucia, Nowikovsky, Karin, Nukina, Nobuyuki, Nürnberger, Thorsten, O'donnell, Valerie B., O'donovan, Tracey, O'dwyer, Peter J., Oehme, Ina, Oeste, Clara L., Ogawa, Michinaga, Ogretmen, Besim, Ogura, Yuji, Oh, Young J., Ohmuraya, Masaki, Ohshima, Takayuki, Ojha, Rani, Okamoto, Koji, Okazaki, Toshiro, Oliver, F. Javier, Ollinger, Karin, Olsson, Stefan, Orban, Daniel P., Ordonez, Paulina, Orhon, Idil, Orosz, Laszlo, O'rourke, Eyleen J., Orozco, Helena, Ortega, Angel L., Ortona, Elena, Osellame, Laura D., Oshima, Junko, Oshima, Shigeru, Osiewacz, Heinz D., Otomo, Takanobu, Otsu, Kinya, Ou, Jing-Hsiung Jame, Outeiro, Tiago F., Ouyang, Dong-Yun, Ouyang, Hongjiao, Overholtzer, Michael, Ozbun, Michelle A., Ozdinler, P. Hande, Ozpolat, Bulent, Pacelli, Consiglia, Paganetti, Paolo, Page, Guyléne, Pages, Gille, Pagnini, Ugo, Pajak, Beata, Pak, Stephen C., Pakos-Zebrucka, Karolina, Pakpour, Nazzy, Palková, Zdena, Palladino, Francesca, Pallauf, Kathrin, Pallet, Nicola, Palmieri, Marta, Paludan, Søren R., Palumbo, Camilla, Palumbo, Silvia, Pampliega, Olatz, Pan, Hongming, Pan, Wei, Panaretakis, Theochari, Pandey, Aseem, Pantazopoulou, Areti, Papackova, Zuzana, Papademetrio, Daniela L., Papassideri, Issidora, Papini, Alessio, Parajuli, Nirmala, Pardo, Julian, Parekh, Vrajesh V., Parenti, Giancarlo, Park, Jong-In, Park, Junsoo, Park, Ohkmae K., Parker, Roy, Parlato, Rosanna, Parys, Jan B., Parzych, Katherine R., Pasquet, Jean-Max, Pasquier, Benoit, Pasumarthi, Kishore B.S., Patschan, Daniel, Pattingre, Sophie, Pattison, Scott, Pause, Arnim, Pavenstädt, Hermann, Pavone, Flaminia, Pedrozo, Zully, Peña, Fernando J., Peñalva, Miguel A., Pende, Mario, Peng, Jianxin, Penna, Fabio, Penninger, Josef M., Pensalfini, Anna, Pepe, Salvatore, Pereira, Gustavo J.S., Pereira, Paulo C., de la Cruz, Verónica Pérez, Pérez-Pérez, María Esther, Pérez-Rodríguez, Diego, Pérez-Sala, Dolore, Perier, Celine, Perl, Andra, Perlmutter, David H., Perrotta, Ida, Pervaiz, Shazib, Pesonen, Maija, Pessin, Jeffrey E., Peters, Godefridus J., Petersen, Morten, Petrache, Irina, Petrof, Basil J., Petrovski, Goran, Phang, James M., Piacentini, Mauro, Pierdominici, Marina, Pierre, Philippe, Pierrefite-Carle, Valérie, Pietrocola, Federico, Pimentel-Muiños, Felipe X., Pinar, Mario, Pineda, Benjamin, Pinkas-Kramarski, Ronit, Pinti, Marcello, Pinton, Paolo, Piperdi, Bilal, Piret, James M., Platanias, Leonidas C., Platta, Harald W., Plowey, Edward D., Pöggeler, Stefanie, Poirot, Marc, Polčic, Peter, Poletti, Angelo, Poon, Audrey H., Popelka, Hana, Popova, Blagovesta, Poprawa, Izabela, Poulose, Shibu M., Poulton, Joanna, Powers, Scott K., Powers, Ted, Pozuelo-Rubio, Mercede, Prak, Krisna, Prange, Reinhild, Prescott, Mark, Priault, Muriel, Prince, Sharon, Proia, Richard L., Proikas-Cezanne, Tassula, Prokisch, Holger, Promponas, Vasilis J., Przyklenk, Karin, Puertollano, Rosa, Pugazhenthi, Subbiah, Puglielli, Luigi, Pujol, Aurora, Puyal, Julien, Pyeon, Dohun, Qi, Xin, Qian, Wen-Bin, Qin, Zheng-Hong, Qiu, Yu, Qu, Ziwei, Quadrilatero, Joe, Quinn, Frederick, Raben, Nina, Rabinowich, Hannah, Radogna, Flavia, Ragusa, Michael J., Rahmani, Mohamed, Raina, Komal, Ramanadham, Sasanka, Ramesh, Rajagopal, Rami, Abdelhaq, Randall-Demllo, Sarron, Randow, Felix, Rao, Hai, Rao, V. Ashutosh, Rasmussen, Blake B., Rasse, Tobias M., Ratovitski, Edward A., Rautou, Pierre-Emmanuel, Ray, Swapan K., Razani, Babak, Reed, Bruce H., Reggiori, Fulvio, Rehm, Marku, Reichert, Andreas S., Rein, Theo, Reiner, David J., Reits, Eric, Ren, Jun, Ren, Xingcong, Renna, Maurizio, Reusch, Jane E.B., Revuelta, Jose L., Reyes, Leticia, Rezaie, Alireza R., Richards, Robert I., Richardson, Des R., Richetta, Clémence, Riehle, Michael A., Rihn, Bertrand H., Rikihisa, Yasuko, Riley, Brigit E., Rimbach, Gerald, Rippo, Maria Rita, Ritis, Konstantino, Rizzi, Federica, Rizzo, Elizete, Roach, Peter J., Robbins, Jeffrey, Roberge, Michel, Roca, Gabriela, Roccheri, Maria Carmela, Rocha, Sonia, Rodrigues, Cecilia M.P., Rodríguez, Clara I., de Cordoba, Santiago Rodriguez, Rodriguez-Muela, Natalia, Roelofs, Jeroen, Rogov, Vladimir V., Rohn, Troy T., Rohrer, Bärbel, Romanelli, Davide, Romani, Luigina, Romano, Patricia Silvia, Roncero, M. 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Haung, Yu, Xiao-Fang, Yu, Zhengping, Yuan, Junying, Yuan, Zhi-Min, Yue, Beatrice Y.J.T., Yue, Jianbo, Yue, Zhenyu, Zacks, David N., Zacksenhaus, Eldad, Zaffaroni, Nadia, Zaglia, Tania, Zakeri, Zahra, Zecchini, Vincent, Zeng, Jinsheng, Zeng, Min, Zeng, Qi, Zervos, Antonis S., Zhang, Donna D., Zhang, Fan, Zhang, Guo, Zhang, Guo-Chang, Zhang, Hao, Zhang, Hong, Zhang, Hongbing, Zhang, Jian, Zhang, Jiangwei, Zhang, Jianhua, Zhang, Jing-Pu, Zhang, Li, Zhang, Lin, Zhang, Long, Zhang, Ming-Yong, Zhang, Xiangnan, Zhang, Xu Dong, Zhang, Yan, Zhang, Yang, Zhang, Yanjin, Zhang, Yingmei, Zhang, Yunjiao, Zhao, Mei, Zhao, Wei-Li, Zhao, Xiaonan, Zhao, Yan G., Zhao, Ying, Zhao, Yongchao, Zhao, Yu-Xia, Zhao, Zhendong, Zhao, Zhizhuang J., Zheng, Dexian, Zheng, Xi-Long, Zheng, Xiaoxiang, Zhivotovsky, Bori, Zhong, Qing, Zhou, Guang-Zhou, Zhou, Guofei, Zhou, Huiping, Zhou, Shu-Feng, Zhou, Xu-Jie, Zhu, Hongxin, Zhu, Hua, Zhu, Wei-Guo, Zhu, Wenhua, Zhu, Xiao-Feng, Zhu, Yuhua, Zhuang, Shi-Mei, Zhuang, Xiaohong, Ziparo, Elio, Zois, Christos E., Zoladek, Teresa, Zong, Wei-Xing, Zorzano, Antonio, and Zughaier, Susu M.

Subjects
Molecular Biology, Cell Biology, Settore BIO/06 - Anatomia Comparata E Citologia
Abstract

non presente

Published
2016

38. Entrance order and side preference of buffalo cows in the milking parlour

Author

DE ROSA, GIUSEPPE, GRASSO, FERNANDO, POLIKARPUS A., PACELLI C., NAPOLITANO F., Susanne Waiblinger, Christoph Winckler, Anke Gutmann, DE ROSA, Giuseppe, Polikarpus, A., Grasso, Fernando, Pacelli, C., and Napolitano, F.

Subjects
Lactating buffaloe, Entrance order, Side preference, Milking parlour
Abstract

The study was conducted to investigate the consistency of the entrance order into the milking parlour of dairy buffalo and the relationship between milking order and milk yield. In addition, the preference for a determinate side of the milking parlour has been considered. Fifty-seven primiparous buffalo cows were used. Before milking whole group entered into the waiting area and they were free to choose their position in relation to the entrance in the milking parlour, without any intervention of the stockman. Both sides of the parlour were identical and cows had access to each side of the parlour through pneumatically operated gates. They were milked in a 5 x 5 auto-tandem milking parlour. For each cow, entrance order into milking parlour, side where she was milked and milk yield were recorded. These data were derived from computerised cows’ identification.. A constancy of the entrance order into milking parlour was found. More productive cows tended to enter the milking parlour first. Twenty-seven cows (47.4%) preferred the right side of the milking parlour, 25 the left side (43.8%) and the remaining 5 (8.8%) showed no preference. Forty-three out of 57 animals were milked in 99-100% occasions in the same side. Nine of the animals showing a side preference also exhibited a stall preference. Our results showed that buffaloes can present marked entrance order consistency and side preference.

Published
2012

39. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).

Author

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Sardiello, M, Sargent, G, Sarkar, P, Sarkar, S, Sarrias, Mr, Sarwal, Mm, Sasakawa, C, Sasaki, M, Sass, M, Sato, K, Sato, M, Satriano, J, Savaraj, N, Saveljeva, S, Schaefer, L, Schaible, Ue, Scharl, M, Schatzl, Hm, Schekman, R, Scheper, W, Schiavi, A, Schipper, Hm, Schmeisser, H, Schmidt, J, Schmitz, I, Schneider, Be, Schneider, Em, Schneider, Jl, Schon, Ea, Schönenberger, Mj, Schönthal, Ah, Schorderet, Df, Schröder, B, Schuck, S, Schulze, Rj, Schwarten, M, Schwarz, Tl, Sciarretta, S, Scotto, K, Scovassi, Ai, Screaton, Ra, Screen, M, Seca, H, Sedej, S, Segatori, L, Segev, N, Seglen, Po, Seguí-Simarro, Jm, Segura-Aguilar, J, Seki, E, Sell, C, Seiliez, I, Semenkovich, Cf, Semenza, Gl, Sen, U, Serra, Al, Serrano-Puebla, A, Sesaki, H, Setoguchi, T, Settembre, C, Shacka, Jj, Shajahan-Haq, An, Shapiro, Im, Sharma, S, She, H, Shen, Ck, Shen, Cc, Shen, Hm, Shen, S, Shen, W, Sheng, R, Sheng, X, Sheng, Zh, Shepherd, Tg, Shi, J, Shi, Q, Shi, Y, Shibutani, S, Shibuya, K, Shidoji, Y, Shieh, Jj, Shih, Cm, Shimada, Y, Shimizu, S, Shin, Dw, Shinohara, Ml, Shintani, M, Shintani, T, Shioi, T, Shirabe, K, Shiri-Sverdlov, R, Shirihai, O, Shore, Gc, Shu, Cw, Shukla, D, Sibirny, Aa, Sica, V, Sigurdson, Cj, Sigurdsson, Em, Sijwali, P, Sikorska, B, Silveira, Wa, Silvente-Poirot, S, Silverman, Ga, Simak, J, Simmet, T, Simon, Ak, Simon, Hu, Simone, C, Simons, M, Simonsen, A, Singh, R, Singh, Sv, Singh, Sk, Sinha, D, Sinha, S, Sinicrope, Fa, Sirko, A, Sirohi, K, Sishi, Bj, Sittler, A, Siu, Pm, Sivridis, E, Skwarska, A, Slack, R, Slaninová, I, Slavov, N, Smaili, S, Smalley, K, Smith, Dr, Soenen, Sj, Soleimanpour, Sa, Solhaug, A, Somasundaram, K, Son, Jh, Sonawane, A, Song, C, Song, F, Song, Hk, Song, Jx, Song, W, Soo, Ky, Sood, Ak, Soong, Tw, Soontornniyomkij, V, Sorice, M, Sotgia, F, Soto-Pantoja, Dr, Sotthibundhu, A, Sousa, Mj, Spaink, Hp, Span, Pn, Spang, A, Sparks, Jd, Speck, Pg, Spector, Sa, Spies, Cd, Springer, W, Clair, D, Stacchiotti, A, Staels, B, Stang, Mt, Starczynowski, Dt, Starokadomskyy, P, Steegborn, C, Steele, Jw, Stefanis, L, Steffan, J, Stellrecht, Cm, Stenmark, H, Stepkowski, Tm, Stern, St, Stevens, C, Stockwell, Br, Stoka, V, Storchova, Z, Stork, B, Stratoulias, V, Stravopodis, Dj, Strnad, P, Strohecker, Am, Ström, Al, Stromhaug, P, Stulik, J, Su, Yx, Su, Z, Subauste, C, Subramaniam, S, Sue, Cm, Suh, Sw, Sui, X, Sukseree, S, Sulzer, D, Sun, Fl, Sun, J, Sun, Sy, Sun, Y, Sundaramoorthy, V, Sung, J, Suzuki, H, Suzuki, K, Suzuki, N, Suzuki, T, Suzuki, Yj, Swanson, M, Swanton, C, Swärd, K, Swarup, G, Sweeney, St, Sylvester, Pw, Szatmari, Z, Szegezdi, E, Szlosarek, Pw, Taegtmeyer, H, Tafani, M, Taillebourg, E, Tait, Sw, Takacs-Vellai, K, Takahashi, Y, Takáts, S, Takemura, G, Takigawa, N, Talbot, Nj, Tamagno, E, Tamburini, J, Tan, Cp, Tan, L, Tan, Ml, Tan, M, Tan, Yj, Tanaka, K, Tanaka, M, Tang, D, Tang, G, Tanida, I, Tanji, K, Tannous, Ba, Tapia, Ja, Tasset-Cuevas, I, Tatar, M, Tavassoly, I, Tavernarakis, N, Taylor, A, Taylor, G, Taylor, Ga, Taylor, Jp, Taylor, Mj, Tchetina, Ev, Tee, Ar, Teixeira-Clerc, F, Telang, S, Tencomnao, T, Teng, Bb, Teng, Rj, Terro, F, Tettamanti, G, Theiss, Al, Theron, Ae, Thomas, Kj, Thomé, Mp, Thomes, Pg, Thorburn, A, Thorner, J, Thum, T, Thumm, M, Thurston, Tl, Tian, L, Till, A, Ting, Jp, Titorenko, Vi, Toker, L, Toldo, S, Tooze, Sa, Topisirovic, I, Torgersen, Ml, Torosantucci, L, Torriglia, A, Torrisi, Mr, Tournier, C, Towns, R, Trajkovic, V, Travassos, Lh, Triola, G, Tripathi, Dn, Trisciuoglio, D, Troncoso, R, Trougakos, Ip, Truttmann, Ac, Tsai, Kj, Tschan, Mp, Tseng, Yh, Tsukuba, T, Tsung, A, Tsvetkov, A, Tu, S, Tuan, Hy, Tucci, M, Tumbarello, Da, Turk, B, Turk, V, Turner, Rf, Tveita, Aa, Tyagi, Sc, Ubukata, M, Uchiyama, Y, Udelnow, A, Ueno, T, Umekawa, M, Umemiya-Shirafuji, R, Underwood, Br, Ungermann, C, Ureshino, Rp, Ushioda, R, Uversky, Vn, Uzcátegui, Nl, Vaccari, T, Vaccaro, Mi, Váchová, L, Vakifahmetoglu-Norberg, H, Valdor, R, Valente, Em, Vallette, F, Valverde, Am, Van den Berghe, G, Van Den Bosch, L, van den Brink, Gr, van der Goot, Fg, van der Klei, Ij, van der Laan, Lj, van Doorn, Wg, van Egmond, M, van Golen, Kl, Van Kaer, L, van Lookeren Campagne, M, Vandenabeele, P, Vandenberghe, W, Vanhorebeek, I, Varela-Nieto, I, Vasconcelos, Mh, Vasko, R, Vavvas, Dg, Vega-Naredo, I, Velasco, G, Velentzas, Ad, Velentzas, Pd, Vellai, T, Vellenga, E, Vendelbo, Mh, Venkatachalam, K, Ventura, N, Ventura, S, Veras, P, Verdier, M, Vertessy, Bg, Viale, A, Vidal, M, Vieira, Hl, Vierstra, Rd, Vigneswaran, N, Vij, N, Vila, M, Villar, M, Villar, Vh, Villarroya, J, Vindis, C, Viola, G, Viscomi, Maria Teresa, Vitale, G, Vogl, Dt, Voitsekhovskaja, Ov, von Haefen, C, von Schwarzenberg, K, Voth, De, Vouret-Craviari, V, Vuori, K, Vyas, Jm, Waeber, C, Walker, Cl, Walker, Mj, Walter, J, Wan, L, Wan, X, Wang, B, Wang, C, Wang, Cy, Wang, D, Wang, F, Wang, G, Wang, Hj, Wang, H, Wang, Hg, Wang, Hd, Wang, J, Wang, M, Wang, Mq, Wang, Py, Wang, P, Wang, Rc, Wang, S, Wang, Tf, Wang, X, Wang, Xj, Wang, Xw, Wang, Y, Wang, Yj, Wang, Yt, Wang, Zn, Wappner, P, Ward, C, Ward, Dm, Warnes, G, Watada, H, Watanabe, Y, Watase, K, Weaver, Te, Weekes, Cd, Wei, J, Weide, T, Weihl, Cc, Weindl, G, Weis, Sn, Wen, L, Wen, X, Wen, Y, Westermann, B, Weyand, Cm, White, Ar, White, E, Whitton, Jl, Whitworth, Aj, Wiels, J, Wild, F, Wildenberg, Me, Wileman, T, Wilkinson, D, Wilkinson, S, Willbold, D, Williams, C, Williams, K, Williamson, Pr, Winklhofer, Kf, Witkin, S, Wohlgemuth, Se, Wollert, T, Wolvetang, Ej, Wong, E, Wong, Gw, Wong, Rw, Wong, Vk, Woodcock, Ea, Wright, Kl, Wu, C, Wu, D, Wu, G, Wu, J, Wu, M, Wu, S, Wu, Wk, Wu, Y, Wu, Z, Xavier, Cp, Xavier, Rj, Xia, Gx, Xia, T, Xia, W, Xia, Y, Xiao, H, Xiao, J, Xiao, S, Xiao, W, Xie, Cm, Xie, Z, Xilouri, M, Xiong, Y, Xu, C, Xu, F, Xu, H, Xu, J, Xu, L, X, Xu, Xu, Y, Xu, Zx, Xu, Z, Xue, Y, Yamada, T, Yamamoto, A, Yamanaka, K, Yamashina, S, Yamashiro, S, Yan, B, Yan, X, Yan, Z, Yanagi, Y, Yang, D, Yang, Jm, Yang, L, Yang, M, Yang, Pm, Yang, P, Yang, Q, Yang, W, Yang, Wy, Yang, X, Yang, Y, Yang, Z, Yao, Mc, Yao, Pj, Yao, X, Yao, Z, Yasui, L, Ye, M, Yedvobnick, B, Yeganeh, B, Yeh, E, Yeyati, Pl, Yi, F, Yi, L, Yin, Xm, Yip, Ck, Yoo, Ym, Yoo, Yh, Yoon, Sy, Yoshida, K, Yoshimori, T, Young, Kh, Yu, H, Yu, Jj, Yu, Jt, Yu, J, Yu, L, Yu, Wh, Yu, Xf, Yu, Z, Yuan, J, Yuan, Zm, Yue, By, Yue, J, Yue, Z, Zacks, Dn, Zacksenhaus, E, Zaffaroni, N, Zaglia, T, Zakeri, Z, Zecchini, V, Zeng, J, Zeng, M, Zeng, Q, Zervos, A, Zhang, Dd, Zhang, F, Zhang, G, Zhang, Gc, Zhang, H, Zhang, J, Zhang, Jp, Zhang, L, Zhang, My, Zhang, X, Zhang, Xd, Zhang, Y, Zhao, M, Zhao, Wl, Zhao, X, Zhao, Yg, Zhao, Y, Zhao, Yx, Zhao, Z, Zhao, Zj, Zheng, D, Zheng, Xl, Zheng, X, Zhivotovsky, B, Zhong, Q, Zhou, Gz, Zhou, G, Zhou, H, Zhou, Sf, Zhou, Xj, Zhu, H, Zhu, Wg, Zhu, W, Zhu, Xf, Zhu, Y, Zhuang, Sm, Zhuang, X, Ziparo, E, Zois, Ce, Zoladek, T, Zong, Wx, Zorzano, A, Zughaier, Sm., Giordano A (ORCID:0000-0002-6978-0880), and Viscomi MT (ORCID:0000-0002-9096-4967)

Abstract

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure flux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we prese

Published
2016

40. Corpus luteum function and pregnancy outcome in buffaloes during the transition period from breeding to non-breeding season

Author

RUSSO, MARCO, VECCHIO, DOMENICO, NEGLIA, GIANLUCA, GASPARRINI, BIANCA, ZICARELLI, LUIGI, CAMPANILE, GIUSEPPE, Pacelli C., Prandi A., D’Occhio M. J., Russo, Marco, Vecchio, Domenico, Neglia, Gianluca, Pacelli, C., Prandi, A., Gasparrini, Bianca, Zicarelli, Luigi, D’Occhio, M. J., and Campanile, Giuseppe

Subjects
GNRH AGONIST, COWS, LOCAL CHANGES, ESTROUS-CYCLE, PROSTAGLANDIN-F2-ALPHA, UTERINE BLOOD-FLOW, SYNCHRONIZATION, EMBRYONIC MORTALITY, TRANSRECTAL DOPPLER SONOGRAPHY, PROGESTERONE LEVELS
Abstract

The aim in this study was to investigate corpus luteum function and embryonic loss in buffaloes mated by artificial inseminations (AI) during the transitional period from breeding to non-breeding season. The study was carried out using 288 multiparous Italian Mediterranean Buffalo cows at 110 ± 4 days in milk. The buffaloes were mated by AI after synchronization of ovulation by the Ovsynch-TAI protocol 25 days after AI buffaloes underwent trans-rectal ultrasonography to assess embryonic development. Pregnancy diagnosis was confirmed on Days 45 and 70 after AI by rectal palpation. Buffaloes pregnant on Day 25 but not on Day 45 were considered to have undergone late embryonic mortality (LEM), whilst buffaloes pregnant on Day 45 but not on Day 70 were considered to have undergone foetal mortality (FM). Corpus luteum size and blood flow were determined by real-time B-mode/colour-Doppler on day 10 after AI in 122 buffaloes. The resistive index (RI) and pulsatility index (PI) were recorded at the time. Milk samples were collected on Days 10, 20 and 25 after AI in all inseminated buffaloes for the assay of whey P4 concentrations. Data were analysed by anova. Pregnancy rate on Day 25 after AI was 48.6% (140/288) and declined to 35.4% (102/288) and 30.6% (88/288) by Day 45 and Day 70 respectively. The incidences of LEM and FM were respectively 27.1% (38/140) and 13.7% (14/102). Pregnant buffaloes had greater (p

Published
2010

41. Effect of space availability on productive and reproductive performances in buffalo cows

Author

VECCHIO, DOMENICO, CAMPANILE, GIUSEPPE, Zicarelli G., Pacelli C., Zicarelli F., Vecchio, Domenico, Zicarelli, G., Pacelli, C., Zicarelli, F., and Campanile, Giuseppe

Subjects
Milk production, Space availability, Buffalo
Abstract

The effects of space availability on productive and reproductive performances have been evaluated in buffalo cows. The trial was carried out on 124 lactating buffaloes (69±84 days in milk) equally divided in two experimental groups (group 1 and group 2) with different space availability (15 vs 20m2/ head). Daily milk production was recorded in each group and a sample of milk was monthly analysed for fat and protein contents. Furthermore, fertility and intercalving and start trial-conception (number of days from the beginning of the trial until conception) intervals were also evaluated. Statistical analysis was performed by ANOVA with repeated measures for productive parameters and by Student’s t Test for intercalving and start trial-conception intervals. Buffaloes in Group 2 showed higher (P

Published
2009

42. Effetto di una dieta a base di insilato di erba sulla qualità del latte di bufala

Author

Tripaldi C., Pacelli C., Palocci G., Mazzi M., Borghese A., NEGLIA, GIANLUCA, Tripaldi, C., Neglia, Gianluca, Pacelli, C., Palocci, G., Mazzi, M., and Borghese, A.

Subjects
Qualità del latte, Insilato di loietto italico, Bufalo
Abstract

The aim of this trial is to evaluate the effect of a diet based on silage of Italian Ryegrass on the yield and quality of buffalo milk. The trial was carried out in a commercial farm located in South of Italy, on 40 animals divided in two groups homogeneous for parity, lactation stage and milk yield. Silage group (n = 20) received a total mixed ration based on silage of Italian Ryegrass, hay and concentrates, whereas hay group (n = 20) was fed by hay and concentrates. Both diets contained 0.92 Milk Forage Units/kg d.m. and 15.5% crude protein/d.m. Milk recordings were monthly carried out during morning milking in order to evaluate the milk yield, pH, fat, protein, somatic cell count and coagulating properties. Statistical analysis was performed using a factorial model. A higher milk yield was observed in silage group respect to hay group in the total period of the trial (5.7 vs. 5.1 kg; P ≤ 0.01), while no significant differences were recorded in fat and protein content (7.78 vs. 7.53% and 4.33 vs. 4.35%, respectively in silage and hay group). Also pH and somatic cell count were not different between the groups. The milk from silage group showed better coagulating properties than those of the other group: the clotting time decreased (18.3 vs. 19.4 min; P≤0.01) and the curd firmness increased (45.2 vs. 39.6 mm e 49.9 vs. 45.2 mm; P ≤ 0,01). From the results of this trial it comes out that the diet based on silage of Italian Ryegrass affects positively the yield of buffalo milk and its coagulating properties.

Published
2009

43. Influence of weaning age on growth dynamics of young buffalo bulls until 90 days of age

Author

Monica Isabella Cutrignelli, Bovera, F., Marchiello, M., Di Lella, T., Pacelli, C., Cutrignelli, MONICA ISABELLA, Bovera, Fulvia, M., Marchiello, T., DI LELLA, and C., Pacelli

Subjects
animal diseases, food and beverages, young buffalo bulls, early weaning, Biological Efficiency of Growth, lcsh:Animal culture, lcsh:SF1-1100
Abstract

In recent years, measures have been taken to promote buffalo meat consumption, with a fair degree of success. Nevertheless, the majority of young male bulls are still sacrificed shortly after birth due to high meat production costs...

Published
2011

44. BIOMEX (Biology and Mars Experiment): Preliminary results on Antarctic black cryptoendolithic fungi in ground based experiments

Author

Pacelli, C., Selbmann, L., Onofri, S., and de Vera, Jean Pierre Paul

Subjects
BIOMEX, space experiment, Mars, Experimentelle Planetenphysik, antarctic black fungi, simulation
Abstract

The main goal for astrobiologists is to find traces of present or past life in extraterrestrial environment or in meteorites. Biomolecules, such as lipids, pigments or polysaccharides, may be useful to establish the presence of extant or extinct life (Simoneit, B et al., 1998). BIOMEX (Biology and Mars Experiment) aims to measure to what extent biomolecules, such as pigments and cellular components, preserve their stability under space and Mars-like conditions. The experiment has just been launched in the space and will be exposed on EXPOSE-R payload to the outside of the International Space Station (ISS) for about 2 years. Among a number of extremophilic microorganisms tested, the Antarctic cryptoendolithic black fungus Cryomyces antarcticus CCFEE 515 was included in the experiment. The fungus, living in the airspaces of porous rocks, was already chosen in previous astrobiological investigation for studying the interplanetary transfer of life via meteorites. In that context, the fungus survived 18 months of exposure outside of the ISS (Onofri al., 2012); for all these reasons it is considered an optimal eukaryotic model for astrobiological exploration. Before launch dried samples were exposed, in ground based experiments, to extreme conditions, including vacuum, irradiation and temperature cycles.Upon sample re-hydration and survival analysis, including colony forming ability, Propidium MonoAzide (PMA) assay-coupled quantitative PCR (Mohapatra and La Duc, 2012) all the test systems survived, neither any DNA damage was detectable. Our analyses focused also on mineral-microorganisms interactions and stability/degradation of typical fungal macromolecules, in particular melanin, when exposed to space and simulated Martian conditions, contributing to the development of libraries of biosignatures in rocks, supporting future exploration missions.

Published
2014

45. BIOMEX: Three different steps to approach a systematic determination of habitats and stable biosignatures in space- and Mars-like environments

Author

de Vera, Jean Pierre Paul, Böttger, Ute, Lorek, Andreas, Wolter, D., Hübers, Heinz-Wilhelm, de la Torre Noetzel, R., Sánchez Iñigo, F.J., Billi, D., Baqué, M., Verseux, C., Rettberg, Petra, Rabbow, Elke, Panitz, C., Reitz, G., Berger, T., Möller, R., Bohmeier, M., Leuko, S., Horneck, G., Westall, F., Jänchen, J., Fritz, J., Meyer, C., Onofri, S., Selbmann, L., Zucconi, L., Pacelli, C., Kozyrovska, N., Leya, T., Foing, B., Demets, R., Cockell, C.S., Bryce, C., Olsson-Francis, K., Wagner, D., Serrano, P., Edwards, H.G.M., Joshi, J., Huwe, B., Grossniklaus, U., Rövekamp, M., Ehrenfreund, P., Elsaesser, A., Schulze-Makuch, D., Feyh, N., Szewzyk, U., Ott, S., and Meessen, J.

Subjects
Strahlenbiologie, Mars analog field research, BIOMEX, biosignatures, Experimentelle Planetenphysik, space experiments, Mars simulation
Abstract

BIOMEX (Biology and Mars Experiment) is a space experiment on the exposure platform EXPOSER2 launched by the Progress 56 mission on 24 July and placed on the outer side of the Russian Zvezda Module of the International Space Station (ISS). Twenty-five international institutes are working together and sharing different methods, planetary simulation facilities, and logistics to obtain information about the vitality of the tested microorganisms and the stability of biomolecules as possible biosignatures. This experiment comprises three investigational steps from the field to space: (i) field work with sample collection and habitat characterization at field sites with or without Mars analogy, (ii) Mars simulation experiments in the lab and (iii) exposure to real space conditions. For the second and third steps some of the microorganisms and bio-molecules are embedded in Marsanalog regolith mixtures, placed in compartments enriched with Mars-like CO 2 -atmosphere and exposed to solar irradiation levels approaching those affecting the surface of Mars to test habitability on Mars, as well as the ability to detect the selected, Mars-exposed bio-molecules. One of the aims of this experiment is to investigate the specific bio-related spectra of resistant molecules obtained by fluorescence analysis, Raman-spectroscopy, IR- and UV/VIS spectrometry before and after simulated and real space exposure. The obtained database of stable bio-molecules will support future exploration missions to Mars whose main goal is the search for life.

Published
2014

46. Influence of housing system on behavioural and immune responses of buffalo heifers

Author

BILANCIONE, ANTONIO, DE ROSA, GIUSEPPE, GRASSO, FERNANDO, SABIA E., PACELLI C., NAPOLITANO F., Bilancione, Antonio, Sabia, E., DE ROSA, Giuseppe, Grasso, Fernando, Pacelli, C., and Napolitano, F.

Subjects
Buffalo heifer, behavioural response, housing system, immune responses
Abstract

From November 2005 to October 2006 thirty-two buffalo heifers were used to evaluate the effect of rearing system on behavioural and immune responses. Sixteen heifers (group IR) were group-housed in indoor slatted floor pen (3.0 m2/animal) with an outdoor paddock (3.0 m2/animal). Sixteen others (group ER) grazed a fenced Mediterranean maquis of ~ 40 ha (~ 600 m a.s.l.). The animals at beginning of experiment were aged 8-9 months and weighted 190 kg. Phytohaemagglutinin (PHA, 1 mg/animal) was used to perform a skin test based on non-specific delayed type hypersensitivity, whereas 5 mg of keyhole limpet hemocyanin (KLH) were injected subcutaneously to evaluate humoral immune response. At the end of the experimental period the animals were subjected to a novel object test. Each animal was exposed to a novel environment (a 6 x 6 m paddock), where in the middle a traffic cone was present. Avoidance distance at manger was evaluated by an assessor who walked slowly (1 step per second) toward each animal with one hand slightly forward until signs of withdrawal. Daily weight gain was higher in IR (0.56±0.02 kg/d) than ER (0.50±0.02 kg/d) animals, although puberty was reached at similar age (667 vs 675±11 d, for IR and ER respectively).During the novel object test, IR animals touched more and devoted more time to the traffic cone than ER animals. Avoidance distance at manger was lower in ER animals than IR. Skin thickness after PHA injection was higher in ER heifers than in IR, whereas the two groups showed a similar humoral immune response. The results indicated that an extensive rearing system based on pasture seems to be a valid method to promote welfare and sustainability of buffalo heifer.

Published
2008

47. Applicazione di un metodo di valutazione del benessere a livello aziendale in bovini da carne allevati in modo estensivo

Author

NAPOLITANO F., BRAGHIERI A., PACELLI C., ADDUCCI F., DE ROSA, GIUSEPPE, GRASSO, FERNANDO, Napolitano, F., DE ROSA, Giuseppe, Braghieri, A., Pacelli, C., Adducci, F., and Grasso, Fernando

Subjects
Animal Need Index, Benessere, Bovini da carne
Abstract

La ricerca è stata condotta al fine di verificare l’applicabilità del metodo Animal Need Index (ANI-35L) per la valutazione dello stato di benessere di bovini da carne. L’indagine ha interessato 30 aziende della regione Basilicata che allevano bovini di razza Podolica. Essendo i soggetti allevati in modo estensivo, per la valutazione del benessere a livello aziendale, oltre ad effettuare i rilievi riguardanti l’ambiente di allevamento e le condizioni dei ricoveri secondo quanto previsto dallo schema ANI, sono state prese in considerazione variabili rilevate direttamente sugli animali (stereotipie, grado di sudiciume, ferite, lesioni, zoppie, ecc.), in quanto la corretta interpretazione delle correlazioni esistenti tra queste ultime e gli aspetti che vengono considerati dal metodo di valutazione dell’ANI (possibilità di movimento, contatto sociale, tipologia e condizioni della pavimentazione, qualità dell’aria, ventilazione, luminosità, intensità dei rumori, management) può contribuire a rendere più precisa la valutazione del benessere animale. Inoltre, il rilievo dei range di ciascun parametro e la distribuzione delle aziende in quintili (5 classi di frequenza) ha consentito di definire una graduatoria di merito degli allevamenti, per cui è stato confermato che lo schema ANI, opportunamente integrato con determinati indicatori rilevati direttamente sugli animali, è in grado di fornire utili informazioni per il monitoraggio del benessere dei bovini da carne.

Published
2007

48. Follicular dynamics in superstimulated adult buffaloes

Author

NEGLIA, GIANLUCA, MASCHIO M., ALLEGRINI S., PACELLI C., MAZZI M., DE SANTIS G., BARILE V. L., TERZANO G. M., Neglia, Gianluca, Maschio, M., Allegrini, S., Pacelli, C., Mazzi, M., DE SANTIS, G., Barile, V. L., and Terzano, G. M.

Subjects
Follicular dynamic, FSH, Buffalo, Superovulation, PMSG
Abstract

The aim of this trial was to study the follicular dynamic in superovulated buffaloes. The trial was performed on 26 adult buffaloes synchronized by a double PGF2α injection 12 days apart. The day of estrus (Day 0) a progesterone releasing intravaginal device (PRID) was inserted and on day 7 the animals were divided in three homogeneous groups and assigned to one of the following treatments: 1) Group A (n=7) received a 4-days decreasing dose of an equal mixture of 1000 IU of FSH+LH; 2) Group B (n=9) was treated a single i.m. injection of 2000 IU of PMSG in a five days schedule; 3) Group C (n=10) received the same injection of PMSG and a 2-days decreasing dosage of 300 IU of FSH+LH, starting four days after PMSG. The PRID was removed the day before the end of the treatments in all groups and ultrasound examinations were performed daily starting on day 7 till the end of the treatments by a 7.5 MhZ linear rectal probe. Statistical analysis was carried out by GLM Procedures (SAS). Buffaloes in Group B showed a lower number (P10 mm) was recorded in Group B vs A and C (1.4 vs 3.1 and 4.4 respectively). It is worth pointing out that a significant (P

Published
2006

50. On the development of a monitoring scheme of buffalo at farm level

Author

DE ROSA, GIUSEPPE, GRASSO, FERNANDO, BORDI, ALDO, NAPOLITANO F., PACELLI C., DE ROSA, Giuseppe, Napolitano, F., Grasso, Fernando, Pacelli, C., and Bordi, Aldo

Subjects
Welfare, Monitoring system, Buffalo
Abstract

In the present review some animal-related indicators possibly relevant for a monitoring scheme of buffalo cow welfare at farm level are suggested. The indicators are discussed on the basis of their validity (meaningful with respect to animal welfare), reliability (reflecting the tendency to give the same results on repeated measurements) and feasibility (concerning time and money consume). The attention was focussed on the following indicators: excessive thinning or fattening assessed with Body Condition Score (BCS) systems; cleanliness; lameness; resting, social, oral abnormal behaviours; injuries; stockmanship; animal-human relationship; positive indicators (diversity of lying postures, allogrooming, etc.). We conclude that some of the indicators validated for cattle could be conveniently applied to buffaloes without changes (e.g. injuries and avoidance distance), others should be appropriately adjusted to this species (e.g. BCS, cleanliness, housing factors). In addition, further studies are needed either to find reliable and feasible registration methods for some indicators (e.g. aggressive and resting behaviours) or to identify additional parameters specific for buffaloes (e.g. prevalence of buffalo cows injected with oxytocin during milking as an indicator of the quality of human-animal relationship).

Published
2005

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